Culture device for stem cells

Abstract

The invention provides a stem cell culture device which comprises a culture box body, a top shell fixedly mounted at the top of the culture box body, a controller fixedly mounted on one side of the culture box body and a rotating arm movably mounted in the culture box body, and a heating mounting plate is fixedly mounted at the bottom of the inner wall of the culture box body. The stem cells can be rapidly transferred to temperature positions adaptive to different stages, the stem cells in different stages can be cultured at the same time, the stem cells in different stages can be cultured synchronously, and the adaptive transfer temperature is ensured during transfer, so that the problems that the temperature is not proper in the culture process of an existing temperature box, and the culture efficiency is low are solved. The stem cells in the vessel cannot rapidly obtain the required culture temperature, the required temperatures are different when the stem cells are not in the same culture stage, it cannot be guaranteed that the stem cells which are just added in different stages can be directly placed at the culture positions with the required temperatures, and the problem that the stem cells are taken out, temporarily stored and transferred after cell culture does not exist.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, and in particular to a culture device for stem cells. Background Art

[0002] Stem cells are a type of cell with the potential for self-renewal and multidirectional differentiation, and have shown great application potential in many fields such as medical research, disease treatment, and regenerative medicine. With the continuous deepening of stem cell research, the demand for efficient and precise culture devices that match them has become increasingly prominent.

[0003] In recent years, stem cell research has made many breakthroughs. For example, in the field of regenerative medicine, stem cells are expected to be used to repair damaged tissues and organs, such as using mesenchymal stem cells to treat osteoarthritis, myocardial infarction and other diseases; in drug development, stem cells can be used as in vitro models to screen and evaluate the effectiveness and safety of drugs, which can more accurately reflect the effects of drugs on human cells than traditional animal models. The expansion of these application scenarios has led to a sharp increase in the demand for large quantities of high-quality, functionally stable stem cells.

[0004] In the existing technology, the stem cell culture device has the following technical problems: When the temperature is not suitable during the culture process in a uniform temperature box, the stem cells in the vessel cannot quickly reach the required culture temperature; When multiple stem cells are cultured at the same time, if they are not in the same culture stage, the required temperature is different, and it cannot be guaranteed that the newly added stem cells at different stages can be directly placed in the required temperature culture position; There is no temporary transfer position for taking out cells after culture. When taking out cells, cooling will occur, which will affect the vitality of cell culture, causing an imbalance in internal and external ion concentrations and reducing the survival rate. Summary of the invention

[0005] In view of the deficiencies in the prior art, the purpose of the present invention is to provide a stem cell culture device, which can quickly transfer stem cells to temperature positions suitable for different stages, can culture stem cells at different stages at the same time, can culture stem cells at different stages synchronously and ensure that the transfer temperature is suitable, so as to solve the problem that when the temperature is not suitable during the culture process of the existing temperature box, the stem cells in the vessel cannot quickly obtain the required culture temperature, and the required temperatures are different when they are not in the same culture stage. It cannot be guaranteed that the newly added stem cells at different stages can be directly placed at the required temperature culture position, and there is no temporary transfer position for taking out the cells after culture.

[0006] In order to achieve the above-mentioned purpose, the present invention is realized by the following technical scheme: a culture device for stem cells, comprising a culture box, a top shell fixedly mounted on the top of the culture box, a controller fixedly mounted on one side of the culture box, and a rotating arm movably mounted inside the culture box, a heating mounting plate fixedly mounted on the bottom of the inner wall of the culture box, a culture partition located at the bottom of the rotating arm and at the top of the heating mounting plate fixedly mounted inside the culture box, eight groups of culture dish placement holes distributed in an annular manner are opened around the top of the culture partition, and the tops of the eight groups of culture dish placement holes are all provided with sealing covers that are sealed therewith, a transfer hole is opened at the center of the top of the culture partition, a cell culture dish is arranged inside the culture dish placement hole, an electric heating plate located at the bottom of the culture dish placement hole and the transfer hole is fixedly mounted inside the heating mounting plate, and also includes a culture dish conversion mechanism fixedly mounted on the top of the inner wall of the top shell, and the culture dish conversion mechanism is used to drive the rotating arm to rotate inside the culture box to determine the position where the culture dish needs to be converted; The vessel pressing and extracting mechanism is slidably mounted inside the rotating arm and is used to press against the culture vessel to perform extraction and placement operations; A transverse driving mechanism is slidably mounted inside the rotating arm and is used to drive the drive vessel against the extraction mechanism for displacement; The synchronous cover flipping driving mechanism is fixedly mounted on the bottom of the rotating arm on one side close to the inner wall of the incubator, and is used for driving the sealing cover to flip open while rotating with the rotating arm.

[0007] Furthermore, the culture dish conversion mechanism includes a top frame, a reinforcement plate, a stabilizing frame and a servo motor. The top frame is provided with four groups and is annularly distributed on the top of the inner wall of the top shell and fixedly installed on the top shell. The reinforcement plate is fixedly installed on the bottom of the four groups of top frames. The stabilizing frame is rotatably installed on the bottom of the reinforcement plate. The servo motor is fixedly installed at the center of the top of the inner wall of the top shell. The output end of the servo motor passes through the reinforcement plate and extends to the top of the stabilizing frame and is fixedly installed on the stabilizing frame.

