Biocatalytic conversion device based on polypeptide

Abstract

The invention relates to the technical field of catalytic reaction devices, and discloses a polypeptide-based biological catalytic conversion device which comprises a catalytic reaction kettle, a protective top cover is fixedly mounted at the top of the catalytic reaction kettle, an electric heating seat is arranged outside the catalytic reaction kettle, an electric control discharge valve is fixedly connected to the bottom of the catalytic reaction kettle, and a controller is fixedly mounted on the electric heating seat. A stirring main shaft is arranged in the middle of the protective top cover, a plurality of stirring blade mechanisms are arranged below the exterior of the stirring main shaft, and a spiral shaft is inserted into the stirring main shaft. According to the biological catalytic conversion device based on polypeptide, a catalyst is put into the multiple stirring blade mechanisms along the stirring main shaft, when the stirring main shaft drives the multiple stirring blade mechanisms to rotate, the catalyst can make contact with a substrate through the through holes, catalytic conversion is conducted on the substrate, meanwhile, the stirring blade bodies are arranged to be S-shaped, the contact area of the catalyst and the substrate is increased, and the catalytic conversion efficiency is improved. And the catalyst can be prevented from being sheared and damaged by the stirring shaft while ensuring that the catalyst can be in full contact with a substrate.

Description

Technical Field

[0001] The invention relates to the technical field of catalytic reaction devices, in particular to a biocatalytic conversion device based on polypeptides. Background Art

[0002] Peptide biocatalytic conversion is the process of converting substrates into peptides or modifying peptides using biocatalysts (such as enzymes). The peptide biocatalytic conversion process includes substrate selection, catalyst selection, reaction condition optimization, catalytic conversion reaction, product separation and purification, product identification, application, process optimization and large-scale production.

[0003] The biocatalytic conversion of polypeptides is mostly carried out in a reactor, which can achieve an efficient, specific and environmentally friendly biocatalytic process under low temperature and low pressure conditions. The reactor used for the biocatalytic conversion of polypeptides is usually a stirred tank type. The stirred tank reactor is mainly composed of three parts: a kettle body, an agitator and a heat preservation device. It has the advantages of simple structure, sufficient and uniform mixing of catalyst and substrate, easy control of temperature and pH, and the ability to handle colloidal substrates and insoluble substrates. However, when catalytically converting polypeptides, the catalyst is directly put into the reactor, and the catalyst needs to be separated by filtration, centrifugation, chromatography and other operations. The catalyst recovery is difficult, resulting in a significant increase in process costs; in addition, the catalyst particles are easily destroyed by the shear force generated when the agitator rotates, which increases the difficulty of catalyst recovery and the recovery rate is low. In addition, the broken catalyst will also affect the purity of the product. Summary of the invention

[0004] The object of the present invention is to provide a polypeptide-based biocatalytic conversion device to solve the problems raised in the above background technology.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a polypeptide-based biocatalytic conversion device, comprising a catalytic reactor, wherein a protective top cover is fixedly installed on the top of the catalytic reactor, an electric heating seat is arranged on the outside, and an electric-controlled discharge valve is fixedly connected to the bottom, a controller is fixedly installed on the electric heating seat, a stirring main shaft is arranged in the middle of the protective top cover, and a plurality of stirring blade mechanisms are arranged below the outside of the stirring main shaft, a spiral shaft is inserted inside the stirring main shaft, and a discharge pipe is arranged above the outside of the stirring main shaft, a driving motor is fixedly installed on the top of the protective top cover, which is used to provide power for the stirring main shaft and the spiral shaft to rotate, a transmission assembly is arranged on the output shaft of the driving motor, a lower ratchet mechanism is arranged on the outside of the top end of the stirring main shaft, which is used to limit the stirring main shaft to rotate in one direction, an upper ratchet mechanism is arranged on the outside of the top end of the spiral shaft, which is used to limit the spiral shaft to rotate in another direction, the upper ratchet mechanism and the lower ratchet mechanism are arranged in a mirror image, and a transmission cage is arranged between the upper ratchet mechanism and the lower ratchet mechanism;

[0006] The stirring blade mechanism comprises a stirring blade body, a through hole and a material return slope. The open end of the stirring blade body is fixedly connected to the stirring main shaft, and the interior of the stirring blade body is filled with catalyst particles.

