Agricultural microbial fertilizer

Abstract

The invention relates to the technical field of plant regulators, in particular to an agricultural microbial fertilizer. The agricultural microbial fertilizer provided by the invention comprises a silicon-containing composition, a composite activator, a stabilizer and a complex microbial inoculant. The agricultural microbial fertilizer has a good fruit coloring effect.

Description

Technical Field

[0001] The present invention relates to the technical field of plant regulators, and particularly relates to an agricultural microbial fertilizer. Background Art

[0002] Plant regulators, also known as Plant Growth Regulators (abbreviated as PGR), are a class of chemical substances used to regulate the growth and development of plants. These substances can be synthetic or natural plant hormones extracted from organisms. They have similar chemical properties and physiological effects to plant hormones and can affect multiple growth and development processes of plants.

[0003] The quality of agricultural products has a great impact on farmers' income. The color of fruits is an important indicator for evaluating the appearance quality of fruits, which is generally valued by consumers and directly affects the quality and price. In recent years, promoting fruit coloring has been widely emphasized. The current methods mainly include: improving the lighting conditions of the tree body, controlling nitrogen fertilizer, increasing the application of phosphorus and potassium fertilizers, bagging, leaf removal, fruit rotation, laying reflective films, etc. Thiocyanate, thiosulfate, B9, 6-BA, abscisic acid, GA3, uniconazole, etc. can promote fruit coloring. However, improper use methods not only fail to achieve the effect but may also cause serious foliar phytotoxicity. So far, although many researchers have conducted a lot of experiments, very few plant growth regulators have been truly put into use. Either the coloring effect is not obvious or it is easy to cause some side effects.

[0004] Therefore, it is particularly important to develop a plant regulator with an obvious coloring effect. Summary of the Invention

[0005] In view of this, the purpose of the present invention is to provide an agricultural microbial fertilizer with an obvious coloring effect.

[0006] To achieve the above purpose, the present invention provides the following technical solutions:

[0007] In the first aspect, an agricultural microbial fertilizer is provided, and the agricultural microbial fertilizer includes a silicon-containing composition, a composite activator, a stabilizer, and a composite bacterial agent.

[0008] Preferably, by mass percentage, the agricultural microbial fertilizer includes 10-20% of the silicon-containing composition, 3-5% of the composite activator, 65-80% of the stabilizer, and 0.5-1% of the composite bacterial agent.

[0009] Preferably, the agricultural microbial fertilizer further includes plant exosomes, and the mass ratio of the plant exosomes to the agricultural microbial fertilizer is 0.3-0.6:1.

[0010] In the present invention, the plant exosomes are determined according to the types of experimental objects of agricultural microbial fertilizers. For example, when the experimental object of the agricultural microbial fertilizer is grapes, the plant exosomes are grape exosomes; when the experimental object of the agricultural microbial fertilizer is tomatoes, the plant exosomes are tomato exosomes.

[0011] Preferably, the preparation method of the silicon-containing composition comprises the following steps:

[0012] Step S1. Slowly drop hydrochloric acid into potassium silicate for reaction to obtain a potassium silicate acid solution;

[0013] Step S2. Slowly drop acetic acid into sodium silicate for reaction to obtain a sodium silicate acid solution;

[0014] Step S3. Mix the potassium silicate acid solution and the sodium silicate acid solution evenly according to a mass ratio of 0.5:1.5 to obtain the silicon-containing composition.

[0015] Preferably,

[0016] The composite activator includes a first activator, a second activator and a third activator, and the mass ratio of the first activator, the second activator and the third activator is 0.5:1:0.5;

[0017] The first activator is a compound of a zinc salt and sodium molybdate;

[0018] The second activator is a compound of a manganese salt and sodium molybdate;

[0019] The third activator is a compound of a copper salt and ammonium molybdate.

[0020] Preferably,

[0021] The preparation method of the first activator is as follows:

[0022] Dissolve the zinc salt in polyacrylic acid to obtain a first solution;

[0023] Dissolve sodium molybdate in ammonia water to obtain a second solution;

[0024] Mix the first solution and the second solution evenly to obtain the first activator; wherein, the mixing temperature is 25-35°C and the mixing time is 2-3 h;

[0025] The preparation method of the second activator is as follows:

[0026] Dissolve the manganese salt in ethanol to obtain a third solution;

[0027] Dissolve sodium molybdate in water to obtain a fourth solution;

[0028] Mix the third dissolution solution and the fourth dissolution solution evenly to obtain the second activator; wherein, the mixing temperature is 30 - 40 °C and the mixing time is 2.5 - 3.5 h;

[0029] The preparation method of the third activator is as follows:

[0030] Dissolve copper salt in ethanol to obtain the fifth dissolution solution;

[0031] Dissolve ammonium molybdate in hydrobromic acid to obtain the sixth dissolution solution;

[0032] Mix the fifth dissolution solution and the sixth dissolution solution evenly to obtain the third activator; wherein, the mixing temperature is 40 - 50 °C and the mixing time is 3 - 4 h.

