Preparation for preventing and treating internal rot of Chinese chestnuts, method and application

Abstract

The invention relates to the field of plant protection, in particular to a preparation for preventing and treating internal rot of Chinese chestnuts, a method and application. The preparation for preventing and treating internal rot of Chinese chestnuts comprises a first preparation, a second preparation and a third preparation, the first preparation contains azoxystrobin with the concentration of 2 to 4g / L; the second preparation contains pyraclostrobin with the concentration of 2 to 4 g / L; and the third preparation contains chitosan with the concentration of 1-3g / L and sodium chloride with the concentration of 1-3g / L. The preparation comprises a plurality of medicaments, different medicaments are applied in different growth periods of the Chinese chestnuts by combining the growth characteristics of the Chinese chestnuts and the morbidity characteristics of the internal rot of the Chinese chestnuts, the inhibition effect on the internal rot of the Chinese chestnuts is obvious, the intact fruit rate of chestnut kernels in the storage period of the Chinese chestnuts is increased, and the loss caused by the internal rot is reduced; a good control effect on the internal rot of the Chinese chestnuts can be achieved, especially for the internal rot of the Chinese chestnuts planted in a field.

Description

Technical Field

[0001] The invention relates to the field of plant protection, and in particular to a preparation, method and application for preventing and treating chestnut internal rot. Background Art

[0002] Chestnut internal rot is also known as chestnut kernel spot disease and chestnut fruit rot. At present, chestnuts are stored in cold storage after harvesting, and the annual internal rot rate of chestnut kernels is 7% to 10%. The notable characteristics of chestnut internal rot are: the appearance of the chestnut husk, chestnut shell and chestnut kernels do not change after the chestnuts are harvested. After the chestnuts are stored in the warehouse for 2 to 3 months, various necrotic spots will appear on the surface of the chestnut kernels, which will cause rot and mildew in severe cases. The main symptoms of chestnut kernel internal rot are: in the early stage of the disease, chalky substances are produced on the outer layer of the chestnut kernels and expand to the inner core, gradually turning brown, forming dark green or black rot spots, with bitter and moldy taste; in the later stage, the spots gradually dry up and even form cavities, causing the kernels to completely dry and harden, and gray-black mycelium layers can be seen, and the kernels become easy to crush; at the same time, powdery fruiting bodies are formed under the seed coat; over time, the diseased parts are often infected by bacteria, becoming soft and rotten, producing odors, and the kernels lose their edible value.

[0003] Different types of pathogens were isolated from the diseased chestnut kernels, among which Anthracnose, Fusarium, Alternaria, etc. were proved to be dominant pathogenic strains. It was preliminarily determined that the pathogens began to invade the stigma of the female flowers with pollen during the pollination period of chestnut female flowers in mid-June, and then entered the style and ovary. As the chestnut kernels developed, the pathogens lurked in the chestnut kernels. The process of chestnut internal rot is relatively long. It is generally believed that the involucre, style, peel and other tissues of the kernels during the growth period carry pathogens, but the kernels do not show symptoms until they are harvested. The pathogens have obvious latent infection characteristics, which proves that the onset of the pathogens is related to phenolic substances in chestnut fruit tissues. When the chestnut fruit matures, the content of phenolic substances in the peel decreases sharply, weakening the inhibitory effect on the pathogens. In addition, water loss in chestnut kernels during storage is also an important cause of internal rot. Chestnuts are stubborn seeds that have active metabolism when harvested and are highly sensitive to water loss. During the process of chestnuts being harvested when mature, sold by farmers, and transported to storage, the moisture content of the chestnut kernels decreases, the sugar content increases, the amylase activity becomes stronger, membrane peroxidation increases, the cell membrane permeability increases, and the activity of free radical scavengers superoxide dismutase and peroxidase significantly decreases, which is an important cause of internal rot.

[0004] In order to prevent and control chestnut kernel internal rot, the main methods currently adopted are spraying pesticides during the flowering period and preserving during the storage period. However, existing research is mostly limited to indoor toxicity determination, and there are relatively few studies on the prevention and control of chestnut kernel internal rot by spraying pesticides in the field. Despite this, research on spraying pesticides during the flowering period to prevent and control internal rot still needs to be further deepened, especially with the popularization of plant protection drones, this field is expected to gain more convenience and breakthroughs.