[0008] Furthermore, the vessel pressing and extracting mechanism includes an electric push rod 1, a lifting rod, a fixing frame, an electric push rod 2, a pressing plate and an expansion assembly, wherein the electric push rod 1 is slidably installed inside the rotating arm, the lifting rod is fixedly installed at the output end of the electric push rod 1, the fixing frame is sleeved and fixed on the top of the lifting rod surface, the electric push rod 2 is fixedly installed on the side of the fixing frame away from the lifting rod, the pressing plate is slidably sleeved on the bottom of the lifting rod surface, the top of the pressing plate is fixedly installed on the output end of the electric push rod 2, and the expansion assembly is arranged at the bottom of the lifting rod and is used to tension the cell culture dish.

[0009] Furthermore, the expansion assembly includes a clamping ring, a connecting ring, a pushing ring frame, a scissors-type telescopic frame, a fixing seat, a tensioning plate and a second spring, the clamping ring being slidably mounted on the surface of the lifting rod and fixedly mounted to the bottom of the clamping plate, the connecting ring being detachably mounted on the bottom of the clamping ring by bolts, the pushing ring frame being rotatably sleeved on the top of the outer surface of the connecting ring, the scissors-type telescopic frame being provided with three groups, and the tops of the three groups of scissors-type telescopic frames on the side close to the pushing ring frame are hinged to the pushing ring frame, the tops of the scissors-type telescopic frames on the side away from the pushing ring frame are slidably mounted with the tensioning plate, the bottoms of the scissors-type telescopic frames on the side away from the pushing ring frame are fixedly hinged with the bottoms of the tensioning plate on the side close to the lifting rod, the bottoms of the scissors-type telescopic frames on the side close to the lifting rod are hinged to the fixing seat, the second spring is sleeved on the surface of the lifting rod, the top of the second spring contacts the bottom of the connecting ring, and the bottom of the second spring is fixedly mounted to the top of the fixing seat.

[0010] Furthermore, the lateral driving mechanism includes a linear motor stator, a linear motor mover and a driving frame, the linear motor stator is fixedly mounted on the back of the rotating arm, the linear motor mover is mounted on the surface of the linear motor stator, the driving frame is fixedly mounted on the top of the linear motor mover, the driving frame is fixedly mounted on the bottom of the electric push rod 1, and the output end of the electric push rod 1 passes through the driving frame and cooperates with the lifting rod transmission.

[0011] Furthermore, the synchronous flap driving mechanism comprises an annular lifting guide frame, a limiting cylinder, a sliding rod, a roller, an arcuate tooth plate, a spring 1 and a power conversion flap assembly, wherein the annular lifting guide frame is fixedly mounted on the top of the culture partition, the limiting cylinder is fixedly mounted on the bottom of the rotating arm on the side close to the inner wall of the culture box body, the sliding rod is slidably mounted inside the limiting cylinder, the roller is rotatably mounted on the bottom of the sliding rod through an axle pin, the arcuate tooth plate is fixedly mounted on the side close to the culture partition at the bottom of the sliding rod, the spring 1 is arranged inside the limiting cylinder, the top of the spring 1 is fixedly mounted on the top of the inner wall of the limiting cylinder, the bottom of the spring 1 is fixedly mounted on the top of the sliding rod, the power conversion flap assembly is arranged on the culture partition, and eight groups of annular groups are arranged on the side of the culture dish placement hole away from the transfer hole, and the power conversion flap assembly is used to convert the torsional force of the rotating arm to flip open the sealing cover for easy placement of the cell culture dish.

[0012] Furthermore, the power conversion flip cover assembly includes a support frame, a vertical pole, a driving gear 1, a bevel gear 1, a bevel gear 2, a transverse driving rod, a driving gear 2, a driving gear 3 and a bracket. The support frame is fixedly mounted on the side of the top of the culture partition away from the transfer hole and close to the sealing cover, the vertical pole is longitudinally rotatably mounted on one side of the top of the support frame, the driving gear 1 is fixedly mounted on the top of the vertical pole and meshes with the arc-shaped toothed plate, the bevel gear 1 is fixedly mounted on the bottom of the surface of the vertical pole, the transverse driving rod is transversely rotatably mounted inside the support frame, the bevel gear 2 is fixedly mounted on the side of the transverse driving rod close to the bevel gear 1 and meshes with the bevel gear 1, the driving gear 2 is fixedly mounted on the side of the transverse driving rod away from the bevel gear 2, the bracket is fixedly mounted on the top of the culture partition and located on both sides between the support frame and the sealing cover, the driving gear 3 is fixedly mounted on the side of the sealing cover close to the driving gear 2, the driving gear 3 meshes with the driving gear 2, and the side of the sealing cover close to the support frame is rotationally matched with the bracket through an axle pin.

[0013] Furthermore, an inspection plate is detachably mounted on the bottom of one side of the culture box body, and the four corners of the inspection plate are magnetically fixed to the culture box body through magnetic blocks.

[0014] Furthermore, a temperature sensor is fixedly installed at the bottom of the sealing cover, and limiting convex rings are fixedly installed inside the culture dish placement hole and the transfer hole, and the cell culture dish is longitudinally limited.

