Cell transfection device and transfection method

Abstract

The invention belongs to the technical field of cell transfection, and particularly relates to a cell transfection device and a transfection method.The cell transfection device comprises a base used for supporting; the first driving assembly is mounted at the top of the base; the second driving assembly is mounted at the top of the first driving assembly; the bearing assembly is mounted at the top of the second driving assembly; and the dropwise adding assembly is mounted on one side wall of the base. The first driving assembly and the second driving assembly can drive the carrier plate to reciprocate in a cross track, so that the culture dish is driven to be automatically shaken up in a transfection compound preparation process, and a reagent is added under a shaking condition; therefore, the reagent can be dropwise added in different parts of the inner cavity of the culture dish in the moving process of the culture dish, the homogenization effect of the reagent can be improved, meanwhile, original manual shaking is replaced, and the situation that the reagent is actually adhered to the side wall of the culture dish due to excessive shaking operation can be avoided.

Description

Technical Field

[0001] The invention relates to the technical field of cell transfection, in particular to a cell transfection device and a transfection method. Background Art

[0002] Transfection is the process by which cells acquire new phenotypes by actively or passively introducing exogenous DNA fragments under certain conditions. In essence, it is no different from transformation. Whether it is transfection or transformation, the key factor is to treat bacteria or cultured cells with calcium chloride to increase the permeability of the cell membrane, so that exogenous DNA or RNA can easily enter the cell;

[0003] In the existing cell transfection process, the staff will first use the pipetting gun to introduce the required reagents and culture medium into the culture dish, so that the transfection complex is formed in the culture dish for transfection culture. The culture dish needs to be shaken during the transfection complex configuration process, but currently the transfection complex is shaken after the configuration is completed, so when adding reagents, they are often added at fixed points, and the reagents gather at the addition point, which is not conducive to the subsequent shaking effect. In addition, manual shaking is prone to excessive shaking, causing the reagents to adhere to the side wall of the inner cavity of the culture dish, which is further not conducive to the uniform distribution of the transfection complex at the bottom of the culture dish. For this reason, a new technical solution needs to be designed to solve the problem. Summary of the invention

[0004] The purpose of the present invention is to provide a cell transfection device and a transfection method, which solve the problems raised in the background technology.

[0005] To achieve the above-mentioned object, the present invention provides the following technical solution: A cell transfection device, characterized in that it comprises: a base for supporting;

[0006] A first driving assembly is installed on the top of the base and is used to drive the culture dish to shake;

[0007] A second driving assembly is installed on the top of the first driving assembly and is used to drive the culture dish to rotate and adjust;

[0008] A bearing assembly is installed on the top of the second driving assembly and is used to carry and position the culture dish;

[0009] The dripping assembly is installed on one side wall of the base, and is used to limit the sample adding gun and push the sample adding gun to automatically drip the reagent during the shaking process.

[0010] By adopting the above technical scheme, the culture dish is placed on the supporting component for positioning, and then the sample adding gun is limited by the dropping component. Then, when configuring the transfection complex, after the culture medium is configured, the first driving component is used to drive the supporting component and the culture dish to move back and forth along the length direction of the base to achieve a shaking operation, and the dropping component is used to drive the sample adding gun to drop the reagent, so as to achieve shaking and dropping at the same time. The reagent can be dropped at different parts of the inner cavity of the culture dish during the movement of the culture dish, which is beneficial to improving the homogenization effect of the reagent, and at the same time replaces the previous manual shaking, which is beneficial to avoid the actual adhesion to the side wall of the culture dish caused by excessive shaking. At the same time, the transfection complex is configured while shaking, which can improve the efficiency of transfection.

[0011] As a preferred embodiment of the present invention, the first driving component comprises:

[0012] A fixed plate is fixedly mounted on the top of the base, and a sliding groove is provided on the top of the fixed plate;

[0013] A screw motor is fixed to one end face of the inner cavity of the slide groove. The outer end of the transmission shaft of the screw motor is fixedly connected to a screw. The outer end of the screw is rotatably connected to the other end of the inner cavity of the slide groove. The outer wall of the screw is covered with an adaptive screw slider, and the screw slider is connected to the second drive component.

[0014] As a preferred embodiment of the present invention, the second driving assembly comprises:

[0015] A carrier block is fixed on the top of the screw slider;

[0016] A servo motor, a mounting groove is provided on the top of the carrier block, the servo motor is embedded in the mounting groove, and the outer end of the transmission shaft of the servo motor is connected to the bearing assembly.

