Novel filler for purifying aquaculture water and preparation process of novel filler

Abstract

The novel filler comprises an aerobic filler A, an anoxic filler B, autotrophic nitrifying bacteria, autotrophic denitrifying bacteria and water, the aerobic filler A comprises a particle mixture and volcanic rock particles, and the particle mixture comprises diatomite, vermiculite powder, bentonite, activated clay and wollastonite. The formula comprises potassium feldspar powder, dolomite powder, zeolite powder, manganese sand particles, quartz sand particles and a coagulant; the anoxic filler B comprises calcium-magnesium-based diatomite, expanded vermiculite powder, calcium carbonate powder and elemental sulfur powder. According to the novel filler for purifying the aquaculture water body and the preparation process of the novel filler, ammonia nitrogen, nitrite and nitrate nitrogen in the water body can be removed at the same time, a proper habitat environment can be provided for autotrophic nitrifying bacteria and sulfur autotrophic denitrifying bacteria through the characteristics of the filler, and under the condition that an additional carbon source is not needed, the novel filler can be used for purifying the aquaculture water body. Ammonia nitrogen, nitrate nitrogen and nitrite nitrogen in water can be effectively removed.

Description

Technical Field

[0001] The invention belongs to the technical field of water purification, and in particular relates to a new filler for purifying aquaculture water and a preparation process thereof. Background Art

[0002] The nitrogen in aquaculture water mainly comes from leftover bait, metabolic products of farmed animals, and remains of aquatic plants and animals. According to research reports, 60% to 70% of the nitrogen in feed is excreted into the water. Excessive nitrogen content in aquaculture will bring the following hazards:

[0003] (1) Toxic effects of ammonia nitrogen. Increased ammonia nitrogen in water bodies will inhibit the excretion of ammonia in fish, increase the concentration of ammonia in the blood and tissues, reduce the oxygen carrying capacity of the blood, and increase blood CO. 2 The concentration increased; NH 3 It is uncharged, has high fat solubility, and can easily pass through the cell membrane to directly cause fish poisoning, causing breathing difficulties, increased secretions, and exhaustion and death of fish; NH 3 It will cause damage to the fish's gill epidermal cells and reduce the fish's immunity.

[0004] (2) Toxic effects of nitrite nitrogen. High concentrations of NO 2 It will cause the hemoglobin (reduced hemoglobin) containing divalent iron in the fish's blood to turn into methemoglobin containing trivalent iron, thereby affecting the blood's function of carrying oxygen, causing tissue hypoxia, deteriorating the physical fitness of the fish and even affecting their growth, creating conditions for the invasion of pathogens.

[0005] (3) Hazards of nitrate nitrogen. Excessive nitrate nitrogen content will lead to eutrophication of water bodies and affect the health of fish and shrimp. Excessive nitrate nitrogen will cause local short-range denitrification in the water environment, which will lead to increased nitrite. This will affect the health of fish and shrimp. The main substances in water that are harmful to fish are nitrogen and nitrite. my country's aquaculture water quality standards stipulate that nitrogen is less than 0.5 mg / L and nitrite is less than 0.2 mg / L.

[0006] Although traditional chemical treatment methods can effectively reduce the concentration of harmful substances in water, long-term use will destroy the ecological balance of water quality and cause potential harm to aquatic organisms. At the same time, the continuous investment of chemical drugs also increases operating costs. Biological methods have lower cost investment, heterotrophic microorganisms require external carbon sources, which are less economical. At the same time, external carbon sources may cultivate pathogenic bacteria, posing a threat to the survival of aquatic animals, and traditional biological methods can usually only solve single or two indicators among ammonia nitrogen, nitrate nitrogen, and salt.

[0007] Therefore, how to remove ammonia nitrogen, nitrate nitrogen and hypochlorite from water at the same time without additional carbon source becomes the key technical problem to be solved in this plan. Summary of the invention

[0008] The purpose of the present invention is to provide a new filler for purifying aquaculture water and a preparation process thereof, which solves the technical problem of simultaneously removing ammonia nitrogen, nitrite nitrogen and nitrate nitrogen in water without an additional carbon source. The filler itself can provide a suitable habitat for autotrophic nitrifying bacteria and sulfur autotrophic denitrifying bacteria, and can effectively remove ammonia nitrogen, nitrate nitrogen and nitrite nitrogen in water to maintain water cleanliness and ecological balance. The filler is suitable for water purification in aquaculture water.