[0015] The beneficial effects of the present invention are as follows: the present invention transfers vessels that need to be transferred and the culture temperature needs to be quickly changed through the transfer hole, and the culture dish conversion mechanism drives the rotating arm to rotate, so that the rotating arm cooperates with the lateral driving mechanism to drive the vessel against the extraction mechanism, so that the cell culture dish is extracted and moved to the culture dish placement hole with the required temperature, and cooperates with the temperature-adaptive electric heating plate to carry out temperature culture of the cell culture dish at the corresponding stage. At the same time, when the rotating arm is flipped, the synchronous flip cover driving mechanism can open the sealing cover corresponding to the position that needs to be converted, which is convenient for extraction conversion operation, and improves the conversion efficiency and temperature adaptability of cell culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Other features, objects and advantages of the present invention will become more apparent from the detailed description of non-limiting embodiments made with reference to the following drawings: Figure 1 It is a structural schematic diagram of the present invention; Figure 2 It is a schematic diagram of a half-cut three-dimensional structure of the present invention; Figure 3 It is a schematic diagram of the explosion structure of the culture partition of the present invention; Figure 4 It is a schematic diagram of the three-dimensional structure of the sliding rod of the present invention; Figure 5 For the present invention Figure 4 Another perspective structural diagram of; Figure 6 It is a schematic diagram of the exploded three-dimensional structure of the rotating arm of the present invention; Figure 7 It is a schematic diagram of the exploded three-dimensional structure of the tensioning plate of the present invention.

[0017] In the figure: 1, incubator; 11, top shell; 12, controller; 13, inspection panel; 2, rotating arm; 201, top frame; 202, reinforcement plate; 203, stabilizing frame; 204, servo motor; 21, limit cylinder; 211, sliding rod; 212, roller; 213, arc tooth plate; 214, spring 1; 22, linear motor stator; 221, linear motor mover; 222, driving frame; 223, electric push rod 1; 224, lifting rod; 2241, fixing frame; 2242, electric push rod 2; 2243, tightening plate; 2244, tightening ring; 22441, connecting ring; 22442, pushing ring Frame; 22443, scissor-type telescopic frame; 22444, fixed seat; 22445, tensioning plate; 22446, spring two; 3, culture partition; 31, annular lifting guide frame; 32, transfer hole; 33, limiting convex ring; 34, culture dish placement hole; 35, cell culture dish; 301, sealing cover; 3011, temperature sensor; 341, support frame; 342, vertical pole; 343, driving gear one; 344, bracket; 345, bevel gear one; 346, bevel gear two; 347, horizontal driving rod; 348, driving gear two; 349, driving gear three; 4, heating mounting plate; 41, electric heating plate. DETAILED DESCRIPTION

[0018] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further explained below in conjunction with specific implementation methods.

[0019] See also Figure 1 and Figure 2 , Figure 1 It is a structural schematic diagram of the present invention; Figure 2 It is a schematic diagram of the half-section three-dimensional structure of the present invention.

[0020] A stem cell culture device, comprising a culture box 1, a top shell 11 fixedly mounted on the top of the culture box 1, a controller 12 fixedly mounted on one side of the culture box 1, and a rotating arm 2 movably mounted inside the culture box 1, a heating mounting plate 4 fixedly mounted on the bottom of the inner wall of the culture box 1, a culture partition 3 located at the bottom of the rotating arm 2 and at the top of the heating mounting plate 4 fixedly mounted inside the culture box 1, eight groups of culture dish placement holes 34 distributed in an annular manner are opened around the top of the culture partition 3, and the tops of the eight groups of culture dish placement holes 34 are all provided with sealing members that seal with them Cover 301, a transfer hole 32 is opened at the top center of the culture partition 3, a cell culture dish 35 is arranged inside the culture dish placement hole 34, and an electric heating plate 41 is fixedly installed inside the heating installation plate 4 at the bottom of the culture dish placement hole 34 and the transfer hole 32. The electric heating plate 41 is electrically connected to the controller 12, and the controller 12 is used to control the temperature of the electric heating plate 41; it also includes a culture dish conversion mechanism fixedly installed on the top of the inner wall of the top shell 11, and the culture dish conversion mechanism is used to drive the rotating arm 2 to rotate inside the culture box 1 to determine the position where the culture vessel needs to be converted.

[0021] See also Figures 2 to 7 As shown, Figure 2 It is a schematic diagram of a half-cut three-dimensional structure of the present invention; Figure 3 It is a schematic diagram of the explosion structure of the culture partition of the present invention; Figure 4 It is a schematic diagram of the three-dimensional structure of the sliding rod of the present invention; Figure 5 For the present invention Figure 4 Another perspective structural diagram of; Figure 6 It is a schematic diagram of the exploded three-dimensional structure of the rotating arm of the present invention; Figure 7 It is a schematic diagram of the exploded three-dimensional structure of the tensioning plate of the present invention.

[0022] The culture dish conversion mechanism includes a top frame 201, a reinforcement plate 202, a stabilizing frame 203 and a servo motor 204. The top frame 201 is provided with four groups and is annularly distributed at the top of the inner wall of the top shell 11 and fixedly installed with the top shell 11. The reinforcement plate 202 is fixedly installed at the bottom of the four groups of top frames 201. The stabilizing frame 203 is rotatably installed at the bottom of the reinforcement plate 202. The servo motor 204 is fixedly installed at the center of the top of the inner wall of the top shell 11. The output end of the servo motor 204 penetrates the reinforcement plate 202 and extends to the top of the stabilizing frame 203 and is fixedly installed with the stabilizing frame 203. The top frame 201 is convenient for fixing the reinforcement plate 202 on the top shell 11, and then the stabilizing frame 203 that is rotatably matched with the reinforcement plate 202 can make the rotating arm 2 have anti-bending force when rotating. Starting the servo motor 204 can penetrate the reinforcement plate 202 through the output end to drive the stabilizing frame 203 to rotate, and then the stabilizing frame 203 drives the rotating arm 2 to rotate synchronously, so that it rotates to the position required for conversion.

