Application of carrageenan-containing composition in preparation of wound dressing

Abstract

The invention relates to the technical field of biological medicines, and particularly discloses application of a carrageenan-containing composition in preparation of a wound dressing. The carrageenan-containing composition is prepared from carrageenan and a traditional Chinese medicine extract, wherein the mass ratio of the carrageenan to the traditional Chinese medicine extract is (5-10): (1-5); the traditional Chinese medicine extract is obtained by extracting traditional Chinese medicine ampelopsis japonica and angelica sinensis as raw materials. Researches show that the composition obtained by combining the carrageenan and extracts extracted from the traditional Chinese medicines radix ampelopsis and radix angelicae sinensis as raw materials has a relatively good effect of promoting wound healing; therefore, when the carrageenan-containing composition is used as a material for preparing wound excipients, the carrageenan-containing composition has important application value for promoting wound healing.

Description

Technical Field

[0001] The present invention relates to the technical field of biomedicine, and in particular to application of a composition containing carrageenan in preparing a wound dressing. Background Art

[0002] Traditional wound dressings mainly include basic types such as dry gauze and cotton pads. Although they can provide certain protection, their functions are relatively simple. For example, dry gauze is easy to adhere to the new granulation tissue, which may cause secondary damage when replaced; and in some cases, they cannot effectively prevent bacterial invasion or maintain a moist environment in the wound, which is not conducive to the migration and regeneration of epithelial cells.

[0003] Carrageenan, a natural polysaccharide derived from marine plants, can be divided into three main types according to its chemical structure: κ-type, ι-type and λ-type. Among them, κ-carrageenan is the most common. It can form a strong gel structure at a low concentration and exhibits excellent mechanical strength and stability. This unique physical property makes it very suitable for the preparation of various biomedical materials. In addition to its excellent physical properties, carrageenan also exhibits a variety of biological activities. Studies have shown that carrageenan has significant antibacterial effects, especially a strong inhibitory effect on Gram-positive bacteria; in addition, it can reduce inflammatory responses by regulating immune responses, and stimulate the proliferation of fibroblasts and keratinocytes to promote wound healing. These characteristics make carrageenan a highly potential raw material for wound dressings. However, the effect of carrageenan alone in promoting wound healing is limited and needs to be further improved. Summary of the invention

[0004] In order to overcome at least one of the technical problems existing in the prior art, the present invention provides a use of a composition containing carrageenan in preparing a wound dressing.

[0005] The above technical problems to be solved by the present invention are achieved through the following technical solutions:

[0006] The present invention first provides a carrageenan-containing composition, which comprises carrageenan and a traditional Chinese medicine extract; wherein the mass ratio of carrageenan to the traditional Chinese medicine extract is 5-10:1-5;

[0007] The Chinese medicine extract is obtained by extracting Chinese medicines Bletilla striata and Angelica sinensis as raw materials.

[0008] The invention provides a new carrageenan-containing composition; studies have shown that the composition obtained by combining carrageenan with extracts extracted from Chinese medicinal herbs such as Ampelopsis pilosula and Angelica sinensis has a good effect of promoting wound healing.

[0009] Preferably, the mass ratio of carrageenan to the traditional Chinese medicine extract is 7:3.

[0010] Preferably, the Chinese herbal medicine extract is prepared by the following method:

[0011] The Chinese medicinal materials Bletilla striata and Angelica sinensis are mixed to obtain a mixed raw material, which is then extracted with an organic solvent. After the extraction, the extract is taken, concentrated and dried to obtain a Bletilla striata-Angelica sinensis solvent extract; the Bletilla striata-Angelica sinensis solvent extract is taken to obtain the Chinese medicinal extract.

[0012] Preferably, the mass ratio of Bletilla striata to Angelica sinensis is 1-3:1-3.

[0013] Most preferably, the mass ratio of Bletilla striata to Angelica sinensis is 1:1.

[0014] Preferably, the organic solvent is selected from one or a mixture of more than one of ethanol, methanol, acetone, ether and chloroform.

[0015] Preferably, the organic solvent consists of methanol, acetone and ether.

[0016] The inventors found in further research that in the process of preparing Chinese herbal medicine extracts using Bletilla striata and Angelica sinensis as raw materials, the selection of organic solvents is very critical; when the Chinese herbal medicine extracts are extracted using an organic solvent composed of methanol, acetone and ether, their wound healing-promoting effect is much higher than that of the Chinese herbal medicine extracts extracted using a single methanol, acetone or ether as an organic solvent; the use of an organic solvent composed of methanol, acetone and ether can synergistically improve the wound healing-promoting effect of the extracted Chinese herbal medicine extracts.

[0017] Preferably, the volume ratio of methanol, acetone and diethyl ether is 3-6:1-3:1-3.

