Reagent for detecting and collecting drugs in environment and use method of reagent

Abstract

The invention relates to the technical field of drug detection, in particular to an environment drug detection and collection reagent and a use method thereof.The environment drug detection and collection reagent comprises 0.05%-5% of a preservative, 0.5%-10% of a scale inhibitor, 0.1%-5% of a surfactant and a pH regulator; the pH value of the drug detection and collection reagent in the environment is 6.5-8.5; the detection and collection reagent for the drugs in the environment is wide in application range, and can be suitable for rapid collection and detection of the drugs such as morphine, methamphetamine, ketamine, cocaine, cannabis sativa, ecaloin, etomidate and dextromethorphan. The drug detection and collection reagent can effectively reduce the detection threshold value of a high-sensitivity detection plate, further improves the sensitivity of drug detection and analysis, and is suitable for further popularization and application.

Description

Technical Field

[0001] The present invention relates to the technical field of drug detection, and in particular to a drug detection and collection reagent in an environment and a method for using the reagent. Background Art

[0002] At present, relevant departments can grasp the local drug situation through sewage drug testing, and finally trace the source to the building that discharges toxic sewage through rapid testing equipment. How to accurately locate the drug-related room without alerting the drug-related personnel has always been a long-standing problem for drug control personnel. Therefore, further locating the specific drug-related residents is an urgent problem to be solved.

[0003] To this end, the present invention designs a drug detection collection reagent in the environment and a method for using the reagent, which can achieve rapid detection by collecting trace targets on the surfaces of objects that can be touched in the living environment, such as elevator buttons and door handles in buildings. Rapid drug trace detection can be carried out on the collected environmental samples on site, and drug-related residents can be quickly located through on-site detection results. It assists case handlers in quickly detecting and screening target places, improving case handling efficiency and detection accuracy. Summary of the invention

[0004] Based on the above, the object of the present invention is to provide a drug detection and collection reagent in the environment and a method for using the same.

[0005] To achieve the above objectives, the present invention provides a reagent for detecting and collecting drugs in the environment, comprising: a preservative, an antiscalant, a surfactant and a pH regulator.

[0006] As a possible implementation manner, further, the drug detection and collection reagent in the environment includes: 0.05%-5% preservative, 0.5%-10% scale inhibitor, 0.1%-5% surfactant and pH adjuster; the pH of the drug detection and collection reagent in the environment is 6.5-8.5.

[0007] As a possible implementation manner, further, the preservative is Proclin-300.

[0008] As a possible implementation manner, further, the antiscalant is P300.

[0009] As a possible implementation manner, further, the surfactant is any one of TritonX-100, PEG4000, PVP-K30, disodium EDTA, Tween-20, S9, CTAB, S16, or a combination of at least two thereof.

[0010] As a possible implementation manner, further, the pH adjuster is any one of 10-200 mM sodium chloride and 50-300 mM phosphate buffer or a combination of two thereof.

[0011] The present invention also provides a method for using a reagent for detecting and collecting drugs in the environment, comprising the following steps:

[0012] 1) Number the reagent tubes containing the above-mentioned reagent for detecting and collecting drugs in the environment;

[0013] 2) Immerse the environmental collection swab in the collection reagent for soaking;

[0014] 3) Wipe the area to be tested with the wet environmental collection swab;

[0015] 4) Put the wiped environmental collection swab into the reagent tube containing the collection reagent, fold the wiped part of the environmental collection swab and leave it in the tube, cover the reagent tube cap and shake well to obtain a sample release solution;

[0016] 5) Squeeze 1 to 2 drops of the numbered sample release solution into the sample addition hole of the corresponding high-sensitivity detection plate, and directly observe with the naked eye to check the test result.

[0017] As a possible implementation manner, further, the method for using the reagent for detecting and collecting drugs in the environment further includes: 6) Make a record of the detection result and conduct a retest in the laboratory.