[0009] A novel filler for purifying aquaculture water, comprising aerobic filler A, anoxic filler B, autotrophic nitrifying bacteria, autotrophic denitrifying bacteria, and water, wherein the aerobic filler A comprises a particle mixture and volcanic rock particles, and the particle mixture comprises diatomaceous earth, vermiculite powder, bentonite, activated clay, wollastonite, potassium feldspar powder, dolomite powder, zeolite powder, manganese sand and gravel particles, quartz sand particles, and a coagulant;

[0010] The oxygen-deficient filler B includes calcium-magnesium-based diatomaceous earth, expanded vermiculite powder, calcium carbonate powder, and elemental sulfur powder;

[0011] The mass ratio of the aerobic filler A to the anoxic filler B is (1-3): (1.5-3).

[0012] The content of each component in the particle mixture is as follows by mass: 30-60g diatomaceous earth, 30-60g vermiculite powder, 20-40g bentonite, 20-40g activated clay, 10-30g wollastonite, 20-40g potassium feldspar powder, 10-30g dolomite powder, 10-30g zeolite powder, 10-20g manganese sand and gravel particles, and 50-60g quartz sand particles. The coagulant is 20-50g foamed magnesite cement.

[0013] In terms of mass, the mass ratio of the particle mixture to the volcanic rock particles is 1:0.5-3, and the content of autotrophic nitrifying bacteria is 1000ppm / 1kg aerobic filler A, so that the content of sodium nitrate and sodium nitrite is between 80-200ppm; the particle size of the volcanic rock particles is 1.5-3cm.

[0014] In terms of mass fraction, (diatomaceous earth + vermiculite powder) / bentonite = 0.5-3;

[0015] (Activated clay + wollastonite) / bentonite = 0.5-3;

[0016] (potassium feldspar powder + dolomite powder + zeolite powder + manganese sand and stone particles + quartz sand particles) / bentonite = 0.5-10;

[0017] Total weight of foamed magnesia cement / particle mixture = 10%-20%.

[0018] The contents of the various components in the anoxic filler B are as follows by mass: 40-50 g of calcium-magnesium-based diatomaceous earth, 40-50 g of expanded vermiculite powder, 50-100 g of calcium carbonate powder, and 50-100 g of elemental sulfur powder.

[0019] The content of the autotrophic denitrifying bacteria is 1000 ppm / 1 kg of aerobic filler B by mass, so that the content of sodium nitrate and sodium nitrite is between 80-200 ppm.

[0020] By mass, (calcium magnesium based diatomaceous earth + expanded vermiculite powder + calcium carbonate powder) / elemental sulfur powder = 1-5.

[0021] A preparation process of a new filler for aquaculture water purification, specifically comprising the following steps:

[0022] Step S1: Mix the various components in the particle mixture, add 10-30 g of water, and press into spherical particles with a particle size of 1.5-3 cm;

[0023] Step S2: mixing the spherical particles in step S1 with volcanic rock particles at a mass ratio of 1:0.5-3 to form aerobic filler A;

[0024] Step S3: immersing the aerobic filler A in step S2 into water, adding about 1000 ppm of autotrophic nitrifying bacteria per 1 kg of filler, maintaining the content of sodium nitrate and sodium nitrite between 80-200 ppm, until a biofilm is formed on the surface of the filler;

[0025] Step S4: storing the material obtained in step S3 in a cold storage at 0-4°C to obtain a finished product;

[0026] Step S5: Mix the various components in the oxygen-deficient filler B evenly, and heat them in a sandwich pot to between 115-120° C. until the elemental sulfur in the raw material is fully dissolved and evenly mixed with other ingredients;

[0027] Step S6: granulating the material in step S5 into particles with a particle size of 1-5 cm, and cooling them for later use;

[0028] Step S7: immersing the granular filler obtained in step S6 in water, adding about 1000 ppm of autotrophic denitrifying bacteria per 1 kg of filler, maintaining the content of sodium nitrate and sodium nitrite between 80-200 ppm, until a biofilm is formed on the surface of the filler;

[0029] Step S8: The material in step S7 is placed in a cold storage at 0-4°C to obtain a finished product.

[0030] Step S9: Finally, the finished product obtained in step S4 is fully mixed with the finished product obtained in step S8 in a mass ratio of (1-2): (1.5-3) to obtain a new filler.

