Thuja sutchuenensis traditional Chinese medicine extract and extraction method thereof

Abstract

The invention relates to the technical field of natural plant extraction, in particular to a thuja sutchuenensis traditional Chinese medicine extract extraction method which comprises the following steps: S1, raw material treatment: selecting fresh and dry thuja sutchuenensis branches and leaves, cleaning and airing, and crushing thuja sutchuenensis materials to 60-80 meshes; s2, extracting the traditional Chinese medicine thuja sutchuenensis: step 1, extracting a solvent: a, extracting the solvent by using ethanol with the volume fraction of 70%-90% as the extracting solvent; b, an extraction process: mixing the powder and ethanol according to a solid-to-liquid ratio of 1: 8 and thuja sutchuenensis powder to an ethanol solution, placing the mixture in a water bath at 60 DEG C for reflux extraction for 2 hours, standing and cooling, filtering out supernate and reserving residues, repeatedly extracting twice, and merging extracting solutions for later use; step 2, concentrating: concentrating the extracting solution through a rotary evaporator, and controlling the water bath temperature not to exceed 50 DEG C; solvent optimization: multi-stage solvent proportion combination is used, various target components can be effectively extracted, solvent waste is reduced, and the extraction efficiency is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of natural plant extraction, and particularly relates to a Chinese herbal medicine extract of Thuja sutchuenensis Franch. and an extraction method thereof. Background Art

[0002] As a rare Chinese herbal medicine, Thuja sutchuenensis Franch. has various medicinal effects such as antioxidant, antibacterial, anti-inflammatory and anti-tumor effects;

[0003] Due to the high added value of Thuja sutchuenensis Franch. essential oil, the current extraction methods mainly include steam distillation method, supercritical CO2 extraction method, organic solvent extraction method, etc. However, the above methods, especially the supercritical CO2 extraction method and the organic solvent extraction method, have high requirements for the tightness and safety of the extraction equipment required, and also have very strict requirements for the media and process conditions used. When applied to large-scale industrial production, it may increase the equipment investment and production costs.

[0004] In addition, due to the extremely high medicinal value of Thuja sutchuenensis Franch., it has gradually been used in Chinese herbal medicine prescriptions and Chinese herbal medicine processing; however, the common Chinese herbal medicine extraction methods are mainly water extraction method or alcohol extraction method. Although they can effectively extract other components in the prescription, it is difficult to efficiently extract the volatile oil components contained in Thuja sutchuenensis Franch.

[0005] Therefore, a Chinese herbal medicine extract of Thuja sutchuenensis Franch. and an extraction method thereof are proposed. Summary of the Invention

[0006] The purpose of the present invention is to provide a Chinese herbal medicine extract of Thuja sutchuenensis Franch. and an extraction method thereof to solve the problems raised in the above background art.

[0007] To achieve the above purpose, the present invention provides the following technical solution: An extraction method of a Chinese herbal medicine extract of Thuja sutchuenensis Franch., comprising the following steps:

[0008] S1. Raw material treatment: Select fresh and dry branches and leaves of Thuja sutchuenensis Franch., wash and dry them, and crush the Thuja sutchuenensis Franch. material to 60-80 mesh;

[0009] S2. Extraction of Chinese herbal medicine of Thuja sutchuenensis Franch.:

[0010] Step 1: Solvent extraction:

[0011] a. Extraction solvent: Use ethanol with a volume fraction of 70%-90% as the extraction solvent;

[0012] b. Extraction process: According to the solid-liquid ratio of 1:8, Thuja sutchuenensis Franch. powder: ethanol solution, mix the powder with ethanol, place the mixture in a water bath at 60°C and reflux for 2 hours, let it stand and cool, filter out the supernatant and retain the residue, repeat the extraction twice, and combine the extracts for standby;

[0013] Step 2: Concentration:

[0014] Concentrate the extract by a rotary evaporator, control the water bath temperature not to exceed 50 °C, evaporate the solvent to 1 / 4 of the original volume of the solution to obtain the crude extract of Thuja sutchuenensis Franch.;

[0015] Step Three: Purification treatment:

[0016] a. Macroporous resin adsorption:

