Polluted water sampling device and operation process

By designing a contaminated water body sampling device including a sample box, storage block, heating plate and sealed film, the problems of water flow agitation and sample leakage during the sampling process in the prior art are solved, and more accurate water body detection and laboratory safety protection are achieved.

CN120141927AInactive Publication Date: 2025-06-13CHANGDE ZHISEN ENVIRONMENTAL PROTECTION TECHNOLOGY CO LTD
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Patent Information

Application Number
CN202510485156.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-04-17
Publication Date
2025-06-13
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

The existing contaminated water sampling device will agitate the water flow when inserted into the water, causing microorganisms of different water depths to be agitated to other height levels, affecting the accuracy of the detection data. At the same time, the sampling volume is difficult to control, which can easily lead to water leakage, which may carry toxic substances, affecting laboratory safety.

Method used

A sampling device including a sample box, a storage block, a heating plate, a sampling mechanism and a sealed film are designed. Through the design of the storage block and the cross plate, the sample chamber can be separated into multiple isolation chambers to avoid intermingling of microorganisms at the depth of the water. The sampling mechanism achieves accurate sampling and sealing of the sample through the cooperation of the glass test tube and the syringe to avoid leakage of water.

Benefits of technology

This device effectively avoids the intermixing of microorganisms in deep water, improves the accuracy of detection data, and avoids leakage of water through closed sampling, protects the safety of the laboratory.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention is applicable to the field of ecological detection, and provides a sampling device for polluted water and an operation process, the sampling device comprises a sample box, the sample box is divided into three chambers by a storage block, the left chamber and the right chamber form a sample chamber, the middle chamber forms a positioning chamber, and heating plates are arranged on the left side wall and the right side wall of the sample box; the sampling mechanism is arranged on the front side wall of the sample box; an expansion assembly matched with the cross plate is arranged in the positioning cavity. When the device is used, a worker manually and slowly presses a positioning plate, in the downward pushing process of the positioning plate and an arc-shaped slope surface, the arc-shaped slope surface is pressed on an extrusion rod to push a cross plate towards the outer side, the cross plate can transversely penetrate through the interior of a penetrating groove, and the cross plate can abut against a sealing film to penetrate through a gap of a steel bar; an injector is inserted into the air inlet nozzle opposite to the liquid inlet nozzle, and the injector can inject air according to milliliters of liquid taken out of the glass test tube. Therefore, the function of accurate sampling is realized.
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Description

Technical Field

[0001] The present invention belongs to the field of ecological detection, and particularly relates to a sampling device and operation process for polluted water bodies. Background Art

[0002] With the development of industrial technology, modern ecological detection technology has become increasingly perfect. The rivers and waters near chemical plants will be polluted. These chemical materials enter the water body and will affect the microorganisms inside. The impact of chemical waste on aquatic organisms is unpredictable. This requires sampling and inspection of the water in the water area, which requires a sampling device for the water area. However, at present, a series of problems that need to be solved urgently have emerged in the actual application of the current sampling device. First of all, when the existing sampling equipment is inserted into the water, it will stir the water flow. Although this can extract the water sample intact, the living environment of microorganisms in the water body is at different water depths. During the process of inserting the sampling device into the water, the water at different heights will be convected, and the microorganisms at different water depths will be stirred to other height levels, resulting in inaccurate detection data of the water depths where various microorganisms live. Secondly, it is difficult to control the amount of water sampled, and there is also a risk of water splashing out during the sampling process. These water samples are likely to be contaminated by toxic substances from chemical plants, which will have an adverse impact on the laboratory. Summary of the Invention

[0003] The purpose of the present invention is to provide a sampling device and operation process for polluted water bodies in view of the deficiencies of the prior art, so as to solve the problems mentioned in the background art.

