Fungus cell wall resistance-reducing feed additive and application thereof in promoting improvement of animal growth performance

Abstract

The invention discloses a fungal cell wall resistance-reducing feed additive and application thereof in promoting improvement of animal growth performance. The fungal cell wall resistance-reducing feed additive can efficiently replace antibiotics, has no residual risk, remarkably improves animal production performance, enhances anti-stress ability, maintains stable production, can also optimize intestinal health and digestion and absorption, and is natural and safe in components and wide in applicability.

Description

Technical Field

[0001] The present invention relates to a fungal cell wall antibiotic-reducing feed additive and its application in promoting the improvement of animal growth performance, belonging to the field of application of fungal cell wall antibiotic-reducing feed additives. Background Art

[0002] The "fungal cell wall antibiotic-reducing feed additive" is a natural and residue-free feed additive for promoting the growth of livestock and poultry. Its main components are composed of various effective components such as fungal cell wall β-1,3-β1,6-D-Glucans and beneficial microorganisms. It can promote the improvement of animal production performance, enhance animal immunity, replace antibiotics, improve the fermentation effect of livestock and poultry intestines and promote the reproduction of beneficial bacteria, and reduce the impact of stress, etc. Summary of the Invention

[0003] Object of the Invention: The technical problem to be solved by the present invention is to provide a fungal cell wall antibiotic-reducing feed additive and its application in promoting the improvement of animal growth performance.

[0004] Technical Solution: To solve the above technical problem, the present invention provides the application of a fungal cell wall antibiotic-reducing feed additive in preparing an additive composition for relieving animal heat stress.

[0005] The present invention also provides the application of a fungal cell wall antibiotic-reducing feed additive in improving animal production performance.

[0006] Wherein, the application further includes increasing the feed intake of dry matter by animals.

[0007] Wherein, the application further includes improving the digestibility of feed by animals.

[0008] Wherein, when the animal is a dairy cow, the application further includes increasing the daily milk production of dairy cows.

[0009] The present invention also provides the application of a fungal cell wall antibiotic-reducing feed additive in promoting animal growth.

[0010] The present invention also provides the application of a fungal cell wall antibiotic-reducing feed additive in preparing an additive composition for reducing the feed-to-meat ratio of animals.

[0011] Wherein, the animals include pigs.

[0012] Advantageous Effects: Compared with the prior art, the present invention has the following remarkable advantages:

[0013] 1. Highly efficient in replacing antibiotics, without the risk of residue

[0014] The present invention uses fungal cell wall extract (β1,3-β1,6-D-glucan) as the core ingredient, combined with beneficial microorganisms and their metabolites, to completely replace the use of antibiotics. Tests have shown that in dairy cows and nursery pigs, this additive significantly improves production performance, and without relying on antibiotics, it avoids drug residues and drug resistance problems, meeting the requirements of food safety and environmental protection.

[0015] 2. Significantly improve animal production performance

[0016] Dairy cows: In the experimental group under heat stress environment (THI > 72), the daily milk production increased by 3.27 kg (in July) and 1.7 kg (in August) compared with the control group, the dry matter intake increased by 2.74 kg / day, and the proportion of fecal digestibility in the lower layer reached 72% (only 48% in the control group), indicating that the feed utilization rate was significantly optimized.

[0017] Nursery pigs: The total weight gain of the experimental group was 40 - 72 kg higher than that of the control group at 66 days of age, the feed-to-meat ratio decreased to 1.28 - 1.33 (1.55 - 1.61 in the control group), and the feed conversion efficiency increased by more than 20%, significantly reducing the breeding cost.

[0018] 3. Enhance stress resistance and maintain stable production

[0019] Under the condition of heat stress in dairy cows (THI 80 - 85), the respiratory rate of the experimental group decreased significantly (44.23 times / min vs. 52.26 times / min in the control group), and the rectal temperature was more stable, effectively alleviating the negative impact of heat stress on production performance. Under complex environments such as high temperature and epidemic situations, the milk production and growth performance can still be maintained stably.

