Phycocyanin-containing hydrogel wound dressing as well as preparation method and application thereof

Abstract

The invention relates to a phycocyanin-containing hydrogel wound dressing as well as a preparation method and application thereof, phycocyanin, gelatin and hydrolyzed silk are taken as base materials, tetrakis hydroxymethyl phosphonium sulfate (THPS) is taken as a cross-linking agent, and the antibacterial healing-promoting hydrogel wound dressing is obtained through positive and negative charge attraction and Mannich reaction; wherein the mass percentage concentration ratio of phycocyanin to gelatin to hydrolyzed silk to THPS is 2%: 25%: 3%: 0.75%. A phosphate buffer solution (PBS) with the pH value of 7.4 is used as a solvent, phycocyanin, gelatin and hydrolyzed silk are used as raw materials, positive and negative charge attraction between phycocyanin and hydrolyzed silk and gelatin and THPS are used for crosslinking to form a stable network structure, and the hydrogel has good swelling performance, good self-healing effect and shape adaptability, can be used as a wound dressing, and can be used as a wound dressing for wound healing. The anti-oxidation capability, the antibacterial capability and the anti-inflammatory effect are realized.

Description

Technical Field

[0001] The present invention belongs to the field of biomaterials, and particularly relates to a phycocyanin-containing hydrogel wound dressing, a preparation method thereof, and an application thereof. Background Art

[0002] The skin is the largest organ of the human body and plays important physiological, chemical, and biological barrier roles. However, the skin is easily affected by the external environment, such as sunburn, bruising, and bumps, leading to problems such as bacterial infections. Traditional wound dressings only provide simple protection and are prone to exogenous bacterial infections. To overcome this problem, hydrogels have been widely used as a new type of wound dressing. Hydrogels have high oxygen permeability and excellent water swelling properties, and can prevent and control bacterial infections. Wound dressings can accelerate the healing process, but an ideal wound dressing should not only act as a physical barrier but also have antibacterial functions. Due to its hydrophilic polymer chains and three-dimensional polymer network structure, hydrogels have characteristics such as high expansibility, high water absorbency, and high water retention capacity, which provide excellent biocompatibility and the ability to control drug release for the hydrogels themselves. Both natural polymer hydrogels and synthetic polymer hydrogels have extensive applications, but traditional hydrogels are prone to rupture and have poor antibacterial effects. Protein-based hydrogels have become essential biomaterials due to their natural mildness and superior functional properties.

[0003] Phycocyanin is a fluorescent protein with strong water solubility and is also an edible natural blue pigment and the main protein of spirulina. In addition to being used as an edible pigment for desserts, beverages, etc., it also has important applications in the fields of health food, biomedicine, etc. Phycocyanin can induce the expression of HO-1 and has various anti-inflammatory activities. Its fluorescence characteristics and biological activities have made it widely used in the nutrition and pharmaceutical industries. Phycocyanin has a series of biological functions such as good antioxidant, anti-inflammatory, anti-tumor, immune enhancement, and anti-organ damage. It can inhibit the growth and reproduction of Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Streptococcus, etc., and has a broad antibacterial spectrum and strong antibacterial effect. However, phycocyanin has poor stability and is easily denatured and inactivated in a high-temperature environment, which restricts its application. Phycocyanin hydrogel can provide support as a wound healing matrix in damaged tissues, and its infrared and near-infrared characteristics can also provide additional assistance for wound healing detection, but its mechanical properties are relatively weak.

[0004] To improve the mechanical properties of phycocyanin hydrogel, gelatin and phycocyanin are used as the common matrix of the hydrogel. Gelatin is an easily obtainable material with good biocompatibility and biodegradability, and is commonly used in pharmaceutical and medical applications due to its properties in physiological environments. Gelatin contains the RGD sequence, which consists of arginine, glycine, and aspartic acid, and can enhance cell attachment. In addition, it has a viscous behavior in solution and can be physically cross-linked to prepare hydrogels as a bio-based material. It can form hydrogels with antibacterial agents such as iodine compounds, quaternary ammonium salts, and THPS.