[0017] b. Use a macroporous resin column for adsorption treatment to remove non-polar impurities;

[0018] c. Gradient elution is carried out successively with deionized water and ethanol with concentrations of 30%, 50%, and 70%, and collect the ethanol elution part;

[0019] d. Separation and collection: Concentrate the collected ethanol eluate again to obtain a high-purity extract of Thuja sutchuenensis Franch.;

[0020] Step Four: Drying:

[0021] Freeze-drying: Carry out freeze-drying on the concentrated solution to finally obtain the powder of the extract of Thuja sutchuenensis Franch.;

[0022] A traditional Chinese medicine extract of Thuja sutchuenensis Franch. includes total flavonoids, polyphenols, volatile oils, triterpenoids, and other trace active substances.

[0023] Preferably, in Step One: Solvent extraction, a mixed solvent of ethanol and water is used. According to the polarity of different components, different polar components are obtained during the extraction process by gradually increasing the ethanol concentration.

[0024] Preferably, the preparation process of the mixed solvent includes:

[0025] The first step: Use an ethanol solution with a volume fraction of 50% for extraction to dissolve water-soluble components;

[0026] The second step: Use an ethanol solution with a volume fraction of 80% for extraction to enrich medium-polar components such as flavonoids and polyphenols;

[0027] The third step: Use an ethanol solution with a volume fraction of 95% to extract relatively insoluble non-polar components.

[0028] Preferably, in Step Two: Concentration, according to the thermosensitivity of the components of Thuja sutchuenensis Franch., a segmented temperature extraction method is selected to avoid the destruction of flavonoids and polyphenol components by high temperature and improve the stability of the components;

[0029] The specific steps are as follows:

[0030] The first stage: Initially extract at 40 °C for 30 minutes to extract thermosensitive components;

[0031] Second stage: Heat up to 60 °C and perform reflux extraction for 1 hour to extract components that are not easily decomposed;

[0032] Third stage: Then heat up to 80 °C and extract for 20 minutes to dissolve poorly soluble components.

[0033] Preferably, in step three: the gradient elution in the purification treatment includes setting the solvent gradient. After macroporous resin adsorption, different polar components can be obtained by gradient elution with ethanol of different concentrations;

[0034] The optimized gradient settings are as follows:

[0035] Gradient steps:

[0036] Elution with 30% ethanol: mainly remove water-soluble impurities;

[0037] Elution with 50% ethanol: extract polyphenolic components;

[0038] Elution with 70% ethanol: extract flavonoid components;

[0039] Elution with 95% ethanol: enrich non-polar components such as volatile oils.

[0040] Preferably, in step three: the gradient elution in the purification treatment includes controlling the elution flow rate, and by controlling the elution flow rate, the control of purity and yield by the flow rate can be achieved.

[0041] Preferably, for the preparation of the mixed solvent, when using a mixed solvent of ethanol and water, its polarity index P can be calculated by the mixture ratio to obtain the best extraction effect;

[0042] The mixture ratio calculation formula is:

[0043] P = C ethanol ·P ethanol +(1 - C ethanol )·P water

[0044] Where: P ethanol and P water are the polarity indices of ethanol and water respectively, with ethanol having a value of 5.2 and water having a value of 10.2, and C ethanol is the volume fraction of ethanol between 0 and 1.

[0045] Preferably, the solubility parameter is used to judge the dissolution ability of the solvent, and the solubility S of the target component satisfies the following formula:

[0046]

[0047] Where: K is the dissolution constant, ΔH is the enthalpy change of dissolution, usually regarded as a positive value, R is the gas constant, and T is the solvent temperature.

[0048] Preferably, in step two: temperature control during concentration, the relationship between the Arrhenius equation and the diffusion coefficient is used to describe the variation trend of extraction efficiency with temperature;

[0049] The Arrhenius equation describes the influence of temperature T on the chemical reaction rate constant k and is used to evaluate the extraction efficiency of heat-sensitive components such as flavonoids and polyphenols with respect to temperature control:

[0050]

[0051] where: A is the frequency factor, E a is the activation energy, R is the gas constant, and T is the absolute temperature;

[0052] Temperature has a significant influence on the diffusion rate D during the extraction process. The relationship between the diffusion coefficient D and temperature can be described by the following formula:

[0053]

[0054] where: D0 is the frequency factor of the diffusion coefficient, E d is the diffusion activation energy, and T is the temperature.