[0023] The vessel pressing and extracting mechanism is slidably installed inside the rotating arm 2, and is used to press against the culture vessel for extraction and placement operations; the vessel pressing and extracting mechanism includes an electric push rod 1 223, a lifting rod 224, a fixing frame 2241, an electric push rod 2242, a pressing plate 2243 and an expansion component. The electric push rod 1 223 is slidably installed inside the rotating arm 2, the lifting rod 224 is fixedly installed at the output end of the electric push rod 1 223, the fixing frame 2241 is sleeved and fixed on the top of the surface of the lifting rod 224, the electric push rod 2242 is fixedly installed on the side of the fixing frame 2241 away from the lifting rod 224, the pressing plate 2243 is slidably sleeved on the bottom of the surface of the lifting rod 224, the top of the pressing plate 2243 is fixedly installed on the output end of the electric push rod 2242, and the expansion component is arranged at the bottom of the lifting rod 224 and is used to tension the cell culture dish 35. When the electric push rod 1 223 is started, it can drive the lifting rod 224 to retract up and down through the output end. When the lifting rod 224 is extended and retracted up and down, it can drive the fixed frame 2241 and the expansion component at the bottom to move up and down synchronously. At the same time, when the electric push rod 2242 needs to be tensioned, it can drive the clamping plate 2243 to move downward, and drive the expansion component to expand through the clamping plate 2243 to tension the cell culture dish 35 for easy extraction and placement operations.

[0024] The expansion assembly includes a tightening ring 2244, a connecting ring 22441, a pushing ring frame 22442, a scissor-type telescopic frame 22443, a fixing seat 22444, a tensioning plate 22445 and a spring 22446. The tightening ring 2244 is slidably mounted on the surface of the lifting rod 224 and fixedly mounted on the bottom of the tightening plate 2243. The connecting ring 22441 is detachably mounted on the bottom of the tightening ring 2244 by bolts. The pushing ring frame 22442 is rotatably sleeved on the top of the outer surface of the connecting ring 22441. The scissor-type telescopic frame 22443 is provided with three groups, and the top of the three groups of scissor-type telescopic frames 22443 close to the pushing ring frame 22442 is connected to the top of the side of the lifting rod 22444. The pushing ring frame 22442 is hinged, the top of the scissor-type telescopic frame 22443 away from the pushing ring frame 22442 is slidably installed with the tensioning plate 22445, the bottom of the scissor-type telescopic frame 22443 away from the pushing ring frame 22442 is fixedly hinged with the bottom of the tensioning plate 22445 close to the lifting rod 224, the bottom of the scissor-type telescopic frame 22443 close to the lifting rod 224 is hinged to the fixed seat 22444, the spring 22446 is sleeved on the surface of the lifting rod 224, the top of the spring 22446 is in contact with the bottom of the connecting ring 22441, and the bottom of the spring 22446 is fixedly installed with the top of the fixed seat 22444.

[0025] When the clamping plate 2243 is driven downward by the electric push rod 2242, the clamping ring 2244 can be driven to move downward synchronously, and then the clamping ring 22442 is pushed downward by the clamping ring 2244, and then the ring frame 22442 is pushed to move closer to each other through the hinged scissor-type telescopic frame 22443 on the top and bottom of the side close to the lifting rod 224, and then the tensioning plate 22445 is pushed outward to expand and clamp the cell culture dish 35, so that the cell culture dish 35 can be extracted after being clamped. At the same time, the spring 22446 The downward squeezing force on the connecting ring 22441 squeezes the elastic force to shrink. When it needs to shrink, the output end of the electric push rod 2242 can be started again to drive the clamping plate 2243 to move upward. Then the elastic force of the spring 22446 can be released to drive the pushing ring frame 22442, the connecting ring 22441 and the clamping ring 2244 to move upward synchronously, forcing the two ends of the top of the scissor-type telescopic frame 22443 to approach each other and then drive the tensioning plate 22445 to retract. At this time, the cell culture dish 35 can lose the tightness of the tensioning plate 22445 and is convenient for placement.

[0026] The transverse driving mechanism is slidably mounted inside the rotating arm 2 and is used to drive the cell culture dish against the extraction mechanism for displacement; the transverse driving mechanism includes a linear motor stator 22, a linear motor mover 221 and a driving frame 222, the linear motor stator 22 is fixedly mounted on the back of the rotating arm 2, the linear motor mover 221 is mounted on the surface of the linear motor stator 22, the driving frame 222 is fixedly mounted on the top of the linear motor mover 221, the driving frame 222 is fixedly mounted on the bottom of the electric push rod 223, and the output end of the electric push rod 223 penetrates the driving frame 222 and is in transmission cooperation with the lifting rod 224. After the linear motor stator 22 and the linear motor mover 221 are in transmission cooperation, the linear motor mover 221 can be driven to slide transversely on the linear motor stator 22, and at the same time, it is convenient to drive the driving frame 222 to move transversely through the linear motor mover 221, and then drive the electric push rod 223 to move transversely through the driving frame 222, so that the cell culture dish 35 fixed on the tensioning plate 22445 can be moved to the position where it needs to be transferred.

