Traditional Chinese medicine composition for treating depression and preparation method and application thereof

Abstract

The invention provides a traditional Chinese medicine composition for treating depression and a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine compositions.The traditional Chinese medicine composition is prepared from platycladi seeds, radix bupleuri, gastrodia elata, radix paeoniae alba, turmeric, cicada slough, honey-fried licorice roots, rhizoma zingiberis, szechwan chinaberry fruits, coptis chinensis, fructus evodiae, cortex albiziae, ligusticum wallichii and gynostemma pentaphylla. The invention also provides a preparation method of the traditional Chinese medicine composition, which comprises the following steps: distilling the semen boitae, the ligusticum wallichii, the dried ginger and the curcuma zedoary to obtain volatile oil and first medicine residues, and inclusion the volatile oil and beta-cyclodextrin to prepare volatile oil dry powder; performing primary alcohol extraction on radix bupleuri, radix paeoniae alba, cortex albiziae, gynostemma pentaphylla and the first medicine residue; performing secondary alcohol extraction on coptis chinensis, fructus evodiae, szechwan chinaberry fruit and turmeric; performing enzymolysis on honey-fried licorice root and gastrodia elata, and performing water extraction; and concentrating, eluting and granulating to obtain the traditional Chinese medicine composition. The composition is good in treatment effect and free of dependence, and the preparation method is high in medicine extraction rate.

Description

Technical Field

[0001] The present invention belongs to the technical field of Chinese medicine compositions, and in particular relates to a Chinese medicine composition for treating depression, and a preparation method and application thereof. Background Art

[0002] An important neurobiological mechanism of depression is neurotransmitter imbalance. Currently, there are many studies on monoamine neurotransmitters. It is believed that the onset of depression is related to the decline of monoamine neuron function in the brain and the obstruction of monoamine neurotransmitter synthesis and transport. Studies have found that the levels of serotonin (5-HT), dopamine (DA) and norepinephrine (NE) in the brains of patients with depression, especially those who commit suicide, are reduced. In addition, there are multiple interactions between these three neurotransmitters, which lead to problems such as low mood, loss of interest and lack of motivation. In recent years, it has been found that overactivation of the hypothalamus-pituitary-adrenal axis (HPA axis), neuroinflammation (increased cytokines such as IL-6 and TNF-α) and changes in brain structure (atrophy of the prefrontal cortex and reduction of hippocampal volume) are also involved in the pathological process. On the psychological level, negative cognitive patterns (such as Beck's triad: negative cognition of self, world, and future) and high neurotic personality (NEO scale score ≥ 60 points) are core susceptibility factors. At the social environment level, chronic stress (such as long-term unemployment, marital breakdown), traumatic events and lack of social support directly trigger the disease.

[0003] Most of the existing drugs for treating depression are chemical drugs, such as fluoxetine and esketamine. Although they have good therapeutic effects, they have great side effects, such as strong drug dependence. In comparison, Chinese medicine formula drugs have fewer side effects and are not addictive. Common Chinese medicine compound prescriptions for treating depression include liver-soothing and depression-relieving drugs: Xiaoyao Powder (Bupleurum, White Peony Root, Chinese Angelica), Chaihu Shugan Powder (Bupleurum, Cyperus rotundus, Chuanxiong).

[0004] The category of calming the mind and calming the spirit: Ganmai Dazao Decoction (licorice, wheat, jujube), Tianwang Buxin Dan. The category of nourishing the deficiency and strengthening the body: Guipi Decoction (astragalus, codonopsis, longan meat). In the prior art, the effective ingredients of traditional Chinese medicine are often extracted by decoction, and the extraction rate is low. It can be seen that the effect of the traditional Chinese medicine formula in the prior art for treating depression is poor, the extraction rate of effective ingredients is low, and the drug takes effect slowly. Summary of the invention

[0005] In view of this, the present invention provides a Chinese medicine composition for treating depression and a preparation method and application thereof, so as to solve the technical problems in the prior art that Chinese medicine prescriptions for treating depression have poor effects, low extraction rates of effective ingredients, and slow drug onset.

