Anti-inflammatory carbon quantum dot particles for wound healing and repairing

Abstract

An anti-inflammatory carbon quantum dot particle for wound healing and repairing is torispherical and is applied to wound healing and repairing. The average particle size of the carbon quantum dot particles is 2.0 + / -0.5 nm, the hydrated particle size is 2.2 + / -0.4 nm, the Zeta potential is 36.3 + / -1.5 mV, EDTA-2Na and branched chain polyethyleneimine are dispersed in ultrapure water, the mixture is heated at 180 DEG C for 6-8 hours, and the carbon quantum dot particles are obtained after cooling and filtering. The carbon quantum dot particles have antibacterial and anti-inflammatory properties, can solve the two wound healing problems of bacterial infection and excessive inflammatory reaction at the same time, and remarkably improve the wound healing speed and quality.

Description

Technical Field

[0001] The present application relates to the field of material biotechnology, and in particular to a carbon dot for wound healing and repair. Background Art

[0002] In the field of wound healing and repair, traditional treatment methods mainly rely on the application of antibiotics, anti-inflammatory drugs and dressings. However, with the increase of drug-resistant microorganisms and the difficulty of treating chronic wounds, the effectiveness of traditional treatment methods has gradually been limited. In the fields of biomedicine and wound repair, traditional treatment methods have been unable to meet the growing medical needs, especially in combating drug-resistant microorganisms and promoting the healing of complex wounds. In recent years, carbon dots (CDs), as an emerging nanomaterial, have shown great potential in biomedical applications due to their excellent optical properties, good biocompatibility and stability, and adjustable surface functionalization capabilities. Carbon dots can not only be used as imaging probes for biomarkers and tracking, but can also achieve specific biological functions such as antibacterial and anti-inflammatory through surface modification, providing new possibilities for wound treatment.

[0003] Optical and biological properties of carbon dots: Carbon dots are usually composed of elements such as carbon, hydrogen, and oxygen, and have unique photoluminescent properties that can be used for biological imaging and sensing. By adjusting the size and surface structure of carbon dots, their luminescent wavelength can be controlled to achieve a wide range of coverage from visible light to near-infrared light. In addition, carbon dots are widely used in cell labeling, tissue imaging, and drug delivery due to their good water solubility, low toxicity, and good biocompatibility.

[0004] Application of carbon dots in biomedicine: As a multifunctional nanocarrier, carbon dots can carry drugs, genes or other bioactive molecules through surface modification to achieve targeted delivery and precision treatment. In wound treatment, carbon dots can be used as antibacterial agents to destroy bacterial cell walls or cell membranes through physical or chemical effects on their surfaces to achieve a bactericidal effect. At the same time, carbon dots can also be used as anti-inflammatory agents to promote wound healing by regulating inflammatory responses.

[0005] Defects of the existing technology: 1. Limitations of antibacterial and anti-inflammatory properties: Although carbon dots have shown many advantages in biomedical applications, their performance as antibacterial and anti-inflammatory agents still needs to be improved. Most existing carbon dots rely on physical destruction of their surfaces or achieve bactericidal effects through direct contact with bacteria, which limits their broad spectrum and efficiency in antibacterial properties. At the same time, the application of carbon dots in anti-inflammatory aspects is still in the initial exploration stage, and their anti-inflammatory mechanisms and effects need further research and verification.

[0006] 2. Consideration of biocompatibility and safety: Although carbon dots have good biocompatibility, carbon dots prepared by different methods and surface modification conditions may have different toxicity. Therefore, when using them for wound treatment, their biosafety and long-term effects need to be strictly evaluated.

[0007] 3. Preparation process and cost limitations: At present, the preparation process of carbon dots is relatively complex and costly, which limits their widespread clinical application. Simplifying the preparation process, reducing costs, and maintaining the superior performance of carbon dots are important directions for current research.

[0008] 4. Limitations of functional modification: Functional modification of carbon dots is the key to realize their biomedical applications. However, most existing functionalization methods rely on chemical bonding or physical adsorption, which may lead to the shedding or inactivation of functional molecules, affecting the stability and therapeutic effect of carbon dots.

