New application of lamiophlomis rotata and extract thereof

Abstract

The invention relates to the technical field of plant extracts, in particular to new application of lamiophlomis rotata and an extract thereof, and the lamiophlomis rotata and the extract thereof can be used for resisting aging, tightening, relieving, repairing and / or moisturizing.

Description

Technical Field

[0001] The present disclosure relates to the technical field of plant extracts, and in particular to new uses of Lamiophlomis lappa and its extracts. Background Art

[0002] Lamiophlomis rotata (Benth.) Kudo is a perennial herb of the genus Lamiophlomis in the family Lamiaceae, a dicotyledonous plant. It often grows on gravel beaches, river beaches or rocky meadows at an altitude of 3900-5100 meters in high mountains, and is distributed in Tibet, Qinghai, Sichuan, Gansu and other regions in my country. Lamiophlomis rotata is used as medicine with its root, rhizome or whole plant, and has multiple pharmacological activities, such as promoting blood circulation and stopping bleeding, dispelling wind and relieving pain, etc. The pharmaceutical preparations made from it are often used to treat traumatic injuries, traumatic bleeding, rheumatic arthralgia, jaundice, fractures and lumbar sprains.

[0003] As one of my country's characteristic plant resources, Lamiophlomis chinensis is one of the development hotspots of major raw material companies. In addition to conventional medicine, Lamiophlomis chinensis is currently also used to make oral care products such as toothpaste or mouthwash, using its effects of promoting blood circulation and stopping bleeding, dispelling wind and relieving pain to achieve the effect of improving gingival swelling and pain, and gingivitis. In addition, there are also cosmetics with Lamiophlomis chinensis as the active raw material. For example, the Chinese invention patent with publication number CN106619278A discloses a skin care product containing Lamiophlomis chinensis with freckle removal and whitening effects; the Chinese invention patent application with publication number CN103932970A discloses a freckle removal cream containing Lamiophlomis chinensis.

[0004] Using plant resource extracts as effective ingredients for cosmetics has always been a research hotspot in this field. However, as one of my country's characteristic plant resources, Du Yiwei is currently only used for removing spots and whitening. Its application is relatively single, and the beauty and cosmetic applications of Du Yiwei still need to be developed. Summary of the invention

[0005] In order to further develop my country's characteristic plant resources and enrich the active raw materials in the beauty industry, the present invention provides new uses of Lamiophlomis and its extracts.

[0006] In one aspect, the present disclosure provides use of Lamiophloea chinensis or an extract thereof in preparing a composition for anti-aging, firming, soothing, repairing and / or moisturizing.

[0007] The Lamiophlomis rotata extract described in the present disclosure may be an extract of the whole herb of Lamiophlomis rotata or an extract of certain parts of Lamiophlomis rotata. Specifically, the part may be selected from the above-ground part and / or the underground part. It should be understood that the above-ground part described in the present disclosure refers to any one or a mixture of combinations of stem, flower, petal, sepal, petiole, pedicel, and leaf; the underground part described in the present disclosure refers to any one or a mixture of combinations of root and rhizome. In some embodiments, the Lamiophlomis rotata extract is obtained by extracting the whole herb of Lamiophlomis rotata. In some embodiments, the Lamiophlomis rotata extract is obtained by extracting the root of Lamiophlomis rotata. In some embodiments, the Lamiophlomis rotata extract is obtained by extracting the leaf of Lamiophlomis rotata. In some embodiments, the Lamiophlomis rotata extract is obtained by mixing and extracting the root and leaf of Lamiophlomis rotata.

[0008] The Lamiophlomis rotata extract described in the present disclosure can be obtained by a variety of extraction methods, such as maceration, percolation, decoction, reflux extraction, continuous extraction, supercritical fluid extraction, enzyme extraction, steam distillation, sublimation, pressing, ultrasonic extraction, and microwave extraction. Methylshanzhiside and 8-O-acetylmethylshanzhiside are the characteristic index components of Lamiophlomis rotata. The inventors found through HPLC analysis that among the Lamiophlomis rotata extracts obtained by different extraction methods and extraction solvents, the contents of methylshanzhiside and 8-O-acetylmethylshanzhiside showed significant differences, that is, these two components were not detected in some extracts, while some extracts were significantly enriched. Combining the results of efficacy tests, although the chemical composition profiles of each Lamiophlomis rotata extract are significantly different, they all exhibit anti-aging, firming, soothing, repairing, and moisturizing effects. This confirms that methylshanzhiside and 8-O-acetylmethylshanzhiside are not the core efficacy components of the present disclosure. At the same time, regardless of the extraction method and extraction solvent used, the HPLC chromatograms of all Lamiophlomis rotata extracts show the presence of unknown characteristic peaks with the same retention time, indicating that they contain a certain unidentified specific component, which may have an important impact on the technical effects of the present disclosure, and its specific structural characterization and mechanism of action still need to be further studied. To facilitate those skilled in the art to further understand the technical content of the present disclosure, the present disclosure provides a preparation method of a Lamiophlomis rotata extract:

[0009] Take the whole herb of Lamiophlomis rotata, crush it and pass through a 40-mesh sieve, add 70% ethanol solution and extract it by ultrasonic wave for 30 minutes, then concentrate it until there is no alcohol, add an equal volume of water and stir at 60 °C for 1 hour, cool it to room temperature, filter it with diatomite, load it onto D101 resin, first wash it with water to remove impurities, then wash it with 10% ethanol solution to remove impurities, and finally elute it with 60% ethanol solution, collect the eluate, add activated carbon for decolorization for 1 hour, concentrate it, and spray dry it to obtain the product.

[0010] The Radix Angelicae Pubescentis described in the present disclosure can be directly made into a composition in the form of powder or granules, and can also be made into a composition by processing the effective ingredients obtained through processes such as pressing and fermentation. The present disclosure does not exclude the technical solution of using the effective ingredients obtained after processing Radix Angelicae Pubescentis by other processes other than extraction to prepare the relevant composition, and the above technical solution should still be regarded as falling within the scope of protection of the present disclosure.

[0011] Skin aging refers to a series of phenomena such as sagging, wrinkles, collapse, and loss of luster on the skin. There are many reasons for skin aging, including the loss of collagen and elastin. Anti-aging refers to means of combating skin aging. Common anti-aging solutions include promoting collagen synthesis and secretion, reducing collagen hydrolysis, promoting elastin synthesis and secretion, reducing elastin hydrolysis, etc. Experiments have shown that the extract of Lamiophlomis can effectively promote the synthesis and secretion of collagen by HSF cells, showing anti-aging and firming effects. Therefore, in some embodiments, Lamiophlomis or its extract is used to prepare a composition that promotes collagen synthesis.

[0012] Skin sensitivity refers to a series of conditions such as itching and redness when the skin is stimulated by external or endogenous stimuli. There are many reasons for skin sensitivity, which are related to skin nerve receptors, the integrity of skin barrier function, etc. Common methods to improve skin sensitivity include soothing and repairing. Soothing can relieve the specific manifestations of skin stimulation, such as reducing the degree of itching or pain, or reducing the degree of redness and swelling of the skin; repairing can repair the skin barrier and improve the integrity of the skin barrier to resist external invasion. Clinical experiments have shown that after using the extract of Lamiaceae, the degree of skin pain and burning caused by capsaicin has decreased significantly, and the Erythema value of the test area has also decreased significantly, showing that the extract of Lamiaceae has the effect of improving skin sensitivity problems. Therefore, in some embodiments, Lamiaceae or its extract is used to prepare a composition for improving skin sensitivity, itching, pain or redness. In addition, clinical trials have shown that the extract of Lamiophlomis odoratum can effectively repair the skin barrier and improve the skin's ability to resist external aggressions. After using the extract of Lamiophlomis odoratum, the TEWL value of the skin in the test area was significantly reduced. Therefore, in some embodiments, Lamiophlomis odoratum or its extract is used to prepare a composition for repairing the skin barrier, or for preparing a composition for improving the skin's ability to resist external aggressions.

[0013] The skin moisture content is of great significance for maintaining skin health and enhancing skin appearance. Skin with a high moisture content is smoother and more lustrous. Moisturization is a means to maintain a high skin moisture content, generally including artificially injecting moisture into the skin and reducing transepidermal water loss (TEWL), and the latter is the key to treating the root cause. Proven by clinical trials, the extract of Lamiophlomis rotata can effectively reduce transepidermal water loss and improve the skin's water-locking ability. After using the extract of Lamiophlomis rotata, the TEWL value of the skin in the test area decreased significantly, showing its efficacy in repair and moisturization. Therefore, in some embodiments, Lamiophlomis rotata or its extract is used to prepare a composition for reducing transepidermal water loss or improving the skin's water-locking ability.