[0020] 4. Optimize intestinal health and digestion and absorption

[0021] By promoting the reproduction of beneficial bacteria and inhibiting pathogenic bacteria, the residual proportion in the lower layer of the dairy cow fecal sieve test increased to 66% - 72% (only 38% - 48% in the control group), indicating that the feed digestibility was significantly improved. The fecal excretion situation of nursery pigs improved, and the intestinal fermentation effect was enhanced, further reducing the diarrhea rate and disease risk.

[0022] 5. Natural and safe, with wide applicability

[0023] The ingredients are all natural, without chemical synthetic additives, and there are no side effects after long-term use. The tests cover dairy cows and nursery pigs, proving its universality for ruminants and monogastric animals, and it can be extended to the entire field of livestock and poultry, with flexible application scenarios.

[0024] 6. Significant economic benefits

[0025] By increasing milk production, reducing the feed-to-meat ratio (the feed cost for nursery pigs is reduced by more than 20%), and reducing the cost of antibiotic use, the comprehensive economic benefits are significantly improved, providing a sustainable solution for large-scale farming.

[0026] Compared with traditional antibiotics or single probiotic additives, the present invention shows more comprehensive performance in aspects such as stress resistance, growth promotion, and intestinal health through the synergistic action of multiple components (β-glucan + microbial metabolites), and there is no risk of drug resistance. Its comprehensive performance is significantly better than similar products in the market. Description of the Drawings

[0027] Figure 1 It is the change curve of THI in the cowshed;

[0028] Figure 2 It is the change curve of dry matter intake of dairy cows;

[0029] Figure 3 It is the change curve of daily milk production of dairy cows in July;

[0030] Figure 4 It is the separation result of the cattle herd feces. Detailed Embodiments

[0031] The technical solution of the present invention will be further described below in conjunction with the drawings.

[0032] Example 1

[0033] The experiment of the present invention was carried out in a ranch.

[0034] 1. Selection, grouping and experiment of test animals

[0035] (1) Select 120 healthy Holstein dairy cows in the lactation period (30 - 159 days) in this cattle farm, and divide them into two groups, namely the control group and the experimental group, with 60 cows in each group according to the principle of similar age, parity, lactation days, and daily milk production.

[0036] (2) Transition method for the experimental group: The experimental group was pre-fed and transitioned for 7 days, with an additional 2 g / day / head added every day, and gradually added the fungal cell wall antibiotic-reducing feed additive to the test period dose. That is, 2 g / day / head was added on the first day, 4 g / day / head on the second day, 6 g / day / head on the third day,... 15 g / day / head on the seventh day.

[0037] (3) Formal test: In the experimental group, the fungal cell wall antibiotic-reducing feed additive was added at a rate of 15 g / head / day and fed continuously. Note that the fungal cell wall antibiotic-reducing feed additive needs to be premixed with the concentrate feed and then added to the total mixed ration (TMR, Table 1) for stirring to ensure the uniformity of the fungal cell wall antibiotic-reducing feed additive in the TMR, so that each cow can eat equally. It is fed three times a day. The control group did not add the fungal cell wall antibiotic-reducing feed additive, and the rest was the same as the experimental group. The diet composition, feeding amount and feeding method of the test cows were the same. Among them, the proportion of the additive components was as follows: microbial flora (13-15%, containing Lactobacillus acidophilus, Lactobacillus casei, yeast cells, not less than 5×10 9 CFU / g), mannan oligosaccharide (MOS, 6-7%), β-glucan (1-2%), carrier and coating material (76-80%).

[0038] Table 1 Composition and nutritional level of the basic diet for dairy cows (dry matter basis)

[0039]

[0040] Note: The mass ratio of concentrate feed to roughage during the lactation period is 50:50 (peak lactation) to 40:60 (mid-late stage). The total dietary dry matter is about 20-25 kg / head / day. The values in the table are converted to the actual feeding amount according to 60% of the dry matter. The concentrate-roughage ratio can be adjusted according to the cow's body weight, milk yield and stage.