[0018] Most preferably, the volume ratio of methanol, acetone and diethyl ether is 5:2:2.

[0019] Preferably, the ratio of the mixed raw material to the organic solvent is 1 kg:10-30 L.

[0020] Most preferably, the ratio of the mixed raw material to the organic solvent is 1 kg:20 L.

[0021] Preferably, the extraction is immersion extraction; the immersion extraction time is 1 to 3 days and the temperature is 20 to 30°C.

[0022] Most preferably, the extraction is immersion extraction; the immersion extraction time is 2 days and the temperature is 25°C.

[0023] The present invention also provides an application of the carrageenan-containing composition in preparing wound dressings.

[0024] Beneficial effects: The present invention provides a novel carrageenan-containing composition; studies have shown that a composition obtained by combining carrageenan with extracts obtained by extracting Chinese medicinal materials such as Ampelopsis pilosula and Angelica sinensis as raw materials has a good effect on promoting wound healing; therefore, using the carrageenan-containing composition of the present invention as a material for preparing wound dressings has important application value for promoting wound healing. DETAILED DESCRIPTION

[0025] The present invention is further explained below in conjunction with specific examples, but the examples do not limit the present invention in any form.

[0026] Example 1 Preparation of a composition containing carrageenan

[0027] The kappa-carrageenan and the Chinese herbal medicine extract are mixed uniformly in a mass ratio of 7:3 to obtain the carrageenan-containing composition;

[0028] The Chinese medicine extract is prepared by the following method:

[0029] The Chinese medicinal materials of Radix Bletillae Sinensis and Radix Angelicae Sinensis are mixed in a mass ratio of 1:1 to obtain a mixed raw material, and then an organic solvent is used for immersion extraction at 25°C for 2 days. After the extraction is completed, the extract is filtered to obtain the extract, and the extract is concentrated and dried to obtain the Radix Bletillae Sinensis-Radix Angelicae Sinensis solvent extract; the Radix Bletillae Sinensis-Radix Angelicae Sinensis solvent extract is obtained to obtain the Chinese medicinal extract;

[0030] The organic solvent is methanol; wherein the usage ratio of the mixed raw material to the organic solvent is 1kg:20L.

[0031] Example 2 Preparation of a composition containing carrageenan

[0032] The kappa-carrageenan and the Chinese herbal medicine extract are mixed uniformly in a mass ratio of 7:3 to obtain the carrageenan-containing composition;

[0033] The Chinese medicine extract is prepared by the following method:

[0034] The Chinese medicinal materials of Radix Bletillae Sinensis and Radix Angelicae Sinensis are mixed in a mass ratio of 1:1 to obtain a mixed raw material, and then an organic solvent is used for immersion extraction at 25°C for 2 days. After the extraction is completed, the extract is filtered to obtain the extract, and the extract is concentrated and dried to obtain the Radix Bletillae Sinensis-Radix Angelicae Sinensis solvent extract; the Radix Bletillae Sinensis-Radix Angelicae Sinensis solvent extract is obtained to obtain the Chinese medicinal extract;

[0035] The organic solvent is acetone; wherein the usage ratio of the mixed raw material to the organic solvent is 1kg:20L.

[0036] Example 3 Preparation of a composition containing carrageenan

[0037] The kappa-carrageenan and the Chinese herbal medicine extract are mixed uniformly in a mass ratio of 7:3 to obtain the carrageenan-containing composition;

[0038] The Chinese medicine extract is prepared by the following method:

[0039] The Chinese medicinal materials of Radix Bletillae Sinensis and Radix Angelicae Sinensis are mixed in a mass ratio of 1:1 to obtain a mixed raw material, and then an organic solvent is used for immersion extraction at 25°C for 2 days. After the extraction is completed, the extract is filtered to obtain the extract, and the extract is concentrated and dried to obtain the Radix Bletillae Sinensis-Radix Angelicae Sinensis solvent extract; the Radix Bletillae Sinensis-Radix Angelicae Sinensis solvent extract is obtained to obtain the Chinese medicinal extract;

[0040] The organic solvent is ether; wherein the usage ratio of the mixed raw material to the organic solvent is 1kg:20L.

[0041] Example 4 Preparation of a composition containing carrageenan

[0042] The kappa-carrageenan and the Chinese herbal medicine extract are mixed evenly in a mass ratio of 7:3 to obtain the carrageenan-containing composition;

[0043] The Chinese medicine extract is prepared by the following method:

[0044] The Chinese medicinal materials of Radix Bletillae Sinensis and Radix Angelicae Sinensis are mixed in a mass ratio of 1:1 to obtain a mixed raw material, and then an organic solvent is used for immersion extraction at 25°C for 2 days. After the extraction is completed, the extract is filtered to obtain the extract, and the extract is concentrated and dried to obtain the Radix Bletillae Sinensis-Radix Angelicae Sinensis solvent extract; the Radix Bletillae Sinensis-Radix Angelicae Sinensis solvent extract is obtained to obtain the Chinese medicinal extract;

[0045] The organic solvent is ethanol with a volume fraction of 95%; wherein the usage ratio of the mixed raw material to the organic solvent is 1kg:20L.

