Traditional Chinese medicine compound capable of inhibiting bacteria, tightening and promoting vaginal repair as well as preparation method and preparation of traditional Chinese medicine compound

Abstract

The invention provides a traditional Chinese medicine compound capable of inhibiting bacteria, tightening and promoting vaginal repair as well as a preparation method and a preparation of the traditional Chinese medicine compound. The preparation method comprises the following steps: performing supercritical CO2 extraction on radix arnebiae seu lithospermi and saffron crocus to obtain radix arnebiae seu lithospermi-saffron crocus extract liquor; performing dynamic countercurrent extraction on the chrysanthemum and vitex agnus-castus powder, filtering, and collecting filtrate; adding pollen typhae powder into the filtrate, performing ultrasonic-assisted extraction, filtering, and collecting a composite extracting solution; mixing cortex phellodendri, radix sophorae flavescentis, radix angelicae sinensis and herba euphorbiae tripsaci after being soaked in the yellow wine, adding water, inoculating a composite strain which is composed of lactobacillus plantarum and bacillus subtilis subspecies, fermenting and filtering to obtain fermentation liquor; after sterilization, the traditional Chinese medicine compound is prepared from the radix arnebiae seu lithospermi-pollen typhae-saffron crocus extracting solution and the compound extracting solution. The composite traditional Chinese medicine liquid prepared from the traditional Chinese medicine compound can obviously improve the vaginal health index and obviously improve the vaginal relaxation degree at the same time. And the antibacterial agent has a good antibacterial effect on candida albicans and staphylococcus aureus.

Description

Technical Field

[0001] The present invention relates to the field of Chinese medicine preparations, and in particular to a Chinese medicine compound having the functions of inhibiting bacteria, tightening elasticity and promoting vaginal repair, and a preparation method and a preparation thereof. Background Art

[0002] Currently, gynecological diseases such as vaginitis and cervicitis are on the rise. Western medicine generally uses antibiotics for treatment, but it is easy to cause drug resistance, dysbacteriosis and mucosal damage. Recurrent candidal vaginitis is often related to excessive use of antibacterial drugs that damage the microecology. Although traditional Chinese medicine lotions have antibacterial effects, they have problems such as poor permeability of active ingredients and single action, making it difficult to simultaneously achieve mucosal repair and tissue elasticity recovery. It is of great significance to develop a Chinese medicine compound with antibacterial, firming and vaginal repair-promoting properties and its preparation method and preparation. Summary of the invention

[0003] In view of this, the present invention proposes a Chinese medicine compound having the functions of antibacterial, firming and promoting vaginal repair, and a preparation method and a preparation thereof, to solve the above problems.

[0004] The technical solution of the present invention is achieved as follows: a method for preparing a traditional Chinese medicine composite having antibacterial and elastic properties and promoting vaginal repair, comprising the following steps:

[0005] (1) extracting lithospermum officinale and saffron with supercritical CO2 to obtain lithospermum officinale-saffron extract;

[0006] (2) crushing chrysanthemum and vitex agnus-castus after vacuum drying; extracting chrysanthemum and vitex agnus-castus powder by dynamic countercurrent, filtering, and collecting the filtrate; crushing pollen typhae, adding pollen typhae powder to the filtrate, performing ultrasonic-assisted extraction, filtering, and collecting the composite extract;

[0007] (3) soaking Phellodendron amurense, Sophora flavescens, and Angelica sinensis in rice wine; mixing the Phellodendron amurense, Sophora flavescens, and Angelica sinensis soaked in rice wine with Herba Tripterygii, adding purified water, inoculating a composite bacterial strain consisting of Lactobacillus plantarum and Bacillus subtilis subsp. subtilis, fermenting, filtering, and obtaining a fermentation liquid; after pasteurization, mixing the fermentation liquid with an extract of Lithospermum officinale-Puhuang-Saffron and a composite extract to obtain a traditional Chinese medicine complex.

[0008] Furthermore, in step (1), the supercritical CO2 extraction temperature is 40-45°C, the pressure is 20-30 MPa, and the extraction time is 2-6 hours.

[0009] Furthermore, in step (2), the ultrasonic-assisted extraction frequency is 35 to 45 kHz, the power is 250 to 350 W, and the time is 15 to 25 minutes.