[0014] The Lamiophlomis rotata or its extract of the present disclosure can be incorporated as an active ingredient into a delivery system or a sustained-release system to achieve better penetration of the active ingredient.

[0015] The term "delivery system" refers to a diluent, adjuvant, excipient, or carrier administered together with the active ingredient of the present disclosure, and they are selected from: water, oil, or surfactants, including those of petroleum origin, animal origin, plant origin, or synthetic origin, such as and not limited to peanut oil, soybean oil, mineral oil, sesame oil, castor oil, polysorbate, sorbitan ester, ether sulfate, sulfate, betaine, glucoside, maltoside, fatty alcohol, nonoxynol, poloxamer, polyethylene oxide, polyethylene glycol, dextran, glycerol, digitonin, and the like. Those of ordinary skill in the art know the diluents that can be used in different delivery systems in which the active ingredient of the present disclosure can be administered.

[0016] The term "sustained release" is used in its conventional meaning and refers to a delivery system that provides a gradual release of the active ingredient over a period of time. In some embodiments, the sustained-release system has a relatively constant release level of the active ingredient throughout the period.

[0017] Examples of the delivery system or the sustained-release system include, but are not limited to: liposomes, oleosomes, ethosomes, millimeter capsules, micron capsules, nanocapsules, nanostructured lipid carriers, sponges, inclusion complexes, niosomes, micelles, millimeter spheres, micron spheres, nanospheres, lipid spheres, micron emulsions, nanoemulsions, millimeter particles, micron particles, or nanoparticles.

[0018] In some embodiments, the dosage form of the composition includes ointment, cream, emulsion, aqueous solution, oil, gel, powder, tablet, mud, patch, film, aerosol, spray, freeze-dried preparation, or nano preparation.

[0019] In some embodiments, the mass percentage content of Lamiophlomis rotata or its extract in the composition is 0.01% - 5%.

[0020] On the other hand, based on the efficacy of Lamiophlomis rotata, the present disclosure provides a method for delaying skin aging, comprising the following steps: topically applying an effective amount of an extract of Lamiophlomis rotata or a composition containing an effective amount of an extract of Lamiophlomis rotata to the skin area to be improved; the method is for non-therapeutic purposes.

[0021] The present disclosure also provides a method for tightening the skin, comprising the following steps: topically applying an effective amount of an extract of Lamiophlomis rotata or a composition containing an effective amount of an extract of Lamiophlomis rotata to the skin area to be improved; the method is for non-therapeutic purposes.

[0022] The present disclosure also provides a method for repairing the skin, comprising the following steps: topically applying an effective amount of an extract of Lamiophlomis rotata or a composition containing an effective amount of an extract of Lamiophlomis rotata to the skin area to be improved; the method is for non-therapeutic purposes.

[0023] The present disclosure also provides a method for improving skin sensitivity, comprising the following steps: topically applying an effective amount of an extract of Lamiophlomis rotata or a composition containing an effective amount of an extract of Lamiophlomis rotata to the skin area to be improved; the method is for non-therapeutic purposes.

[0024] The present disclosure also provides a method for enhancing the ability of the skin to resist external aggression, comprising the following steps: topically applying an effective amount of an extract of Lamiophlomis rotata or a composition containing an effective amount of an extract of Lamiophlomis rotata to the skin area to be improved; the method is for non-therapeutic purposes.

[0025] The present disclosure also provides a method for enhancing the water-locking ability of the skin, comprising the following steps: topically applying an effective amount of an extract of Lamiophlomis rotata or a composition containing an effective amount of an extract of Lamiophlomis rotata to the skin area to be improved; the method is for non-therapeutic purposes.

[0026] The term "effective amount" means an amount of the extract of Lamiophlomis rotata of the present disclosure that is non-toxic but sufficient to provide the desired effect. The extract of Lamiophlomis rotata of the present disclosure is used at an effective concentration to obtain the desired effect in the compositions of the present disclosure. In some embodiments, the concentration of the extract of Lamiophlomis rotata is between 0.00000001% (by weight) and 20% (by weight) relative to the total weight of the composition; in some embodiments, the concentration of the extract of Lamiophlomis rotata is between 0.000001% (by weight) and 15% (by weight) relative to the total weight of the composition; in some embodiments, the concentration of the extract of Lamiophlomis rotata is between 0.0001% (by weight) and 10% (by weight) relative to the total weight of the composition; in some embodiments, the concentration of the extract of Lamiophlomis rotata is between 0.01% (by weight) and 5% (by weight) relative to the total weight of the composition.