[0005] In view of this, the present invention is hereby provided. Summary of the Invention

[0006] An object of the present invention is to provide a preparation, method and application for preventing and treating chestnut internal rot disease. The preparation for preventing and treating chestnut internal rot disease has the advantages of significant inhibitory effect on chestnut internal rot disease, increasing the good fruit rate of chestnut kernels during the storage period of chestnuts, and reducing the losses caused by internal rot disease.

[0007] In order to achieve the above object of the present invention, the following technical solutions are specifically adopted:

[0008] One aspect of the present invention relates to a preparation for preventing and treating chestnut internal rot disease, including: a first preparation, a second preparation and a third preparation;

[0009] Wherein, the first preparation contains: azoxystrobin with a concentration of 2 - 4 g / L;

[0010] The second preparation contains: pyraclostrobin with a concentration of 2 - 4 g / L;

[0011] The third preparation contains: chitosan with a concentration of 1 - 3 g / L and sodium chloride with a concentration of 1 - 3 g / L.

[0012] The preparation for preventing and treating chestnut internal rot disease includes a variety of medicaments. Combining with the growth characteristics of chestnuts and the incidence characteristics of chestnut internal rot disease, different medicaments are applied at different growth stages of chestnuts, which has a significant inhibitory effect on chestnut internal rot disease, increases the good fruit rate of chestnut kernels during the storage period of chestnuts, reduces the losses caused by internal rot disease, and can achieve better prevention and treatment effects on chestnut internal rot disease, especially for chestnut internal rot disease in field planting.

[0013] Preferably, the first preparation contains: azoxystrobin with a concentration of 2.5 - 3.5 g / L.

[0014] Preferably, the second preparation contains: pyraclostrobin with a concentration of 2.5 - 3.5 g / L.

[0015] Preferably, the third preparation contains: chitosan with a concentration of 1.5 - 2.5 g / L and sodium chloride with a concentration of 1.5 - 2.5 g / L.

[0016] Preferably, the first preparation contains: adjuvants.

[0017] Preferably, the second preparation contains: adjuvants.

[0018] Preferably, the adjuvants include: aerial spraying adjuvants.

[0019] Preferably, the aerial spraying adjuvants include: Yipin rosin and / or U-Ban.

[0020] Preferably, the first preparation contains a drone spraying adjuvant with a volume concentration of 0.1% to 0.4%.

[0021] Preferably, the second preparation contains a drone spraying adjuvant with a volume concentration of 0.1% to 0.4%.

[0022] One aspect of the present invention also relates to a method for preventing and treating chestnut internal rot disease, comprising the following steps:

[0023] (a) In the early pre-blooming stage, spraying the first preparation of the preparation for preventing and treating chestnut internal rot disease on the whole chestnut plant;

[0024] (b) In the full-blooming stage, spraying the second preparation of the preparation for preventing and treating chestnut internal rot disease on the whole chestnut plant;

[0025] (c) Soaking the harvested chestnuts in the third preparation of the preparation for preventing and treating chestnut internal rot disease.

[0026] In the method for preventing and treating chestnut internal rot disease, different medicaments are sprayed on the whole plant of chestnuts in the early pre-blooming stage and the full-blooming stage of chestnuts, and the harvested chestnuts are soaked in the medicament. By treating chestnuts differently at different times, the growth of the pathogenic bacteria causing chestnut internal rot disease is inhibited, the incidence rate of chestnut internal rot disease is reduced, and the morbidity degree of chestnut internal rot disease is improved.

[0027] Preferably, the soaking treatment time is 2 to 8 minutes.

[0028] Another aspect of the present invention also relates to a chestnut planting method, including the method for preventing and treating chestnut internal rot disease.

[0029] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0030] (1) The preparation for preventing and treating chestnut internal rot disease provided by the present invention is convenient to use and includes a variety of medicaments. Combining the growth characteristics of chestnuts and the morbidity characteristics of chestnut internal rot disease, different medicaments are applied at different growth stages of chestnuts, and the inhibitory effect on chestnut internal rot disease is remarkable. It increases the good fruit rate of chestnut kernels during the storage period of chestnuts, reduces the losses caused by internal rot disease, and can achieve better prevention and treatment effects on chestnut internal rot disease, especially for chestnut internal rot disease in field planting.