[0031] The mechanism of action of autotrophic nitrifying bacteria in the biofilm method in this scheme is as follows:

[0032] Nitrosation reaction: NH 4 +1.5O 2 →NO 2 - +2H + +H 2 O;

[0033] Nitration reaction: NO 2 -+0.5O 2 →NO 3 - ;

[0034] Total reaction: NH 4 + +2O 2 →NO 3 - +2H + +H 2 0;

[0035] The overall reaction equation for nitrification and cell biomass generation can also be written as:

[0036] Nitrosation reaction: 55NH 4 ++5CO 2 +76O 2 →C 5 H 2 NO 2 +54NO 2 -+52H 2 O+109H + ;

[0037] Nitration reaction: 400NO 2 - +5CO 2 +NH 4 + +195O 2 +2H 2 0→C 5 H 7 NO 2 +400NO 3 - +H + ;

[0038] Total reaction: NH 4 + +1.83O 2 +1.97HCO 3 - →0.0244C5 H 7 NO 2 +0.976NO 3 - +2.90H 2 0

[0039] +1.86CO 2 ;

[0040] According to the above chemical formula, every 1g of ammonia converted into nitrate nitrogen consumes 4.1g of dissolved oxygen and 7.05g of alkalinity (1.69g of inorganic carbon), and produces 0.20g of microbial biomass (0.105g of organic carbon) and 5.85g of CO. 2 (1.59 g inorganic carbon).

[0041] The working principle of autotrophic denitrifying bacteria in this scheme is as follows:

[0042] 10NO 3 - +11S+4.1HCO 3 - +0.5CO 2 +1.71NH 4 + +2.54H 2 O→0.92C 5 H 7 NO 2 +11SO 42 - +

[0043] 5.4N 2 +962H + ;

[0044] According to the above chemical formula, for every 1g of nitrate nitrogen converted into nitrogen, 1.97g of alkalinity (calculated as carbonate) and 2.51g of sulfur will be consumed, and N 2 .

[0045] Through the autotrophic nitrifying bacteria and autotrophic denitrifying bacteria formed on the filler, ammonia nitrogen, nitrite nitrogen and nitrate nitrogen are removed in aerobic and anoxic environments and finally converted into nitrogen gas.

[0046] The above chemical reaction formula provided in this scheme is an introduction to the basic principle, and the various filler components added provide trace elements, and each trace element can be slowly released into the water body after being made into a filler, continuously providing nutrients for functional bacteria. In addition to their own functions, the bentonite, vermiculite powder and other components, diatomaceous earth, vermiculite powder, bentonite, activated white clay, wollastonite, potassium feldspar powder, dolomite powder, zeolite powder also have a high specific surface area, and their porous structure is conducive to the attachment and colonization of microorganisms; calcium carbonate provides an inorganic carbon source to balance the pH; elemental sulfur provides a sulfur source.

[0047] In the step S3, after adding the autotrophic nitrifying bacteria, the mixed solution is subjected to electric pulse treatment, the electric pulse intensity is 5-10V / cm, and the pulse width is 20 microseconds to 40 microseconds.

[0048] In the step S7, after adding the autotrophic denitrifying bacteria, the mixed solution is subjected to electric pulse treatment, the electric pulse intensity is 8-12V / cm, and the pulse intensity is 15 microseconds to 25 microseconds.

[0049] The present invention achieves the following significant effects:

[0050] (1) The aerobic filler A and the anoxic filler B in this scheme act synergistically. The aerobic filler A is mainly used to cultivate autotrophic nitrifying bacteria. The bacteria need to use the filler and the nutrients in the water body to undergo nitrification under aerobic conditions, and can convert ammonia nitrogen in the water body into nitrite, and then into nitrate. The anoxic filler B is mainly used to cultivate autotrophic denitrifying bacteria. The bacteria need to use the filler and the nutrients in the water body to undergo denitrification under anoxic conditions, and can convert nitrate and nitrite in the water body into nitrogen gas for emission. Two conditions (aerobic and anoxic) are created by the two fillers to remove ammonia nitrogen, nitrite nitrogen, and nitrate nitrogen.

[0051] (2) Natural diatomaceous earth is fine, loose, light, porous, and has stronger water absorption and permeability, which can reach 19-65m 2 / g provides more specific surface area, thereby increasing the amount of microbial attachment and improving the autotrophic denitrification rate.

