Edible and medicinal fungus fiber-based induction chip embedded paper as well as preparation method and application of edible and medicinal fungus fiber-based induction chip embedded paper

Abstract

The invention aims to provide edible and medicinal fungus fiber-based induction chip embedded paper and a preparation method and application thereof, and relates to the technical field of microbial fermentation, the preparation method of the edible and medicinal fungus fiber-based induction chip embedded paper comprises the following steps: culturing edible and medicinal fungus aerial hyphae; the method comprises the following steps: drying edible and medicinal fungus aerial hyphae with a sensing chip, preparing edible and medicinal fungus fungus fibers, preparing edible and medicinal fungus fungus fiber paper pulp, and preparing edible and medicinal fungus fungus fiber paper embedded with the sensing chip. The prepared fungus fiber-based sensing chip embedded paper has good strength and certain waterproof performance, and can be used as packaging paper to be applied to multiple fields of intelligent packaging, logistics tracking, medical monitoring and the like.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial fermentation, and particularly relates to an edible and medicinal fungal mycelial fiber-based induction chip embedded paper, a preparation method thereof, and an application thereof. Background Art

[0002] As a widely used material, paper plays an irreplaceable role in society. Traditional paper is mainly made from wood fibers, through strong alkali and strong acid treatment, and then through processes such as bleaching, papermaking, drying, and post-treatment; it has problems such as large consumption of wood resources and environmental pollution in the production process. Mycelial fiber, as a new type of renewable resource, has the advantages of scalable cultivation, fast growth rate, easy degradation after use, and environmental friendliness, and has received wide attention globally.

[0003] Mycelial fiber mainly comes from edible and medicinal fungi and is a renewable resource. Compared with wood, the cultivation cycle of edible and medicinal fungi is short, and the cultivation cycle of the mycelium of edible and medicinal fungi is even shorter. Preparing paper with edible and medicinal fungal mycelial fiber as the main raw material helps to reduce the dependence of the paper industry on forests and does not require alkali or acid treatment. Mycelial fiber paper has good physical properties such as flexibility, strength, and stiffness. At the same time, by treating the mycelial fiber and optimizing the paper preparation process, the surface smoothness, gloss, and other properties of the paper can be adjusted, expanding the application fields of the paper.

[0004] At present, the anti-counterfeiting and traceability of regular products have become hot issues of concern to production enterprises and consumers. Given the wide application basis of paper in fields such as packaging, printing, and labeling, the present invention combines mycelial fiber paper with an induction chip, which can provide a new solution for enterprise product management and anti-counterfeiting. At present, most existing products use industrial glue to bond the induction chip to the paper, which not only affects the performance of the chip but also causes environmental pollution. The edible and medicinal fungal mycelial fiber-based induction chip embedded paper of the present invention is a new type of intelligent and environmentally friendly paper, with the advantages of being renewable, degradable, and pollution-free; at the same time, the induction chip can realize functions such as anti-counterfeiting inspection, traceability, and counting. It is embedded inside the mycelial fiber paper, increasing the concealment of the chip and endowing the paper with more functions and application values; in addition, based on the unique physical properties of mycelial fiber paper, even after multiple folds and rubs, it will not affect the induction function of the embedded chip, ensuring the stable function of the chip. Therefore, the present invention can provide an effective method for enterprises in aspects such as product anti-counterfeiting inspection, traceability, and counting, reducing the technical, labor, and time costs of enterprises, and not only having broad market application prospects but also having good ecological benefits. Summary of the Invention

[0005] The present invention provides an edible and medicinal fungus mycelial fiber-based induction chip embedded paper, its preparation method and application, pioneering a green paper preparation process that does not use strong alkalis and strong acids and is environmentally friendly; the prepared edible and medicinal fungus mycelial fiber-based induction chip embedded paper is a new type of environmentally friendly paper with the advantages of being renewable and degradable; the induction chip is naturally embedded during the forming process of the edible and medicinal fungus mycelial fiber-based induction chip embedded paper without affecting the chip function. At the same time, the edible and medicinal fungus mycelial fiber-based induction chip embedded paper has good flexibility and elasticity, and can play a role in buffering and protecting the induction chip under external forces such as folding and kneading, ensuring the functional stability of the induction chip, and can realize functions such as forgery detection, traceability, and counting.