[0031] (2) For the preparation for preventing and treating chestnut internal rot disease provided by the present invention, the content of the active ingredients in each medicament meets the "National Standard of the People's Republic of China - National Food Safety Standard / Limits of Contaminants in Foods" and the "National Standard of the People's Republic of China - Chestnut Quality Grade", and has the advantages of stability and safety in inhibiting chestnut internal rot disease.

[0032] (3) The method for preventing and controlling chestnut internal rot disease provided by the present invention sprays different medicaments on the whole chestnut plant during the early blooming stage and the full blooming stage of chestnut, and immerses the harvested chestnuts in the medicaments after harvesting. By treating chestnuts differently at different times, the growth of the pathogenic bacteria causing chestnut internal rot disease is inhibited, the incidence rate of chestnut internal rot disease is reduced, and the morbidity degree of chestnut internal rot disease is improved.

[0033] (4) The method for preventing and controlling chestnut internal rot disease provided by the present invention uses a drone to complete the spraying of the first preparation and the second preparation, can evenly spray the control medicament on the whole chestnut plant, reduce the dosage of the medicament, obtain stable drug efficacy, and improve the control effect and efficiency of internal rot disease. Detailed implementation manners

[0034] The technical solutions of the present invention will be clearly and completely described below in conjunction with the detailed implementation manners. However, those skilled in the art will understand that the following described embodiments are some embodiments of the present invention, rather than all embodiments, and are only used to illustrate the present invention and should not be regarded as limiting the scope of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the scope of protection of the present invention. For those conditions not specified in the embodiments, they are carried out according to the conventional conditions or the conditions recommended by the manufacturer. For the reagents or instruments not specified by the manufacturer, they are all conventional products that can be obtained through commercial purchase.

[0035] One aspect of the present invention relates to a preparation for preventing and controlling chestnut internal rot disease, including: a first preparation, a second preparation, and a third preparation;

[0036] Among them, the first preparation contains: azoxystrobin with a concentration of 2 - 4 g / L;

[0037] The second preparation contains: pyraclostrobin with a concentration of 2 - 4 g / L;

[0038] The third preparation contains: chitosan with a concentration of 1 - 3 g / L and sodium chloride with a concentration of 1 - 3 g / L.

[0039] The preparation for preventing and controlling chestnut internal rot disease is convenient to use, includes a variety of medicaments, combines the growth characteristics of chestnuts and the morbidity characteristics of chestnut internal rot disease, applies different medicaments at different growth stages of chestnuts, has a significant inhibitory effect on chestnut internal rot disease, increases the good fruit rate of chestnut kernels during the storage period of chestnuts, reduces the losses caused by internal rot disease, and can achieve a better control effect on chestnut internal rot disease, especially for chestnut internal rot disease in field planting.

[0040] For the preparation for preventing and treating chestnut internal rot, the content of the active ingredients in each medicament complies with the "National Standard of the People's Republic of China - National Food Safety Standard / Limits of Contaminants in Foods" and the "National Standard of the People's Republic of China - Chestnut Quality Grade", and has the advantages of stability and safety in inhibiting chestnut internal rot.

[0041] In some specific embodiments, the concentration of azoxystrobin in the first preparation can be, for example, but not limited to, any one of 2 g / L, 2.5 g / L, 3 g / L, 3.5 g / L or 4 g / L or the range value between any two of them. The first preparation containing a certain concentration of azoxystrobin is sprayed on the whole plant in the early stage of full bloom of chestnut. In the early stage of the invasion of the pathogenic bacteria causing internal rot, its growth activity is inhibited, thereby preventing and treating chestnut internal rot.

[0042] In some specific embodiments, the concentration of pyraclostrobin in the second preparation can be, for example, but not limited to, any one of 2 g / L, 2.5 g / L, 3 g / L, 3.5 g / L or 4 g / L or the range value between any two of them. The second preparation containing a certain concentration of pyraclostrobin is sprayed on the whole plant during the full bloom period of chestnut to further inhibit the growth of pathogenic bacteria.