[0005] Tetrakis(hydroxymethyl)phosphonium sulfate (THPS) is a colorless and transparent liquid, soluble in water and insoluble in acetone and ethanol. THPS is a by-product of low-cost cationic phosphorus salts for waste gas. As an antibacterial agent, it can effectively inhibit the growth of fungi, bacteria, and algae, and can be degraded into completely harmless substances after use; it can destroy the biofilm of bacteria and hardly produce bacterial drug resistance during the antibacterial process, and is a broad-spectrum, highly efficient, and environmentally friendly antibacterial agent.

[0006] Amine-rich phycocyanin, gelatin, and THPS form a hydrogel through the Mannich reaction. The Mannich reaction is a process in which the active hydrogen of formaldehyde is replaced by an amine to form a Mannich base or ammonium ion. The formed Mannich base or ammonium ion further reacts with THPS derivatives to generate amine coupling, thereby forming a hydrogel with phycocyanin and gelatin. Summary of the Invention

[0007] The purpose of the present invention is to provide a phycocyanin-containing hydrogel wound dressing and its preparation method and application to solve the problems raised in the above background technology. This hydrogel dressing not only provides a suitable environment for the growth of cells at the wound site, but also has the effects of antibacterial, controlling infection, and promoting wound healing.

[0008] To achieve the above technical objectives, the technical solution of the present invention provides a phycocyanin-containing hydrogel wound dressing and its preparation method:

[0009] Using phycocyanin, gelatin, and hydrolyzed silk as the base materials and tetrakis(hydroxymethyl)phosphonium sulfate (THPS) as the cross-linking agent, through positive and negative charge attraction and the Mannich reaction, an antibacterial and wound-healing promoting hydrogel wound dressing gel containing phycocyanin is obtained;

[0010] Among them, the mass percentage concentration ratio of phycocyanin, gelatin, hydrolyzed silk, and THPS is 2%:25%:3%:0.75%.

[0011] Furthermore, the preparation steps of the phycocyanin solution include:

[0012] S11. Add the purified phycocyanin powder to the PBS buffer solution with a pH value of 7.4, and shake and dissolve to obtain a phycocyanin solution.

[0013] Further, in step S11, the mass concentration of the phycocyanin solution is 0.1 g / mL. Further, the preparation steps of the gelatin solution include:

[0014] S21. Add gelatin particles to a PBS buffer solution with a pH value of 7.4, and dissolve them by heating and stirring in a water bath to obtain a gelatin solution.

[0015] Further, in step S21, the mass concentration of the gelatin solution is 0.4 g / mL, and the water bath temperature is 50 - 60 °C.

[0016] Further, the preparation steps of the hydrolyzed silk solution include:

[0017] S31. Add hydrolyzed silk powder to a PBS buffer solution with a pH value of 7.4, and dissolve it by shaking to obtain a hydrolyzed silk solution.

[0018] Further, in step S31, the mass concentration of the hydrolyzed silk solution is 0.3 g / mL.

[0019] Further, the cross-linking agent used is tetrakis(hydroxymethyl)phosphonium sulfate (THPS).

[0020] Further, the specific preparation steps include:

[0021] Dissolve the purified phycocyanin powder in a PBS buffer solution with a pH value of 7.4 to obtain solution A;

[0022] Dissolve gelatin particles in a PBS buffer solution with a pH value of 7.4 to obtain solution B;

[0023] Dissolve hydrolyzed silk powder in a PBS buffer solution with a pH value of 7.4 to obtain solution C;

[0024] Mix the PBS buffer solution with a pH value of 7.4, solution A, and solution C evenly at a volume ratio of 13:40:20 to obtain a hydrogel precursor solution;

[0025] Add solution B to the hydrogel precursor solution and stir to disperse it evenly at a volume ratio of 73:125;

[0026] Add 75% tetrakis(hydroxymethyl)phosphonium sulfate (THPS) to the evenly dispersed mixture and stir to mix it evenly at a volume ratio of 99:1;

[0027] Let it stand for 20 - 30 min to obtain a phycocyanin-containing hydrogel dressing.

[0028] The present invention provides an antibacterial and wound-healing-promoting hydrogel wound dressing gel containing phycocyanin.