[0033] Preferably,

[0034] The stabilizer is amyl alcohol;

[0035] The stabilizer is composed of n - amyl alcohol, iso - amyl alcohol and 2 - methyl - 1 - butanol;

[0036] The mass ratio of the n - amyl alcohol, the iso - amyl alcohol and the 2 - methyl - 1 - butanol is 1:1:1.

[0037] Preferably,

[0038] The compound microbial agent is compounded from the fermentation broth of Trichoderma and the fermentation broth of Streptomyces according to a mass ratio of 1 - 3:1 - 5;

[0039] The Trichoderma is Trichoderma longibrachiatum and the Streptomyces is Streptomyces griseus.

[0040] In the second aspect, a preparation method of an agricultural microbial fertilizer is provided. The preparation method includes the following steps:

[0041] 1) Divide the stabilizer evenly into the first stabilizer, the second stabilizer and the third stabilizer;

[0042] 2) Add the compound activator to the first stabilizer to prepare a compound activator stable solution;

[0043] 3) Add the compound microbial agent to the second stabilizer to prepare a compound microbial agent stable solution;

[0044] 4) Add the silicon - containing composition to the third stabilizer to prepare a silicon - containing composition stable solution;

[0045] 5) Mix the compound activator stable solution, the compound microbial agent stable solution and the silicon - containing composition stable solution evenly to obtain the agricultural microbial fertilizer; wherein, the mixing temperature is 40 - 60 °C and the mixing time is 5 - 6 h.

[0046] Thirdly, the present invention provides the application of the agricultural microbial fertilizer described in the present invention in the preparation of plant regulators.

[0047] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0048] The agricultural microbial fertilizer of the present invention comprising a silicon-containing composition, a composite activator, a stabilizer and a composite bacterial agent can significantly improve the coloring effect of fruits. The composite activator including a first activator, a second activator and a third activator used in the agricultural microbial fertilizer of the present invention can make the fertilizer effect of the agricultural microbial fertilizer better, that is, the coloring effect is better; by optimizing the mass ratio of the first activator, the second activator and the third activator, the coloring effect can be further improved.

[0049] Moreover, the present invention proves that adding a composite bacterial agent composed of the fermentation broth of Trichoderma longibrachiatum and the fermentation broth of Streptomyces griseus to the agricultural microbial fertilizer can further improve the coloring effect of fruits. The present invention also proves that adding plant exosomes to the agricultural microbial fertilizer and cooperating with the composite bacterial agent of the present invention can further improve the coloring effect of fruits.

[0050] Regarding further improving the coloring effect of fruits, the applicant speculates that plant exosomes may affect the hormone balance or signal transduction in plants in some way, and then regulate the synthesis pathway of anthocyanins (for example, plant exosomes may contain growth factors or signal molecules that promote anthocyanin synthesis); or plant exosomes may be rich in minerals, vitamins and other nutrients beneficial to fruit coloring, and these components can be directly absorbed and utilized by fruits to promote the synthesis and accumulation of pigments. Specific Embodiments

[0051] The following will specifically describe the present invention in combination with specific embodiments and examples, and the advantages and various effects of the present invention will be presented more clearly therefrom. Those skilled in the art should understand that these specific embodiments and examples are used to illustrate the present invention, rather than to limit the present invention.

[0052] In the embodiments of the present invention, the effective viable count of Trichoderma longibrachiatum is not less than 100 million CFU / mL, and the effective viable count of Streptomyces griseus is not less than 100 million CFU / mL.

[0053] In the embodiments of the present invention, the composite bacterial agent is composed of the fermentation broth of Trichoderma longibrachiatum and the fermentation broth of Streptomyces griseus in a mass ratio of 1-3:1-5, and their compatibility is verified by an antagonistic test (the diameter of the inhibition zone ≤ 2 mm).

[0054] In the embodiments of the present invention, Trichoderma longibrachiatum and Streptomyces griseus were both purchased from the General Microbiology Center of the China Committee for Culture Collection of Microorganisms. Trichoderma longibrachiatum refers to Trichoderma longibrachiatum with the preservation number of CGMCC No. 3.13106, and Streptomyces griseus refers to Streptomyces griseus with the preservation number of CGMCC No. 4.5718.

[0055] The preparation method of the fermentation broth of Trichoderma longibrachiatum is as follows:

[0056] 1) Inoculate the spores of Trichoderma longibrachiatum onto a PDA medium (Potato Dextrose Agar) plate and culture at 28°C for 3 - 5 days to form single colonies;

[0057] 2) Pick single colonies and inoculate them into a liquid seed medium (formula: glucose 20 g / L, peptone 5 g / L, KH2PO4 2 g / L, MgSO4·7H2O 1 g / L), and culture on a shaker at 28°C and 180 rpm for 48 h to obtain a seed solution;

[0058] 3) Transfer the seed solution to a fermentation medium (formula: corn flour 30 g / L, soybean meal powder 10 g / L, (NH4)2SO4 3 g / L, KH2PO4 1 g / L, CaCO3 2 g / L) at an inoculation amount of 5%. Fermentation conditions: temperature 28°C, aeration rate 1.0 vvm (volume ratio), stirring speed 200 rpm, and ferment for 72 h; monitor the pH (maintained at 5.0 - 6.0) and the cell biomass (OD 600 ≥4.0) during the fermentation process;

[0059] 4) After the fermentation is completed, centrifuge (4000 rpm, 15 min) to remove the cell residues, and collect the supernatant, which is the fermentation broth of Trichoderma longibrachiatum.

