Full-automatic shutdown cleaning method of flow cytometer and automatic connector switching device

Abstract

The invention provides a full-automatic shutdown cleaning method of a flow cytometer and an automatic connector switching device, the full-automatic shutdown cleaning method of the flow cytometer automatically carries out a shutdown cleaning process in the whole process without personnel on duty and operation, and the automatic connector switching device is utilized in the shutdown cleaning process and comprises a bottom plate, an automatic connector switching device and a controller, the sheath liquid outlet female plug and the shutdown liquid outlet female plug are mounted on the shell; the clamping jaw assembly is used for clamping the sheath fluid pipe male connector; the rotating assembly is used for driving the clamping jaw assembly to rotate between the sheath fluid outlet female plug and the shutdown fluid outlet female plug; and the vertical movement assembly is used for driving the clamping jaw assembly to move up and down, the automatic connector switching device automatically switches the sheath fluid pipe male connector between the sheath fluid outlet female plug and the shutdown fluid outlet female plug, and the full-automatic unattended shutdown process is achieved.

Description

Technical Field

[0001] The present application relates to the technical field of life science detection equipment, and in particular to a fully automatic shutdown and cleaning method for a flow cytometer and an automatic switching connector device. Background Art

[0002] Flow cytometers are widely used in toxicology, cell biology, immunology, microbiology and other related fields, and have become indispensable instruments and equipment for many scientific research units. Analytical flow cytometers are mainly composed of four parts: flow chamber and liquid flow system; laser source and optical system; photoelectric tube and detection system; computer and analysis system. The flow chamber and liquid flow system are mainly responsible for confining the cell or particle suspension sample through the sheath fluid, so that the cells or particles flow through the laser focused detection point one by one, generating multiple different parameter signals, which are collected by the detection system for subsequent analysis.

[0003] Cleanliness is very important for fluidic components including the flow chamber. Many flow cytometer manufacturers have established their own shutdown cleaning procedures, such as Agile ent Novocyte, BD FACSVia, Accur i C6 Plus, ThermoFisher Attune NxT, Amnis FlowSign, SinoCyte, FlowCyte, etc.

[0004] In the normal shutdown process of the current instrument, the user needs to first enter the equipment maintenance interface and then click to turn off the liquid flow. After that, the user needs to follow the prompts of the software to perform the shutdown operation step by step. There is a possibility of errors during operation. The user needs to be on duty throughout the shutdown process and can only leave after the shutdown process is completed. Summary of the invention

[0005] The present application proposes a fully automatic shutdown and cleaning method for a flow cytometer and an automatic switching connector device, which realizes a fully automatic unattended shutdown process, reduces the possibility of human error and reduces the manual maintenance work of the instrument.

[0006] The present application provides an automatic switching connector device, which is used to automatically switch the male connector of a sheath fluid tube between a sheath fluid outlet female plug and a shutdown fluid outlet female plug, and includes: a base plate, on which the sheath fluid outlet female plug and the shutdown fluid outlet female plug are installed; a clamping jaw assembly, which is used to clamp the male connector of the sheath fluid tube; a rotating assembly, which is used to drive the clamping jaw assembly to rotate between the sheath fluid outlet female plug and the shutdown fluid outlet female plug; and a vertical motion assembly, which is used to drive the clamping jaw assembly to move up and down.

[0007] Specifically, the rotating assembly includes: a rotating arm, a rotating cylinder, and a rotating cylinder fixing plate. The rotating cylinder fixing plate is used to mount the rotating cylinder on the vertical movement assembly; the rotating cylinder is used to drive the rotating arm to rotate around the rotating cylinder as the center; the rotating arm is fixed to the jaw assembly, driving the jaw assembly to rotate in a circle around the rotating cylinder as the center with the rotating arm as the axis.

[0008] Specifically, the vertical movement assembly includes: an up-and-down movement cylinder and an up-and-down cylinder fixing plate. The up-and-down cylinder fixing plate is used to mount the up-and-down movement cylinder on the bottom plate; the up-and-down movement cylinder is used to drive the rotating assembly and the jaw assembly to move up and down.