[0043] In some specific embodiments, in the third preparation, the concentration of chitosan can be, for example, but not limited to, any one of 1 g / L, 1.5 g / L, 2 g / L, 2.5 g / L or 3 g / L or the range value between any two of them, and the concentration of sodium chloride can be, for example, but not limited to, any one of 1 g / L, 1.5 g / L, 2 g / L, 2.5 g / L or 3 g / L or the range value between any two of them. After harvesting, there will still be some pathogenic bacteria remaining in the chestnut kernels. Soaking in the third preparation containing a certain concentration of chitosan and sodium chloride can remove most of the pathogenic bacteria, thereby completing the final prevention and treatment means.

[0044] Furthermore, the first preparation is sprayed on the whole plant in the early stage of full bloom.

[0045] Furthermore, the second preparation is sprayed on the whole plant during the full bloom period.

[0046] Furthermore, the third preparation is used to soak the chestnuts after harvesting.

[0047] Furthermore, the first preparation also contains acceptable auxiliaries in the art, which are used to achieve better spraying effects of the first preparation.

[0048] Furthermore, the second preparation also contains acceptable auxiliaries in the art, which are used to achieve better spraying effects of the second preparation.

[0049] In order to be able to spray the preparation for preventing and controlling chestnut internal rot disease provided by the present invention through flight equipment such as unmanned aerial vehicles, in some specific embodiments, the adjuvant includes but is not limited to: aerial spraying adjuvant.

[0050] The aerial spraying adjuvant mentioned in the present invention refers to an auxiliary substance added when spraying pesticides using a plant protection unmanned aerial vehicle. It can improve the effect of unmanned aerial vehicle pesticide spraying and reduce the amount of pesticide used.

[0051] The present invention does not specifically limit the type of adjuvant, and conventional aerial spraying adjuvants in the art can be used to implement the technical solution of the present invention. In some specific embodiments, the aerial spraying adjuvant includes but is not limited to: commonly used aerial spraying adjuvants in the market such as Yipin rosin and / or U Companion.

[0052] Furthermore, the first preparation contains: an aerial spraying adjuvant with a volume concentration of 0.1% to 0.4% (for example, it can be but is not limited to any point value among 0.1%, 0.2%, 0.22%, 0.25%, 0.28%, 0.3% or 0.4% or the range value between any two of them).

[0053] Furthermore, the second preparation contains: an aerial spraying adjuvant with a volume concentration of 0.1% to 0.4% (for example, it can be but is not limited to any point value among 0.1%, 0.2%, 0.22%, 0.25%, 0.28%, 0.3% or 0.4% or the range value between any two of them).

[0054] Limiting the addition amount of the aerial spraying adjuvant in the first preparation and the second preparation within a certain range can improve the dispersibility of the two preparations, facilitate uniform spraying of the pesticide, save the dosage of the pesticide, and improve the control stability of the pesticide.

[0055] Another aspect of the present invention also relates to an indexing method for the preparation for preventing and controlling chestnut internal rot disease, including the following steps:

[0056] (a) Mix the raw materials of the first preparation and prepare them into a solution to obtain the first preparation;

[0057] (b) Mix the raw materials of the second preparation and prepare them into a solution to obtain the second preparation;

[0058] (c) Mix the raw materials of the third preparation and prepare them into a solution to obtain the third preparation.

[0059] The described preparation method is simple and easy to operate, and can be prepared according to the established concentration.

[0060] Furthermore, the solvent for preparing the first preparation includes but is not limited to: water.

[0061] Furthermore, the solvent for preparing the second preparation includes but is not limited to: water.

[0062] Furthermore, the solvent for preparing the third preparation includes but is not limited to: water.

[0063] Another aspect of the present invention also relates to a method for preventing and treating chestnut internal rot, comprising the following steps:

[0064] (a) spraying the first preparation on the whole chestnut plant in the early stage of full flowering;

[0065] (b) spraying the second preparation on the whole chestnut plant during the flowering period;

[0066] (c) soaking the harvested chestnuts in the third preparation.