[0046] Example 5 Preparation of a composition containing carrageenan

[0047] The kappa-carrageenan and the Chinese herbal medicine extract are mixed evenly in a mass ratio of 7:3 to obtain the carrageenan-containing composition;

[0048] The Chinese medicine extract is prepared by the following method:

[0049] The Chinese medicinal materials of Radix Bletillae Sinensis and Radix Angelicae Sinensis are mixed in a mass ratio of 1:1 to obtain a mixed raw material, and then an organic solvent is used for immersion extraction at 25°C for 2 days. After the extraction is completed, the extract is filtered to obtain the extract, and the extract is concentrated and dried to obtain the Radix Bletillae Sinensis-Radix Angelicae Sinensis solvent extract; the Radix Bletillae Sinensis-Radix Angelicae Sinensis solvent extract is obtained to obtain the Chinese medicinal extract;

[0050] The organic solvent is chloroform; wherein the usage ratio of the mixed raw material to the organic solvent is 1kg:20L.

[0051] Example 6 Preparation of a composition containing carrageenan

[0052] The kappa-carrageenan and the Chinese herbal medicine extract are mixed uniformly in a mass ratio of 7:3 to obtain the carrageenan-containing composition;

[0053] The Chinese medicine extract is prepared by the following method:

[0054] The Chinese medicinal materials of Radix Bletillae Sinensis and Radix Angelicae Sinensis are mixed in a mass ratio of 1:1 to obtain a mixed raw material, and then an organic solvent is used for immersion extraction at 25°C for 2 days. After the extraction is completed, the extract is filtered to obtain the extract, and the extract is concentrated and dried to obtain the Radix Bletillae Sinensis-Radix Angelicae Sinensis solvent extract; the Radix Bletillae Sinensis-Radix Angelicae Sinensis solvent extract is obtained to obtain the Chinese medicinal extract;

[0055] The organic solvent is composed of methanol, acetone and ether in a volume ratio of 5:2:2; wherein the dosage ratio of the mixed raw material to the organic solvent is 1kg:20L.

[0056] Example 7 Preparation of a composition containing carrageenan

[0057] The kappa-carrageenan and the Chinese herbal medicine extract are mixed uniformly in a mass ratio of 7:3 to obtain the carrageenan-containing composition;

[0058] The Chinese medicine extract is prepared by the following method:

[0059] The Chinese medicinal materials of Radix Bletillae Sinensis and Radix Angelicae Sinensis are mixed in a mass ratio of 1:1 to obtain a mixed raw material, and then an organic solvent is used for immersion extraction at 25°C for 2 days. After the extraction is completed, the extract is filtered to obtain the extract, and the extract is concentrated and dried to obtain the Radix Bletillae Sinensis-Radix Angelicae Sinensis solvent extract; the Radix Bletillae Sinensis-Radix Angelicae Sinensis solvent extract is obtained to obtain the Chinese medicinal extract;

[0060] The organic solvent is composed of methanol and ether in a volume ratio of 5:2; wherein the usage ratio of the mixed raw material to the organic solvent is 1kg:20L.

[0061] Example 8 Preparation of a composition containing carrageenan

[0062] The kappa-carrageenan and the Chinese herbal medicine extract are mixed uniformly in a mass ratio of 7:3 to obtain the carrageenan-containing composition;

[0063] The Chinese medicine extract is prepared by the following method:

[0064] The Chinese medicinal materials of Radix Bletillae Sinensis and Radix Angelicae Sinensis are mixed in a mass ratio of 1:1 to obtain a mixed raw material, and then an organic solvent is used for immersion extraction at 25°C for 2 days. After the extraction is completed, the extract is filtered to obtain the extract, and the extract is concentrated and dried to obtain the Radix Bletillae Sinensis-Radix Angelicae Sinensis solvent extract; the Radix Bletillae Sinensis-Radix Angelicae Sinensis solvent extract is obtained to obtain the Chinese medicinal extract;

[0065] The organic solvent is composed of methanol and acetone in a volume ratio of 5:2; wherein the usage ratio of the mixed raw material to the organic solvent is 1kg:20L.

[0066] Experimental Example 1: Skin healing promotion experiment

[0067] The carrageenan-containing compositions prepared in Examples 1 to 8 were swollen with 20 times the weight of water and used as dressings 1 to 8. Meanwhile, κ-carrageenan was swollen with 20 times the weight of water and used as a control dressing.