[0006] To achieve the above-mentioned purpose of the invention, the present invention provides a traditional Chinese medicine composition for treating depression, the raw materials of which include: cypress seed, bupleurum, gastrodia elata, white peony root, turmeric, cicada shell, roasted licorice, dried ginger, toosendan fruit, coptis chinensis, evodia rutaecarpa, julibrissin bark, ligusticum chuanxiong and gynostemma pentaphyllum.

[0007] Preferably, the raw materials of the traditional Chinese medicine composition also include Curcuma zedoaria.

[0008] Preferably, the raw materials include, by mass: 11-15 parts of pine nuts, 5-7 parts of bupleurum, 3-5 parts of gastrodia elata, 3-5 parts of white peony root, 5-7 parts of turmeric, 2-5 parts of cicada shells, 3-6 parts of roasted licorice, 3-4 parts of dried ginger, 3-5 parts of toosendan fruits, 3-6 parts of coptis chinensis, 3-5 parts of evodia rutaecarpa, 5-7 parts of gynostemma pentaphyllum and 5-7 parts of zedoaria.

[0009] Preferably, the average particle size of the pine nuts, bupleurum, gastrodia, white peony root, turmeric, cicada shell, licorice, dried ginger, toosendan fruit, coptis root, evodia rutaecarpa, julibrissin bark, ligusticum chuanxiong, gynostemma pentaphyllum and zedoaria are all 0.6-0.7 mm.

[0010] The antioxidant capacity of curcumin in turmeric can scavenge free radicals, reduce neuronal oxidative damage, improve the redox balance in the brain, and thus alleviate depression-related neuropathological changes. Curcumin can improve the level of 5-HT in the synaptic cleft by regulating the expression or function of 5-HT receptors (such as 5-HT1A, 5-HT1B), thereby alleviating depression. Curcumin can reverse the low expression of 5-HT1B in the hippocampus of depression model mice. Curcumin may indirectly improve mood regulation function by regulating the metabolism or receptor sensitivity of dopamine and norepinephrine. Curcumin can activate the cAMP / PKA / CREB signaling pathway and upregulate the expression of brain-derived neurotrophic factor (BDNF). BDNF is a key regulator of neuroplasticity that can promote neuronal survival, synapse formation, and neural regeneration, which is crucial for brain function repair in patients with depression.

[0011] Bupleurum's main ingredient, saikosaponin, can regulate the hypothalamic-pituitary-adrenal axis (HPA axis), relieve stress response, lower cortisol levels, and improve mood disorders.

[0012] The albizzia saponins and flavonoids in the bark of Albizzia julibrissin can inhibit the overexcitement of the central nervous system, regulate neurotransmitters such as 5-hydroxytryptamine (5-HT), and exert a sedative and anti-anxiety effect. It works in synergy with Bupleurum chinense to enhance the effects of soothing the liver and relieving depression, and calming the mind, and relieve insomnia and irritability associated with depression. The bark of Albizzia julibrissin can relieve the inhibitory effect caused by stress, regulate the level of monoamine neurotransmitters in the brain, and exert an antidepressant effect.

[0013] Ingredients such as ligustrazine in Chuanxiong can improve brain blood circulation, increase cerebral blood flow, promote nerve cell repair, and regulate the levels of monoamine neurotransmitters (such as dopamine and norepinephrine). When used in combination with Albizzia Julibrissin, Bupleurum Root, and Curcuma Atractylodes, it can dredge qi and blood stasis and improve physical symptoms such as headache and chest tightness associated with depression. The blood-brain barrier is an important obstacle for drugs to enter the brain. The active ingredients in Chuanxiong (such as ligustrazine) can indirectly improve the distribution of drugs in the brain by regulating the tight junction proteins of the blood-brain barrier, moderately increasing its permeability and increasing cerebral blood flow, thereby making it easier for the effective ingredients for treating depression to enter the brain tissue and improving the therapeutic effect of traditional Chinese medicine.

[0014] Gynostemma pentaphyllum saponins in Gynostemma pentaphyllum have antioxidant and anti-inflammatory effects, can regulate the HPA axis, inhibit neuroinflammation, and activate brain-derived neurotrophic factor (BDNF), promote neuronal regeneration, and improve depression. It works synergistically with Bupleurum chinense and Albizzia julibrissin to enhance the anti-depressant effect from the perspective of neuroprotection and anti-inflammatory.