[0010] Furthermore, in step (2), the dynamic countercurrent extraction temperature is 60-70° C., the extraction is cyclically performed 2-4 times, and the extraction solvent is 25-35% v / v ethanol solution.

[0011] Furthermore, in step (3), the soaking time in rice wine is 20 to 40 minutes.

[0012] Furthermore, in step (3), the amount of purified water added is 7 to 9 times the total weight of the Phellodendron chinense, Sophora flavescens, Angelica sinensis and Three Immortal Spear herbs, the inoculation amount of the composite strain is 2 to 4% of the total weight of the Phellodendron chinense, Sophora flavescens, Angelica sinensis and Three Immortal Spear herbs, the composite strain is composed of Lactobacillus plantarum and Bacillus subtilis subsp. subtilis in a mass ratio of 1.5 to 2.5:0.8 to 1.2, the fermentation temperature is 30 to 37° C., and the fermentation time is 48 to 72 hours.

[0013] A further preparation method comprises the following raw materials in parts by weight: 15-20 parts of Phellodendron amurense, 10-15 parts of Sophora flavescens, 8-12 parts of Chrysanthemum, 8-12 parts of Lithospermum officinale, 5-10 parts of Pollen Typhae, 10-15 parts of Angelica sinensis, 3-5 parts of Saffron, 6-10 parts of Tripterygium wilfordii, and 5-8 parts of Vitex agnus-castus.

[0014] A traditional Chinese medicine compound with the functions of antibacterial, firming and promoting vaginal repair is prepared by any preparation method of the present invention.

[0015] A traditional Chinese medicine compound preparation with the functions of inhibiting bacteria, tightening elasticity and promoting vaginal repair is prepared from the traditional Chinese medicine compound of the invention.

[0016] The invention discloses a method for preparing a Chinese medicine composite preparation with the functions of antibacterial, firming and promoting vaginal repair, comprising the following steps: adding chitosan quaternary ammonium salt and sodium hyaluronate to the Chinese medicine composite, wherein the amount of chitosan quaternary ammonium salt added is 0.08% to 0.12% of the weight of the Chinese medicine composite, and the amount of sodium hyaluronate added is 0.8% to 1.2% of the weight of the Chinese medicine composite; filtering to obtain the target Chinese medicine composite preparation.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] The present invention uses Phellodendron chinense, Sophora flavescens, Chrysanthemum, Lithospermum officinale, Pollen Typhae, Angelica sinensis, Saffron, Herba Trichosanthis, and Vitex agnus-flowered as raw materials, adopts supercritical CO2 extraction, dynamic countercurrent extraction, combines ultrasonic-assisted extraction, adopts specific composite bacteria for fermentation, and prepares a Chinese medicine compound. The composite Chinese medicine liquid prepared by the Chinese medicine compound can significantly improve the vaginal health index and significantly improve the degree of vaginal relaxation. Moreover, the Chinese medicine compound liquid of the present invention has a good antibacterial effect on Candida albicans and Staphylococcus aureus, and takes effect quickly.

[0019] The traditional Chinese medicine complex of the present invention can renew the vaginal wall cells. By activating the metabolic function of the vaginal wall cells, it accelerates the natural shedding process of old cells, and at the same time promotes the growth of new cell tissues, making the internal vaginal tissues more firm and full, and realizing the regeneration of vaginal tissues. Using the traditional Chinese medicine complex of the present invention enables the continuous renewal of the vaginal mucosal layer cells. Under the stimulation, the original cells fall off, and at the same time promotes the proliferation of new muscle fibers and mucosal tissues. Finally, the effect of enriching and tightening the inner vaginal wall tissue is achieved, which can effectively improve the elasticity and tightness of the vaginal tissue. By promoting cell metabolism, new healthy tissues are generated on the vaginal wall, thereby achieving the effect of enhancing vaginal contractility and improving the internal environment. The traditional Chinese medicine complex of the present invention can effectively improve the relaxed state of the vaginal wall by promoting vaginal muscle contraction and elastic fiber repair, and relieve symptoms such as discomfort during sexual life caused thereby. Detailed implementation manners

[0020] To better understand the technical content of the present invention, specific examples are provided below to further illustrate the present invention.

