Dynamic incubator and incubation equipment

Abstract

The invention provides a dynamic incubator and incubation equipment. The dynamic incubator comprises a shell mechanism, a driving mechanism and an incubation mechanism, a mounting cavity is formed in the shell mechanism; the driving mechanism comprises a driving piece and a driven piece, and the driving piece is in driving connection with the driven piece; the incubation mechanism comprises a supporting assembly and an incubation assembly, and the supporting assembly is connected with the inner wall of the mounting cavity; the supporting assembly comprises an incubation pot provided with an incubation groove, and the incubation assembly is detachably arranged in the incubation groove; the driven part is in transmission connection with the incubation assembly, and the incubation assembly comprises a cover plate and a slide glass; and the driving piece can drive the driven piece to move so as to drive the cover plate to move relative to the slide glass. Therefore, the reagent between the cover plate and the slide glass can be uniformly mixed, so that the reagent fully infiltrates a tissue sample on the slide glass, a detected object in the reagent and a detected object in the sample are combined more quickly and uniformly, and the efficiency and the quality of treating the sample on the slide glass are further improved.

Description

Technical Field

[0001] The present disclosure relates to the technical field of biological sample processing, and particularly to a dynamic incubator and an incubation device. Background Art

[0002] The detection of tissue morphology, proteins, pathogens or genes on slide specimens (such as HE staining, immunohistochemical staining, special staining, Papanicolaou staining, HPV fractal and detection of cervical cell smears, in-situ hybridization, etc.) is a necessary means for clinical disease diagnosis. However, the whole detection process has complex steps. Simplifying the operation process and reducing the influence of environmental factors are the top priorities for improving the detection efficiency and accuracy of specimens.

[0003] Generally, in the middle of the specimen processing process, the vast majority of specimens need antigen retrieval, which is a necessary step to make protein or gene detection reach the required sensitivity and specificity. It is generally carried out at a temperature of 80 - 121°C and maintained for 3 - 60 minutes. The most commonly used methods are pressure cooker cooking, ordinary boiling, microwave cooking, hot water bath heating or baking, etc. Then, reagents are added dropwise for staining or detection processing.

[0004] In the related art, for the common processing of slide specimens, the slide specimens are placed in an open container for antigen retrieval, staining or detection. However, during staining or detection, it is mostly static incubation. Static incubation treatment is to drop the reagent onto the slide specimen and let it stand for incubation for a period of time. This static incubation method takes a long time, and the binding speed between the detected substance in the reagent and the substance to be detected in the specimen is slow and uneven, which is time-consuming and laborious, and seriously affects the detection quality and efficiency.

[0005] It should be noted that the information disclosed in the above background art section is only used to enhance the understanding of the background of the present disclosure, and thus may include information that does not constitute the prior art known to those of ordinary skill in the art. Summary of the Invention

[0006] The purpose of the present disclosure is to provide a dynamic incubator and an incubation device, which can at least overcome to a certain extent the problems of long time required for static incubation of tissue specimens and low detection repeatability in the related art.

[0007] Other characteristics and advantages of the present disclosure will become apparent through the following detailed description, or will be learned in part through the practice of the present disclosure.

[0008] According to one aspect of the present disclosure, a dynamic incubator is provided, which includes a housing mechanism, a driving mechanism, and an incubation mechanism; an installation cavity is provided inside the housing mechanism; the driving mechanism includes a driving member and a driven member, and the driving member is drivingly connected to the driven member; the incubation mechanism includes a support assembly and an incubation assembly, and the support assembly is connected to the inner wall of the installation cavity; the support assembly includes an incubation pot provided with an incubation groove, and the incubation assembly is detachably arranged in the incubation groove; the driven member is drivingly connected to the incubation assembly, and the incubation assembly includes a cover plate and a carrier sheet; the driving member can drive the driven member to move, so as to drive the cover plate to move relative to the carrier sheet.

[0009] By drivingly connecting the driven member to the incubation assembly, and the driving member can drive the driven member to move, and drive the cover plate in the incubation assembly to move relative to the carrier sheet, so as to mix the reagent between the cover plate and the carrier sheet, make the reagent fully infiltrate the tissue sample on the carrier sheet, and make the binding of the detected substance in the reagent and the substance to be detected in the sample faster and more uniform, thereby improving the efficiency and quality of specimen processing on the carrier sheet.

[0010] In an embodiment of the present disclosure, the driving member is connected to the housing mechanism and is located below the incubation mechanism, and the driving member is within the range of the top view projection of the incubation mechanism; the driven member is hinged to the housing mechanism, and the lower end of the driven member is hinged to the output end of the driving member, and the driving member can drive the upper end of the driven member to swing back and forth.

