Manufacturing method of tissue specimen slice

Abstract

The invention discloses a manufacturing method of a tissue specimen slice. The manufacturing method comprises the following steps: marking information of a tissue specimen on an embedding box; and putting the tissue specimen into the embedding box for embedding. And sequentially carrying out slicing and water bath flattening on the embedded tissue specimen. And acquiring information on the embedding box, and marking the information on the embedding box on the slide. And fishing out the tissue specimen flattened by the water bath onto the slide. According to the manufacturing method disclosed by the invention, the information of the tissue specimens is marked on the embedding box, the information on the embedding box is obtained, and the information on the embedding box is marked on the slide, so that the tissue specimens fished on the slide are in one-to-one correspondence with the embedded tissue specimens, and accidents caused by wearing of the Xiangshan Lie are avoided.

Description

Technical Field

[0001] The invention relates to the field of tissue specimen slice making, and in particular to a method for making tissue specimen slices. Background Art

[0002] In the process of making tissue specimen slices, the tissue transfer process is involved, which is very important. The tissue specimen scooped onto the glass slide and the embedded tissue specimen must correspond one to one, otherwise they will be mixed up and cause accidents. Summary of the invention

[0003] The invention provides a method for making tissue specimen slices, which can ensure that the tissue specimen scooped out on a glass slide and the embedded tissue specimen correspond one to one.

[0004] The embodiment of the present invention provides a method for making tissue specimen sections, comprising the following steps: marking the information of the tissue specimen on an embedding box. Putting the tissue specimen into the embedding box for embedding. Slicing the embedded tissue specimen in sequence and flattening it in a water bath. Obtaining the information on the embedding box, and marking the information on the embedding box on a glass slide. Scooping the tissue specimen flattened in the water bath onto a glass slide.

[0005] In some embodiments, marking the information of the tissue specimen on the embedding cassette includes: inputting the information of the tissue specimen into the system to form a two-dimensional code, and printing the two-dimensional code on the embedding cassette.

[0006] In some embodiments, obtaining the information on the embedding cassette includes: reading the information on the embedding cassette using a barcode scanner.

[0007] In some embodiments, marking the information on the embedding box onto the glass slide includes: forming a two-dimensional code from the information read by the scanner through system processing, and printing the two-dimensional code on the glass slide.

[0008] In some embodiments, before the tissue specimen is placed in an embedding box for embedding, information of the tissue specimen is marked on a dehydration box, and the tissue specimen is placed in the dehydration box for dehydration.

[0009] In some embodiments, marking the information of the tissue specimen on the dehydration box includes: inputting the information of the tissue specimen into the system to form a QR code, and printing the QR code on the dehydration box.

[0010] In some embodiments, the dehydration cassette and the embedding cassette are the same cassette body.

[0011] In some embodiments, the tissue specimen is fixed with a fixative before being placed in a dehydration box for dehydration.

[0012] In some of the embodiments, the tissue specimen is placed in an embedding cassette for embedding: the tissue specimen in the embedding cassette is wrapped with melted paraffin and then frozen to solidify the melted paraffin.

[0013] In some embodiments, after the tissue specimen flattened in a water bath is scooped onto a glass slide, the glass slide with the tissue specimen is baked, and then the tissue specimen on the glass slide is dewaxed and stained in sequence.

[0014] In some embodiments, a barcode scanner for obtaining information on an embedding box includes a housing, a barcode scanner device, and a connector. The housing is provided with a barcode scanner window and a through port. The barcode scanner device is disposed in the housing, and a lens of the barcode scanner device is disposed corresponding to the barcode scanner window for realizing a barcode scanner function. The first end of the connector is disposed in the housing and electrically connected to the barcode scanner device, and the second end of the connector extends out of the housing through the through port for plugging with a power supply connector of an external device, so that the barcode scanner is fixed to the external device through the connector and is powered by the external device. The barcode scanner window and the connector are respectively disposed on adjacent sides of the housing, so that when the barcode scanner is fixed on the four sides of the external device on a vertical plane, the barcode scanner window can be disposed toward the front of the external device. The connector is rotatably plugged with the power supply connector, so that the barcode scanner can be rotated and the direction of the barcode scanner window can be adjusted.

