A protein enzymatic hydrolysis reaction system and process

By introducing an adjustable homogenizing component and a piston column negative pressure effect into the protein enzymatic hydrolysis reaction system, the problems of fixed stirring range and uneven shear force distribution are solved, achieving more efficient enzymatic hydrolysis reaction and material mixing.

CN120158366BActive Publication Date: 2025-09-05FUJIAN YAMING FOOD
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Patent Information

Application Number
CN202510649240.2
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-05-20
Publication Date
2025-09-05
Estimated Expiration
2045-05-20

AI Technical Summary

Technical Problem

In existing protein enzymatic hydrolysis reaction systems, the problems of fixed stirring range, uneven shear force distribution and energy consumption in ineffective turbulence lead to reduced enzymatic reaction rate and low mass transfer efficiency.

Method used

The machine uses an adjustable homogenizing component, including a rotating main pipe, a swing plate and a material distribution component. The driving component realizes the lifting and rotation of the rotating main pipe. Combined with the negative pressure effect of the piston column, the stirring range and shear force can be dynamically adjusted to improve the uniformity of material mixing.

Benefits of technology

It realizes dynamic adjustment of stirring range and shear force, improves the efficiency and homogenization effect of enzymatic hydrolysis reaction, solves the limitations of fixed agitators, and improves enzymatic hydrolysis rate and mass transfer efficiency.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention discloses a protein enzymolysis reaction system and process, which relate to the technical field of protein enzymolysis, comprising an enzymolysis tank, wherein a homogenizing component is arranged inside the enzymolysis tank, and a driving component is arranged on the top of the enzymolysis tank, wherein the driving component is used to drive the homogenizing component to lift and rotate, and further comprises a piston column and a material distribution component; the top end of the piston column is fixedly connected to a gantry top frame; when the rotating main pipe moves vertically under the action of the driving component, the provided square pipe can use the end of the fixed pull rod as a leverage point to make the square pipe swing along the connecting cylinder, thereby changing the angle of the connecting cylinder and making the material suction range wider; when the square pipe swings along the connecting cylinder, it can synchronously drive the extended side rod to swing, and with the cooperation of the transmission rod and the long circular groove, the elevation angle of the swing plate is synchronously adjusted, and then when the rotating main pipe drives the swing plate to move up and down, the angle of the swing plate can be changed in real time to ensure the stirring effect of the material.
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Description

Technical Field

[0001] The present invention relates to the technical field of protein enzymolysis, in particular to a protein enzymolysis reaction system and process. Background Art

[0002] In protein enzymatic hydrolysis systems, the stirring mechanism is a core component that influences material mixing homogeneity and hydrolysis efficiency. Existing technologies generally employ fixed stirring designs, such as those using a motor to drive rigid blades or paddles for unidirectional rotation (e.g., the rotating rod and blade structure described in Patent Publication No. CN217265779U). While these designs can achieve basic mixing functions, they have significant limitations in practical applications:

[0003] Fixed stirring range: The fixed geometry and installation angle of traditional stirring components limit their action area to a fixed spatial level within the reactor. When processing high-viscosity protein solutions, localized dead zones are easily formed, resulting in uneven contact between substrate and enzyme molecules, leading to a decrease in the enzymatic reaction rate.

[0004] Uneven shear force distribution: The shear force field generated by the fixed blades is distributed in a gradient. Excessive shear force in the area near the stirring shaft may cause denaturation and inactivation of free enzymes, while insufficient shear force in the edge area cannot fully disperse the aggregated substrate, affecting mass transfer efficiency.

[0005] During enzymatic hydrolysis, substrate concentration, viscosity, and reaction progress continuously change, but fixed agitators are unable to adjust their range and intensity in real time. Furthermore, fixed agitation modes result in some energy being consumed in ineffective turbulence rather than efficient mass transfer. Although recent advances in immobilized enzyme reactors (IMERs) have improved enzyme stability through carrier immobilization strategies, their accompanying agitation systems remain limited by their fixed structure, unable to address the diffusion resistance and local concentration gradients found in heterogeneous systems. Therefore, developing novel agitation mechanisms with dynamically adjustable agitation ranges has become a key approach to addressing the current bottleneck in enzymatic hydrolysis efficiency. Summary of the Invention

[0006] The object of the present invention is to provide a protein enzymatic hydrolysis reaction system and process to solve the problems raised in the above background technology.