[0004] To solve the above problems, the present invention provides the following technical solutions: A sampling device for polluted water bodies includes a sample box, two storage blocks arranged inside the sample box. The storage blocks separate the sample box into three chambers. The left and right chambers form sample chambers, and the middle chamber forms a positioning chamber. Heating plates are arranged on the left and right side walls of the sample box; a sampling mechanism arranged on the front side wall of the sample box; a winding roller is rotatably arranged at the top of the storage block, a sealed film is pulled out from the outside of the winding roller, the bottom edge of the sealed film is fixedly connected by hot melting to the bottom of the sample chamber, and an elastic winding component is arranged at the end of the winding roller; three insertion slots are arranged inside the storage block, and the three insertion slots are evenly arranged up and down. A cross plate is inserted into the insertion slots. An expansion component matching the cross plate is arranged inside the positioning chamber; two steel bars are arranged at the port of the insertion slot, and the gap between the two steel bars is equal to the width of the insertion slot. The cross plate passes through the gap between the steel bars and is attached with the sealed film. Three groups of rubber strips are arranged on the side walls of the sample chamber, and the edge of the sealed film fits the rubber strips. The hardness of the material of the sealed film is greater than that of the rubber strips.

[0005] Preferably, the expansion component includes vertical guide rails on the front and rear side walls of the positioning chamber. A pressing slider is slidably sleeved on the vertical guide rails. A positioning plate is arranged in the middle of the pressing slider. Connecting bridges connected to the pressing slider are arranged on the front and rear side walls of the positioning plate. A lifting handle is arranged at the top end of the positioning plate. An arc-shaped slope is arranged at the bottom end of the positioning plate. A resisting rod attached to the arc-shaped slope is arranged at the inner end of the cross plate.

[0006] Preferably, the resisting rod includes an expansion block on the side wall of the cross plate. An extrusion rod is arranged at the end of the expansion block. The extrusion rod is attached to the side wall of the arc-shaped slope.

[0007] Preferably, the extrusion rod is made of stainless steel. The extrusion rod is polished. Machine oil is applied to the extrusion rod and the arc-shaped slope.

[0008] Preferably, the elastic winding component includes two shaft seats at the top end of the storage block. Shaft rods inserted into the shaft seats are arranged at both ends of the winding roller. An extension torsion rod is arranged on the front shaft rod. A resilient flap is arranged at the end of the extension torsion rod. A torsion spring is sleeved outside the extension torsion rod. One end of the torsion spring is connected to the resilient flap, and the other end of the torsion spring is connected to the side wall of the shaft seat.

[0009] Preferably, the sampling mechanism includes an annular frame. The annular frame is connected to the side wall of the sample box through a clamping component. Three air inlets are arranged on the front side wall of the sample box. Three liquid outlets are arranged on the rear side wall of the sample box. Eight placement holes are arranged on the annular frame. Glass test tubes are installed inside the placement holes. A clamping plug is detachably installed at the top opening of the glass test tube. A cannula is arranged in the middle of the clamping plug. Air holes are also arranged on the clamping plug. An optometry component is arranged in the middle of the annular frame.

[0010] Preferably, the clamping component includes a connecting plate on the side wall of the annular frame. A magnet sheet is arranged at the end of the connecting plate. A rectangular pit matching the cross section of the connecting plate is arranged on the side wall of the sample box. Iron sheets are arranged inside the rectangular pit.

[0011] Preferably, the optometry component includes an extension plate at the bottom end of the sample box. An incandescent lamp is arranged at the end of the extension plate. The incandescent lamp is inserted into the inside of the annular frame.