[0017] As a preferred embodiment of the present invention, a third driving assembly is further provided on the base, and the third driving assembly includes:

[0018] The gear is arranged in a strip groove opened on the top of the base. The gear is fixed to the outer wall of the short shaft, and the short shaft rotates through the base and is fixedly connected to the dripping assembly.

[0019] During the reciprocating movement of the second driving component, the culture dish will move along with its moving direction, and then the liquid in the culture dish will also move along with the moving direction. At this time, the second driving component drives the spur rack to move. Under the condition that the spur rack driving gear rotates, it can drive the side plate to rotate, thereby driving the entire dripping component to rotate, so that the dosing gun is tilted, and the angle of the liquid outlet of the dosing gun can be changed, so that the liquid outlet of the dosing gun can cater to the direction of liquid flow in the culture dish, so that the added reagent can collide with the flowing liquid, and then under the collision condition, the added reagent can be quickly diffused, which is beneficial to further enhance the shaking effect after addition. Similarly, when moving in the reverse direction, it can drive the dosing gun to tilt in the reverse direction.

[0020] As a preferred embodiment of the present invention, the dripping assembly comprises:

[0021] A side panel, arranged on one side of the base;

[0022] A limit block is arranged on one side of the side plate and is fixedly connected to the side plate through a connecting rod, and a limit hole is provided on the top of the limit block for limiting the tail end of the sample adding gun;

[0023] The electric push rod is arranged just above the limit block. The electric push rod is fixed to the top of the horizontal plate, and one end of the horizontal plate is fixedly connected to the side plate. The push rod tail end of the electric push rod is fixedly connected to the push block.

[0024] As a preferred embodiment of the present invention, a slide rail is fixed on one side of the top of the base, and matching sliders are slidably installed on both sides of the top of the slide rail. The top of the slider is fixedly connected to the side wall of the spur rack through a connecting block.

[0025] As a preferred embodiment of the present invention, a groove is formed at the bottom of the push block.

[0026] As a preferred embodiment of the present invention, rubber blocks are embedded at the four corners of the bottom of the base.

[0027] The present invention also relates to a cell transfection method, comprising the following steps:

[0028] Step 1: first introduce the culture medium into the culture dish, and place the culture dish on the carrying component for carrying positioning, and place the sample adding gun in the dripping component for positioning;

[0029] Step 2: Use the first driving component to drive the culture dish to move back and forth, so as to achieve the shaking operation of the culture dish. At the same time, the dripping component will drive the sample adding gun to drip the reagent. When dripping the reagent, when the first driving component drives the second driving component and the culture dish to move to one side, the dripping component can be tilted under the driving of the third driving component, so that the liquid outlet of the sample adding gun caters to the moving direction of the liquid in the culture dish to add the reagent;

[0030] Step 3: After the first driving component drives the culture dish to move back and forth and shake a certain number of times, the second driving component drives the culture dish to rotate ninety degrees, and then the first driving component is continued to drive the culture dish to shake, and the reagent is added by the dropping component during shaking. After the shaking is completed, the culture dish is placed in the incubator for culture.

[0031] Compared with the prior art, the present invention has the following beneficial effects:

[0032] The present invention can drive the carrier plate to move back and forth in a cross track through the first driving component and the second driving component, so as to achieve automatic shaking of the culture dish during the process of configuring the transfection complex, so that the reagent can be added under the condition of shaking, so that the reagent can be dripped at different parts of the inner cavity of the culture dish during the movement of the culture dish, which is beneficial to improving the homogenization effect of the reagent, and replaces the previous manual shaking, which is beneficial to avoid the actual adhesion of the culture dish side wall caused by excessive shaking operation, and the shaking operation while configuring the transfection complex can improve the transfection efficiency;

[0033] The side plate can be driven to rotate by the third driving component, so that when the second driving component moves, the dripping component can be driven to rotate, so as to change the angle of the liquid outlet of the sample adding gun, so that the liquid outlet of the sample adding gun can cater to the direction of liquid flow in the culture dish, so that the added reagent can collide with the flowing liquid, and then under the collision condition, the added reagent can be quickly diffused, which is conducive to further improving the shaking effect after addition. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Other features, objects and advantages of the present invention will become more apparent from the detailed description of non-limiting embodiments made with reference to the following drawings:

[0035] Figure 1 It is a schematic diagram of the overall structure of a cell transfection device and a transfection method of the present invention;

[0036] Figure 2 It is a schematic structural diagram of a cell transfection device and a transfection method of the present invention after disassembling the bearing components;

[0037] Figure 3It is a schematic diagram of the structure of a carrier component of a cell transfection device and a transfection method of the present invention;

[0038] Figure 4 The figure is a rear structural schematic diagram of a cell transfection device and a transfection method of the present invention.