[0018] The reagent for detecting and collecting drugs in the environment provided by the present invention can be used in actual application scenarios such as on-site inspections in entertainment places such as hotels, barbecue stalls, Internet cafes, etc., household surveys in key communities, random inspections of fishing boats, customs inspections, and investigations of drug manufacturing sites.

[0019] The reagent for detecting and collecting drugs in the environment provided by the present invention has a wide range of applications and can be used for rapid collection and detection of drugs such as morphine, methamphetamine, ketamine, cocaine, cannabis, ecstasy, etomidate, dextromethorphan, etc. Moreover, the detection threshold of this drug detection and collection reagent is low, which further improves the sensitivity of the high-sensitivity detection plate for drug detection and analysis, and is suitable for further popularization and application. Description of the Drawings

[0020] Figure 1 It is a schematic diagram of the results of dripping the same concentration of drug solutions prepared with the special collection reagent (1-1) and pure water (1-2) provided by the present invention onto the high-sensitivity detection plate respectively; in the figure, the negative result of the high-sensitivity detection plate shows two lines, and the positive result shows one line. Detailed Embodiments

[0021] The following embodiments facilitate a better understanding of the present invention, but do not limit the present invention. The experimental methods and apparatuses in the following embodiments are conventional methods and apparatuses unless otherwise specified. The test materials used in the following embodiments are purchased from conventional biochemical reagent companies unless otherwise specified. To make the objectives, technical solutions, and advantages of the present invention clearer, the specific embodiments of the present invention will be described in detail below in conjunction with specific embodiments. Examples of these preferred embodiments are illustrated in the specific embodiments.

[0022] Here, it should also be noted that in order to avoid obscuring the technical solution of the present invention due to unnecessary details, only the technical solutions and / or processing steps closely related to the solution of the present invention are shown in the embodiments, while other details with little relevance are omitted.

[0023] This embodiment provides an environmental drug detection and collection reagent, including: 0.05%-5% preservative, 0.5%-10% scale inhibitor, 0.1%-5% surfactant, pH regulator; the pH of the collection reagent is 6.5-8.5.

[0024] Among them, the preservative is Proclin-300, and its main function is to inhibit the contamination and degradation of the reagent by microorganisms during storage and reaction after environmental collection, while extending the shelf life of the reagent and ensuring the stable performance of the reagent.

[0025] The scale inhibitor is P300, which can prevent the formation of scale or other precipitates in the reagent, maintain the clarity and stability of the reagent. The scale inhibitor combines with calcium, magnesium and other ions in water to form stable complexes, thereby preventing the formation of scale.

[0026] The surfactant is any one or a combination of at least two of TritonX-100, PEG4000, PVP-K30, disodium EDTA, Tween-20, S9, CTAB, S16; the surfactant can reduce the surface tension of the reagent, increase the contact area and permeability between the reagent and the environmental sample, which helps to better collect the target substances in the environment and improve the sensitivity and accuracy of the analysis.

[0027] The pH regulator is any one or a combination of two of 10-200 mM sodium chloride and 50-300 mM phosphate buffer. The pH of the adjusted collection reagent is 6.5-8.5. To adapt to different environmental collection requirements and analysis methods. Different environmental samples may have different pH values, and the high-sensitivity detection plate also has specific requirements for the pH value of the reagent. By using the pH regulator, the pH value of the reagent can be adjusted to an appropriate range to ensure the reliability of the analysis results.

[0028] This embodiment also provides a method for using a reagent for detecting and collecting drugs in the environment, including the following steps:

[0029] 1) Number the reagent tubes containing the above-mentioned reagent for detecting and collecting drugs in the environment (the required time is about 5 seconds);

[0030] 2) Immerse the environmental collection swab in the collection reagent for 10 seconds;

[0031] 3) Wipe the area to be tested with the wet environmental collection swab; try to wipe all areas as much as possible (the required time is about 20 seconds);

[0032] 4) Put the wiped environmental collection swab into the reagent tube containing the collection reagent, fold the wiped part of the environmental collection swab and leave it in the tube, cover the reagent tube cap and shake well for 10 - 20 seconds to obtain a sample release solution;

[0033] 5) Squeeze 1 - 2 drops of the numbered sample release solution into the sample addition hole of the corresponding high - sensitivity detection plate (the required time is 10 seconds); after reacting for 5 minutes, directly observe with the naked eye to check the test result.