[0027] The present disclosure has the following advantages and effects:

[0028] 1. The inventors of the present disclosure have conducted in-depth research and development on Lamiophlomis rotata, and found that the extract of Lamiophlomis rotata has the effects of anti-aging, firming, soothing, repairing, and moisturizing, and can be used as an active ingredient in cosmetics to exert its anti-aging, firming, soothing, repairing, and moisturizing effects. Therefore, the present disclosure not only successfully expands the application scenarios and uses of Lamiophlomis rotata, but also provides a new choice for the raw materials of plant active extracts in cosmetics.

[0029] 2. The present disclosure provides a variety of methods for skin care, providing more skin care plan options for consumers in need of skin care. Detailed implementation manners

[0030] To make the objectives, features, and advantages of the present disclosure more obvious and understandable, the present disclosure will be further described in detail below with reference to the embodiments. Obviously, the described embodiments are only a part of the embodiments of the present disclosure, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments in the present disclosure without creative efforts shall fall within the protection scope of the present disclosure.

[0031] Embodiment 1

[0032] The leaf extract of Lamiophlomis rotata is prepared by the following steps:

[0033] Take the dry leaves of Lamiophlomis rotata, crush them and pass through a 40-mesh sieve, add a 70% ethanol solution, extract by ultrasonic wave for 30 minutes, filter, concentrate under reduced pressure, and dry to obtain leaf extract A.

[0034] Embodiment 2

[0035] The leaf extract of Lamiophlomis rotata is prepared by the following steps:

[0036] Take the dry leaves of Lamiophlomis rotata, crush them and pass through a 40-mesh sieve, add an aqueous solution, reflux and extract for 2 hours, filter, and dry to obtain leaf extract B.

[0037] Embodiment 3

[0038] The root extract of Lamiophlomis rotata is prepared by the following steps:

[0039] Take the roots of Lamiophlomis rotata, crush them and pass through a 40-mesh sieve, add a 70% ethanol solution, extract by ultrasonic wave for 30 minutes, filter, concentrate under reduced pressure, and dry to obtain root extract A.

[0040] Embodiment 4

[0041] The root extract of Lamiophlomis rotata is prepared by the following steps:

[0042] Take the roots of Lamiophlomis rotata, crush them and pass through a 40-mesh sieve, add an aqueous solution, reflux and extract for 2 hours, filter, and dry to obtain root extract B.

[0043] Example 5

[0044] The whole herb extract of Lamiophlomis rotata is prepared through the following steps:

[0045] Take the whole herb of Lamiophlomis rotata, crush it and sieve through a 40-mesh sieve. After adding a 70% ethanol solution and ultrasonic extracting for 30 min, concentrate it to remove the alcohol, add an equal volume of water, stir at 60°C for 1 h, filter to obtain the filtrate, and dry it to obtain the whole herb extract A.

[0046] Example 6

[0047] The whole herb extract of Lamiophlomis rotata is prepared through the following steps:

[0048] Take the whole herb of Lamiophlomis rotata, crush it and sieve through a 40-mesh sieve. After adding a 70% ethanol solution and ultrasonic extracting for 30 min, concentrate it to remove the alcohol, add an equal volume of water, stir at 60°C for 1 h, cool it to room temperature, filter it after adding diatomite, load it onto D101 resin, first wash it with water to remove impurities, then wash it with a 10% ethanol solution to remove impurities, and finally elute it with a 60% ethanol solution. Collect the eluate, concentrate it under reduced pressure and dry it to obtain the whole herb extract B.

[0049] Example 7

[0050] The whole herb extract of Lamiophlomis rotata is prepared through the following steps:

[0051] Take the whole herb of Lamiophlomis rotata, crush it and sieve through a 40-mesh sieve. After adding a 70% ethanol solution and ultrasonic extracting for 30 min, concentrate it to remove the alcohol, add an equal volume of water, stir at 60°C for 1 h, cool it to room temperature, filter it after adding diatomite, load it onto D101 resin, first wash it with water to remove impurities, then wash it with a 10% ethanol solution to remove impurities, and finally elute it with a 60% ethanol solution. Collect the eluate, add activated carbon for decolorization for 1 h, concentrate it, and spray dry it to obtain the whole herb extract C.