[0015] According to an embodiment of the present invention, a method for making tissue specimen sections includes the following steps: marking the information of the tissue specimen on an embedding box. Putting the tissue specimen into an embedding box for embedding. Slicing the embedded tissue specimen in sequence and flattening it in a water bath. Obtaining the information on the embedding box, and marking the information on the embedding box on a glass slide. Fishing the tissue specimen flattened in a water bath onto a glass slide. The making method of the present invention ensures that the tissue specimen fished onto the glass slide corresponds to the embedded tissue specimen in a one-to-one manner by marking the information of the tissue specimen on the embedding box, and obtaining the information on the embedding box, and marking the information on the embedding box on the glass slide, thereby avoiding accidents caused by misidentification. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without paying any creative work.

[0017] Figure 1 It is a schematic diagram of the process of making tissue specimen slices in an embodiment of the present invention; Figure 2 It is a schematic diagram of the overall structure of the barcode scanner in an embodiment of the present invention; Figure 3It is a schematic diagram of the structure explosion of the barcode scanner at a first angle in an embodiment of the present invention; Figure 4 Schematic diagram of the structure explosion of the barcode scanner at a second angle in an embodiment of the present invention. DETAILED DESCRIPTION

[0018] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0019] See also Figure 1 The embodiment of the present invention provides a method for preparing tissue specimen slices, comprising the following steps: (1) Obtain tissue specimens.

[0020] In the above steps, tissue specimens include but are not limited to animal and plant tissue specimens.

[0021] (2) Fix the tissue specimen using fixative.

[0022] The above steps can be omitted if the tissue specimen is used promptly.

[0023] (3) Label the tissue specimen information on the dehydration box. Label the tissue specimen information on the embedding box.

[0024] In the above steps, the information of the tissue specimen is input into the system to form a QR code, and the QR code is printed on the dehydration box to mark the information of the tissue specimen on the dehydration box. The information of the tissue specimen is input into the system to form a QR code, and the QR code is printed on the embedding box to mark the information of the tissue specimen on the embedding box. The embedding box and the dehydration box can be the same box body.

[0025] (4) Place the tissue specimen in a dehydration box for dehydration.

[0026] In the above steps, when dehydrating, it is necessary to soak in various reagents for about 12 hours.

[0027] (5) Place the dehydrated tissue specimen into an embedding box for embedding.

[0028] In the above steps, the dehydrated tissue specimen is transferred from the dehydration box to the embedding box, and then the tissue specimen in the embedding box is wrapped with melted paraffin and then frozen to solidify the melted paraffin.

[0029] (6) Slice the embedded tissue specimens and flatten them in a water bath.

[0030] In the above steps, the paraffin-embedded tissue specimen is sliced, wherein the tissue specimen is sliced ​​into about 3 um thickness.

[0031] (7) Obtain the information on the embedding box and mark it on the glass slide.

[0032] In the above steps, the information on the embedding box is read by a barcode scanner, and then the system processes the information read by the barcode scanner to form a two-dimensional code, and the two-dimensional code is printed on the slide. Among them, the information on the embedding box can be read by the barcode scanner before sectioning.

[0033] Among them, see Figure 2-4 The code scanner 1 may include a base 11, a circuit board 14, a code scanning device 12 and a connector 13.

[0034] The base 11 is used for arranging various components of the barcode scanner 1. The base 11 may be a shell with a receiving space. The shell may include a box body 111 and a cover body 112.

[0035] One side of the box body 111 may have an opening, in which case the wall of the box body 111 adjacent to the opening is the side of the box body 111, and the wall of the box body 111 opposite to the opening is the bottom of the box body 111. Among them, the box body 111 may be a rectangular parallelepiped with one side open. A code scanning window 113 may be provided on one side of the box body 111, and the code scanning window 113 is used to expose the lens of the code scanning device 12 in the box body 111. The code scanning window 113 may be rectangular. A screw column 114 may be fixed on the inner side of the bottom of the box body 111, and a screw hole is drilled on the screw column 114, and the screw hole is used to fix the circuit board 14 in the box body 111. The screw column 114 may be perpendicular to the bottom of the box body 111. The number of screw columns 114 may be four, and the four screw columns 114 may be respectively arranged at the four corners of the square structure. The center line of the screw hole may coincide with the center line of the screw column 114.