[0011] By arranging the driving member below the incubation mechanism and within the range of the top view projection of the incubation mechanism, the lower end of the driven member is connected to the output end of the driving member, and the upper end of the driven member can swing back and forth, so that the incubation mechanism, the driven member, and the driving member are arranged in a U shape, which can reduce the front-to-back distance of the housing mechanism and its floor area, and avoid the linear arrangement of the incubation mechanism, the driven member, and the driving member, resulting in a large front-to-back distance of the housing mechanism and occupying space.

[0012] In an embodiment of the present disclosure, the support assembly is movably connected to the inner wall of the installation cavity in a push-pull manner.

[0013] By arranging the support assembly and the inner wall of the installation cavity to be movably connected in a push-pull manner, the support assembly has the functions of being pulled open and closed relative to the housing mechanism, and the incubation assembly and the incubation groove are detachably connected, which is convenient for the disassembly and assembly of the incubation assembly and the incubation pot.

[0014] In an embodiment of the present disclosure, the support assembly further includes a support frame and a pair of slide rails, and the support frame is slidably connected to the side wall of the installation cavity through the pair of slide rails; the incubation pot is connected to the support frame, and the incubation pot can drive the incubation assembly to move back and forth.

[0015] The support frame is slidably connected to the side wall of the installation cavity through a pair of sliding rails, and the incubation pot is connected to the support frame, so that the support frame can drive the incubation pot to slide out of the installation cavity, and then drive the incubation assembly to move back and forth, facilitating the removal of the incubation assembly from the incubation tank, and facilitating the loading of tissue samples and the cleaning of the incubation assembly.

[0016] In an embodiment of the present disclosure, the support assembly further includes a connection assembly, and the connection assembly includes a first push plate, a second push plate, a connecting rod and an elastic member. The first push plate and the second push plate are respectively connected to two ends of the connecting rod. The connecting rod passes through the incubation pot, and the elastic member is sleeved on the connecting rod and located between the incubation pot and the first push plate; the driven member can abut against the first push plate, and the second push plate is drivingly connected to the cover plate.

[0017] By arranging the elastic member on the connecting rod and between the incubation pot and the first push plate, and the first push plate and the second push plate are respectively connected to two ends of the connecting rod, the synchronous movement of the first push plate and the second push plate can be realized, and the function of restoring to the original state is provided. When the upper end of the driven member swings forward, the driven member can press the first push plate forward to drive the second push plate to move forward and the elastic member is compressed. When the upper end of the driven member swings backward, the elastic member has a tendency to return to its original state to drive the first push plate and the second push plate to return to the position of the original state. The driven member can abut against the first push plate, that is, the connection assembly and the drive assembly are detachably connected. When the support assembly is pulled out of the installation cavity, the drive assembly will not move forward with the connection assembly, avoiding damage to the wires on the drive assembly due to being pulled.

[0018] In an embodiment of the present disclosure, the support assembly includes at least two incubation pots, and different incubation pots are arranged side by side.

[0019] By providing at least two incubation pots, each incubation pot can load an incubation assembly, so that multiple incubation assemblies can be operated at one time, saving time and improving the detection efficiency. At the same time, detection can also be carried out in batches, with one incubation pot as one batch, so that tissue samples of different batches can be added for detection at different time points during the detection process.

[0020] In an embodiment of the present disclosure, a limiting post is provided at the bottom of the incubation tank, and a limiting groove is provided at the bottom of the incubation assembly. The limiting post can cooperate with the limiting groove to limit the shaking of the incubation assembly.

[0021] By the cooperation of the provided limiting post and the limiting groove, the position of the incubation assembly in the incubation tank can be fixed.

[0022] In one embodiment of the present disclosure, the support assembly further includes a heating element and a heat insulation element. The heating element is connected to the bottom of the incubation pot, and the heat insulation element is disposed below the heating element and connected to the support frame.

[0023] By connecting the heating element to the bottom of the incubation pot, the heat conduction performance can be better. The heat insulation element is disposed below the heating element and connected to the support frame, which can play a role in heat insulation and protection.

[0024] In one embodiment of the present disclosure, the housing mechanism includes an upper cover disposed above the incubation pot and capable of opening and closing. A plurality of liquid dropping holes are formed in the upper cover, and the plurality of liquid dropping holes correspond to the plurality of carriers one by one. A reagent can pass through the liquid dropping holes and flow between the carrier and the cover plate.

[0025] By providing an upper cover capable of opening and closing above the incubation pot, a plurality of liquid dropping holes corresponding to the plurality of carriers one by one are formed in the upper cover, and the reagent can pass through the liquid dropping holes and flow between the carrier and the cover plate, which facilitates the dropping of the reagent. The upper cover can open and close. When the upper cover is opened, it is convenient to take out the incubation assembly from the incubation tank. When the upper cover is closed, foreign objects can be prevented from falling into the incubation tank and contaminating the tissue sample in the incubation assembly.

[0026] According to still another aspect of the present disclosure, there is provided an incubation device, including an automatic reagent dropping device and the above-mentioned dynamic incubation box. The automatic reagent dropping device can drop the reagent between the cover plate and the carrier.