[0009] In summary, although carbon dots show great potential in biomedical applications, there are still many challenges and defects in the field of wound treatment. The purpose of the present invention is to provide a carbon dot with excellent antibacterial and anti-inflammatory properties, by optimizing the preparation process, enhancing biocompatibility, improving stability and reducing costs, while realizing the multifunctional modification of carbon dots, to provide a new and more effective solution for wound treatment. Summary of the invention

[0010] The technical problem to be solved by the present invention is that in the prior art, wound healing and repair methods have limited effects when facing drug-resistant microorganisms and chronic wounds, and are often accompanied by excessive inflammatory responses, resulting in a delayed healing process. Although studies have explored the application of carbon quantum dots (CDs) in the antibacterial and biomedical fields, these studies often focus on a single function, such as simple antibacterial effects or antioxidant properties, while ignoring the dual needs of antibacterial and anti-inflammatory properties in the wound healing process. In addition, CDs in the prior art still have deficiencies in preparation conditions, functional regulation, and biocompatibility, making it difficult to meet the comprehensive requirements of intelligent wound management.

[0011] To achieve the above objectives, this application adopts the following technical solutions: An anti-inflammatory carbon quantum dot particle for wound healing and repair. The carbon quantum dot particle is quasi-spherical in shape and is used for wound healing and repair.

[0012] The average particle size of carbon quantum dot particles is 2.0±0.5 nm, the hydrated particle size is 2.2±0.4 nm, and the Zeta potential is 36.3±1.5 mV.

[0013] The atomic ratios of C1s, N1s and O1s in carbon quantum dot particles are 66.07%, 14.06% and 18.28%, respectively.

[0014] The preparation method comprises: dispersing EDTA-2Na and branched polyethyleneimine in ultrapure water, heating at 180° C. for 6-8 hours, cooling and filtering to obtain carbon quantum dot particles.

[0015] Add 0.56g of EDTA-2Na and 0.14g of branched polyethyleneimine to every 15mL of ultrapure water, use ultrasonic dispersion to make it uniform and without precipitation, then heat it. After heating, let it stand at room temperature for a while.

[0016] The carbon quantum dot particles were diluted with water to 300ug / mL and then applied to the wound.

[0017] Compared with the prior art, this application has the following advantages: Dual functions, synergistic effects: PECDs have both antibacterial and anti-inflammatory properties, which can simultaneously solve the two major wound healing problems of bacterial infection and excessive inflammatory response, and significantly improve the speed and quality of wound healing. Good biocompatibility and high safety: PECDs and their combination with GelMA hydrogel showed good biocompatibility in both in vitro and in vivo experiments, reducing the potential risks during treatment. Promote macrophage polarization and accelerate healing: PECDs can regulate the polarization of macrophages from M1 type to M2 type, reduce the release of inflammatory factors, promote angiogenesis and collagen deposition, thereby accelerating the wound healing process. The preparation process is simple and easy to promote: The hydrothermal synthesis process adopted in the present invention is simple and easy, which is conducive to the large-scale production and wide application of PECDs. In summary, the anti-inflammatory carbon quantum dot particles provided by the present invention and their application in wound healing and repair provide a new solution to the problems existing in the prior art, with significant technical innovation and broad market application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 Structural characterization of PECDs TEM image of PECDs, showing the uniform distribution and nanoscale size of PECDs; and HR-TEM image of PECDs, further revealing the fine structure and lattice fringes of PECDs.

[0019] Figure 2 The in vitro antibacterial properties of PECDs are shown in the figure. The plate counting method is used to statistically analyze the bactericidal effects of PECDs on Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) under different pH conditions (pH 3-9).

[0020] Figure 3The results of the antibacterial zone experiment of PECDs intuitively demonstrate the inhibitory effect of PECDs on bacteria. The figure shows the bacterial growth after PECDs treatment. Compared with the untreated control group, the PECDs treatment group showed an obvious inhibition zone, indicating that PECDs has excellent antibacterial properties.

[0021] Figure 4 is the bactericidal effect of the MIC concentration of PECDs.

[0022] Figure 5 Evaluation of the in vitro antioxidant activity of PECDs: Histogram of iron ion chelation ability analysis. This figure shows the chelation ability of PECDs for iron ions, which is an important indicator for evaluating its antioxidant properties.

[0023] Figure 6 Evaluation of the in vitro antioxidant activity of PECDs: Analysis of intracellular superoxide dismutase (SOD) activity after co-culture of macrophages with PECDs (300 μg / mL). This experiment aims to explore the effect of PECDs on SOD activity in macrophages, thereby evaluating its antioxidant properties. And the changes in the scavenging effect of PECDs on ·O2- with pH value. This figure shows the scavenging ability of PECDs on ·O2- under different pH conditions, which helps to understand the antioxidant properties of PECDs in different environments.