[0027] The synchronous cover-turning driving mechanism is fixedly mounted on one side of the bottom of the rotating arm 2 close to the inner wall of the culture box body 1, and is used to drive the sealing cover 301 to flip open while following the rotation of the rotating arm 2; the synchronous cover-turning driving mechanism includes an annular lifting guide frame 31, a limiting cylinder 21, a sliding rod 211, a roller 212, an arc-shaped tooth plate 213, a spring 214 and a power conversion cover-turning assembly, the annular lifting guide frame 31 is fixedly mounted on the top of the culture partition 3, the limiting cylinder 21 is fixedly mounted on one side of the bottom of the rotating arm 2 close to the inner wall of the culture box body 1, the sliding rod 211 is slidably mounted inside the limiting cylinder 21, and the roller 212 is driven by the rotating arm 2. The axle pin is rotatably installed at the bottom of the sliding rod 211, the arc-shaped tooth plate 213 is fixedly installed at the bottom of the sliding rod 211 on one side close to the culture partition 3, the spring 214 is arranged inside the limiting cylinder 21, the top of the spring 214 is fixedly installed at the top of the inner wall of the limiting cylinder 21, the bottom of the spring 214 is fixedly installed at the top of the sliding rod 211, the power conversion flap assembly is arranged on the culture partition 3, and eight groups of rings are arranged on the side of the culture dish placement hole 34 away from the transfer hole 32, the power conversion flap assembly is used to convert the torsional force of the rotating arm 2 to open the sealing cover 301 for easy placement of the cell culture dish 35.

[0028] The annular lifting guide frame 31 can provide guidance for the moving trajectory of the roller 212. When the rotating arm 2 rotates, the sliding rod 211 follows the rotation, and then the arc-shaped toothed plate 213 always maintains the downward elastic force on the sliding rod 211, ensuring that the roller 212 at the bottom of the sliding rod 211 contacts the top of the annular lifting guide frame 31, thereby facilitating the roller 212 to change its height according to the slope of the annular lifting guide frame 31. When the roller 212 is at the top position on the annular lifting guide frame 31, the arc-shaped toothed plate 213 and the driving gear 1 343 will not engage. When the roller 212 is at the bottom position on the annular lifting guide frame 31, the arc-shaped toothed plate 213 and the driving gear 1 343 will engage, thereby being able to drive the power conversion flip cover assembly.

[0029] The power conversion flip cover assembly includes a support frame 341, a vertical rod 342, a driving gear 1 343, a bevel gear 1 345, a bevel gear 2 346, a transverse driving rod 347, a driving gear 2 348, a driving gear 3 349 and a bracket 344. The support frame 341 is fixedly mounted on the top of the culture partition 3 on the side away from the transfer hole 32 and close to the sealing cover 301. The vertical rod 342 is longitudinally rotatably mounted on one side of the top of the support frame 341. The driving gear 1 343 is fixedly mounted on the top of the vertical rod 342 and meshes with the arc-shaped toothed plate 213. The bevel gear 1 345 is fixedly mounted on the bottom of the surface of the vertical rod 342. The transverse driving rod 347 is transversely rotatably mounted on the support frame 344. Inside the support frame 341, bevel gear two 346 is fixedly mounted on the side of the transverse driving rod 347 close to bevel gear one 345 and meshes with bevel gear one 345, drive gear two 348 is fixedly mounted on the side of the transverse driving rod 347 away from bevel gear two 346, bracket 344 is fixedly mounted on the top of the culture partition 3 and is located on both sides between the support frame 341 and the sealing cover 301, drive gear three 349 is fixedly mounted on the side of the sealing cover 301 close to drive gear two 348, drive gear three 349 and drive gear two 348 mesh with each other, and the side of the sealing cover 301 close to the support frame 341 is rotatably matched with the bracket 344 through an axle pin.

[0030] When the rotating arm 2 rotates and drives the roller 212 to be at the bottom position on the annular lifting guide frame 31, the arc-shaped toothed plate 213 can drive the driving gear 1 343 to rotate by meshing with the driving gear 1 343. After the driving gear 1 343 rotates, it can drive the bevel gear 1 345 to rotate through the vertical rod 342, and then drive the bevel gear 2 346 to rotate through the bevel gear 1 345. The rotation of the bevel gear 2 346 can drive the horizontal driving rod 347 to rotate, and then drive the driving gear 2 348 to rotate through the horizontal driving rod 347. The driving gear 3 349 is driven to rotate by meshing with the driving gear 2 348 and the driving gear 3 349, and then drives the sealing cover 301 to rotate, so that the sealing cover 301 is flipped upward around the axis of the bracket 344, so that the linear motor stator 22 on the rotating arm 2 drives the linear motor mover 221 to drive the electric push rod 1 223 on the driving frame 222 laterally to achieve the position transfer of the cell culture dish 35.

[0031] A maintenance plate 13 is detachably mounted on the bottom of one side of the culture box 1, and the four corners of the maintenance plate 13 are magnetically fixed to the culture box 1 by magnetic blocks. The maintenance plate 13 can be opened to facilitate the user to access the cell culture dish 35, and is convenient for maintenance. At the same time, the magnetic fixation improves the convenience of fixation during access.