[0027] Through the cooperation of the automatic reagent dropping device and the dynamic incubation box, the dynamic incubation of the tissue sample can be made more convenient and the manual operation can be reduced.

[0028] It should be understood that the above general description and the following detailed description are only exemplary and explanatory, and cannot limit the present disclosure. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] The accompanying drawings herein are incorporated into the specification and form a part of the specification, showing embodiments consistent with the present disclosure, and are used together with the specification to explain the principles of the present disclosure. Obviously, the accompanying drawings in the following description are only some embodiments of the present disclosure. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0030] Figure 1 Showing a schematic structural diagram of a dynamic incubation box in an embodiment of the present disclosure.

[0031] Figure 2 is Figure 1 A schematic structural diagram of the incubation mechanism of the dynamic incubation box in the pulled-open state and the upper cover opened state.

[0032] Figure 3 Is Figure 2 A schematic structural view of the incubation component separated from the incubation mechanism in

[0033] Figure 4 Is Figure 2 A schematic structural view of the housing mechanism and the driving mechanism in

[0034] Figure 5 Is Figure 1 A schematic cross-sectional view of the dynamic incubation box in the first perspective in

[0035] Figure 6 Is Figure 1 A schematic exploded view of the support component of the incubation mechanism in

[0036] Figure 7 Is Figure 1 A schematic structural view of the support component and the incubation component in

[0037] Figure 8 Is Figure 1 A schematic structural view of the incubation component in

[0038] Figure 9 Is Figure 1 The first schematic structural view of the dynamic incubation box after removing the rear plate in

[0039] Figure 10 Is Figure 1 A schematic cross-sectional view of the dynamic incubation box in the second perspective in

[0040] Figure 11 Is Figure 1 The second schematic structural view of the dynamic incubation box after removing the rear plate in

[0041] Figure 12 Shows a schematic structural view of placing a dynamic incubation box on an incubation device in an embodiment of the present disclosure.

[0042] Among them, the reference numerals are explained as follows:

[0043] 1. Dynamic incubation box; 2. Automatic reagent dropping device;

[0044] 100. Housing mechanism; 110. Front plate; 120. Rear plate; 130. First side plate; 140. Second side plate; 150. Upper cover; 151. Drip hole; 160. Base plate; 161. Fan; 170. Mounting plate;

[0045] 200. Driving mechanism; 210. Driving member; 220. Driven member;

[0046] 300. Incubation mechanism; 310. Support component; 311. Incubation pot; 312. Incubation tank; 313. Limit post; 314. Link hole; 315. Support frame; 316. First support plate; 317. Second support plate; 318. Third support plate; 319. Cross beam; 320. Front panel; 321. First slide rail; 322. Second slide rail; 323. Heating element; 324. Heat insulation component; 330. Connection component; 331. First push plate; 332. Second push plate; 333. Connecting rod; 334. Elastic component; 335. Water inlet hole; 336. Water inlet joint; 337. Water outlet hole; 338. Water outlet joint; 340. Incubation component; 341. Bracket; 342. Push-pull component; 343. Cover plate; 344. Bottom plate; 345. Slide; 346. Limit groove. Detailed implementation manners

[0047] Example embodiments will now be described more fully with reference to the accompanying drawings. However, the example embodiments can be implemented in various forms and should not be construed as limited to the examples set forth herein; rather, these embodiments are provided so that this disclosure will be more complete and comprehensive, and will fully convey the concept of the example embodiments to those skilled in the art. The described features, structures, or characteristics can be combined in any suitable manner in one or more embodiments.

[0048] In addition, the accompanying drawings are only schematic illustrations of the present disclosure and are not necessarily drawn to scale. The same reference numerals in the drawings denote the same or similar parts, and thus repeated descriptions thereof will be omitted. Some of the block diagrams shown in the drawings are functional entities and do not necessarily correspond to physically or logically independent entities. These functional entities can be implemented in software form, or in one or more hardware modules or integrated circuits, or in different networks and / or processor devices and / or microcontroller devices.

[0049] Referring to Figures 1 to 4 , in this embodiment, a dynamic incubator is provided. The dynamic incubator 1 includes a housing mechanism 100, a driving mechanism 200, and an incubation mechanism 300. An installation cavity is provided in the housing mechanism 100. The driving mechanism 200 and the incubation mechanism 300 are arranged in the installation cavity. The driving mechanism 200 can drive the cover plate 343 in the incubation mechanism 300 to move relative to the slide 345, so as to mix the reagent between the cover plate 343 and the slide 345, make the reagent fully infiltrate the tissue sample on the slide 345, and enable the combination of the detected substance in the reagent and the substance to be detected in the sample to be faster and more uniform, realizing dynamic incubation, and thus improving the efficiency and quality of specimen processing on the slide 345.