[0041] 2. Test indicators and methods

[0042] Production performance: Daily dry matter intake of TMR (obtained from the daily feeding record sheet of the TMR mixing workshop or the precise feeding system and the corresponding number of cows) and daily milk yield per cow (obtained from the automatic milk measurement system in the milking hall).

[0043] Thermohygrometers were installed at a height of 1.5 m from the ground in the middle of the feed trough of each group of cows, and the temperature and humidity in the cowshed were recorded at fixed times every day (10:00, 14:00, 18:00). The formula is as follows:

[0044] THI = 0.81T + (0.99T - 14.3)R + 46.3

[0045] In the formula: T is the temperature (°C); R is the relative humidity:

[0046] 3. Test results

[0047] (1) Temperature-humidity index in the cowshed

[0048] It is reported that when THI is less than 72, dairy cows are in a non-stressed state; when THI is 72-79, dairy cows are in a mild heat stress state; 79-88 is a moderate heat stress state; and greater than 89 is a severe heat stress state. From Figure 1It can be seen that during the entire experimental period, the THI at noon was greater than 72, and the dairy cows were in a state of heat stress. From the 19th to the 49th day of the experiment, the THI was between 80 and 85, and the dairy cows were in a state of moderate heat stress.

[0049] (2) Rectal temperature and respiratory rate of dairy cows

[0050] Table 2 Effects of adding a fungal cell wall antibiotic-reducing feed additive on the rectal temperature and respiratory rate of dairy cows

[0051]

[0052] As can be analyzed from Table 1, after adding the fungal cell wall antibiotic-reducing feed additive, the respiratory rates of dairy cows in the morning and at noon were significantly lower than those of the control group (p < 0.05). Generally in summer, the respiratory rate of dairy cows under heat stress will increase significantly. This experiment shows that adding the fungal cell wall antibiotic-reducing feed additive reduces the respiratory rate of dairy cows, which is beneficial to alleviating the heat stress of dairy cows.

[0053] (3) Dry matter intake

[0054] As Figure 2 shown, from the 48th day to the end of the experiment, the average feed intake of the experimental group became 26.05 kg, while that of the control group was 23.31 kg. Therefore, being in a state of heat stress for a long time caused the feed intake of the control group of dairy cows to decrease; while using the fungal cell wall antibiotic-reducing feed additive significantly increased the dry matter intake of dairy cows.

[0055] (4) Average daily milk yield

[0056] As Figure 3 shown, in July, the average daily milk yield of the experimental group of first-calving cows was about 3.27 kg higher than that of the control group (the first 7 days were the trial period).

[0057] (5) Fecal sieve test

[0058] Use a fecal sieve (Cargill fecal separator sieve) to analyze the proportion of fecal residues in the upper, middle, and lower layers.

[0059] Table 3 Effects of adding a fungal cell wall antibiotic-reducing feed additive on the digestibility of dairy cows

[0060]

[0061] As shown in Table 2, from July 2 (the 7th day of the preliminary trial period) to the end of the experiment, the proportion of the third layer of the fecal sieve in the experimental group was 66% - 72%, while that in the control group was 38% - 48%. The experimental group was significantly better than the control group. This shows that after using the fungal cell wall antibiotic-reducing feed additive, the digestibility of dairy cows for feed is much higher than that of the control group.

[0062] The target ratios for the upper, middle, and lower layers of the fecal sieve are <10%, <20%, and >50%. As Figure 4 shown, starting from the 7th day of using the fungal cell wall antibiotic-reducing feed additive, the upper layer reached the ideal value; and on the 30th day, the digestibility of cows could be effectively improved, making the upper, middle, and lower layers all reach the target values, especially the lower layer reaching as high as 72%.