[0055] Preferably, by calculating the separation factor α and the elution distribution coefficient Kd of ethanol at different concentrations during gradient elution, the separation effect of gradient elution can be quantified;

[0056] Among them, for the separation factor, the separation effect of ethanol at different concentrations on components can be evaluated by the separation factor α:

[0057]

[0058] where: K d,1 and K d,2 are the distribution coefficients of flavonoids and polyphenols at a specific ethanol concentration, respectively;

[0059] Among them, the elution distribution coefficient:

[0060] The elution distribution coefficient K d represents the distribution of components between the macroporous resin and the solvent at a specific solvent concentration, and its definition is:

[0061]

[0062] where: C resin is the concentration of the component in the resin, and C solvent is the concentration of the component in the solvent.

[0063] Compared with the prior art, the beneficial effects of the present invention are:

[0064] 1. Solvent optimization: Using a multi-stage solvent ratio combination can effectively extract various target components, reduce solvent waste, and improve extraction efficiency.

[0065] 2. Temperature optimization: Segment temperature control significantly enhances the stability of thermosensitive components and avoids high-temperature degradation.

[0066] 3. Gradient elution: By optimizing the solvent concentration gradient and flow rate, the separation purity of flavonoid and polyphenol components is improved, ensuring the uniformity and high activity of the components in the extract.

[0067] 4. The thuja occidentalis extract obtained by this extraction method retains the main medicinal components in thuja occidentalis, has high purity and stability, is suitable for further drug research and development and production, and significantly enhances biological activity and safety. BRIEF DESCRIPTION OF THE DRAWINGS

[0068] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for use in the description of the specific embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0069] Figure 1 It is a flow chart of an extraction method for a thuja occidentalis traditional Chinese medicine extract of the present invention;

[0070] Figure 2 It is a reference table of the optimal solvent concentration of different ethanol and water ratios of the present invention;

[0071] Figure 3 It is a reference table of the extraction rates of flavonoids and polyphenols at different temperatures of the present invention;

[0072] Figure 4 It is a reference table of the elution flow rate on purity and yield of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0073] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0074] Please refer to Figures 1 to 4 , the present invention provides a technical solution:

[0075] An extraction method for a thuja occidentalis traditional Chinese medicine extract, comprising the following steps:

[0076] S1. Raw material treatment: Select fresh and dry Thuja sutchuenensis leaves and branches, wash and dry them, and crush the Thuja sutchuenensis materials to 60 - 80 mesh;

[0077] S2. Extraction of traditional Chinese medicine from Thuja sutchuenensis:

[0078] Step 1: Solvent extraction:

[0079] a. Extraction solvent: Use ethanol with a volume fraction of 70% - 90% as the extraction solvent;

[0080] b. Extraction process: According to the solid - liquid ratio of 1:8, Thuja sutchuenensis powder: ethanol solution, mix the powder with ethanol, place the mixture in a water bath at 60°C for reflux extraction for 2 hours, let it stand and cool, filter out the supernatant and retain the residue, repeat the extraction twice, combine the extracts and set aside;

[0081] Step 2: Concentration:

[0082] Concentrate the extract through a rotary evaporator, control the water bath temperature not to exceed 50°C, evaporate the solvent to 1 / 4 of the original volume of the solution to obtain the crude extract of Thuja sutchuenensis;

[0083] Step 3: Purification treatment:

[0084] a. Macroporous resin adsorption:

[0085] b. Use a macroporous resin column for adsorption treatment to remove non - polar impurities;

[0086] c. Gradient elution is carried out successively with deionized water and ethanol with concentrations of 30%, 50%, and 70%, and collect the ethanol elution part;

[0087] d. Separation and collection: Re - concentrate the collected ethanol eluate to obtain a high - purity extract of Thuja sutchuenensis;

[0088] Step 4: Drying:

[0089] Freeze - drying: Freeze - dry the concentrated solution to finally obtain the powder of Thuja sutchuenensis extract;

[0090] A traditional Chinese medicine extract of Thuja sutchuenensis includes total flavonoids, polyphenols, volatile oils, triterpenoids, and other trace active substances.