[0007] Preferably, a bearing seat cooperating with the stirring shaft is provided in the middle part of the top of the protective top cover, and the stirring shaft is rotatably connected to the middle part of the protective top cover through the bearing seat, a feeding port is provided in the front of the top of the protective top cover, and a positioning cantilever is fixedly installed at the rear of the top, the top of the screw shaft is movably connected to the positioning cantilever, and the screw shaft can be auxiliary restricted by the positioning cantilever to ensure the stability of the screw shaft during rotation.

[0008] Preferably, a through groove matching the size of the stirring blade mechanism is provided at the connection between the stirring main shaft and the stirring blade mechanism, the stirring blade mechanism is shaped like an "S" when viewed from above, the surface of the stirring blade body is provided with evenly distributed through holes, and the diameter of the through holes is slightly smaller than the diameter of the catalyst particles, and a return material slope is provided at the bottom of the stirring blade body. When the spiral shaft rotates in the stirring main shaft, the catalyst in the stirring blade body can slide along the return material slope into the stirring main shaft, and the catalyst can be discharged along the discharge pipe when the spiral shaft rotates.

[0009] Preferably, the upper ratchet mechanism and the lower ratchet mechanism have the same structure, both comprising an inner ring seat, an outer ring seat, a limit ratchet, a mounting groove, a limit pawl and a top spring. The outer ring seat is movably mounted on the inner ring seat, and the inner wall of the outer ring seat is provided with a plurality of evenly distributed limit ratchets. The inner ring seat is provided with a plurality of mounting grooves, and the plurality of mounting grooves are movably installed with limit pawls and top springs. The upper ratchet mechanism and the lower ratchet mechanism are used for restriction, and the stirring main shaft or the spiral shaft can be independently driven to rotate by controlling the forward and reverse rotation of the output shaft of the driving motor, thereby realizing stirring of the substrate during the catalytic reaction and convenient recovery of the catalyst.

[0010] Preferably, the top spring always applies elastic force to the limit pawl. After the inner ring seat and the outer ring seat are assembled, the limit pawl can be clamped on the limit ratchet on the inner wall of the outer ring seat under the elastic force of the top spring. The top spring applies elastic force to the limit pawl to ensure that the limit pawl can stably cooperate with the limit ratchet on the inner wall of the outer ring seat, thereby realizing stable transmission between the outer ring seat and the inner ring seat.

[0011] Preferably, the transmission assembly includes an active synchronous gear, a driven synchronous gear and a synchronous belt. The active synchronous gear is fixedly mounted on the output shaft of the drive motor, the driven synchronous gear is fixedly mounted on the outer ring seat in the lower ratchet mechanism, and the synchronous belt is meshingly mounted on the outside of the active synchronous gear and the driven synchronous gear. The active synchronous gear, the driven synchronous gear and the synchronous belt are driven to ensure that the drive motor can drive the stirring main shaft or the spiral shaft to rotate, thereby achieving stirring of the substrate or recovery of the catalyst particles.

[0012] Preferably, the inner ring seat in the lower ratchet mechanism is fixedly mounted on the top of the stirring main shaft, the inner ring seat in the upper ratchet mechanism is fixedly mounted on the top of the spiral shaft, the top of the transmission cage is fixedly mounted on the outside of the outer ring seat in the upper ratchet mechanism, and the bottom end is fixedly connected to the top of the driven synchronous gear, and the transmission is transmitted through the transmission cage, and the driven synchronous gear is connected to the outer ring seat in the upper ratchet mechanism, ensuring that the driving motor can drive the spiral shaft to rotate through the transmission assembly and the upper ratchet mechanism.

[0013] Preferably, the angle between the center axis of the discharge pipe and the center axis of the stirring main shaft is 75-85°, and the discharge pipe is located between the lower ratchet mechanism and the bearing seat, which limits the position of the discharge pipe and ensures that the discharge pipe can discharge the catalyst particles inside the stirring main shaft.

