Hydrogel for promoting generation of collagen

Abstract

The invention discloses a hydrogel for promoting collagen generation, which is characterized in that chitosan (natural high-molecular polymer) and polyvinyl alcohol (synthetic high-molecular polymer) are used as raw materials, and a composite high-molecular material is constructed by combining the two raw materials. The invention relates to the technical field of biological medicines. The hydrogel has the functions of promoting the generation of collagen, collagen fibers and new vessels.

Description

Technical Field

[0001] The present invention relates to the field of biomedical technologies, and particularly relates to a hydrogel for promoting collagen production. Background Art

[0002] In recent years, hydrogels have received extensive attention due to their good hydrophilicity, safety, and biocompatibility. A hydrogel is a three-dimensional network hydrophilic material composed of cross-linked polymers and is widely used in medical fields such as drug and gene delivery platforms, wound dressings, and tissue engineering scaffolds. Among them, the metal nanoparticle-hydrogel composite combines the unique properties of noble metal nanoparticles such as corrosion resistance, oxidation resistance, and high surface-to-volume ratio with the porous structure of the hydrogel innovatively. Hydrogel composites with uniformly dispersed and loaded AgNPs have been widely used in clinical research. AgNPs have local surface plasmons, and there is a correlation between its size and the mechanical properties of the hydrogel. Therefore, an efficient green synthesis method is sought to prepare silver nanoparticle-hydrogel bioactive composites to provide a treatment basis for the repair of dermal wounds. Summary of the Invention

[0003] The object of the present invention is to provide a hydrogel for promoting collagen production, using chitosan and polyvinyl alcohol as raw materials for the hydrogel, combining natural polymer and synthetic polymer to construct a composite polymer material hydrogel.

[0004] To achieve the above object, the present invention adopts the following technical solutions:

[0005] The hydrogel provided by the present invention adds AgNPs with excellent antibacterial effects against Gram-positive bacteria, Gram-negative bacteria, and Candida albicans to achieve synergistic antibacterial performance.

[0006] The hydrogel provided by the present invention adds physcion@AgNPs and emodin@AgNPs in a colloidal state with a volume ratio of 5% - 8%.

[0007] The present invention provides a synthesis method for a hydrogel for promoting collagen production.

[0008] The present invention conducts immunofluorescence analysis and immunohistochemical analysis.

[0009] The hydrogels Emodin-AgNPs and Physcion-AgNPs provided by the present invention are compared with the control groups 3M and Gel, and there are differences in the healing process within 12 days after modeling.

[0010] The hydrogel provided by the present invention has the functions of promoting the generation of collagen, collagen fibers, and new blood vessels.

[0011] The present invention also provides an application of synthesizing a hydrogel by the method as described in the present invention, and the hydrogel can be applied to the repair of diabetic wounds, infectious burn wounds, sensitive skin symptoms, etc. Brief Description of the Drawings

[0012] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. It should be understood that the following drawings are only the embodiments of the present invention, and for those of ordinary skill in the art, other drawings can be obtained according to the provided drawings without creative efforts.

[0013] Figure 1 It is the Collal immunofluorescence staining map of the wound tissue obtained in Application Example 2;

[0014] Figure 2 It is the CD31 immunofluorescence staining map of the wound tissue obtained in Application Example 3;

[0015] Figure 3 It is the Masson immunohistochemical staining map of the wound tissue obtained in Application Example 4;

[0016] Figure 4 It is the quantitative analysis map of the Masson immunohistochemical staining obtained in Application Example 4. Detailed Embodiments

[0017] The following are only the preferred embodiments of the present invention, and the protection scope is not limited to this embodiment. All technical solutions falling within the idea of the present invention should belong to the protection scope of the present invention.

[0018] The present invention provides a hydrogel for promoting collagen production, including the following steps:

[0019] Example 1

[0020] A hydrogel for promoting collagen production, and the preparation method is carried out according to the following steps:

[0021] Step 1: Prepare 100 g of an aqueous solution of polyvinyl alcohol with a mass fraction of 10-15%, and stir at high temperature and high speed until the solution is clear and transparent and completely dissolved.

[0022] Step 2: Prepare 100 g of an aqueous solution of chitosan with a mass fraction of 2-5%, and stir at high temperature and high speed until the solution is clear and transparent and completely dissolved.

[0023] Step 3: Prepare a 1 mM emodin / emodin methyl ether solution with ethanol as the solvent, and carry out a 1:1 light reaction with a 10 mM silver nitrate aqueous solution for 48 h to prepare an emodin / emodin methyl ether@AgNPs colloidal state sample.