[0009] Specifically, the jaw assembly includes two side jaws and a jaw cylinder. The jaws are used to grip or release the sheath fluid tube male connector; the jaw cylinder is used to drive the opening and closing of the two side jaws.

[0010] Specifically, a positioning boss is provided on the bottom plate. The positioning boss is used to limit the orientation of the connector insertion / removal buttons of the sheath fluid outlet female plug and the shutdown fluid outlet female plug.

[0011] Specifically, a downward pressure avoidance angle is provided on the inner side of the bottom of the jaw on the same side as the connector insertion / removal button. The downward pressure avoidance angle is used to prevent the jaw assembly from touching the connector insertion / removal buttons of the sheath fluid outlet female plug and the shutdown fluid outlet female plug when pressing down.

[0012] This application also provides a fully automatic shutdown and cleaning method for a flow cytometer, including: S1, the flow cytometer receives a shutdown command; S2, the residual sample in the sample chamber is pumped back into the waste liquid bucket, and the cleaning liquid is automatically loaded into the sample chamber. The cleaning liquid flows from the sample chamber through the sample pipeline to the flow chamber; S3, the sheath fluid in the flow chamber flows through the sample pipeline to the sample chamber; S4, it is judged whether the liquid volume in the shutdown liquid bucket reaches a preset value; S5, when it is determined that the liquid volume does not reach the preset value, the valve is opened to supplement the shutdown liquid until it reaches the preset value; S6, when it is determined that the liquid volume reaches the preset value, the automatic switching joint device is started to work, and the sheath fluid tube male connector is transferred from the sheath fluid outlet female plug to the shutdown fluid outlet female plug; S7, the shutdown liquid flows from the shutdown liquid bucket through the sheath fluid pipeline to the flow chamber, and the shutdown liquid in the flow chamber is backflushed and rinsed into the sample pipeline; S8, it is judged the cleaning effect in the sheath fluid pipeline and the sample pipeline; S9, when it is determined that the cleaning effect does not meet the standard, steps S2 to S7 are repeated until the cleaning effect meets the standard; S10, when it is determined that the cleaning effect meets the standard, the liquid flow is closed and the flow cytometer is automatically shut down; wherein, the automatic switching joint device is the automatic switching joint device in any one of the above.

[0013] Specifically, the cleaning effects in the sheath fluid pipeline and the sample pipeline are verified through cross - contamination rate tests or aseptic culture tests.

[0014] Specifically, the method determines whether the liquid volume in the shutdown liquid barrel reaches a preset value by judging whether the percentage of the liquid volume in the shutdown liquid barrel in the volume of the shutdown liquid barrel reaches a preset value through a weighing sensor.

[0015] Compared with the prior art, the present application proposes an automatic switching joint device and a fully - automatic shutdown cleaning method for a flow cytometer. After a shutdown instruction is issued, the flow cytometer starts to perform liquid replacement, rinsing, and automatically switches joints through the automatic switching joint device in sequence in a fully - automatic manner. The automatic switching joint device clamps the male joint of the sheath fluid pipe through a jaw assembly, rotates the jaw assembly between the female plug of the sheath fluid outlet and the female plug of the shutdown liquid outlet through a rotating assembly, and uses a vertical movement assembly to drive the jaw assembly to move up and down. Thus, the automatic switching joint device realizes the automatic switching of the male joint of the sheath fluid pipe between the female plug of the sheath fluid outlet and the female plug of the shutdown liquid outlet. It realizes a fully - automatic unattended shutdown process, reduces the possibility of human misoperation, eliminates the need for equipment maintenance personnel, and reduces the maintenance work of the instrument. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0017] Figure 1 is a schematic flow chart of the shutdown cleaning of a prior - art flow cytometer;

[0018] Figure 2 is a schematic flow chart of the fully - automatic shutdown cleaning method of the flow cytometer in the embodiment of the present application;

[0019] Figure 3 is a schematic diagram of the automatic switching joint device in the embodiment of the present application;

[0020] Figure 4 is a schematic diagram of the positioning boss in the embodiment of the present application;

[0021] Figure 5 is a schematic diagram of the jaw in the embodiment of the present application. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0022] The embodiments of the present application will be described in detail below with reference to the drawings.