[0052] (3) Calcium magnesium-based bentonite has strong cation exchange and water absorption and expansion properties, which can buffer the Ph value, especially the ion exchange of hydrogen ions produced during sulfur autotrophic and autotrophic nitrification, thereby balancing the Ph; water absorption and expansion properties increase the hydrophilicity of the filter material surface, which is more conducive to the attachment of microorganisms.

[0053] (4) Expanded vermiculite powder has a high cation exchange capacity and cation adsorption capacity, and can absorb harmful substances in water. The slow release of nitrogen, phosphorus, potassium, aluminum, iron, magnesium, silicon and other elements can provide trace elements for the growth of sanitary organisms; calcium carbonate and magnesium carbonate provide inorganic carbon sources for microbial growth and can neutralize hydrogen ions produced during nitrification and denitrification; elemental sulfur provides electron donors for sulfur autotrophic denitrification.

[0054] (5) The most important innovation of this scheme that is different from the existing technology is that: under normal circumstances, the method used to remove ammonia nitrogen and nitrite in aquaculture water is autotrophic nitrifying bacteria or autotrophic nitrifying bacteria + carrier method. This scheme creatively adopts the method of autotrophic nitrifying bacteria + autotrophic denitrifying bacteria, which can remove both ammonia nitrogen and nitrite and nitrate nitrogen.

[0055] (6) This solution adds electric pulse treatment to the preparation process, achieving the following technical effects:

[0056] First, the electric pulse treatment enhances the activity of microorganisms on the aerobic filler, promoting their growth and reproduction, which helps to improve the efficiency of wastewater treatment;

[0057] The two-electric pulse treatment helps to change the surface characteristics of the filler, increase the oxygen transmission rate, and thus enhance the effect of aerobic reaction;

[0058] Third, the electric pulse treatment changes the microstructure of the filler, improving its strength and toughness;

[0059] Fourth, electric pulse treatment accelerates the chemical reaction in the filler and improves its performance. BRIEF DESCRIPTION OF THE DRAWINGS

[0060] Figure 1 The figure is a curve diagram of the purification effect of the carrier prepared in Example 1 in a freshwater fish tank.

[0061] Figure 2 The figure is a curve diagram of the purification effect of the carrier prepared in Example 1 in a seawater fish tank.

[0062] Figure 3 This is a curve diagram of the purification effect of the carrier prepared in Example 1 in a South American shrimp farm.

[0063] Figure 4 This is a curve diagram of the purification effect of the carrier prepared in Example 1 in a freshwater grouper farm.

[0064] Figure 5 This is a curve diagram of the purification effect of the carrier prepared in Example 1 in a koi breeding farm. DETAILED DESCRIPTION

[0065] In order to more clearly illustrate the technical features of this solution, this solution is described below through specific implementation methods.

[0066] Example 1

[0067] See also Figure 1 , the freshwater fish tank is filled with fresh water, the breeding density is 2.5kg / 100L, and the water temperature is 25-28℃.

[0068] Step 1: Put filler A (the filler includes: 30g diatomaceous earth, 30g vermiculite powder, 20g bentonite, 20g activated clay, 10g wollastonite, 20g potassium feldspar powder, 10g dolomite powder, 10g zeolite powder, 10g manganese sand and gravel particles, 50g quartz sand particles, and coagulant (50g foamed magnesia cement) into the first 2 boxes of the 4-box filter of the aquarium fish tank, and install an aeration device at the bottom of the filter.

[0069] Step 2: Place filler B (40g of calcium-magnesium-based diatomaceous earth, 40g of expanded vermiculite powder, 50g of calcium carbonate powder, and 50g of elemental sulfur powder) into the last 2 boxes of the 4-box filter of the aquarium fish tank.

[0070] Step 3: Connect the configured aquarium fish tank filter to the water in the freshwater fish tank for circulating filtration results.

[0071] At this time, for filler A, in terms of mass fraction, (diatomaceous earth + vermiculite powder) / bentonite = 3;

[0072] (Activated clay + wollastonite) / bentonite = 1.5;

[0073] (potassium feldspar powder + dolomite powder + zeolite powder + manganese sand and stone particles + quartz sand particles) / bentonite = 5;

[0074] Total weight of foamed magnesia cement / particle mixture = 19.2%.

[0075] For filler B, by mass, (calcium magnesium-based diatomaceous earth+expanded vermiculite powder+calcium carbonate powder) / elemental sulfur powder=2.6.

[0076] The mass ratio of the aerobic filler A to the anoxic filler B is 1.44.