[0015] Curcumene and curcumone in the volatile extracts of Curcuma have significant in vitro antioxidant capacity, which can effectively alleviate the neurobehavior of depression, reduce the expression of oxidative damage markers (ROS, MDA) in hippocampal tissue, increase the activity of antioxidant enzymes (SOD, CAT, GSH) and regulate the GPX and PRDX antioxidant systems, improve hippocampal neuron damage and mitochondrial dysfunction, thereby relieving oxidative stress in depression and exerting an antidepressant effect. When combined with Chuanxiong, it can enhance the effect of promoting blood circulation and qi, solve the pathogenesis of depression, and improve symptoms such as low mood and fatigue.

[0016] The composition of the present invention uses compound saponins, such as saikosaponins in bupleurum (such as saikosaponins a and d), albizzia saponins in Albizzia bark (such as Albizzia saponin A), and Gynostemma pentaphyllum saponins (such as Gynostemma pentaphyllum saponins XVII). These saponin components exert antidepressant effects through different mechanisms. The antidepressant effect of saikosaponins is to exert neuroprotective effects by positively regulating the BDNF-TrkB pathway, thereby inhibiting the neural damage caused by the development of inflammation. The glial cells in the brains of depression suicide patients are in an activated state. Glial cells mainly exist in the form of microglia and astrocytes. Microglia interact with astrocytes, regulate the changes of inflammatory factors and inflammatory mediators, produce anti-inflammatory effects, and restore tissue homeostasis to maintain normal physiological functions. It was observed that Gynostemma pentaphyllum saponins can reduce the release of inflammatory factors and promote the phenotypic transformation of microglia, thereby achieving antidepressant effects. Albizzia saponins have a certain protective effect on damaged neuronal cells and have the ability to resist oxidative stress. The use of the three saponins together can enhance the antidepressant effect and therapeutic stability.

[0017] A method for preparing a Chinese medicine composition for treating depression according to the present invention comprises the following steps: S1, mixing Platycladus orientalis seeds, Rhizoma Chuanxiong, dried ginger and Rhizoma Curcumae to obtain a distilled powder, placing the distilled powder into a steam distillation apparatus for distillation, wherein the ratio of the distilled powder to distilled water is 1 g: 8-10 L, and the distillation time is 4-6 h, to obtain volatile oil and a first medicinal residue; S2, mixing volatile oil: β-cyclodextrin: water in a ratio of 1 g: 8 - 12 g: 160-240 ml, stirring at 40-60° C. for 1-2 hours for inclusion, and freeze-drying to prepare volatile oil dry powder; S3, adding bupleurum root, white peony root, Albizzia julibrissin, Gynostemma pentaphyllum and the first medicinal residue into a first ethanol solution with a volume percentage of 70-75%, extracting by reflux at 80-85° C., and filtering to obtain a first alcohol extraction solution and a second medicinal residue; S4, adding coptis root, evodia rutaecarpa, toosendan fruit and turmeric to the second ethanol solution, condensing and refluxing at 60-65° C. after ultrasonication, and filtering to obtain the second ethanol extraction solution and the third medicinal residue; S5, mixing the washed and dried third medicinal residue with roasted licorice and Gastrodia elata, adding the mixture into water at a solid-liquid ratio of 1 g:12-15 ml, adding cellulase for enzymolysis to obtain an enzymolysis mixture, performing dynamic countercurrent extraction at 95-100° C., and obtaining a first water extraction solution after solid-liquid separation; S6, mixing the first alcohol extraction solution and the second alcohol extraction solution and concentrating under reduced pressure at 60° C. to obtain a first concentrated solution; S7, concentrating the first aqueous solution under vacuum at 60° C. to obtain a second concentrated solution; S8. After mixing the first concentrated solution and the second concentrated solution, the mixture is subjected to solvent elution using an AB-8 type macroporous resin column to obtain a purified extract, which is granulated and mixed with volatile oil dry powder to obtain the Chinese medicine composition.

[0018] Preferably, the material-liquid ratio during the reflux extraction in S3 is 1 g:10 ml.

[0019] Preferably, the total mass of the coptis root, evodia rutaecarpa, toosendan fruit and turmeric in S4 and the second ethanol solution has a solid-liquid ratio of 1g:8-10ml; Preferably, the second ethanol solution in S4 is a mixed solution of 0.1 vol% HCl-60 vol% ethanol. The mixed solution of 0.1 vol% HCl-60 vol% ethanol here means that 100 ml of the second ethanol solution contains 0.1 ml of hydrochloric acid and 60 ml of ethanol.