[0021] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0022] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can all be obtained from commercial channels.

[0023] The bacterial strains used in the present invention are all purchased from a preservation center: Lactobacillus plantarum with a preservation number of CCTCC AB2010210; Bacillus subtilis subsp. subtilis with a preservation number of GDMCC NO.1.557.

[0024] Example 1

[0025] A traditional Chinese medicine complex with antibacterial, firming and elastic properties and promoting vaginal repair, comprising the following raw materials in parts by weight: 18 parts of Phellodendron amurense, 12 parts of Sophora flavescens, 10 parts of Chrysanthemum morifolium, 10 parts of Lithospermum erythrorhizon, 8 parts of Typha angustifolia, 12 parts of Angelica sinensis, 4 parts of Crocus sativus, 8 parts of Rhynchosia minima, 7 parts of Vitex agnus-castus.

[0026] The preparation process of the above traditional Chinese medicine complex preparation:

[0027] (1) Lithospermum erythrorhizon and Crocus sativus are extracted by supercritical CO2 for 3 h (temperature 40 °C, pressure 25 MPa) to obtain a Lithospermum erythrorhizon-Crocus sativus extract;

[0028] (2) Chrysanthemum and Vitex agnus-castus are dried under low-temperature vacuum (45°C) and then ground into fine powder of 80 mesh; the powders of chrysanthemum and Vitex agnus-castus are subjected to dynamic countercurrent extraction: circular extraction is carried out at 65°C for 3 times (the solvent is 30% v / v ethanol solution), and the extraction time for each time is 30 min, and the filtrates are combined; Pollen Typhae is ground, and the Pollen Typhae powder is added to the filtrate, and ultrasonic-assisted extraction (frequency 40 kHz, power 300 W) is carried out for 20 minutes, filtered, and the composite extract is collected;

[0029] (3) Cortex Phellodendri, Sophora flavescens, and Angelica sinensis are soaked in yellow rice wine for 30 minutes; after soaking in yellow rice wine, Cortex Phellodendri, Sophora flavescens, and Angelica sinensis are mixed with Rhynchosia dielsii, 8 times the weight of purified water is added, and a composite strain is inoculated (the inoculation amount is 3.0% of the total weight of Cortex Phellodendri, Sophora flavescens, Angelica sinensis and Rhynchosia dielsii medicinal materials). The composite strain is prepared from Lactobacillus plantarum and Bacillus subtilis subsp. subtilis according to a mass ratio of 2:1, and fermented at 35°C for 48 hours; after the fermentation broth is pasteurized, it is combined with the Lithospermum erythrorhizon-Pollen Typhae-Crocus sativus extract and the composite extract to obtain a traditional Chinese medicine complex;

[0030] (4) Chitosan quaternary ammonium salt and sodium hyaluronate are added to the traditional Chinese medicine complex. The addition amount of chitosan quaternary ammonium salt is 0.1% of the weight of the traditional Chinese medicine complex, and the addition amount of sodium hyaluronate is 1.0% of the weight of the traditional Chinese medicine complex. After sterilization through a 0.22 μm microporous filter membrane, a traditional Chinese medicine composite liquid is obtained; it is sub-packed in brown light-proof bottles and stored sealed with nitrogen filling.

[0031] Example 2

[0032] A traditional Chinese medicine complex with antibacterial and elastic properties and promoting vaginal repair, comprising the following raw materials in parts by weight: 18 parts of Cortex Phellodendri, 12 parts of Sophora flavescens, 10 parts of chrysanthemum, 10 parts of Lithospermum erythrorhizon, 8 parts of Pollen Typhae, 12 parts of Angelica sinensis, 4 parts of Crocus sativus, 8 parts of Rhynchosia dielsii, 7 parts of Vitex agnus-castus.