[0012] Preferably, it includes the following steps: S1. Extract samples from the river water near the chemical plant. The sample liquid is put into the inside of the sample chamber. The sample liquid in the two sample chambers is heated by a heating plate. The sample liquid in the left and right sample chambers is heated to 20 degrees Celsius, and the sample liquid in the right sample chamber is heated to 35 degrees Celsius. The liquid in the sample chamber is left still for 90 minutes to allow the substances in the aqueous solution to layer. S2. The staff manually and slowly press the positioning plate. During the process of the positioning plate and the arc-shaped slope advancing downward, the arc-shaped slope presses on the extrusion rod to push the cross plate outward. The cross plate will horizontally penetrate inside the insertion slot, and the cross plate will push against the airtight film to pass through the gaps between the steel bars. Then, the airtight film begins to be released from the winding roller to form three layers. When the three layers of airtight film touch the other edge of the sample chamber, the sample chamber can be separated into four upper and lower isolation chambers; S3. Take out a glass test tube and insert the cannula at the port of the glass test tube into the liquid outlet nozzle. Then, use a syringe to insert it into the air inlet nozzle opposite the liquid outlet nozzle. For the amount of liquid to be taken out from the glass test tube, the same amount of air is injected by the syringe. After sampling eight glass test tubes, turn on the incandescent lamp. The staff record the light transmittance of the solutions inside the eight glass test tubes, and then send the glass test tubes to the microscope equipment to observe microorganisms.

[0013] Compared with the prior art, the embodiments of the present application mainly have the following beneficial effects: When the device is in use, the staff manually and slowly press the positioning plate. During the process of the positioning plate and the arc-shaped slope advancing downward, the arc-shaped slope presses on the extrusion rod to push the cross plate outward. The cross plate will horizontally penetrate inside the insertion slot, and the cross plate will push against the airtight film to pass through the gaps between the steel bars. Then, the airtight film begins to be released from the winding roller to form three layers. When the three layers of airtight film touch the other edge of the sample chamber, the sample chamber can be separated into four upper and lower isolation chambers, which ensures that the liquids at different water depths will not be mixed during the sampling process, effectively improving the data accuracy of water body detection. Take out a glass test tube and insert the cannula at the port of the glass test tube into the liquid outlet nozzle. Then, use a syringe to insert it into the air inlet nozzle opposite the liquid outlet nozzle. For the amount of liquid to be taken out from the glass test tube, the same amount of air is injected by the syringe, avoiding the spillage of the sampled liquid and effectively protecting the experimental site and the personal safety of the operators. Description of the Drawings

[0014] Figure 1 It is a front view schematic diagram of the present invention.

[0015] Figure 2 It is a side view schematic diagram of the present invention.

[0016] Figure 3 It is a sectional view schematic diagram of the present invention.

[0017] Figure 4 It is a schematic diagram of the cross plate of the present invention.

[0018] Figure 5 It is a schematic diagram of the present invention.

[0019] Figure 6 Schematic diagram of the present invention.

[0020] Figure 7 Schematic diagram of the present invention.

[0021] Figure 8 Schematic diagram of the present invention.

[0022] Explanation of reference numerals in the drawings: Sample box 1, air inlet nozzle 101, heating plate 102, liquid outlet nozzle 103, positioning chamber 104, sample chamber 105, storage block 106, rubber strip 107, annular frame 2, connecting plate 201, glass test tube 202, engaging plug 203, insertion tube 204, air hole 205, vertical guide rail 3, positioning plate 4, connecting bridge 401, lifting handle 402, arc-shaped slope 403, shaft seat 5, winding roller 501, airtight film 502, extending torsion bar 503, elastic flap 504, torsion spring 505, steel bar 506, insertion slot 6, cross plate 601, expansion block 602, extrusion rod 603, extension plate 7, incandescent lamp 701. Detailed implementation manners

[0023] To make the objectives, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below in conjunction with specific embodiments of the present invention and the corresponding drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0024] The present invention provides a sampling device for polluted water bodies, as Figure 1-8As shown in the figure, it includes a sample box 1; two storage blocks 106 are arranged inside the sample box 1, and the storage blocks 106 separate the sample box 1 into three chambers. The left and right chambers form a sample chamber 105, and the middle chamber forms a positioning chamber 104. Heating plates 102 are arranged on the left and right side walls of the sample box 1; a sampling mechanism is arranged on the front side wall of the sample box 1; a winding roller 501 is rotatably arranged at the top of the storage block 106, a sealed film 502 is drawn out from the outside of the winding roller 501, and the bottom edge of the sealed film 502 is fixedly connected by hot melting to the bottom of the sample chamber 105. An elastic winding assembly is arranged at the end of the winding roller 501; three insertion slots 6 are arranged inside the storage block 106, and the three insertion slots 6 are evenly arranged up and down. A cross plate 601 is inserted into the insertion slot 6, and an expansion assembly matching the cross plate 601 is arranged inside the positioning chamber 104; two steel bars 506 are arranged at the port of the insertion slot 6, and the gap between the two steel bars 506 is equal to the width of the insertion slot 6. The cross plate 601 passes through the gap between the steel bars 506 and brings the sealed film 502 along. Three groups of rubber strips 107 are arranged on the side wall of the sample chamber 105, and the edge of the sealed film 502 fits the rubber strips 107. The hardness of the material of the sealed film 502 is greater than the hardness of the rubber strips 107.