[0029] The present invention provides an application of an antibacterial and wound-healing promoting hydrogel wound dressing containing phycocyanin in wound surface repair.

[0030] Compared with the prior art, the beneficial effects of the present invention include:

[0031] The present invention uses phycocyanin, gelatin and hydrolyzed silk as raw materials. Rich in amine phycocyanin, gelatin and THPS form a stable network structure through the Mannich reaction, and the adhesion performance of the glue is significantly improved by adding hydrolyzed silk; the antibacterial and wound-healing promoting hydrogel containing phycocyanin of the present invention also has good swelling performance; the hydrogel has good self-healing effect and shape adaptability and can be used as a wound dressing; since gelatin itself is a product after hydrolysis of natural collagen, the hydrogel prepared by the present invention has in vitro degradability; the phycocyanin used in the present invention can induce the expression of HO-1, has a variety of anti-inflammatory activities, and a series of biological functions such as good antioxidant and immunity enhancement of phycocyanin also endow the hydrogel with excellent anti-inflammatory, antioxidant and wound-healing promoting abilities. The preparation method of the present invention has low cost and is simple and easy to operate, and is expected to be industrialized. Description of the Drawings

[0032] Figure 1 It is a finished product diagram of the phycocyanin-containing hydrogel of the present invention.

[0033] Figure 2 It is a schematic diagram of the solidification state of the phycocyanin-containing hydrogel of the present invention at different standing times.

[0034] Figure 3 is a test diagram of the adhesion performance of the phycocyanin-containing hydrogel of the present invention.

[0035] Figure 4 It is a test diagram of the tensile performance of the phycocyanin-containing hydrogel of the present invention.

[0036] Figure 5 It is a self-healing effect diagram of the phycocyanin-containing hydrogel of the present invention.

[0037] Figure 6 is a bacteriostatic effect diagram of the hydrogel dressing of the phycocyanin-containing hydrogel example of the present invention, and the size of the bacteriostatic range is marked with an arrow. Among them, (A) is the inhibition of Escherichia coli (E. coli), and (B) is the inhibition effect diagram of Staphylococcus aureus (S. aureus).

[0038] Figure 7 is an antioxidant performance curve diagram of the phycocyanin-containing hydrogel of the present invention. Detailed Embodiments

[0039] In order to make the purpose, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0040] The present invention provides a phycocyanin-containing hydrogel wound dressing and a preparation method thereof. Using phycocyanin, gelatin and hydrolyzed silk as substrates and tetrakis(hydroxymethyl)phosphonium sulfate (THPS) as a crosslinking agent, an antibacterial and wound-healing promoting hydrogel wound dressing is obtained through positive and negative charge attraction and chemical crosslinking. At the same time, the added tetrakis(hydroxymethyl)phosphonium sulfate (THPS) can also enhance its antibacterial effect, and hydrolyzed silk enhances its adhesiveness, stretchability and self-healing performance. The mass concentration ratio of phycocyanin, gelatin, hydrolyzed silk and THPS is 2%:25%:3%:0.75%.

[0041] The main mechanism of the present invention: Traditional gelatin hydrogels basically have no antibacterial properties. The present invention makes the hydrogel have antibacterial, anti-inflammatory and antioxidant properties by adding phycocyanin. Secondly, the mechanical properties of the hydrogel are improved by adding hydrolyzed silk, so that the hydrogel has good adhesiveness, stretchability and self-healing properties. Finally, tetrakis(hydroxymethyl)phosphonium sulfate (THPS) is added, which can not only play a good crosslinking role to promote the formation of the hydrogel, but also prevent the generation of drug resistance and improve the antibacterial property of the hydrogel. In addition, the mechanical properties of the hydrogel are significantly improved.

[0042] The preparation method of the present invention specifically includes the following steps:

[0043] (1) Preparation of gelatin solution: Weigh gelatin and dissolve it in a phosphate buffer solution with pH = 7.4, and stir at 50-60 °C until completely dissolved. The concentration of the gelatin solution is 0.4 g / mL;

[0044] (2) Preparation of phycocyanin solution: Weigh purified phycocyanin powder and dissolve it in a phosphate buffer solution with pH = 7.4, and shake it until completely dissolved. The concentration of the phycocyanin solution is 0.1 g / mL.