[0023] The following describes the implementation manners of the present application through specific specific examples. Those skilled in the art can easily understand other advantages and effects of the present application from the content disclosed in this specification. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. The present application can also be implemented or applied through other different specific implementation manners. Various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present application. It should be noted that, without conflict, the following embodiments and the features in the embodiments can be combined with each other. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope protected by the present application.

[0024] It should be noted that the following describes various aspects of the embodiments within the scope of the appended claims. It should be obvious that the aspects described herein can be embodied in a wide variety of forms, and any specific structure and / or function described herein is illustrative only. Based on the present application, those skilled in the art should understand that one aspect described herein can be implemented independently of any other aspect, and two or more of these aspects can be combined in various ways. For example, any number and aspects described herein can be used to implement the device and / or practice the method. Additionally, this device and / or method can be implemented using other structures and / or functions in addition to one or more of the aspects described herein.

[0025] It should also be noted that the diagrams provided in the following embodiments only illustrate the basic concept of the present application schematically. The diagrams only show the components related to the present application, rather than being drawn according to the number, shape, and size of the components in actual implementation. The type, quantity, and proportion of each component in its actual implementation can be arbitrarily changed, and the component layout type may also be more complex.

[0026] In addition, in the following description, specific details are provided for the purpose of facilitating a thorough understanding of the examples. However, those skilled in the art will understand that the examples can be practiced without these specific details.

[0027] Currently, in the normal shutdown process of a flow cytometer, the user needs to first enter the device maintenance interface, then click to close the liquid flow. After that, the user needs to follow the prompts of the software and perform each step in the shutdown operation in sequence, such as Figure 1 As shown, the process is as follows:

[0028] S11, the user clicks the shutdown button, and the flow cytometer receives the shutdown command.

[0029] S12, the user empties the remaining sample in the sample chamber according to the software prompt and adds 4 ml of cleaning liquid to the sample chamber.

[0030]

[0030] After the user finishes adding the cleaning solution and clicks the load confirmation button, the cleaning solution flows from the sample chamber through the sample pipeline to the flow chamber.

[0031] After the user waits for the cleaning solution to finish flowing and clicks the cleaning completion button, the sheath fluid in the flow chamber flows through the sample pipeline to the sample chamber.

[0032]

[0031] The user checks the shutdown liquid barrel according to the software prompt to ensure that the liquid in the shutdown liquid barrel is more than half.

[0033] After the user ensures that the liquid in the shutdown liquid barrel is more than half, the sheath fluid tube is pulled out and connected to the shutdown liquid barrel.

[0034]

[0032] After the user waits for the cleaning of the sheath fluid pipeline and the sample pipeline to be completed, clicks the shutdown button on the software interface to complete the entire shutdown process.

[0035] In the above shutdown process, the user needs to be on duty throughout the shutdown process. The user needs to perform corresponding operations step by step according to the software interface prompts throughout the process. And during the shutdown process, the user needs to manually switch the cleaning barrel and its pipeline joints in the corresponding steps. Only after waiting for the shutdown process to end can the user leave.

[0036]

[0033] ]In summary, the existing shutdown process of the flow cytometer requires full-time manual attendance and operation, and there is a possibility of human error during the attendance operation.

[0037] For this reason, the embodiment of the present application provides a fully automatic shutdown and cleaning method for a flow cytometer. The fully automatic shutdown and cleaning method of the flow cytometer can realize a fully automatic unattended shutdown process, reduce the possibility of human error, and reduce the maintenance work of the instrument. The user can only be equipped with operators and does not need to configure equipment maintenance personnel. This increases the competitiveness of the product.

[0038] As Figure 2

[0034] shown, the fully automatic shutdown and cleaning method of the flow cytometer of the present application includes:

[0039] S1. The flow cytometer receives a shutdown command.