[0014] Preferably, the spiral shaft consists of a central shaft and spiral blades, and the spiral blades of the spiral shaft are tangent to the inner wall of the stirring main shaft. The top of the spiral blades of the spiral shaft is located 1-3 cm above the discharge pipe, ensuring that the spiral shaft can stably transport the catalyst particles to the discharge pipe to achieve the recovery of the catalyst particles.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] 1. The polypeptide-based biocatalytic conversion device, by putting the catalyst into a plurality of stirring blade mechanisms along the stirring main shaft, when the stirring main shaft drives the plurality of stirring blade mechanisms to rotate, the catalyst can contact with the substrate through the through hole to catalytically convert the substrate, and at the same time, the stirring blade body is set to be S-shaped to increase the contact area between the catalyst and the substrate, ensuring that the catalyst can fully contact with the substrate while avoiding the catalyst being sheared and damaged by the stirring shaft, thereby avoiding affecting the catalyst recovery rate and product purity.

[0017] 2. The polypeptide-based biocatalytic conversion device drives the motor output shaft to rotate counterclockwise and transmits through the transmission component and the lower ratchet mechanism, which can drive the stirring main shaft and several stirring blade mechanisms to rotate, so as to stir the substrate in the catalytic reactor. The motor output shaft drives the motor to rotate clockwise and transmits through the transmission component, the transmission cage and the upper ratchet mechanism, which can drive the spiral shaft to rotate in the stirring main shaft. The catalyst in the stirring blade body can slide along the return material inclined plane in the stirring main shaft. When the spiral shaft rotates, the catalyst can be discharged along the discharge pipe, so as to realize the convenient recovery of the catalyst.

[0018] 3. The polypeptide-based biocatalytic conversion device is restricted by an upper ratchet mechanism and a lower ratchet mechanism. By controlling the forward and reverse rotation of the output shaft of the drive motor, the stirring main shaft or the spiral shaft can be independently driven to rotate, thereby achieving stirring of the substrate during the catalytic reaction and convenient recovery of the catalyst, improving the degree of integration, and reducing the number of drive motors and reducing costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the structure after the overall combination of the present invention;

[0020] Figure 2 It is a structural schematic diagram of the cross section of the catalytic reactor and the protective top cover in the present invention;

[0021] Figure 3 It is a structural schematic diagram of the cross section of the stirring main shaft in the present invention;

[0022] Figure 4 It is a structural schematic diagram of the stirring blade mechanism in the present invention;

[0023] Figure 5 It is a structural schematic diagram of the transmission assembly in the present invention;

[0024] Figure 6 It is a schematic diagram of the structure of the top of the protective cover in the present invention;

[0025] Figure 7 It is a schematic structural diagram of the connection between the upper ratchet mechanism and the lower ratchet mechanism in the present invention;

[0026] Figure 8 It is a schematic diagram of the structure of the inner ring seat and the outer ring seat in the present invention;

[0027] Fig. 9 It is a structural schematic diagram of the explosion of the lower ratchet mechanism in the present invention.