[0050] Among them, the driving mechanism 200 includes a driving member 210 and a driven member 220, and the driving member 210 is drivingly connected to the driven member 220. The incubation mechanism 300 includes a support assembly 310 and an incubation assembly 340, and the support assembly 310 is connected to the inner wall of the installation cavity. The support assembly 310 includes an incubation pot 311 provided with an incubation groove 312, and the incubation assembly 340 is detachably arranged in the incubation groove 312. The driven member 220 is drivingly connected to the incubation assembly 340. The incubation assembly 340 includes a cover plate 343 and a slide 345. The driving member 210 can drive the driven member 220 to move, so as to drive the cover plate 343 to move relative to the slide 345.

[0051] In this embodiment, by drivingly connecting the driven member 220 with the incubation assembly 340, and the driving member 210 can drive the driven member 220 to move, and drive the cover plate 343 in the incubation assembly 340 to move relative to the slide 345, so as to mix the reagent between the cover plate 343 and the slide 345, make the reagent fully infiltrate the tissue sample on the slide 345, and make the binding of the detected substance in the reagent and the substance to be detected in the sample faster and more uniform, thereby improving the efficiency and quality of specimen processing on the slide 345 and avoiding the problem of low detection repeatability.

[0052] Reference Figures 2 to 4 , the housing mechanism 100 may include a front plate 110, a rear plate 120, a first side plate 130, a second side plate 140, an upper cover 150 and a base plate 160. An installation cavity is formed by connecting the front plate 110, the rear plate 120, the first side plate 130, the second side plate 140, the upper cover 150 and the base plate 160. This installation cavity can be used to install the incubation mechanism 300, the driving mechanism 200, the fan 161, etc.

[0053] The first side plate 130 is arranged on the right side, the second side plate 140 is arranged on the left side, and the right edge of the upper cover 150 is movably connected to the upper edge of the first side plate 130, so that the upper cover 150 can take the upper edge of the first side plate 130 as an axis and make an opening and closing movement relative to the second side plate 140. For example, the connection between the upper cover 150 and the first side plate 130 can be realized through a connecting rod and a bearing, so as to achieve the flip connection between the upper cover 150 and the first side plate 130. In this way, the upper cover 150 can be opened and closed. When the upper cover 150 is opened, it is convenient to take out the incubation assembly 340 from the incubation groove 312. When the upper cover 150 is closed, it can prevent foreign objects from falling into the incubation groove 312 and polluting the tissue sample in the incubation assembly 340.

[0054] It should be noted that the connection method between the upper cover 150 and the first side plate 130 is not limited to the flip connection, and can also be a fixed connection. For example, one edge of the upper cover 150 is fixedly connected to the first side plate 130, and the upper cover 150 can be the same as the structure of a shutter, with the functions of opening and closing, so that the opening and closing of the upper cover 150 can also be realized.

[0055] A fan 161 is installed on the base plate 160. The fan 161 is located below the incubation mechanism 300 and in front of the driving member 210. When cooling is required inside the housing mechanism 100, it can be achieved through the fan 161. In addition, heat dissipation holes can also be provided on the base plate 160 and the rear plate 120 for cooling to protect the electronic components inside the housing structure.

[0056] Reference Figure 8 , in an embodiment, a plurality of liquid dripping holes 151 are provided on the upper cover 150. The liquid dripping holes 151 are through holes penetrating the upper surface and the lower surface of the upper cover 150, and the upper cover 150 is located above the incubation pot 311. Among them, the incubation pot 311 can hold the incubation component 340. The incubation component 340 includes a cover plate 343 and a carrier sheet 345. The plurality of liquid dripping holes 151 correspond one-to-one to the plurality of carrier sheets 345 below, and the lower part of the carrier sheet 345 is aligned directly below the liquid dripping holes 151. When the reagent passes through the liquid dripping holes 151, it can flow from the upper part of the carrier sheet 345 to between the carrier sheet 345 and the cover plate 343. It should be noted that the upper cover 150 may not be provided with liquid dripping holes 151. When adding the reagent, the upper cover 150 can be opened and the reagent can be dropped between the carrier sheet 345 and the cover plate 343.

[0057] Reference Figure 11 , grooves can be provided on the first side plate 130 and the second side plate 140. The grooves on both sides can be symmetrically arranged. The grooves are rectangular, which can facilitate the operator to pick up the dynamic incubator 1 for movement. It should be noted that the grooves are not limited to rectangles and can also be oval, inverted triangle, square, etc.

[0058] Reference Figures 3 to 6 , the incubation mechanism 300 can include a support component 310 and an incubation component 340. The support component 310 is movably connected to the inner wall of the installation cavity in a push-pull manner. The incubation component 340 can be placed in the support component 310, and the support component 310 can drive the incubation component 340 to move back and forth. Among them, an opening can be provided on the front plate 110 of the housing mechanism 100 for avoiding the back-and-forth movement of the incubation mechanism 300.