[0045] (3) Preparation of hydrolyzed silk solution: Weigh hydrolyzed silk powder and dissolve it in a phosphate buffer solution with pH = 7.4, and shake it until completely dissolved. The concentration of the hydrolyzed silk solution is 0.3 g / mL.

[0046] (4) Label the purified phycocyanin solution to obtain solution A; label the gelatin solution to obtain solution B; label the hydrolyzed silk solution to obtain solution C;

[0047] (5) Under room temperature conditions, magnetically stir the PBS buffer solution with pH = 7.4, solution A and solution C in a volume ratio of 13:40:20 until they are evenly mixed to obtain a hydrogel precursor solution.

[0048] (6) Take the hydrogel precursor solution into a small glass bottle, add a certain amount of solution B and magnetically stir until it is evenly dispersed.

[0049] (7) Add 75% tetrakis (hydroxymethyl) phosphonium sulfate (THPS) to the uniformly dispersed mixture and stir to mix evenly, with a volume ratio of 99:1.

[0050] (8) Pour it into a mold and let it stand for 20 - 30 min to obtain the phycocyanin-containing hydrogel.

[0051] The phycocyanin-containing hydrogel of the present invention retains the characteristics of natural gelatin, and at the same time has good adhesiveness, stretchability, self-healing property, antioxidant property, immune-enhancing activity and good antibacterial property. The preparation method of the phycocyanin-containing hydrogel is simple and low-cost. Since gelatin is the product of hydrolysis of natural collagen, it has good biocompatibility and can be absorbed by the skin after degradation. The addition of phycocyanin and tetrakis (hydroxymethyl) phosphonium sulfate (THPS) endows the hydrogel with good free radical scavenging ability, destroys the growth of bacteria and thus plays an antibacterial role. The addition of hydrolyzed silk endows it with good mechanical properties.

[0052] Application of the phycocyanin-containing hydrogel obtained by the present invention in wound surface repair. The following further elaborates on the present invention through specific examples.

[0053] Example 1

[0054] An application of a phycocyanin-containing hydrogel is specifically prepared by the following method;

[0055] (1) Gelatin solution: Weigh 4 g of gelatin and dissolve it in 10 mL of phosphate buffer solution with pH = 7.4, and stir at 55 °C until completely dissolved. The concentration of the gelatin solution is 0.4 g / mL, and the mass-volume percentage (w / v) is 40%.

[0056] (2) Preparation of phycocyanin solution: Weigh 0.3 g of purified phycocyanin powder and dissolve it in 3 mL of phosphate buffer solution with pH = 7.4, and shake it until completely dissolved. The concentration of the phycocyanin solution is 0.1 g / mL, and the mass-volume percentage (w / v) is 10%.

[0057] (3) Preparation of hydrolyzed silk solution: Weigh 0.6 g of hydrolyzed silk powder and dissolve it in 2 mL of phosphate buffer solution with pH = 7.4, and shake it until completely dissolved. The concentration of the hydrolyzed silk solution is 0.3 g / Ml, and the mass-volume percentage (w / v) is 30%.

[0058] (4) Label the purified phycocyanin solution to obtain solution A; label the gelatin solution to obtain solution B; label the hydrolyzed silk solution to obtain solution C;

[0059] (5) Respectively take 0.13 mL of PBS buffer solution with a pH value of 7.4, 0.40 mL of solution A, and 0.20 mL of solution C into a vial, and perform magnetic stirring at room temperature. Mix evenly to obtain a hydrogel precursor solution.

[0060] (6) Add 1.25 mL of solution B to the small glass bottle of the hydrogel precursor solution and perform magnetic stirring to disperse evenly.

[0061] (7) Add 0.02 mL of 75% phosphonium hydroxymethyl sulfate (THPS) to the evenly dispersed mixture and stir to mix evenly.

[0062] (8) Pour it into a mold and let it stand for 20 - 30 min to obtain a phycocyanin-containing hydrogel.

[0063] Figure 1 This is the finished product diagram of the phycocyanin-containing hydrogel in this example.