[0028] In the figure: 1. Catalytic reactor; 2. Protective top cover; 3. Bearing seat; 4. Stirring main shaft; 5. Stirring blade mechanism; 51. Stirring blade body; 52. Through hole; 53. Return material slope; 6. Discharge pipe; 7. Screw shaft; 8. Upper ratchet mechanism; 9. Lower ratchet mechanism; 91. Inner ring seat; 92. Outer ring seat; 93. Limit ratchet; 94. Mounting groove; 95. Limit pawl; 96. Top spring; 10. Transmission cage; 11. Transmission assembly; 111. Active synchronous gear; 112. Driven synchronous gear; 113. Synchronous belt; 12. Driving motor; 13. Positioning cantilever; 14. Electric heating seat; 15. Controller; 16. Electric control discharge valve. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] See also Figures 1 to 9In an embodiment of the present invention, a polypeptide-based biocatalytic conversion device includes a catalytic reactor 1, a protective top cover 2 is fixedly installed on the top of the catalytic reactor 1, an electric heating seat 14 is arranged on the outside, and an electric-controlled discharge valve 16 is fixedly connected to the bottom, a controller 15 is fixedly installed on the electric heating seat 14, the controller 15 is electrically connected to the electric heating seat 14 and the drive motor 12, and the controller 15, the electric heating seat 14, and the drive motor 12 are all prior art and are commercially available, a stirring main shaft 4 is arranged in the middle of the protective top cover 2, and a plurality of stirring blade mechanisms 5 are arranged below the outside of the stirring main shaft 4, a spiral shaft 7 is inserted inside the stirring main shaft 4, and a discharge pipe 6 is arranged above the outside of the stirring main shaft 4, a drive motor 12 is fixedly installed on the top of the protective top cover 2 for providing power for the rotation of the stirring main shaft 4 and the spiral shaft 7, a transmission assembly 11 is arranged on the output shaft of the drive motor 12, a lower ratchet mechanism 9 is arranged on the outer side of the top of the stirring main shaft 4 for limiting the stirring main shaft 4 to rotate in one direction, and an upper ratchet mechanism 9 is arranged on the outer side of the top of the spiral shaft 7 The wheel mechanism 8 is used to limit the spiral shaft 7 from rotating in another direction. The upper ratchet mechanism 8 and the lower ratchet mechanism 9 are used to limit the rotation. The output shaft of the driving motor 12 can independently drive the stirring main shaft 4 or the spiral shaft 7 to rotate by controlling the forward and reverse rotation of the output shaft of the driving motor 12, so as to realize the stirring of the substrate during the catalytic reaction and the convenient recovery of the catalyst, thereby improving the degree of integration. The upper ratchet mechanism 8 and the lower ratchet mechanism 9 are arranged in a mirror image, and a transmission cage 10 is arranged between the upper ratchet mechanism 8 and the lower ratchet mechanism 9. The output shaft of the driving motor 12 rotates counterclockwise and is transmitted through the transmission component 11 and the lower ratchet mechanism 9, so as to drive the stirring main shaft 4 and a plurality of stirring blade mechanisms 5 to rotate, so as to stir the substrate in the catalytic reactor 1. The output shaft of the driving motor 12 rotates clockwise and is rotated through the transmission component 11, the transmission cage 10 and the upper ratchet mechanism 8, so as to drive the spiral shaft 7 to rotate in the stirring main shaft 4. The catalyst in the stirring blade body 51 can slide along the return material inclined surface 53 into the stirring main shaft 4. When the spiral shaft 7 rotates, the catalyst can be discharged along the discharge pipe 6, so as to realize the convenient recovery of the catalyst.

[0031] The stirring blade mechanism 5 includes a stirring blade body 51, a through hole 52 and a return material slope 53. The open end of the stirring blade body 51 is fixedly connected to the stirring main shaft 4. The interior of the stirring blade body 51 is filled with catalyst particles. When the stirring main shaft 4 drives the plurality of stirring blade mechanisms 5 to rotate, the catalyst can contact the substrate through the through hole 52 to catalytically convert the substrate. At the same time, the stirring blade body 51 is set to an S shape to increase the contact area between the catalyst and the substrate, thereby ensuring that the catalyst can fully contact the substrate and preventing the catalyst from being sheared and damaged by the stirring shaft.

[0032] As a further implementation scheme of the above invention: a bearing seat 3 cooperating with the stirring spindle 4 is arranged in the middle part of the top of the protective top cover 2, and the stirring spindle 4 is rotatably connected to the middle part of the protective top cover 2 through the bearing seat 3, and the stirring spindle 4 is restricted by the bearing seat 3 to ensure that the stirring spindle 4 is firmly mounted on the protective top cover 2 and can rotate stably, a feeding port is arranged in front of the top of the protective top cover 2, and a positioning cantilever 13 is fixedly installed at the rear of the top, the top of the screw shaft 7 is movably connected to the positioning cantilever 13, and the screw shaft 7 can be auxiliary restricted by the positioning cantilever 13 to ensure the stability of the screw shaft 7 during rotation.