[0067] The pathogen of chestnut internal rot usually begins in the pollination period of chestnut female flowers in mid-June, invades the stigma of female flowers with pollen, and enters the style and ovary. As the chestnut kernel develops, the pathogen lurks in the chestnut kernel. The method for preventing and controlling chestnut internal rot of the present invention sprays different agents on the whole plant in the early and full flowering period of chestnut, inhibits the pathogen in the early invasion of the pathogen, and achieves early prevention and control of internal rot. After harvesting, the chestnut is soaked in the agent to further remove the pathogenic bacteria in the chestnut kernel. By performing different treatments on the chestnut at different times, the growth of the pathogenic bacteria of chestnut internal rot is inhibited, the incidence of chestnut internal rot is reduced, and the incidence of chestnut internal rot is improved.

[0068] The method for preventing and controlling chestnut internal rot disease utilizes a drone to spray the first preparation and the second preparation, and can evenly spray the prevention and control agent on the entire chestnut plant, thereby reducing the dosage of the agent, obtaining a stable efficacy, and improving the prevention and control effect and efficiency of the internal rot disease.

[0069] Furthermore, the soaking time is 2 to 8 minutes, including but not limited to any one of 2 minutes, 4 minutes, 6 minutes or 8 minutes or any range between two of them. Limiting the soaking time to a certain range can ensure that the third preparation can fully exert its antibacterial effect and that the chestnut kernel will not be damaged by the agent.

[0070] Furthermore, the chestnuts after the soaking treatment were ventilated and naturally dried, and placed in a commercial chestnut cold storage (-2°C) for storage. The internal rot rate of the chestnut kernels was measured for the chestnut seeds stored for 4-5 months. 300 chestnut seeds were randomly selected for each treatment and control, and three repetitions were set. After the chestnut shells and astringent skins were removed from the chestnut fruits, the inside of the chestnut kernels were cut open, and the internal rot rate of the chestnut kernels was counted, where the internal rot rate (%) = (number of bad fruits / total number of surveys) × 100%.

[0071] The present invention does not specifically limit the spraying method of the spraying preparation, and conventional spraying methods in the art can be used to implement the technical solution of the present invention. In some specific embodiments, a drone is used to spray the first preparation and the second preparation. The drone replaces the traditional spraying method, which can not only improve the field control effect of chestnut internal rot disease, but also improve the control efficiency, providing more convenience for the control of chestnut internal rot disease.

[0072] The following will describe the implementation schemes of the present invention in detail with reference to examples. However, those skilled in the art will understand that the following examples are only used to illustrate the present invention and should not be regarded as limiting the scope of the present invention. For those not specified in the examples, the conventional conditions or the conditions recommended by the manufacturer are used. For the reagents or instruments not specified by the manufacturer, they are all conventional products that can be obtained through commercial purchase.

[0073] Example 1

[0074] The preparation for preventing and treating chestnut internal rot disease provided in this example includes: a first preparation, a second preparation, and a third preparation;

[0075] Among them, the first preparation contains: azoxystrobin with a concentration of 2 g / L and a flight prevention adjuvant with a volume concentration of 0.2%;

[0076] The second preparation contains: pyraclostrobin with a concentration of 2 g / L and a flight prevention adjuvant with a volume concentration of 0.2%;

[0077] The third preparation contains: chitosan with a concentration of 1 g / L and sodium chloride with a concentration of 1 g / L.

[0078] Example 2

[0079] The preparation for preventing and treating chestnut internal rot disease provided in this example includes: a first preparation, a second preparation, and a third preparation;

[0080] Among them, the first preparation contains: azoxystrobin with a concentration of 4 g / L and a flight prevention adjuvant with a volume concentration of 0.3%;

[0081] The second preparation contains: pyraclostrobin with a concentration of 4 g / L and a flight prevention adjuvant with a volume concentration of 0.3%;

[0082] The third preparation contains: chitosan with a concentration of 3 g / L and sodium chloride with a concentration of 3 g / L.