[0025] In this embodiment, samples are extracted from the river water near a chemical plant, and the sample liquid is put into the sample chamber 105. The heating plates 102 are used to heat the sample liquid in the two sample chambers 105. The sample liquid in the left and right sample chambers 105 is heated to 20 degrees Celsius, and the sample liquid in the right sample chamber 105 is heated to 35 degrees Celsius. The liquid in the sample chamber 105 is allowed to stand for 90 minutes to stratify the substances in the aqueous solution. The cross plate 601 will horizontally penetrate inside the insertion slot 6, and the cross plate 601 will push against the sealed film 502 and pass through the gap between the steel bars 506. Then, the sealed film 502 starts to be released from the winding roller 501 to form three layers. When the three layers of the sealed film 502 touch the other edge of the sample chamber 105, the sample chamber 105 can be separated into four upper and lower isolation chambers. Eight isolation cavities for sample liquid are formed inside the sample box 1. When sampling the sample liquid in each isolation cavity, no convection will occur. In this way, the staff can obtain microbial samples at different water depths, so as to accurately master the enrichment of microorganisms at different water depths.

[0026] In a further embodiment of the present invention, as Figure 1-4As shown, the expansion component includes the vertical guide rails 3 on the front and rear side walls of the positioning chamber 104. A pressing slider is slidably sleeved on the vertical guide rails 3. A positioning plate 4 is arranged in the middle of the pressing slider. Connecting bridges 401 connected to the pressing slider are arranged on the front and rear side walls of the positioning plate 4. A lifting handle 402 is arranged at the top of the positioning plate 4. An arc-shaped slope 403 is arranged at the bottom of the positioning plate 4. A resisting rod that fits on the arc-shaped slope 403 is arranged at the inner end of the cross plate 601.

[0027] In this embodiment, the staff manually and slowly presses the positioning plate 4. During the process of the positioning plate 4 and the arc-shaped slope 403 advancing downward, the arc-shaped slope 403 presses the cross plate 601 to advance outward. The cross plate 601 will horizontally penetrate inside the insertion slot 6, which is convenient for stably advancing the cross plate 601, and there will be no large agitation of the liquid during the horizontal insertion of the cross plate 601.

[0028] In a further embodiment of the present invention, as Figure 1-4 shown, the resisting rod includes the expansion block 602 on the side wall of the cross plate 601. An extrusion rod 603 is arranged at the end of the expansion block 602. The extrusion rod 603 fits on the side wall of the arc-shaped slope 403.

[0029] In this embodiment, the arc-shaped slope 403 presses on the extrusion rod 603 to push the cross plate 601 outward. After the expansion block 602 and the extrusion rod 603 are used a certain number of times, they are disassembled for maintenance, so as to keep the friction between the arc-shaped slope 403 and the extrusion rod 603 more lubricated.

[0030] In a further embodiment of the present invention, as Figure 1-3 shown, the extrusion rod 603 is made of stainless steel. The extrusion rod 603 has been polished. Machine oil is applied on the extrusion rod 603 and the arc-shaped slope 403.

[0031] In this embodiment, the machine oil between the extrusion rod 603 and the arc-shaped slope 403 reduces the friction force and increases the corrosion resistance of the stainless steel extrusion rod 603.

