Experimental device and experimental method for researching effect of aerogenic bacteria on plant growth

Abstract

The invention discloses an experimental device and an experimental method for studying the effect of aerogenic bacteria on plant growth, the experimental device comprises an experimental cabin and a control system, the experimental cabin is divided into an upper-layer plant culture area and a lower-layer microorganism culture area by a horizontally arranged partition plate, a planting substrate is placed at the top end of the partition plate, and a plurality of air holes are formed in the partition plate; an environment simulation regulation and control system is arranged in the plant culture area, a sterilization system and an environment regulation and control system are arranged in the microorganism culture area, and the environment simulation regulation and control system, the sterilization system and the environment regulation and control system are all electrically connected with the control system. According to the technical scheme, a microorganism culture part and a plant planting part are integrated, gas generated by aerogenic bacteria can directly act on plants, an intermediate collection link is not needed, experimental steps are simplified, errors generated in the transfer process are avoided, and the accuracy of experimental research is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial and plant physiology research, and more specifically, to an experimental device and method for studying the effect of gas-producing bacteria on plant growth. Background Art

[0002] In the field of plant biology research, exploring the interaction mechanism between microorganisms and plants, especially the impact of gas-producing bacteria on plant growth, has become a research hotspot in recent years. Existing technical solutions mainly rely on traditional laboratory culture dishes and gas collection devices to study the activities of gas-producing bacteria and their effects on plant growth. These solutions generally involve inoculating gas-producing bacteria near the plant roots in a closed system and evaluating the activity of gas-producing bacteria and their potential promoting effects on plant growth by regularly measuring changes in gas components (such as carbon dioxide, oxygen, etc.).

[0003] However, the existing technical solutions have significant drawbacks: Firstly, it is difficult to precisely control environmental conditions (such as temperature, humidity, light, etc.) in the traditional culture dish system, which limits the accuracy and repeatability of the experiment; Secondly, the gas collection and analysis process is cumbersome and time-consuming, which is not conducive to efficiently conducting large-scale experiments; Moreover, due to the need for gas collection and transfer in the existing device, the research error is increased, making the experimental results inaccurate and difficult to deeply explore its mechanism of action. Therefore, it is particularly important to develop a device that can overcome the above defects and achieve more precise, efficient, and in-depth research. Summary of the Invention

[0004] In view of this, the present invention provides an integrated research device and method, which combines the bacterial culture module and the plant culture module to improve the accuracy of research results and simplify the research process.

[0005] To achieve the above object, the present invention adopts the following technical solutions:

[0006] An experimental device for studying the effect of gas-producing bacteria on plant growth, comprising: an experimental chamber and a control system. The top of the experimental chamber is open, and the experimental chamber is divided into an upper plant culture area and a lower microbial culture area by a horizontally arranged partition board. A hatch is provided on the side wall of the microbial culture area.

[0007] Planting substrate is placed at the top of the partition board.

[0008] A plurality of air holes are provided on the partition board.

[0009] An environmental simulation and regulation system is provided in the plant culture area, and a sterilization system and an environmental regulation system are provided in the microbial culture area. The environmental simulation and regulation system, the sterilization system, and the environmental regulation system are all electrically connected to the control system.

[0010] Preferably, a mounting plate is provided on the top of the microorganism culture area, and a plurality of mounting holes are provided on the mounting plate, and the mounting holes correspond to the positions of the pores;

[0011] A micro fan is installed in the installation hole, the installation hole and the air hole are connected by a transfer tube, and the micro fan is electrically connected to the control system.

[0012] Furthermore, a mounting seat is provided at the air hole on the partition, and an aeration pipe is installed in the mounting seat.

[0013] Furthermore, the mounting seat is a central hole structure and the inner wall is provided with internal threads, the bottom of the aeration pipe is provided with external threads matching the internal threads, the aeration pipe is provided with aeration holes, and the outer wall of the aeration pipe is covered with a filter.

[0014] Preferably, an air collecting hood is provided at a position corresponding to the mounting hole on the bottom surface of the mounting plate.

[0015] Preferably, the environmental simulation and control system includes a light simulation device, a precipitation simulation device and a temperature control device;

[0016] The light simulation device comprises a full-spectrum metal halide lamp installed at the top of the plant cultivation area;

[0017] The precipitation simulation device comprises a spray pipe installed below the full-spectrum metal halide lamp in the plant cultivation area and a water tank installed on the outer wall of the experimental cabin; the spray pipe and the water tank are connected by a water supply pipe, a plurality of spray heads are arranged on the spray pipe, and a spray pump is installed on the water supply pipe;

[0018] The temperature control device comprises a ventilation fan 1, and the ventilation fan 1 is arranged on the side wall of the plant cultivation area;

[0019] The full-spectrum metal halide lamp, the spray pump, and the ventilation fan are all electrically connected to the control system.