[0020] Medicinal materials such as Coptis chinensis, Evodia rutaecarpa, and Toosendan fruit contain free alkaloids (alkaline substances) that are difficult to dissolve in ethanol. After adding hydrochloric acid, the alkaloids and HCl form salts (such as berberine hydrochloride), which significantly improves the solubility in acidic ethanol. After extraction, the alcohol extract needs to be concentrated under reduced pressure (below 60°C). The boiling point of HCl is -85°C, and more than 95% evaporates during the concentration stage. Subsequent macroporous resin purification (water washing + alcohol washing) further removes the residue, and the residual HCl in the final preparation is less than 0.01%, which meets the requirements and does not cause residual hydrochloric acid.

[0021] Preferably, the mass of the cellulase in S5 is 0.5%-0.7% of the enzymatic hydrolysis reactant, the enzymatic hydrolysis temperature is 40-45° C., and the enzymatic hydrolysis time is 1-1.5 h.

[0022] A pill for treating depression prepared with the traditional Chinese medicine composition of the invention.

[0023] In the preparation method of the present invention, different preparation methods are used for different effective ingredients. The volatile oil is collected by steam distillation, which can effectively extract the effective ingredients in Platycladus orientalis, Ligusticum chuanxiong, dried ginger and Curcuma zedoaria. In the alcohol extraction process, a "two-step method" is used to extract the effective ingredients in traditional Chinese medicine, and ethanol is directly used to extract saponins / flavonoids, such as saponins in Bupleurum, White Peony, Albizzia julibrissin, and Gynostemma pentaphyllum; acidic ethanol (0.1% HCl - 60% ethanol) is used as an extraction solvent to extract alkaloids / fat-soluble components, such as alkaloids and fat-soluble components in medicinal materials such as Coptis chinensis, Evodia rutaecarpa, Toosendan fruit, and Curcuma longa. Multiple extractions can improve the extraction rate of effective ingredients and avoid the destruction of ingredients due to long single extraction time. The water extraction process is used to extract the water-soluble components in Radix Glycyrrhizae Radix and Gastrodia elata, and enzymatic hydrolysis is performed first and then water extraction is performed to improve the dissolution rate of the effective ingredients.

[0024] Compared with the prior art, the present invention has the following beneficial effects: In the composition of the present invention, turmeric relieves neuropathological changes related to depression, bupleurum relieves stress response, improves mood disorders, gynostemma promotes neuronal regeneration, improves depression, zedoaria can effectively relieve the neurobehavior of depression, julibrissin bark can regulate the monoamine neurotransmitter level of the brain, chuanxiong can improve brain blood circulation, increase cerebral blood flow, promote nerve cell repair, increase cerebral blood flow, indirectly improve the distribution of drugs in the brain, and improve the therapeutic effect of Chinese medicine. And using composite saponins, antidepressant effects are exerted through different mechanisms, and depression is treated in multiple dimensions from behavior, emotion, and neurotransmitter secretion, with good therapeutic effect and no dependence. The preparation method of the present invention, by means of distribution and purification, extracts the effective ingredients of different Chinese medicines, can greatly improve the dissolution of effective ingredients of Chinese medicines, improve the extraction rate of drug ingredients, and improve the therapeutic effect of depression. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical solution and advantages of the embodiment of the present invention clearer, the technical solution of the embodiment of the present invention will be clearly and completely described below. Obviously, the described embodiment is a part of the embodiment of the present invention, not all of the embodiments. Based on the described embodiment of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] Example 1 This embodiment provides a Chinese medicine composition for treating depression, wherein the raw materials include, by weight: 11 parts of Semen Platycladi, 5 parts of Radix Bupleuri, 3 parts of Rhizoma Gastrodiae, 3 parts of Radix Paeoniae Alba, 5 parts of Rhizoma Curcumae, 2 parts of Periostracum Cicadae, 3 parts of Radix Glycyrrhizae Preparata, 3 parts of Rhizoma Zingiberis, 3 parts of Fructus Toosendan, 3 parts of Rhizoma Coptis, 3 parts of Fructus Evodiae, 5 parts of Gynostemma Pentaphyllum and 5 parts of Rhizoma Curcumae. The above Chinese medicines are all granular materials with an average particle size of 0.6 mm.