[0007] The technical solution of the present invention is: a protein enzymatic hydrolysis reaction system, comprising an enzymatic hydrolysis tank, a homogenizing component disposed inside the enzymatic hydrolysis tank, a driving component disposed on the top of the enzymatic hydrolysis tank, the driving component being used to drive the homogenizing component to lift and rotate, and further comprising;

[0008] A piston column, the top of which is fixedly connected to a gantry top frame, and the gantry top frame is fixedly connected to the top of the enzymolysis tank;

[0009] The homogenizing assembly includes a rotating main pipe, which is movably connected to the top of the enzymolysis tank. The piston column is coaxially arranged with the rotating main pipe, and the bottom end of the piston column is fixedly connected to a plug that matches the inner diameter of the rotating main pipe. The plug is movably connected to the inside of the rotating main pipe. A pair of swing plates are rotatably mounted on the outer peripheral wall of the rotating main pipe.

[0010] A material distribution component is arranged on the inner wall of one end of the enzymolysis tank, and a swinging material suction unit is arranged between the material distribution component and the rotating main pipe.

[0011] Preferably, the material distribution assembly includes an inner ring frame fixedly mounted on the inner wall of one end of the enzymatic hydrolysis tank, and a rotating ring plate is rotatably mounted on the inner ring frame, the cross-section of the rotating ring plate is a right-angled trapezoidal structure, and a plurality of arc grooves are provided on the surface of the rotating ring plate, and the plurality of arc grooves are distributed in an equidistant annular array.

[0012] Preferably, the swinging suction unit includes a pair of connecting short tubes fixedly connected to the rotating main tube, and a one-way valve four is fixedly installed on the connecting short tube. The end of the connecting short tube is rotatably connected to a square tube, and the output direction of the one-way valve four is from the square tube to the rotating main tube. A sealing ring gasket is fixedly installed on the top of the rotating main tube.

[0013] Preferably, the end of each square tube is fixedly connected to a connecting tube, and the connecting tube is provided with evenly distributed filter holes. The outer walls on both sides of each square tube are slidably installed with an adapter seat along its axial direction. The bottom outer wall of the rotating ring plate is fixedly installed with two pairs of inclined fixed rods, and the ends of the fixed rods are rotatably connected to the adapter seat.

[0014] Preferably, an extended side rod is fixedly installed on the outer wall of one side of the two square tubes, and a transmission rod is fixedly installed on the end of the extended side rod. An elongated circular groove is provided on the outer wall of one side of the two swing plates, and the transmission rod is movably fitted with the elongated circular groove. Each of the swing plates is provided with evenly distributed turbulence grooves.

[0015] Preferably, a pair of side pipes are fixedly connected to the rotating main pipe, and a one-way valve three is fixedly installed on one end of the side pipe near the bottom, and the output direction of the one-way valve three is from the bottom end of the side pipe to the rotating main pipe. A stiffening plate is fixedly installed on the outer wall of the two side pipes and the rotating main pipe. A pair of branch pipes are fixedly connected to one end of the rotating main pipe near the top, and a one-way valve two is fixedly installed on each branch pipe, and the output direction of the one-way valve two is toward the rotating main pipe.

[0016] Preferably, the bottom end of the rotating main pipe is fixedly connected to a fan-shaped plate, and a tree-shaped diversion groove connected to the rotating main pipe is opened in the fan-shaped plate. A one-way valve is fixedly installed at one end of the rotating main pipe close to the fan-shaped plate, and the output direction of the one-way valve is from the rotating main pipe to the tree-shaped diversion groove.