[0068] A male Kunming mouse weighing 25+1 g was taken, and the hair on the back was cut after anesthesia, and then a depilatory cream was used for hair removal; after hair removal, a circular skin with a diameter of 10 mm was cut on the back of the mouse to establish a skin injury model mouse.

[0069] 90 mice with skin injury model established according to the above method were randomly divided into 9 groups, with 10 mice in each group; the experimental groups 1 to 8 were applied with dressings 1 to 8, respectively; the control group was applied with control dressing; the average healing time of the skin of each group of mice was recorded, and the results are shown in Table 1.

[0070] Table 1. Results of skin healing promotion experiment

[0071]

[0072]

[0073] It can be seen from the experimental results in Table 1 that the carrageenan-containing compositions prepared in Examples 1 to 5 can significantly shorten the average healing time of mouse skin compared to κ-carrageenan alone; this indicates that the composition obtained by combining carrageenan with extracts extracted from the Chinese medicinal materials Bletilla striata and Angelica sinensis as raw materials can significantly improve the wound healing effect compared to carrageenan.

[0074] It can be seen from the experimental results in Table 1 that the carrageenan-containing composition prepared in Example 6 can further significantly shorten the average healing time of mouse skin compared with the carrageenan-containing composition prepared in Examples 1 to 5. This shows that in the process of preparing Chinese medicine extracts using Chinese medicines Bletilla striata and Angelica sinensis as raw materials, the selection of organic solvents is very critical. When the Chinese medicine extracts extracted with an organic solvent composed of methanol, acetone and ether have a significantly higher effect on promoting wound healing than the Chinese medicine extracts extracted with a single methanol, acetone or ether as an organic solvent, and at the same time, the effect of promoting wound healing is significantly higher than the Chinese medicine extracts extracted with other organic solvents. The use of an organic solvent composed of methanol, acetone and ether can synergistically improve the wound healing effect of the extracted Chinese medicine extracts.

[0075] It can be seen from the experimental results in Table 1 that the carrageenan-containing compositions prepared in Examples 7 and 8 did not further significantly shorten the average healing time of mouse skin compared with the carrageenan-containing compositions prepared in Examples 1 to 5. This shows that only the Chinese herbal extracts extracted with organic solvents consisting of methanol, acetone and ether can have a significantly higher wound healing effect than the Chinese herbal extracts extracted with single methanol, acetone or ether as organic solvents, and can synergistically improve the wound healing effect of the extracted Chinese herbal extracts.

Claims

1. A composition containing carrageenan, characterized in that: Comprising carrageenan and a Chinese herbal medicine extract; wherein the mass ratio of carrageenan to the Chinese herbal medicine extract is 5-10:1-5; The Chinese medicine extract is obtained by extracting Chinese medicines Bletilla striata and Angelica sinensis as raw materials.

2. The carrageenan-containing composition according to claim 1, characterized in that The mass ratio of carrageenan to Chinese herbal medicine extract is 7:

3.

3. The carrageenan-containing composition according to claim 1, characterized in that: The Chinese medicine extract is prepared by the following method: The Chinese medicinal materials Bletilla striata and Angelica sinensis are mixed to obtain a mixed raw material, which is then extracted with an organic solvent. After the extraction, the extract is taken, concentrated and dried to obtain a Bletilla striata-Angelica sinensis solvent extract; the Bletilla striata-Angelica sinensis solvent extract is taken to obtain the Chinese medicinal extract.

4. The carrageenan-containing composition according to claim 3, characterized in that: The mass ratio of Bletilla striata to Angelica sinensis is 1-3:1-3; Most preferably, the mass ratio of Bletilla striata to Angelica sinensis is 1:

1.

5. The carrageenan-containing composition according to claim 3, characterized in that: The organic solvent is selected from one or a mixture of ethanol, methanol, acetone, ether and chloroform.

6. The carrageenan-containing composition according to claim 3, characterized in that: The organic solvent consists of methanol, acetone and ether.

7. The carrageenan-containing composition according to claim 6, characterized in that: The volume ratio of methanol, acetone and ether is 3-6:1-3:1-3; Most preferably, the volume ratio of methanol, acetone and diethyl ether is 5:2:

2.

8. The carrageenan-containing composition according to claim 3, characterized in that: The ratio of the mixed raw material to the organic solvent is 1kg:10-30L; Most preferably, the ratio of the mixed raw material to the organic solvent is 1 kg:20 L.

9. The carrageenan-containing composition according to claim 3, characterized in that: The extraction is immersion extraction; the immersion extraction time is 1 to 3 days, and the temperature is 20 to 30°C; Most preferably, the extraction is immersion extraction; the immersion extraction time is 2 days and the temperature is 25°C.

10. Use of the carrageenan-containing composition according to any one of claims 1 to 9 in the preparation of wound dressings.

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