[0060] The preparation method of the fermentation broth of Streptomyces griseus is as follows:

[0061] 1) Inoculate the spores of Streptomyces griseus onto a Czapek-Dox medium (soluble starch 20 g / L, KNO3 1 g / L, K2HPO4 0.5 g / L, MgSO4·7H2O 0.5 g / L, FeSO4 0.01 g / L), and culture at 28°C for 5 - 7 days to form spore chains;

[0062] 2) Scrape the spores and inoculate them into a liquid seed medium (formula: glucose 10 g / L, yeast extract 5 g / L, malt extract 5 g / L), and culture on a shaker at 28°C and 200 rpm for 36 h to obtain a seed solution;

[0063] 3) Transfer the seed liquid to the fermentation medium at an inoculation amount of 8% (formula: soluble starch 40 g / L, soybean cake powder 15 g / L, NaCl 3 g / L, CaCO3 4 g / L); fermentation conditions: temperature 28 °C, aeration rate 1.5 vvm, stirring speed 250 rpm, ferment for 96 h. Monitor the dissolved oxygen content (DO ≥ 30%) and secondary metabolites (streptomycin titer ≥ 8000 U / mL);

[0064] 4) After fermentation, remove the mycelium by plate and frame filtration, and concentrate the filtrate by ultrafiltration (cut-off molecular weight 10 kDa) to obtain the fermentation broth of Streptomyces griseus.

[0065] In the examples of the present invention, the preparation method of plant exosomes is as follows:

[0066] ① Add sterilized PBS to fresh plants and use a crusher to crush until a uniform paste is formed; centrifuge the crushed paste at 5 °C, (3000 - 3500) × g for 30 - 50 min to remove larger residues and impurities, and take the solution in the middle layer;

[0067] ② Centrifuge the solution obtained in step ① at 5 °C, (5000 - 10000) × g for 40 - 50 min, and take the supernatant;

[0068] ③ Centrifuge the supernatant obtained in step ② at 5 °C, (100000 - 120000) × g for 55 - 65 min, then resuspend the precipitate with sterile PBS at 5 °C, centrifuge at (120000 - 150000) × g for 65 - 70 min, and resuspend the precipitate with sterile PBS again to obtain plant exosomes.

[0069] Example 1

[0070] This example provides an agricultural microbial fertilizer. By mass percentage, the agricultural microbial fertilizer includes 15% silicon-containing composition, 4% composite activator, 70% stabilizer, and 1% composite bacterial agent;

[0071] The preparation method of the silicon-containing composition includes the following steps:

[0072] Step S1. Slowly drip hydrochloric acid into potassium silicate for reaction to obtain potassium silicate acid solution;

[0073] Step S2. Slowly drip acetic acid into sodium silicate for reaction to obtain sodium silicate acid solution;

[0074] Step S3. Mix the potassium silicate acid solution and the sodium silicate acid solution evenly according to a mass ratio of 0.5:1.5 to obtain the silicon-containing composition;

[0075] The composite activator includes a first activator, a second activator, and a third activator, and the mass ratio of the first activator, the second activator, and the third activator is 0.5:1:0.5;

[0076] The first activator is composed of a zinc salt and sodium molybdate in combination;

[0077] The preparation method of the first activator is as follows:

[0078] Dissolve the zinc salt in polyacrylic acid to obtain a first solution;

[0079] Dissolve sodium molybdate in ammonia water to obtain a second solution;

[0080] Mix the first solution and the second solution evenly to obtain the first activator; wherein, the mixing temperature is 30°C and the mixing time is 3 h;

[0081] The second activator is composed of a manganese salt and sodium molybdate in combination;

[0082] The preparation method of the second activator is as follows:

[0083] Dissolve the manganese salt in ethanol to obtain a third solution;

[0084] Dissolve sodium molybdate in water to obtain a fourth solution;

[0085] Mix the third solution and the fourth solution evenly to obtain the second activator; wherein, the mixing temperature is 35°C and the mixing time is 3 h;

[0086] The third activator is composed of a copper salt and ammonium molybdate in combination;

[0087] The preparation method of the third activator is as follows:

[0088] Dissolve the copper salt in ethanol to obtain a fifth solution;

[0089] Dissolve ammonium molybdate in hydrobromic acid to obtain a sixth solution;

[0090] Mix the fifth solution and the sixth solution evenly to obtain the third activator; wherein, the mixing temperature is 45°C and the mixing time is 4 h;

[0091] The stabilizer is prepared from n-pentanol, iso-pentanol, and 2-methyl-1-butanol according to a mass ratio of 1:1:1;

[0092] The composite bacterium agent is composed of the fermentation broth of Trichoderma longibrachiatum and the fermentation broth of Streptomyces griseus according to a mass ratio of 2:3 in combination.