[0017] Preferably, the drive assembly includes a pair of electric push rods fixedly mounted on the top of the enzymolysis tank, and the ends of the extension rods of the two electric push rods are jointly fixedly mounted with a mounting plate, and the mounting plate is rotatably connected to the rotating main pipe through a bearing, a supporting outer frame is fixedly mounted on the outer peripheral wall of the enzymolysis tank, and one end of the outer peripheral wall of the enzymolysis tank close to the top is fixedly connected to a feed pipe, and the bottom end of the enzymolysis tank is fixedly connected to a discharge pipe with a valve.

[0018] Preferably, driven conical teeth are fixedly mounted on the outer peripheral wall of the rotating main pipe, a driving motor is fixedly mounted on the mounting plate, and driving conical teeth are fixedly mounted on the output shaft of the driving motor, and the driving conical teeth are meshed with the driven conical teeth.

[0019] The present invention also discloses a protein enzymatic hydrolysis reaction process, which is applied to the protein enzymatic hydrolysis reaction system and comprises the following steps:

[0020] S1. Prepare the meat. Choose beef tenderloin or beef leg. Remove the fascia completely and cut the meat into 3cm pieces. 3 -5cm 3 small pieces, then mince them into a paste and mix them into a slurry;

[0021] S2. Add the material prepared in S1 to the enzymatic hydrolysis tank, and select protease, add the protease to warm water to dissolve it, and then add it to the enzymatic hydrolysis tank;

[0022] S3. Using a homogenizing component, fully mix the dissolved protease with the material;

[0023] S4. Use an enzymatic hydrolysis tank to perform enzymatic hydrolysis of the protein, with the enzymatic hydrolysis temperature set to 40°C-60°C, the substrate solid-liquid ratio to 1:5 to 1:10, the amount of protease added to 2%-5%, and the enzymatic hydrolysis time to 2h-4h.

[0024] The present invention provides a protein enzymatic hydrolysis reaction system and process through improvements, which have the following improvements and advantages compared with the prior art:

[0025] First, when the rotating main pipe of the present invention moves vertically under the action of the driving assembly, the provided square pipe can use the end of the fixed pull rod as a lever point to make the square pipe swing along the connecting pipe, thereby changing the angle of the connecting pipe and making the material suction range wider;

[0026] Secondly, when the square tube of the present invention swings along the connecting tube, it can synchronously drive the extended side rod to swing, and the transmission rod and the long circular groove cooperate to synchronously adjust the elevation angle of the swing plate. Then, when the rotating main tube drives the swing plate to move up and down, the angle of the swing plate can change in real time, thereby ensuring the stirring effect of the material;

[0027] Thirdly, when the rotating main pipe moves downward, the piston column and the plug are fixedly arranged, so the space at the bottom of the piston column increases, thereby utilizing the negative pressure effect to extract the liquid in the enzymatic hydrolysis tank through the filter through-hole on the connecting tube. At the same time, the space at the top of the piston column decreases, so that the air above the piston column is pressed into the lower end of the rotating main pipe through the side pipe and the one-way valve, further improving the cavitation effect on the material. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the specific embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0029] Figure 1 It is a schematic diagram of the overall three-dimensional structure of the present invention;

[0030] Figure 2 For the present invention Figure 1 A in the middle is an enlarged structural diagram;

[0031] Figure 3 It is a schematic diagram of the partial cross-section structure of the enzymolysis tank of the present invention;

[0032] Figure 4 This is a schematic diagram of the half-section structure of the enzymatic hydrolysis tank of the present invention;

[0033] Figure 5 This is a schematic diagram of the three-dimensional structure of the stirring assembly of the present invention;

[0034] Figure 6 For the present invention Figure 5 The enlarged structural diagram at B in the middle;

[0035] Figure 7 For the present invention Figure 5 The enlarged structural diagram at C in the middle;

[0036] Figure 8 Schematic diagram of the three-dimensional structure of the swing plate of the present invention;

[0037] Figure 9 It is a schematic diagram of the three-dimensional structure of the rotating ring plate of the present invention.