[0052] The total polysaccharide and total polyphenol contents of the Lamiophlomis rotata extracts prepared in Examples 1-7 were quantitatively analyzed by ultraviolet spectrophotometry. The detection wavelength for analyzing the total polysaccharide content was 490 nm, and the detection wavelength for analyzing the total polyphenol content was 760 nm. The results are shown in Table 1.

[0053] Table 1 Total substance content of Lamiophlomis rotata extract

[0054] Group Total polysaccharide content (%) Total polyphenol content (%) Leaf extract A 0.42 17.52 Leaf extract B 21.65 4.66 Root extract A 0.71 18.79 Root extract B 49.70 2.24 Whole herb extract A 47.50 0.79 Whole herb extract B 0.80 14.83 Whole herb extract C 0.76 14.42

[0055] Example 8

[0056] The Lamiophlomis rotata emulsion is prepared according to the formula in Table 2 below:

[0057] Table 2

[0058]

[0059]

[0060] According to the formula, add the raw materials of Phase A to the main emulsifying pot, disperse them evenly, heat to 80 - 85 °C, keep warm for 30 - 35 min, and turn on the vacuum to -0.03 to -0.06 MPa during the heat preservation process. Then add the raw materials of Phase B and Phase C in sequence, and homogenize with a high-speed homogenizer at 5000 - 10000 rpm for 3 - 5 min. After complete homogenization, continuously stir to cool the mixture to 35 - 40 °C, then add the raw materials of Phase D, and stir for 15 - 20 min; add the raw materials of Phase E that have been completely dissolved in water in small amounts and multiple times, adjust the pH value to 5.5 - 6.5, and then stir for 10 - 15 min. Filter through a 200-mesh filter cloth to obtain the Duyiwei emulsion.

[0061] Example 9 Collagen III Content Test

[0062] 9.1 Reagents and Materials

[0063] PBS, complete medium, trypsin, RIPA lysis buffer, Collagen III ELISA kit, BCA protein kit.

[0064] 9.2 Instruments

[0065] CO2 incubator, laminar flow hood, microplate reader, air bath thermostatic shaker.

[0066] 9.3 Cell Lines

[0067] Human skin fibroblasts (HSF).

[0068] 9.4 Samples to be Tested

[0069] Experimental group: UV radiation, add leaf extract A, leaf extract B, root extract A, root extract B, whole herb extract A, whole herb extract B, and whole herb extract C respectively, and the test concentration is 100 ppm for all.

[0070] Blank control group (Control): PBS.

[0071] UV group: UV radiation, add PBS.

[0072] 9.5 Experimental Methods

[0073] Take HSF cells in the exponential growth phase with good condition, add 0.25% trypsin digestion solution, digest to make the adherent cells detached, count (1 - 4)×10 5 cells / mL, and make a cell suspension. Inoculate it in a 12-well plate and culture it in a CO2 incubator for 24 h.

[0074] Aspirate the complete medium in the wells. Add PBS to the experimental group and the UV group, and expose them to a UVB lamp for 15 min. Add complete medium to the blank control group without irradiation.

[0075] After 15 min of irradiation, aspirate the PBS solution. Add complete medium and the test sample to the experimental group, and add complete medium and PBS to the UV group. The experimental group, the UV group, and the blank control group are all cultured for 48 h.

[0076] After the culture is completed, collect the cells, centrifuge to obtain the cell pellet, add RIPA lysis buffer, and homogenize with a vortex mixer 3 times (30 s each time, with a 3-min interval), and then centrifuge at 12,000 rpm for 10 min. Aspirate the supernatant lysis solution for detection with an ELISA kit, and detect the total protein concentration of the supernatant lysis solution with a BCA protein quantification kit.

[0077] 9.6 Experimental results

[0078] Collagen III is the main component of the microcollagen layer, which is a tissue structure located between the epidermis and the dermis. It is the key to supporting the epidermis and also the first step in skin collapse. Therefore, promoting the synthesis of collagen III is of great significance for skin anti-aging. In an environment of excessive UV exposure, the activity of collagenase increases significantly, and the synthesis of collagen is also inhibited. In this experiment, cells irradiated with ultraviolet light were treated with the test sample, and the content of collagen III in the corresponding cells was detected to determine whether the extract of Lamiophlomis rotata of the present disclosure can promote the synthesis of collagen III.

[0079] The results of the effect of the test sample on the synthesis of collagen III are shown in Table 3.

