Intelligent cell pipetting workstation

Abstract

The invention relates to the technical field of pipetting work stations, in particular to an intelligent cell pipetting work station which comprises a three-axis moving mechanism and a culture dish containing mechanism, the three-axis moving mechanism is arranged at the inner top of a sterile box, and the movable end of the three-axis moving mechanism is connected with a pipetting mechanism; the culture dish containing mechanism comprises a U-shaped containing frame, a plurality of carrying plates are arranged in the containing frame in the vertical direction at intervals, containing plates are movably arranged on the upper surfaces of the carrying plates, and culture dish positioning assemblies are arranged on the containing plates and used for containing culture dishes; the stem cell culture dishes are arranged at intervals in the vertical direction, and the push-out assembly can control the corresponding culture dishes to horizontally extend outwards, so that the culture dish accommodating mechanism not only is beneficial to saving the volume of a cell pipetting workstation, but also does not hinder cell pipetting operation.

Description

Technical Field

[0001] The invention relates to the technical field of liquid transfer workstations, in particular to an intelligent cell liquid transfer workstation. Background Art

[0002] The cell pipetting workstation is a precision instrument that can automatically complete cell and liquid transfer operations. It can accurately control the pipetting volume and operation process, reduce human errors, and improve the accuracy and repeatability of the experiment. It is mainly used in cell culture, cell experiments, drug development, gene editing and other fields.

[0003] At present, the existing cell pipetting workstation includes a sterile box, a pipetting unit, a three-axis moving mechanism and an air source. Among them, the three-axis moving mechanism is arranged inside the sterile box, and is used to control the pipetting unit to move arbitrarily inside the sterile box, and the bottom of the pipetting unit has a number of suction nozzles, which are used to connect with the pipette; the output end of the air source is connected to the pipetting unit, and is used to control the pipette to generate positive or negative pressure, thereby completing the absorption and release of the liquid. In addition, the interior of the sterile box also has a number of centrifuge tubes, dilution tubes, culture bottles, culture dishes and other containers, among which the culture dishes are used for cell culture work.

[0004] The culture dish is usually a round box-like structure with a long diameter and a narrow thickness. Therefore, when several culture dishes are placed horizontally and spaced apart inside a sterile box, a large storage area will be occupied, which will increase the volume of the cell pipetting workstation, which is not only not conducive to storage, but also not conducive to the transfer and transportation of the cell pipetting workstation. To this end, we proposed an intelligent cell pipetting workstation to solve the above drawbacks. Summary of the invention

[0005] The object of the present invention is to provide an intelligent cell pipetting workstation to solve the problems raised in the above background technology.

[0006] The present invention is realized by the following technical scheme: an intelligent cell liquid transfer workstation, comprising a sterile box and a liquid transfer mechanism, and further comprising:

[0007] A three-axis moving mechanism is arranged on the inner top of the sterile box, and the movable end of the three-axis moving mechanism is connected to the pipetting mechanism;

[0008] The culture dish accommodating mechanism comprises a U-shaped accommodating frame, wherein a plurality of loading plates are arranged at intervals in the vertical direction inside the accommodating frame, a accommodating plate is movably arranged on the upper surface of the loading plate, a culture dish positioning assembly is arranged on the accommodating plate, and the culture dish positioning assembly is used to place the culture dish;

[0009] A push-out assembly is arranged on the receiving frame, and a plurality of the push-out assemblies correspond to the loading plates one by one, and the push-out assemblies are used to control the horizontal sliding of the receiving plates on the corresponding loading plates;

[0010] Wherein, the pipetting mechanism includes a pipetting part, and the bottom of the pipetting part has a plurality of suction nozzles, which are used to connect with the pipette; when the pipetting part pushes the ejection assembly downward vertically, the corresponding accommodating plate can extend horizontally toward the outside of the accommodating frame.

[0011] Optionally, a receiving groove is provided on the top surface of the loading plate, and sliding grooves are symmetrically provided on the inner side walls of the receiving groove. The bottom of the receiving plate is located in the receiving groove, and sliding rails are symmetrically provided on the inner side walls of the receiving plate, and the sliding rails are slidably matched with the sliding grooves.