[0032] In a further embodiment of the present invention, as Figure 1-4 shown, the elastic winding component includes two shaft seats 5 at the top of the storage block 106. Shaft rods inserted into the shaft seats 5 are arranged at both ends of the winding roller 501. An extension torsion rod 503 is arranged on the front shaft rod. A resilient flap 504 is arranged at the end of the extension torsion rod 503. A torsion spring 505 is sleeved outside the extension torsion rod 503. One end of the torsion spring 505 is connected to the resilient flap 504, and the other end of the torsion spring 505 is connected to the side wall of the shaft seat 5.

[0033] In this embodiment, the sealed film 502 starts to be released from the winding roller 501 to form three layers. When the winding roller 501 releases the sealed film 502, potential energy is input into the torsion spring. When the sealed film 502 needs to be wound up, the positioning plate 4 is lifted, and the potential energy stored in the torsion spring 505 is converted into the winding kinetic energy of the winding roller 501. Furthermore, the sample chamber 105 will be completely opened to tilt the sample, and the sample chamber 105 is injected with samples again for the next inspection operation.

[0034] In a further embodiment of the present invention, as Figure 1-4 shown, the sampling mechanism includes an annular frame 2. The annular frame 2 is connected to the side wall of the sample box 1 through a clamping component. Three air inlet nozzles 101 are provided on the front side wall of the sample box 1, and three liquid outlet nozzles 103 are provided on the rear side wall of the sample box 1. Eight placement holes are provided on the annular frame 2, and glass test tubes 202 are installed inside the placement holes. A clamping plug 203 is detachably installed at the top opening of the glass test tube 202. A cannula 204 is provided in the middle of the clamping plug 203, and an air hole 205 is also provided on the clamping plug 203. An optometry component is provided in the middle of the annular frame 2.

[0035] In this embodiment, a glass test tube 202 is taken out and the cannula 204 at the port of the glass test tube 202 is inserted into the liquid outlet nozzle 103. Then, a syringe is inserted into the air inlet nozzle 101 opposite to the liquid outlet nozzle 103. For every milliliter of liquid to be taken out from the glass test tube 202, the same amount of air is injected by the syringe. Then, the glass test tube 202 is sent to a microscope device to observe microorganisms. Compared with the existing sampling methods, the previous sampling methods are likely to cause the liquid to spill out. If the aqueous sample contains toxic substances, it will have an adverse impact on the laboratory.

[0036] In a further embodiment of the present invention, as Figure 7-8 shown, the clamping component includes a connecting plate 201 on the side wall of the annular frame 2. A magnet sheet is provided at the end of the connecting plate 201. A rectangular pit matching the cross-section of the connecting plate 201 is provided on the side wall of the sample box 1, and iron sheets are provided inside the rectangular pit.

[0037] In this embodiment, when the annular frame 2 and the connecting plate 201 are inserted into the rectangular pit, the iron sheet will be adsorbed by the magnet sheet. After each sampling completed by the annular frame 2 is replaced, it only needs to be pulled out and replaced. Therefore, the replacement operation of the annular frame 2 is very fast.

[0038] In a further embodiment of the present invention, as Figure 5-6 shown, the optometry component includes an extension plate 7 at the bottom end of the sample box 1. An incandescent lamp 701 is provided at the end of the extension plate 7, and the incandescent lamp 701 is inserted inside the annular frame 2.

[0039] In this embodiment, after the sampling of the eight glass test tubes 202 is completed, the incandescent lamp 701 is lit, and the staff records the light transmittance of the solution inside the eight glass test tubes 202.