[0080] Table 3 Results of the effect of the test sample on the synthesis of collagen III (Mean±SD)

[0081] Test group / sample Relative content of collagen III (%) Test group / sample Relative content of collagen III (%) Blank control group 100.00±6.77 UV group <![CDATA[80.58±3.25 # > Leaf extract A 111.44±10.01* Leaf extract B 117.74±8.76** Root extract A 148.70±12.76** Root extract B 149.56±25.49* Whole herb extract A 165.02±15.32** Whole herb extract B 151.14±16.60** Whole herb extract C 168.23±10.45*** / /

[0082] Note: Compared with the blank control group, # P<0.05; compared with the UV group, *P<0.05, **P<0.01, ***P<0.001

[0083] The results showed that compared with the blank control group, the content of collagen III in the UV group decreased significantly after UV irradiation; compared with the UV group, leaf extract A, leaf extract B, root extract A, root extract B, whole herb extract A, whole herb extract B, and whole herb extract C could all significantly increase the content of collagen III, proving that the extract of Lamiophlomis rotata prepared by any extraction process has a significant effect on promoting collagen synthesis, is beneficial to counteracting skin collapse, and exhibits significant anti-aging and firming effects.

[0084] Example 10 Test on the Efficacy of Soothing and Repairing Human Skin

[0085] 10.1 Test Purpose

[0086] According to the principles of random, single-blind and parallel tests, by continuously using the test samples by the subjects and combining the results of instrument detection, a comprehensive evaluation is made on the soothing and repairing efficacy of the test samples.

[0087] 10.2 Test Subjects

[0088] Subjects: A total of 32 volunteers aged 18 to 60 years old were recruited. Finally, 30 valid data were obtained, and 2 dropped out.

[0089] Inclusion criteria are as follows: male or female aged between 18 and 60 years old; capsaicin score not less than 3 points; able to cooperate well with the researcher; having a certain cognitive judgment ability and understanding the capsaicin stimulation score table (Table 4); being able to read and understand all the contents of the informed consent form and voluntarily signing the informed consent form.

[0090] Table 4 Capsaicin Stimulation Score Table (5-point scale)

[0091] Score Evaluation 0 points No feeling 1 point Barely perceptible 2 points Slightly perceptible 3 points Moderately perceptible 4 points Severely perceptible 5 points Pain perceptible

[0092] Have signed the informed consent form and voluntarily participated in this test.

[0093] Those who meet any of the following criteria will be excluded: those who have applied any topical medications to the test site in the past two months; those who have used antihistamines in the past week or immunosuppressants in the past month; those with clinically unresolved inflammatory skin diseases; insulin-dependent diabetes patients; asthma or other chronic respiratory disease patients who are receiving treatment; those who have received anti-cancer chemotherapy in the past six months; immunodeficiency or autoimmune disease patients; lactating or pregnant women; those who have had bilateral mastectomies and bilateral axillary lymph node resections; those whose test results are affected by scars, pigments, atrophy, nevus flammeus or other defects at the skin test site; those who are participating in other clinical trials; those with highly sensitive constitutions.

[0094] 10.3 Reagents, Materials and Instruments

[0095] Capsaicin: Diluted with 10% ethanol to prepare a 0.01% capsaicin solution (w / v);

[0096] Blank drug sensitivity test paper: 8 mm in diameter and 0.7 mm in thickness;

[0097] Transepidermal water loss test probe: Tewameter TM Hex;

[0098] Multifunctional skin imaging analysis system: C-CUBE.

[0099] 10.4 Samples to be tested

[0100] Sample group: Lamiophlomis rotata emulsion of Example 8;

[0101] Placebo group: Blank emulsion (obtained by replacing the whole herb extract C in the Lamiophlomis rotata emulsion with an equal mass of water).

[0102] 10.5 Experimental method

[0103] After the first visit of 32 subjects, the subjects were given test instructions and signed the informed consent form for the subjects, and finally at least 30 people were ensured to complete;

[0104] Capsaicin stimulation test method: Capsaicin test was carried out at the nasolabial groove position. Within 0 - 2 minutes, a cotton swab dipped in 10% alcohol solution was used to scrub back and forth at 1 cm of the nasolabial groove 5 times to make it adapt to the feeling of dampness and coolness. Those with discomfort were eliminated. Then a filter paper with a diameter of 8 mm was placed at a position 1 cm outside the nasolabial groove of the subject, 50 μL of 0.01% capsaicin was dropped on the filter paper, and it was placed for 3 minutes. The subject's feelings were asked, and the intensity of each perceived property of the feeling was evaluated by a 5 - point method. If the degree of burning sensation on the capsaicin side of the subject ≧ 2 points and the duration was greater than 30 seconds, it was positive for the capsaicin test, otherwise it was negative. The results were recorded in the capsaicin test record form.