[0077]

[0078] like Figure 1 As shown in Table 1, in the experimental group using fillers in Example 1, ammonia nitrogen can be removed by 96% within 1 week and maintained at a low level; nitrite nitrogen can be completely removed within 2 days and has been stable near 0, the effect is far better than the blank group without fillers. Nitrate nitrogen can be removed by more than 96% within 2 days and maintained at a low level, while the nitrate nitrogen in the control group without fillers is in a state of continuous increase.

[0079] The above-mentioned results verify that the carrier for water purification provided by the present invention can quickly start nitrification and denitrification reactions in freshwater fish tanks, save application time due to early biofilm formation, and has the performance of continuous denitrification, thus making a significant contribution to solving freshwater aquaculture water quality problems.

[0080] Example 2

[0081] The marine fish tank is filled with seawater, the stocking density is 2.5kg / 100L, and the water temperature is 25-28℃.

[0082] Step 1: Put filler A (the filler includes: 60g diatomaceous earth, 60g vermiculite powder, 40g bentonite, 40g activated clay, 30g wollastonite, 40g potassium feldspar powder, 30g dolomite powder, 30g zeolite powder, 20g manganese sand and gravel particles, 60g quartz sand particles, coagulant: foamed magnesia cement 50g) into the first 2 boxes of the 4-box filter of the aquarium fish tank, and install an aeration device at the bottom of the filter.

[0083] Step 2: Place filler B (50g of calcium-magnesium-based diatomaceous earth, 50g of expanded vermiculite powder, 100g of calcium carbonate powder, and 100g of elemental sulfur powder) into the 2 boxes after the 4-box filter of the aquarium fish tank.

[0084] Step 3: Connect the configured aquarium fish tank filter to the water in the saltwater fish tank for circulating filtration results.

[0085] At this time, for filler A, in terms of mass fraction, (diatomaceous earth + vermiculite powder) / bentonite = 3;

[0086] (Activated clay + wollastonite) / bentonite = 1.75;

[0087] (potassium feldspar powder + dolomite powder + zeolite powder + manganese sand and stone particles + quartz sand particles) / bentonite = 4.5;

[0088] Total weight of foamed magnesia cement / particle mixture = 10.8%.

[0089] For filler B, by mass, (calcium magnesium-based diatomaceous earth+expanded vermiculite powder+calcium carbonate powder) / elemental sulfur powder=2.

[0090] The mass ratio of the aerobic filler A to the anoxic filler B is 1.53.

[0091]

[0092] like Figure 2As shown in Table 2, in the experimental group using fillers in Example 2, ammonia nitrogen can be removed by 94% within 1 week and maintained at a low level; nitrite nitrogen can be completely removed within 3 days and has been stable near 0, the effect is far better than the blank group without fillers. Nitrate nitrogen can be removed by more than 96% within 3 days and maintained at a low level, while the nitrate nitrogen in the control group without fillers is in a state of continuous increase.

[0093] The above results verify that the carrier for water purification provided by the present invention can quickly start nitrification and denitrification reactions in a marine fish tank, saves application time due to early biofilm formation, and has the performance of continuous denitrification, thus making a significant contribution to solving the problem of marine aquaculture water quality.

[0094] Example 3

[0095] A South American shrimp farm in Rizhao, Shandong, cement culture pond, pond capacity 100m 3 , salinity 1.5%-2%, breeding density: 0.5kg / 100L, water temperature 20-25℃. Make a round plant aeration barrel and an autotrophic denitrification filter barrel, and connect the bottoms of the above two barrels through pipes.

[0096] The first step: put filler A (the filler includes: 120kg diatomaceous earth, 120kg vermiculite powder, 80kg bentonite, 80kg activated clay, 60kg wollastonite, 80kg potassium feldspar powder, 60kg dolomite powder, 60kg zeolite powder, 40kg manganese sand and gravel particles, 120kg quartz sand particles, coagulant: 100kg foamed magnesia cement) into an aeration barrel, fill it with seawater, and aerate it with a fan.

[0097] Step 2: Place filler B (100kg of calcium-magnesium-based diatomaceous earth, 100kg of expanded vermiculite powder, 200kg of calcium carbonate powder, and 200kg of elemental sulfur powder) into the denitrification filter barrel and fill it with seawater.

[0098] Step 3: Connect the configured filtration system to the breeding pond for circulating filtration.