[0024] Figure 7 Evaluation of the in vitro antioxidant activity of PECDs: The CAT (catalase)-like activity of PECDs catalyzes the production of oxygen from H2O2. This figure shows the antioxidant properties of PECDs as CAT-like enzymes that can catalyze the decomposition of H2O2 into oxygen and water, thereby removing hydrogen peroxide. And the scavenging efficiency of PECDs for H2O2 at different pH levels. This experiment evaluated the scavenging ability of PECDs for H2O2 under different pH conditions, which helps to understand the antioxidant properties of PECDs in different environments.

[0025] Figure 8 ESR spectra of ·OH for evaluating the in vitro antioxidant activity of PECDs. Through ESR spectroscopy, the scavenging effect of PECDs on hydroxyl radicals (·OH) can be observed, further verifying its antioxidant properties. And the scavenging efficiency of PECDs on hydroxyl radicals at different pH levels. This figure shows the scavenging ability of PECDs on ·OH under different pH conditions, which helps to understand the antioxidant properties of PECDs in different environments.

[0026] Fig. 9The scavenging effect of PECDs on ROS in LPS-induced macrophages was observed by fluorescence microscopy, further verifying its antioxidant properties.

[0027] Fig.10 This experiment analyzed the role of PECDs in regulating LPS-induced macrophage polarization by flow cytometry, further revealing the mechanism of its anti-inflammatory and antioxidant properties.

[0028] Fig.11 To evaluate the biocompatibility of PECDs: The survival rate of HaCaT cells after PECDs treatment was evaluated by CCK8 assay.

[0029] Fig.12 To evaluate the hemolytic activity of PECDs in PBS, PBS-treated red blood cells (RBCs) were used as negative controls, and H2O-treated RBCs were used as positive controls. Error bars represent the standard deviation of three replicates. This section aims to verify the potential toxic or destructive effects of PECDs on RBCs by comparing their performance in PBS (expected to have no effect) and H2O (expected to cause RBC rupture).

[0030] Fig.13 Representative photos of PECDs in promoting wound healing in infected mice. The photos show the wound conditions after PBS or PECDs treatment for 0, 3, 6, and 9 days. By taking photos of the wounds and calculating the percentage of wound area, the promotion of wound healing by PECDs can be intuitively observed.

[0031] Fig.14 H&E staining and Masson trichrome staining of wound tissue. Through histological staining techniques, the morphological structure of wound tissue and the distribution of collagen fibers can be observed, thereby evaluating the effect of PECDs on wound healing.

[0032] Fig.15 The results of immunohistochemical staining. Immunohistochemical staining was performed on mice treated with PBS or PECDs for 3, 6, and 9 days to detect the expression of angiogenic factor CD31 and inflammatory factors IL-6 and TNF-α. The effects of PECDs on angiogenesis and inflammatory response in wound tissues can be observed through immunohistochemical staining techniques. DETAILED DESCRIPTION

[0033] In order to make the purpose, technical solution and advantages of the present application more clear, the present application is further described in detail below. However, it should be understood that the description here is only used to explain the present application and is not used to limit the scope of the present application.

[0034] Unless otherwise defined, all technical terms and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present application, and the terms used herein in the specification of the present application are only for the purpose of describing specific embodiments and are not intended to limit the present application. The reagents and instruments used herein are all commercially available, and the characterization means involved can refer to the relevant descriptions in the prior art, which will not be repeated herein.

[0035] Example 1 The present invention proposes an anti-inflammatory carbon quantum dot particle (PECDs) for wound healing and repair. The particle is synthesized by a hydrothermal method and optimized under specific synthesis conditions to obtain optimal -NH2 functionalization, thereby enhancing bacterial adhesion and antibacterial activity. Specifically, the synthesis of PECDs is accomplished by dispersing 0.14 g of polyethyleneimine (PEI) in 15 mL of ultrapure water, adding 0.56 g of EDTA-2Na, and ultrasonically dispersing for 30 minutes until a uniform and precipitate-free state is obtained. The reaction vessel is set to a temperature of 180°C for heating for 6 hours, and allowed to stand at room temperature overnight. Characterization by transmission electron microscopy (TEM) and high-resolution transmission electron microscopy (HR-TEM) revealed that the uniform nanostructure and appropriate particle size distribution ( Figure 1 ).

