Full-automatic disinfection kit and gene sequencing pretreatment equipment

Abstract

The invention provides a full-automatic sterilization kit and gene sequencing pretreatment equipment, and relates to the technical field of gene sequencing pretreatment, the full-automatic sterilization kit comprises an atomization head, a kit body and a kit cover, the atomization head is in fluid communication with the kit body, the kit cover is arranged at the upper end of the kit body, and the atomization head is in fluid communication with the kit body. A power supply connecting part and a butt joint part are arranged on the upper side of the kit cover, the power supply connecting part is electrically connected with the atomization head, the butt joint part is used for being in butt joint with a driving part to drive the full-automatic disinfection kit to move, and an atomization outlet communicated with the interior of the kit body is further formed in the kit body and / or the kit cover. According to the technical scheme, the technical problems that in the prior art, gene sequencing pretreatment equipment is low in internal sterilization efficiency and cannot completely sterilize in all directions are solved.

Description

Technical Field

[0001] This application relates to the technical field of pre-treatment for gene sequencing, in particular to a fully automatic disinfection and sterilization kit and a pre-treatment device for gene sequencing. Background Art

[0002] In a pipetting workstation, after the nucleic acid extraction and library construction processes are completed, nucleic acid fragments caused by residual aerosol or splashing will remain on the inner wall of the instrument. If these nucleic acid fragments are not processed, it is highly likely that they will contaminate new batches of samples in the next experiment, thus affecting the accuracy of the sequencing results. Currently, the general method for disinfecting and sterilizing residual nucleic acids is manual wiping, that is, dipping a wiping cloth in a nucleic acid removal reagent and then manually wiping the inner wall of the instrument with the wiping cloth, or spraying the nucleic acid removal reagent on the inner wall of the instrument with a manual sprayer and then drying it with a wiping cloth. This method is inefficient, has a high labor intensity, and some dead corners cannot be wiped, so complete disinfection and sterilization cannot be achieved. Summary of the Invention

[0003] This application provides a fully automatic disinfection and sterilization kit and a pre-treatment device for gene sequencing to solve the technical problems of low internal disinfection and sterilization efficiency and inability to achieve full and comprehensive disinfection and sterilization in the prior art pre-treatment device for gene sequencing. The many technical effects that can be produced by the preferred technical solutions among the many technical solutions provided in this application are described in detail below.

[0004] To achieve the above object, on the one hand, this application provides a fully automatic disinfection and sterilization kit, including: an atomizing head, a kit body, and a kit cover. The atomizing head is in fluid communication with the kit body. The kit cover is provided at the upper end of the kit body. A power connection part and a docking part are provided on the upper side of the kit cover. The power connection part is electrically connected to the atomizing head. The docking part is used to dock with a driving component to drive the movement of the fully automatic disinfection and sterilization kit. An atomizing outlet communicating with the inside of the kit body is also provided on the kit body and / or the kit cover.

[0005] Optionally, a partition is provided inside the bottom of the kit body. Through holes are provided on the partition. The atomizing head passes through the through holes and is arranged on the partition and accommodated inside the kit body.

[0006] Optionally, the atomizing head is hermetically connected to the partition.

[0007] Optionally, the docking part is a docking hole or a docking post.

[0008] Optionally, the power connection part is a male power contact or a female power contact.

[0009] Optionally, a liquid injection hole is provided on the kit cover, and a plug cover is provided corresponding to the liquid injection hole.

[0010] Optionally, the outlet direction of the atomizing outlet is set downward.

[0011] On the other hand, the present application provides a gene sequencing pretreatment device, including a driving component and the full-automatic disinfection and sterilization kit as described in any one of the above. The driving component includes a driving assembly and a pipettor. The pipettor is arranged on the driving assembly, and the end of the pipettor is adapted to the power connection part and the docking part of the full-automatic disinfection and sterilization kit.

[0012] Optionally, a pipette tip adapter is arranged at the end of the pipettor. The pipette tip adapter is a docking post, and the docking post is inserted into the docking part of the full-automatic disinfection and sterilization kit to fix the full-automatic disinfection and sterilization kit to the driving component.