[0027] A method for preparing a Chinese medicine composition for treating depression described in this embodiment comprises the following steps: S1, mixing Platycladus orientalis seeds, Rhizoma Chuanxiong, dried ginger and Rhizoma Curcumae to obtain a distilled powder, placing the distilled powder into a steam distillation apparatus for distillation, wherein the ratio of the distilled powder to distilled water is 1 g:8 L, and the distillation time is 6 h, to obtain volatile oil and a first medicinal residue; S2, mixing volatile oil: β-cyclodextrin: water in a ratio of 1 g: 8 g: 160 ml, stirring at 40° C. for 2 hours for inclusion, and freeze-drying to prepare volatile oil dry powder; S3, add bupleurum, white peony root, Albizzia julibrissin, Gynostemma pentaphyllum and the first drug residue into a first ethanol solution with a volume percentage of 70%, with a solid-liquid ratio of 1g:10ml, reflux extraction at 80°C for 2 hours, extraction times 3 times, and filter to obtain the first alcohol extraction solution and the second drug residue; S4, add coptis root, evodia rutaecarpa, toosendan fruit and turmeric into a mixed solution of 0.1 vol% HCl-60 vol% ethanol, with a solid-liquid ratio of 1g:8ml, perform ultrasonic treatment at 300W for 10min, then condense and reflux at 60°C, extract 3 times, each time for 1 hour, and obtain the second alcohol extraction solution and the third drug residue after filtration; S5, the washed and dried third medicinal residues were mixed with licorice and Gastrodia elata, and then added to water at a solid-liquid ratio of 1g:12ml, and then cellulase was added for enzymolysis, the mass of the cellulase was 0.5% of the enzymolysis reactant, the enzymolysis temperature was 40°C, the enzymolysis time was 1.5h, the cellulase activity was 200,000 IU, and the enzymolysis mixture was obtained, and dynamic countercurrent extraction was performed at 95°C for 90min, and the first water extraction solution was obtained after solid-liquid separation; S6, mixing the first alcohol extraction solution and the second alcohol extraction solution and concentrating under reduced pressure at 60° C. to a relative density of 1.1 to obtain a first concentrated solution; S7, concentrating the first aqueous solution under vacuum at 60° C. to a relative density of 1.15 to obtain a second concentrated solution; S8. After mixing the first concentrated solution and the second concentrated solution, the mixture is subjected to solvent elution using an AB-8 macroporous resin column to obtain a purified extract, which is granulated and mixed with volatile oil dry powder to obtain the traditional Chinese medicine composition.

[0028] Example 2 This embodiment provides a Chinese medicine composition for treating depression, wherein the raw materials include, by weight: 15 parts of Semen Platycladi, 7 parts of Radix Bupleuri, 5 parts of Rhizoma Gastrodiae, 5 parts of Radix Paeoniae Alba, 7 parts of Rhizoma Curcumae, 5 parts of Periostracum Cicadae, 6 parts of Radix Glycyrrhizae Preparata, 4 parts of Dry Ginger, 5 parts of Fructus Toosendan, 6 parts of Rhizoma Coptis, 5 parts of Fructus Evodiae, 7 parts of Gynostemma Pentaphyllum and 7 parts of Rhizoma Curcumae. The above Chinese medicines are all granular materials with an average particle size of 0.7 mm.