[0091] Specifically, in Step 1: Solvent extraction, a mixed solvent of ethanol and water is used. According to the polarity of different components, different polar components are obtained during the extraction process by gradually increasing the ethanol concentration.

[0092] Specifically, the preparation process of the mixed solvent includes:

[0093] The first step: Use an ethanol solution with a volume fraction of 50% for extraction to dissolve water - soluble components;

[0094] Step 2: Extract with an ethanol solution with a volume fraction of 80% to enrich medium-polar components such as flavonoids and polyphenols;

[0095] Step 3: Use an ethanol solution with a volume fraction of 95% to extract poorly soluble non-polar components.

[0096] Specifically, in the concentration in Step 2, according to the thermosensitivity of the Thuja sutchuenensis components, a segmented temperature extraction method is selected to avoid the destruction of flavonoids and polyphenols by high temperature and improve the stability of the components;

[0097] The specific steps are as follows:

[0098] The first stage: Initially extract at 40°C for 30 minutes to extract thermosensitive components;

[0099] The second stage: Raise the temperature to 60°C and reflux for 1 hour to extract components that are not easily decomposed;

[0100] The third stage: Then raise the temperature to 80°C and extract for 20 minutes to dissolve poorly soluble components.

[0101] Specifically, in the gradient elution in the purification treatment of Step 3, it includes setting the solvent gradient. After macroporous resin adsorption, different polar components can be obtained by gradient elution with ethanol at different concentrations;

[0102] The optimized gradient settings are as follows:

[0103] Gradient steps:

[0104] Elution with 30% ethanol: Mainly remove water-soluble impurities;

[0105] Elution with 50% ethanol: Extract polyphenol components;

[0106] Elution with 70% ethanol: Extract flavonoid components;

[0107] Elution with 95% ethanol: Enrich non-polar components such as volatile oils.

[0108] Specifically, in the gradient elution in the purification treatment of Step 3, it includes controlling the elution flow rate to control the purity and yield by controlling the elution flow rate.

[0109] Specifically, for the preparation of the mixed solvent, when using a mixed solvent of ethanol and water, its polarity index P can be calculated by the mixture ratio to obtain the best extraction effect;

[0110] The calculation formula for the mixture ratio is:

[0111] P = C ethanol ·P ethanol +(1 - C ethanol )·Pwater

[0112] Among them: P ethanol and P water are the polarity indices of ethanol and water respectively, with ethanol having a value of 5.2 and water having a value of 10.2, and C ethanol is the volume fraction of ethanol ranging from 0 to 1.

[0113] Specifically, the solubility parameter is used to judge the dissolution ability of the solvent, and the solubility S of the target component satisfies the following formula:

[0114]

[0115] Among them: K is the dissolution constant, ΔH is the enthalpy change of dissolution, usually regarded as a positive value, R is the gas constant, and T is the solvent temperature.

[0116] Specifically, in the second step: temperature control during concentration, the relationship between the Arrhenius equation and the diffusion coefficient is used to describe the variation trend of the extraction efficiency with temperature;

[0117] The Arrhenius equation describes the influence of temperature T on the chemical reaction rate constant k and is used to evaluate the extraction efficiency of heat-sensitive components such as flavonoids and polyphenols:

[0118]

[0119] Among them: A is the frequency factor, E a is the activation energy, R is the gas constant, and T is the absolute temperature;

[0120] Temperature has a significant influence on the diffusion rate D during the extraction process, and the relationship between the diffusion coefficient D and temperature can be described by the following formula:

[0121]

[0122] Among them: D0 is the frequency factor of the diffusion coefficient, E d is the diffusion activation energy, and T is the temperature.