[0083] Example 3

[0084] The preparation for preventing and treating chestnut internal rot disease provided in this example includes: a first preparation, a second preparation, and a third preparation;

[0085] The first preparation contains: azoxystrobin at a concentration of 2.5 g / L and a drone spraying adjuvant at a volume concentration of 0.22%;

[0086] The second preparation contains: pyraclostrobin at a concentration of 2.5 g / L and a drone spraying adjuvant at a volume concentration of 0.22%;

[0087] The third preparation contains: chitosan at a concentration of 1.5 g / L and sodium chloride at a concentration of 1.5 g / L.

[0088] Example 4

[0089] The preparation for preventing and treating chestnut internal rot provided in this example includes: the first preparation, the second preparation and the third preparation;

[0090] The first preparation contains: azoxystrobin at a concentration of 3.5 g / L and a drone spraying adjuvant at a volume concentration of 0.28%;

[0091] The second preparation contains: pyraclostrobin at a concentration of 3.5 g / L and a drone spraying adjuvant at a volume concentration of 0.28%;

[0092] The third preparation contains: chitosan at a concentration of 2.5 g / L and sodium chloride at a concentration of 2.5 g / L.

[0093] Example 5

[0094] The preparation for preventing and treating chestnut internal rot provided in this example includes: the first preparation, the second preparation and the third preparation;

[0095] The first preparation contains: azoxystrobin at a concentration of 3.5 g / L and a drone spraying adjuvant at a volume concentration of 0.25%;

[0096] The second preparation contains: pyraclostrobin at a concentration of 3.5 g / L and a drone spraying adjuvant at a volume concentration of 0.25%;

[0097] The third preparation contains: chitosan at a concentration of 2 g / L and sodium chloride at a concentration of 2 g / L.

[0098] Example 6

[0099] The method for preventing and treating chestnut internal rot provided in this example includes the following steps:

[0100] (1) In the early pre-blooming stage, spray the first preparation on the whole chestnut tree;

[0101] (2) In the full-blooming stage, spray the second preparation on the whole chestnut tree;

[0102] (3) Soak the harvested chestnuts in the third preparation for 5 minutes.

[0103] Example 7

[0104] The method for preventing and controlling chestnut internal rot provided in this example is only different from that in Example 6 in that the soaking time is 2 min.

[0105] Example 8

[0106] The method for preventing and controlling chestnut internal rot provided in this example is only different from that in Example 6 in that the soaking time is 8 min.

[0107] Comparative Example 1

[0108] In the process of chestnut planting in this comparative example, no measures were taken to prevent and control internal rot (blank control).

[0109] Comparative Example 2

[0110] This comparative example is only different from Example 6 in that step 3 is omitted. The preparation for preventing and controlling chestnut internal rot in Example 5 is used.

[0111] Comparative Example 3

[0112] This comparative example is only different from Example 6 in that steps 1 and 2 are omitted. The preparation for preventing and controlling chestnut internal rot in Example 5 is used.

[0113] Comparative Example 4

[0114] This comparative example is only different from Example 6 in that step 2 is omitted. The preparation for preventing and controlling chestnut internal rot in Example 5 is used.

[0115] Comparative Example 5

[0116] This comparative example is only different from Example 5 in that sodium chloride is not added to the third preparation. The prevention and control method in Example 6 is used.

[0117] Comparative Example 6

[0118] This comparative example is only different from Example 5 in that the concentration of azoxystrobin in the first preparation is 1 g / L. The prevention and control method in Example 6 is used.

[0119] Comparative Example 7

[0120] This comparative example is only different from Example 5 in that the concentration of pyraclostrobin in the second preparation is 6 g / L. The prevention and control method in Example 6 is used.

[0121] Comparative Example 8

[0122] This comparative example is only different from Example 5 in that the concentration of sodium chloride in the third preparation is 5 g / L. The prevention and control method in Example 6 is used.

[0123] Comparative Example 9

[0124] The difference between this comparative example and Example 5 is only that the volume concentration of the aerial spraying preparation in the first preparation is 1%. The control method of Example 6 is adopted.

[0125] Comparative Example 10

[0126] The difference between this comparative example and Example 5 is only that the volume concentration of the aerial spraying preparation in the second preparation is 1%. The control method of Example 6 is adopted.