[0040] It includes the following steps: S1. Extract samples from the river water near the chemical plant, put the sample liquid into the interior of the sample chamber 105, and use the heating plate 102 to heat the sample liquid in the two sample chambers 105. The sample liquid in the left and right sample chambers 105 is heated to 20 degrees Celsius, and the sample liquid in the right sample chamber 105 is heated to 35 degrees Celsius. The liquid in the sample chamber 105 is left stationary for 90 minutes to allow the substances in the aqueous solution to stratify. S2. The staff manually and slowly presses the positioning plate 4. During the process of the positioning plate 4 and the arc-shaped slope 403 advancing downward, the arc-shaped slope 403 presses on the extrusion rod 603 to push the cross plate 601 outward. The cross plate 601 will horizontally penetrate inside the insertion slot 6. The cross plate 601 will push against the airtight film 502 and pass through the gap of the steel bar 506. Then, the airtight film 502 starts to be released from the winding roller 501 to form three layers. When the three layers of the airtight film 502 touch the other edge of the sample chamber 105, the sample chamber 105 can be separated into four upper and lower isolation chambers. S3. Take out a glass test tube 202 and insert the cannula 204 at the port of the glass test tube 202 into the liquid outlet nozzle 103. Then, use a syringe to insert it into the air inlet nozzle 101 opposite to the liquid outlet nozzle 103. The amount of air injected by the syringe is the same as the amount of liquid to be taken out from the glass test tube 202. After the sampling of the eight glass test tubes 202 is completed, the incandescent lamp 701 is lit, and the staff records the light transmittance of the solution inside the eight glass test tubes 202. Then, the glass test tubes 202 are sent to the microscope equipment to observe microorganisms.

[0041] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the protection scope of the invention. Obviously, the described embodiments are only some embodiments of the present invention, rather than all embodiments. Based on these embodiments, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention. Although the present invention has been described in detail with reference to the above embodiments, those of ordinary skill in the art can still, without conflict, make combinations, additions, deletions, or other adjustments to the features in the embodiments of the present invention according to the situation without creative efforts, so as to obtain different technical solutions that essentially do not deviate from the concept of the present invention. These technical solutions also belong to the scope of protection of the present invention.

Claims

1. A sampling device for polluted water, characterized in that: Includes sample box (1); Two storage blocks (106) are arranged inside the sample box (1), the storage blocks (106) separate the sample box (1) into three chambers, the left and right chambers form sample chambers (105), and the middle chamber forms a positioning chamber (104), and heating plates (102) are arranged on the left and right side walls of the sample box (1); A sampling mechanism arranged on the front side wall of the sample box (1); The top of the storage block (106) is rotatably provided with a winding roller (501), the outer side of the winding roller (501) pulls out a sealed film (502), the bottom edge of the sealed film (502) is hot-melt fixedly connected to the bottom of the sample chamber (105), and the end of the winding roller (501) is provided with an elastic winding component; The storage block (106) is provided with three through slots (6) in the interior, the three through slots (6) are evenly arranged up and down, a cross plate (601) is inserted in the through slots (6), and an expansion component matching the cross plate (601) is provided in the interior of the positioning chamber (104); Two steel bars (506) are arranged at the end of the through-slot (6), the gap between the two steel bars (506) is equal to the width of the through-slot (6), the cross plate (601) passes through the gap between the steel bars (506) and is attached with a sealed film (502), three groups of rubber strips (107) are arranged on the side wall of the sample chamber (105), the edge of the sealed film (502) is attached to the rubber strip (107), and the material hardness of the sealed film (502) is greater than the hardness of the rubber strip (107).

2. A polluted water sampling device according to claim 1, characterized in that: The expansion assembly comprises a vertical guide rail (3) on the front and rear side walls of the positioning chamber (104), a pressing slider is slidably mounted on the vertical guide rail (3), a positioning plate (4) is arranged in the middle of the pressing slider, a connecting bridge (401) connected to the pressing slider is arranged on the front and rear side walls of the positioning plate (4), a lifting handle (402) is arranged at the top end of the positioning plate (4), an arc-shaped slope (403) is arranged at the bottom end of the positioning plate (4), and a resistance rod that fits on the arc-shaped slope (403) is arranged at the inner end of the cross plate (601).

3. A polluted water sampling device according to claim 2, characterized in that: The abutment rod comprises an expansion block (602) on the side wall of the cross plate (601), and an extrusion rod (603) is provided at the end of the expansion block (602), and the extrusion rod (603) is attached to the side wall of the arc-shaped slope surface (403).

