Compound pesticide containing kasugamycin and application thereof

Abstract

The invention relates to the field of compound pesticides, in particular to a compound pesticide containing kasugamycin and application of the compound pesticide. The compound pesticide containing kasugamycin is prepared from the following components in percentage by mass: 5 to 10 percent of kasugamycin, 5 to 15 percent of aromatic phenol plant source substances, 8 to 15 percent of myclobutanil and the balance of carriers and / or auxiliaries. The compound pesticide disclosed by the invention can remarkably improve the prevention and treatment effect, has a very good effect in prevention and treatment of rice blast, cucumber angular leaf spot, wheat powdery mildew and watermelon anthracnose, has application universality, and meanwhile, can reduce the dosage and cost of the pesticide and reduce the drug resistance.

Description

Technical Field

[0001] The present invention relates to the technical field of compound pesticides, and specifically relates to a compound pesticide containing kasugamycin. The present invention also relates to the application of the compound pesticide in rice blast, cucumber angular leaf spot, wheat powdery mildew and watermelon anthracnose. Background Art

[0002] In recent years, the occurrence of bacterial and fungal diseases in crops has shown an obvious upward trend, making the disease control more and more difficult. The controlled use of antibiotics has put forward new requirements for the selection of disease control agents, and there is an urgent need for green, safe and highly effective control agents.

[0003] Kasugamycin is a pesticide antibiotic, which is effective against bacteria and some fungi. It mainly inhibits the synthesis of proteins by interfering with the esterase system of the amino acid metabolism of pathogenic bacteria, thereby inhibiting the elongation of hyphae to achieve the bactericidal effect. It has strong systemic and therapeutic effects, but weak protective effects.

[0004] Aromatic phenol plant-derived substances usually have bactericidal and antibacterial effects. Aromatic phenol compounds can destroy the cell membrane of bacteria to play a bactericidal role, and have broad-spectrum properties; moreover, plant-derived substances are extracted from natural plants, are environmentally friendly and have a long action time.

[0005] Myclobutanil mainly acts on crop diseases caused by fungi. Myclobutanil inhibits the biosynthesis of ergosterol in pathogenic bacteria, resulting in the destruction of the structure and function of the cell membrane, thereby achieving the effect of inhibiting fungi.

[0006] The action mechanisms of the above three components are different from each other and are different from traditional insecticides, so there will be no problem of cross-resistance. There are few research reports on the combined pesticide preparations of kasugamycin, aromatic phenol plant-derived substances and myclobutanil. Summary of the Invention

[0007] Aiming at the above technical problems, the first object of the present invention is to provide a compound pesticide containing kasugamycin. By compounding three components of kasugamycin, aromatic phenol plant-derived substances and myclobutanil, the control effect of various plant diseases can be improved.

[0008] The second object of the present invention is to provide the application of the compound pesticide containing kasugamycin in rice blast, cucumber angular leaf spot, wheat powdery mildew and watermelon anthracnose.

[0009] The first object of the present invention is achieved by the following technical solutions: A compound pesticide containing kasugamycin is composed of the following components by mass percentage: kasugamycin 5-10%, aromatic phenol plant-derived substances 5-15%, myclobutanil 8-15%, and the balance is carrier and / or adjuvant.

[0010] According to a preferred embodiment of the present invention, the compound pesticide is composed of the following components by mass percentage: kasugamycin 8-10%, aromatic phenol-based plant-derived substances 5-10%, myclobutanil 10-15%, and the balance is carrier and / or adjuvant.

[0011] According to another preferred embodiment of the present invention, the compound pesticide is composed of the following components by mass percentage: kasugamycin 8%, aromatic phenol-based plant-derived substances 10%, myclobutanil 10%, and the balance is carrier and / or adjuvant.

[0012] According to a preferred embodiment of the present invention, the aromatic phenol-based plant-derived substances include, but are not limited to, one or more of eugenol, thymol, carvacrol, guaiacol, vanillin or protocatechuic acid.

[0013] According to a preferred embodiment of the present invention, the mass ratio of the aromatic phenol-based plant-derived substances to myclobutanil is 1:(1-2).

[0014] According to another preferred embodiment of the present invention, the mass ratio of the aromatic phenol-based plant-derived substances to myclobutanil is 1:1.

[0015] According to a preferred embodiment of the present invention, the adjuvants include, but are not limited to, one or more of polyethylene glycol, glycerol, sodium dodecyl sulfate, sodium dodecylbenzenesulfonate, ammonium sulfate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene phosphate, fatty acid polyoxyethylene ester, benzoic acid, sodium lignosulfonate, carboxymethyl cellulose or polyvinyl alcohol.