[0033] The preparation process of the above traditional Chinese medicine complex preparation:

[0034] (1) Lithospermum erythrorhizon and Crocus sativus are extracted by supercritical CO2 for 3 h (temperature 45°C, pressure 20 MPa) to obtain Lithospermum erythrorhizon-Crocus sativus extract;

[0035] (2) Chrysanthemum and Vitex agnus-castus are dried under low-temperature vacuum (45°C) and then ground into fine powder of 80 mesh; the powders of chrysanthemum and Vitex agnus-castus are subjected to dynamic countercurrent extraction: circular extraction is carried out at 60°C for 3 times (the solvent is 35% v / v ethanol solution), and the extraction time for each time is 30 min, and the filtrates are combined; Pollen Typhae is ground, and the Pollen Typhae powder is added to the filtrate, and ultrasonic-assisted extraction (frequency 35 kHz, power 250 W) is carried out for 25 minutes, filtered, and the composite extract is collected;

[0036] (3) soaking Phellodendron amurense, Sophora flavescens, and Angelica sinensis in rice wine for 20 minutes; mixing the Phellodendron amurense, Sophora flavescens, Angelica sinensis, and Herba Trichosanthis after being soaked in rice wine, adding 7 times the weight of purified water, and inoculating a composite strain (the inoculation amount is 2.0% of the total weight of Phellodendron amurense, Sophora flavescens, Angelica sinensis, and Herba Trichosanthis), wherein the composite strain is prepared from Lactobacillus plantarum and Bacillus subtilis subsp. subtilis in a mass ratio of 1.5:1, and fermenting at 37° C. for 48 hours; after pasteurization, the fermentation liquid is mixed with an extract of Lithospermum officinale-Puhuang-Saffron and a composite extract to obtain a Chinese medicine composite;

[0037] (4) Add chitosan quaternary ammonium salt and sodium hyaluronate to the traditional Chinese medicine complex, wherein the amount of chitosan quaternary ammonium salt added is 0.1% of the weight of the traditional Chinese medicine complex, and the amount of sodium hyaluronate added is 1.0% of the weight of the traditional Chinese medicine complex. Sterilize the mixture through a 0.22 μm microporous filter membrane to obtain a traditional Chinese medicine complex liquid; dispense the mixture into brown light-proof bottles, and store them in sealed containers filled with nitrogen.

[0038] Example 3

[0039] A traditional Chinese medicine compound with the functions of inhibiting bacteria, tightening elasticity and promoting vaginal repair comprises the following raw materials in parts by weight: 18 parts of phellodendron, 12 parts of sophora flavescens, 10 parts of chrysanthemum, 10 parts of lithospermum officinale, 8 parts of pollen cattail, 12 parts of angelica sinensis, 4 parts of saffron, 8 parts of trichosanthes kirilowii and 7 parts of vitex agnus-castus.

[0040] The preparation process of the above-mentioned Chinese medicine compound preparation:

[0041] (1) extracting lithospermum erythrorhizon and saffron with supercritical CO2 for 3 h (temperature 40°C, pressure 30 MPa) to obtain lithospermum erythrorhizon-saffron extract;

[0042] (2) Chrysanthemum and Vitex agnus-castus were vacuum dried at low temperature (45°C) and then crushed into 80 mesh fine powder; the chrysanthemum and Vitex agnus-castus powder were subjected to dynamic countercurrent extraction: 70°C cyclic extraction for 3 times (the solvent was 25% v / v ethanol solution), each extraction time was 30 minutes, filtered, and the filtrate was combined; Pollen Typhae was crushed, the Pollen Typhae powder was added to the filtrate, ultrasonic assisted extraction (frequency 45kHz, power 350W) was performed for 15 minutes, filtered, and the composite extract was collected;

[0043] (3) soaking Phellodendron amurense, Sophora flavescens, and Angelica sinensis in rice wine for 40 minutes; mixing the Phellodendron amurense, Sophora flavescens, Angelica sinensis, and Herba Trichosanthis after being soaked in rice wine, adding 9 times the weight of purified water, and inoculating a composite strain (the inoculation amount is 4.0% of the total weight of Phellodendron amurense, Sophora flavescens, Angelica sinensis, and Herba Trichosanthis), wherein the composite strain is prepared from Lactobacillus plantarum and Bacillus subtilis subsp. subtilis in a mass ratio of 2.5:1, and fermenting at 30° C. for 72 hours; after pasteurization, the fermentation liquid is mixed with an extract of Lithospermum officinale-Puhuang-Saffron and a composite extract to obtain a traditional Chinese medicine composite;

[0044] (4) Add chitosan quaternary ammonium salt and sodium hyaluronate to the traditional Chinese medicine complex. The addition amount of chitosan quaternary ammonium salt is 0.1% of the weight of the traditional Chinese medicine complex, and the addition amount of sodium hyaluronate is 1.0% of the weight of the traditional Chinese medicine complex. Sterilize through a 0.22 μm microporous filter membrane to obtain the traditional Chinese medicine compound solution; dispense it into brown light-proof bottles, and store it sealed with nitrogen filling.