[0093] Example 2

[0094] This embodiment provides an agricultural microbial fertilizer. By mass percentage, the agricultural microbial fertilizer includes 10% silicon-containing composition, 3% composite activator, 65% stabilizer, and 0.5% composite bacterial agent;

[0095] The agricultural microbial fertilizer further includes plant exosomes, and the mass ratio of plant exosomes to the agricultural microbial fertilizer is 0.3:1;

[0096] The preparation method of the silicon-containing composition includes the following steps:

[0097] Step S1. Slowly drip hydrochloric acid into potassium silicate for reaction to obtain potassium silicate acid solution;

[0098] Step S2. Slowly drip acetic acid into sodium silicate for reaction to obtain sodium silicate acid solution;

[0099] Step S3. Mix the potassium silicate acid solution and the sodium silicate acid solution evenly according to the mass ratio of 0.5:1.5 to obtain the silicon-containing composition;

[0100] The composite activator includes a first activator, a second activator, and a third activator, and the mass ratio of the first activator, the second activator, and the third activator is 0.5:1:0.5;

[0101] The first activator is a compound of zinc salt and sodium molybdate;

[0102] The preparation method of the first activator is as follows:

[0103] Dissolve the zinc salt in polyacrylic acid to obtain a first solution;

[0104] Dissolve sodium molybdate in ammonia water to obtain a second solution;

[0105] Mix the first solution and the second solution evenly to obtain the first activator; among them, the mixing temperature is 25°C and the mixing time is 2 h;

[0106] The second activator is a compound of manganese salt and sodium molybdate;

[0107] The preparation method of the second activator is as follows:

[0108] Dissolve the manganese salt in ethanol to obtain a third solution;

[0109] Dissolve sodium molybdate in water to obtain a fourth solution;

[0110] Mix the third solution and the fourth solution evenly to obtain the second activator; among them, the mixing temperature is 30°C and the mixing time is 2.5 h;

[0111] The third activator is a compound of copper salt and ammonium molybdate;

[0112] The preparation method of the third activator is as follows:

[0113] Dissolve the copper salt in ethanol to obtain the fifth solution;

[0114] Dissolve ammonium molybdate in hydrobromic acid to obtain the sixth solution;

[0115] Mix the fifth solution and the sixth solution evenly to obtain the third activator; among them, the mixing temperature is 40 °C and the mixing time is 3 h;

[0116] The stabilizer is prepared from n-pentanol, iso-pentanol and 2-methyl-1-butanol according to a mass ratio of 1:1:1;

[0117] The compound microbial agent is compounded from the fermentation broth of Trichoderma longibrachiatum and the fermentation broth of Streptomyces griseus according to a mass ratio of 1:1.

[0118] Example 3

[0119] This example provides an agricultural microbial fertilizer. By mass percentage, the agricultural microbial fertilizer includes 20% silicon-containing composition, 5% compound activator, 80% stabilizer and 1% compound microbial agent;

[0120] The agricultural microbial fertilizer further includes plant exosomes, and the mass ratio of plant exosomes to the agricultural microbial fertilizer is 0.6:1;

[0121] The preparation method of the silicon-containing composition includes the following steps:

[0122] Step S1. Slowly drop hydrochloric acid into potassium silicate for reaction to obtain potassium silicate acid solution;

[0123] Step S2. Slowly drop acetic acid into sodium silicate for reaction to obtain sodium silicate acid solution;

[0124] Step S3. Mix the potassium silicate acid solution and the sodium silicate acid solution evenly according to a mass ratio of 0.5:1.5 to obtain the silicon-containing composition;

[0125] The compound activator includes a first activator, a second activator and a third activator, and the mass ratio of the first activator, the second activator and the third activator is 0.5:1:0.5;

[0126] The first activator is a compound of zinc salt and sodium molybdate;

[0127] The preparation method of the first activator is as follows:

[0128] Dissolve the zinc salt in polyacrylic acid to obtain the first solution;

[0129] Dissolve sodium molybdate in ammonia water to obtain the second solution;

[0130] Mix the first dissolution solution and the second dissolution solution evenly to obtain the first activator; wherein, the mixing temperature is 35 °C and the mixing time is 3 h;

[0131] The second activator is composed of a manganese salt and sodium molybdate in combination;

[0132] The preparation method of the second activator is as follows:

[0133] Dissolve the manganese salt in ethanol to obtain the third dissolution solution;

[0134] Dissolve sodium molybdate in water to obtain the fourth dissolution solution;

[0135] Mix the third dissolution solution and the fourth dissolution solution evenly to obtain the second activator; wherein, the mixing temperature is 40 °C and the mixing time is 3.5 h;

[0136] The third activator is composed of a copper salt and ammonium molybdate in combination;

[0137] The preparation method of the third activator is as follows:

[0138] Dissolve the copper salt in ethanol to obtain the fifth dissolution solution;

[0139] Dissolve ammonium molybdate in hydrobromic acid to obtain the sixth dissolution solution;

[0140] Mix the fifth dissolution solution and the sixth dissolution solution evenly to obtain the third activator; wherein, the mixing temperature is 50 °C and the mixing time is 4 h;

[0141] The stabilizer is prepared from n-pentanol, iso-pentanol and 2-methyl-1-butanol in a mass ratio of 1:1:1;

[0142] The compound microbial agent is composed of the fermentation broth of Trichoderma longibrachiatum and the fermentation broth of Streptomyces griseus in a mass ratio of 3:5.