[0024] Step 4: Mix polyvinyl alcohol and chitosan as a blank hydrogel control group, and simultaneously add emodin methyl ether@AgNPs and emodin@AgNPs (ethanol / aqueous solution) in a colloidal state with a volume ratio of 6%, and perform freeze-thaw crosslinking twice as the colloidal experimental group.

[0025] The present invention also provides the mechanism of action of the hydrogel in the application scenario of infectious wound repair.

[0026] Application Example 1

[0027] In the present invention, male BALB / cJGpt mice adapted to the environment were selected. The experiment was set up with four groups: 3M, Gel, Emodin-AgNPs, and Physcion-AgNPs. 8 mice were taken from each experimental group for wound healing tests. After depilation of each mouse, 2 full-thickness circular parallel skin wounds with a diameter of about 6 mm were created on the back skin, inoculated with 100 μL of Staphylococcus aureus suspension, covered with a hydrogel sample of the same diameter size, and fixed with breathable tape. The wound healing situation was recorded during dressing changes on the 2nd, 4th, 6th, 8th, 10th, 12th, and 14th days after treatment.

[0028] Application Example 2

[0029] In the present invention, on the 12th day after modeling, the wound tissues of the experimental group and the control group were respectively extracted for Collal immunofluorescence staining, and the obtained results are as Figure 1 shown.

[0030] In the present invention, red fluorescence indicates Collal protein expression, and blue fluorescence indicates the cell nucleus.

[0031] It can be seen from the staining results that the red-labeled part representing Collal in the experimental group is more than that in the control group.

[0032] Analysis of the experimental results shows that in the Emodin-AgNPs and Physcion-AgNPs experimental groups, the increased expression of Collal in the hydrogel of the present invention promotes the synthesis and secretion of collagen, indicating that more collagen deposition is beneficial to wound repair.

[0033] Application Example 3

[0034] In the present invention, on the 12th day after modeling, the wound tissues of the experimental group and the control group were respectively extracted for CD31 immunofluorescence staining, and the obtained results are as Figure 2 shown.

[0035] In the present invention, red fluorescence indicates CD31 protein expression, and blue fluorescence indicates the cell nucleus.

[0036] It can be seen from the staining results that the red marker part representing CD31 in the experimental group is more than that in the control group.

[0037] Analysis of the experimental results shows that in the Emodin-AgNPs and Physcion-AgNPs experimental groups, there are more new blood vessels in the skin tissue, indicating that the expression of CD31 in the hydrogel of the present invention promotes angiogenesis, thus facilitating wound repair.

[0038] Application Example 4

[0039] In the present invention, on the 12th day after modeling, the wound tissues of the experimental group and the control group were respectively extracted and subjected to Masson immunohistochemical staining, and the results are as Figure 3 shown.

[0040] In the present invention, it can be seen from the staining results that in the Emodin-AgNPs and Physcion-AgNPs experimental groups, the blue area representing collagen fibers is more than that in the control group, and the results are as Figure 4 shown.

[0041] Analysis of the experimental results shows that it indicates that the hydrogel of the present invention can promote the generation of collagen fibers, improve the microenvironment of the tissue around the wound, and thus accelerate the wound healing process.

[0042] The embodiments of the present invention will be described above with reference to the accompanying drawings and preferred embodiments. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention. It should be understood that the preferred embodiments are only for explaining the present invention, rather than for limiting the protection scope of the present invention.

Claims

1. A hydrogel for promoting collagen production, characterized in that: The steps include: (1) Synthesis of rhein@AgNPs hydrogel (2) Immunofluorescence analysis (3) Immunohistochemical analysis.

2. The synthetic hydrogel according to claim 1, characterized in that Chitosan and polyvinyl alcohol are used as hydrogel raw materials, natural polymers are combined with synthetic polymers to construct composite polymer hydrogels.

3. The synthetic hydrogel according to claim 1, characterized in that The volume ratio of 5% to 8% of physcion@AgNPs and physcion methyl ether@AgNPs in colloidal state were added.

4. The synthetic hydrogel according to claim 1, characterized in that Green nanomaterials with synergistic antibacterial effects that have broad-spectrum antibacterial properties against Gram-positive and Gram-negative bacteria are added.

5. The synthetic hydrogel according to claim 1, characterized in that It can promote the formation of collagen, collagen fibers, and new blood vessels, improve the microenvironment of tissues around the wound, and thus accelerate the wound healing process.

6. The synthesized hydrogel described in claim 1 can be used to repair diabetic wounds, infected burn wounds, sensitive skin symptoms, etc.

7. A hydrogel for promoting collagen production, characterized in that it is prepared by the preparation method according to any one of claims 1 to 6.

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