[0039] In the figure:

[0040] 1. Base; 11. Strip groove; 12. Slide rail; 13. Slider;

[0041] 2. Side plate; 21. Horizontal plate; 22. Electric push rod; 23. Push block; 24. Limit block;

[0042] 3. Fixed plate; 31. Screw motor; 32. Screw; 33. Screw slider; 34. Carrying block; 35. Servo motor;

[0043] 4. Connecting plate; 41. Spur rack; 42. Gear. DETAILED DESCRIPTION

[0044] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0045] In the description of the present invention, it is necessary to understand that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside" and "outside" etc., indicating directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation on the present invention.

[0046] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected" and "set" should be understood in a broad sense, for example, they can be fixedly connected or set, or detachably connected or set, or integrally connected or set. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances. The models of electrical appliances provided in the present invention are only for reference, and different models of electrical appliances with the same functions can be replaced according to actual usage.

[0047] See also Figure 1-4 , the present invention provides a technical solution: a cell transfection device, comprising a base 1 for support;

[0048] A first driving assembly is installed on the top of the base 1 and is used to drive the culture dish to shake;

[0049] A second driving assembly is installed on the top of the first driving assembly and is used to drive the culture dish to rotate and adjust;

[0050] A bearing assembly is installed on the top of the second driving assembly and is used to carry and position the culture dish;

[0051] The dripping assembly is installed on a side wall of the base 1, and is used to limit the sample adding gun and push the sample adding gun to automatically drip the reagent during the shaking process.

[0052] It should be understood that when the transfection complex is actually configured, the culture dish is placed on the supporting component for positioning, and then the sample adding gun is limited by the dripping component. Then, when the transfection complex is configured, after the culture medium is configured, the first driving component is used to drive the supporting component and the culture dish to move back and forth along the length direction of the base 1 to achieve a shaking operation, and the dripping component is used to drive the sample adding gun to drip the reagent, thereby achieving shaking and dripping at the same time. The reagent can be dripped at different parts of the inner cavity of the culture dish during the movement of the culture dish, which is beneficial to improving the homogenization effect of the reagent, and at the same time replaces the previous manual shaking, which is beneficial to avoid the actual adhesion to the side wall of the culture dish caused by excessive shaking. At the same time, the transfection complex is configured while shaking, which can improve the efficiency of transfection.

[0053] Furthermore, rubber blocks are embedded at the four corners of the bottom of the base 1. The provision of the rubber blocks helps prevent the entire device from sliding when it is placed on a desktop for use.

[0054] Specifically, the first driving component includes:

[0055] The fixing plate 3 is fixedly mounted on the top of the base 1, and a sliding groove is provided on the top of the fixing plate 3;

[0056] The screw motor 31 is fixed to one end face of the inner cavity of the chute, the outer end of the transmission shaft of the screw motor 31 is fixedly connected with a screw 32, the outer end of the screw 32 is rotatably connected to the other end of the inner cavity of the chute, the outer wall of the screw 32 is sleeved with an adapted screw slider 33, and the screw slider 33 is connected to the second drive assembly;

[0057] It should be understood that the screw motor 31 can drive the screw 32 to rotate, thereby driving the screw slider 33 to move, so that the screw motor 31 can drive the second drive component, the supporting component and the culture dish to move back and forth through forward and reverse rotation, thereby driving the culture dish to shake evenly.

[0058] It should be noted that the second driving component includes:

[0059] The carrier block 34 is fixed on the top of the screw slider 33;

[0060] Servo motor 35, a mounting groove is provided on the top of the carrier block 34, the servo motor 35 is embedded in the mounting groove, and the outer end of the transmission shaft of the servo motor 35 is connected to the bearing assembly;

[0061] It should be understood that after the first driving component drives the culture dish to shake back and forth a certain number of times in the length direction of the base 1, the servo motor 35 is used to drive the supporting component to rotate ninety degrees, that is, the culture dish is rotated ninety degrees, and then the first driving component is used again to drive the culture dish to shake back and forth, which is equivalent to driving the culture dish to achieve a cross-track shaking.