[0099] At this time, for filler A, in terms of mass fraction, (diatomaceous earth + vermiculite powder) / bentonite = 3;

[0100] (Activated clay + wollastonite) / bentonite = 1.75;

[0101] (potassium feldspar powder + dolomite powder + zeolite powder + manganese sand and stone particles + quartz sand particles) / bentonite = 4.5;

[0102] Total weight of foamed magnesia cement / particle mixture = 10.8%.

[0103] For filler B, by mass, (calcium magnesium-based diatomaceous earth+expanded vermiculite powder+calcium carbonate powder) / elemental sulfur powder=2.

[0104] The mass ratio of the aerobic filler A to the anoxic filler B is 1.53.

[0105]

[0106]

[0107] like Figure 3 As shown in Table 3, in the experimental group using fillers in Example 3, 90% of ammonia nitrogen can be removed within 1 week and maintained at a low level; nitrite nitrogen can be completely removed within 3 days and has been stable near 0, the effect is far better than the blank group without fillers, 96% of nitrate nitrogen can be removed within 3 days and maintained at a low level, while the nitrate nitrogen in the control group without fillers is in a state of continuous increase.

[0108] The above results verify that the carrier for water purification provided by the present invention can quickly start nitrification and denitrification reactions in South American shrimp farms. The early biofilm formation saves application time and has the performance of continuous denitrification, which has a significant effect in solving the aquaculture water quality problem in South American shrimp farms.

[0109] Example 4

[0110] A grouper factory farm in Enshi, Hubei Province, cement breeding pond, with a capacity of 150m 3 , salinity 0.2%-0.6%, breeding density: 1.5kg / 100L, water temperature 20-25℃. Make a round plant aeration barrel and an autotrophic denitrification filter barrel, and connect the bottoms of the above two barrels through pipes.

[0111] The first step: put filler A (the filler includes: 120kg diatomaceous earth, 120kg vermiculite powder, 80kg bentonite, 80kg activated clay, 60kg wollastonite, 80kg potassium feldspar powder, 60kg dolomite powder, 60kg zeolite powder, 40kg manganese sand and gravel particles, 120kg quartz sand particles, coagulant: 100kg foamed magnesia cement) into an aeration barrel, fill it with fresh water, and use a fan for aeration.

[0112] Step 2: Place filler B (100kg of calcium-magnesium-based diatomaceous earth, 100kg of expanded vermiculite powder, 200kg of calcium carbonate powder, and 200kg of elemental sulfur powder) into the denitrification filter barrel and fill it with seawater.

[0113] Step 3: Connect the configured filtration system to the breeding pond for circulating filtration.

[0114] At this time, for filler A, in terms of mass fraction, (diatomaceous earth + vermiculite powder) / bentonite = 3;

[0115] (Activated clay + wollastonite) / bentonite = 1.75;

[0116] (potassium feldspar powder + dolomite powder + zeolite powder + manganese sand and stone particles + quartz sand particles) / bentonite = 4.5;

[0117] Total weight of foamed magnesia cement / particle mixture = 10.8%.

[0118] For filler B, by mass, (calcium magnesium-based diatomaceous earth+expanded vermiculite powder+calcium carbonate powder) / elemental sulfur powder=2.

[0119] The mass ratio of the aerobic filler A to the anoxic filler B is 1.53.

[0120]

[0121]

[0122] like Figure 4 As shown in Table 4, in the experimental group using fillers in Example 4, ammonia nitrogen can be removed by 95% within 1 week and maintained at a low level; nitrite nitrogen can be completely removed within 4 days and has been stable near 0, the effect is far better than the blank group without fillers, nitrate nitrogen can be removed by more than 98% within 6 days and maintained at a low level, while the nitrate nitrogen in the control group without fillers is in a state of continuous increase.

[0123] The above-mentioned results verify that the carrier for water purification provided by the present invention can quickly start nitrification and denitrification reactions in freshwater grouper farms. It saves application time due to early biofilm formation and has the performance of continuous denitrification, and has a significant effect in solving the aquaculture water quality problem in freshwater grouper farms.

[0124] Example 5

[0125] A koi breeding farm in Zhoukou, Henan, cement breeding pond, with a capacity of 150m 3 , salinity 0.05%-0.11%, breeding density: 2kg / 100L, water temperature 20-25℃. Make a round plant aeration barrel and an autotrophic denitrification filter barrel, and connect the bottoms of the above two barrels through pipes.