[0040]

[0035] S2. The residual sample in the sample chamber is pumped back into the waste liquid barrel, the cleaning solution is automatically loaded into the sample chamber, and the cleaning solution flows from the sample chamber through the sample pipeline to the flow chamber.

[0041] S3. The sheath fluid in the flow chamber flows through the sample pipeline to the sample chamber.

[0042]

[0036] S4. Determine whether the liquid volume in the shutdown liquid barrel reaches a preset value.

[0043] S5. When it is determined that the liquid volume has not reached the preset value, open the valve to supplement the shutdown liquid until the preset value is reached.

[0044] S6. When it is determined that the liquid volume has reached the preset value, automatically switch the joint device to start working, and transfer the male joint of the sheath fluid tube from the female plug of the sheath fluid outlet to the female plug of the shutdown liquid outlet.

[0045] S7. The shutdown liquid flows from the shutdown liquid barrel through the sheath fluid pipeline to the flow chamber, and the shutdown liquid in the flow chamber backflushes and rinses into the sample pipeline.

[0046] S8. Judge the cleaning effect in the sheath fluid pipeline and the sample pipeline.

[0047] S9. When it is determined that the cleaning effect does not meet the standard, repeat steps S2 - S7 until the cleaning effect meets the standard.

[0048] S10. When it is determined that the cleaning effect meets the standard, turn off the liquid flow and automatically shut down.

[0049] In step S6, the embodiment of the present application provides an automatic switching joint device. This automatic switching joint device can replace workers and realize the automatic switching of the male joint of the sheath fluid tube between the female plug of the sheath fluid outlet and the female plug of the shutdown liquid outlet in the full - automatic shutdown cleaning method of the flow cytometer.

[0050] As Figure 3 shown, the automatic switching joint device 10 includes a bottom plate 11, a jaw assembly 12, a rotating assembly 13, and a vertical movement assembly 14.

[0051] The bottom plate 11 is installed with a female plug 111 of the sheath fluid outlet and a female plug 112 of the shutdown liquid outlet. A sheath fluid barrel is connected below the female plug 111 of the sheath fluid outlet, and the pipeline can be connected to the inside of the sheath fluid barrel through the connection with the female plug 111 of the sheath fluid outlet. A shutdown liquid barrel is connected below the female plug 112 of the shutdown liquid outlet, and the pipeline can be connected to the inside of the shutdown liquid barrel through the connection with the female plug 112 of the shutdown liquid outlet.

[0052] The jaw assembly 12 is used to clamp the male joint 121 of the sheath fluid tube, and the male joint 121 of the sheath fluid tube is matched and connected with the female plug 111 of the sheath fluid outlet or the female plug 112 of the shutdown liquid outlet.

[0053] The jaw assembly 12 clamps the male joint 121 of the sheath fluid tube connected to the female plug 111 of the sheath fluid outlet. After the jaw assembly 12 connects the clamped male joint 121 of the sheath fluid tube with the female plug 112 of the shutdown liquid outlet, it releases the male joint 121 of the sheath fluid tube, completing the transfer of the male joint 121 of the sheath fluid tube from the female plug 111 of the sheath fluid outlet to the female plug 112 of the shutdown liquid outlet.

[0054] Instead, the jaw assembly 12 can grip the sheath liquid tube male connector 121 connected to the shutdown liquid outlet female plug 112. After connecting the gripped sheath liquid tube male connector 121 to the sheath liquid outlet female plug 111, the sheath liquid tube male connector 121 is released, completing the transfer of the sheath liquid tube male connector 121 from the shutdown liquid outlet female plug 112 to the sheath liquid outlet female plug 111.

[0055] The rotation assembly 13 is used to drive the jaw assembly 12 to rotate between the sheath liquid outlet female plug 111 and the shutdown liquid outlet female plug 112. One end of the rotation assembly 13 is connected to the vertical movement assembly 14. This end is used to mount the entire rotation assembly 13 on the vertical movement assembly 14 and does not move on the horizontal plane. The other end of the rotation assembly 13 is connected to the jaw assembly 12, and the rotation assembly 13 drives the jaw assembly 12 to rotate between the sheath liquid outlet female plug 111 and the shutdown liquid outlet female plug 112.