[0033] As a further implementation scheme of the above invention: a through groove adapted to the size of the stirring blade mechanism 5 is provided at the connection between the stirring shaft 4 and the stirring blade mechanism 5, and the stirring shaft 4 and the stirring blade mechanism 5 are connected through the through groove to ensure that the catalyst particles can flow along the through groove between the stirring shaft 4 and the stirring blade body 51, and the shape of the stirring blade mechanism 5 when viewed from above is "S", and the surface of the stirring blade body 51 is provided with evenly distributed through holes 52, and the diameter of the through holes 52 is slightly smaller than the diameter of the catalyst particles, so as to ensure that the catalyst particles can contact the substrate in the catalytic reactor 1 along the through holes 52 while avoiding the leakage of catalyst particles along the stirring blade body 51, and a return material slope 53 is provided at the bottom of the stirring blade body 51, and when the spiral shaft 7 rotates in the stirring shaft 4, the catalyst in the stirring blade body 51 can slide along the return material slope 53 into the stirring shaft 4, and when the spiral shaft 7 rotates, the catalyst can be discharged along the discharge pipe 6, thereby realizing convenient recovery of the catalyst.

[0034] As a further implementation scheme of the above invention: the upper ratchet mechanism 8 and the lower ratchet mechanism 9 have the same structure, both of which include an inner ring seat 91, an outer ring seat 92, a limiting ratchet 93, a mounting groove 94, a limiting pawl 95 and a top spring 96. The upper ratchet mechanism 8 and the lower ratchet mechanism 9 are restricted, and the stirring main shaft 4 or the spiral shaft 7 can be independently driven to rotate by controlling the forward and reverse rotation of the output shaft of the drive motor 12, so as to achieve stirring of the substrate during the catalytic reaction and convenient recovery of the catalyst, improve the degree of integration, reduce the number of drive motors, and reduce costs. The outer ring seat 92 is movably mounted on the inner ring seat 91, and the inner wall of the outer ring seat 92 is provided with a plurality of evenly distributed limiting ratchets 93, and the inner ring seat 91 is provided with a plurality of mounting grooves 9 4, and a plurality of mounting grooves 94 are movably mounted with limit pawls 95 and top springs 96, the upper ratchet mechanism 8 and the lower ratchet mechanism 9 are arranged in a mirror image, when the driven synchronous gear 112 rotates counterclockwise, the driven synchronous gear 112 drives the outer ring seat 92 in the lower ratchet mechanism 9 and drives the outer ring seat 92 in the upper ratchet mechanism 8 to rotate counterclockwise through the transmission cage 10, the top spring 96 applies elastic force to the limit pawl 95, and is restricted by the limit ratchet 93 and a plurality of limit pawls 95, at this time, the outer ring seat 92 in the lower ratchet mechanism 9 can drive the inner ring seat 91 in the lower ratchet mechanism 9 to rotate when the outer ring seat 92 in the upper ratchet mechanism 8 rotates counterclockwise, and the inner ring seat 91 in the upper ratchet mechanism 8 cannot be driven to rotate when the outer ring seat 92 in the upper ratchet mechanism 8 rotates counterclockwise.

[0035] As a further implementation scheme of the above invention: the top spring 96 always applies elastic force to the limit pawl 95. After the inner ring seat 91 and the outer ring seat 92 are assembled, the limit pawl 95 can be clamped on the limit ratchet 93 on the inner wall of the outer ring seat 92 under the elastic force of the top spring 96. The elastic force applied to the limit pawl 95 by the top spring 96 ensures that the limit pawl 95 can stably cooperate with the limit ratchet 93 on the inner wall of the outer ring seat 92, thereby realizing stable transmission between the outer ring seat 92 and the inner ring seat 91.