[0029] A method for preparing a Chinese medicine composition for treating depression described in this embodiment comprises the following steps: S1, mixing Platycladus orientalis seeds, Rhizoma Chuanxiong, dried ginger and Rhizoma Curcumae to obtain a distilled powder, placing the distilled powder into a steam distillation apparatus for distillation, wherein the ratio of the distilled powder to distilled water is 1 g:10 L, and the distillation time is 4 h, to obtain volatile oil and a first medicinal residue; S2, mixing volatile oil: β-cyclodextrin: water in a ratio of 1 g: 12 g: 240 ml, stirring at 60° C. for 1 hour for inclusion, and freeze-drying to prepare volatile oil dry powder; S3, taking Bupleurum, White Peony Root, Albizzia Julibrissin, Gynostemma Pentaphyllum and the first drug residue, adding them into the first ethanol solution with a volume percentage of 75%, the solid-liquid ratio is 1g:10ml, reflux extraction at 85°C, 1.5 hours, extraction times is 2 times, and filtering to obtain the first alcohol extraction solution and the second drug residue; S4, add coptis root, evodia rutaecarpa, toosendan fruit and turmeric into a mixed solution of 0.1 vol% HCl-60 vol% ethanol, with a solid-liquid ratio of 1g:10ml, perform ultrasonic treatment at 300W for 10min, condense and reflux at 65°C, extract 3 times, each time for 1 hour, and obtain the second alcohol extraction solution and the third drug residue after filtration; S5, the washed and dried third medicinal residues were mixed with Radix Glycyrrhizae Preparata and Rhizoma Gastrodiae and added into water in a ratio of 1g:15ml, and then cellulase was added for enzymolysis, the mass of cellulase was 0.7% of the enzymolysis reactant, the enzymolysis temperature was 45°C, the enzymolysis time was 1h, the cellulase activity was 200,000 IU, and the enzymolysis mixture was obtained, and dynamic countercurrent extraction was performed at 100°C for 90min, and the first water extraction solution was obtained after solid-liquid separation; S6, mixing the first alcohol extraction solution and the second alcohol extraction solution and concentrating under reduced pressure at 60° C. to a relative density of 1.1 to obtain a first concentrated solution; S7, concentrating the first aqueous solution under vacuum at 60° C. to a relative density of 1.15 to obtain a second concentrated solution; S8. After mixing the first concentrated solution and the second concentrated solution, the mixture is subjected to solvent elution using an AB-8 macroporous resin column to obtain a purified extract, which is granulated and mixed with volatile oil dry powder to obtain the traditional Chinese medicine composition.

[0030] Example 3 This embodiment provides a Chinese medicine composition for treating depression, wherein the raw materials include, by weight: 12 parts of Semen Platycladi, 6 parts of Radix Bupleuri, 4 parts of Rhizoma Gastrodiae, 4 parts of Radix Paeoniae Alba, 6 parts of Rhizoma Curcumae, 4 parts of Periostracum Cicadae, 5 parts of Radix Glycyrrhizae Preparata, 3.5 parts of Rhizoma Zingiberis, 4 parts of Fructus Toosendan, 4 parts of Rhizoma Coptis, 4 parts of Fructus Evodiae, 6 parts of Gynostemma Pentaphyllum and 6 parts of Rhizoma Curcumae. The above Chinese medicines are all granular materials with an average particle size of 0.65 mm.

[0031] A method for preparing a Chinese medicine composition for treating depression described in this embodiment comprises the following steps: S1, mixing Platycladus orientalis seeds, Rhizoma Chuanxiong, dried ginger and Rhizoma Curcumae to obtain a distilled powder, placing the distilled powder into a steam distillation apparatus for distillation, wherein the ratio of the distilled powder to distilled water is 1 g:9 L, and the distillation time is 5 h, to obtain volatile oil and a first medicinal residue; S2, mixing volatile oil: β-cyclodextrin: water in a ratio of 1 g: 10 g: 220 ml, stirring at 50° C. for 1.5 hours for inclusion, and freeze-drying to prepare volatile oil dry powder; S3, add bupleurum, white peony root, Albizzia julibrissin, Gynostemma pentaphyllum and the first drug residue into a first ethanol solution with a volume percentage of 73%, with a solid-liquid ratio of 1g:10ml, reflux extraction at 83°C for 1.6 hours, extraction times 3 times, and filter to obtain the first alcohol extraction solution and the second drug residue; S4, add coptis root, evodia rutaecarpa, toosendan fruit and turmeric into a mixed solution of 0.1 vol% HCl-60 vol% ethanol, with a solid-liquid ratio of 1g:9ml, perform ultrasonic treatment at 300W for 10min, condense and reflux at 63°C, extract 3 times, each time for 1 hour, and obtain the second alcohol extraction solution and the third drug residue after filtration; S5, the washed and dried third medicinal residues were mixed with licorice and Gastrodia elata, and then added to water at a solid-liquid ratio of 1g:13ml, and then cellulase was added for enzymolysis, the mass of the cellulase was 0.6% of the enzymolysis reactant, the enzymolysis temperature was 43°C, the enzymolysis time was 1.3h, the cellulase activity was 200,000 IU, and the enzymolysis mixture was obtained, and dynamic countercurrent extraction was performed at 96°C for 90min, and the first water extraction solution was obtained after solid-liquid separation; S6, mixing the first alcohol extraction solution and the second alcohol extraction solution and concentrating under reduced pressure at 60° C. to a relative density of 1.1 to obtain a first concentrated solution; S7, concentrating the first aqueous solution under vacuum at 60° C. to a relative density of 1.15 to obtain a second concentrated solution; S8. After mixing the first concentrated solution and the second concentrated solution, the mixture is subjected to solvent elution using an AB-8 macroporous resin column to obtain a purified extract, which is granulated and mixed with volatile oil dry powder to obtain the traditional Chinese medicine composition.