[0056] The vertical movement assembly 14 is used to drive the jaw assembly 12 to move up and down. One end of the vertical movement assembly 14 is connected to the bottom plate 11. This end is used to mount the entire vertical movement assembly 14 on the bottom plate 11. The other end of the vertical movement assembly 14 is connected to the rotation assembly 13, and the vertical movement assembly 14 drives the rotation assembly 13 and the jaw assembly 12 to move up and down together. The vertical movement assembly 14 is used to drive the open jaw assembly 12 to move downward to the same height as the sheath liquid tube male connector 121 or upward away from the bottom plate. It can also drive the jaw assembly 12 holding the sheath liquid tube male connector 121 to move upward away from the bottom plate or downward to connect the sheath liquid tube male connector 121 to the sheath liquid outlet female plug 111 or the shutdown liquid outlet female plug 112.

[0057] When it is determined that the liquid volume in the shutdown liquid barrel reaches the preset value, the automatic switching joint device 10 starts to work. The vertical movement assembly 14 drives the rotation assembly 13 to move upward and maintain a certain height. The rotation assembly 13 drives the jaw assembly 12 to maintain a certain height together, and the jaw assembly 12 maintains this height without contacting the sheath liquid tube male connector 121.

[0058] The rotation assembly 13 drives the jaw assembly 12 to rotate to the sheath liquid tube male connector 121 connected to the sheath liquid outlet female plug 111.

[0059] The jaw assembly 12 remains in the open state. The vertical movement assembly 14 drives the rotation assembly 13 and the jaw assembly 12 to move downward together. The jaw assembly 12 is at the same height as the sheath liquid tube male connector 121, that is, the jaw assembly 12 reaches its gripping surface, and the vertical movement assembly 14 stops operating. The jaw assembly 12 closes and grips the sheath liquid tube male connector 121.

[0060] The vertical motion component 14 drives the rotation component 13 and the jaw component 12 to move upward together. The jaw component 12 holds the sheath fluid tube male connector 121 and leaves the sheath fluid outlet female plug 111. After the sheath fluid tube male connector 121 completely leaves the sheath fluid outlet female plug 111, the vertical motion component 14 stops operating.

[0061] The rotation component 13 drives the jaw component 12 to rotate onto the shutdown fluid outlet female plug 112, and the rotation component 13 stops operating.

[0062] The vertical motion component 14 drives the rotation component 13 and the jaw component 12 to move downward together. The sheath fluid tube male connector 121 held by the jaw component 12 is connected to the shutdown fluid outlet female plug 112, and the vertical motion component 14 stops operating.

[0063] The jaw component 12 opens, releases the sheath fluid tube male connector 121, and the vertical motion component 14 drives the rotation component 13 and the jaw component 12 to move upward together. The jaw component 12 moves away from the sheath fluid tube male connector 121. The connection between the sheath fluid tube male connector 121 and the shutdown fluid outlet female plug 112 is completed.

[0064] As Figure 3 shown, in one embodiment, the rotation component 13 includes a rotating arm 131, a rotating cylinder 132, and a rotating cylinder fixing plate 133. The rotating cylinder fixing plate 133 is arranged on the vertical motion component 14, and the rotating cylinder fixing plate 133 mounts the rotating cylinder 132 on the vertical motion component 14. One end of the rotating arm 131 fixes the rotating cylinder 132, and the other end fixes the jaw component 12. One end of the rotating arm 131 fixed to the rotating cylinder 132 does not move. With the rotating arm 131 as the axis and the rotating cylinder 132 as the center, the rotating arm 131 drives the jaw component 12 to rotate around the rotating cylinder 132. One end of the rotating arm 131 fixing the jaw component 12 can be switched between any position above the sheath fluid outlet female plug 111 or any position above the shutdown fluid outlet female plug 112.