[0036] As a further implementation scheme of the above invention: the transmission component 11 includes an active synchronous gear 111, a driven synchronous gear 112 and a synchronous toothed belt 113. The active synchronous gear 111 is fixedly mounted on the output shaft of the drive motor 12, and the transmission is carried out through the gears and the toothed belt to ensure the transmission efficiency and avoid slipping. The driven synchronous gear 112 is fixedly mounted on the outer ring seat 92 in the lower ratchet mechanism 9, and the synchronous toothed belt 113 is meshingly mounted on the outside of the active synchronous gear 111 and the driven synchronous gear 112. The transmission is carried out through the active synchronous gear 111, the driven synchronous gear 112 and the synchronous toothed belt 113 to ensure that the drive motor 12 can drive the stirring main shaft 4 or the spiral shaft 7 to rotate, thereby realizing the stirring of the substrate or the recovery of the catalyst particles.

[0037] As a further implementation scheme of the above invention: the inner ring seat 91 in the lower ratchet mechanism 9 is fixedly mounted on the top of the stirring main shaft 4, and the lower ratchet mechanism 9 is transmission-connected to the stirring main shaft 4, so that the driving motor 12 can drive the stirring main shaft 4 to rotate, thereby realizing the stirring of the substrate; the inner ring seat 91 in the upper ratchet mechanism 8 is fixedly mounted on the top of the spiral shaft 7, and the upper ratchet mechanism 8 is transmission-connected to the stirring main shaft 4, so that the driving motor 12 can drive the spiral shaft 7 to rotate, thereby realizing the recovery of catalyst particles; the top end of the transmission cage 10 is fixedly mounted on the outside of the outer ring seat 92 in the upper ratchet mechanism 8, and the bottom end is fixedly connected to the top of the driven synchronous gear 112, and the transmission is carried out through the transmission cage 10, and the driven synchronous gear 112 is transmission-connected to the outer ring seat 92 in the upper ratchet mechanism 8, thereby ensuring that the driving motor 12 can drive the spiral shaft 7 to rotate through the transmission assembly 11 and the upper ratchet mechanism 8.

[0038] As a further implementation scheme of the above invention: the angle between the central axis of the discharge pipe 6 and the central axis of the stirring main shaft 4 is 75-85°, and the discharge pipe 6 is tilted to facilitate the discharge of catalyst particles inside the stirring main shaft 4. The discharge pipe 6 is located between the lower ratchet mechanism 9 and the bearing seat 3, and the position of the discharge pipe 6 is restricted to ensure that the discharge pipe 6 can discharge the catalyst particles inside the stirring main shaft 4, while preventing the discharge pipe 6 from affecting the rotation of the upper ratchet mechanism 8, the lower ratchet mechanism 9 and the transmission cage 10.

[0039] As a further implementation scheme of the above invention: the spiral shaft 7 is composed of a central shaft and spiral blades, and the spiral blades of the spiral shaft 7 are tangent to the inner wall of the stirring main shaft 4, ensuring the conveying effect of the catalyst particles when the spiral shaft 7 rotates, and the top of the spiral blades of the spiral shaft 7 is located 1-3 cm above the discharge pipe 6, ensuring that the spiral shaft 7 can stably convey the catalyst particles to the discharge pipe 6, thereby realizing the recovery of the catalyst particles.