[0059] By setting the support component 310 to be movably connected to the inner wall of the installation cavity in a push-pull manner, the support component 310 has the functions of being pulled open and closed relative to the housing mechanism 100, that is, the support component 310 can be installed inside the housing mechanism 100 like a drawer, and the incubation component 340 is detachably connected to the incubation tank 312, which facilitates the disassembly and assembly of the incubation component 340 and the incubation pot 311.

[0060] In one embodiment, the support assembly 310 may include a support frame 315, a pair of slide rails, and an incubation pot 311. The pair of slide rails are respectively connected to both sides of the support frame 315, and the incubation pot 311 is connected to the support frame 315. An incubation groove 312 is formed in the incubation pot 311, and the incubation groove 312 can accommodate the incubation assembly 340.

[0061] The support frame 315 may include a first support plate 316, a second support plate 317, a third support plate 318, and a cross beam 319. The third support plate 318 is disposed in front of the cross beam 319, and both ends of the third support plate 318 are respectively connected to the front ends of the first support plate 316 and the second support plate 317. Both ends of the cross beam 319 are respectively connected to the rear ends of the first support plate 316 and the second support plate 317, and the cross beam 319 is used to support the bottom of the incubation pot 311.

[0062] Wherein, the connection manner between the incubation pot 311 and the support frame 315 may be a fixed connection or a support connection. For example, the four sides of the incubation pot 311 can be respectively fixedly connected to the first support plate 316, the second support plate 317, the third support plate 318, and the cross beam 319 through nuts and bolts. Another example is that step grooves are formed on the upper edges of the first support plate 316, the second support plate 317, and the third support plate 318, so that the upper edge of the pot body of the incubation pot 311 can be supported in the step grooves, and the bottom of the incubation pot 311 is supported on the cross beam 319, which can be more convenient when the incubation pot 311 needs to be replaced.

[0063] The pair of slide rails may include a first slide rail 321 and a second slide rail 322. The first support plate 316 and the first side plate 130 are connected to both sides of the first slide rail 321, and the second support plate 317 and the second side plate 140 are connected to both sides of the second slide rail 322. By pulling the third support plate 318 to move back and forth, the support frame 315 can move back and forth under the guidance of the first slide rail 321 and the second slide rail 322.

[0064] In this embodiment, the support frame 315 is slidably connected to the side wall of the installation cavity through a pair of slide rails, and the incubation pot 311 is connected to the support frame 315. In this way, the support frame 315 can drive the incubation pot 311 to slide out of the installation cavity, and then drive the incubation assembly 340 to move back and forth, which is convenient for taking out the incubation assembly 340 from the incubation groove 312, and is convenient for loading tissue samples and cleaning the incubation assembly 340.

[0065] It should be noted that in this embodiment, the support component 310 is movably connected to the inner wall of the installation cavity in a push-pull manner, but the present disclosure is not limited thereto. For example, the support component 310 and the inner wall of the installation cavity may be fixedly connected. Among them, the support component 310 may include a support frame 315 and an incubation pot 311. The support frame 315 is fixedly connected to the inner walls on both sides of the installation cavity, and the incubation pot 311 is connected to the support frame 315. The upper cover 150 of the housing mechanism 100 is flip-connectable to the first side plate 130, and the upper cover 150 can move in an opening and closing manner relative to the second side plate 140. In the open state of the upper cover 150, it is convenient to take out the incubation component 340 from the incubation pot 311.

[0066] In one embodiment, the support component 310 may further include a front panel 320. A display screen may be provided on the front panel 320. The display screen may be a touch screen, which can not only display the working state information of the dynamic incubator 1, but also perform control operations on the display screen. It should be noted that the display screen is a prior art and will not be elaborated herein. A start button may also be provided beside the display screen for controlling the start and stop of the dynamic incubator 1.

[0067] In one embodiment, the support component 310 may further include a heating element 323 and a heat insulation member 324. The heating element 323 is connected to the bottom of the incubation pot 311 and fixedly connected to the support frame 315. The heating element 323 is used to heat the pot body of the incubation pot 311, and thus can heat the water in the incubation tank 312. The heat insulation member 324 is disposed below the heating element 323 and connected to the bottom of the support frame 315. The heat insulation plate mainly plays a role of heat insulation protection to prevent other electronic components in the installation cavity from being damaged due to excessive temperature during the heating process of the heating element 323. It should be noted that the heating element 323 may be a heating sheet, a heating film or other heating devices, and the heat insulation member 324 may be a heat insulation plate.

[0068] Reference Figures 3 to 6 , the support component 310 includes at least two incubation pots 311, and different incubation pots 311 are arranged side by side. At least one incubation component 340 can be loaded in each incubation tank 312.