[0064] Figure 2 It is a schematic diagram of the solidification state of the phycocyanin-containing hydrogel of the present invention used in this example at the standing times of 5 min, 15 min, and 25 min for verification.

[0065] Example 2

[0066] The difference from Example 1 is: change the raw material dosage in step (7), and other conditions are the same as those in Example 1.

[0067] Specifically:

[0068] (4) Record the purified phycocyanin solution to obtain solution A; record the gelatin solution to obtain solution B; record the hydrolyzed silk solution to obtain solution C;

[0069] (5) Respectively take 0.15 mL of PBS buffer solution with a pH value of 7.4, 0.40 mL of solution A, and 0.20 mL of solution C into a vial, and perform magnetic stirring at room temperature. Mix evenly to obtain a hydrogel precursor solution.

[0070] (7) Add 0.00 mL of 75% phosphonium hydroxymethyl sulfate (THPS) to the evenly dispersed mixture and stir to mix evenly.

[0071] Example 3

[0072] The difference from Example 1 is: change the raw material dosage in step (7), and other conditions are the same as those in Example 1.

[0073] Specifically:

[0074] (4) Record the purified phycocyanin solution to obtain solution A; record the gelatin solution to obtain solution B; record the hydrolyzed silk solution to obtain solution C;

[0075] (5) Respectively take 0.11 mL of PBS buffer solution with a pH value of 7.4, 0.40 mL of solution A, and 0.20 mL of solution C into a vial, and perform magnetic stirring at room temperature, and mix evenly to obtain a hydrogel precursor solution.

[0076] (7) Add 0.03 mL of 75% phosphonium sulfate tetrahydroxymethyl (THPS) to the uniformly dispersed mixture and stir to mix evenly.

[0077] Example 4

[0078] The difference from Example 1 is: change the raw material dosage in step (5), and other conditions are the same as those in Example 1.

[0079] Specifically:

[0080] (4) Record the purified phycocyanin solution to obtain solution A; the gelatin solution to obtain solution B; the hydrolyzed silk solution to obtain solution C;

[0081] (5) Respectively take 0.33 mL of PBS buffer solution with a pH value of 7.4, 0.40 mL of solution A, and 0.00 mL of solution C into a vial, and perform magnetic stirring at room temperature, and mix evenly to obtain a hydrogel precursor solution.

[0082] Example 5

[0083] The difference from Example 1 is: change the raw material dosage in step (5), and other conditions are the same as those in Example 1.

[0084] Specifically:

[0085] (4) Record the purified phycocyanin solution to obtain solution A; the gelatin solution to obtain solution B; the hydrolyzed silk solution to obtain solution C;

[0086] (5) Respectively take 0.03 mL of PBS buffer solution with a pH value of 7.4, 0.40 mL of solution A, and 0.3 mL of solution C into a vial, and perform magnetic stirring at room temperature, and mix evenly to obtain a hydrogel precursor solution.

[0087] Comparative Example 1

[0088] Compare Examples 1, 2, and 3 to draw a conclusion;

[0089] If the dosage of phosphonium sulfate tetrahydroxymethyl (THPS) is too much and the ratio with other substrates is inconsistent, the three-dimensional network structure of the prepared hydrogel is incomplete and the cross-linking density is large, resulting in poor mechanical strength and lack of elasticity of the hydrogel; if phosphonium sulfate tetrahydroxymethyl (THPS) is not added, the hydrogel is extremely easy to break and fragment. Therefore, precise control of the dosage and ratio of various raw materials is the key to the success of the experiment.

[0090] Comparative Example 2

[0091] Conclusions were drawn by comparing Examples 1, 4, and 5;

[0092] Adding hydrolyzed silk can effectively improve the adhesiveness and self-healing property of the hydrogel. However, if the amount of hydrolyzed silk added is too large, it will cause the hydrogel to be too sticky, adhere to the skin, and be difficult to shape.

[0093] Example 6

[0094] According to Examples 1-5 and Comparative Examples 1 and 2, the preparation scheme of Example 1 was obtained as the best preparation scheme. Therefore, the product prepared in Example 1 was used for the subsequent examples.