4. A polluted water sampling device according to claim 3, characterized in that: The extrusion rod (603) is made of stainless steel, is ground and polished, and is coated with organic oil on the extrusion rod (603) and the curved slope (403).

5. The sampling device for polluted water according to claim 1, characterized in that: The elastic winding assembly comprises two shaft seats (5) at the top of the storage block (106); shaft rods inserted into the shaft seats (5) are arranged at both ends of the winding roller (501); an extension torsion bar (503) is arranged on the front end of the shaft rod; an elastic flap (504) is arranged at the end of the extension torsion bar (503); a torsion spring (505) is sleeved on the outer side of the extension torsion bar (503); one end of the torsion spring (505) is connected to the elastic flap (504); and the other end of the torsion spring (505) is connected to the side wall of the shaft seat (5).

6. A polluted water sampling device according to claim 1, characterized in that: The sampling mechanism comprises an annular frame (2), the annular frame (2) being connected to the side wall of the sample box (1) via a snap-fit ​​assembly, three groups of air inlet nozzles (101) being arranged on the front side wall of the sample box (1), three groups of liquid outlet nozzles (103) being arranged on the rear side wall of the sample box (1), eight placement holes being arranged on the annular frame (2), glass test tubes (202) being installed inside the placement holes, a snap-fit ​​plug (203) being detachably installed at the top opening of the glass test tube (202), a cannula (204) being arranged in the middle of the snap-fit ​​plug (203), an air hole (205) being also arranged on the snap-fit ​​plug (203), and an optometry assembly being arranged in the middle of the annular frame (2).

7. A polluted water sampling device according to claim 6, characterized in that: The clamping assembly comprises a connecting plate (201) on the side wall of the annular frame (2), a magnet sheet being arranged at the end of the connecting plate (201), and a rectangular pit matching the cross section of the connecting plate (201) being arranged on the side wall of the sample box (1), and an iron sheet being arranged inside the rectangular pit.

8. A polluted water sampling device according to claim 6, characterized in that: The optometry assembly comprises an extension plate (7) at the bottom end of the sample box (1), an incandescent lamp (701) is arranged at the end of the extension plate (7), and the incandescent lamp (701) is inserted into the interior of the annular frame (2).

9. The operating process of the sampling device for polluted water according to any one of claims 1 to 8, characterized in that: The following steps are involved: S1. Extract samples from river water near a chemical plant, put the sample water into the sample chamber (105), use the heating plate (102) to heat the sample water in the two sample chambers (105), heat the water in the left and right sample chambers (105) to 20 degrees Celsius, and heat the water in the right sample chamber (105) to 35 degrees Celsius. Let the water in the sample chamber (105) stand for 90 minutes to allow the substances in the aqueous solution to separate into layers; S2. The staff manually and slowly presses the positioning plate (4). When the positioning plate (4) and the arc-shaped slope (403) are pushed downward, the arc-shaped slope (403) is pressed against the extrusion rod (603) to push the cross plate (601) outward. The cross plate (601) will be inserted horizontally inside the through slot (6). The cross plate (601) will support the sealed film (502) to pass through the gap between the steel bars (506). Then, the sealed film (502) begins to be released from the winding roller (501) to form three layers. When the three layers of sealed films (502) touch the other edge of the sample chamber (105), the sample chamber (105) can be separated into four upper and lower isolation chambers. S3. Take out a glass test tube (202) and insert the cannula (204) at the end of the glass test tube (202) into the liquid outlet (103), and then use a syringe to insert it into the air inlet (101) opposite to the liquid outlet (103). The syringe will inject the same amount of air as the amount of liquid to be taken out of the glass test tube (202). After the eight glass test tubes (202) are sampled, the incandescent lamp (701) will be lit. The staff will record the light transmittance of the solution inside the eight glass test tubes (202), and then send the glass test tubes (202) to the microscope equipment to observe the microorganisms.