[0045] Comparative Example 1

[0046] The difference from Example 1 is that the preparation method of the traditional Chinese medicine complex includes the following steps: crush all traditional Chinese medicinal materials, mix them, add 10 times the weight of purified water, extract by water decoction, repeat 3 times, 2 hours each time, combine the extraction solutions to obtain the traditional Chinese medicine complex; add chitosan quaternary ammonium salt and sodium hyaluronate to the traditional Chinese medicine complex. The addition amount of chitosan quaternary ammonium salt is 0.1% of the weight of the traditional Chinese medicine complex, and the addition amount of sodium hyaluronate is 1.0% of the weight of the traditional Chinese medicine complex. Sterilize through a 0.22 μm microporous filter membrane; dispense it into brown light-proof bottles, and store it sealed with nitrogen filling.

[0047] Comparative Example 2

[0048] The difference from Example 1 is that only a single strain: Lactobacillus plantarum is added, and Bacillus subtilis subsp. is not added. That is, Lactobacillus plantarum is inoculated (the inoculation amount is 3.0% of the total weight of Cortex Phellodendri, Sophora flavescens, Angelica sinensis, and Herba Coptidis).

[0049] Comparative Example 3

[0050] The difference from Example 1 is that only a single strain: Bacillus subtilis subsp. is added, and Lactobacillus plantarum is not added. That is, Bacillus subtilis subsp. is inoculated (the inoculation amount is 3.0% of the total weight of Cortex Phellodendri, Sophora flavescens, Angelica sinensis, and Herba Coptidis).

[0051] Comparative Example 4

[0052] The difference from Example 1 is that dynamic countercurrent extraction is not used in step (2). The specific treatment in step (2): Chrysanthemum morifolium and Vitex agnus-castus are dried under low temperature vacuum (45 °C) and then crushed into 80-mesh fine powder; Pollen Typhae is crushed, and Pollen Typhae powder is mixed with Chrysanthemum morifolium and Vitex agnus-castus, and ultrasonic-assisted extraction (frequency 45 kHz, power 350 W) is carried out for 15 minutes. The extraction solvent is 25% v / v ethanol solution, and the mixture is filtered to collect the composite extraction solution.

[0053] Comparative Example 5

[0054] The difference from Example 1 is that supercritical CO2 extraction is not used in step (1), but reflux extraction is used. The reflux extraction solvent is 70% v / v ethanol solution, the reflux extraction temperature is 90 °C, and the reflux extraction time is 10 h.

[0055] Test Example 1

[0056] Test samples: Traditional Chinese medicine compound liquids prepared in Examples 1 to 3 and Comparative Examples 1 to 5.

[0057] Bacterial strains: Candida albicans and Staphylococcus aureus, both purchased from the China General Microbiological Culture Collection Center.

[0058] Inoculate the bacterial strains on the inclined plane of the culture medium. After cultivation, wash the bacterial growth on the inclined plane with PBS (phosphate buffer solution), mix evenly, and prepare a bacterial suspension with a concentration of 1×10 7 ~2×10 7 cfu / mL.

[0059] Add 5 mL of the sample and 100 μL of the bacterial suspension to a sterile test tube and mix. For the control group, mix 5 mL of phosphate buffer solution and 100 μL of the bacterial suspension. After uniform mixing, start timing and let it act for 2 min and 4 min. Take 0.1 mL of the mixed sample solution and inoculate it into a sterile petri dish, then pour in the melted agar medium cooled to 45 °C and mix evenly. After solidification, place each inoculated plate in an incubator at 37 °C. Among them, Staphylococcus aureus is cultured at 37 °C for 48 h, and Candida albicans is cultured at 37 °C for 72 h. Conduct viable bacterial colony counting and calculate the antibacterial rate against Candida albicans and Staphylococcus aureus.