[0143] Example 4

[0144] This example provides an agricultural microbial fertilizer. By mass percentage, the agricultural microbial fertilizer includes 15% silicon-containing composition, 4% compound activator, 70% stabilizer and 1% compound microbial agent;

[0145] The agricultural microbial fertilizer further includes plant exosomes, and the mass ratio of the plant exosomes to the agricultural microbial fertilizer is 0.5:1;

[0146] The preparation method of the silicon-containing composition includes the following steps:

[0147] Step S1. Slowly drop hydrochloric acid into potassium silicate for reaction to obtain potassium silicate acid solution;

[0148] Step S2. Slowly drop acetic acid into sodium silicate for reaction to obtain sodium silicate acid solution;

[0149] Step S3. Mix the potassium silicate acid solution and the sodium silicate acid solution evenly according to a mass ratio of 0.5:1.5 to obtain a silicon-containing composition;

[0150] The composite activator includes a first activator, a second activator, and a third activator, and the mass ratio of the first activator, the second activator, and the third activator is 0.5:1:0.5;

[0151] The first activator is a compound of a zinc salt and sodium molybdate;

[0152] The preparation method of the first activator is as follows:

[0153] Dissolve the zinc salt in polyacrylic acid to obtain a first solution;

[0154] Dissolve sodium molybdate in ammonia water to obtain a second solution;

[0155] Mix the first solution and the second solution evenly to obtain the first activator; wherein, the mixing temperature is 30 °C and the mixing time is 3 h;

[0156] The second activator is a compound of a manganese salt and sodium molybdate;

[0157] The preparation method of the second activator is as follows:

[0158] Dissolve the manganese salt in ethanol to obtain a third solution;

[0159] Dissolve sodium molybdate in water to obtain a fourth solution;

[0160] Mix the third solution and the fourth solution evenly to obtain the second activator; wherein, the mixing temperature is 35 °C and the mixing time is 3 h;

[0161] The third activator is a compound of a copper salt and ammonium molybdate;

[0162] The preparation method of the third activator is as follows:

[0163] Dissolve the copper salt in ethanol to obtain a fifth solution;

[0164] Dissolve ammonium molybdate in hydrobromic acid to obtain a sixth solution;

[0165] Mix the fifth solution and the sixth solution evenly to obtain the third activator; wherein, the mixing temperature is 45 °C and the mixing time is 4 h;

[0166] The stabilizer is prepared from n-pentanol, iso-pentanol, and 2-methyl-1-butanol according to a mass ratio of 1:1:1;

[0167] The composite microbial agent is composed of the fermentation broth of Trichoderma longibrachiatum and the fermentation broth of Streptomyces griseus, compounded according to a mass ratio of 2:3.

[0168] Example 5

[0169] This example provides a preparation method of an agricultural microbial fertilizer, and the preparation method includes the following steps:

[0170] 1) Divide the stabilizer into a first stabilizer, a second stabilizer, and a third stabilizer evenly;

[0171] 2) Add the composite activator to the first stabilizer to prepare a composite activator stable solution;

[0172] 3) Add the composite microbial agent to the second stabilizer to prepare a composite microbial agent stable solution;

[0173] 4) Add the silicon-containing composition to the third stabilizer to prepare a silicon-containing composition stable solution;

[0174] 5) Mix the composite activator stable solution, the composite microbial agent stable solution, and the silicon-containing composition stable solution evenly to obtain an agricultural microbial fertilizer; wherein, the mixing temperature is 40-60 °C, and the mixing time is 5-6 h.

[0175] 6) Mix the plant exosomes and the agricultural microbial fertilizer evenly according to a mass ratio of 0.3-0.6:1.

[0176] Comparative Example 1

[0177] The difference between this comparative example and Example 4 is that: the mass percentage of the silicon-containing composition in the agricultural microbial fertilizer is 8%;

[0178] Other conditions are the same as those in Example 4.

[0179] Comparative Example 2

[0180] The difference between this comparative example and Example 4 is that: the silicon-containing composition is not added to the agricultural microbial fertilizer;

[0181] Other conditions are the same as those in Example 4.

[0182] Comparative Example 3

[0183] The difference between this comparative example and Example 4 is that: n-pentanol in the stabilizer is replaced by 3-methyl-2-butanol;

[0184] Other conditions are the same as those in Example 4.

[0185] Effect verification

[0186] Experiment 1 Verification of grape coloring effect

[0187] Experimental object: Grape

[0188] Spraying method: Dilute the agricultural microbial fertilizers of Examples 1 - 4 and Comparative Examples 1 - 3 prepared according to the preparation method of Example 5 by 1000 times, and spray three times in total. The first spraying is carried out 15 days after the flower withering, the second spraying is carried out before bagging, and the third spraying is carried out 30 days before harvesting.