[0013] Optionally, a power female contact is arranged at the end of the pipettor. The power connection part of the full-automatic disinfection and sterilization kit is a power male contact, and the power female contact is electrically connected to the power male contact to supply power to the atomizing head of the full-automatic disinfection and sterilization kit.

[0014] Optionally, the gene sequencing pretreatment device further includes a kit holder, and the full-automatic disinfection and sterilization kit can be placed on the kit holder in a non-working state.

[0015] Optionally, the driving component is a library construction pipetting assembly or a quality control pipetting assembly.

[0016] Optionally, the driving assembly is a three-axis robotic arm.

[0017] To make the features and advantages of the present application more obvious and understandable, some embodiments are specifically given below and described in detail in conjunction with the accompanying drawings as follows. Description of the Drawings

[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present application. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0019] Figure 1 is a schematic diagram of the nucleic acid extraction process provided by the embodiment of the present application;

[0020] Figure 2 is a schematic diagram of the structure of the gene sequencing pretreatment system in the embodiment of the present application;

[0021] Figure 3 is a schematic diagram of the structure of the quality control system in the embodiment of the present application;

[0022] Figure 4 It is a schematic structural diagram of the library construction system in the embodiment of the present application;

[0023] Figure 5 It is a schematic structural diagram of the electric control system in the embodiment of the present application;

[0024] Figure 6 It is a schematic structural diagram of a gene sequencing pretreatment device in the embodiment of the present application;

[0025] Figure 7 It is another schematic structural diagram of a gene sequencing pretreatment device in the embodiment of the present application;

[0026] Figure 8 It is a schematic structural diagram of a full-automatic disinfection and sterilization kit in the embodiment of the present application;

[0027] Figure 9 It is a schematic exploded view of the structure of a full-automatic disinfection and sterilization kit in the embodiment of the present application;

[0028] Figure 10 It is a schematic internal structure diagram of a full-automatic disinfection and sterilization kit in the embodiment of the present application:

[0029] Figure 11 It is a schematic structural diagram of the end part of a pipette in the embodiment of the present application:

[0030] Figure 12 It is a schematic structural diagram of the connection part between the pipette and the full-automatic disinfection and sterilization kit in the embodiment of the present application.

[0031] In the figure: 1. Gene sequencing pretreatment equipment; 1-1. Disinfection and sterilization kit board position; 1-2. Three-axis robotic arm; 2. Automatic disinfection and sterilization kit; 2-1. Ultrasonic atomizing head; 2-2. Kit body; 2-3. Kit cover; 2-4. Power connection part; 2-5. Plug cover; 2-6. Screw; 2-7. Docking part; 2-8. Liquid injection hole; 2-9. Partition board; 3. Pipettor; 3-1. Power supply female contact; 3-2. Pipette tip adapter; 10. Nucleic acid extraction system; 20. Library construction system; 21. PCR instrument; 22. Actual container for document construction; 23. Oscillation heating component; 24. Magnet assembly; 25. Working area of the piercing component; 26. Waste container; 27. Large-capacity reagent container; 30. Quality control system; 31. Quantitative analyzer; 32. Quality control operator component; 33. Conveyor mechanism; 34. Quality control pipetting component; 35. Construction reaction container; 36. Quality control container; 37. Detection reagent container; 40. Electric control system; 41. Central control module; 42. Nucleic acid extraction system control module; 420. Temperature control module; 421. Movement control module of the nucleic acid extraction platform; 422. Movement control module of the magnetic rod / magnetic rod sleeve; 43. Library construction system control module; 430. Movement control module of the library construction pipetting component; 431. Movement control module of the library construction operator component; 432. Oscillation heating component control module; 433. Refrigeration control module of the reagent container; 434. Refrigeration control module of the reagent addition area; 44. Quality control system control module; 440. Movement control module of the quality control operator component; 441. Movement control module of the conveyor mechanism; 442. Movement control module of the quality control pipetting component; 443. Quantitative analyzer control module; 45. Auxiliary component control module; 450. Camera control module; 451. Movement control module of the piercing component; 452. Ultraviolet lamp and other light source control module; 453. Air cleaning control module; 50. Host computer. Detailed implementation manners

[0032] To make the objectives, technical solutions and advantages of the present application clearer, the technical solutions of the present application will be described in detail below. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other implementation manners obtained by those of ordinary skill in the art without creative efforts shall fall within the scope protected by the present application.