[0123] Specifically, by calculating the separation factor α and the elution distribution coefficient Kd of ethanol with different concentrations in gradient elution, the separation effect of gradient elution can be quantified;

[0124] Among them, for the separation factor, the separation effect of ethanol with different concentrations on the components can be evaluated by the separation factor α:

[0125]

[0126] Among them: K d,1 and K d,2 are the distribution coefficients of flavonoids and polyphenols at a specific ethanol concentration respectively;

[0127] Among them, the elution distribution coefficient:

[0128] The elution distribution coefficient K d Indicates the distribution of components between the macroporous resin and the solvent at a specific solvent concentration, and its definition is:

[0129]

[0130] Where: C resin Is the concentration of the component in the resin, and C solvent Is the concentration of the component in the solvent.

[0131] Examples and experimental data

[0132] Example: Detection of total flavonoid content in Thuja sutchuenensis

[0133] 1. Experimental conditions:

[0134] Sample: 1 g of Thuja sutchuenensis extract powder

[0135] Reagent: 70% ethanol solution

[0136] Detection method: Ultraviolet spectrophotometry (UV method)

[0137] 2. Results:

[0138] Flavonoid content: Each gram of extract contains 120 mg of total flavonoids.

[0139] Extraction efficiency: After optimizing the process, the extraction rate of total flavonoid components reaches 85%

[0140] The optimal solvent concentration, extraction efficiency and component purity data at different ethanol-water ratios were determined through experiments as Figure 2 shown. The best extraction rates of flavonoids and polyphenols are obtained using an ethanol concentration of 80%. Through experimental optimization, controlling the solvent concentration at 80% can reduce the solvent consumption during the extraction process, save costs and ensure the dissolution efficiency of active ingredients

[0141] The experiments analyzed the effects of different temperatures on the extraction rates of flavonoids and polyphenols, and the results are as Figure 3 shown;

[0142] The experimental results show that the extraction rates of flavonoids and polyphenols are the highest under stepwise extraction at 40 °C and 60 °C, avoiding the degradation of heat-sensitive components caused by high temperatures. It is recommended to control the temperature below 60 °C during the extraction process to maximize the extraction rate of active ingredients;

[0143] The experiments show that the elution flow rate has an important impact on purity and yield. Through experiments at different flow rates, it is obtained as Figure 4 shown that controlling the flow rate at 3 mL / min can achieve high-purity separation while improving the elution efficiency

[0144] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for extracting a Thuja chinensis Chinese medicine extract, characterized in that: The steps include: S1, raw material processing, select fresh and dry thuja branches and leaves, wash and dry them, and crush the thuja materials into 60-80 mesh; S2. Extraction of Thuja sutchuenensis from traditional Chinese medicine: Step 1: Solvent Extraction: a. Extraction solvent: Use 70%-90% ethanol by volume as the extraction solvent; b. Extraction process: According to the solid-liquid ratio of 1:8, Thuja powder: ethanol solution, the powder was mixed with ethanol, the mixture was placed in a 60 ° C water bath for reflux extraction for 2 hours, allowed to stand and cool, the supernatant was filtered out and the residue was retained, the extraction was repeated twice, and the extracts were combined and set aside; Step 2: Concentration: The extract was concentrated by a rotary evaporator, the water bath temperature was controlled not to exceed 50°C, and the solvent was evaporated to 1 / 4 of the volume of the original solution to obtain a crude extract of Thuja sutchuenensis; Step 3: Purification: a. Macroporous resin adsorption: b. Use macroporous resin columns for adsorption treatment to remove non-polar impurities; c. Use deionized water and 30%, 50%, and 70% ethanol in sequence for gradient elution, and collect the ethanol elution portion; d. separation and collection: the collected ethanol eluate is concentrated again to obtain a high-purity Thuja extract; Step 4: Drying: Freeze drying: freeze drying the concentrated solution to finally obtain Thuja juniper extract powder; A Thuja sutchuenensis Chinese medicinal extract comprises total flavonoids, polyphenols, volatile oils, triterpenes and other trace active substances.

2. The method for extracting the Thuja sutchuenensis Chinese medicinal extract according to claim 1, wherein: The step 1: in the solvent extraction, a mixed solvent of ethanol and water is used, and according to the polarity of different components, the ethanol concentration is gradually increased to obtain components of different polarity during the extraction process.