[0032] A temperature sensor 3011 is fixedly installed at the bottom of the sealing cover 301, and a limiting convex ring 33 is fixedly installed inside the culture dish placement hole 34 and the transfer hole 32, and the cell culture dish 35 is longitudinally limited. The temperature sensor 3011 can detect the cultivation temperature of each group of culture dish placement holes 34 to determine the different temperatures of each group. At the same time, the limiting convex ring 33 can facilitate the cell culture dish 35 to not fall after being placed, thereby improving its convenience in use during cultivation.

[0033] Working principle: When multi-stage stem cell cultivation is carried out, the most suitable temperature position can be selected according to the current stage, and the corresponding temperature can be detected by the temperature sensor 3011 of the sealing cover 301 on each group of culture dish placement holes 34 to obtain the most suitable position for the stage, and then the electric heating plate 41 at the transfer hole 32 is adjusted to a suitable transfer temperature. After the transfer temperature is adapted, the servo motor 204 is started to drive the stable frame 203 to rotate through the output end, and then the stable frame 203 drives the rotating arm 2 to rotate synchronously, so that it rotates to a conversion position suitable for the temperature of the current stem cell stage; When the rotating arm 2 rotates, the rotating arm 2 drives the limiting cylinder 21 and the sliding rod 211 sliding inside it to pass through the high and low guide positions of the annular lifting guide frame 31. When it moves to a certain distance before the appropriate position, the sliding rod 211 is driven by the elastic force of the spring 1 214 to make the roller 212 at the low position on the annular lifting guide frame 31, and then the arc-shaped toothed plate 213 on the sliding rod 211 is meshed with the driving gear 1 343. At this time, the rotating arm 2 continues to rotate to align with the required position. At this time, the arc-shaped toothed plate 213 drives the driving gear 1 343 to rotate by meshing with the driving gear 1 343. After the driving gear 1 343 rotates, it can pass through the vertical rod 34 2 drives the bevel gear 1 345 to rotate, and then drives the bevel gear 2 346 to rotate through the bevel gear 1 345. The rotation of the bevel gear 2 346 can drive the horizontal driving rod 347 to rotate, and then drives the driving gear 2 348 to rotate through the horizontal driving rod 347. The driving gear 3 349 is driven to rotate by the meshing of the driving gear 2 348 and the driving gear 3 349, and then drives the sealing cover 301 to rotate, so that the sealing cover 301 is turned upward around the axis of the bracket 344, so that it is convenient for the linear motor stator 22 on the rotating arm 2 to drive the linear motor mover 221 to drive the electric push rod 1 223 on the driving frame 222 to drive it horizontally to the top of the transfer hole 32; Then, starting the electric push rod 1 223 can drive the lifting rod 224 to retract downward through the output end. When the lifting rod 224 is retracted downward, it can drive the fixing frame 2241 and the expansion component at the bottom to move up and down synchronously. At the same time, when the electric push rod 2242 needs to tighten the cell culture dish 35, starting the electric push rod 2242 can drive the clamping plate 2243 to move downward. When the clamping plate 2243 is driven downward by the electric push rod 2242, it can drive the clamping ring 2244 to move downward synchronously, and then the clamping ring 2244 squeezes and pushes the ring frame 22442 to move downward. Then, the ring frame 22442 is pushed to make the top and bottom of the side of the lifting rod 224 approach each other through the hinged scissor-type telescopic frame 22443, and then the tensioning plate 22445 is pushed outward to expand and tighten the cell culture dish 35, so that the cell culture dish 35 can be extracted after being tightened. At the same time, the spring 22446 is squeezed and elastically contracted by the downward squeezing force of the connecting ring 22441. At this time, after the tightening of the cell culture dish 35 is completed, the electric push rod 1 223 is started again to drive the lifting rod 224 to move upward, which can synchronously drive the cell culture dish 35 on the tensioning plate 22445 to move upward. Then, the linear motor mover 221 on the driving linear motor stator 22 moves the driving frame 222 and the electric push rod 1 223 to the top of the culture dish placement hole 34 at the required temperature. Then, the electric push rod 1 223 is started again to move downward, and the cell culture dish 35 is driven downward to the inside of the culture dish placement hole 34 through the linear motor stator 22. At this time, the output end of the electric push rod 2242 is started again to drive the clamping plate 2243 to move upward, and then the elastic force of the spring 22446 is released to drive the pushing ring frame 22442, the connecting ring 22441 and the clamping ring 2244 to move upward synchronously, forcing the two ends of the top of the scissor-type telescopic frame 22443 to approach each other and drive the tensioning plate 22445 to retract. At this time, the cell culture dish 35 can lose the clamping of the tensioning plate 22445 and is convenient for placement. Then, the electric push rod 1 223 is started again to drive the tensioning plate 22445 to retract upward, and then the servo motor 204 is started to drive the stable frame 203 and the rotating arm 2 to rotate to the high position of the annular lifting guide frame 31, so that the arc-shaped toothed plate 213 is separated from the driving gear 1 343, so that the sealing cover 301 is convenient to fall again by its own weight for sealing, or the torsion spring is fixed at the connection between the bracket 344 and the shaft pin of the sealing cover 301 for torsion flip sealing; At the same time, the above steps can also be converted to each other through multiple groups on the culture dish placement holes 34. When the temperature position of the cell culture dish 35 not added from the outside needs to be converted, the above steps can still be used for conversion. The simplified process is as follows: Step 1: Temperature detection and rotating arm positioning; The temperature of each culture dish placement hole 34 is detected by the temperature sensor 3011 at the bottom of the sealing cover 301 to determine the temperature position suitable for the current stem cell stage; Start the servo motor 204 to drive the stabilizing frame 203 and the rotating arm 2 to rotate to corresponding positions; Step 2: Open the sealing cover and prepare for transfer; During the rotation of the rotating arm 2, the arc-shaped tooth plate 213 on the sliding rod 211 is meshed with the driving gear 1 343 through the synchronous cover-turning driving mechanism, driving the sealing cover 301 to flip upward and open; The linear motor stator 22 cooperates with the linear motor mover 221 to drive the electric push rod 223 to move horizontally so as to subsequently transfer the cell culture dish 35; Step 3: Extraction and transfer of cell culture dishes; The electric push rod 1 223 is started to drive the lifting rod 224 to retract downward, and the electric push rod 2242 drives the clamping plate 2243 to move downward, and the cell culture dish 35 is tensioned and extracted through the expansion component; The linear motor stator 22 drives the linear motor mover 221 to drive the electric push rod 1 223 to move horizontally to above the target culture dish placement hole 34; Start the electric push rod 223 again and move it downward to put the cell culture dish 35 into the target culture dish placement hole 34; Step 4: Reset the sealing cover and the device; After the placement is completed, the cell culture dish 35 is easily placed by operating the relevant components in the reverse direction, such as starting the electric push rod 2242 again to retract the expansion assembly. The servo motor 204 is started to drive the rotating arm 2 to rotate to the high position of the annular lifting guide frame 31, so that the arc-shaped tooth plate 213 is separated from the driving gear 1 343, and the sealing cover 301 is reset and sealed.