[0020] Preferably, the sterilization system comprises an ultraviolet lamp, and the ultraviolet lamp is electrically connected to the control system.

[0021] Preferably, the environmental control system includes an electric heating tube and a ventilation fan;

[0022] The bottom of the microorganism culture area is a double-layer hollow structure, and the electric heating tube is arranged in the hollow structure;

[0023] The second ventilation fan is arranged on the side wall of the microorganism culture area, and a fan cover is arranged on the outer side of the second ventilation fan;

[0024] The electric heating tube and the ventilation fan are both electrically connected to the control system.

[0025] Preferably, temperature sensors are provided in both the plant cultivation area and the microorganism cultivation area, and the temperature sensors are electrically connected to the control system.

[0026] The present invention also provides a method for conducting experiments using the device described in the above technical solution, including the following steps:

[0027] (1) Turn on the sterilization system to treat the microorganism cultivation area to make it an aseptic environment;

[0028] (2) According to the experimental cultivation requirements, place the culture medium inoculated with microorganisms in the microorganism cultivation area, close the hatch, and adjust the appropriate cultivation temperature through the environmental control system according to the temperature requirements for the cultivation of the target microorganism;

[0029] (3) Place the planting substrate suitable for the target plant on the partition board, then plant the target plant, and regulate the light, precipitation and temperature through the environmental simulation and control system according to the growth requirements of the target plant.

[0030] Through the above technical solution, it can be seen that compared with the prior art, the present invention discloses an experimental device and experimental method for studying the effect of gas-producing bacteria on plant growth, and has the following beneficial effects:

[0031] The technical solution of the present invention integrates the microorganism cultivation part and the plant planting part. The gas produced by the gas-producing bacteria can directly act on the plants without an intermediate collection link, which not only simplifies the experimental steps but also avoids errors generated during the transfer process, improving the accuracy of experimental research. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are only the embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained according to the provided drawings without creative efforts.

[0033] Figure 1 It is the external overall structure diagram of the device of the present invention;

[0034] Figure 2 It is the internal structure diagram of the device of the present invention;

[0035] In the figure, 1 - experimental cabin, 2 - control system, 3 - partition board, 4 - plant cultivation area, 5 - microorganism cultivation area, 6 - cabin door, 7 - observation window, 8 - planting substrate, 9 - full-spectrum metal halide lamp tube, 10 - lamp tube mounting seat, 11 - spray pipe, 12 - water tank, 13 - water supply pipe, 14 - spray head, 15 - spray pump, 16 - ventilation fan I, 17 - ultraviolet lamp, 18 - ultraviolet lamp mounting seat, 19 - electric heating pipe, 20 - ventilation fan II, 21 - temperature sensor, 22 - mounting plate, 23 - micro fan, 24 - adapter pipe, 25 - mounting seat, 26 - aeration pipe, 27 - gas collecting hood. Detailed implementation mode

[0036] The technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0037] As attached Figure 1-2 As described, the experimental device for studying the effect of gas-producing bacteria on plant growth includes: an experimental cabin 1 and a control system 2. The top of the experimental cabin 1 is open, and specifically, a top cabin cover can be set according to experimental needs. The experimental cabin 1 is divided into an upper plant cultivation area 4 and a lower microorganism cultivation area 5 by a horizontally arranged partition board 3. A cabin door 6 is provided on the side wall of the microorganism cultivation area 5. In order to facilitate the observation of plant cultivation, an observation window 7 can also be provided on the side wall of the plant cultivation area 4 according to actual needs;

[0038] The planting substrate 8 is placed at the top end of the partition board 3;

[0039] A plurality of air holes are provided on the partition board 3;

[0040] An environment simulation and regulation system is provided in the plant cultivation area 4, and a sterilization system and an environment regulation system are provided in the microorganism cultivation area 5. The environment simulation and regulation system, the sterilization system, and the environment regulation system are all electrically connected to the control system 2;

[0041] According to specific experimental and regulation requirements, the control system 2 can adopt a PLC control system, but is not limited thereto.

[0042] In the specific technical solution, the environment simulation and regulation system includes a light simulation device, a precipitation simulation device, and a temperature regulation device;

[0043] The light simulation device includes a full-spectrum metal halide lamp tube 9 installed at the top end inside the plant cultivation area 4. As shown in the attached drawing, for the convenience of installation, disassembly, and replacement, the full-spectrum metal halide lamp tube 9 is installed using a lamp tube mounting seat 10;

[0044] The precipitation simulation device includes a spray pipe 11 installed below the full-spectrum metal halide lamp tube 9 in the plant cultivation area 4, and a water tank 12 installed on the outer wall of the experimental chamber; the spray pipe 11 is connected to the water tank 12 by a water supply pipe 13, a plurality of spray heads 14 are arranged on the spray pipe 11, and a spray pump 15 is installed on the water supply pipe 13;

[0045] The temperature control device includes a first ventilation fan 16, and the first ventilation fan 16 is arranged on the side wall of the plant cultivation area 4;

[0046] The full-spectrum metal halide lamp tube 9, the spray pump 15, and the first ventilation fan 16 are all electrically connected to the control system 2.