[0063] 4. Conclusions

[0064] This experiment showed that during the entire experimental period, the THI at noon was greater than 72, and the dairy cows were in a state of heat stress; while adding the fungal cell wall antibiotic-reducing feed additive significantly reduced the respiratory rate of the dairy cows and alleviated their heat stress. From the 48th day to the end of the experiment, the fungal cell wall antibiotic-reducing feed additive increased the average dry matter intake of the dairy cows by about 2.74 kg. For milk production, in July, the experimental group was about 3.27 kg higher than the control group, and in August, it was still about 1.7 kg higher. The improvement effect of the fungal cell wall antibiotic-reducing feed additive on the digestibility of dairy cows was very significant. Starting from the 7th day of the experiment, the digestibility was close to the ideal value; and on the 30th day, the upper, middle, and lower layers of the fecal sieve test all reached the target values, especially the lower layer reaching as high as 72%.

[0065] Example 2

[0066] 1. Experiment time and location

[0067] The experiment was carried out in the nursery house, with the first 5 days being the transition period.

[0068] 2. Materials and methods

[0069] (1) Experimental animals

[0070] In this experiment of the present invention, 80 crossbred (Large White, Landrace) male piglets with a body weight of about 7 kg were selected.

[0071] (2) Experimental diets

[0072] The experimental diets were self-prepared diets. The experimental group was added with 1 kg / ton of the fungal cell wall antibiotic-reducing feed additive, and the control group was not added. The diet formulas and nutritional levels of each group are shown in Table 4. The feed consumption was recorded separately by the method of emptying the trough.

[0073] Table 4 Feeding amount of the basic diet for piglets (g / head / day)

[0074]

[0075] Note: The total feeding amount of the piglet diet is about 2,400 - 2,700 g / head / day, and it needs to be adjusted according to the body weight and growth requirements specifically.

[0076] (3) Experimental methods

[0077] The experiment of this invention lasted for 44 days in total. The piglets were weighed once at 42 days old and once at 65 days old. The experiment was designed with two experimental groups, two control groups, 4 pens, and 20 piglets in each pen.

[0078] 3 Precautions

[0079] (1) Try not to change the formula during the experiment. If fine-tuning is needed, make sure that the experimental group is consistent with the control group.

[0080] (2) The experimental environment and various operations of the experimental group and the control group are the same.

[0081] (3) If diseases are found during the experiment, isolate them in time and record the pig numbers.

[0082] (4) Record the immunization situation of the pig herd in detail.

[0083] 4. Experimental data:

[0084] Table 5 Weights of pigs at different ages

[0085]

[0086] Table 6 Feed conversion ratios of pigs at different ages

[0087]

[0088] Note: All data are in kg, and the weights of dead pigs have been included in the weight.

[0089] The experimental groups showed (Tables 3 and 4) that the feed conversion ratios were 1.31 and 1.33 respectively, much lower than those of the control group. Conclusion: It shows that the fungal cell wall anti - antibiotic feed additive has an obvious growth - promoting effect.

Claims

1. Application of fungal cell wall antibiotic-reducing feed additive in the preparation of additive composition for alleviating animal heat stress.

2. Application of fungal cell wall antibiotic-reducing feed additives in improving animal production performance.

3. The application according to claim 2, characterized in that: The use also includes increasing the dry matter intake of animals.

4. The application according to claim 2, characterized in that: The application also includes improving the digestibility of feed for animals.

5. The use according to any one of claims 1 to 4, characterized in that: The animal includes a cow or a pig.

6. The use according to claim 1, characterized in that: When the animal is a dairy cow, the application also includes increasing the daily milk production of the dairy cow.

7. Application of fungal cell wall antibiotic-reducing feed additives in promoting animal growth.

8. Application of fungal cell wall antibiotic-reducing feed additive in the preparation of additive composition for reducing animal feed-to-meat ratio.

9. The use according to claim 7 or 8, characterized in that: The animals include pigs.

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