[0069] In one embodiment, two incubation pots 311 are connected to the support frame 315. An incubation tank 312 is provided on each incubation pot 311 for carrying the incubation component 340. Among them, the left incubation pot 311 can carry 3 incubation components 340, and the right incubation pot 311 can carry 2 incubation components 340. In this way, 5 incubation components 340 can be operated at one time, saving time and improving the detection efficiency. At the same time, the detection can also be carried out in batches, with one incubation pot 311 or one incubation component 340 as one batch. In this way, tissue samples of different batches can be added at different time points during the detection process for detection.

[0070] Although in the above embodiments, two incubation pots 311 are connected to the support frame 315, the present disclosure is not limited thereto. For example, it can be expanded according to the actual application situation, and one, three, four, or five incubation pots 311 can be provided on the support frame 315. Among them, the specifications of the incubation pots 311 can be selected, and there are incubation pots 311 that can hold one, two, three, four, or five incubation components 340.

[0071] Reference Figures 6 to 8 , a limiting post 313 is provided at the bottom of the incubation tank 312, and a limiting groove 346 is provided at the bottom of the incubation component 340. The limiting post 313 can cooperate with the limiting groove 346 to limit the shaking of the incubation component 340.

[0072] Among them, the incubation component 340 includes a bracket 341, a push-pull component 342, a cover plate 343, a bottom plate 344, and a carrier sheet 345. Multiple groups of cover plates 343 and bottom plates 344 are connected to the bracket 341. A carrier sheet 345 can be placed between each group of cover plates 343 and bottom plates 344. By the forward and backward pushing of the push-pull component 342, the cover plate 343 can move relative to the carrier sheet 345, so that the reagent between the cover plate 343 and the carrier sheet 345 can be stirred, and the reaction between the reagent and the tissue sample on the carrier sheet 345 can be accelerated. It should be noted that this incubation component 340 is a prior art and will not be elaborated here.

[0073] In one embodiment, limiting grooves 346 are opened at the bottoms on both sides of the bracket 341. Two limiting grooves 346 can be opened on each side of the bracket 341, and the limiting grooves 346 are semi-cylindrical. Correspondingly, a plurality of limiting posts 313 are provided at the bottom of the incubation tank 312. The limiting posts 313 can correspond to the limiting grooves 346 one by one. The limiting grooves 346 are adapted to the limiting posts 313. The limiting posts 313 can be inserted into the limiting grooves 346, and the forward and backward and left and right shaking of the incubation component 340 can be limited by two or more limiting posts 313. Among them, in the left-to-right direction, the distance between two adjacent limiting posts 313, or the distance between the limiting post 313 and the side of the incubation tank 312 is slightly greater than the width of the incubation component 340. In the front-to-back direction, the distance between two adjacent limiting posts 313 is equal to the distance between two limiting grooves 346 on the same side of the incubation component 340.

[0074] Reference Figure 7 and Figure 9In one embodiment, a water inlet hole 335 is provided on the rear side wall of the incubation tank 312, and a water inlet joint 336 is provided on the rear side of the incubation pot 311. The water inlet hole 335 is connected to the water inlet joint 336, and the water inlet joint 336 is connected to the water inlet pump. A water outlet hole 337 is provided on the bottom of the incubation tank 312, and a water outlet joint 338 is provided on the bottom of the incubation pot 311. The water outlet hole 337 is connected to the water outlet joint 338, and the water outlet joint 338 is connected to the water outlet pump.

[0075] refer to Figure 6 and Figure 7 The driving mechanism 200 is connected to the incubation component 340 through the driving member 210 to drive the driven member 220 to move, thereby driving the cover plate 343 to move relative to the carrier 345.

[0076] In one embodiment, the support assembly 310 further includes a connecting assembly 330, which includes a first push plate 331, a second push plate 332, a connecting rod 333 and an elastic member 334. The second push plate 332 may be a plate-shaped object made of a magnet, the rear end of the push-pull assembly 342 may be made of a material that can be adsorbed by a magnet, such as iron, nickel, cobalt, etc., and the elastic member 334 may be a spring, etc.

[0077] The first push plate 331 and the second push plate 332 are connected to the two ends of the connecting rod 333, respectively. A connecting rod hole 314 is provided at the rear side of the incubation pot 311 and at a position corresponding to the second push plate 332. The connecting rod hole 314 is a circular through hole, and the connecting rod 333 can pass through the connecting rod hole 314 and move forward and backward in the connecting rod hole 314. The first push plate 331 and the second push plate 332 are connected to the two ends of the connecting rod 333, respectively. The second push plate 332 is located in the incubation tank 312. The elastic member 334 is sleeved on the connecting rod 333 and is located between the incubation pot 311 and the first push plate 331.

[0078] By sleeve-arranging the elastic member 334 on the connecting rod 333 and being located between the incubation pot 311 and the first push plate 331, the first push plate 331 and the second push plate 332 are respectively connected to the two ends of the connecting rod 333, so that the first push plate 331 and the second push plate 332 can move synchronously and have the function of restoring the original state. When the follower 220 moves forward and presses the first push plate 331 forward, the second push plate 332 can be driven to move forward and the elastic member 334 is compressed. When the follower 220 moves backward, the elastic member 334 has a tendency to restore the original state. At this time, the elastic member 334 applies a backward thrust to the first push plate 331, so that the elastic member 334 pushes the first push plate 331 to move backward, and drives the second push plate 332 to move backward to the position of the original state.