[0047] The sterilization system includes an ultraviolet lamp 17, and the ultraviolet lamp 17 is electrically connected to the control system 2. As shown in the attached drawing, for the convenience of installation, disassembly and replacement, the ultraviolet lamp 17 is installed in the microorganism cultivation area 5 by an ultraviolet lamp mounting seat 18.

[0048] The environment control system includes an electric heating tube 19 and a second ventilation fan 20. A fan cover is arranged outside the second ventilation fan 20. The bottom of the microorganism cultivation area 5 is a double-layer hollow structure. The electric heating tube 19 is arranged in the hollow structure, and the second ventilation fan 20 is arranged on the side wall of the microorganism cultivation area 5;

[0049] The electric heating tube 19 and the second ventilation fan 20 are both electrically connected to the control system 2.

[0050] The action process and principle of the above technical solution device are as follows:

[0051] First, turn on the ultraviolet lamp 17 of the sterilization system to process the microorganism cultivation area 5 to make it reach a sterile environment; select the target microorganism according to the experimental cultivation requirements, and adjust the appropriate cultivation temperature through the electric heating tube 19 and the second ventilation fan 20 in the environment control system according to the temperature requirements for cultivating the target microorganism. After the temperature reaches the target, turn off the heating tube 19 and / or the second ventilation fan 20, close the fan cover, so that the microorganism cultivation area 5 has a high sealing condition, place the culture medium inoculated with the microorganism in the microorganism cultivation area 5, and close the hatch door 6. In order to improve the experimental accuracy, a sealing strip can be set at the edge of the hatch door 6; then place the planting substrate 8 suitable for the target plant on the partition plate 3, and then plant the target plant. According to the growth requirements of the target plant, the light, precipitation and temperature are regulated through the full-spectrum metal halide lamp tube 9, the spray water supply system and the first ventilation fan 16 in the environment simulation control system. Among them, the full-spectrum metal halide lamp tube 9 can generate heat during the lighting process. Therefore, no additional heating source is required in the plant planting area 4.

[0052] In some specific improvement solutions, in order to improve the accuracy of control, temperature sensors 21 are installed in both the plant cultivation area 4 and the microorganism cultivation area 5. The temperature sensors 21 are electrically connected to the control system 2. The temperature sensors 21 can accurately transmit the temperatures in the two areas to the control system 2 for timely feedback, facilitating adjustment.

[0053] In some specific improvement technical solutions, a mounting plate 22 is provided at the top of the microorganism cultivation area 5. A plurality of mounting holes are provided on the mounting plate 22, and the mounting holes correspond to the positions of the air holes.

[0054] A micro fan 23 is installed in the mounting hole 22. A transfer pipe 24 is used to connect the mounting hole 22 and the air hole 23. The micro fan 23 is electrically connected to the control system 2.

[0055] Using the micro fan 23 can accelerate the upward diffusion of the gas generated by the gas-producing microorganisms, reducing the action time; and it can also delimit the action research areas with different gas concentrations in the same experimental device by controlling the opening and closing of the micro fans 23 in different action areas and the exhaust volume, improving the practicality of the device.

[0056] In some more specific improvement solutions, a mounting seat 25 is provided at the air hole on the partition plate 3. An air distribution pipe 26 is installed in the mounting seat 25.

[0057] In some more specific improvement solutions, the mounting seat 25 has a middle hole structure and the inner wall is provided with internal threads. The bottom of the air distribution pipe 26 is provided with external threads matching the internal threads. The air distribution pipe 26 is provided with air distribution holes, and the outer wall of the air distribution pipe is coated with a filter screen.

[0058] Using the air distribution pipe 26 can penetrate into the planting substrate 8, which is more conducive to the uniform and rapid diffusion of gas in the substrate, improving the action efficiency. And it is also possible to replace the air distribution pipe according to the different effects of the gas produced by the target microorganisms on the roots or stems and leaves of the target plants. When studying the effect on the roots of the target plants, a shorter air distribution pipe can be replaced so that the gas can be evenly and rapidly diffused in the root substrate; when studying the effect on the stems and leaves of the target plants, a longer air distribution pipe can be replaced so that the gas can directly act on the stems and leaves of the plants, improving the flexibility of the research and the practicality of the experimental device.