[0105] The subjects visited on the 0th day, 14th day, and 28th day. Each time they visited, after washing their faces with the designated facial cleanser, their faces were dried with a lint - free absorbent dry paper towel. They sat quietly in a constant temperature and humidity room for 30 minutes. After the quiet sitting ended, the capsaicin stimulation test scores of the subjects participating in the test were carried out, and the data of TEWL value and Erythema value were collected with an instrument;

[0106] After the data of the subjects were collected on the 0th day, the test products were distributed to the subjects. The researchers gave the instructions for using the test products to the subjects and guided the subjects to try the test products on - site, covering the area to be measured completely with the test products. Starting from the second visit, the subjects needed to bring back the test products and hand them over to the researchers for inspection, and the usage situation of the subjects was recorded.

[0107] 10.6 Test results

[0108] The TEWL value is the trans - epidermal water loss value. The lower the TEWL value, the better the skin barrier and the stronger the water - locking ability. The Erythema value is the erythema index value. The smaller the Erythema value, the smaller the erythema and the less the skin irritation degree. The greater the improvement difference of the capsaicin stimulation score before and after use, the better the soothing and repairing effects. The test results are shown in Table 5.

[0109] Table 5 Test Results of the Soothing and Repairing Effects on Human Skin (Mean±SD)

[0110]

[0111] Note: Compared with the respective day 0 of each group, *P<0.05, ***P<0.001; compared with the placebo group, # P<0.05, ### P<0.001

[0112] The results showed that compared with before use, after 14 days and 28 days of using the test product, the TEWL values and capsaicin stimulation score values in the Lamiophlomis rotata group were significantly reduced, and the Erythema value was significantly reduced after 28 days; compared with the placebo group, after 14 days and 28 days of using the product, the TEWL values in the Lamiophlomis rotata group were significantly reduced, and there were extremely significant differences. The Erythema value and capsaicin score also decreased, and there was a significant difference at 28 days. The above results prove that the Lamiophlomis rotata extract has significant soothing, repairing and moisturizing effects. Using the Lamiophlomis rotata extract can effectively improve skin sensitivity, itching, pain and redness, enhance the skin's ability to resist external aggression, repair the skin barrier, reduce transepidermal water loss, and improve the skin's water-locking ability.

[0113] Example 11 Test on Skin Tightening of Human Body

[0114] 11.1 Test Purpose

[0115] According to the principles of random, single-blind and parallel tests, by continuously using the test samples by the subjects and combining the instrument detection results, a comprehensive evaluation of the tightening effect of the test samples is carried out.

[0116] 11.2 Test Subjects

[0117] Subjects: A total of 32 volunteers aged 35 to 60 years were recruited, and finally 30 valid data were obtained, with 2 dropouts.

[0118] Inclusion criteria are as follows: males or females aged between 35 and 60 years; using a skin elasticity tester, measuring 3 times at different positions on the frontal bone parts of the left and right faces respectively, with an interval of 3 minutes between each measurement, and the average value of the R2 values on both the left and right sides not higher than 65%; being able to read and understand all the contents of the informed consent form and voluntarily signing the informed consent form.

[0119] Have signed the informed consent form and voluntarily participated in this test.

[0120] Those who meet any of the following criteria will be excluded: those who have used muscle relaxants within the past six months; those who have applied any topical medications to the test site within the past two months; those who have used antihistamines within the past week or immunosuppressants within the past month; those with clinically unresolved inflammatory skin diseases; insulin-dependent diabetes patients; patients with asthma or other chronic respiratory diseases undergoing treatment; those who have received anti-cancer chemotherapy within the past six months; immunodeficiency or autoimmune disease patients; lactating or pregnant women; those who have undergone bilateral mastectomy and bilateral axillary lymph node dissection; those whose test results may be affected by scars, pigmentation, atrophy, nevus flammeus, or other defects at the skin test site; those who are participating in other clinical trials; those with highly sensitive constitutions.

[0121] 11.3 Instruments

[0122] Skin elasticity tester: Cutometer MPA580.

[0123] 11.4 Samples to be tested

[0124] Sample group: the Duyiwei emulsion of Example 8;

[0125] Placebo group: blank emulsion (obtained by replacing the whole herb extract C in the Duyiwei emulsion with an equal mass of water).