[0012] Optionally, a strip slide is penetrated through the bottom wall of the loading plate, and the strip slide is distributed along the length direction of the accommodating groove. A protruding block is provided at the bottom of the accommodating plate, and the protruding block passes through the strip slide. A return spring is also provided inside the strip slide, and the two ends of the return spring are respectively fixedly connected to one end inside the strip slide and the protruding block; in a natural state, the return spring is in a compressed state, and the protruding block is located at one end inside the strip slide.

[0013] Optionally, an adjusting part is fixedly provided at one end of the inner side of the accommodating frame, and the ejection assembly includes a moving block and a traction rope, the moving block is vertically slidably connected to the surface of the adjusting part, one end of the traction rope is connected to the moving block, and the adjusting part is also provided with threading holes corresponding to the traction ropes one by one, one end of the threading hole is located on the outer surface of the adjusting part, and the other end of the threading hole is located on the side wall of the adjusting part and below the corresponding loading plate, and the end of the traction rope away from the moving block passes through the threading hole and is connected to the protruding block.

[0014] Optionally, a winding post is provided on the lower surface of the loading plate and at the front end of the strip-shaped sliding opening, and the traction rope is wound around the corresponding winding post; when the moving block moves downward, the corresponding receiving plate extends toward the outside of the receiving frame.

[0015] Optionally, a pushing portion is fixedly provided on the outer surface of the pipetting portion, the pushing portion is in an L-shaped structure, and the width of the pushing portion is consistent with the width of the moving block.

[0016] Optionally, a mounting notch is provided on the top surface of the accommodating plate and on one side close to the adjusting portion, the culture dish positioning assembly is located in the mounting notch, the culture dish positioning assembly comprises a hinge seat, a placement seat and a rotating shaft, the hinge seat is fixedly arranged on the inner bottom surface of the mounting notch, the placement seat is a circular box-shaped structure, and the bottom of the placement seat is rotatably connected to the hinge seat through the rotating shaft;

[0017] A shaft sleeve is provided at one end of the inner part of the installation slot, one end of the rotating shaft extends into the shaft sleeve, and a torsion spring is connected between the shaft sleeve and the rotating shaft. In a natural state, the top surface of the placement seat is in a horizontal posture.

[0018] Optionally, a hidden notch is provided on the bottom surface of the accommodating plate, the top of the protruding block extends into the hidden notch, a positioning spring is provided at one end of the protruding block facing away from the return spring, and the protruding block is elastically connected to the inner wall of the hidden notch through the positioning spring;

[0019] The external fixed sleeve of the rotating shaft is provided with a driven gear, and the inner bottom surface of the mounting slot is provided with a driven rod, and the driven rod and the inner bottom surface of the mounting slot are slidably matched along their own axial direction, one end of the driven rod is movable and penetrates into the hidden slot, and the top surface of the driven rod is also provided with teeth, and the teeth are meshed with the driven gear.

[0020] Optionally, a blocking block is provided inside the slide groove and near the adjustment portion. When the slide rail abuts against the blocking block, the protruding block is not in contact with the driven rod; when the protruding block is in contact with the end of the positioning spring inside the hidden slot that is away from the positioning spring, the angle between the upper surface of the placement seat and the horizontal plane is 30°.

[0021] Optionally, the hinge seat and the rotating shaft are located at the bottom of the placement seat and close to one side of the hidden notch. When the pushing part pushes the moving block downward to the corresponding placement seat to tilt, the pipette at the bottom of the pipetting part is located directly above the hinge seat.

[0022] Compared with the prior art, the present invention provides an intelligent cell pipetting workstation having the following features:

[0023] Beneficial effects:

[0024] 1. The cell culture dishes in the present invention are arranged at intervals in the vertical direction, and have a push-out assembly capable of controlling the corresponding culture dishes to extend horizontally outward. Therefore, the culture dish accommodating mechanism in the present invention is not only conducive to saving the volume of the cell pipetting workstation, but also does not hinder the cell pipetting operation;

[0025] 2. The push-out assembly in the present invention includes a moving block and a traction rope. When the pipetting part moves downward, the moving block can be pressed by the pushing part, so that the receiving plate and the culture dish are extended outward. Therefore, the push-out assembly in the present invention does not need to be provided with an additional power source, and the corresponding culture dish is automatically extended by linkage with the pipetting part. The structure is simple and easy to use.