[0033] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it can be directly on the other element or there may be an intermediate element at the same time. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or there may be an intermediate element at the same time.

[0034] Figure 1 Schematic diagram of the nucleic acid extraction process. AsFigure 1 As shown, the nucleic acid extraction provided by the embodiments of the present application is a technology for separating nucleic acids from biological samples by physical, chemical, biological methods or a combination of the above methods. It is mainly used to separate nucleic acid molecules (such as DNA or RNA) in biological samples from the biological samples for subsequent experimental analysis, such as sequencing analysis.

[0035] Taking the separation by chemical method as an example, by lysing the sample cells with a cell lysate, nucleic acid molecules can be released from the sample cells. The nucleic acid molecules and impurities such as proteins coexist in the lysate solution. If pure nucleic acid molecules are to be obtained, the lysate needs to be further purified. Taking the purification by magnetic bead method as an example, magnetic beads are added to the lysate. The nucleic acid molecules released from the sample cells are specifically adsorbed on the surface of the magnetic beads, while impurities such as proteins are not adsorbed and remain in the lysate. After reacting for a certain time, under the action of a magnetic field, the magnetic beads adsorbed with nucleic acid molecules are separated from the solution. Then, the magnetic beads adsorbed with nucleic acid molecules are washed with a washing solution to wash away impurities such as proteins. At the same time, for thorough washing, the washing can be repeated multiple times. The pure magnetic beads adsorbed with nucleic acid molecules are recovered. Finally, by eluting with an eluent, the adsorption between the nucleic acid molecules and the magnetic beads is released. After reacting for a certain time, again under the action of a magnetic field, the magnetic beads and the nucleic acid molecules are separated. After removing the magnetic beads, only pure nucleic acid molecules remain in the nucleic acid extraction product.

[0036] Furthermore, the embodiments of the present application provide quality control of nucleic acid extraction products. It is very important to detect the concentration (or perform concentration quantitative analysis) of nucleic acid molecules (such as DNA or RNA) in the nucleic acid extraction product solution. Usually, there are certain requirements for the total amount of nucleic acids required in the subsequent library construction process. Once the actual nucleic acid input amount exceeds the total amount of nucleic acids required in the subsequent library construction process, it will affect the amplification efficiency during library construction. Therefore, before library construction, it is also necessary to detect the nucleic acid concentration in the nucleic acid extraction product. After detecting the nucleic acid concentration, the total amount of the required nucleic acid extraction product can be further determined according to the nucleic acid concentration and the total amount of nucleic acids required in the subsequent library construction process. And when the detected nucleic acid concentration is too high, it will cause the total amount of the required nucleic acid extraction product to be lower than the minimum pipetting volume of the pipetting device and affect the pipetting accuracy. Therefore, it is necessary to appropriately dilute the nucleic acid extraction product with a diluent (such as pure water) before pipetting. Several common concentration detection methods include, for example, spectrophotometry, fluorescent dye detection method, microfluidic analysis method, or capillary gel electrophoresis method.

[0037] Furthermore, the embodiments of the present application provide library construction. In next-generation sequencing (NGS) technology, nucleic acid molecules (such as DNA or RNA) extracted from biological samples need to be fragmented physically or enzymatically. For example, they can be fragmented by ultrasonic waves. After fragmentation, nucleic acid fragments are formed. First, the ends of the nucleic acid fragments are filled in with enzymes, and then specific DNA sequences (usually referred to as adapters) are ligated to the ends of the fragments using specific enzymes. Finally, the formed nucleic acid fragments are called libraries in the industry.