[0189] Among them, the grape coloring grading is evaluated according to the following criteria:

[0190] Grade 0: All green;

[0191] Grade 1: Slight coloring, and the pink fruit grains account for 1 - 10% of the whole spike;

[0192] Grade 2: The colored fruit grains (pink) account for 10 - 50% of the fruit spike;

[0193] Grade 3: The colored fruit grains account for 50 - 100% of the whole spike;

[0194] Grade 4: All colored. There are some black fruit grains, and the black fruit grains account for 1 - 50% of the whole spike;

[0195] Grade 5: All colored, with more brown fruit grains, accounting for 50 - 100% of the whole spike.

[0196] Experimental results: The coloring situation of the grapes 30 days after spraying is shown in Table 1.

[0197] Table 1 Grape coloring effect

[0198]

[0199]

[0200] Similarly, in order to further illustrate the beneficial effects of the present invention, the following comparative examples are provided:

[0201] Provide Comparative Example 1.1. Compared with Example 4, the differences of this Comparative Example 1.1 are as follows:

[0202] The mass percentage of the silicon-containing composition in the agricultural microbial fertilizer is 22%;

[0203] Or the mass percentage of the composite activator in the agricultural microbial fertilizer is 2%;

[0204] Or the mass percentage of the composite activator in the agricultural microbial fertilizer is 6%;

[0205] Or the mass percentage of the stabilizer in the agricultural microbial fertilizer is 62%;

[0206] Or the mass percentage of the stabilizer in the agricultural microbial fertilizer is 82%;

[0207] or the mass percentage of the compound microbial agent in the agricultural microbial fertilizer is 0.3%;

[0208] or the mass percentage of the compound microbial agent in the agricultural microbial fertilizer is 1.2%;

[0209] or the mass ratio of the plant exosomes to the agricultural microbial fertilizer is 0.2:1;

[0210] or the mass ratio of the plant exosomes to the agricultural microbial fertilizer is 0.7:1;

[0211] or the mass ratio of the plant exosomes to the agricultural microbial fertilizer is 0.5:1.2;

[0212] or the mass ratio of the plant exosomes to the agricultural microbial fertilizer is 0.5:0.8;

[0213] or the mass ratio of n-pentanol, iso-pentanol and 2-methyl-1-butanol is 0.8:1:1;

[0214] or the compound microbial agent is compounded by the fermentation broth of Trichoderma and the fermentation broth of Streptomyces in a mass ratio of 2:6;

[0215] or the compound microbial agent is compounded by the fermentation broth of Trichoderma and the fermentation broth of Streptomyces in a mass ratio of 0.5:3;

[0216] Other conditions are the same as those in Example 4.

[0217] The agricultural microbial fertilizer is prepared by combining the preparation method of the present invention and tested by the same method as in Experiment 1. Observe the grape coloring effect of the agricultural microbial fertilizer prepared in Comparative Example 1.1. The results are similar to those in Comparative Example 1 above.

[0218] Similarly, in order to further illustrate the beneficial effects of the present invention, the following comparative examples are provided:

[0219] Provide Comparative Example 2.1. Compared with Example 4, the difference in this Comparative Example 2.1 is that:

[0220] The compound activator is not added to the agricultural microbial fertilizer;

[0221] or the stabilizer is not added to the agricultural microbial fertilizer;

[0222] or the compound microbial agent is not added to the agricultural microbial fertilizer;

[0223] or the fermentation broth of Trichoderma is not added to the compound microbial agent;

[0224] or the fermentation broth of Streptomyces is not added to the compound microbial agent

[0225] or n-pentanol is not added to the stabilizer;

[0226] Other conditions are the same as those in Example 4.

[0227] Prepared into an agricultural microbial fertilizer by combining with the preparation method of the present invention, and tested according to the same method as the test method in Experiment 1. Observe the grape coloring effect of the agricultural microbial fertilizer prepared in Comparative Example 2.1. The results are similar to those in Comparative Example 2 above.

[0228] Similarly, to further illustrate the beneficial effects of the present invention, the following comparative examples are provided:

[0229] Provide Comparative Example 3.1. Compared with Example 4, the difference in this Comparative Example 3.1 is that:

[0230] Replace the fermentation broth of Trichoderma in the compound microbial agent with the fermentation broth of Aspergillus flavus;

[0231] Replace the fermentation broth of Streptomyces in the compound microbial agent with the fermentation broth of Aspergillus flavus;

[0232] Other conditions are the same as those in Example 4.

[0233] Prepared into an agricultural microbial fertilizer by combining with the preparation method of the present invention, and tested according to the same method as the test method in Experiment 1. Observe the grape coloring effect of the agricultural microbial fertilizer prepared in Comparative Example 3.1. The results are similar to those in Comparative Example 3 above.

[0234] As can be seen from Table 1, compared with the agricultural microbial fertilizer without using the formula system of the present invention, the agricultural microbial fertilizer of the present invention can significantly promote the coloring of grapes, and the coloring speed is fast and the coloring effect is obvious; compared with the water group, the agricultural microbial fertilizer of the present invention has an obvious improvement effect in promoting the coloring of grapes. Moreover, the agricultural microbial fertilizer including plant exosomes has the best effect in promoting the coloring of grapes.