[0127] Experimental Example

[0128] The preparations for controlling chestnut internal rot provided in Examples 1 to 5 were used to control chestnuts by the method for controlling chestnut internal rot provided in Example 6, and the probability of chestnuts suffering from internal rot was statistically counted. The results are shown in Table 1.

[0129] Table 1 Comparison of different examples in the control of chestnut internal rot

[0130] Inner rot rate of chestnut (%) Example 1 1.31 ± 0.09 gf Example 2 1.44 ± 0.08 gf Example 3 1.53±0.13g Example 4 1.53±0.19g Example 5 1.21±0.11f Comparative Example 1 7.39±0.53a Comparative Example 2 3.09±0.15d Comparative Example 3 5.32±0.33b Comparative Example 4 4.18±0.24c Comparative Example 5 3.99±0.25c Comparative Example 6 2.53±0.12f Comparative Example 7 3.35±0.29d Comparative Example 8 4.03±0.31c Comparative Example 9 1.45±0.09g Comparative Example 10 1.63±0.08g

[0131] The use of the method for controlling chestnut internal rot has a significant inhibitory effect on chestnut internal rot. The incidence of internal rot has decreased by 6.18%, with an average of only 1.21%.

[0132] Although the present invention has been illustrated and described with specific examples, it should be realized that the above examples are only used to illustrate the technical solutions of the present invention, rather than to limit it; those of ordinary skill in the art should understand that: without departing from the spirit and scope of the present invention, the technical solutions described in the foregoing examples can be modified, or some or all of the technical features can be equivalently replaced; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the various embodiments of the present invention; therefore, this means that all such replacements and modifications within the scope of the present invention are included in the appended claims.

Claims

1. A preparation for preventing and treating chestnut internal rot, characterized in that: include: First, second and third preparations; Wherein, the first preparation contains: azoxystrobin at a concentration of 2 to 4 g / L; The second preparation contains: pyraclostrobin at a concentration of 2 to 4 g / L; The third preparation contains: chitosan with a concentration of 1 to 3 g / L and sodium chloride with a concentration of 1 to 3 g / L.

2. The preparation for preventing and treating chestnut internal rot according to claim 1, characterized in that: Includes at least one of the following technical features: (1) The first preparation contains: azoxystrobin at a concentration of 2.5 to 3.5 g / L; (2) the second preparation contains: pyraclostrobin at a concentration of 2.5 to 3.5 g / L; (3) The third preparation contains: chitosan at a concentration of 1.5 to 2.5 g / L and sodium chloride at a concentration of 1.5 to 2.5 g / L.

3. The preparation for preventing and treating chestnut internal rot according to claim 1, characterized in that: The first preparation contains: an auxiliary agent; And / or, the second preparation contains: an auxiliary agent.

4. The preparation for preventing and treating chestnut internal rot according to claim 3, characterized in that: The auxiliary agents include: flying control auxiliary agents.

5. The preparation for preventing and treating chestnut internal rot according to claim 4, characterized in that: The fly control auxiliary agent includes: Yipin rosin and / or Uban.

6. The preparation for preventing and treating chestnut internal rot according to claim 5, characterized in that: The first preparation contains: a fly control auxiliary agent with a volume concentration of 0.1% to 0.4%.

7. The preparation for preventing and treating chestnut internal rot according to claim 5, characterized in that: The second preparation contains: a fly control auxiliary agent with a volume concentration of 0.1% to 0.4%.

8. A method for preventing and treating chestnut internal rot, characterized in that: The following steps are involved: (a) spraying the first preparation of any one of claims 1 to 7 for preventing and treating chestnut internal rot on the whole chestnut plant in the early stage of full flowering; (b) spraying the whole chestnut plant with the second preparation of any one of claims 1 to 7 for preventing and controlling chestnut internal rot during the flowering period; (c) soaking the harvested chestnuts in the third preparation of any one of claims 1 to 7 for preventing and controlling chestnut internal rot.

9. The method for preventing and treating chestnut internal rot according to claim 8, characterized in that: The soaking time is 2 to 8 minutes.

10. A method for planting chestnuts, characterized in that: The invention comprises the method for preventing and treating chestnut internal rot as described in claim 8 or 9.