[0006] According to one aspect of the present invention, there is provided a method for preparing an edible and medicinal fungus mycelial fiber-based induction chip embedded paper, the method comprising the following steps: Step 1, culturing the aerial mycelia of edible and medicinal fungi: Inoculate the strains of edible and medicinal fungi into a solid medium with an inoculation amount of 5% - 10%, and place them in a culture room for culturing to obtain the aerial mycelia of edible and medicinal fungi; Step 2, drying the aerial mycelia of edible and medicinal fungi with an induction chip: Place a surface-clean induction chip in the aerial mycelia of edible and medicinal fungi, culture for 1 - 3 days to obtain the aerial mycelia of edible and medicinal fungi with an induction chip, take it out and perform drying treatment to obtain the dried aerial mycelia of edible and medicinal fungi with an induction chip; Step 3, preparing the mycelial fibers of edible and medicinal fungi: Take out the induction chip covered with the aerial mycelia of edible and medicinal fungi from the dried aerial mycelia of edible and medicinal fungi with an induction chip for standby, and then grind the remaining dried aerial mycelia of edible and medicinal fungi with water to make a mycelial fiber liquid of edible and medicinal fungi. After centrifuging multiple times, obtain the mycelial fibers of edible and medicinal fungi; Step 4, preparing the mycelial fiber pulp of edible and medicinal fungi: Add water to the mycelial fibers of edible and medicinal fungi to prepare a mycelial fiber suspension of edible and medicinal fungi, then add sodium carboxymethylcellulose to obtain suspension 1, stir with a magnetic stirrer, then add cationic starch to obtain suspension 2, and then stir with a magnetic stirrer to obtain the mycelial fiber pulp of edible and medicinal fungi; Step 5, preparing the edible and medicinal fungus mycelial fiber-based induction chip embedded paper: The edible and medicinal fungus mycelial fiber pulp is added with water to prepare an edible and medicinal fungus pulp suspension, which is filtered by a vacuum filter to obtain two edible and medicinal fungus mycelial fiber cakes. One of the edible and medicinal fungus mycelial fiber cakes is laid flat in a mold. After placing the induction chip covered with the edible and medicinal fungus aerial mycelium in step 3, another edible and medicinal fungus mycelial fiber cake is placed to form an edible and medicinal fungus mycelial fiber cake embedded with the induction chip. It is placed in a paper former to be formed, dehydrated and dried to obtain an edible and medicinal fungus mycelial fiber-based induction chip-embedded paper.

[0007] Preferably, the edible and medicinal fungus in step 1 is selected from any one of Ganoderma lucidum, Phellinus linteus, Lentinula edodes, Auricularia auricula, Hericium erinaceus, Grifola frondosa, Poria cocos.

[0008] Preferably, the preparation method of the solid medium in step 1 is: loading the solid medium raw material into a cultivation bag, making small holes in the center of the bag body of the cultivation bag; sterilizing at 115-121 °C for 20-120 min; waiting for the solid medium raw material to cool to 25-30 °C to obtain the solid medium.

[0009] Preferably, the solid medium in step 1 includes a solid medium raw material and water, and the solid medium raw material includes: 70%-78% of sawdust, 20%-26% of wheat bran, 1%-2% of gypsum, and 1%-2% of sugar.

[0010] Preferably, after step 1, the method further includes: screening the edible and medicinal fungus aerial mycelium with a white color, no abnormal discoloration, and uniform and dense growth by the naked eye observation method; screening the edible and medicinal fungus aerial mycelium with thick morphology and a hyphal diameter of not less than 1 μm by the scanning electron microscope observation method.

[0011] Preferably, the specific method of the drying treatment in step 2 is: putting the edible and medicinal fungus aerial mycelium with the induction chip into a constant temperature forced air drying oven at a temperature of 45-65 °C for 5-10 h for drying treatment.

[0012] Preferably, after step 2, the method further includes: screening the dried edible and medicinal fungus aerial mycelium with the induction chip by the vacuum drying method to ensure that the moisture content does not exceed 4%.

[0013] Preferably, the specific method for preparing the edible and medicinal fungal mycelial fiber in step 3 is as follows: Take out the induction chip covered with the aerial mycelium of the edible and medicinal fungus from the dried aerial mycelium of the edible and medicinal fungus with an induction chip and set it aside. Then, grind the remaining dried aerial mycelium of the edible and medicinal fungus with water to make an edible and medicinal fungal mycelial fiber solution. Place the edible and medicinal fungal mycelial fiber solution in a water bath at 45-65°C and stir it at a speed of 200-300 r / min for 60-120 min. Then, place the edible and medicinal fungal mycelial fiber solution in a centrifuge and centrifuge it at 3500-4500 r / min for 10-15 min. Rinse the centrifuged precipitate with pure water and then centrifuge it at 3500-4500 r / min for 10-15 min. The obtained precipitate is the edible and medicinal fungal mycelial fiber.

[0014] Preferably, the specific method for grinding the remaining dried aerial mycelium of the edible and medicinal fungus with water in step 3 is as follows: Put the dried aerial mycelium of the edible and medicinal fungus into a pulper, add pure water to obtain an aerial mycelium suspension of the edible and medicinal fungus, and make the concentration of the aerial mycelium of the edible and medicinal fungus in the aerial mycelium suspension of the edible and medicinal fungus be 40-60 g / 100 mL. Set the gap distance between the grinding discs to 1-5 mm, the power of the flying knife rotor to 1.1 kW, and the rotation speed of the flying knife to 1430-1470 r / min. Grind the aerial mycelium suspension of the edible and medicinal fungus for 5-15 min to obtain an edible and medicinal fungal mycelial fiber solution.

[0015] Preferably, after step 3, the method further includes: Screening the edible and medicinal fungal mycelial fiber with a laser confocal fluorescence microscope to have a length of not less than 20 μm and a width of not more than 1 μm.

[0016] Preferably, the specific method for preparing the edible and medicinal fungal mycelial fiber pulp in step 4 is as follows: Prepare an edible and medicinal fungal mycelial fiber suspension by adding water to the edible and medicinal fungal mycelial fiber, add sodium carboxymethylcellulose to obtain suspension 1, stir it with a magnetic stirrer, and stir suspension 1 at 45-65°C at a speed of 150-200 r / min for 15-45 min. Then add cationic starch to obtain suspension 2, and then stir it with a magnetic stirrer. Stir suspension 2 at 45-65°C at a speed of 150-200 r / min for 15-45 min to obtain the edible and medicinal fungal mycelial fiber pulp.