[0062] like Figure 1 and 2 As shown; the dripping assembly includes:

[0063] A side panel 2 is provided on one side of the base 1;

[0064] The limit block 24 is arranged on one side of the side plate 2 and is fixedly connected to the side plate 2 through a connecting rod. A limit hole is provided on the top of the limit block 24 for limiting the tail end of the sample adding gun;

[0065] The electric push rod 22 is arranged just above the limit block 24. The electric push rod 22 is fixed to the top of the horizontal plate 21 and one end of the horizontal plate 21 is fixedly connected to the side plate 2. The push rod tail end of the electric push rod 22 is fixedly connected to the push block 23.

[0066] It should be understood that the tail end of the sample adding gun is inserted into the limiting hole at the top of the limiting block 24, and then the electric push rod 22 is used to drive the push block 23 to fit the tail end of the sample adding gun, so as to limit the sample adding gun. Then, when adding reagents, the electric push rod 22 is used to drive the push block 23 to push the sample adding gun to realize the sample adding operation.

[0067] Furthermore, a groove is provided at the bottom of the push block 23, and the groove can be sleeved on the tail end of the sample adding gun to ensure the stability of the limit.

[0068] As a further preferred embodiment, a third driving assembly is further provided on the base 1, and the third driving assembly includes:

[0069] The gear 42 is disposed in the strip groove 11 opened at the top of the base 1. The gear 42 is fixed to the outer wall of the short shaft, and the short shaft rotates through the base 1 and is fixedly connected to the drip assembly, specifically connected to the side plate 2. The short shaft and the gear 42 can drive the side plate 2 to rotate;

[0070] A spur rack 41 is disposed above the gear 42 and meshes with the gear 42. The spur rack 41 is fixedly connected to the side wall of the second driving assembly through the connecting plate 4. The spur rack 41 is used to drive the gear 42 to rotate;

[0071] It should be understood that the connecting plate 4 is specifically fixedly connected to the side wall of the carrier block 34. During the reciprocating movement of the second driving component, the culture dish will move along its moving direction, and then the liquid in the culture dish will also move along the moving direction. At this time, the second driving component drives the spur rack 41 to move. Under the condition that the spur rack 41 drives the gear 42 to rotate, it can drive the side plate 2 to rotate, thereby driving the entire dripping component to rotate, so that the sample adding gun is tilted, and the angle of the liquid outlet of the sample adding gun can be changed, so that the liquid outlet of the sample adding gun can cater to the direction of liquid flow in the culture dish, so that the added reagent can collide with the flowing liquid, and then under the collision condition, the added reagent can be quickly diffused, which is beneficial to further enhance the shaking effect after addition. Similarly, when moving in the reverse direction, it can drive the sample adding gun to tilt in the reverse direction.

[0072] Furthermore, a slide rail 12 is fixed to one side of the top of the base 1, and matching sliders 13 are slidably mounted on both sides of the top of the slide rail 12, and the top of the slider 13 is fixedly connected to the side wall of the spur rack 41 through a connecting block;

[0073] Therefore, the sliding block 13 is used to slide on the slide rail 12 in a limited track, which can help improve the stability of the movement of the spur rack 41.

[0074] A cell transfection method is specifically implemented as follows:

[0075] Step 1: first introduce the culture medium into the culture dish, and place the culture dish on the carrying component for carrying positioning, and place the sample adding gun in the dripping component for positioning;

[0076] Step 2: Use the first driving component to drive the culture dish to move back and forth, so as to achieve the shaking operation of the culture dish. At the same time, the dripping component will drive the sample adding gun to drip the reagent. When dripping the reagent, when the first driving component drives the second driving component and the culture dish to move to one side, the dripping component can be tilted under the driving of the third driving component, so that the liquid outlet of the sample adding gun caters to the moving direction of the liquid in the culture dish to add the reagent;

[0077] Step 3: After the first driving component drives the culture dish to move back and forth and shake a certain number of times, the second driving component drives the culture dish to rotate ninety degrees, and then the first driving component is continued to drive the culture dish to shake, and the reagent is added by the dropping component during shaking. After the shaking is completed, the culture dish is placed in the incubator for culture.