[0095] For the adhesion performance test of the phycocyanin-containing hydrogel, the specific test method is as follows:

[0096] The phycocyanin-containing hydrogel was attached to the surface of human skin and slowly peeled off. The test results are shown in Figure 3(A). No residue was left during peeling, and no irritation, damage, or pain was caused. The reason is that the hydrogel has good mechanical properties, indicating that the hydrogel has good stability. The phycocyanin-containing hydrogel was attached to the finger and the finger was bent repeatedly. The test results are shown in Figure 3(B). The hydrogel can tightly adhere to the bent finger and can be bent and stretched repeatedly without separating from the finger surface. This property enables the hydrogel as a wound dressing to adhere to a dynamic surface with excellent adhesion. By examining the compliance adhesion of the hydrogel to the finger and the peeling process of the adhered hydrogel, the hydrogel did not fall off significantly, forming a stable adhesion, and the peeling was painless and residue-free.

[0097] Example 7

[0098] The product prepared in Example 1 was used for this example.

[0099] For the tensile property test of the phycocyanin-containing hydrogel, the specific test method is as follows:

[0100] The prepared hydrogel was taken out from a rectangular mold with a length of 5 cm, and the two ends of the hydrogel were clamped with tweezers and stretched horizontally, and the measurements were recorded. The test results are as Figure 4 shown. The phycocyanin-containing hydrogel can be stretched to about twice its original length. Therefore, it shows that the hydrogel has good tensile properties.

[0101] Example 8

[0102] The product prepared in Example 1 was used for this example.

[0103] For the self-healing property test of the phycocyanin-containing hydrogel, the specific method is as follows:

[0104] Take out the prepared hydrogels from the rectangular and circular molds respectively. Cut the hydrogels into small rectangular and fan-shaped pieces. Join the two small hydrogel pieces together at the cut and let them stand for a few minutes. Hold both ends of the joined hydrogel with tweezers and stretch it horizontally. The test results are as Figure 5 shown. The two small hydrogel pieces are successfully adhered together and the adhesion effect is good. Therefore, it shows that the hydrogel has good self-healing performance.

[0105] Example 9

[0106] Use the product prepared in Example 1 for this example

[0107] Figure 6 is a scanning electron micrograph of a phycocyanin-containing hydrogel in this example. As can be seen from Figure 6, a large number of pore structures are distributed on the surface. It also shows that the gel can store a large amount of water and still maintain a certain stable shape. The pore wall structure is complete and has a thickness, and the pore wall is smooth and has strength. Therefore, the gel has good mechanical properties. Example 10

[0108] Use the product prepared in Example 1 for this example

[0109] Test the antibacterial performance of the phycocyanin-containing hydrogel. The specific method is as follows:

[0110] Drop 200 μL of the cultured Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) bacterial solutions onto two agar plates respectively, and spread the bacterial solutions evenly with a spreader. Take the hydrogel dressings prepared in Example 1 and Example 2, gently apply them on the agar plates coated with the bacterial solutions, and drop 0.75% tetrakis(hydroxymethyl)phosphonium sulfate (THPS) on the agar plates. Then transfer them to an incubator at 37 °C and culture for 24 h. Take out the agar plates from the incubator and observe the size of the inhibition zone. The results are as Figure 6A )(B) shown. It can be seen that the hydrogel dressing in Example 1 of the present invention has good antibacterial effects.

[0111] Example 12

[0112] Figure 7 is a graph showing the antioxidant performance of the phycocyanin-containing hydrogel of the present invention. Figure 7(A) shows the DPPH radical scavenging ability. As shown in Figure 7(A), as the phycocyanin concentration increases, the DPPH scavenging rate continuously increases, and the scavenging rate remains above 80%, and finally approaches 100%, proving that the phycocyanin has a high scavenging rate for DPPH and its antioxidant ability is very strong.

[0113] The specific advantages of the present invention are as follows:

[0114] 1. The present invention uses gelatin as a substrate, which has excellent biocompatibility. At the same time, phycocyanin is added, making the material have good anti-inflammatory, antioxidant and wound-healing promoting abilities.