[0017] Preferably, the concentration of the edible and medicinal fungal mycelial fiber in the edible and medicinal fungal mycelial fiber suspension in step 4 is 30-60 g / 100 mL, the concentration of sodium carboxymethylcellulose in suspension 1 is 0.2-1.2 g / 100 mL, and the concentration of cationic starch in suspension 2 is 0.4-1.4 g / 100 mL.

[0018] Preferably, after step 4, the method further comprises: using a potentiometer to screen the edible and medicinal fungus fiber pulp to find that the surface charge is not less than 8 mV, and using a particle size analyzer to screen the edible and medicinal fungus fiber pulp to find that the average particle size is not less than 1 mm.

[0019] Preferably, the concentration of edible and medicinal fungus fiber in the edible and medicinal fungus pulp suspension in step 5 is 0.7-1.4 g / 100 mL; the gram weight of the edible and medicinal fungus fiber filter cake embedded with the sensing chip in step 5 is 50-100 g / m2; the specific conditions for dehydration and drying in step 5 are: heating power 10-20 kw, drying temperature 40-60°C, and drying time 12-24 h.

[0020] Preferably, after step 5, the method further comprises: using a contact angle meter to screen that the contact angle of the paper with embedded edible and medicinal fungi fiber-based sensor chip and water is not less than 79°; using a folding endurance meter to screen that the paper with embedded edible and medicinal fungi fiber-based sensor chip will not affect the sensing function of the sensor chip after 100 foldings; using an electronic universal tester to screen that the tensile strength of the paper with embedded edible and medicinal fungi fiber-based sensor chip is not less than 180MPa; using a smoothness meter to screen that the surface smoothness of the paper with embedded edible and medicinal fungi fiber-based sensor chip is not less than 600s; using a stiffness meter to screen that the stiffness of the paper with embedded edible and medicinal fungi fiber-based sensor chip is not less than 0.1mNm; using a glossiness meter to screen that the glossiness of the paper with embedded edible and medicinal fungi fiber-based sensor chip is not less than 70%.

[0021] According to another aspect of the present invention, there is also provided paper prepared by the method for preparing paper embedded with an edible and medicinal fungus fiber-based sensor chip.

[0022] According to another aspect of the present invention, there is also provided the use of paper embedded with a sensor chip based on edible and medicinal fungi fibers as packaging paper.

[0023] The beneficial effects of the present invention are as follows: the method for preparing paper using edible and medicinal fungus fiber as raw material helps to reduce dependence on traditional wood fiber, and meets the requirements of environmental protection and sustainable development; the paper with embedded sensor chip based on edible and medicinal fungus fiber is the first to have good strength and certain waterproof performance, thus ensuring the stability and reliability of the paper with embedded sensor chip based on edible and medicinal fungus fiber. The paper with embedded sensor chip based on edible and medicinal fungus fiber prepared by the present invention can be applied to multiple fields such as intelligent packaging, logistics tracking, and medical monitoring, and has broad market prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a specific implementation flow chart of the present invention.

[0025] Figure 2 It is the apparent morphological diagram of Ganoderma lucidum aerial hyphae.

[0026] Figure 3 It is the morphological diagram of Ganoderma lucidum aerial hyphae observed by scanning electron microscope.

[0027] Figure 4 It is the schematic diagram of the induction chip.

[0028] Figure 5 It is the schematic diagram of the surface of the induction chip surrounded and intertwined by Ganoderma lucidum aerial hyphae.

[0029] Figure 6 It is the morphological diagram of Ganoderma lucidum fungal fiber observed by laser confocal fluorescence microscope.

[0030] Figure 7 It is the morphological diagram of the Ganoderma lucidum fungal fiber-based induction chip embedded paper placed in the mold before molding.

[0031] Figure 8 It is the morphological diagram of the Ganoderma lucidum fungal fiber-based induction chip embedded paper before molding.

[0032] Figure 9 It is the morphological diagram of the Ganoderma lucidum fungal fiber-based induction chip embedded paper after molding. Detailed implementation methods

[0033] Definition: The term "edible and medicinal mushroom fungal fiber" used in this article refers to: a type of dietary fiber present in the cell walls of edible and medicinal mushrooms, mainly composed of polysaccharide substances, including β-glucan, chitin, cellulose, etc.

[0034] The term "aerial hyphae" used in this article refers to: a type of hyphae of edible and medicinal mushrooms, which is the hyphae that extends into the space after the growth and development of the hyphae in the culture medium. On solid culture media, aerial hyphae are visible to the naked eye and often cover the surface of the culture medium, presenting a fluffy appearance. Aerial hyphae have multiple important physiological functions. On the one hand, it can further extend and expand to explore new nutrient sources and search for suitable growth environments; on the other hand, when aerial hyphae develop to a certain stage, they will differentiate into reproductive organs, such as producing sporangia, conidiophores, etc., for producing various asexual or sexual spores to achieve the reproduction and spread of edible and medicinal mushrooms. During the artificial cultivation of edible and medicinal mushrooms, the growth status of aerial hyphae is one of the important indicators for judging whether the cultivation conditions are suitable. For example, if the aerial hyphae grow too vigorously, it may affect the formation and development of fruiting bodies, which may be related to factors such as humidity, temperature, and ventilation in the cultivation environment. On the contrary, if the aerial hyphae grow poorly, it may mean insufficient nutrients or unsuitable environmental conditions, and the cultivation conditions need to be adjusted.