[0079] Among them, the follower 220 abuts against the first push plate 331, and the second push plate 332 is drivingly connected to the cover plate 343. The second push plate 332 can adsorb the rear end of the push-pull assembly 342, and drive the front and rear movement of the first push plate 331 through the follower 220, so as to drive the front and rear movement of the second push plate 332, so that the push-pull assembly 342 can also move back and forth accordingly, making the cover plate 343 movable relative to the carrier 345. The first push plate 331 and the follower 220 are detachably connected. When the support assembly 310 is pulled out from the installation cavity, the drive assembly will not move forward with the connection assembly 330, which can avoid damage to the wires on the drive assembly caused by the wires being pulled out.

[0080] Reference Figure 3 、 Figure 7 、 Figure 8 、 Figure 9 and Figure 10 , the drive mechanism 200 is drivingly connected to the incubation assembly 340, and the follower 220 is driven by the drive member 210 to move, so as to drive the cover plate 343 to move relative to the carrier 345.

[0081] The drive mechanism 200 may include a drive member 210 and a follower 220. The drive member 210 may be a lead screw motor, and the follower 220 may be a transmission rod or a transmission plate. The output end of the drive member 210 is hinged to the lower end of the follower 220, and the output end of the drive member 210 can move back and forth. The housing mechanism 100 may further include a mounting plate 170. The middle part of the follower 220 is hinged to the mounting plate 170, so that the upper and lower ends of the follower 220 can swing around the position hinged to the mounting plate 170. When the output end of the drive member 210 moves forward, the upper end of the follower 220 swings backward. When the output end of the drive member 210 moves backward, the upper end of the follower 220 swings forward. In this way, through the forward and backward swing of the upper end of the follower 220, the first push plate 331 moves forward and backward, thereby driving the cover plate 343 to move relative to the carrier 345.

[0082] Among them, the drive member 210 is fixedly connected to the base plate 160 and is located below the incubation mechanism 300, and the drive member 210 is within the range of the top view projection of the incubation mechanism 300. The connection assembly 330, the follower 220 and the drive member 210 are arranged in a U shape, which can reduce the front and rear distance of the housing mechanism 100 and its floor area, and avoid the connection assembly 330, the follower 220 and the drive member 210 being arranged in a straight line, resulting in a large front and rear distance of the housing mechanism 100 and occupying space. It should be noted that the drive member 210 is within the range of the top view projection of the incubation mechanism 300, but the present disclosure is not limited thereto, and the drive member 210 may also be arranged at the rear side of the incubation mechanism 300.

[0083] Reference Figure 7 、Figure 8 and Figure 11 The driving mechanism 200 is drivingly connected to the incubation assembly 340. By driving the driven member 220 to move through the driving member 210, the cover plate 343 is driven to move relative to the slide 345.

[0084] The driving mechanism 200 may include a driving member 210 and a driven member 220. The driving member 210 may be a stepping motor, and the driven member 220 may be an eccentric wheel. The housing mechanism 100 may further include a mounting plate 170. The driving member 210 is fixedly connected to the mounting plate 170 in a vertical manner. The output end of the driving member 210 faces upward and is connected to the bottom end of the eccentric wheel. When the output end of the driving member 210 rotates, the eccentric wheel also rotates. The periphery of the eccentric wheel abuts against the first push plate 331. The rotation of the eccentric wheel drives the first push plate 331 to move back and forth, thereby causing the cover plate 343 to move relative to the slide 345. Among them, the driving member 210 and the driven member 220 are located below the connection assembly 330 and are not completely within the range of the top view projection of the connection assembly 330. The driving member 210 uses a stepping motor, which can save more costs compared with a lead screw motor.

[0085] Although in the above embodiments, the driving member 210 and the driven member 220 are located below the connection assembly 330, the present disclosure is not limited thereto. The driving member 210 and the driven member 220 may also be located behind the connection assembly 330. For example, the driving member 210 may be a stepping motor, and the driven member 220 may be a driven rod. The stepping motor is fixedly connected to the top of the mounting plate 170. The output end of the stepping motor is connected to the driven rod. The driven rod abuts against the first push plate 331. At this time, the driven rod and the output shaft of the stepping motor are in a straight line and perpendicular to the first push plate 331. By the forward and backward movement of the output shaft of the stepping motor, the first push plate 331 can move back and forth.