[0060] Antibacterial rate (%) = (number of bacteria in the control group - number of bacteria in the sample group) / number of bacteria in the control group × 100%

[0061] The results are shown in Table 1-2 below:

[0062] Table 1 Antibacterial rate against Candida albicans

[0063] Group 2 - minute Bacteriostatic Rate (%) 4 - minute Bacteriostatic Rate (%) Example 1 98.9 100 Example 2 93.4 100 Example 3 95.3 100 Comparative Example 1 81.1 91.4 Comparative Example 2 86.8 93.9 Comparative Example 3 85.7 92.3 Comparative Example 4 89.8 96.7 Comparative Example 5 87.4 95.9

[0064] Table 2 Antibacterial rate against Staphylococcus aureus

[0065] Group 2 - minute Bacteriostatic Rate (%) 4 - minute Bacteriostatic Rate (%) Example 1 99.3 100 Example 2 97.3 100 Example 3 98.4 100 Comparative Example 1 83.1 93.4 Comparative Example 2 87.5 95.9 Comparative Example 3 89.8 95.3 Comparative Example 4 90.8 97.7 Comparative Example 5 90.2 97.3

[0066] The above results show that the traditional Chinese medicine compound liquids in Examples 1 to 3 have good antibacterial effects against Candida albicans and Staphylococcus aureus, and the onset is rapid. Compared with Example 1, the antibacterial effects of Comparative Examples 1 to 5 have decreased.

[0067] Test Example 2

[0068] Collect patients with postpartum vaginal relaxation, randomly divide them into groups, with 20 cases in each group, including Research Groups 1 to 8 (Examples 1 to 3, Comparative Examples 1 to 5). There were no statistically significant differences in the ages and degrees of vaginal relaxation among the patients in each group (P>0.05), and they were comparable.

[0069] Usage method: After cleaning the hands and private parts, use the syringe. Each time, use 3 mL. Lift the hips and take out the syringe 30 - 40 min after injecting the traditional Chinese medicine compound liquid.

[0070] Compare the vaginal health indices of each group of patients before and after medication: It mainly includes four indicators: vaginal mucosa elasticity, secretion type and viscosity, vaginal mucosa epithelium, and vaginal moisture. Each indicator is scored from 1 to 5 points. The higher the score, the higher the vaginal health index. Calculate the improvement rate of the vaginal health index before and after medication.

[0071] Vaginal health index improvement rate = (Vaginal health index after medication - Vaginal health index before medication) / Vaginal health index before medication * 100%

[0072] Compare the degree of vaginal relaxation of each group of patients before and after medication: When the transverse diameter of the vagina can accommodate 2 - 3 transverse fingers side by side, it is mild relaxation, counted as 1 point; when the transverse diameter of the vagina can accommodate 3 - 4 transverse fingers side by side, it is moderate relaxation, counted as 2 points; when the transverse diameter of the vagina can accommodate more than 4 fingers, it is severe relaxation, counted as 3 points; when the transverse diameter of the vagina can accommodate less than 2 transverse fingers, it is normal, counted as 0 points. The higher the score, the more severe the degree of vaginal relaxation. Calculate the improvement rate of vaginal relaxation before and after medication.

[0073] Vaginal relaxation improvement rate = (Vaginal relaxation degree score before medication - Vaginal relaxation degree score after medication) / Vaginal health index before medication * 100%

[0074] Table 3 Comparison of vaginal improvement

[0075] Group Rate of Improvement in Vaginal Health Index (%) Rate of Improvement in Vaginal Relaxation (%) Example 1 363 98.3 Example 2 332 96.6 Example 3 331 94.8 Comparative Example 1 235 74.6 Comparative Example 2 282 86.2 Comparative Example 3 276 82.5 Comparative Example 4 318 91.4 Comparative Example 5 311 89.8

[0076] The results show that compared with the control group, the traditional Chinese medicine composite solution of Examples 1 - 3 significantly improves the vaginal health index and simultaneously significantly improves the degree of vaginal relaxation. Compared with Example 1, the improvement effects of Comparative Examples 1 - 5 decline.

[0077] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the principles of the present invention shall be included within the protection scope of the present invention.