[0035] The term "solid culture matrix" as used herein refers to a solid substance that provides nutrition and a habitat for the growth, development and reproduction of edible and medicinal fungi, and its ingredients include: (1) Main nutrient sources, including carbon sources (such as glucose, sucrose, starch, sawdust, corn cobs, etc., which provide energy for the growth of edible and medicinal fungi and synthesize the carbon skeleton of cell substances) and nitrogen sources (such as peptone, yeast powder, soybean cake powder, bran, corn flour, etc., which meet the fungi's demand for nitrogen and are used to synthesize nitrogen-containing biological molecules such as proteins and nucleic acids).

[0036] (2) Inorganic salts: provide the mineral elements necessary for the growth of edible and medicinal fungi, such as phosphorus, potassium, magnesium, calcium, etc., which are generally met by adding inorganic salts such as potassium dihydrogen phosphate, magnesium sulfate, and calcium sulfate. These elements play an important role in cell metabolism and enzyme activity regulation.

[0037] (3) Growth factors, including vitamins, amino acids, nucleic acids and other organic substances that are essential for the growth of edible and medicinal fungi but cannot be synthesized by themselves or are synthesized in insufficient quantities. For example, yeast extract contains rich growth factors such as B vitamins, which can promote the growth and development of fungi.

[0038] (4) Coagulant, the most commonly used is agar, which can keep the culture medium solid at room temperature and provide a support structure for the growth of fungal hyphae.

[0039] Embodiment 1 of the present invention: Step 1, cultivation of aerial mycelium of Ganoderma lucidum: 936 g of sawdust, 240 g of bran, 12 g of gypsum and 12 g of sugar are used as raw materials of solid culture matrix, stirred evenly, and 1500 g of purified water is added to obtain a solid culture matrix, and the water content of the solid culture matrix is ​​55%; the solid culture matrix is ​​put into a cultivation bag, which can be a cultivation plastic bag, and a small hole is punched in the center of the bag body of the cultivation bag; then the cultivation bag is sterilized at 121° C. for 60 minutes, and the bag is cooled to 25° C. to obtain a solid culture medium; in an ultra-clean workbench, a Ganoderma lucidum seed is inoculated into the solid culture medium with an inoculation amount of 5%; the solid culture medium is placed in a culture room at a temperature of 28° C. and a humidity of 70%, and cultured for 10 days to form white, fluffy, fuzzy, and radially edged Ganoderma lucidum aerial mycelium; the Ganoderma lucidum aerial mycelium with white color, no abnormal discoloration, uniform growth and dense growth is selected by naked eye observation, such as Figure 2 As shown; scanning electron microscopy was used to screen Ganoderma lucidum aerial hyphae with a robust morphology and a hyphae diameter of not less than 1 μm, such as Figure 3 shown.

[0040] Step 2, Drying of Ganoderma lucidum aerial mycelium with sensor chip: Place a circular induction chip with a clean surface, a diameter of 1 cm, and a thickness of 0.3 mm in the aerial hyphae of Ganoderma lucidum growing on a solid medium obtained in Step 1. The induction chip is as shown in Figure 4 . After 2 days of cultivation, the surface of the induction chip is surrounded and intertwined by the aerial hyphae of Ganoderma lucidum, as shown in Figure 5 . The aerial hyphae of Ganoderma lucidum with the induction chip are obtained. Peel the aerial hyphae of Ganoderma lucidum with the induction chip from the solid medium and place them in a thermostatic air-blast drying oven. Dry them at a temperature of 45 °C for 6 h to obtain dried aerial hyphae of Ganoderma lucidum with the induction chip. Use the vacuum drying method to screen the moisture content of the dried aerial hyphae of Ganoderma lucidum with the induction chip not exceeding 4%.

[0041] Step 3: Preparation of Ganoderma lucidum fungal fibers: Step 3: The dried aerial hyphae of Ganoderma lucidum with the induction chip obtained in Step 2 can be regarded as two parts: one part is the induction chip covered with the aerial hyphae of Ganoderma lucidum on the surface, and the other part is the dried aerial hyphae of Ganoderma lucidum. First, take out the induction chip covered with the aerial hyphae of Ganoderma lucidum for standby. Then, put 120 g of dried aerial hyphae of Ganoderma lucidum into a pulper and add pure water to obtain an aerial hyphae suspension of Ganoderma lucidum, so that the concentration of the aerial hyphae of Ganoderma lucidum in the aerial hyphae suspension is 60 g / 100 mL. Set the gap distance between the grinding discs to 1 mm, the power of the flying knife rotor to 1.1 kW, and the rotational speed of the flying knife to 1470 r / min. Grind the aerial hyphae of Ganoderma lucidum for 5 min to obtain a Ganoderma lucidum fungal fiber liquid. Place the Ganoderma lucidum fungal fiber liquid in a water bath at 65 °C and stir it at a rotational speed of 200 r / min for 120 min. Then, place the Ganoderma lucidum fungal fiber liquid in a centrifuge and centrifuge it at 4500 r / min for 15 min. Rinse the centrifuged precipitate with pure water and then centrifuge it at 4500 r / min for 15 min. The obtained precipitate is Ganoderma lucidum fungal fibers. Use a laser confocal fluorescence microscope to screen that the length of the Ganoderma lucidum fungal fibers is not less than 20 μm and the width is not more than 1 μm, as shown in Figure 6 .