[0038] Reference numerals:

[0039] 1. Enzyme hydrolysis tank; 101. Support frame; 102. Discharge pipe with valve; 103. Feed pipe; 2. Electric push rod; 201. Mounting plate; 3. Gantry top frame; 301. Piston column; 302. Sealing ring gasket; 303. Plug; 4. Rotating main pipe; 401. Driven tapered gear; 402. One-way valve 1; 5. Drive motor; 501. Drive tapered gear; 6. Inner ring frame; 601. Rotating ring plate; 602. Arc groove ;603, fixed pull rod; 7, side pipe; 701, one-way valve three; 8, branch pipe; 801, one-way valve two; 9, swing plate; 901, oblong groove; 902, turbulence groove; 10, square tube; 11, connecting cylinder; 111, filter through hole; 12, fan-shaped plate; 121, tree-shaped diversion groove; 13, connecting short pipe; 131, one-way valve four; 14, extended side rod; 141, transmission rod; 15, stiffening plate; 16, adapter seat. DETAILED DESCRIPTION

[0040] The present invention is described in detail below, clearly and completely describing the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0041] The present invention provides a protein enzymatic hydrolysis reaction system and process through improvement. The technical solution of the present invention is:

[0042] like Figures 1 to 9 As shown, an embodiment of the present invention provides a protein enzymatic hydrolysis reaction system, including an enzymatic hydrolysis tank 1, wherein a homogenizing component is provided inside the enzymatic hydrolysis tank 1, and a driving component is provided on the top of the enzymatic hydrolysis tank 1, wherein the driving component is used to drive the homogenizing component to move up and down and rotate. When the homogenizing component moves vertically, the operating range can be increased, thereby achieving the purpose of improving the stirring effect, and further comprising;

[0043] The piston column 301, the top of the piston column 301 is fixedly connected to the gantry top frame 3, and the gantry top frame 3 is fixedly connected to the top of the enzymolysis tank 1;

[0044] The homogenizing assembly includes a rotating main pipe 4, which is movably connected to the top of the enzymolysis tank 1. A piston column 301 is coaxially arranged with the rotating main pipe 4, and a plug column 303 adapted to the inner diameter of the rotating main pipe 4 is fixedly connected to the bottom end of the piston column 301. The plug column 303 is movably connected to the inside of the rotating main pipe 4. A pair of swing plates 9 are rotatably mounted on the outer peripheral wall of the rotating main pipe 4.

[0045] The material distribution component is arranged on the inner wall of one end of the enzymatic hydrolysis tank 1, and a swinging material suction unit is arranged between the material distribution component and the rotating main pipe 4; when the driving component controls the rotating main pipe 4 to move in the vertical direction, the swinging material suction unit can suck and discharge the material to promote the full mixing of the protease and the material.

[0046] Further, such as Figure 2-Figure 5 As shown, the material distribution component includes an inner ring frame 6 fixedly mounted on the inner wall of one end of the enzymolysis tank 1, and a rotating plate 601 is rotatably mounted on the inner ring frame 6, and the cross-section of the rotating plate 601 is a right-angled trapezoidal structure, and a plurality of arc grooves 602 are provided on the surface of the rotating plate 601, and the plurality of arc grooves 602 are distributed in an equidistant annular array; through the above structure, the material added to the enzymolysis tank 1 will first fall on the rotating plate 601, and then be diverted through the plurality of arc grooves 602, and finally, with the rotation of the rotating plate 601, the purpose of uniform feeding can be achieved.

[0047] As a further solution of the present invention, the swinging suction unit includes a pair of connecting short tubes 13 fixedly connected to the rotating main tube 4, and a one-way valve four 131 is fixedly installed on the connecting short tube 13. The end of the connecting short tube 13 is rotatably connected to the square tube 10. The output direction of the one-way valve four 131 is transmitted from the square tube 10 to the rotating main tube 4. A sealing ring gasket 302 is fixedly installed on the top of the rotating main tube 4.