[0036] Example 2 In order to evaluate the antibacterial properties of PECDs, Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were used as test strains in this study. Furthermore, the antibacterial ability of PECDs was evaluated under different pH conditions using the plate count method. The results showed that at lower pH values, the antibacterial effect of PECDs was more significant, and the antibacterial effect on S. aureus was better than that on E. coli. This may be due to the stronger negative charge on the surface of S. aureus, which leads to a more obvious electrostatic adsorption effect of PECDs on it ( Figure 2 ).

[0037] In order to determine the minimum inhibitory concentration (MIC) of PECDs against different bacterial strains, the standard dilution method was used in this study. As the pH value decreased, the MIC values ​​of PECDs against E. coli and S. aureus decreased, ranging from 150 to 300 μg / mL. The bactericidal efficiency of PECDs was further verified by plate count experiments. The results showed that under the conditions of pH 3 and pH 5, 200 μg / mL of PECDs could achieve a 99% killing rate against S. aureus. At the same time, the minimum bactericidal concentration (MBC) of PECDs against S. aureus was 300 μg / mL in the range of pH 3 to pH 9, while the MBC for E. coli needed to reach 400 μg / mL at pH 7-9.

[0038] The results of the inhibition zone assay further confirmed that the antibacterial effect of PECDs was related to pH value, and the antibacterial effect on S. aureus was stronger. Therefore, in order to obtain the best antibacterial effect, this study selected PECDs under pH 5 as candidate antibacterial agents for subsequent research ( Figure 3 ).

[0039] Subsequently, this study used MIC concentrations of PECDs to co-incubate with E. coli and S. aureus, and stained with the fluorescent dye SYTO / PI. The staining results showed that the control group showed bright green fluorescence and was evenly distributed, while the PECDs-MIC treatment group showed red fluorescence, and the aggregation of S. aureus was more obvious, indicating that the bacterial membrane was damaged and most of the bacteria were dead ( Figure 4 ). This finding is consistent with the results of the plate count experiment, further confirming that PECDs have excellent antibacterial properties.

[0040] In summary, PECDs showed strong antibacterial effects, and their antibacterial ability was enhanced as the pH value decreased. These findings provide strong support for the application of PECDs as a new antibacterial material in wound healing and repair.

[0041] Example 3 The present invention proposes a polyethylene glycol carbon quantum dot (PECDs) with significant in vitro antioxidant activity. The quantum dots are prepared by a specific synthesis process and exhibit excellent metal ion chelating ability and superoxide dismutase (SOD)-like and catalase (CAT)-like activities, as well as hydroxyl radical (·OH) scavenging ability. The following is a detailed analysis of the synthesis of PECDs and their antioxidant activity.

[0042] 1. Metal ion chelating ability The metal ion chelation experiment of PECDs showed that its chelation effect on Fe²⁺ varies with concentration and pH. Specifically, at a concentration of 500 μg / mL, PECDs can chelate about 70% of Fe²⁺ under acidic conditions, increase to about 80% under neutral conditions, and approach 90% under alkaline conditions of pH 9 ( Figure 5 ). This result indicates that the metal ion chelating ability of PECDs increases with the increase of pH, which may be related to the weakening of electrostatic repulsion.

[0043] 2. SOD-like enzyme activity and superoxide radical scavenging ability Superoxide generated by the xanthine-xanthine oxidase system was converted into hydrogen peroxide (H2O2) by PECDs at different pH conditions. 2 O 2 ), and detect H by monitoring the absorbance change 2 O 2 In macrophages, the activity of SOD-like enzymes increased from about 12 U / mL to about 28 U / mL after PECDs treatment, almost 2 ⁻ Double the removal efficiency ( Figure 6 ). Meanwhile, the increase of pH leads to the increase of ·O 2 ⁻The scavenging effect and SOD-like enzyme activity gradually weakened ( Figure 6 ), and this activity is closely related to the concentration of PECDs.

[0044] 3. CAT-like activity and hydrogen peroxide scavenging ability PECDs also exhibit significant CAT-like activity by decomposing H 2 O 2 H 2 O and O 2 The experimental results showed that as the pH decreased, the CAT-like activity of PECDs increased and the dissolved oxygen content gradually increased ( Figure 7 ). Meanwhile, PECDs exhibited higher H 2 O 2 The removal efficiency increased with the increase of concentration ( Figure 7 ).