[0038] To save sequencing costs, generally, multiple biological samples are sequenced simultaneously in a sequencer. To distinguish the sequencing results of different samples, when preparing libraries of different samples, a DNA sequence (usually containing 6 - 8 bases) that can identify the source of the sample is included in the adapter. This DNA sequence that can identify the source of the sample can also be called a sample tag (or Index, Barcode). It can be understood that each sample's library adapter contains its exclusive sample tag.

[0039] Furthermore, the embodiments of the present application provide quality control of library construction products. The quality of the constructed library is crucial for the quality of the data generated by subsequent gene sequencing. Therefore, before sequencing on the machine, it is also necessary to detect and control the quality of the library in the library construction products. Usually, library quality detection includes at least one of library length detection, library concentration detection, and library contaminant detection. Among them, through library concentration detection (or concentration quantitative analysis), the library concentration can be detected. When the detected library concentration is too high, it will cause the total amount of library construction products required for subsequent sequencing to be lower than the minimum pipetting volume of the pipetting device and affect the pipetting accuracy. Therefore, it is necessary to add a diluent (such as pure water) to the library construction products for appropriate dilution before pipetting. In addition, due to the differences in the library concentrations of multiple biological samples and the differences in the sequencing data output, the total amounts of library construction products of multiple biological samples required for subsequent sequencing are different, which ultimately affects the balance of the sequencing data output of multiple biological samples. Therefore, it is also necessary to add a diluent (such as pure water) to the library construction products of some biological samples for dilution before pipetting to narrow the gap between the total amounts of library construction products of multiple biological samples. Common concentration detection methods include, for example, spectrophotometry, fluorescent dye detection, microfluidic analysis, or capillary gel electrophoresis.

[0040] Here, it should be noted that for the quality control of library construction products and the quality control of nucleic acid extraction products, the concentration detection can use the same method or different methods respectively. The present application does not make any limitations in this regard.

[0041] Figure 2It is a schematic structural diagram of the gene sequencing pretreatment system in the embodiments of the present application. Further, the gene sequencing pretreatment system (or sequencing pretreatment instrument) provided by the embodiments of the present application includes: a nucleic acid extraction system 10, a library construction system 20, a quality control system 30, an electric control system 40, and a host computer 50. Among them:

[0042] The nucleic acid extraction system 10 is configured to controllably release nucleic acid molecules (such as DNA or RNA) from biological samples;

[0043] The library construction system 20 is configured to controllably prepare nucleic acid molecules (such as DNA or RNA) into libraries for on-machine sequencing;

[0044] The quality control system 30 is configured to controllably detect the concentration of nucleic acid molecules in the nucleic acid extraction products and adjust the concentration of nucleic acid molecules to a pre-required concentration range according to the detection results, and / or controllably detect the concentration of the libraries in the library construction products and adjust the concentration of the libraries to a pre-required concentration range according to the detection results;

[0045] The electric control system 40 is configured to control the operation and work of the nucleic acid extraction system 10, the library construction system 20, and the quality control system 30 according to the instructions of the host computer 50;

[0046] The host computer 50 is configured to control the operation and work of the nucleic acid extraction system 10, the library construction system 20, and the quality control system 30 by sending control instructions to the electric control system 40.

[0047] Among them, for the nucleic acid extraction system 10, taking chemical separation and magnetic bead purification as an example, the nucleic acid extraction system 10 at least includes a nucleic acid extraction platform and a magnetic rod / magnetic rod sleeve assembly. Among them, an extraction operation container (such as a single reagent strip or a deep well plate) and a heating device (such as a heating block) are arranged on the nucleic acid extraction platform. In addition to accommodating one or more reagents required for nucleic acid extraction (such as cell lysate, washing solution, elution solution), the extraction operation container can also accommodate biological samples and magnetic particles (such as magnetic beads), and provide a reaction space for various chemical reactions (such as lysis reaction, washing reaction, elution reaction) during nucleic acid extraction; the heating device can provide suitable reaction temperatures and reaction conditions for various chemical reactions occurring in the extraction operation container. The magnetic rod sleeve is sleeved on the magnetic rod to prevent the magnetic rod from directly contacting the nucleic acid extraction reagent and corroding the magnetic rod. When the magnetic rod / magnetic rod sleeve assembly enters the extraction operation container through the movement mechanism, it can provide a necessary magnetic field environment for the washing reaction and elution reaction during nucleic acid extraction.