[0042] Step 4: Preparation of Ganoderma lucidum fungal fiber pulp: For the 108 g of Ganoderma lucidum mycelial fiber obtained in Step 3, prepare a Ganoderma lucidum mycelial fiber suspension with a concentration of 30 g / 100 mL using 360 mL of purified water; add sodium carboxymethylcellulose to the Ganoderma lucidum mycelial fiber suspension to obtain Suspension 1, such that the concentration of sodium carboxymethylcellulose in Suspension 1 is 0.2 g / 100 mL, and use a magnetic stirrer to stir Suspension 1 at 65 °C at a rotation speed of 150 r / min for 30 min; then, add cationic starch to Suspension 1 to obtain Suspension 2, such that the concentration of cationic starch in Suspension 2 is 0.5 g / 100 mL, and use a magnetic stirrer to stir Suspension 2 at 65 °C at a rotation speed of 150 r / min for 45 min to obtain Ganoderma lucidum mycelial fiber pulp; use a potentiometer to screen the surface charge of the Ganoderma lucidum mycelial fiber pulp to be not less than 8 mV; use a particle size analyzer to screen the average particle size of the Ganoderma lucidum mycelial fiber pulp to be not less than 1 mm.

[0043] Step 5, Preparation of Ganoderma lucidum mycelial fiber-based inductive chip-embedded paper: For 360 mL of the Ganoderma lucidum mycelial fiber pulp obtained in Step 4, prepare a Ganoderma lucidum mycelial pulp suspension using 7200 mL of purified water, with the concentration of Ganoderma lucidum mycelial fiber in the Ganoderma lucidum mycelial pulp suspension being 1.5 g / 100 mL. Divide the Ganoderma lucidum mycelial pulp suspension into two portions with a volume ratio of 1:1, and use a vacuum filter to filter the two portions of the Ganoderma lucidum mycelial pulp suspension twice to obtain two Ganoderma lucidum mycelial fiber cakes; place one of the Ganoderma lucidum mycelial fiber cakes flat in a square mold with a side length of 20 cm, and use a squeegee to level the surface of the Ganoderma lucidum mycelial fiber cake; then, place the inductive chip with Ganoderma lucidum aerial mycelium on the surface, which was mentioned in Step 3, in the center of the Ganoderma lucidum mycelial fiber cake, and then place the other Ganoderma lucidum mycelial fiber cake on top of the first leveled Ganoderma lucidum mycelial fiber cake, and use a squeegee to level the surface of the Ganoderma lucidum mycelial fiber cake; the total grammage of the two overlapping Ganoderma lucidum mycelial fiber cakes is 70 g / m 2 ; Remove the mold, and place the overlapping Ganoderma lucidum mycelial fiber cakes in a sheet former. As Figure 7 shown, set the heating power to 10 kw, the drying temperature to 45 °C, and the drying time to 12 h to obtain Ganoderma lucidum mycelial fiber-based inductive chip-embedded paper. As Figure 9As shown; a contact angle measuring instrument is used to screen that the contact angle between the Ganoderma lucidum fungal fiber-based induction chip embedded paper and water is not less than 79°; a folding endurance tester is used to screen that the Ganoderma lucidum fungal fiber-based induction chip embedded paper will not affect the induction function of the induction chip after 100 folds; an electronic universal tester is used to screen that the tensile strength of the Ganoderma lucidum fungal fiber-based induction chip embedded paper is not less than 180 MPa; a smoothness tester is used to screen that the surface smoothness of the Ganoderma lucidum fungal fiber-based induction chip embedded paper is not less than 600 s; a stiffness tester is used to screen that the stiffness of the Ganoderma lucidum fungal fiber-based induction chip embedded paper is not less than 0.1 mNm; a glossmeter is used to screen that the gloss of the Ganoderma lucidum fungal fiber-based induction chip embedded paper is not less than 70%.

[0044] Preparation mechanism of edible and medicinal mushroom mycelium fiber-based sensing chip embedded paper: Edible and medicinal mushroom mycelium fibers contain a large amount of β-glucan. β-glucan is a polysaccharide composed of glucose monomers connected by β-1,4 glycosidic bonds, and its molecular structure contains a large number of hydroxyl (-OH) groups. Sodium carboxymethyl cellulose is a water-soluble polymer compound, and its molecular chain has carboxyl (-COOH) and hydroxyl (-OH). In the pulp mainly composed of edible and medicinal mushroom mycelium fibers, hydrogen bond interactions, electrostatic interactions, etc. can form between the hydroxyl groups on the β-glucan molecules of edible and medicinal mushroom mycelium fibers and the carboxyl or hydroxyl groups on the sodium carboxymethyl cellulose molecules, and hydrophobic interactions also occur in the hydrophobic regions of the two molecules; that is, the long-chain structure of sodium carboxymethyl cellulose can play a "bridge" role between edible and medicinal mushroom mycelium fibers, further enhancing the interaction between edible and medicinal mushroom mycelium fibers, thereby improving the physical strength properties such as the tensile strength and folding endurance of the paper, and can improve the uniformity of the edible and medicinal mushroom mycelium fiber-based sensing chip embedded paper. In addition, in the edible and medicinal mushroom mycelium fiber pulp, the surface of the edible and medicinal mushroom mycelium fibers usually carries negative charges, while cationic starch carries positive charges. Based on the principle of electrostatic attraction, cationic starch can adsorb on the surface of edible and medicinal mushroom mycelium fibers, fill the gaps between edible and medicinal mushroom mycelium fibers, increase the contact area between edible and medicinal mushroom mycelium fibers, and promote the combination between edible and medicinal mushroom mycelium fibers; at the same time, the hydroxyl groups on the cationic starch molecular chain can also form hydrogen bonds with the hydroxyl groups on the surface of edible and medicinal mushroom mycelium fibers, enhancing the binding force between edible and medicinal mushroom mycelium fibers and further improving the strength of the edible and medicinal mushroom mycelium fiber-based paper. During the pulping process, cationic starch adsorbs on the surface of edible and medicinal mushroom mycelium fibers, changes the charge distribution on the surface of edible and medicinal mushroom mycelium fibers, reduces the electrostatic repulsion between edible and medicinal mushroom mycelium fibers, enables edible and medicinal mushroom mycelium fibers to be more evenly dispersed in the pulp; and to a certain extent, it can hinder the rapid sedimentation of edible and medicinal mushroom mycelium fibers, extend the suspension time of edible and medicinal mushroom mycelium fibers in the pulp, is conducive to the uniform distribution of edible and medicinal mushroom mycelium fibers, and thus improves the uniformity of the paper.