[0235] Experiment 2 Verification of Tomato Coloring Effect

[0236] Experimental object: Tomato

[0237] Spraying method: Dilute the agricultural microbial fertilizers of Examples 1 - 4 and Comparative Examples 1 - 3 prepared according to the preparation method of Example 5 by 1000 times, and spray twice in total. Spray the first time 15 days after the flowers wither, and spray the second time 30 days before harvesting;

[0238] Among them, the tomato coloring grade is evaluated according to the following criteria:

[0239] Grade 0: All green;

[0240] Grade 1: Slight coloring, and the pink area accounts for 1 - 20% of the whole fruit;

[0241] Grade 2: The coloring area accounts for 20 - 40% of the whole fruit;

[0242] Level 3: The colored area accounts for 40 - 60% of the whole fruit;

[0243] Level 4: The colored area accounts for 60 - 80% of the whole fruit;

[0244] Level 5: The colored area accounts for 80 - 100% of the whole fruit;

[0245] Experimental results: The coloring situation of tomatoes 30 days after spraying is shown in Table 2.

[0246] Table 2 Coloring effect of tomatoes

[0247] Grouping Coloring situation Coloring level Example 1 94％ Level 5 Example 2 96％ Level 5 Example 3 98％ Level 5 Example 4 100％ Level 5 Comparative example 1 57％ Level 3 Comparative example 2 50％ Level 3 Comparative example 3 52％ Level 3 Clear water group 50％ Level 3

[0248] Similarly, in order to further illustrate the beneficial effects of the present invention, the following comparative examples are provided:

[0249] Provide Comparative Example 1.1. Compared with Example 4, the difference of this Comparative Example 1.1 is that:

[0250] The mass percentage of the silicon-containing composition in the agricultural microbial fertilizer is 22%;

[0251] Or the mass percentage of the composite activator in the agricultural microbial fertilizer is 2%;

[0252] Or the mass percentage of the composite activator in the agricultural microbial fertilizer is 6%;

[0253] Or the mass percentage of the stabilizer in the agricultural microbial fertilizer is 62%;

[0254] Or the mass percentage of the stabilizer in the agricultural microbial fertilizer is 82%;

[0255] Or the mass percentage of the composite microbial agent in the agricultural microbial fertilizer is 0.3%;

[0256] Or the mass percentage of the composite microbial agent in the agricultural microbial fertilizer is 1.2%;

[0257] Or the mass ratio of plant exosomes to the agricultural microbial fertilizer is 0.2:1;

[0258] Or the mass ratio of plant exosomes to the agricultural microbial fertilizer is 0.7:1;

[0259] Or the mass ratio of plant exosomes to the agricultural microbial fertilizer is 0.5:1.2;

[0260] Or the mass ratio of plant exosomes to the agricultural microbial fertilizer is 0.5:0.8;

[0261] Or the mass ratio of n-pentanol, iso-pentanol and 2-methyl-1-butanol is 0.8:1:1;

[0262] Or the compound microbial agent is compounded from the fermentation broth of Trichoderma and the fermentation broth of Streptomyces in a mass ratio of 2:6;

[0263] Or the compound microbial agent is compounded from the fermentation broth of Trichoderma and the fermentation broth of Streptomyces in a mass ratio of 0.5:3;

[0264] Other conditions are the same as those in Example 4.

[0265] Prepared into an agricultural microbial fertilizer by combining the preparation method of the present invention and tested by the same method as in Experiment 2. Observe the coloring effect of the tomatoes of the agricultural microbial fertilizer prepared in Comparative Example 1.1. The results are similar to those in Comparative Example 1 above.

[0266] Similarly, in order to further illustrate the beneficial effects of the present invention, the following comparative examples are provided:

[0267] Provide Comparative Example 2.1. Compared with Example 4, the difference in this Comparative Example 2.1 is that:

[0268] The compound activator is not added to the agricultural microbial fertilizer;

[0269] Or the stabilizer is not added to the agricultural microbial fertilizer;

[0270] Or the compound microbial agent is not added to the agricultural microbial fertilizer;

[0271] Or the fermentation broth of Trichoderma is not added to the compound microbial agent;

[0272] Or the fermentation broth of Streptomyces is not added to the compound microbial agent;

[0273] Or n-pentanol is not added to the stabilizer;

[0274] Other conditions are the same as those in Example 4.

[0275] Prepared into an agricultural microbial fertilizer by combining the preparation method of the present invention and tested by the same method as in Experiment 2. Observe the coloring effect of the tomatoes of the agricultural microbial fertilizer prepared in Comparative Example 2.1. The results are similar to those in Comparative Example 2 above.

[0276] Similarly, in order to further illustrate the beneficial effects of the present invention, the following comparative examples are provided:

[0277] Provide Comparative Example 3.1. Compared with Example 4, the difference in this Comparative Example 3.1 is that:

[0278] Replace the fermentation broth of Trichoderma in the compound microbial agent with the fermentation broth of Aspergillus flavus;

[0279] Replace the fermentation broth of Streptomyces in the compound microbial agent with the fermentation broth of Aspergillus flavus;

[0280] Other conditions are the same as those in Example 4.

[0281] The agricultural microbial fertilizer is prepared by combining with the preparation method of the present invention and tested by the same method as that in Experiment 2. Observe the coloring effect of the agricultural microbial fertilizer prepared in Comparative Example 3.1. The results are similar to those in Comparative Example 3 above.