[0126] The first step: put filler A (the filler includes: 120kg diatomaceous earth, 120kg vermiculite powder, 80kg bentonite, 80kg activated clay, 60kg wollastonite, 80kg potassium feldspar powder, 60kg dolomite powder, 60kg zeolite powder, 40kg manganese sand and gravel particles, 120kg quartz sand particles, coagulant: 100kg foamed magnesia cement) into an aeration barrel, fill it with fresh water, and use a fan for aeration.

[0127] Step 2: Place filler B (100kg of calcium-magnesium-based diatomaceous earth, 100kg of expanded vermiculite powder, 200kg of calcium carbonate powder, and 200kg of elemental sulfur powder) into the denitrification filter barrel and fill it with seawater.

[0128] Step 3: Connect the configured filtration system to the breeding pond for circulating filtration.

[0129] At this time, for filler A, in terms of mass fraction, (diatomaceous earth + vermiculite powder) / bentonite = 3;

[0130] (Activated clay + wollastonite) / bentonite = 1.75;

[0131] (potassium feldspar powder + dolomite powder + zeolite powder + manganese sand and stone particles + quartz sand particles) / bentonite = 4.5;

[0132] Total weight of foamed magnesia cement / particle mixture = 10.8%.

[0133] For filler B, by mass, (calcium magnesium-based diatomaceous earth+expanded vermiculite powder+calcium carbonate powder) / elemental sulfur powder=2.

[0134] The mass ratio of the aerobic filler A to the anoxic filler B is 1.53.

[0135]

[0136] like Figure 5 As shown in Table 5, in the experimental group using fillers in Example 5, ammonia nitrogen can be removed by 95% within 1 week and maintained at a low level; nitrite nitrogen can be completely removed within 6 days and has been stable at around 0, the effect is far better than the blank group without fillers, nitrate nitrogen can be removed by more than 99% within 7 days and maintained at a low level, while the control group without fillers is inferior to the filler group.

[0137] The above-mentioned results verify that the carrier for water purification provided by the present invention can quickly start nitrification and denitrification reactions in koi farms, saves application time due to early biofilm formation, and has the performance of continuous denitrification, which has a significant effect in solving the water quality problem in koi farms.

[0138] The above examples are sufficient to illustrate that the new filler provided in this solution can simultaneously remove ammonia nitrogen, nitrate nitrogen and nitrate nitrogen from water bodies, and can provide a suitable habitat for autotrophic nitrifying bacteria and sulfur autotrophic denitrifying bacteria through the filler's own characteristics, and can effectively remove ammonia nitrogen, nitrate nitrogen and nitrite nitrogen from water without the need for an additional carbon source. Under the above-mentioned proportional relationship, countless combinations of components can also be designed to verify the problem, which will not be described in detail in this solution.

[0139] It should be noted that the above-mentioned embodiments all involve fillers A and fillers B, and their preparation methods all include a biofilm formation process, where biofilm formation is a bacterial culture process, and successful biofilm formation means successful preparation of the filler; wherein the bacteria attached to filler A are autotrophic nitrifying bacteria, and the bacteria attached to filler B are autotrophic denitrifying bacteria. In the following embodiments, relevant experiments are carried out with filler A (autotrophic nitrifying bacteria have been attached) or filler B (autotrophic denitrifying bacteria have been attached), and the relevant bacteria are no longer described separately.

[0140] Technical features not described in the present invention can be achieved through or by adopting existing technologies and will not be described in detail here. Of course, the above description is not a limitation of the present invention, and the present invention is not limited to the above examples. Changes, modifications, additions or substitutions made by ordinary technicians in this technical field within the essential scope of the present invention should also fall within the scope of protection of the present invention.

Claims

1. A novel filler for purifying aquaculture water, characterized in that: It comprises aerobic filler A, anoxic filler B, autotrophic nitrifying bacteria, autotrophic denitrifying bacteria, and water, wherein the aerobic filler A comprises a particle mixture and volcanic rock particles, and the particle mixture comprises diatomaceous earth, vermiculite powder, bentonite, activated clay, wollastonite, potassium feldspar powder, dolomite powder, zeolite powder, manganese sand and gravel particles, quartz sand particles, and a coagulant; The oxygen-deficient filler B includes calcium-magnesium-based diatomaceous earth, expanded vermiculite powder, calcium carbonate powder, and elemental sulfur powder; The mass ratio of the aerobic filler A to the anoxic filler B is (1-3): (1.5-3).