[0026] 3. The placement seat in the present invention is hingedly arranged in the installation slot. When the accommodating plate is extended outward to the maximum displacement, the placement seat will also rotate accordingly to maintain an inclined posture, so that the bottom of the pipette can be inserted into the lower part of the culture dish, thereby facilitating the absorption and release of liquid. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a schematic diagram of the structure of the present invention;

[0028] Figure 2 It is a schematic diagram of the three-axis moving mechanism of the present invention;

[0029] Figure 3 It is a schematic diagram of the liquid transfer mechanism of the present invention;

[0030] Figure 4 It is a schematic diagram of the culture dish containing mechanism of the present invention;

[0031] Figure 5 This is a schematic diagram of the structure of the receiving frame of the present invention;

[0032] Figure 6 This is a schematic diagram of the structure of the accommodation plate of the present invention;

[0033] Figure 7 This is a schematic diagram of the first state of the culture dish containing mechanism of the present invention;

[0034] Figure 8 This is a schematic diagram of the second state of the culture dish containing mechanism of the present invention;

[0035] Fig. 9 This is a schematic diagram of the third state of the culture dish containing mechanism of the present invention;

[0036] Fig.10 It is a schematic diagram of the structure of the carrier plate of the present invention;

[0037] Fig.11 for Figure 6 The corresponding figure at point A is enlarged.

[0038] In the figure: 100, sterile box; 200, pipetting mechanism; 201, pipetting part; 202, suction nozzle; 203, pushing part; 300, three-axis moving mechanism; 400, culture dish containing mechanism; 401, containing rack; 402, loading plate; 403, containing plate; 404, containing groove; 405, slide groove; 406, slide rail; 407, strip slide; 408, protruding block; 409, reset spring; 410, adjusting part; 411, winding column; 412, mounting notch; 413, hinge seat; 414, placement seat; 415, rotating shaft; 416, shaft sleeve; 417, blocking block; 418, hidden notch; 419, positioning spring; 420, driven gear; 421, driven rod; 422, teeth; 500, ejection assembly; 501, moving block; 502, traction rope. DETAILED DESCRIPTION

[0039] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] Example 1: Please refer to Figure 1 - Fig.11 This embodiment proposes an intelligent cell pipetting workstation, including a sterile box 100 and a pipetting mechanism 200, and also includes a three-axis moving mechanism 300, a culture dish accommodating mechanism 400 and an ejection assembly 500; wherein, the three-axis moving mechanism 300 is arranged at the inner top of the sterile box 100, and the movable end of the three-axis moving mechanism 300 is connected to the pipetting mechanism 200, which is used to control the pipetting mechanism 200 to move in the horizontal direction or vertical direction inside the sterile box 100, thereby completing various pipetting operations.

[0041] It is worth mentioning that the interior of the sterile box 100 is also provided with a number of centrifuge tubes, culture bottles, microplates, pipette tip boxes, etc., for containing various solutions or tools required for culturing cells.

[0042] The culture dish containing mechanism 400 includes a containing frame 401 in the shape of a U, and a plurality of carrier plates 402 are arranged at intervals in the vertical direction inside the containing frame 401. The carrier plates 402 are in a horizontal posture, and a containing plate 403 is movably provided on the upper surface of the carrier plates 402. A culture dish positioning assembly is provided on the containing plate 403, and the culture dish positioning assembly is used to place the culture dish; that is, a carrier plate 402 is slidably connected to a containing plate 403, and a culture dish is arranged on a containing plate 403.

[0043] Furthermore, the ejection assembly 500 is arranged on the receiving frame 401, and a plurality of ejection assemblies 500 correspond to the carrier plate 402 one by one, and the ejection assembly 500 is used to control the horizontal sliding of the receiving plate 403 on the corresponding carrier plate 402; in addition, the liquid transfer mechanism 200 includes a liquid transfer part 201, and the bottom of the liquid transfer part 201 has a plurality of suction nozzles 202, and the suction nozzles 202 are used to connect with the pipette; when the liquid transfer part 201 pushes the ejection assembly 500 to move downward vertically, the corresponding receiving plate 403 can extend horizontally toward the outside of the receiving frame 401. It should be noted that the top of the suction nozzle 202 extends into the liquid transfer part 201, and the inside of the suction nozzle 202 also has a piston, and the top of the liquid transfer part 201 is provided with a motor, which can drive the lifting and lowering of a plurality of pistons, thereby forming a negative pressure or a positive pressure inside the suction nozzle 202, so as to use the pipette to absorb or release the solution, so as to complete the transfer of the solution.