3. The method for extracting the Thuja sutchuenensis Chinese medicinal extract according to claim 2, wherein: The mixed solvent preparation process includes: Step 1: Use 50% ethanol solution to extract and dissolve the water-soluble components; Step 2: Extract with 80% ethanol solution to enrich the medium polar components of flavonoids and polyphenols; Step 3: Use 95% by volume ethanol solution to extract the non-polar components that are difficult to dissolve.

4. The method for extracting the Thuja sutchuenensis Chinese medicinal extract according to claim 3, wherein: The second step: during the concentration, according to the heat sensitivity of the components of Thuja sutchuenensis, a segmented temperature extraction method is selected to avoid the destruction of flavonoids and polyphenols by high temperature, thereby improving the stability of the components; The specific steps are as follows: Phase 1: preliminary extraction at 40°C for 30 min to extract heat-sensitive components; The second stage: heating to 60°C, reflux extraction for 1 hour to extract components that are not easy to decompose; The third stage: the temperature is raised to 80°C and extracted for 20 minutes to dissolve the insoluble components.

5. The method for extracting the Thuja sutchuenensis Chinese medicinal extract according to claim 4, wherein: The step 3: gradient elution in the purification process includes setting a solvent gradient. After adsorption by the macroporous resin, components of different polarities can be obtained by gradient elution with ethanol of different concentrations; The optimization gradient settings are as follows: Gradient steps: 30% ethanol elution: mainly removes water-soluble impurities; 50% ethanol elution: extract polyphenols; 70% ethanol elution: extract flavonoid components; Elution with 95% ethanol: enrichment of non-polar components such as volatile oils.

6. The method for extracting the Thuja sutchuenensis Chinese medicinal extract according to claim 5, wherein: The step three: gradient elution in the purification process includes elution flow rate control, which controls the elution flow rate to achieve control of the purity and yield by the flow rate.

7. The method for extracting the Thuja sutchuenensis Chinese medicinal extract according to claim 3, wherein: When the mixed solvent is prepared using a mixed solvent of ethanol and water, its polarity index P can be calculated by the mixture ratio to obtain the best extraction effect; The mixture ratio calculation formula is: P=C ethanol ·P ethanol +(1-C ethanol )·P water Where: P ethanol and P water are the polarity indices of ethanol and water, with values ​​of 5.2 for ethanol and 10.2 for water, respectively. ethanol The volume fraction of ethanol is between 0 and 1.

8. The method for extracting the Thuja sutchuenensis Chinese medicinal extract according to claim 3, wherein: The solubility parameter determines the solubility of the solvent, and the solubility S of the target component satisfies the following formula: Where: K is the solubility constant, ΔH is the enthalpy change of dissolution, which is usually considered a positive value, R is the gas constant, and T is the solvent temperature.

9. The method for extracting the Thuja sutchuenensis Chinese medicinal extract according to claim 3, wherein: The second step: temperature control during concentration, using the relationship between the Arrhenius equation and the diffusion coefficient to describe the variation trend of the extraction efficiency with temperature; The Arrhenius equation describes the effect of temperature T on the chemical reaction rate constant k and is used to evaluate the extraction efficiency of temperature-controlled heat-sensitive components such as flavonoids and polyphenols: Where: A is the frequency factor, E a is the activation energy, R is the gas constant, and T is the absolute temperature; Temperature has a significant effect on the diffusion rate D during the extraction process. The relationship between the diffusion coefficient D and temperature can be described by the following formula: Where: D0 is the frequency factor of the diffusion coefficient, E d is the diffusion activation energy and T is the temperature.

10. The method for extracting the Thuja sutchuenensis Chinese medicinal extract according to claim 1, characterized in that: By calculating the separation factor α and elution distribution coefficient Kd of different concentrations of ethanol in gradient elution, the separation effect of gradient elution can be quantified; The separation factor, the separation effect of different concentrations of ethanol on the components can be evaluated by the separation factor α: Where: K d,1 and K d,2 are the partition coefficients of flavonoids and polyphenols at specific ethanol concentrations, respectively; The elution distribution coefficient is: Elution partition coefficient K d It indicates the distribution of components between macroporous resin and solvent at a specific solvent concentration, and is defined as: Where: C resin is the concentration of the component in the resin, C solvent is the concentration of the component in the solvent.

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