[0086] Refer to Figure 1 and Figure 12 An incubation device includes an automatic reagent dropping device 2 and the above-mentioned dynamic incubation chamber 1. The automatic reagent dropping device 2 may include a console, a robotic arm, a sampling needle, and a reagent kit. The dynamic incubation chamber 1 can be placed on the working platform of the console. The robotic arm is controlled by a controller in the console to move the sampling needle, suck the reagent from the reagent kit, then move the sampling needle to directly above the corresponding dropping hole 151, and then move down to a certain height to drop the reagent in the sampling needle tube, so that the reagent passes through the dropping hole 151 and flows between the slide 345 and the cover plate 343. Through the cooperation of the automatic reagent dropping device 2 and the dynamic incubation chamber 1, the automatic dropping and staining operation of the specimen on the slide 345 can be realized. It should be noted that the automatic reagent dropping device 2 and its working principle belong to the prior art and will not be elaborated here.

[0087] In the embodiments of the present disclosure, the terms "first", "second", and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance; the term "plurality" means two or more, unless otherwise clearly defined. Terms such as "installed", "connected", "coupled", "fixed", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; "coupled" can be a direct connection or an indirect connection through an intermediate medium. For those of ordinary skill in the art, the specific meanings of the above terms in the embodiments of the present disclosure can be understood according to specific circumstances.

[0088] In the description of the embodiments of the present disclosure, it should be understood that the orientation or positional relationship indicated by the terms "upper", "lower", "left", "right", "front", "rear", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the embodiments of the present disclosure and simplifying the description, rather than indicating or implying that the device or unit referred to must have a specific direction, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on the embodiments of the present disclosure.

[0089] Of course, once the above description of the representative embodiments is carefully considered, those skilled in the art will readily understand that various modifications, additions, substitutions, deletions, and other changes can be made to these specific embodiments, and such changes are within the scope of the principles of the present invention. Therefore, the foregoing detailed description should be clearly understood to be given by way of illustration and example only, and the spirit and scope of the present invention are defined only by the appended claims and their equivalents.

Claims

1. A dynamic incubator, characterized in that, Comprising: A housing mechanism, within which there is an installation cavity; A driving mechanism, the driving mechanism includes a driving member and a driven member, and the driving member is drivingly connected to the driven member; An incubation mechanism, the incubation mechanism includes a support assembly and an incubation assembly, the support assembly is connected to the inner wall of the installation cavity; the support assembly includes an incubation pot provided with an incubation groove, and the incubation assembly is detachably arranged in the incubation groove; the driven member is drivingly connected to the incubation assembly, and the incubation assembly includes a cover plate and a slide; The driving member can drive the driven member to move, so as to drive the cover plate to move relative to the slide.

2. The dynamic incubator according to claim 1, characterized in that, The driving member is connected to the housing mechanism, and is located below the incubation mechanism, and the driving member is within the range of the top view projection of the incubation mechanism; the driven member is hinged to the housing mechanism, and the lower end of the driven member is hinged to the output end of the driving member, and the driving member can drive the upper end of the driven member to swing back and forth.

3. The dynamic incubator according to claim 1, characterized in that, The support assembly is movably connected to the inner wall of the installation cavity in a push-pull manner.

4. The dynamic incubator according to claim 3, characterized in that, The support assembly further includes a support frame and a pair of slide rails, and the support frame is slidably connected to the side wall of the installation cavity through the pair of slide rails; the incubation pot is connected to the support frame, and the incubation pot can drive the incubation assembly to move back and forth.

5. The dynamic incubator according to claim 1, characterized in that, The support assembly further includes a connection assembly, the connection assembly includes a first push plate, a second push plate, a connecting rod and an elastic member, the first push plate and the second push plate are respectively connected to both ends of the connecting rod, the connecting rod passes through the incubation pot, and the elastic member is sleeved on the connecting rod and is located between the incubation pot and the first push plate; the driven member can abut against the first push plate, and the second push plate is drivingly connected to the cover plate.

6. The dynamic incubator according to claim 1, characterized in that, The support assembly includes at least two of the incubation pots, and different incubation pots are arranged side by side.

7. The dynamic incubator according to claim 1, characterized in that, Limit posts are arranged at the bottom of the incubation groove, and limit grooves are arranged at the bottom of the incubation assembly. The limit posts can cooperate with the limit grooves to limit the shaking of the incubation assembly.

8. The dynamic incubator according to claim 4, characterized in that, The support assembly further includes a heating member and a heat insulation member. The heating member is connected to the bottom of the incubation pot, and the heat insulation member is arranged below the heating member and is connected to the support frame.

9. The dynamic incubator according to claim 1, characterized in that, The housing mechanism includes an upper cover that is arranged above the incubation pot and can be opened and closed. A plurality of liquid dropping holes are opened on the upper cover. The plurality of liquid dropping holes correspond to the plurality of slides one by one, and the reagent can pass through the liquid dropping holes and flow between the slide and the cover plate.

10. An incubation device, comprising an automatic reagent dropping device, characterized in that, The incubation device further includes the dynamic incubation box according to any one of claims 1 to 9, and the automatic reagent dropping device can drop the reagent between the cover plate and the slide.

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