[0048] It should be noted here that the nucleic acid extraction system 10 can batch-process nucleic acid extraction of multiple biological samples.

[0049] For the quality control system 30, such as Figure 3As shown, the quality control system 30 at least includes a quantitative analyzer 31, a quality control operating hand component 32 ( Figure 3 only its working area is shown), a conveying mechanism 33, a quality control pipetting component 34 ( Figure 3 only its working area is shown), a construction reaction container 35, a quality control container 36, and a detection reagent container 37. It should be noted that Figure 3 this is only schematic and should not be construed as a limitation on the positions of the respective parts.

[0050] Among them, the construction reaction container 35 (such as a microplate) can accommodate nucleic acid extraction products and library construction products, and further provide a reaction space for some chemical reactions during the library construction process; the quality control container 36 (such as a centrifuge tube) can accommodate the test substance and can be placed in the quantifier for detection; the detection reagent container 37 (such as a reagent kit) can accommodate one or more reagents required for the quantitative analyzer 31 to perform concentration detection and a diluent for adjusting the concentrations of nucleic acid extraction products and library construction products. The construction reaction container 35, the quality control container 36, and the detection reagent container 37 are respectively located in their respective storage areas, the quality control operating hand component 32 and the quality control pipetting component 34 work in their respective working areas, the storage area of the quality control container 36 is within the working area of the quality control operating hand component 32, and the storage areas of the construction reaction container 35 and the quality control reagent container 37 are within the working area of the quality control pipetting component 34. A conveying mechanism 33 is provided between the working area of the quality control operating hand component 32 and the working area of the quality control pipetting component 34, and the conveying mechanism 33 can reciprocally convey the quality control container 36 between the working area of the quality control operating hand component 32 and the working area of the quality control pipetting component 34.

[0051] Before the quality control starts, the quality control operating hand component 32 is responsible for taking out the quality control container 36 from the storage area of the quality control container 36 and placing it on the conveying mechanism 33. The conveying mechanism 33 conveys the quality control container 36 to the working area of the quality control pipetting component 34. The quality control pipetting component 34 is responsible for sucking a certain amount of detection reagent from the detection reagent container 37 and adding it to the quality control container 36. Then, the conveying mechanism 33 conveys the quality control container 36 (already added with the detection reagent) back to the working area of the quality control operating hand component 32, and the quality control operating hand component 32 is then responsible for putting the quality control container 36 (already added with the detection reagent) back into the storage area of the quality control container 36.

[0052] In addition, if it is the quality control of nucleic acid extraction products, the quality control pipetting component 34 is also responsible for sucking nucleic acid extraction products from the extraction operation container and adding them to the construction reaction container 35.

[0053] After the quality control starts, the quality control operator component 32 is responsible for taking out the quality control container 36 (already added with the detection reagent) from the storage area of the quality control container 36 and placing it on the transfer mechanism 33. The transfer mechanism 33 transfers the quality control container 36 (already added with the detection reagent) to the working area of the quality control pipetting component 34. The quality control pipetting component 34 is responsible for sucking a certain amount of nucleic acid extraction product / library construction product from the construction reaction container 35 and adding it to the quality control container 36, and then stirring it evenly. Then, the transfer mechanism 33 transfers the quality control container 36 (which has been added with the detection reagent and nucleic acid extraction product / library construction product successively) back to the working area of the quality control operator component 32. The quality control operator component 32 is then responsible for putting the quality control container 36 (which has been added with the detection reagent and nucleic acid extraction product / library construction product successively) back into the storage area of the quality control container 36.

[0054] After the detection reagent and nucleic acid extraction product / library construction product in the quality control container 36 react for a period of time, the quality control operator component 32 is responsible for taking out the quality control container 36 from the storage area of the quality control container 36 and putting it into the quantitative analyzer 31 for concentration detection. After the detection is completed, the quality control operator component 32 is then responsible for taking out the quality control container 36 and putting it back into the storage area.