[0044] In order to achieve sliding cooperation between the loading plate 402 and the receiving plate 403, a receiving groove 404 is opened on the top surface of the loading plate 402, and sliding grooves 405 are symmetrically opened on the inner side walls of the receiving groove 404. The bottom of the receiving plate 403 is located in the receiving groove 404, and sliding rails 406 are symmetrically opened on the two side walls of the receiving plate 403. The sliding rails 406 are slidingly cooperated with the sliding grooves 405; when the receiving plate 403 slides along the sliding grooves 405, one end of the receiving plate 403 can extend to the outside of the receiving frame 401.

[0045] Furthermore, a strip-shaped sliding opening 407 is provided through the bottom wall of the loading plate 402, and the strip-shaped sliding opening 407 is distributed along the length direction of the receiving groove 404. A protruding block 408 is provided at the bottom of the receiving plate 403, and the protruding block 408 passes through the strip-shaped sliding opening 407. A return spring 409 is also provided inside the strip-shaped sliding opening 407, and the two ends of the return spring 409 are respectively fixedly connected to one end inside the strip-shaped sliding opening 407 and the protruding block 408; in a natural state, the return spring 409 is in a compressed state, and the protruding block 408 is located at one end inside the strip-shaped sliding opening 407. That is, the return spring 409 has a pushing effect on the protruding block 408, so that it is always located at one end inside the strip-shaped sliding opening 407 without being acted upon by external forces.

[0046] The following describes how the ejection assembly 500 controls the sliding movement of the receiving plate 403:

[0047] An adjusting portion 410 is fixedly provided at one end of the inner side of the accommodating frame 401, and the ejection assembly 500 includes a moving block 501 and a traction rope 502. The moving block 501 is vertically slidably connected to the surface of the adjusting portion 410, and one end of the traction rope 502 is connected to the moving block 501. The adjusting portion 410 is also provided with threading holes corresponding to the traction ropes 502. One end of the threading holes is located on the outer surface of the adjusting portion 410, and the other end of the threading holes is located on the side wall of the adjusting portion 410 and below the corresponding loading plate 402. One end of the traction rope 502 away from the moving block 501 passes through the threading hole and is connected to the protruding block 408.

[0048] In addition, a winding post 411 is provided on the lower surface of the loading plate 402 and at the front end of the strip-shaped sliding opening 407, and the traction rope 502 is wound around the corresponding winding post 411; when the moving block 501 moves downward, the corresponding receiving plate 403 extends toward the outside of the receiving frame 401, and when the moving block 501 rises, the traction rope 502 is released, and then the receiving plate 403 is gradually reset under the action of the reset spring 409. It should be noted that the projections of the plurality of moving blocks 501 on the horizontal plane are staggered, that is, the movement of a single moving block 501 does not drive the movement of other moving blocks 501.

[0049] In addition, a pushing portion 203 is fixedly provided on the outer surface of the pipetting portion 201. The pushing portion 203 is in an L-shaped structure. The width of the pushing portion 203 is consistent with the width of the moving block 501. The bottom of the pushing portion 203 is used to abut against the top surface of the moving block 501, thereby pushing the moving block 501 to move downward to push out the containing plate 403 and the culture dish.

[0050] In summary, in the specific application process of this embodiment, when it is necessary to transfer the external solution to the corresponding culture dish, or to transfer the solution in the corresponding culture dish to the external container, the three-axis moving mechanism 300 should be used to control the movement of the liquid transfer mechanism 200, and the corresponding suction nozzle 202 at the bottom of the liquid transfer part 201 should be connected to the pipette. Then the liquid transfer part 201 is driven to the position of the receiving frame 401, and the bottom of the push part 203 is aligned with the corresponding moving block 501, and then the liquid transfer part 201 is controlled to descend, so that the push part 203 pushes the moving block 501 downward, thereby pulling the traction rope 502 to pull the receiving plate 403 outward, until the bottom end of the pipette is extended into the culture dish, and finally the corresponding liquid transfer operation is completed.

[0051] After the transfer is completed, the transfer part 201 slowly rises again, and the receiving plate 403 will gradually return to its original position under the action of the return spring 409. In this way, a plurality of receiving plates 403 are stacked in the same vertical direction, thereby saving the storage space of the culture dish in the horizontal direction, so as to realize the miniaturization and lightness of the cell transfer workstation.