[0032] Comparative Example 1 This comparative example is similar to Example 1, except that in this comparative example, an equal amount of Albizzia julibrissin bark is replaced by Ligusticum chuanxiong.

[0033] Comparative Example 2 This comparative example is similar to Example 1, except that in this comparative example, an equal amount of Gynostemma pentaphyllum is replaced by Bupleurum chinense.

[0034] 70 healthy male rats (body weight 180-200g) were taken and divided into seven groups on average. The rats in group 1 were raised normally throughout the whole process as the blank group. The rats in groups 2, 3, 4, 5, 6 and 7 were subjected to CUMS modeling. CUMS modeling The modeling process includes fasting and water deprivation for 24 hours, lighting all night for 24 hours, swimming in cold water at 4°C for 5 minutes, swimming for 5 minutes, clamping the tail for 1 minute, horizontal shaking for 10 minutes, and moist dressing for 24 hours. During the modeling process, rats were raised normally except for the fasting and water deprivation period. After the modeling was completed, the 8 groups of rats continued to be raised normally under the same environment, and the rats in groups 3, 4, 5, 6 and 7 were gavaged with the Chinese medicine composition prepared in Examples 1, 2, 3 and Comparative Examples 1 and 2, respectively, at a dose of 80 mg / kg / day, and the administration was continued for 21 days.

[0035] The open field movement scores of rats in each group were counted before modeling, after modeling, and after continuous administration for 21 days. Open field test: Rats were placed one by one in the center of the bottom square of an uncovered cardboard box (50 cm×50 cm×50 cm) for a total of 5 minutes. The rats were adapted to the environment in the first minute, and the number of crawling squares was recorded from the second minute, i.e., the horizontal movement score. The autonomous activity and anxiety-depression behavior of rats were evaluated by analyzing the horizontal movement score. The horizontal movement scores of rats in each group are shown in Table 1.

[0036] Table 1 Horizontal movement scores of rats in each group Group Before modeling After modeling After 21 days of continuous administration 1 group 119.98±5.31 123.12±4.61 108.31±4.97 2 groups 120.9±4.02 <![CDATA[78.35±4.76 ** ]]> <![CDATA[69.3±4.60 ** ]]> 3 groups 119.43±5.25 <![CDATA[85.72±5.43 ## ]]> <![CDATA[98.05±4.91 ## ]]> 4 groups 122.77±5.88 <![CDATA[82.32±5.46 ## ]]> <![CDATA[99.22±4.8 ## ]]> 5 groups 123.46±3.46 <![CDATA[81.43±4.56 ## ]]> <![CDATA[97.61±5.33 ## ]]> 6 groups 121.16±3.22 <![CDATA[82.34±3.92 ## ]]> <![CDATA[93.19±4.62 ## ]]> 7 groups 121.24±5.85 <![CDATA[87.98±4.12 ## ]]> <![CDATA[94.33±4.91 ## ]]> Note: **Compared with the blank group, P<0.01; ##Compared with the model group, P<0.01.

[0037] Comparison of the data of group 2 with those of groups 3, 4, and 5 shows that the composition of the present invention has the effect of treating depression in rats. Comparison of the data of group 3 with those of groups 6 and 7 shows that not adding Albizzia Julibrissin and Gynostemma pentaphyllum will have an adverse effect on the effect of the composition in treating depression.