[0045] After the above specific implementation steps, the Ganoderma lucidum mycelium fiber-based sensing chip embedded paper is processed, and its apparent morphology is as Figure 6 shown.

[0046] According to another aspect of the present invention, there is also provided a paper prepared by the method of the Ganoderma lucidum mycelium fiber-based sensing chip embedded paper.

[0047] According to another aspect of the present invention, there is also provided the application of the Ganoderma lucidum mycelium fiber-based sensing chip embedded paper as packaging paper.

[0048] Example 2 of the present invention: The main difference from Example 1 lies in that in Step 1, the composition of the solid culture medium substrate is different: specifically, it is 888 g of wood chips, 264 g of wheat bran, 12 g of gypsum, and 12 g of sugar, that is, 74% of wood chips, 22% of wheat bran, 1% of gypsum, and 1% of sugar. The other operation steps are the same as those in Example 1.

[0049] Example 3 of the present invention: The main difference from Example 1 lies in that in Step 1, the composition of the solid culture medium substrate is different: specifically, it is 864 g of wood chips, 264 g of wheat bran, 24 g of gypsum, and 24 g of sugar, that is, 72% of wood chips, 26% of wheat bran, 1% of gypsum, and 1% of sugar. The other operation steps are the same as those in Example 1.

[0050] Example 4 of the present invention: The main difference from Example 1 lies in that in Step 1, the inoculation amount of Ganoderma lucidum strains is 7%, and the other operation steps are the same as those in Example 1.

[0051] Example 5 of the present invention: The main difference from Example 1 lies in that in Step 1, the inoculation amount of Ganoderma lucidum strains is 9%, and the other operation steps are the same as those in Example 1.

[0052] Effect test: Determination of parameters of Ganoderma lucidum mycelium fiber-based induction chip embedded paper: Measure various parameters of the Ganoderma lucidum mycelium fiber-based induction chip embedded paper prepared through Examples 1 - 5. Use a contact angle measuring instrument to measure the contact angle between the Ganoderma lucidum mycelium fiber-based induction chip embedded paper and water, use a folding endurance tester to measure the influence of 100 folds of the Ganoderma lucidum mycelium fiber-based induction chip embedded paper on the induction function of the induction chip, use an electronic universal tester to measure the tensile strength of the Ganoderma lucidum mycelium fiber-based induction chip embedded paper, use a smoothness tester to measure the surface smoothness of the Ganoderma lucidum mycelium fiber-based induction chip embedded paper, use a stiffness tester to measure the stiffness of the Ganoderma lucidum mycelium fiber-based induction chip embedded paper, and use a glossiness tester to measure the glossiness of the Ganoderma lucidum mycelium fiber-based induction chip embedded paper. The specific values are shown in Table 1: Table 1. Parameters of the Ganoderma lucidum mycelium fiber-based induction chip embedded paper prepared through Examples 1 - 5.

[0053]

[0054] As can be seen from Table 1, compared with Example 1, Examples 2 and 3 are Ganoderma lucidum mycelium fiber-based induction chip-embedded papers prepared from different ratios of solid culture medium raw materials, and there are some differences in their respective parameters. Example 1 meets the following conditions: the contact angle between the Ganoderma lucidum mycelium fiber-based induction chip-embedded paper and water is not less than 79°; the Ganoderma lucidum mycelium fiber-based induction chip-embedded paper will not affect the induction function of the induction chip after 100 folds; the tensile strength of the Ganoderma lucidum mycelium fiber-based induction chip-embedded paper is not less than 180 MPa; the surface smoothness of the Ganoderma lucidum mycelium fiber-based induction chip-embedded paper is not less than 600 s; the stiffness of the Ganoderma lucidum mycelium fiber-based induction chip-embedded paper is not less than 0.1 mNm; the gloss of the Ganoderma lucidum mycelium fiber-based induction chip-embedded paper is not less than 70%. Compared with Example 1, Examples 4 and 5 are Ganoderma lucidum mycelium fiber-based induction chip-embedded papers prepared from different inoculation amounts of Ganoderma lucidum strains, and there are some differences in their respective parameters. Example 4 meets the following conditions: the contact angle between the Ganoderma lucidum mycelium fiber-based induction chip-embedded paper and water is not less than 79°; the Ganoderma lucidum mycelium fiber-based induction chip-embedded paper will not affect the induction function of the induction chip after 100 folds; the tensile strength of the Ganoderma lucidum mycelium fiber-based induction chip-embedded paper is not less than 180 MPa; the surface smoothness of the Ganoderma lucidum mycelium fiber-based induction chip-embedded paper is not less than 600 s; the stiffness of the Ganoderma lucidum mycelium fiber-based induction chip-embedded paper is not less than 0.1 mNm; the gloss of the Ganoderma lucidum mycelium fiber-based induction chip-embedded paper is not less than 70%.