[0059] In some more specific improvement solutions, a gas collection hood 27 is provided at the corresponding position of the mounting hole on the bottom surface of the mounting plate 22. The gas collection hood 27 can help with gas collection.

[0060] In the present specification, the various embodiments are described in a progressive manner. Each embodiment focuses on the differences from other embodiments, and the same or similar parts among the various embodiments can be referred to each other. For the apparatus disclosed in the embodiments, since it corresponds to the method disclosed in the embodiments, the description is relatively simple, and the relevant parts can be referred to the description of the method part.

[0061] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. An experimental device for studying the effect of gas-producing bacteria on plant growth, characterized in that: include: An experimental cabin and a control system, wherein the top of the experimental cabin is open, and the experimental cabin is divided into an upper plant culture area and a lower microorganism culture area by a horizontally arranged partition, and a cabin door is arranged on the side wall of the microorganism culture area; A planting matrix is ​​placed on the top of the partition; The partition is provided with a plurality of air holes; The plant culture area is provided with an environmental simulation control system, the microorganism culture area is provided with a sterilization system and an environmental control system, and the environmental simulation control system, the sterilization system and the environmental control system are all electrically connected to the control system.

2. The experimental device for studying the effect of gas-producing bacteria on plant growth according to claim 1, characterized in that: A mounting plate is provided at the top of the microorganism culture area, and a plurality of mounting holes are provided on the mounting plate, and the mounting holes correspond to the positions of the pores; A micro fan is installed in the installation hole, the installation hole and the air hole are connected by a transfer tube, and the micro fan is electrically connected to the control system.

3. The experimental device for studying the effect of gas-producing bacteria on plant growth according to claim 2, characterized in that: A mounting seat is arranged at the air hole on the partition, and an aeration pipe is installed in the mounting seat.

4. The experimental device for studying the effect of gas-producing bacteria on plant growth according to claim 3, characterized in that: The mounting seat is a middle hole structure and the inner wall is provided with internal threads, the bottom of the aeration pipe is provided with external threads matching the internal threads, the aeration pipe is provided with aeration holes, and the outer wall of the aeration pipe is covered with a filter screen.

5. The experimental device for studying the effect of gas-producing bacteria on plant growth according to claim 2, characterized in that: An air collecting hood is arranged at a position corresponding to the mounting hole on the bottom surface of the mounting plate.

6. The experimental device for studying the effect of gas-producing bacteria on plant growth according to claim 1, characterized in that: The environmental simulation and control system includes a light simulation device, a precipitation simulation device and a temperature control device; The light simulation device comprises a full-spectrum metal halide lamp installed at the top of the plant cultivation area; The precipitation simulation device comprises a spray pipe installed below the full-spectrum metal halide lamp in the plant cultivation area and a water tank installed on the outer wall of the experimental cabin; the spray pipe and the water tank are connected by a water supply pipe, a plurality of spray heads are arranged on the spray pipe, and a spray pump is installed on the water supply pipe; The temperature control device comprises a ventilation fan 1, and the ventilation fan 1 is arranged on the side wall of the plant cultivation area; The full-spectrum metal halide lamp, the spray pump, and the ventilation fan are all electrically connected to the control system.

7. The experimental device for studying the effect of gas-producing bacteria on plant growth according to claim 1, characterized in that: The sterilization system comprises an ultraviolet lamp, and the ultraviolet lamp is electrically connected to the control system.

8. The experimental device for studying the effect of gas-producing bacteria on plant growth according to claim 1, characterized in that: The second ventilation fan is arranged on the side wall of the microorganism culture area, and a fan cover is arranged on the outer side of the second ventilation fan; The bottom of the microorganism culture area is a double-layer hollow structure, and the electric heating tube is arranged in the hollow structure; The second ventilation fan is arranged on the side wall of the microorganism culture area; The electric heating tube and the ventilation fan are both electrically connected to the control system.

9. The experimental device for studying the effect of gas-producing bacteria on plant growth according to claim 1, characterized in that: Temperature sensors are arranged in the plant culture area and the microorganism culture area, and the temperature sensors are electrically connected to the control system.

10. A method for conducting an experiment using the device according to any one of claims 1 to 9, characterized in that: The following steps are involved: (1) Turn on the sterilization system to treat the microbial culture area to achieve a sterile environment; (2) Based on the experimental culture requirements, place the culture medium inoculated with microorganisms in the microorganism culture area, close the door, and adjust the appropriate culture temperature through the environmental control system based on the temperature requirements of the target microorganism culture; (3) A planting substrate suitable for the target plants is placed on the partition, and then the target plants are planted. According to the growth requirements of the target plants, the light, precipitation and temperature are regulated through the environmental simulation control system.

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