[0036] The cover body 112 is covered and fixed on the opening of the box body 111. The cover body 112 may be in the shape of a flat plate. A through hole 115 may be provided on the cover body 112, and the through hole 115 is used to allow the connector 13 to pass through the inside and outside of the shell. A speaker hole 116 may also be provided on the cover body 112, and the speaker hole 116 is used to transmit the scanning sound of the scanning device 12 in the box body 111. A support rod 117 may be fixed on the side of the cover body 112 facing the box body 111, and the support rod 117 is used to press the circuit board 14 in the box body 111. The support rod 117 may be perpendicular to the cover body 112. There may be two support rods 117, and the two support rods 117 may be respectively arranged on both sides of the square structure where the four screw columns 114 are located.

[0037] The circuit board 14 is used for setting the electronic components of the barcode scanner 1. The circuit board 14 can be set in the box body 111. The circuit board 14 can be fixed on the bottom of the box body 111. The circuit board 14 can be perpendicular to the center line of the opening of the box body 111. The circuit board 14 can be provided with a screw hole 141, and the screw hole 141 is set corresponding to the screw hole, and the screw hole 141 is used to fix the circuit board 14. The number of screw holes 141 can be four, and the four screw holes 141 can be set corresponding to the four screw holes respectively. The side of the circuit board 14 facing the opening of the box body 111 can conflict with the support rod 117.

[0038] The code scanning device 12 is used to realize the code scanning function. The code scanning device 12 is arranged on the base 11. The code scanning device 12 can be arranged in the box body 111. The code scanning device 12 can be fixed on the open side of the circuit board 14 facing the box body 111. The code scanning device 12 is electrically connected to the circuit board 14. The lens of the code scanning device 12 is arranged corresponding to the code scanning window 113.

[0039] The connector 13 is used to be plugged into the power connector of an external device (such as a slicing device) so that the barcode scanner 1 is fixed to the external device through the connector 13 and powered by the external device. The connector 13 is arranged on the base 11. The connector 13 is electrically connected to the barcode scanning device 12. Among them, the connector 13 and the barcode scanning window 113 can be respectively arranged on the adjacent two sides of the base 11 so that the barcode scanner 1 is fixed on the four sides of the external device on the vertical plane. For example, when the barcode scanner 1 is fixed on the left side of the external device, the barcode scanning window 113 can be arranged toward the front of the external device. The connector 13 is rotatably plugged into the power connector, that is, after the connector 13 is plugged into the power connector, the connector 13 and the power connector can be relatively rotated under the drive of an external force, so that the barcode scanner 1 can be rotated, and the direction of the barcode scanning window 113 can be adjusted. For example, the barcode scanner 1 is manually twisted to adjust the barcode scanning window 113 to face the front lower side of the external device, thereby facilitating the barcode scanning operation. The first end of the connector 13 can be arranged in the housing. The first end of the connector 13 can be fixed on the open side of the circuit board 14 facing the box body 111. The first end of the connector 13 can be electrically connected to the circuit board 14. There can be a gap between the first end of the connector 13 and the code scanning device 12. The gap can be set corresponding to the speaker hole 116. The second end of the connector 13 can extend to the outside of the housing through the through hole 115 for plugging with the power supply connector.

[0040] The second end of the connector 13 may be a convex structure so that the connector 13 can be plugged into the power connector of the concave hole structure. The second end of the connector 13 may be a round rod so that the connector 13 can be rotatably plugged into the round hole-shaped power connector. Of course, the second end of the connector 13 may also be other shapes, which will not be described in detail. Under the above conditions, the second end of the connector 13 can extend outside the housing and be a certain distance away from the outside of the housing, so as to facilitate the plugging of the connector 13 with the power connector.

[0041] The second end of the connector 13 may also be a concave hole structure so that the connector 13 can be plugged into the power connector of the convex structure. The second end of the connector 13 may be a circular hole so that the connector 13 can be rotatably plugged into the round rod-shaped power connector. Of course, the second end of the connector 13 may also be other shapes, which will not be described in detail. Under the above conditions, the second end of the connector 13 can just extend to the outside of the housing, thereby optimizing the appearance.