2. A novel filler for aquaculture water purification according to claim 1, characterized in that: The content of each component in the particle mixture is as follows by mass: 30-60g diatomaceous earth, 30-60g vermiculite powder, 20-40g bentonite, 20-40g activated clay, 10-30g wollastonite, 20-40g potassium feldspar powder, 10-30g dolomite powder, 10-30g zeolite powder, 10-20g manganese sand and gravel particles, and 50-60g quartz sand particles. The coagulant is 20-50g foamed magnesite cement.

3. A novel filler for aquaculture water purification according to claim 2, characterized in that: In terms of mass, the mass ratio of the particle mixture to the volcanic rock particles is 1:0.5-3, and the content of autotrophic nitrifying bacteria is 1000ppm / 1kg aerobic filler A, so that the content of sodium nitrate and sodium nitrite is between 80-200ppm; the particle size of the volcanic rock particles is 1.5-3cm.

4. A novel filler for purifying aquaculture water according to claim 2, characterized in that: In terms of mass fraction, (diatomaceous earth + vermiculite powder) / bentonite = 0.5-3; (Activated clay + wollastonite) / bentonite = 0.5-3; (potassium feldspar powder + dolomite powder + zeolite powder + manganese sand and stone particles + quartz sand particles) / bentonite = 0.5-10; Total weight of foamed magnesia cement / particle mixture = 10%-20%.

5. The novel filler for aquaculture water purification according to claim 1, characterized in that: The contents of the various components in the anoxic filler B are as follows by mass: 40-50 g of calcium-magnesium-based diatomaceous earth, 40-50 g of expanded vermiculite powder, 50-100 g of calcium carbonate powder, and 50-100 g of elemental sulfur powder.

6. A novel filler for aquaculture water purification according to claim 5, characterized in that: The content of the autotrophic denitrifying bacteria is 1000 ppm / 1 kg of aerobic filler B by mass, so that the content of sodium nitrate and sodium nitrite is between 80-200 ppm.

7. A novel filler for aquaculture water purification according to claim 6, characterized in that: By mass, (calcium magnesium based diatomaceous earth + expanded vermiculite powder + calcium carbonate powder) / elemental sulfur powder = 1-5.

8. A preparation process of a novel filler for purifying aquaculture water, using the novel filler for purifying aquaculture water as claimed in any one of claims 1 to 7, characterized in that: The specific steps include: Step S1: Mix the various components in the particle mixture, add 10-30 g of water, and press into spherical particles with a particle size of 1.5-3 cm; Step S2: mixing the spherical particles in step S1 with volcanic rock particles at a mass ratio of 1:0.5-3 to form aerobic filler A; Step S3: immersing the aerobic filler A in step S2 into water, adding about 1000 ppm of autotrophic nitrifying bacteria per 1 kg of filler, maintaining the content of sodium nitrate and sodium nitrite between 80-200 ppm, until a biofilm is formed on the surface of the filler; Step S4: storing the material obtained in step S3 in a cold storage at 0-4°C to obtain a finished product; Step S5: uniformly mix the various components in the oxygen-deficient filler B, and heat them in a sandwich pot to between 115-120° C. until the elemental sulfur in the raw material is fully dissolved and uniformly mixed with other ingredients; Step S6: granulating the material in step S5 into particles with a particle size of 1-5 cm, and cooling them for later use; Step S7: immersing the granular filler obtained in step S6 in water, adding about 1000 ppm of autotrophic denitrifying bacteria per 1 kg of filler, maintaining the content of sodium nitrate and sodium nitrite between 80-200 ppm, until a biofilm is formed on the surface of the filler; Step S8: storing the material in step S7 in a cold storage at 0-4°C to obtain a finished product; Step S9: Finally, the finished product obtained in step S4 is fully mixed with the finished product obtained in step S8 in a mass ratio of (1-3): (1.5-3) to obtain a new filler.

9. The preparation process of a novel filler for aquaculture water purification according to claim 8, characterized in that: In the step S3, after adding the autotrophic nitrifying bacteria, the mixed solution is subjected to electric pulse treatment, the electric pulse intensity is 5-10V / cm, and the pulse width is 20 microseconds to 40 microseconds.

10. The preparation process of a novel filler for aquaculture water purification according to claim 8, characterized in that: In the step S7, after adding the autotrophic denitrifying bacteria, the mixed solution is subjected to electric pulse treatment, the electric pulse intensity is 8-12V / cm, and the pulse width is 15 microseconds to 25 microseconds.

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