[0034] 6) Record the test results and conduct a retest in the laboratory.

[0035] In summary, the time spent on the detection is 30 seconds for sample extraction + 5 minutes for detection. Compared with the existing method of detecting drugs in sewage, this method can complete the corresponding detection within 10 minutes without disturbing the residents, and can quickly conduct a corresponding drug situation investigation on the suspected building.

[0036] The high - sensitivity detection plate used in this embodiment is a drug high - sensitivity detection plate product developed by our company, which has been put on the market and is an existing product; it can be assembled according to the drug situation in different urban areas and can achieve rapid screening of drugs such as morphine, methamphetamine, ketamine, cocaine, cannabis, ecstasy, etomidate, dextromethorphan, etc.

[0037] The product models are as follows:

[0038]

[0039] Performance test

[0040] Refer to the appendix Figure 1 As shown, prepare drug solutions with the same concentration using the special collection reagent (1 - 1) and pure water (1 - 2) provided by the present invention, and drop them into the high - sensitivity detection plate for detection respectively; it can be seen from the test results that the detection threshold of the high - sensitivity detection plate dropped by using the special collection reagent provided by the present invention is lower.

[0041] Through experiments, when using the special collection reagent provided by the present invention to drop the high - sensitivity detection plate, the detection thresholds of each drug are as follows:

[0042] Morphine - 10 ng / mL; Methamphetamine - 20 ng / mL; Ketamine - 100 ng / mL; Etomidate - 100 ng / mL; Cannabis - 50 ng / mL; Ecstasy - 50 ng / mL; Cocaine - 50 ng / mL.

[0043] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A drug detection and collection reagent in the environment, characterized in that: include: Preservatives, scale inhibitors, surfactants and pH adjusters.

2. The drug detection and collection reagent in the environment according to claim 1, characterized in that: include: 0.05%-5% preservative, 0.5%-10% antiscalant, 0.1%-5% surfactant and pH regulator; The pH of the drug detection and collection reagent in the environment is 6.5-8.

5.

3. The drug detection and collection reagent in the environment according to claim 1, characterized in that: The preservative is Proclin-300.

4. The drug detection and collection reagent in the environment according to claim 1, characterized in that: The antiscalant is P300.

5. The drug detection and collection reagent in the environment according to claim 1, characterized in that: The surfactant is any one of TritonX-100, PEG4000, PVP-K30, disodium EDTA, Tween-20, S9, CTAB, and S16, or a combination of at least two thereof.

6. The drug detection and collection reagent in the environment according to claim 1, characterized in that: The pH regulator is any one of 10-200 mM sodium chloride and 50-300 mM phosphate buffer or a combination of the two.

7. A method for using a drug detection and collection reagent in an environment, characterized in that: The steps include: 1) Numbering the reagent tubes containing the environmental drug detection and collection reagent according to any one of claims 1 to 6; 2) immersing the environmental collection swab in the collection reagent for soaking; 3) Wipe the area to be tested with a moist environmental collection swab; 4) Place the wiped environmental collection swab into a reagent tube containing a collection reagent, fold down the wiped part of the environmental collection swab and leave it in the tube, cover the reagent tube cap and shake well to obtain a sample release solution; 5) Squeeze 1 to 2 drops of the numbered sample release solution into the corresponding sample well of the high-sensitivity detection plate, and observe directly with the naked eye to check the test results.

8. The method for using the drug detection and collection reagent in the environment according to claim 7, characterized in that: Also includes: 6) Record the test results and conduct laboratory retesting.

9. An application of a drug detection and collection reagent in an environment as claimed in any one of claims 1 to 6, characterized in that: The drug detection and collection reagents in the described environment can be used in spot checks at entertainment venues, basic screening of key communities, random inspections of fishing vessels, customs inspections, and investigations of drug manufacturing sites.

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