[0052] Example 2: Please refer to Figure 1 - Fig.11 This embodiment also proposes an intelligent cell pipetting workstation. The difference between this embodiment and the first embodiment is that:

[0053] A mounting slot 412 is provided on the top surface of the accommodating plate 403 and on one side close to the adjusting portion 410. The culture dish positioning assembly is located in the mounting slot 412. The culture dish positioning assembly includes a hinge seat 413, a placement seat 414 and a rotating shaft 415. The hinge seat 413 is fixedly arranged on the inner bottom surface of the mounting slot 412. The placement seat 414 is a circular box-shaped structure. The bottom of the placement seat 414 is rotatably connected to the hinge seat 413 through the rotating shaft 415. The interior of the placement seat 414 is used to place culture dishes of corresponding specifications. Its function is to prevent the culture dishes from moving back and forth inside the placement seat 414 and to keep the position of the culture dishes stable.

[0054] Furthermore, a sleeve 416 is provided at one end of the inner portion of the mounting slot 412, one end of the rotating shaft 415 extends into the sleeve 416, and a torsion spring is connected between the sleeve 416 and the rotating shaft 415, and in a natural state, the top surface of the placement seat 414 is in a horizontal posture. That is, the torsion spring has the function of maintaining the posture of the placement seat 414, and when the placement seat 414 is not subjected to external forces of the system, its top surface always maintains a horizontal posture.

[0055] A hidden slot 418 is provided on the bottom surface of the accommodating plate 403, and the top of the protruding block 408 extends into the hidden slot 418. A positioning spring 419 is provided at one end of the protruding block 408 facing away from the return spring 409. The protruding block 408 is elastically connected to the inner wall of the hidden slot 418 through the positioning spring 419, that is, the protruding block 408 is elastically connected in the hidden slot 418, and it should be noted that the stiffness coefficient of the positioning spring 419 is much larger than the stiffness coefficient of the return spring 409; the external fixed sleeve of the rotating shaft 415 is provided with a driven gear 420, and the inner bottom surface of the mounting slot 412 is provided with a driven rod 421, and the driven rod 421 and the inner bottom surface of the mounting slot 412 are slidably matched along their own axial direction, one end of the driven rod 421 is movable and penetrates into the hidden slot 418, and the top surface of the driven rod 421 is also provided with teeth 422, and the teeth 422 are meshed with the driven gear 420. That is, when the driven rod 421 slides along its own axial direction, it can drive the driven gear 420 to rotate, thereby driving the corresponding placement seat 414 and the culture dish to tilt.

[0056] Specifically, a blocking block 417 is provided inside the slide groove 405 and near the adjusting portion 410. When the receiving plate 403 extends horizontally outward until the slide rail 406 is in contact with the blocking block 417, Figure 8 In the state shown, the protruding block 408 is not in contact with the driven rod 421, that is, the displacement of the protruding block 408 has not yet reached the initial spacing between the protruding block 408 and the driven rod 421; when the protruding block 408 is in contact with the end of the hidden notch 418 facing away from the positioning spring 419, the angle between the upper surface of the placement seat 414 and the horizontal plane is 30°. That is, when the protruding block 408 moves to the maximum displacement, the angle between the placement seat 414 and the top surface of the culture dish and the horizontal plane is 30°, as shown in FIG. Fig. 9 In the state shown, the liquid in the culture dish will gather at a low point, making it convenient for the pipette to absorb the liquid in the culture dish.

[0057] It should be added that when the traction rope 502 pulls the protrusion block 408 to move, since the stiffness coefficient of the positioning spring 419 is much larger than the stiffness coefficient of the reset spring 409, the protrusion block 408 can pull the receiving plate 403 outward and continuously compress the reset spring 409; until the slide rail 406 abuts against the blocking block 417 inside the slide groove 405, at this time, the culture dish has completely extended out of the outside of the receiving frame 401, and the position of the receiving plate 403 no longer moves; as the traction rope 502 continues to be pulled, the protrusion block 408 can move inside the hidden slot 418, thereby pushing the driven rod 421, causing the placement seat 414 to tilt.

[0058] In addition, it should be noted that the hinge seat 413 and the rotating shaft 415 are located at the bottom of the placement seat 414 and close to the side of the hidden notch 418. When the pushing part 203 pushes the moving block 501 downward to the corresponding placement seat 414 to tilt, the pipette at the bottom of the pipetting part 201 is located just above the hinge seat 413. That is, when the protruding block 408 moves to the maximum displacement, the culture dish is in a tilted posture, and the bottom end of the pipette is just located on the inner side of the lower part of the culture dish.