[0282] As can be seen from Table 2, compared with the agricultural microbial fertilizer that does not use the formula system of the present invention, the agricultural microbial fertilizer of the present invention can significantly promote the coloring of tomatoes, with a fast coloring speed and obvious coloring effect; compared with the water group, the agricultural microbial fertilizer of the present invention has an obvious improvement effect on promoting the coloring of tomatoes. Moreover, the agricultural microbial fertilizer including plant exosomes has the best effect on promoting the coloring of tomatoes.

[0283] It should be understood that the present invention disclosed is not limited to the specific methods, schemes and substances described, as these can vary. It should also be understood that the terms used herein are only for the purpose of describing specific embodiments of the present invention and are not intended to limit the scope of the present invention, which is only limited by the appended claims.

[0284] Those skilled in the art will also recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. These equivalents are also included in the appended claims.

Claims

1. An agricultural microbial fertilizer, characterized in that: The agricultural microbial fertilizer comprises a silicon-containing composition, a composite activator, a stabilizer and a composite bacterial agent.

2. The agricultural microbial fertilizer according to claim 1, characterized in that: According to mass percentage, the agricultural microbial fertilizer includes 10-20% of a silicon-containing composition, 3-5% of a composite activator, 65-80% of a stabilizer and 0.5-1% of a composite bacterial agent.

3. The agricultural microbial fertilizer according to claim 1, characterized in that: The agricultural microbial fertilizer also includes plant exosomes, and the mass ratio of the plant exosomes to the agricultural microbial fertilizer is 0.3-0.6:

1.

4. The agricultural microbial fertilizer according to claim 1, characterized in that: The method for preparing the silicon-containing composition comprises the following steps: Step S1. slowly dripping hydrochloric acid into potassium silicate to react and obtain potassium silicate acid solution; Step S2. slowly dripping acetic acid into sodium silicate to react and obtain sodium silicate acid solution; Step S3. Mix the potassium silicate acid solution and the sodium silicate acid solution in a mass ratio of 0.5:1.5 to obtain the silicon-containing composition.

5. The agricultural microbial fertilizer according to claim 1, characterized in that: The composite activator comprises a first activator, a second activator and a third activator, and the mass ratio of the first activator, the second activator and the third activator is 0.5:1:0.5; The first activator is a compound of zinc salt and sodium molybdate; The second activator is a compound of manganese salt and sodium molybdate; The third activator is a compound of copper salt and ammonium molybdate.

6. The agricultural microbial fertilizer according to claim 5, characterized in that: The preparation method of the first activator is as follows: Dissolving zinc salt in polyacrylic acid to obtain a first solution; Dissolving sodium molybdate in aqueous ammonia to obtain a second solution; The first dissolving liquid and the second dissolving liquid are mixed evenly to obtain a first activator; wherein the mixing temperature is 25-35° C. and the mixing time is 2-3 hours; The preparation method of the second activator is as follows: dissolving the manganese salt in ethanol to obtain a third solution; Dissolving sodium molybdate in water to obtain a fourth solution; The third dissolving liquid and the fourth dissolving liquid are mixed evenly to obtain a second activator; wherein the mixing temperature is 30-40° C. and the mixing time is 2.5-3.5 hours; The preparation method of the third activator is as follows: Dissolving the copper salt in ethanol to obtain a fifth solution; dissolving ammonium molybdate in hydrobromic acid to obtain a sixth solution; The fifth dissolving liquid and the sixth dissolving liquid are mixed evenly to obtain a third activator; wherein the mixing temperature is 40-50° C. and the mixing time is 3-4 hours.

7. The agricultural microbial fertilizer according to claim 1, characterized in that: The stabilizer is amyl alcohol; The stabilizer consists of n-amyl alcohol, isoamyl alcohol and 2-methyl-1-butanol; The mass ratio of the n-pentanol, the isopentanol and the 2-methyl-1-butanol is 1:1:

1.

8. The agricultural microbial fertilizer according to claim 1, characterized in that: The composite bacterial agent is compounded by the fermentation broth of Trichoderma and the fermentation broth of Streptomyces in a mass ratio of 1-3:1-5; The Trichoderma is Trichoderma longibrachiatum, and the Streptomyces is Streptomyces griseus.

9. A method for preparing agricultural microbial fertilizer, characterized in that: The preparation method comprises the following steps: 1) The stabilizer is equally divided into a first stabilizer, a second stabilizer and a third stabilizer; 2) adding the composite activator to the first stabilizer to prepare a composite activator stabilized solution; 3) adding the composite bacterial agent to the second stabilizer to prepare a composite bacterial agent stabilized solution; 4) adding the silicon-containing composition to a third stabilizer to prepare a stable solution of the silicon-containing composition; 5) The composite activator stabilized solution, the composite bacterial agent stabilized solution and the silicon-containing composition stabilized solution are uniformly mixed to obtain agricultural microbial fertilizer; wherein the mixing temperature is 40-60° C. and the mixing time is 5-6 hours.

10. Use of the agricultural microbial fertilizer according to any one of claims 1 to 8 in the preparation of plant regulators.

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