[0055] The steps in the method of the embodiments of the present invention can be adjusted, combined and deleted according to actual needs. In the solution of the present invention, the descriptions of each embodiment have their own emphases. For the parts not detailed or recorded in a certain embodiment, reference can be made to the relevant descriptions of other embodiments. The technical features of the technical solution of the present invention can be combined arbitrarily. For the sake of brevity of description, not all possible combinations of the technical features in the embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as the scope recorded in the present invention.

[0056] The above are only the preferred embodiments of the present application. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present application, several improvements and retouches can be made, and these improvements and retouches should also be regarded as the protection scope of the present application.

Claims

1. A method for preparing paper embedded with a sensor chip based on edible and medicinal fungi fiber, characterized in that: The following steps are involved: Step 1, cultivation of aerial hyphae of edible and medicinal fungi: The edible and medicinal fungus strains are inoculated into a solid culture medium with an inoculation amount of 5% to 10%, and cultured in a culture room to obtain aerial hyphae of the edible and medicinal fungus; Step 2, drying of aerial hyphae of edible and medicinal fungi with a sensor chip: A sensor chip with a clean surface is placed in the aerial hyphae of edible and medicinal fungi, and the aerial hyphae with the sensor chip is cultured for 1 to 3 days to obtain the aerial hyphae of edible and medicinal fungi, which is then taken out and dried to obtain dried aerial hyphae of edible and medicinal fungi with the sensor chip; Step 3, preparation of edible and medicinal fungus fiber: The sensor chip with the edible and medicinal fungus aerial hyphae on the surface is taken out from the dried edible and medicinal fungus aerial hyphae with the sensor chip for later use, and then the remaining dried edible and medicinal fungus aerial hyphae are ground with water to prepare edible and medicinal fungus fungus fiber liquid, and after multiple centrifugation, edible and medicinal fungus fungus fiber is obtained; Step 4, preparation of edible and medicinal fungus fiber pulp: Add water to edible and medicinal fungus fiber to prepare edible and medicinal fungus fiber suspension, then add sodium carboxymethyl cellulose to obtain suspension 1, stir with a magnetic stirrer, then add cationic starch to obtain suspension 2, and stir with a magnetic stirrer to obtain edible and medicinal fungus fiber pulp; Step 5, preparation of edible and medicinal fungus fiber paper embedded with a sensing chip: Add water to edible and medicinal fungus fiber pulp to prepare an edible and medicinal fungus pulp suspension, filter with a vacuum filter to obtain two edible and medicinal fungus fiber filter cakes, spread one of the edible and medicinal fungus fiber filter cakes in a mold, put the sensing chip with edible and medicinal fungus aerial hyphae on the surface in step 3 into it, and then put another edible and medicinal fungus fiber filter cake into it to form an edible and medicinal fungus fiber filter cake embedded with a sensing chip, place it in a paper sheet former, shape it, dehydrate and dry it, and obtain edible and medicinal fungus fiber-based sensing chip embedded paper.

2. The method for preparing a paper embedded with a sensor chip based on edible and medicinal fungi fiber as claimed in claim 1, characterized in that: The edible and medicinal fungus in step 1 is selected from any one of Ganoderma lucidum, Phellinus linteus, Lentinus edodes, Auricularia auricula, Hericium erinaceus, Grifola frondosa, and Poria cocos.

3. The method for preparing a paper embedded with a sensor chip based on edible and medicinal fungi fiber as claimed in claim 2, characterized in that: The preparation method of the solid culture medium in step 1 is as follows: put the solid culture matrix raw material into a cultivation bag, punch a small hole in the center of the bag body of the cultivation bag; sterilize at 115-121° C. for 20-120 min; wait for the solid culture matrix to cool to 25-30° C. to obtain a solid culture medium.

4. The method for preparing a paper embedded with a sensor chip based on edible and medicinal fungi fiber as claimed in claim 3, characterized in that: The solid culture medium in step 1 comprises solid culture matrix raw materials and water, wherein the solid culture matrix raw materials comprise: 70% to 78% sawdust, 20% to 26% bran, 1% to 2% gypsum, and 1% to 2% sugar.

5. The method for preparing a paper embedded with a sensor chip based on edible and medicinal fungi fiber as claimed in claim 4, characterized in that: After step 1, the method further comprises: using naked eye observation to screen out aerial hyphae of edible and medicinal fungi that are white in color, free of abnormal discoloration, and grow evenly and densely; and using scanning electron microscope observation to screen out aerial hyphae of edible and medicinal fungi that are thick in morphology and have a hyphae diameter of not less than 1 μm.

6. The method for preparing a paper embedded with an edible and medicinal fungus fiber-based sensor chip as claimed in claim 5, characterized in that: The specific method of the drying treatment in step 2 is: putting the aerial hyphae of the edible and medicinal fungi with the sensor chip into a constant temperature blast drying oven at a temperature of 45 to 65° C. and drying for 5 to 10 hours.

7. The method for preparing a paper embedded with a sensor chip based on edible and medicinal fungi fiber as claimed in claim 6, characterized in that: After step 2, the method further comprises: using a reduced pressure drying method to screen and dry the aerial hyphae of the edible and medicinal fungi with the sensor chip so that the moisture content does not exceed 4%.