[0042] (8) Place the tissue specimen that has been flattened in the water bath onto a glass slide.

[0043] In the above steps, the tissue specimen scooped out onto the glass slide corresponds to the embedded tissue specimen one by one.

[0044] (9) Bake the slides with tissue specimens, and then dewax and stain the tissue specimens on the slides in turn.

[0045] In the above steps, the tissue specimen sections obtained after staining can be analyzed under a microscope.

[0046] The preparation method of the present invention ensures that the tissue specimen scooped out on the glass slide and the embedded tissue specimen correspond one to one by marking the information of the tissue specimen on the embedding box, and obtaining the information on the embedding box and marking the information on the embedding box on the glass slide, thereby avoiding accidents caused by misidentification.

[0047] The same or similar reference numerals in the drawings of this embodiment correspond to the same or similar components. In the description of the present invention, it should be understood that if the terms "upper", "lower", "left", "right" and the like indicate an orientation or positional relationship based on the orientation or positional relationship shown in the drawings, it is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, the terms describing the positional relationship in the drawings are only used for exemplary description and cannot be understood as a limitation of the present invention. For ordinary technicians in this field, the specific meanings of the above terms can be understood according to specific circumstances.

[0048] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for preparing tissue specimen sections, characterized in that: The following steps are involved: Label the tissue specimen information on the embedding cassette; Putting the tissue specimen into the embedding box for embedding; The embedded tissue specimens are sequentially sliced ​​and flattened in a water bath; Obtaining information on the embedding box, and marking the information on the embedding box on the glass slide; The tissue specimen flattened in a water bath is scooped onto the glass slide.

2. The method according to claim 1, characterized in that: Label the tissue specimen information on the embedding cassette, including: The information of the tissue specimen is input into the system to form a two-dimensional code, which is then printed on the embedding cassette.

3. The method according to claim 2, characterized in that: Obtaining information on the embedding cassette, including: The information on the embedding box is read by a barcode scanner.

4. The method according to claim 3, characterized in that: The information on the embedding cassette is marked on the slide, including: Through system processing, the information read by the scanner is formed into a two-dimensional code, and the two-dimensional code is printed on a glass slide.

5. The method according to claim 1, characterized in that: Before the tissue specimen is placed in the embedding box for embedding, the information of the tissue specimen is marked on the dehydration box, and the tissue specimen is placed in the dehydration box for dehydration.

6. The method according to claim 5, characterized in that: Label the information of the tissue specimen on the dehydration box, including: The information of the tissue specimen is input into the system to form a QR code, and the QR code is printed on the dehydration box.

7. The method according to claim 5, characterized in that: The dehydration box and the embedding box are the same box body.

8. The method according to claim 5, characterized in that: Before the tissue specimen is placed in the dehydration box for dehydration, a fixative is used to fix the tissue specimen.

9. The method according to claim 1, characterized in that: The tissue specimen is placed in the embedding box for embedding, comprising: Wrapping the tissue specimen in the embedding box with melted paraffin, and then freezing it to solidify the melted paraffin; After the tissue specimen flattened in a water bath is scooped onto the glass slide, the glass slide with the tissue specimen is baked, and then the tissue specimen on the glass slide is dewaxed and stained in sequence.

10. The method according to claim 1, characterized in that: The barcode scanner for obtaining the information on the embedding cassette comprises: The housing is provided with a code scanning window and a through hole; A code scanning device is arranged in the housing, and a lens of the code scanning device is arranged corresponding to the code scanning window, so as to realize a code scanning function; A connector, a first end of which is disposed in the housing and electrically connected to the code scanning device, and a second end of which extends through the through hole to the outside of the housing and is used to be plugged into a power supply connector of an external device, so that the code scanning device is fixed to the external device through the connector and is powered by the external device; The code scanning window and the connector are respectively arranged on two adjacent sides of the housing, so that when the code scanner is fixed around the external device on a vertical plane, the code scanning window can be arranged toward the front of the external device; The connector is rotatably plugged into the power supply connector, so that the barcode scanner can be rotated and the direction of the barcode scanning window can be adjusted.

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