[0126] 11.5 Experimental methods

[0127] After the first visit of 32 subjects, the test instructions were given to the subjects, and the informed consent form of the subjects was signed at the same time, and finally at least 30 people were ensured to complete;

[0128] The subjects visited on the 0th day, 14th day, 28th day, and 56th day. Each time the subjects visited, after washing their faces with the designated facial cleanser, their faces were blotted dry with a lint-free absorbent paper towel. They sat quietly in a constant temperature and humidity room for 30 minutes. After the quiet sitting ended, data collection of instrument tests was carried out on the subjects participating in the test;

[0129] After the test products were distributed, the researchers gave the instructions on the use of the test products to the subjects and guided the subjects to try the test products on-site, covering the area to be measured completely with the test products. Starting from the second visit, the subjects needed to bring back the test products and hand them over to the researchers for inspection, and the product usage of the subjects was recorded.

[0130] 11.6 Test results

[0131] The R2 value, R7 value, and Q1 value of the skin elasticity coefficient can characterize the strength of facial skin elasticity. The higher the R2, R7, and Q1 values, the better the skin elasticity. The test results are shown in Table 6.

[0132] Table 6 Test results of human skin firmness (Mean±SD)

[0133]

[0134] Note: Compared with the respective Day 0, *P < 0.05, **P < 0.01, ***P < 0.001; compared with the placebo group, ## P < 0.01, ### P < 0.001.

[0135] The results of the human skin firmness test showed that compared with before use, after 14 days, 28 days, and 56 days of using the test product, the R2 value, R7 value, and Q1 value of the Lamiophlomis rotata group were all significantly increased, and the change rates at 14 days, 28 days, and 56 days were significantly different from those of the placebo group. It is proved that the Lamiophlomis rotata extract has a significant effect of improving skin elasticity and firmness. Using the Lamiophlomis rotata extract can effectively improve skin elasticity and achieve the purpose of firming the skin.

[0136] In this disclosure, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or terminal device comprising a series of elements not only includes those elements, but also includes other elements not expressly listed, or also includes elements inherent to such process, method, article or terminal device. Without further limitation, an element defined by the statement "comprising a..." does not exclude the presence of additional identical elements in the process, method, article or terminal device comprising the element.

[0137] Although specific embodiments of the present disclosure have been described for purposes of illustrative illustration, those skilled in the art can make various modifications or improvements without departing from the spirit and scope of the present disclosure. These modifications or improvements should fall within the scope of the appended claims of the present disclosure.

Claims

1. Use of Lamiophlomis lappa or its extract in preparing a composition for anti-aging, firming, soothing, repairing and / or moisturizing.

2. The use according to claim 1, characterized in that The composition is used for promoting collagen synthesis.

3. The use according to claim 1, characterized in that The composition is used to improve skin sensitivity, itching, pain or redness.

4. The use according to claim 1, characterized in that The composition is used for repairing the skin barrier, or for improving the skin's ability to resist external damage, or for reducing transepidermal water loss, or for improving the skin's ability to lock water.

5. A method for delaying skin aging, characterized in that: The following steps are involved: An effective amount of Lamiaceae extract or a composition containing an effective amount of Lamiaceae extract is topically applied to the skin area to be improved; the method is non-therapeutic.

6. A method for tightening skin, characterized in that: The following steps are involved: An effective amount of Lamiaceae extract or a composition containing an effective amount of Lamiaceae extract is topically applied to the skin area to be improved; the method is non-therapeutic.

7. A method for repairing skin, characterized in that: The following steps are involved: An effective amount of Lamiaceae extract or a composition containing an effective amount of Lamiaceae extract is topically applied to the skin area to be improved; the method is non-therapeutic.

8. A method for improving skin sensitivity, characterized in that: The following steps are involved: An effective amount of Lamiaceae extract or a composition containing an effective amount of Lamiaceae extract is topically applied to the skin area to be improved; the method is non-therapeutic.

9. A method for improving the skin's ability to resist external damage, characterized in that: The following steps are involved: An effective amount of Lamiaceae extract or a composition containing an effective amount of Lamiaceae extract is topically applied to the skin area to be improved; the method is non-therapeutic.

10. A method for improving the water-locking ability of the skin, characterized in that: The following steps are involved: An effective amount of Lamiaceae extract or a composition containing an effective amount of Lamiaceae extract is topically applied to the skin area to be improved; the method is non-therapeutic.

Images