[0059] In summary, in the specific application process of this embodiment, when it is necessary to transfer the external solution to the corresponding culture dish, or to transfer the solution in the corresponding culture dish to the external container, it is also necessary to use the push part 203 to press the moving block 501 downward. When the protruding block 408 moves to the maximum displacement, the culture dish is in an inclined posture, and the bottom end of the pipette is located at the inner side of the lower part of the culture dish. Specifically, when releasing the liquid, the liquid can drip on the lower part of the culture dish. Compared with the culture dish being placed flat and releasing the liquid, this method of releasing the liquid can effectively prevent the liquid from scouring the cell layer on the bottom wall of the culture dish and breaking the cells; specifically, when sucking the liquid, this method can make the liquid gather at the lower part, so that the bottom of the pipette is completely immersed in the liquid, which is convenient for sucking the liquid in the culture dish.

[0060] In addition, it is worth mentioning that since the hinge seat 413 is located at the bottom of the placement seat 414 and close to one side of the hidden slot 418, compared with the case where the hinge seat 413 is located in the middle of the bottom surface of the placement seat 414, the advantage of this embodiment is that there is no need to make the depth of the installation slot 412 large enough, which helps to reduce the distance between two adjacent carrier plates 402, that is, it helps to save the height space of the culture dish accommodating mechanism 400.

[0061] On the other hand, when the receiving plate 403 extends outward until the slide rail 406 just contacts the blocking block 417, the pipette is located at the position directly above the hinge seat 413, and then the pipette part 201 further descends, driving the placement seat 414 to rotate around the hinge seat 413, so that the culture dish maintains an inclined posture; because the position of the hinge seat 413 remains stationary, that is, the pipette descends while the lower part of the culture dish does not descend, so the bottom of the pipette can be immersed in the solution in the culture dish. If the hinge seat 413 is located in the middle of the bottom of the placement seat 414, then when the placement seat 414 rotates, the lower part of the culture dish will also move downward, making it impossible for the bottom of the pipette to be inserted into the lower part of the culture dish.

[0062] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0063] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An intelligent cell pipetting workstation, comprising a sterile box (100) and a pipetting mechanism (200), characterized in that: It further includes: A three-axis moving mechanism (300), which is arranged on the inner top of the sterile box body (100), and the movable end of the three-axis moving mechanism (300) is connected to the pipetting mechanism (200); A petri dish accommodating mechanism (400), which includes a U-shaped accommodating frame (401). Inside the accommodating frame (401), a plurality of carrier plates (402) are arranged at intervals in the vertical direction. An accommodating plate (403) is movably arranged on the upper surface of the carrier plate (402). A petri dish positioning assembly is arranged on the accommodating plate (403), and the petri dish positioning assembly is used for placing petri dishes; A pushing-out assembly (500), which is arranged on the accommodating frame (401). A plurality of the pushing-out assemblies (500) correspond to the carrier plates (402) one by one, and the pushing-out assembly (500) is used for controlling the horizontal sliding of the accommodating plate (403) on the corresponding carrier plate (402); Among them, the pipetting mechanism (200) includes a pipetting part (201). The bottom of the pipetting part (201) has a plurality of suction nozzles (202), and the suction nozzles (202) are used for connecting with pipettes; when the pipetting part (201) pushes the pushing-out assembly (500) downward along the vertical direction, the corresponding accommodating plate (403) can horizontally extend toward the outside of the accommodating frame (401).

2. An intelligent cell pipetting workstation according to claim 1, characterized in that: A receiving groove (404) is formed in the top surface of the carrier plate (402). Sliding grooves (405) are symmetrically formed in the inner side walls on both sides of the receiving groove (404). The bottom of the accommodating plate (403) is located in the receiving groove (404). Slide rails (406) are symmetrically formed on both side walls of the accommodating plate (403), and the slide rails (406) are in sliding fit with the sliding grooves (405).