Claims

1. A method for preparing a Chinese medicine compound having antibacterial and elastic properties and promoting vaginal repair, characterized in that: The following steps are involved: (1) extracting lithospermum officinale and saffron with supercritical CO2 to obtain lithospermum officinale-saffron extract; (2) crushing chrysanthemum and vitex agnus-castus after vacuum drying; extracting chrysanthemum and vitex agnus-castus powder by dynamic countercurrent, filtering, and collecting the filtrate; crushing pollen typhae, adding pollen typhae powder to the filtrate, performing ultrasonic-assisted extraction, filtering, and collecting the composite extract; (3) soaking Phellodendron amurense, Sophora flavescens, and Angelica sinensis in rice wine; mixing the Phellodendron amurense, Sophora flavescens, and Angelica sinensis soaked in rice wine with Herba Tripterygii, adding purified water, inoculating a composite bacterial strain consisting of Lactobacillus plantarum and Bacillus subtilis subsp. subtilis, fermenting, filtering, and obtaining a fermentation liquid; after pasteurization, mixing the fermentation liquid with an extract of Lithospermum officinale-Puhuang-Saffron and a composite extract to obtain a traditional Chinese medicine complex.

2. The Chinese medicine complex with antibacterial and elastic properties and promoting vaginal repair according to claim 1, characterized in that: Step (1), supercritical CO2 extraction temperature is 40-45°C, pressure is 20-30MPa, and extraction time is 2-6h.

3. The Chinese medicine complex with antibacterial and elastic properties and promoting vaginal repair according to claim 1 or 3, characterized in that: Step (2), the dynamic countercurrent extraction temperature is 60-70°C, the extraction is circulated 2-4 times, and the extraction solvent is 25-35% v / v ethanol solution.

4. The Chinese medicine complex with antibacterial and elastic properties and promoting vaginal repair according to claim 1, characterized in that: Step (2), ultrasonic assisted extraction frequency is 35-45kHz, power is 250-350W, and time is 15-25 minutes.

5. The Chinese medicinal compound with antibacterial and elastic properties and promoting vaginal repair according to claim 1, characterized in that: In step (3), the soaking time of rice wine is 20 to 40 minutes.

6. The Chinese medicinal compound with antibacterial and elastic properties and promoting vaginal repair according to claim 1 or 5, characterized in that: In step (3), the amount of purified water added is 7 to 9 times the total weight of the Chinese medicinal materials Phellodendron chinense, Sophora flavescens, Angelica sinensis and Three Immortal Sprouts, the inoculation amount of the composite strain is 2 to 4% of the total weight of the Chinese medicinal materials Phellodendron chinense, Sophora flavescens, Angelica sinensis and Three Immortal Sprouts, the composite strain is composed of Lactobacillus plantarum and Bacillus subtilis subsp. subtilis in a mass ratio of 1.5 to 2.5:0.8 to 1.2, the fermentation temperature is 30 to 37° C., and the fermentation time is 48 to 72 hours.

7. The method for preparing the Chinese medicine complex with antibacterial and elastic properties and promoting vaginal repair according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 15-20 parts of phellodendron, 10-15 parts of sophora flavescens, 8-12 parts of chrysanthemum, 8-12 parts of lithospermum officinale, 5-10 parts of pollen cattail, 10-15 parts of angelica sinensis, 3-5 parts of saffron, 6-10 parts of trichosanthes kirilowii and 5-8 parts of vitex agnus-flowered.

8. A Chinese medicine compound with antibacterial and elastic properties and promoting vaginal repair, characterized in that: Prepared by the preparation method according to any one of claims 1 to 7.

9. A Chinese medicine compound preparation with antibacterial and elastic properties and promoting vaginal repair, characterized in that: Prepared from the Chinese medicine composite according to claim 8.

10. The Chinese medicine complex preparation with antibacterial and elastic properties and promoting vaginal repair according to claim 9, characterized in that: The preparation method of the preparation comprises the following steps: adding chitosan quaternary ammonium salt and sodium hyaluronate to the traditional Chinese medicine complex, wherein the amount of chitosan quaternary ammonium salt added is 0.08% to 0.12% of the weight of the traditional Chinese medicine complex, and the amount of sodium hyaluronate added is 0.8% to 1.2% of the weight of the traditional Chinese medicine complex; filtering to obtain the target traditional Chinese medicine complex preparation.