[0045] 4. Hydroxyl free radical scavenging ability Using the ·OH generated by the Fenton reaction, the EPR detection results showed that the ·OH signal was significantly reduced after PECDs treatment, indicating that it has good ·OH scavenging ability. This scavenging ability seems to be independent of pH, but is positively correlated with the concentration of PECDs ( Figure 8 ).

[0046] In summary, the PECDs proposed in the present invention exhibit excellent in vitro antioxidant activities, including metal ion chelation, SOD-like and CAT-like enzyme activities, and ·OH scavenging ability, through their unique synthesis process and surface functional groups, providing new candidate materials for antioxidant therapy.

[0047] Example 4 Antioxidant and anti-inflammatory effects of PECDs in a cellular inflammation model Establishment of cell inflammation model: 200 μM H 2 O 2 The cell inflammation model was successfully established by treating cells. The degree of inflammatory response was evaluated by measuring the level of intracellular ROS (reactive oxygen species) ( Fig. 9 ).

[0048] Antioxidant effect of PECDs: After cells were treated with 200 μg / mL of PECDs, it was found that PECDs could significantly reduce intracellular ROS levels. This reduction was concentration-dependent and was evident in the pH 3-7 range. Especially under neutral conditions (pH 7), PECDs were able to reduce ROS levels in inflammatory cells to levels close to those of normal cells. Although the ROS scavenging efficiency of PECDs was relatively low at pH 3 and pH 9, this helped maintain the balance of ROS, which is critical for wound healing.

[0049] pH-dependent mechanism of PECDs: Studies have shown that PECDs have cascade SOD-CAT nanozyme activity and hydroxyl radical scavenging ability. This mechanism shows a certain pH dependence and may be a protective mechanism for PECDs to remove ROS. In the early stages of bacterial infection, ROS helps to kill bacteria; while in the stages of cell proliferation and extracellular matrix remodeling, a moderate level of ROS is essential for tissue repair. PECDs can adjust their antioxidant activity with changes in pH, thereby maintaining the balance of ROS and promoting tissue repair.

[0050] Anti-inflammatory effect of PECDs: A macrophage inflammation model was established by treating RAW264.7 cells with LPS. Fluorescence microscopy results showed that PECDs could significantly reduce the fluorescence signal intensity in inflammatory cells, indicating that it has efficient ROS scavenging ability. Flow cytometry results showed that PECDs could reduce the number of CD86+F4 / 80+ cells (pro-inflammatory M1 macrophages) and increase the number of CD206+F4 / 80+ cells (anti-inflammatory M2 macrophages). This shows that PECDs can regulate the phenotype and function of macrophages, promote the polarization of macrophages from M1 to M2, and thus reduce the inflammatory response ( Fig.10 ).

[0051] This study demonstrated that PECDs have significant antioxidant and anti-inflammatory effects through cell inflammation model experiments. PECDs can remove intracellular ROS, regulate the phenotype and function of macrophages, and reduce the expression level of inflammatory factors, thereby alleviating inflammatory responses and promoting wound healing. These findings provide strong support for the application of PECDs in the treatment of related diseases.

[0052] Example 5 Biocompatibility evaluation of PECDs materials In the field of biological applications, the biocompatibility of materials is crucial. This study used the CCK-8 method to evaluate the cytotoxicity of PECDs to HaCaT cells for the first time, thereby deeply exploring its biocompatibility.

[0053] Experimental methods and results: CCK-8 cytotoxicity evaluation: The study selected different concentrations of PECDs to treat HaCaT cells and tested the cell survival rate after 24 hours and 48 hours. The results showed that even when the PECDs concentration was 2500 μg / mL, the cell survival rate remained above 85% (see Fig.11 ). This data fully proves that PECDs have excellent biocompatibility.

[0054] In vitro hemolysis test: In order to further verify the biocompatibility of PECDs, the study also conducted an in vitro hemolysis test. The test results showed that the hemolysis rate of PECDs was less than 5% at all tested concentrations, and the hemolysis rate was less than 2% at the minimum inhibitory concentration (MIC) of PECDs (see Fig.12 ). This result further confirms the safety of PECDs in biological applications.

[0055] In summary, this study comprehensively evaluated the biocompatibility of PECDs materials through multiple methods such as CCK-8 cytotoxicity assessment, in vitro hemolysis test, and Calcein / PI live-dead fluorescence staining observation. The results showed that PECDs exhibited excellent biocompatibility at various concentrations, had extremely low toxicity to HaCaT cells, and the hemolysis rate was also kept within a safe range. These findings provide strong support for the application of PECDs in the biomedical field.