[0078] In addition, the components included in a cell transfection device and transfection method of the present invention are all universal standard parts or components known to technical personnel in this field, and their structures and principles can be known to technical personnel through technical manuals or through conventional experimental methods. In the idle part of the device, all the above-mentioned electrical components, which refer to power elements, electrical components, and adapted monitoring computers and power supplies, are connected through wires. The specific connection means should refer to the following working principle. The electrical connection is completed in the working order of each electrical component. The detailed connection means are well-known technologies in this field. The following mainly introduces the working principle and process, and no longer explains the electrical control.

[0079] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the attached claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention. Any figure mark in the claims should not be regarded as limiting the claims involved.

[0080] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A cell transfection device, characterized in that: include: A base (1) for support; A first driving assembly is mounted on the top of the base (1) and is used to drive the culture dish to shake; A second driving assembly is installed on the top of the first driving assembly and is used to drive the culture dish to rotate and adjust; A bearing assembly is installed on the top of the second driving assembly and is used to carry and position the culture dish; The dripping assembly is mounted on a side wall of the base (1) and is used to limit the position of the sample adding gun and to push the sample adding gun to automatically drip the reagent during the shaking process.

2. A cell transfection device according to claim 1, characterized in that: The first driving assembly comprises: A fixed plate (3) is fixedly mounted on the top of the base (1), and a sliding groove is provided on the top of the fixed plate (3); A screw motor (31) is fixed to one end surface of the inner cavity of the slide groove; the outer end of the transmission shaft of the screw motor (31) is fixedly connected to a screw (32); the outer end of the screw (32) is rotatably connected to the other end of the inner cavity of the slide groove; an outer wall of the screw (32) is sleeved with an adapted screw slider (33); and the screw slider (33) is connected to a second drive assembly.

3. A cell transfection device according to claim 2, characterized in that: The second driving assembly comprises: A carrier block (34) is fixed on the top of the screw slider (33); A servo motor (35), a mounting groove is provided on the top of the carrier block (34), the servo motor (35) is embedded in the mounting groove, and the outer end of the transmission shaft of the servo motor (35) is connected to the bearing assembly.

4. A cell transfection device according to claim 1, characterized in that: The base (1) is also provided with a third driving assembly, the third driving assembly comprising: The gear (42) is arranged in a strip groove (11) opened on the top of the base (1). The gear (42) is fixed to the outer wall of the short shaft, and the short shaft rotates through the base (1) and is fixedly connected to the dripping assembly.

5. A cell transfection device according to claim 1, characterized in that: The dripping component comprises: A side plate (2) is arranged on one side of the base (1); A limit block (24) is arranged on one side of the side plate (2) and is fixedly connected to the side plate (2) via a connecting rod. A limit hole is provided on the top of the limit block (24) for limiting the rear end of the sample adding gun; The electric push rod (22) is arranged just above the limit block (24). The electric push rod (22) is fixed to the top of the horizontal plate (21) and one end of the horizontal plate (21) is fixedly connected to the side plate (2). The push rod tail end of the electric push rod (22) is fixedly connected to the push block (23).

6. A cell transfection device according to claim 4, characterized in that: A slide rail (12) is fixed to one side of the top of the base (1), and matching sliders (13) are slidably mounted on both sides of the top of the slide rail (12), and the top of the slider (13) is fixedly connected to the side wall of the spur rack (41) via a connecting block.

7. A cell transfection device according to claim 5, characterized in that: The bottom of the push block (23) is provided with a groove.

8. A cell transfection device according to claim 1, characterized in that: Rubber blocks are embedded at the four corners of the bottom of the base (1).

9. A cell transfection method according to claim 1, applicable to the cell transfection device according to claims 1-8, characterized in that: The method comprises the following steps: Step 1: first introduce the culture medium into the culture dish, and place the culture dish on the carrying component for carrying positioning, and place the sample adding gun in the dripping component for positioning; Step 2: Use the first driving component to drive the culture dish to move back and forth, so as to achieve the shaking operation of the culture dish. At the same time, the dripping component will drive the sample adding gun to drip the reagent. When dripping the reagent, when the first driving component drives the second driving component and the culture dish to move to one side, the dripping component can be tilted under the driving of the third driving component, so that the liquid outlet of the sample adding gun caters to the moving direction of the liquid in the culture dish to add the reagent; Step 3: After the first driving component drives the culture dish to move back and forth and shake a certain number of times, the second driving component drives the culture dish to rotate ninety degrees, and then the first driving component is continued to drive the culture dish to shake, and the reagent is added by the dropping component during shaking. After the shaking is completed, the culture dish is placed in the incubator for culture.

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