8. The method for preparing a paper embedded with a sensor chip based on edible and medicinal fungi fiber as claimed in claim 7, characterized in that: The specific method for preparing the edible and medicinal fungus fiber in step 3 is: The induction chip with the surface covered with the edible and medicinal fungi aerial mycelium is taken out from the dried edible and medicinal fungi aerial mycelium with the induction chip for later use, and then the remaining dried edible and medicinal fungi aerial mycelium is ground with water to prepare edible and medicinal fungi fungal fiber liquid, and the edible and medicinal fungi fungal fiber liquid is placed in a 45-65° C. water bath, and stirred at a speed of 200-300 r / min for 60-120 min; then, the edible and medicinal fungi fungal fiber liquid is placed in a centrifuge, and centrifuged at 3500-4500 r / min for 10-15 min; the precipitate after centrifugation is rinsed with pure water, and then centrifuged at 3500-4500 r / min for 10-15 min, and the obtained precipitate is edible and medicinal fungi fungal fiber.

9. The method for preparing a paper embedded with an edible and medicinal fungus fiber-based sensor chip as claimed in claim 8, characterized in that: The specific method of grinding the remaining dried edible and medicinal fungus aerial mycelium with water in step 3 is as follows: putting the dried edible and medicinal fungus aerial mycelium into a pulper, adding purified water, and obtaining an edible and medicinal fungus aerial mycelium suspension, making the concentration of the edible and medicinal fungus aerial mycelium in the edible and medicinal fungus aerial mycelium suspension 40-60 g / 100 mL, setting the grinding disc gap distance to 1-5 mm, the fly cutter rotor power to 1.1 kW, the fly cutter speed to 1430-1470 r / min, grinding the edible and medicinal fungus aerial mycelium suspension for 5-15 min, and obtaining an edible and medicinal fungus fiber liquid.

10. The method for preparing a paper embedded with a sensor chip based on edible and medicinal fungi fiber as claimed in claim 9, characterized in that: After step 3, the method further comprises: using a laser confocal fluorescence microscope to screen the edible and medicinal fungi fibers to determine if the length is not less than 20 μm and the width is not more than 1 μm.

11. The method for preparing a paper embedded with a sensor chip based on edible and medicinal fungi fiber as claimed in claim 10, characterized in that: The specific preparation method of the edible and medicinal fungus fiber pulp in the step 4 is as follows: add water to the edible and medicinal fungus fiber to prepare an edible and medicinal fungus fiber suspension, add sodium carboxymethyl cellulose to obtain suspension 1, stir the suspension 1 at 45 to 65° C. and at a speed of 150 to 200 r / min for 15 to 45 minutes with a magnetic stirrer, then add cationic starch to obtain suspension 2, stir the suspension 2 at 45 to 65° C. and at a speed of 150 to 200 r / min for 15 to 45 minutes with a magnetic stirrer to obtain edible and medicinal fungus fiber pulp.

12. The method for preparing a paper embedded with a sensor chip based on edible and medicinal fungi fiber according to claim 11, characterized in that: In step 4, the concentration of edible and medicinal fungus fiber in the edible and medicinal fungus fiber suspension is 30-60 g / 100 mL, the concentration of sodium carboxymethyl cellulose in suspension 1 is 0.2-1.2 g / 100 mL, and the concentration of cationic starch in suspension 2 is 0.4-1.4 g / 100 mL.

13. The method for preparing a paper embedded with a sensor chip based on edible and medicinal fungi fiber as claimed in claim 12, characterized in that: After step 4, the method further comprises: using a potentiometer to screen the edible and medicinal fungus fiber pulp to ensure that the surface charge is not less than 8mV, and using a particle size analyzer to screen the edible and medicinal fungus fiber pulp to ensure that the average particle size is not less than 1mm.

14. The method for preparing a paper embedded with a sensor chip based on edible and medicinal fungi fiber according to claim 13, characterized in that: The concentration of edible and medicinal fungus fibers in the edible and medicinal fungus pulp suspension in step 5 is 0.7-1.4 g / 100 mL; the gram weight of the edible and medicinal fungus fiber filter cake embedded with the sensing chip in step 5 is 50-100 g / m2; the specific conditions for dehydration and drying in step 5 are: heating power 10-20 kw, drying temperature 40-60°C, and drying time 12-24 h.

15. The method for preparing a paper embedded with a sensor chip based on edible and medicinal fungi fiber as claimed in claim 14, characterized in that: After step 5, the method further includes: using a contact angle meter to screen the contact angle of the paper embedded with the edible and medicinal fungus fiber-based sensor chip with water to be not less than 79°; using a folding endurance meter to screen the paper embedded with the edible and medicinal fungus fiber-based sensor chip to have a sensing function that will not be affected by folding 100 times; using an electronic universal tester to screen the tensile strength of the paper embedded with the edible and medicinal fungus fiber-based sensor chip to be not less than 180 MPa; using a smoothness meter to screen the surface smoothness of the paper embedded with the edible and medicinal fungus fiber-based sensor chip to be not less than 600s; using a stiffness meter to screen the stiffness of the paper embedded with the edible and medicinal fungus fiber-based sensor chip to be not less than 0.1 mNm; using a glossiness meter to screen the glossiness of the paper embedded with the edible and medicinal fungus fiber-based sensor chip to be not less than 70%.

16. Edible and medicinal fungus fiber-based sensor chip embedded paper prepared by the preparation method according to any one of claims 1 to 15.

17. Use of the paper prepared by the preparation method according to any one of claims 1 to 15 or the paper according to claim 16 as packaging paper.

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