[0016] According to a preferred embodiment of the present invention, the carriers include, but are not limited to, one or more of polyglycolic acid, lactic acid-glycolic acid copolymer, polyhydroxybutyrate, polylactide, polycaprolactone, polyorthoester or alkyl cyanoacrylate.

[0017] According to a preferred embodiment of the present invention, the dosage form of the compound pesticide is wettable powder, suspension concentrate, microemulsion or water dispersible granule.

[0018] When preparing the suspension concentrate in the present invention, the carrier or adjuvant examples that can be used may be dispersants selected from sodium dodecylbenzenesulfonate, polycarboxylate or sodium lignosulfonate; wetting agents selected from alkylphenol polyoxyethylene phosphate, phenethylphenol polyoxyethylene phosphate, alkyl sulfates, alkyl sulfonates or naphthalenesulfonates; silicone defoamers; and adhesives selected from polyethylene glycol and glycerol.

[0019] When preparing the wettable powder of the present invention, the carriers or auxiliaries that can be used may be dispersants selected from polycarboxylates, lignosulfonates or alkylnaphthalenesulfonates; wetting agents selected from alkylsulfonates, alkyl sulfates or naphthalenesulfonates; carriers selected from polyglycolic acid, lactic acid-glycolic acid copolymer, polyhydroxybutyric acid, polylactide, polycaprolactone, polyorthoester or alkyl cyanoacrylate.

[0020] When preparing the water dispersible granules of the present invention, the carriers or auxiliaries that can be used may be dispersants selected from polycarboxylates, lignosulfonates or alkylnaphthalenesulfonates; wetting agents selected from alkyl sulfates, polyoxyethylene alcohols, alkylsulfonates or naphthalenesulfonates; binders selected from corn starch, microcrystalline cellulose or diatomite; carriers selected from polyglycolic acid, lactic acid-glycolic acid copolymer, polyhydroxybutyric acid, polylactide, polycaprolactone, polyorthoester or alkyl cyanoacrylate.

[0021] The second object of the present invention is achieved by the following technical solutions: The application of a compound pesticide containing kasugamycin in the prevention and control of rice blast, cucumber angular leaf spot, wheat powdery mildew and watermelon anthracnose.

[0022] Compared with the prior art, the beneficial effects of the present invention are as follows: In the compound pesticide containing kasugamycin of the present invention, the main active ingredients are composed of kasugamycin, aromatic phenol plant-derived substances and myclobutanil. The three have a synergistic effect and have good control effects on various crop diseases. Kasugamycin mainly inhibits the synthesis of proteins by interfering with the esterase system of the amino acid metabolism of pathogenic bacteria, thereby inhibiting the elongation of hyphae to achieve the bactericidal effect. It has strong systemic and therapeutic effects, but weak protectiveness; Aromatic phenol plant-derived substances have bactericidal and antibacterial effects. Aromatic phenol compounds can destroy the cell membrane of bacteria to play a bactericidal role, with broad-spectrum properties. Moreover, the plant-derived substances are extracted from natural plants, are environmentally friendly, have a long action time, and also have antioxidant effects to maintain the timeliness and stability of pesticides; Myclobutanil inhibits the biosynthesis of ergosterol in pathogenic bacteria, resulting in the destruction of the structure and function of the cell membrane, thereby achieving the effect of inhibiting fungi. The combination of the three active components can interact through different mechanisms, reduce drug resistance, and have broad-spectrum properties, improving the protection effect and protection duration, thereby reducing the amount of pesticide used. Specific embodiments

[0023] The following combines specific embodiments to further elaborate on the technical solutions of the present invention in detail, but does not constitute any limitation to the present invention.

[0024] Example 1: 8% kasugamycin, 10% aromatic phenolic plant-derived substances (a 1:1 mixture of guaiacol and vanillin), 15% myclobutanil, 3% polyethylene glycol, 5% sodium lignosulfonate, 3% naphthalene sulfonate, and the balance is lactic acid-glycolic acid copolymer.

[0025] Add each component according to the formula amount to a mixer, stir for 20 minutes, then feed the mixture into a jet mill for ultrafine grinding, and then put it back into the mixer and stir for 30 minutes to make each component evenly distributed.

[0026] Example 2: 10% kasugamycin, 5% aromatic phenolic plant-derived substances (a 1:1 mixture of guaiacol and protocatechuic acid), 8% myclobutanil, 3% polyethylene glycol, 5% sodium lignosulfonate, 3% naphthalene sulfonate, and the balance is lactic acid-glycolic acid copolymer.