[0048] Furthermore, the end of each square tube 10 is fixedly connected to a connecting tube 11, and the connecting tube 11 is provided with evenly distributed filtering holes 111. The outer walls on both sides of each square tube 10 are slidably installed with an adapter seat 16 along its axial direction, and the bottom outer wall of the rotating ring plate 601 is fixedly installed with two pairs of inclined fixed rods 603, and the ends of the fixed rods 603 are rotatably connected to the adapter seat 16.

[0049] Through the above structure, when the rotating main pipe 4 rotates, it can drive the square pipe 10 to rotate, and at the same time, the adapter seat 16 and the fixed pull rod 603 provided can drive the rotating ring plate 601 to rotate, thereby realizing the mixing operation and the material separation process during the feeding process;

[0050] At the same time, when the rotating main pipe 4 moves vertically under the action of the driving assembly, the set square pipe 10 can use the end of the fixed pull rod 603 as a leverage point, so that the square pipe 10 swings along the connecting pipe 11, thereby changing the angle of the connecting pipe 11 and making the material suction range wider.

[0051] As a further solution of the present invention, an extended side rod 14 is fixedly installed on the outer wall of one side of the two square tubes 10, and a transmission rod 141 is fixedly installed on the end of the extended side rod 14. An elongated circular groove 901 is provided on the outer wall of one side of the two swing plates 9, and the transmission rod 141 is movably matched with the elongated circular groove 901. Each swing plate 9 is provided with evenly distributed turbulence grooves 902.

[0052] Through the above structure, such as Figure 5-Figure 8 As shown, when the square tube 10 swings along the connecting tube 11, it can synchronously drive the extended side rod 14 to swing, and with the cooperation of the transmission rod 141 and the oblong groove 901, the elevation angle of the swing plate 9 is synchronously adjusted. Then, when the rotating main pipe 4 drives the swing plate 9 to move up and down, the angle of the swing plate 9 can change in real time, thereby ensuring the stirring effect of the material.

[0053] Furthermore, a pair of side tubes 7 are fixedly connected to the rotating main pipe 4, and a one-way valve 3 701 is fixedly installed at one end of the side tube 7 near the bottom. The output direction of the one-way valve 3 701 is output from the bottom end of the side tube 7 to the rotating main pipe 4. A stiffening plate 15 is fixedly installed on the outer wall of the two side tubes 7 and the rotating main pipe 4. A pair of branch tubes 8 are fixedly connected to one end of the rotating main pipe 4 near the top, and a one-way valve 2 801 is fixedly installed on each branch tube 8, and the output direction of the one-way valve 2 801 is toward the rotating main pipe 4. Through the above structure, when the rotating main pipe 4 moves downward, since the piston column 301 and the plug column 303 are fixedly arranged, the space at the bottom of the piston column 301 increases, thereby utilizing the negative pressure effect to extract the liquid in the enzymatic hydrolysis tank 1 through the filter through hole 111 on the connecting tube 11. At the same time, the space at the top of the piston column 301 decreases, so that the air above the piston column 301 is pressed into the lower end of the rotating main pipe 4 through the side tube 7 and the one-way valve 3 701.

[0054] When the rotating main pipe 4 moves upward, the space at the bottom of the piston column 301 decreases, thereby transferring the sucked liquid and air through the one-way valve 1 402 to the subsequent sector plate 12 and bulging out through the tree-like diversion grooves 121, thereby utilizing cavitation to achieve the purpose of improving the homogenization effect. In addition, the space at the top of the piston column 301 increases, and the negative pressure effect is used to re-extract air into the rotating main pipe 4 through the branch pipe 8 and the one-way valve 2 801.