[0115] 2. The present invention uses tetrakis(hydroxymethyl)phosphonium sulfate (THPS) as a crosslinking agent, which can form a stable network structure and also provide antibacterial properties.

[0116] 3. The preparation of the phycocyanin-containing hydrogel in the present invention can play roles in antibacterial, antioxidant, anti-inflammatory and wound-healing promotion. It also has good swelling properties, self-healing effect and shape adaptability.

[0117] 4. The preparation method of the present invention has low cost, is simple and easy to operate, and is expected to achieve industrialization.

[0118] The specific embodiments of the present invention described above do not constitute a limitation to the protection scope of the present invention. Any other corresponding changes and deformations made based on the technical concept of the present invention shall be included in the protection scope of the claims of the present invention.

Claims

1. A preparation method of a phycocyanin hydrogel wound dressing, characterized in that, it includes the following steps: Using phycocyanin, gelatin and hydrolyzed silk as substrates, and tetrakis(hydroxymethyl)phosphonium sulfate (THPS) as a crosslinking agent, an antibacterial and wound-healing promoting hydrogel wound dressing gel containing phycocyanin is obtained through positive and negative charge attraction and Mannich reaction; wherein, the mass percentage concentration ratio of phycocyanin, gelatin, hydrolyzed silk and THPS is 2%:25%:3%:0.75%.

2. The preparation method of a phycocyanin hydrogel wound dressing according to claim 1, characterized in that, the preparation steps of the phycocyanin solution include: S11. Add the purified phycocyanin powder into the PBS buffer solution with a pH value of 7.4, shake and dissolve to obtain a phycocyanin solution.

3. The preparation method of a phycocyanin hydrogel wound dressing according to claim 2, characterized in that, in step S11, the mass concentration of the phycocyanin solution is 0.1 g / mL.

4. The preparation method of a phycocyanin hydrogel wound dressing according to claim 1, characterized in that, the preparation steps of the gelatin solution include: S21. Add gelatin particles into the PBS buffer solution with a pH value of 7.4, heat and stir in a water bath to dissolve to obtain a gelatin solution.

5. The preparation method of a phycocyanin hydrogel wound dressing according to claim 4, characterized in that, in step S21, the mass concentration of the gelatin solution is 0.4 g / mL, and the water bath temperature is 50 - 60 °C.

6. The preparation method of a phycocyanin hydrogel wound dressing according to claim 1, characterized in that, the preparation steps of the hydrolyzed silk solution include: S31. Add the hydrolyzed silk powder into the PBS buffer solution with a pH value of 7.4, shake and dissolve to obtain a hydrolyzed silk solution.

7. The preparation method of a phycocyanin hydrogel wound dressing according to claim 6, characterized in that, in step S31, the mass concentration of the hydrolyzed silk solution is 0.3 g / mL.

8. The preparation method of a phycocyanin hydrogel wound dressing according to claim 1, characterized in that, the crosslinking agent used is tetrakis(hydroxymethyl)phosphonium sulfate (THPS).

9. The preparation method of a phycocyanin hydrogel wound dressing according to claim 1, characterized in that, the specific preparation steps include: Take the purified phycocyanin powder and dissolve it in the PBS buffer solution with a pH value of 7.4 to obtain solution A; Take gelatin particles and dissolve them in the PBS buffer solution with a pH value of 7.4 to obtain solution B; Take the hydrolyzed silk powder and dissolve it in the PBS buffer solution with a pH value of 7.4 to obtain solution C; Mix the PBS buffer solution with a pH value of 7.4, solution A and solution C evenly at a volume ratio of 13:40:20 to obtain a hydrogel precursor solution; Add solution B to the hydrogel precursor solution and stir to disperse evenly, with a volume ratio of 73:125; Add 75% of tetrakis(hydroxymethyl)phosphonium sulfate (THPS) to the uniformly dispersed mixture and stir to mix evenly, with a volume ratio of 99:1; Let it stand for 20 - 30 min to obtain the phycocyanin-containing hydrogel dressing.

10. The phycocyanin-containing hydrogel dressing prepared by the preparation method according to any one of claims 1 - 9.

11. Use of a phycocyanin-containing hydrogel wound dressing according to claim 10 in wound surface repair.

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