[0027] The preparation method is the same as that of Example 1.

[0028] Example 3: 5% kasugamycin, 5% aromatic phenolic plant-derived substances (a 1:1 mixture of guaiacol and vanillin), 10% myclobutanil, 2% sodium lignosulfonate, 1% silicone defoamer, 3% polyethylene glycol, and the balance is water.

[0029] Put kasugamycin, aromatic phenolic plant-derived substances (a 1:1 mixture of guaiacol and vanillin), myclobutanil, sodium lignosulfonate, polyethylene glycol and half of the water into a stone mill according to the formula amount and grind until the particle size is less than 3 microns, then disperse it into a dispersion tank, add the remaining water and defoamer, and stir at high speed for 30 minutes.

[0030] Example 4: 8% kasugamycin, 10% aromatic phenolic plant-derived substances (a 1:1 mixture of guaiacol and vanillin), 10% myclobutanil, 2% sodium lignosulfonate, 1% silicone defoamer, 3% polyethylene glycol, and the balance is water.

[0031] The preparation method is the same as that of Example 3.

[0032] Comparative Example 1: A pesticide without kasugamycin 10% aromatic phenolic plant-derived substances (a 1:1 mixture of guaiacol and vanillin), 15% myclobutanil, 3% polyethylene glycol, 5% sodium lignosulfonate, 3% naphthalene sulfonate, and the balance is lactic acid-glycolic acid copolymer.

[0033] Other steps are the same as those of Example 1.

[0034] Comparative Example 2: A pesticide without aromatic phenolic plant-derived substances 8% kasugamycin, 15% myclobutanil, 3% polyethylene glycol, 5% sodium lignosulfonate, 3% naphthalene sulfonate, and the balance is lactic acid-glycolic acid copolymer.

[0035] The preparation method is the same as that of Example 1.

[0036] Comparative Example 3, a pesticide without myclobutanil Kasugamycin 8%, aromatic phenol plant-derived substances (guaiacol and vanillin mixed at 1:1) 10%, polyethylene glycol 3%, sodium lignosulfonate 5%, naphthalene sulfonate 3%, and the balance is lactic acid-glycolic acid copolymer.

[0037] Other steps are the same as those of Example 1.

[0038] The above pesticide formulations were subjected to field efficacy tests against rice blast, cucumber angular leaf spot, wheat powdery mildew, and watermelon anthracnose.

[0039] Experimental Example 1, field control efficacy experiment against rice blast Test method: The test area is 66.7 m for each plot 2 , repeated 3 times; the survey method before and after spraying is as follows: Randomly sample 5 points in the experimental treatment area, grade the disease according to the relevant national field test standards, and calculate the control efficacy.

[0040] The test was carried out at the rice test base in Fuyang District, Zhejiang Province. The test variety was a hybrid rice variety. It was uniformly sprayed by diluting with water at 3 g of active ingredient per mu. A total of 1 spraying was carried out. The control results at 7 days, 14 days, and 21 days after spraying are shown in Table 1.

[0041] Table 1 Statistical table of field efficacy test results of rice blast

[0042] As can be seen from Table 1, the compound pesticide of the embodiment of the present invention has better control efficacy against rice blast at a relatively low dosage than the control effects of Comparative Examples 1 to 3, indicating that the compound pesticide shows excellent synergistic effect compared with the single agents of each component and has a long-lasting effect period.

[0043] Experimental Example 2, field control efficacy experiment against wheat powdery mildew Test method: The test area is 66.7 m for each plot 2 , repeated 3 times; the survey method before and after spraying is as follows: Randomly sample 5 points in the experimental treatment area, grade the disease according to the relevant national field test standards, and calculate the control efficacy.

[0044] The test was carried out at the wheat planting base in Shidaofan, Changshan Township, Zhejiang Province. It was uniformly sprayed by diluting with water at 3 g of active ingredient per mu. A total of 1 spraying was carried out. The control results at 7 days, 14 days, and 21 days after spraying are shown in Table 2.

[0045] Table 2 Statistical table of field efficacy test results of wheat powdery mildew

[0046] As can be seen from Table 2, the compound pesticide of the embodiment of the present invention has a better control effect on wheat powdery mildew at a relatively low dosage than that of Comparative Examples 1 to 3. The compound pesticide shows excellent synergistic effect and a long effective period compared with the single agents of each component.