3. An intelligent cell pipetting workstation according to claim 2, characterized in that: A strip-shaped sliding opening (407) is formed through the bottom wall of the carrier plate (402), and the strip-shaped sliding opening (407) is distributed along the length direction of the receiving groove (404). A protruding block (408) is arranged at the bottom of the accommodating plate (403), and the protruding block (408) passes through the strip-shaped sliding opening (407). A return spring (409) is further arranged inside the strip-shaped sliding opening (407), and both ends of the return spring (409) are fixedly connected to one end inside the strip-shaped sliding opening (407) and the protruding block (408) respectively; in the natural state, the return spring (409) is in a compressed state, and the protruding block (408) is located at one end inside the strip-shaped sliding opening (407).

4. The intelligent cell liquid transfer workstation according to claim 3, characterized in that: An adjusting portion (410) is fixedly provided at one end of the inner side of the accommodating frame (401), and the ejecting assembly (500) comprises a moving block (501) and a traction rope (502), wherein the moving block (501) is vertically slidably connected to the surface of the adjusting portion (410), and one end of the traction rope (502) is connected to the moving block (501), and threading holes corresponding to the traction ropes (502) are also provided on the adjusting portion (410), one end of the threading hole is located on the outer surface of the adjusting portion (410), and the other end of the threading hole is located on the side wall of the adjusting portion (410) and below the corresponding loading plate (402), and one end of the traction rope (502) away from the moving block (501) passes through the threading hole and is connected to the protruding block (408).

5. The intelligent cell liquid transfer workstation according to claim 4, characterized in that: A winding post (411) is provided on the lower surface of the loading plate (402) and at the front end of the strip-shaped sliding opening (407), and the traction rope (502) is wound around the corresponding winding post (411); when the moving block (501) moves downward, the corresponding receiving plate (403) extends toward the outside of the receiving frame (401).

6. The intelligent cell liquid transfer workstation according to claim 4, characterized in that: A pushing portion (203) is fixedly provided on the outer surface of the liquid transfer portion (201); the pushing portion (203) is in an L-shaped structure; and the width of the pushing portion (203) is consistent with the width of the moving block (501).

7. The intelligent cell liquid transfer workstation according to claim 6, characterized in that: A mounting notch (412) is provided on the top surface of the accommodating plate (403) and on one side close to the adjusting portion (410); the culture dish positioning assembly is located in the mounting notch (412); the culture dish positioning assembly comprises a hinge seat (413), a placement seat (414) and a rotating shaft (415); the hinge seat (413) is fixedly arranged on the inner bottom surface of the mounting notch (412); the placement seat (414) is a circular box-shaped structure; the bottom of the placement seat (414) is rotatably connected to the hinge seat (413) via the rotating shaft (415); A shaft sleeve (416) is provided at one end of the interior of the installation slot (412), one end of the rotating shaft (415) extends into the shaft sleeve (416), and a torsion spring is connected between the shaft sleeve (416) and the rotating shaft (415). In a natural state, the top surface of the placement seat (414) is in a horizontal position.

8. The intelligent cell liquid transfer workstation according to claim 7, characterized in that: A hidden notch (418) is provided on the bottom surface of the accommodating plate (403), the top of the protruding block (408) extends into the hidden notch (418), a positioning spring (419) is provided on one end of the protruding block (408) facing away from the return spring (409), and the protruding block (408) is elastically connected to the inner wall of the hidden notch (418) via the positioning spring (419); The external fixed sleeve of the rotating shaft (415) is provided with a driven gear (420), the inner bottom surface of the mounting slot (412) is provided with a driven rod (421), the driven rod (421) and the inner bottom surface of the mounting slot (412) are slidably matched along their own axial direction, one end of the driven rod (421) is movable and penetrates into the hidden slot (418), and the top surface of the driven rod (421) is also provided with teeth (422), and the teeth (422) are meshed with the driven gear (420).

9. The intelligent cell liquid transfer workstation according to claim 8, characterized in that: A blocking block (417) is provided inside the slide groove (405) and at a position close to the adjustment portion (410); when the slide rail (406) abuts against the blocking block (417), the protruding block (408) is not in contact with the driven rod (421); when the protruding block (408) is in contact with one end of the hidden notch (418) facing away from the positioning spring (419), the angle between the upper surface of the placement seat (414) and the horizontal plane is 30°.

10. The intelligent cell liquid transfer workstation according to claim 8, characterized in that: The hinge seat (413) and the rotating shaft (415) are located at the bottom of the placement seat (414) and close to one side of the hidden notch (418). When the pushing part (203) pushes the moving block (501) downward to the corresponding placement seat (414) to tilt, the pipette at the bottom of the pipetting part (201) is located directly above the hinge seat (413).

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