[0055] As a further embodiment of the present invention, Figure 3-Figure 5As shown, the bottom end of the rotating main pipe 4 is fixedly connected to the fan-shaped plate 12, and the fan-shaped plate 12 is provided with a tree-shaped diversion groove 121 connected to the rotating main pipe 4. A one-way valve 402 is fixedly installed at one end of the rotating main pipe 4 close to the fan-shaped plate 12, and the output direction of the one-way valve 402 is output from the rotating main pipe 4 to the tree-shaped diversion groove 121; through the above structure, when the rotating main pipe 4 rotates, it can drive the fan-shaped plate 12 to rotate, playing a role in auxiliary stirring; and the provided tree-shaped diversion groove 121 can re-divert air and material, further improving the cavitation effect on the material.

[0056] As a further embodiment of the present invention, Figure 1 、 Figure 2 as well as Figure 6 As shown, the driving assembly includes a pair of electric push rods 2 fixedly mounted on the top of the enzymolysis tank 1, and the ends of the extension rods of the two electric push rods 2 are jointly fixedly mounted with a mounting plate 201, and the mounting plate 201 is rotatably connected to the rotating main pipe 4 through a bearing, a supporting outer frame 101 is fixedly mounted on the outer peripheral wall of the enzymolysis tank 1, and one end of the outer peripheral wall of the enzymolysis tank 1 near the top is fixedly connected to a feed pipe 103, and the bottom end of the enzymolysis tank 1 is fixedly connected to a valved discharge pipe 102; after the protein enzymolysis is completed, the valve on the valved discharge pipe 102 can be opened to perform unloading operations.

[0057] Furthermore, a driven conical tooth 401 is fixedly mounted on the outer peripheral wall of the rotating main tube 4 , a driving motor 5 is fixedly mounted on the mounting plate 201 , and a driving conical tooth 501 is fixedly mounted on the output shaft of the driving motor 5 , and the driving conical tooth 501 is meshed with the driven conical tooth 401 .

[0058] Through the above structure, the electric push rod 2 is provided, which can cooperate with the mounting plate 201 to drive the rotating main pipe 4 to move vertically. At the same time, when the driving motor 5 is controlled to start, the driving conical teeth 501 and the driven conical teeth 401 can drive the rotating main pipe 4 to rotate to perform the stirring operation.

[0059] The specific working method is as follows: when in use, the driving motor 5 is controlled to start, and the driving conical gear 501 and the driven conical gear 401 are driven to rotate, and when the rotating main pipe 4 rotates, the square tube 10 can be driven to rotate, and at the same time, the adapter 16 and the fixed pull rod 603 are coordinated to drive the rotating plate 601 to rotate. The material added to the enzymatic hydrolysis tank 1 will first fall on the rotating plate 601, and then be diverted through the multiple arc grooves 602 provided. Finally, the rotation of the rotating plate 601 is coordinated to achieve the purpose of uniform feeding.

[0060] When the rotating main pipe 4 moves vertically under the action of the driving assembly, the provided square pipe 10 can use the end of the fixed pull rod 603 as a lever point to make the square pipe 10 swing along the connecting pipe 11, thereby changing the angle of the connecting pipe 11, making the material suction range wider; when the square pipe 10 swings along the connecting pipe 11, it can synchronously drive the extended side rod 14 to swing, and with the cooperation of the transmission rod 141 and the oblong groove 901, the elevation angle of the swing plate 9 is synchronously adjusted, and then when the rotating main pipe 4 drives the swing plate 9 to move up and down, the angle of the swing plate 9 can change in real time, thereby ensuring the stirring effect of the material;

[0061] When the rotating main pipe 4 moves downward, since the piston column 301 and the plug column 303 are fixed, the space at the bottom of the piston column 301 increases, thereby utilizing the negative pressure effect to extract the liquid in the enzymolysis tank 1 through the filter through hole 111 on the connecting tube 11. At the same time, the space at the top of the piston column 301 decreases, thereby pressing the air above the piston column 301 into the lower end of the rotating main pipe 4 through the side pipe 7 and the one-way valve 3 701; when the rotating main pipe 4 moves upward, the space at the bottom of the piston column 301 decreases. , thereby transmitting the sucked liquid and air to the subsequent fan-shaped plate 12 through the one-way valve 402, and bulging out through the tree-like diversion groove 121, thereby utilizing the cavitation effect to achieve the purpose of improving the homogenization effect; in addition, the space on the top of the piston column 301 is increased, and the negative pressure effect is utilized to re-extract air into the rotating main pipe 4 through the branch pipe 8 and the one-way valve 801. The above operation can achieve effective homogenization of the material; after the protein enzymatic hydrolysis is completed, the valve on the valved discharge pipe 102 can be opened to carry out the unloading operation.