Claims

1. A Chinese medicine composition for treating depression, characterized in that: Its raw materials include: pine nuts, bupleurum, gastrodia, white peony root, turmeric, cicada shell, roasted licorice, dried ginger, toosendan fruit, coptis root, evodia rutaecarpa, julibrissin bark, ligusticum chuanxiong and gynostemma pentaphyllum.

2. A Chinese medicine composition for treating depression according to claim 1, characterized in that: The raw materials of the traditional Chinese medicine composition also include Curcuma zedoaria.

3. A Chinese medicine composition for treating depression according to claim 2, characterized in that: The raw materials include, by weight: 11-15 parts of pine nuts, 5-7 parts of bupleurum, 3-5 parts of gastrodia elata, 3-5 parts of white peony root, 5-7 parts of turmeric, 2-5 parts of cicada shells, 3-6 parts of roasted liquorice, 3-4 parts of dried ginger, 3-5 parts of toosendan fruits, 3-6 parts of coptis chinensis, 3-5 parts of evodia rutaecarpa, 5-7 parts of gynostemma pentaphyllum and 5-7 parts of zedoaria.

4. A Chinese medicine composition for treating depression according to claim 2, characterized in that: The average particle sizes of the cypress seeds, bupleurum, gastrodia, white peony root, turmeric, cicada shell, licorice root, dried ginger, toosendan fruit, coptis root, evodia rutaecarpa, julibrissin bark, ligusticum pentaphyllum, and zedoaria are all 0.6-0.7 mm.

5. A method for preparing a Chinese medicine composition for treating depression as claimed in any one of claims 1 to 4, characterized in that: The following steps are involved: S1. Mixing pine nuts, chuanxiong, dried ginger and zedoaria to obtain a distilled powder, placing the distilled powder into a distillation apparatus for distillation, wherein the ratio of the distilled powder to distilled water is 1 g: 8-10 L, to obtain volatile oil and a first medicinal residue; S2, mixing volatile oil: β-cyclodextrin: water in a ratio of 1 g: 8-12 g: 160-240 ml, stirring and including, and freeze-drying to prepare volatile oil dry powder; S3, adding bupleurum root, white peony root, Albizzia julibrissin, Gynostemma pentaphyllum and the first medicinal residue to the first ethanol solution, extracting under reflux at 80-85° C., and filtering to obtain the first alcohol extraction solution and the second medicinal residue; S4, adding coptis root, evodia rutaecarpa, toosendan fruit and turmeric to the second ethanol solution, condensing and refluxing at 60-65° C. after ultrasonication, and filtering to obtain the second ethanol extraction solution and the third medicinal residue; S5, mixing the washed and dried third medicinal residue with roasted licorice and Gastrodia elata, adding the mixture into water at a solid-liquid ratio of 1 g:12-15 ml, adding cellulase for enzymolysis, performing dynamic countercurrent extraction at 95-100° C., and obtaining a first aqueous extract solution after solid-liquid separation; S6, mixing the first alcohol extraction solution and the second alcohol extraction solution and concentrating under reduced pressure to obtain a first concentrated solution; S7, concentrating the first aqueous solution under vacuum to obtain a second concentrated solution; S8, mixing the first concentrated solution and the second concentrated solution, eluting the mixture, and then granulating the mixture and mixing it with volatile oil dry powder to obtain the traditional Chinese medicine composition.

6. The preparation method according to claim 5, characterized in that: The solid-liquid ratio during the reflux extraction process in S3 is 1 g:10 ml.

7. The preparation method according to claim 5, characterized in that: The total mass of the coptis root, evodia rutaecarpa, toosendan fruit and turmeric described in S4 and the second ethanol solution has a solid-liquid ratio of 1g:8-10ml.

8. The preparation method according to claim 5, characterized in that: The second ethanol solution in S4 is a mixed solution of 0.1 vol% HCl - 60 vol% ethanol.

9. The preparation method according to claim 5, characterized in that: The mass of the cellulase in S5 is 0.5%-0.7% of the enzymatic hydrolysis reactant, the enzymatic hydrolysis temperature is 40-45° C., and the enzymatic hydrolysis time is 1-1.5 h.

10. A pill for treating depression prepared based on the Chinese medicine composition according to claims 1 to 4.