[0040] During the specific implementation, the catalyst particles required for the biocatalytic conversion of the polypeptide are put into the stirring main shaft 4 along the top of the stirring main shaft 4, and the stirring main shaft 4 is connected to a plurality of stirring blade bodies 51. After entering the stirring main shaft 4, the catalyst particles can flow into the plurality of stirring blade bodies 51, and the substrate required for the biocatalytic conversion of the polypeptide is put into the catalytic reactor 1 along the feeding port, and the electric heating seat 14 is controlled by the controller 15 to adjust the internal temperature of the catalytic reactor 1; the upper ratchet mechanism 8 and the lower ratchet mechanism 9 are arranged in a mirror image, and when the driven synchronous gear 112 rotates counterclockwise, the driven synchronous gear 112 drives the outer ring seat 92 in the lower ratchet mechanism 9 and drives the outer ring seat 92 in the upper ratchet mechanism 8 to rotate counterclockwise through the transmission cage 10, and the top spring 96 is used to limit The positioning pawl 95 applies elastic force and is limited by the limiting ratchet 93 and a plurality of limiting pawls 95. At this time, the outer ring seat 92 in the lower ratchet mechanism 9 rotates counterclockwise to drive the inner ring seat 91 in the lower ratchet mechanism 9 to rotate, and the outer ring seat 92 in the upper ratchet mechanism 8 rotates counterclockwise and cannot drive the inner ring seat 91 in the upper ratchet mechanism 8 to rotate; when the driven synchronous gear 112 rotates clockwise, at this time, the outer ring seat 92 in the upper ratchet mechanism 8 rotates clockwise to drive the inner ring seat 91 in the upper ratchet mechanism 8 to rotate, and the outer ring seat 92 in the lower ratchet mechanism 9 rotates clockwise and cannot drive the inner ring seat 91 in the lower ratchet mechanism 9 to rotate; the controller 15 controls the output shaft of the drive motor 12 to rotate counterclockwise, and through the active synchronous gear 111 and the synchronous belt 11 3 and the driven synchronous gear 112 drive, drive the outer ring seat 92 and the inner ring seat 91 in the lower ratchet mechanism 9 to rotate counterclockwise, thereby driving the stirring main shaft 4 and the plurality of stirring blade mechanisms 5 to rotate. At this time, the spiral shaft 7 is in a non-rotating state, stirring the substrate in the catalytic reactor 1, so that the catalyst particles in the stirring blade body 51 contact with the substrate along the through hole 52, and the stirring blade body 51 is S-shaped as a whole, which increases the contact area between the catalyst particles and the substrate, ensures that the catalyst particles can fully contact with the substrate, and avoids the catalyst particles being sheared and damaged by the stirring shaft, thereby avoiding affecting the recovery rate of the catalyst particles and the purity of the product; after the polypeptide biocatalytic conversion, the controller 15 controls the electronically controlled discharge valve 16 to open, and the catalytic reactor 1 The product is discharged; the controller 15 controls the output shaft of the drive motor 12 to rotate clockwise, and drives the outer ring seat 92 and the inner ring seat 91 in the upper ratchet mechanism 8 to rotate clockwise through the active synchronous gear 111, the synchronous toothed belt 113 and the driven synchronous gear 112, driving the spiral shaft 7 to rotate. At this time, the stirring shaft 4 is in a non-rotating state, and the catalyst particles in the stirring shaft 4 are transported upward by the rotating spiral shaft 7 and discharged along the discharge pipe 6 to achieve the recovery of the catalyst particles. The bottom of the stirring blade body 51 is an inclined surface. When the catalyst particles in the stirring shaft 4 are reduced, the catalyst particles in the stirring blade body 51 can slide along the return material inclined surface 53 into the stirring shaft 4 to achieve the recovery of the catalyst particles in the stirring blade body 51.

[0041] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

Claims

1. A polypeptide-based biocatalytic conversion device, comprising a catalytic reactor (1), wherein a protective top cover (2) is fixedly installed on the top of the catalytic reactor (1), an electric heating seat (14) is arranged on the outside, and an electric-controlled discharge valve (16) is fixedly connected to the bottom, and a controller (15) is fixedly installed on the electric heating seat (14), characterized in that: A stirring main shaft (4) is arranged in the middle of the protective top cover (2), and a plurality of stirring blade mechanisms (5) are arranged below the outside of the stirring main shaft (4). A spiral shaft (7) is inserted into the inside of the stirring main shaft (4), and a discharge pipe (6) is arranged above the outside of the stirring main shaft (4). A driving motor (12) is fixedly installed on the top of the protective top cover (2) for providing power for the stirring main shaft (4) and the spiral shaft (7) to rotate. A transmission assembly (11) is arranged on the output shaft of the driving motor (12). A lower ratchet mechanism (9) is arranged on the outside of the top end of the stirring main shaft (4) for limiting the stirring main shaft (4) to rotate in one direction. An upper ratchet mechanism (8) is arranged on the outside of the top end of the spiral shaft (7) for limiting the spiral shaft (7) to rotate in another direction. The upper ratchet mechanism (8) and the lower ratchet mechanism (9) are arranged in a mirror image, and a transmission cage (10) is arranged between the upper ratchet mechanism (8) and the lower ratchet mechanism (9); The stirring blade mechanism (5) comprises a stirring blade body (51), a through hole (52) and a material return slope (53); the open end of the stirring blade body (51) is fixedly connected to the stirring main shaft (4); and the interior of the stirring blade body (51) is filled with catalyst particles.