[0062] Example 2

[0063] This embodiment discloses a protein enzymatic hydrolysis reaction process, comprising the following steps:

[0064] S1. Prepare the meat. Choose beef tenderloin or beef leg. Remove the fascia completely and cut the meat into 3cm pieces. 3 -5cm 3 small pieces, then mince them into a paste and mix them into a slurry;

[0065] S2, adding the material prepared in S1 to the enzymolysis tank 1, and selecting protease, adding the protease to warm water for dissolution, and then adding it to the enzymolysis tank 1;

[0066] S3. Using a homogenizing component, fully mix the dissolved protease with the material;

[0067] S4. Use enzymolysis tank 1 to perform enzymatic hydrolysis of protein, set the enzymatic hydrolysis temperature to 40°C-60°C, the substrate solid-liquid ratio to 1:5 to 1:10, the amount of protease added to 2%-5%, and the enzymatic hydrolysis time to 2h-4h.

[0068] The above description is intended to enable one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not limited to the embodiments shown herein but is intended to conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A protein enzymatic hydrolysis reaction system, comprising an enzymatic hydrolysis tank (1), characterized in that: The enzymolysis tank (1) is provided with a homogenizing component inside, and a driving component is provided on the top of the enzymolysis tank (1), and the driving component is used to drive the homogenizing component to lift and rotate, and further includes; A piston column (301), wherein the top of the piston column (301) is fixedly connected to a gantry top frame (3), and the gantry top frame (3) is fixedly connected to the top of the enzymolysis tank (1); The homogenizing assembly comprises a rotating main pipe (4), the rotating main pipe (4) is movably connected to the top of the enzymolysis tank (1), the piston column (301) is coaxially arranged with the rotating main pipe (4), and the bottom end of the piston column (301) is fixedly connected to a plug column (303) adapted to the inner diameter of the rotating main pipe (4), and the plug column (303) is movably connected to the inside of the rotating main pipe (4), and a pair of swing plates (9) are rotatably mounted on the outer peripheral wall of the rotating main pipe (4); A material distribution component is provided on the inner wall of one end of the enzymolysis tank (1); a swinging material suction unit is provided between the material distribution component and the rotating main pipe (4); the swinging material suction unit comprises a pair of connecting short pipes (13) fixedly connected to the rotating main pipe (4); and a one-way valve (131) is fixedly installed on the connecting short pipe (13); the end of the connecting short pipe (13) is connected to the square pipe (10) through rotation; the output direction of the one-way valve (131) is transmitted from the square pipe (10) to the rotating main pipe (4); and a sealing ring gasket (302) is fixedly installed on the top of the rotating main pipe (4); The rotating main pipe (4) is fixedly connected to a pair of side pipes (7), and a one-way valve (701) is fixedly installed on one end of the side pipe (7) near the bottom, and the output direction of the one-way valve (701) is output from the bottom end of the side pipe (7) to the rotating main pipe (4), and a stiffening plate (15) is fixedly installed on the outer wall of the two side pipes (7) and the rotating main pipe (4). The rotating main pipe (4) is fixedly connected to one end of the side pipe (7) near the top, and a one-way valve (8) is fixedly installed on each branch pipe (8), and the output direction of the one-way valve (801) is toward the rotating main pipe (4); The bottom end of the rotating main pipe (4) is fixedly connected to a sector plate (12), and a tree-shaped diversion groove (121) is provided in the sector plate (12) and is in communication with the rotating main pipe (4). A one-way valve (402) is fixedly installed at one end of the rotating main pipe (4) close to the sector plate (12), and the output direction of the one-way valve (402) is transmitted from the rotating main pipe (4) to the tree-shaped diversion groove (121).