[0065] In one embodiment, the vertical motion component 14 includes: an up-and-down motion cylinder 141 and an up-and-down cylinder fixing plate (not marked in the figure). The up-and-down cylinder fixing plate is fixed on the bottom plate 11, and the up-and-down motion cylinder 141 is fixed on the up-and-down cylinder fixing plate. The up-and-down cylinder fixing plate is used to mount the up-and-down motion cylinder 141 on the bottom plate 11; the up-and-down motion cylinder 141 is used to drive the rotation component 13 and the jaw component 12 to move up and down. The up-and-down motion cylinder 141 drives the gripping end of the jaw component 12 to be at any height, such as higher than the sheath fluid tube male connector 121 or at the same height as the sheath fluid tube male connector 121.

[0066] As Figure 3As shown in FIGS. 4 and 5, in one embodiment, the jaw assembly 12 includes two side jaws: a left jaw 122, a right jaw 123, and a jaw cylinder 124. The left jaw 122 and the right jaw 123 are used to grip or release the sheath fluid tube male connector 121; the jaw cylinder 124 is used to drive the opening and closing of the left jaw 122 and the right jaw 123. When it is necessary to grip the sheath fluid tube male connector 121, the jaw cylinder 124 drives the left jaw 122 and the right jaw 123 to move closer to each other, and the left jaw 122 and the right jaw 123 are closed. When it is necessary to release the sheath fluid tube male connector 121, the jaw cylinder 124 drives the left jaw 122 and the right jaw 123 to separate outward, and the left jaw 122 and the right jaw 123 are opened.

[0067] As Figure 4 shown, in one embodiment, a positioning boss 1101 is provided on the bottom plate 11. The positioning boss 1101 is used to limit the orientation of the joint insertion / removal buttons 1102 of the sheath fluid outlet female plug 111 and the shutdown fluid outlet female plug 112. When the sheath fluid outlet female plug 111 and the shutdown fluid outlet female plug 112 are installed on the bottom plate 11, the positioning boss 1101 limits the joint insertion / removal buttons 1102 of the sheath fluid outlet female plug 111 and the shutdown fluid outlet female plug 112 to face either the outside of the bottom plate or the inside of the bottom plate.

[0068] As Figure 5 shown, in one embodiment, a downward pressure avoidance angle 1301 is provided on the inner side of the bottom of the jaw on the same side as the joint insertion / removal 1103 button. The downward pressure avoidance angle 1301 is used to prevent the joint insertion / removal buttons 1102 of the sheath fluid outlet female plug 111 and the shutdown fluid outlet female plug 112 from being touched when the jaw assembly 13 presses downward.

[0069] In one embodiment, the cleaning effect in the sheath fluid pipeline and the sample pipeline is verified through a cross-contamination rate test or a sterility culture test. In the cross-contamination rate test, a sample with a certain concentration is loaded, and pure water is loaded as a control after performing the cleaning step. The carry-over contamination rate can be obtained by comparing the sample concentration in the pure water group / actual sample concentration. In the sterility culture test, a certain volume of liquid in the sheath fluid pipeline is taken and cultured in a suitable environment, and whether the pipeline is contaminated by bacteria is judged by observing whether there is bacterial activity in the liquid after culture under a microscope.

[0070] In one embodiment, the method judges whether the liquid volume in the shutdown liquid bucket reaches a preset value by judging whether the percentage of the liquid volume in the shutdown liquid bucket in the volume of the shutdown liquid bucket reaches a preset value through a weighing sensor.

[0071] The above are only specific embodiments of the present application, but the protection scope of the present application is not limited thereto. Any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in the present application should be covered within the protection scope of the present application. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.

Claims

1. An automatic switching joint device, characterized in that: Used to automatically switch the sheath fluid tube male connector between the sheath fluid outlet female plug and the shutdown fluid outlet female plug, including: A bottom plate, on which the sheath fluid outlet female plug and the shutdown fluid outlet female plug are installed; A clamping jaw assembly, used for clamping the male connector of the sheath fluid tube; A rotating assembly, used for driving the clamping jaw assembly to rotate between the sheath fluid outlet female plug and the shutdown fluid outlet female plug; The vertical motion assembly is used to drive the clamping jaw assembly to move up and down.