[0034] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the attached claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention. Any figure mark in the claims should not be regarded as limiting the claims involved.

[0035] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A stem cell culture device, comprising a culture box (1), a top shell (11) fixedly mounted on the top of the culture box (1), a controller (12) fixedly mounted on one side of the culture box (1), and a rotating arm (2) movably mounted inside the culture box (1), wherein a heating mounting plate (4) is fixedly mounted on the bottom of the inner wall of the culture box (1), and a culture partition (3) located at the bottom of the rotating arm (2) and at the top of the heating mounting plate (4) is fixedly mounted inside the culture box (1), wherein the culture partition Eight groups of culture dish placement holes (34) are arranged in an annular manner around the top of the culture partition (3), and the tops of the eight groups of culture dish placement holes (34) are all provided with sealing covers (301) that are sealed therewith. A transfer hole (32) is provided at the center of the top of the culture partition (3), and a cell culture dish (35) is arranged inside the culture dish placement hole (34). An electric heating plate (41) located at the bottom of the culture dish placement hole (34) and the transfer hole (32) is fixedly installed inside the heating installation plate (4), characterized in that: It also includes a culture dish conversion mechanism fixedly mounted on the top of the inner wall of the top shell (11), the culture dish conversion mechanism being used to drive the rotating arm (2) to rotate inside the culture box (1) to determine the position where the culture dish needs to be converted; The vessel pressing and extracting mechanism is slidably mounted inside the rotating arm (2) and is used to press against the culture vessel to perform extraction and placement operations; A transverse driving mechanism, slidably mounted inside the rotating arm (2) and used to drive the drive vessel to move against the extraction mechanism; The synchronous cover flipping driving mechanism is fixedly mounted on a side of the bottom of the rotating arm (2) close to the inner wall of the culture box body (1), and is used to drive the sealing cover (301) to flip open while rotating with the rotating arm (2).

2. A stem cell culture device according to claim 1, characterized in that: The culture dish conversion mechanism comprises a top frame (201), a reinforcement plate (202), a stabilizing frame (203) and a servo motor (204); the top frame (201) is provided with four groups and is annularly distributed on the top of the inner wall of the top shell (11) and fixedly mounted on the top shell (11); the reinforcement plate (202) is fixedly mounted on the bottom of the four groups of the top frames (201); the stabilizing frame (203) is rotatably mounted on the bottom of the reinforcement plate (202); the servo motor (204) is fixedly mounted at the center of the top of the inner wall of the top shell (11); and the output end of the servo motor (204) passes through the reinforcement plate (202) and extends to the top of the stabilizing frame (203) and is fixedly mounted on the stabilizing frame (203).

3. A stem cell culture device according to claim 1, characterized in that: The vessel pressing and extracting mechanism comprises an electric push rod 1 (223), a lifting rod (224), a fixing frame (2241), an electric push rod 2 (2242), a pressing plate (2243) and an expansion component, wherein the electric push rod 1 (223) is slidably mounted inside the rotating arm (2), the lifting rod (224) is fixedly mounted on the output end of the electric push rod 1 (223), the fixing frame (2241) is sleeved and fixed on the top of the surface of the lifting rod (224), the electric push rod 2 (2242) is fixedly mounted on a side of the fixing frame (2241) away from the lifting rod (224), the pressing plate (2243) is slidably sleeved on the bottom of the surface of the lifting rod (224), the top of the pressing plate (2243) is fixedly mounted on the output end of the electric push rod 2 (2242), and the expansion component is arranged at the bottom of the lifting rod (224) and is used to tension the cell culture dish (35).