[0047] Experimental Example 3, Field Control Effect Experiment of Controlling Cucumber Angular Leaf Spot Test method: The test area is 66.7 m for each plot 2 , with 3 repetitions; the investigation method before and after spraying is as follows: randomly sample 5 points in the experimental treatment area, grade the disease according to the relevant national field test standards, and calculate the control effect.

[0048] The test was carried out in the vegetable planting base of Dongtang Village, Jingnan Township, Jingning, Lishui, Zhejiang Province. Uniform spraying was carried out by diluting with water at 3 g of active ingredient per mu. A total of 1 spraying was carried out. The control results at 7 days, 14 days and 21 days after spraying are shown in Table 3.

[0049] Table 3 Statistical Table of Field Efficacy Test Results of Cucumber Angular Leaf Spot

[0050] As can be seen from Table 3, the compound pesticide of the embodiment of the present invention has a better control effect on cucumber angular leaf spot at a relatively low dosage than that of Comparative Examples 1 to 3. The compound pesticide shows excellent synergistic effect and a long effective period compared with the single agents of each component.

[0051] Experimental Example 4, Field Control Effect Experiment of Controlling Watermelon Anthracnose Test method: The test area is 66.7 m for each plot 2 , with 3 repetitions; the investigation method before and after spraying is as follows: randomly sample 5 points in the experimental treatment area, grade the disease according to the relevant national field test standards, and calculate the control effect.

[0052] The test was carried out in the watermelon planting base of Gongchengshan Village, Anhua Town, Zhuji, Zhejiang Province. Uniform spraying was carried out by diluting with water at 3 g of active ingredient per mu. A total of 1 spraying was carried out. The control results at 7 days, 14 days and 21 days after spraying are shown in Table 4.

[0053] Table 4 Statistical Table of Field Efficacy Test Results of Rice Blast

[0054] As can be seen from Table 4, the compound pesticide of the embodiment of the present invention has a better control effect on watermelon anthracnose at a relatively low dosage than that of Comparative Examples 1 to 3. The compound pesticide shows excellent synergistic effect and a long effective period compared with the single agents of each component.

[0055] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principle of the present invention shall be equivalent replacement methods and are all included in the protection scope of the present invention.

Claims

1. A compound pesticide containing kasugamycin, characterized in that, It is composed of the following components by mass percentage: kasugamycin 5-10%, aromatic phenol plant-derived substances 5-15%, myclobutanil 8-15%, and the balance is carrier and / or adjuvant.

2. The compound pesticide containing kasugamycin according to claim 1, characterized in that, It is composed of the following components by mass percentage: kasugamycin 8-10%, aromatic phenol plant-derived substances 5-10%, myclobutanil 10-15%, and the balance is carrier and / or adjuvant.

3. The compound pesticide containing kasugamycin according to claim 1 or 2, characterized in that, It is composed of the following components by mass percentage: kasugamycin 8%, aromatic phenol plant-derived substances 10%, myclobutanil 10%, and the balance is carrier and / or adjuvant.

4. The compound pesticide containing kasugamycin according to claim 1, characterized in that, The aromatic phenol plant-derived substances include, but are not limited to, one or more of eugenol, thymol, carvacrol, guaiacol, vanillin, or protocatechuic acid.

5. The compound pesticide containing kasugamycin according to claim 1, characterized in that, The mass ratio of the aromatic phenol plant-derived substances to myclobutanil is 1:(1-2).

6. The compound pesticide containing kasugamycin according to claim 5, characterized in that, The mass ratio of the aromatic phenol plant-derived substances to myclobutanil is 1:

1.

7. The compound pesticide containing kasugamycin according to claim 1, characterized in that, The adjuvants include, but are not limited to, one or more of polyethylene glycol, glycerol, sodium dodecyl sulfate, sodium dodecylbenzenesulfonate, ammonium sulfate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether phosphate, fatty acid polyoxyethylene ester, benzoic acid, sodium lignosulfonate, carboxymethyl cellulose, or polyvinyl alcohol.

8. The compound pesticide containing kasugamycin according to claim 1, characterized in that, The carriers include, but are not limited to, one or more of polyglycolic acid, lactic acid-glycolic acid copolymer, polyhydroxybutyrate, polylactide, polycaprolactone, polyorthoester, or alkyl cyanoacrylate.

9. The compound pesticide containing kasugamycin according to claim 1, characterized in that, The dosage form of the compound pesticide is wettable powder, suspension concentrate, microemulsion, or water dispersible granule.

10. Application of the compound pesticide containing kasugamycin according to claim 1 in controlling rice blast, cucumber angular leaf spot, wheat powdery mildew and watermelon anthracnose.

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