2. The automatic switching joint device according to claim 1, characterized in that: The rotating assembly includes: a rotating arm, a rotating cylinder, and a rotating cylinder fixing plate. The rotary cylinder fixing plate is used to mount the rotary cylinder on the vertical motion assembly; The rotary cylinder is used to drive the rotary arm to rotate with the rotary cylinder as the center; The rotating arm is fixed to the clamping jaw assembly, and drives the clamping jaw assembly to rotate around a circle with the rotating arm as an axis and the rotating cylinder as a center.

3. The automatic switching joint device according to claim 1, characterized in that: The vertical motion assembly includes: an upper and lower motion cylinder, an upper and lower cylinder fixing plate, The upper and lower cylinder fixing plates are used to mount the upper and lower motion cylinders on the bottom plate; The up and down motion cylinder is used to drive the rotating assembly and the clamping jaw assembly to move up and down.

4. The automatic switching joint device according to claim 1, characterized in that: The clamping jaw assembly comprises clamping jaws on both sides and a clamping jaw cylinder. The clamping jaws are used to clamp or release the male connector of the sheath fluid tube; and the clamping jaw cylinder is used to drive the opening and closing of the clamping jaws on both sides.

5. The automatic switching joint device according to claim 1, characterized in that: A positioning boss is arranged on the bottom plate, and the positioning boss is used to limit the orientation of the connector plug-in and pull-out buttons of the sheath liquid outlet female plug and the shutdown liquid outlet female plug.

6. The automatic switching joint device according to claims 4 to 5, characterized in that: A downward pressure avoidance angle is set on the inner side of the bottom of the clamp on the same side as the connector plug-in button, and the downward pressure avoidance angle is used to avoid touching the connector plug-in buttons of the sheath fluid outlet female plug and the shutdown fluid outlet female plug when the clamp assembly is pressed down.

7. A fully automatic shutdown cleaning method for a flow cytometer, characterized in that: include: S1, the flow cytometer receives a shutdown command; S2, the residual sample in the sample chamber is drawn back to the waste liquid bucket, the sample chamber is automatically loaded with a cleaning liquid, and the cleaning liquid flows from the sample chamber to the flow chamber through the sample pipeline; S3, the sheath fluid in the flow chamber flows to the sample chamber through the sample pipeline; S4, determining whether the amount of liquid in the shutdown liquid barrel reaches a preset value; S5, when it is determined that the amount of liquid has not reached the preset value, opening the valve to replenish the shutdown liquid until it reaches the preset value; S6, when it is determined that the amount of liquid reaches a preset value, the automatic connector switching device starts working to transfer the male connector of the sheath liquid tube from the sheath liquid outlet female plug to the shutdown liquid outlet female plug; S7, the shutdown liquid flows from the shutdown liquid barrel to the flow chamber via the sheath liquid pipeline, and the shutdown liquid in the flow chamber is backwashed and rinsed into the sample pipeline; S8, judging the cleaning effect in the sheath fluid pipeline and the sample pipeline; S9, when it is determined that the cleaning effect does not meet the standard, repeating steps S2 to S7 until the cleaning effect meets the standard; S10, when it is determined that the cleaning effect meets the standard, the liquid flow is closed and the machine is automatically shut down; Wherein, the automatic switching joint device is the automatic switching joint device according to any one of claims 1 to claim 6.

8. The fully automatic shutdown cleaning method according to claim 7, characterized in that: The cleaning effect in the sheath fluid pipeline and the sample pipeline is verified by a cross contamination rate test or a sterile culture test.

9. The fully automatic shutdown cleaning method according to claim 7, characterized in that: The method determines whether the amount of liquid in the shutdown liquid barrel reaches a preset value by using a weighing sensor to determine whether the percentage of the liquid volume in the shutdown liquid barrel to the volume of the shutdown liquid barrel reaches a preset value, so as to determine whether the amount of liquid in the shutdown liquid barrel reaches a preset value.

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