[0056] Example 6 Application of PECDs in promoting wound healing and its biosafety assessment The present invention relates to the application of PECDs (a material with antibacterial and antioxidant properties) in promoting wound healing, and verifies its effectiveness and biosafety through experiments.

[0057] The wound healing process includes four key stages: hemostasis, inflammation, cell proliferation, and extracellular matrix remodeling. However, during the inflammatory phase, bacteria such as Staphylococcus aureus (S. aureus) are prone to cause wound infection, leading to excessive production of reactive oxygen species (ROS), which in turn affects cell proliferation and delays wound healing. To address this issue, this study used a wound infection mouse model to evaluate the in vivo antibacterial ability of PECDs (see Fig.13 ).

[0058] In the experiment, a 7mm diameter wound was made on the back of the mouse and infected with S. aureus. After 24 hours, abscesses appeared on the surface of the wound, which was then treated with PBS and PECDs, respectively, and the number of bacteria in the wound site was detected. The results showed that the number of pustules in the PECDs group decreased, the wound area was reduced, and between the 6th and 9th days, the wound area in the PECDs group was significantly different from that in the control group (p<0.05). By the 9th day, the wound in the PECDs group was almost completely healed. At the same time, the number of bacteria in the PECDs group was significantly reduced, proving that PECDs has a sustained antibacterial effect during wound healing and promotes wound healing (see Fig.13 ).

[0059] The wound tissue healing quality and collagen deposition were further evaluated by H&E staining and Masson trichrome staining. The results showed that the PECDs treatment group had reduced inflammatory cell infiltration, tight collagen fiber arrangement, and significant collagen deposition, indicating that PECDs promoted wound repair and rapid recovery (see Fig.14 ). In addition, PECDs treatment also led to an increase in the level of platelet endothelial cell adhesion molecule-1 (CD31) in the wound tissue, indicating an increase in angiogenesis, while reducing the levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), reducing inflammatory factors, and promoting the start of the cell proliferation phase. The expression levels of M1 cytokines (IL-1β, TNF-α) and M2 cytokines (IL-10, TGF-β1, VEGF) in the wound tissue were detected by qPCR. It was found that the expression of M1 cytokines in the PECDs group was significantly reduced on the 9th day, and the expression of M2 cytokines was increased, indicating that PECDs helps accelerate the healing process and promotes the polarization of M1 macrophages (see Fig.15 ).

[0060] In summary, PECDs exhibit significant antibacterial and antioxidant properties in the wound healing process, accelerating wound healing by reducing inflammation, promoting collagen formation and angiogenesis. At the same time, PECDs have good biosafety, providing broad prospects for its application in the biomedical field.

Claims

1. An anti-inflammatory carbon quantum dot particle for wound healing and repair, the carbon quantum dot particle is quasi-spherical in shape, characterized in that: Used in wound healing and repair.

2. The anti-inflammatory carbon quantum dot particles for wound healing and repair according to claim 1, characterized in that: The average particle size of carbon quantum dot particles is 2.0±0.5nm, the hydrated particle size is 2.2±0.4nm, and the Zeta potential is 36.3±1.5mV.

3. The anti-inflammatory carbon quantum dot particles for wound healing and repair according to claim 1, characterized in that: The atomic ratios of C1s, N1s and O1s in the carbon quantum dot particles are 66.07%, 14.06% and 18.28%, respectively.

4. The anti-inflammatory carbon quantum dot particles for wound healing and repair according to claim 1, characterized in that The preparation method comprises: dispersing EDTA-2Na and branched polyethyleneimine in ultrapure water, heating at 180°C for 6-8 hours, cooling and filtering to obtain carbon quantum dot particles.

5. The anti-inflammatory carbon quantum dot particles for wound healing and repair according to claim 4, characterized in that: Add 0.56g of EDTA-2Na and 0.14g of branched polyethyleneimine to every 15mL of ultrapure water, use ultrasonic dispersion to make it uniform and free of precipitation, then heat it. After heating, let it stand at room temperature for a while.

6. The anti-inflammatory carbon quantum dot particles for wound healing and repair according to any one of claims 1 to 5, characterized in that: The carbon quantum dot particles were diluted with water to 300ug / mL and then applied to the wound.

7. The anti-inflammatory carbon quantum dot particles for wound healing and repair according to any one of claims 1 to 5, characterized in that: Add carbon quantum dot particles into the hydrogel.

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