2. A polypeptide-based biocatalytic conversion device according to claim 1, characterized in that: A bearing seat (3) matched with the stirring main shaft (4) is arranged in the middle of the top of the protective top cover (2), and the stirring main shaft (4) is rotatably connected to the middle of the protective top cover (2) through the bearing seat (3). A feeding port is arranged in front of the top of the protective top cover (2), and a positioning cantilever (13) is fixedly installed at the rear of the top. The top of the spiral shaft (7) is movably connected to the positioning cantilever (13).

3. A polypeptide-based biocatalytic conversion device according to claim 1, characterized in that: A through groove matching the size of the stirring blade mechanism (5) is provided at the connection between the stirring main shaft (4) and the stirring blade mechanism (5); the stirring blade mechanism (5) is shaped like an "S" when viewed from above; the surface of the stirring blade body (51) is provided with evenly distributed through holes (52); and the diameter of the through holes (52) is slightly smaller than the diameter of the catalyst particles; and a return material slope (53) is provided at the bottom of the stirring blade body (51).

4. A polypeptide-based biocatalytic conversion device according to claim 1, characterized in that: The upper ratchet mechanism (8) and the lower ratchet mechanism (9) have the same structure, both comprising an inner ring seat (91), an outer ring seat (92), a limiting ratchet (93), a mounting groove (94), a limiting pawl (95) and a top spring (96). The outer ring seat (92) is movably mounted on the inner ring seat (91). The inner wall of the outer ring seat (92) is provided with a plurality of evenly distributed limiting ratchet teeth (93). The inner ring seat (91) is provided with a plurality of mounting grooves (94), and the limiting pawls (95) and the top springs (96) are movably installed in the plurality of mounting grooves (94).

5. A polypeptide-based biocatalytic conversion device according to claim 4, characterized in that: The top spring (96) always applies elastic force to the limit pawl (95). After the inner ring seat (91) and the outer ring seat (92) are assembled, the limit pawl (95) can be clamped on the limit ratchet (93) on the inner wall of the outer ring seat (92) under the elastic force of the top spring (96).

6. A polypeptide-based biocatalytic conversion device according to claim 5, characterized in that: The transmission assembly (11) comprises a driving synchronous gear (111), a driven synchronous gear (112) and a synchronous toothed belt (113); the driving synchronous gear (111) is fixedly mounted on the output shaft of the driving motor (12); the driven synchronous gear (112) is fixedly mounted on the outer ring seat (92) in the lower ratchet mechanism (9); and the synchronous toothed belt (113) is meshingly mounted on the outside of the driving synchronous gear (111) and the driven synchronous gear (112).

7. A polypeptide-based biocatalytic conversion device according to claim 6, characterized in that: The inner ring seat (91) in the lower ratchet mechanism (9) is fixedly mounted on the top of the stirring main shaft (4), the inner ring seat (91) in the upper ratchet mechanism (8) is fixedly mounted on the top of the spiral shaft (7), the top of the transmission cage (10) is fixedly mounted on the outside of the outer ring seat (92) in the upper ratchet mechanism (8), and the bottom end is fixedly connected to the top of the driven synchronous gear (112).

8. A polypeptide-based biocatalytic conversion device according to claim 2, characterized in that: The angle between the central axis of the discharge pipe (6) and the central axis of the stirring main shaft (4) is 75-85°, and the discharge pipe (6) is located between the lower ratchet mechanism (9) and the bearing seat (3).

9. A polypeptide-based biocatalytic conversion device according to claim 1, characterized in that: The spiral shaft (7) is composed of a central shaft and spiral blades, and the spiral blades of the spiral shaft (7) are tangent to the inner wall of the stirring main shaft (4), and the top of the spiral blades of the spiral shaft (7) is located 1-3 cm above the discharge pipe (6).

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