4. A stem cell culture device according to claim 3, characterized in that: The expansion assembly comprises a clamping ring (2244), a connecting ring (22441), a pushing ring frame (22442), a scissor-type telescopic frame (22443), a fixing seat (22444), a tensioning plate (22445) and a second spring (22446); the clamping ring (2244) is slidably mounted on the surface of the lifting rod (224) and fixedly mounted on the bottom of the clamping plate (2243); the connecting ring (22441) is detachably mounted on the bottom of the clamping ring (2244) by bolts; the pushing ring frame (22442) is rotatably sleeved on the top of the outer surface of the connecting ring (22441); the scissor-type telescopic frame (22443) is provided in three groups, and the top of the three groups of scissor-type telescopic frames (22443) close to the pushing ring frame (22442) are connected to the top of the side of the lifting rod (22444). The pushing ring frame (22442) is hinged, the top of the scissor-type telescopic frame (22443) away from the pushing ring frame (22442) is slidably installed with the tensioning plate (22445), the bottom of the scissor-type telescopic frame (22443) away from the pushing ring frame (22442) is fixedly hinged with the bottom of the tensioning plate (22445) close to the lifting rod (224), the bottom of the scissor-type telescopic frame (22443) close to the lifting rod (224) is hinged with the fixing seat (22444), the second spring (22446) is sleeved on the surface of the lifting rod (224), the top of the second spring (22446) is in contact with the bottom of the connecting ring (22441), and the bottom of the second spring (22446) is fixedly installed with the top of the fixing seat (22444).

5. The stem cell culture device according to claim 3, characterized in that: The transverse drive mechanism comprises a linear motor stator (22), a linear motor mover (221) and a drive frame (222); the linear motor stator (22) is fixedly mounted on the back of the rotating arm (2); the linear motor mover (221) is mounted on the surface of the linear motor stator (22); the drive frame (222) is fixedly mounted on the top of the linear motor mover (221); the drive frame (222) is fixedly mounted on the bottom of an electric push rod (223); and the output end of the electric push rod (223) passes through the drive frame (222) and is transmission-coordinated with a lifting rod (224).

6. The stem cell culture device according to claim 1, characterized in that: The synchronous cover-turning driving mechanism comprises an annular lifting guide frame (31), a limiting cylinder (21), a sliding rod (211), a roller (212), an arc-shaped tooth plate (213), a spring (214) and a power conversion cover-turning assembly, wherein the annular lifting guide frame (31) is fixedly mounted on the top of the culture partition (3), the limiting cylinder (21) is fixedly mounted on the bottom of the rotating arm (2) on a side close to the inner wall of the culture box body (1), the sliding rod (211) is slidably mounted inside the limiting cylinder (21), the roller (212) is rotatably mounted on the bottom of the sliding rod (211) via an axle pin, and the arc-shaped tooth plate (213) is fixedly mounted On the side of the bottom of the sliding rod (211) close to the culture partition (3), the spring 1 (214) is arranged inside the limiting cylinder (21), the top of the spring 1 (214) is fixedly mounted to the top of the inner wall of the limiting cylinder (21), the bottom of the spring 1 (214) is fixedly mounted to the top of the sliding rod (211), the power conversion flap assembly is arranged on the culture partition (3), and eight groups of rings are arranged and distributed on the side of the culture dish placement hole (34) away from the transfer hole (32), the power conversion flap assembly is used to convert the twisting force of the rotating arm (2) to flip open the sealing cover (301) to facilitate the placement of the cell culture dish (35).

7. A stem cell culture device according to claim 6, characterized in that: The power conversion flap assembly comprises a support frame (341), a vertical pole (342), a driving gear 1 (343), a bevel gear 1 (345), a bevel gear 2 (346), a transverse driving rod (347), a driving gear 2 (348), a driving gear 3 (349) and a bracket (344), wherein the support frame (341) is fixedly mounted on a side of the top of the culture partition (3) away from the transfer hole (32) and close to the sealing cover (301), the vertical pole (342) is longitudinally rotatably mounted on one side of the top of the support frame (341), the driving gear 1 (343) is fixedly mounted on the top of the vertical pole (342) and meshes with the arc-shaped tooth plate (213), the bevel gear 1 (345) is fixedly mounted on the bottom of the surface of the vertical pole (342), and the transverse driving rod (347) is transversely rotatably mounted on the top of the culture partition (3). Inside the support frame (341), the bevel gear 2 (346) is fixedly mounted on the side of the transverse driving rod (347) close to the bevel gear 1 (345) and meshes with the bevel gear 1 (345). The drive gear 2 (348) is fixedly mounted on the side of the transverse driving rod (347) away from the bevel gear 2 (346). The support (344) is fixedly mounted on the top of the culture partition (3) and is located on both sides between the support frame (341) and the sealing cover (301). The drive gear 3 (349) is fixedly mounted on the side of the sealing cover (301) close to the drive gear 2 (348). The drive gear 3 (349) and the drive gear 2 (348) are meshed with each other. The side of the sealing cover (301) close to the support frame (341) is rotatably matched with the support (344) through an axle pin.

8. The stem cell culture device according to claim 1, characterized in that: A maintenance plate (13) is detachably mounted on the bottom of one side of the culture box body (1), and the four corners of the maintenance plate (13) are magnetically fixed to the culture box body (1) via magnetic blocks.

9. The stem cell culture device according to claim 1, characterized in that: A temperature sensor (3011) is fixedly mounted on the bottom of the sealing cover (301), and limiting convex rings (33) are fixedly mounted inside the culture dish placement hole (34) and the transfer hole (32), and the cell culture dish (35) is longitudinally limited.

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