2. A protein enzymatic hydrolysis reaction system according to claim 1, characterized in that: The material distribution assembly comprises an inner ring frame (6) fixedly mounted on the inner wall of one end of the enzymolysis tank (1), and a rotating plate (601) is rotatably mounted on the inner ring frame (6), the cross section of the rotating plate (601) being a right-angled trapezoidal structure, and a plurality of arc-shaped grooves (602) are formed on the surface of the rotating plate (601), and the plurality of arc-shaped grooves (602) are distributed in an equidistant annular array.

3. A protein enzymatic hydrolysis reaction system according to claim 2, characterized in that: The end of each square tube (10) is fixedly connected to a connecting tube (11), and the connecting tube (11) is provided with evenly distributed filtering holes (111). The outer walls on both sides of each square tube (10) are slidably mounted with an adapter seat (16) along the axial direction thereof. The bottom outer wall of the rotating ring plate (601) is fixedly mounted with two pairs of inclined fixed rods (603), and the ends of the fixed rods (603) are rotatably connected to the adapter seat (16).

4. A protein enzymatic hydrolysis reaction system according to claim 3, characterized in that: An extension side rod (14) is fixedly mounted on one side outer wall of each of the two square tubes (10), and a transmission rod (141) is fixedly mounted on the end of each of the extension side rods (14). An elongated circular groove (901) is provided on one side outer wall of each of the two swing plates (9), and the transmission rod (141) is movably engaged with the elongated circular groove (901). Each of the swing plates (9) is provided with evenly distributed turbulence grooves (902).

5. A protein enzymatic hydrolysis reaction system according to claim 1, characterized in that: The driving assembly comprises a pair of electric push rods (2) fixedly mounted on the top of the enzymolysis tank (1), and the ends of the extension rods of the two electric push rods (2) are fixedly mounted with a mounting plate (201), and the mounting plate (201) is rotatably connected to the rotating main pipe (4) through a bearing, a supporting outer frame (101) is fixedly mounted on the outer peripheral wall of the enzymolysis tank (1), and an end of the outer peripheral wall of the enzymolysis tank (1) close to the top is fixedly connected to a feed pipe (103), and a bottom end of the enzymolysis tank (1) is fixedly connected to a discharge pipe (102) with a valve.

6. A protein enzymatic hydrolysis reaction system according to claim 5, characterized in that: A driven conical tooth (401) is fixedly mounted on the outer peripheral wall of the rotating main pipe (4), a driving motor (5) is fixedly mounted on the mounting plate (201), and a driving conical tooth (501) is fixedly mounted on the output shaft of the driving motor (5), and the driving conical tooth (501) and the driven conical tooth (401) are meshed.

7. A protein enzymatic hydrolysis reaction process, applied to the protein enzymatic hydrolysis reaction system according to any one of claims 1 to 6, characterized in that: The following steps are involved: S1. Prepare the meat. Choose beef tenderloin or beef leg. Remove the fascia completely and cut the meat into 3cm pieces. 3 -5cm 3 small pieces, and then mince them into a paste and mix them into a homogenous slurry; S2, add the material prepared in S1 to the enzymatic hydrolysis tank (1), and select protease, add the protease to warm water to dissolve it, and then add it to the enzymatic hydrolysis tank (1); S3, use a homogenizing component to fully mix the dissolved protease with the material; S4, use the enzymatic hydrolysis tank (1) to perform an enzymatic hydrolysis reaction of the protein, and set the enzymatic hydrolysis temperature to 40℃-60℃, the substrate solid-liquid ratio to 1:5 to 1:10, the protease addition amount to 2%-5%, and the enzymatic hydrolysis time to 2h-4h.

Citation Information

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