[0055] If the detected nucleic acid molecule concentration / library concentration is higher than the required concentration, the quality control pipetting component 34 is also responsible for sucking a certain amount of pure water from the detection reagent container 37 and adding it to the construction reaction container 37 for dilution.

[0056] It should be noted here that the quality control system 30 can perform quality control processing on the nucleic acid extraction products / library construction products of multiple biological samples in batches. In the scenario of batch processing, the quality control operator component 32 can take and place the quality control containers 36 one by one in sequence, or can take and place multiple quality control containers 36 in one batch; correspondingly, the transfer mechanism 33 can transfer the quality control containers 36 one by one, or can transfer multiple quality control containers 36 in one batch; the quality control pipetting component 34 can be a single-channel pipetting component, sucking the detection reagent successively and adding it to the quality control container 36 successively, or can be a multi-channel pipetting component, sucking the detection reagent in one batch and adding it to multiple quality control containers 36 in one batch, and can be freely set according to the occupied space and working efficiency. Figure 2 Just for an example, in this example, the quality control operator component 32 takes and places the quality control containers 36 one by one. There are two container positions set on the transfer mechanism 33, and each can transfer one quality control container 36 in the opposite direction reciprocally. The quality control pipetting component 34 is a single-channel pipetting component.

[0057] In one implementable manner, the quality control pipetting assembly 34 can be a pipette (or a liquid transfer pump). To cooperate with the operation of the pipette, a pipette tip for liquid transfer also needs to be equipped for the pipette. When the liquid transfer operation starts, the pipette sleevs the pipette tip on the pipette tip adapter at its end. After the liquid transfer operation is completed, the pipette then detaches the pipette tip from the pipette tip adapter at its end. Correspondingly, the quality control system 30 is also provided with a pipette tip storage area, such as Figure 3 as shown

[0058] For the library construction system 20, as Figure 4 shown, the library construction system 20 at least includes a PCR instrument 21, a library construction manipulator assembly ( Figure 4 only its working area is shown in the figure), a library construction pipetting assembly ( Figure 4 only its working area is shown in the figure), a library construction reagent container 22, a shaking and heating assembly 23, and a magnet assembly 24. It should be noted that Figure 4 this is only schematic and should not be construed as a limitation on the positions of the respective parts

[0059] Among them, the library construction reagent container 22 (such as a reagent kit) can accommodate one or more reagents required for library construction. The library construction reagent container 22, the shaking and heating assembly 23, and the magnet assembly 24 are respectively located in their respective storage areas, and their respective storage areas are all within the working area ranges of the library construction manipulator assembly and the library construction pipetting assembly. The storage area of the library construction reagent container 22 generally needs to be in a low-temperature refrigerated environment to facilitate the low-temperature refrigeration of the library construction reagents. The shaking and heating assembly 23 is provided with a purification container (such as a deep well plate) and a shaking and heating device. The purification container can provide a reaction space for magnetic bead purification during library construction, and the shaking and heating device can provide suitable reaction temperatures and reaction conditions for magnetic bead purification. At the same time, the magnet assembly 24 can provide a necessary magnetic field environment for magnetic bead purification

[0060] After the nucleic acid extraction quality control is completed, the library construction operator component is responsible for taking out the construction reaction container 35 (already containing the nucleic acid extraction product) from the quality control system 30 and placing it in a specific storage area in the library construction system 20. In this storage area, various reaction reagents are added to the construction reaction container 35. For the convenience of description, this storage area can be called the reagent addition area. Since the nucleic acid is stored in the construction reaction container, the reagent addition area generally needs to be in a low-temperature refrigeration environment to facilitate the low-temperature refrigeration of the nucleic acid. Since the entire library construction process includes multiple reaction steps, for each reaction step, the library construction pipetting component is first responsible for sucking the corresponding reaction reagent from the library construction reagent container 22 and adding it to the construction reaction container 35 (already containing the nucleic acid extraction product). Then, the library construction operator component is responsible for putting the construction reaction container 35 (which has successively added the nucleic acid extraction product and the corresponding reaction reagent) into the PCR instrument 21 for reaction. After the reaction is completed, the library construction operator component takes out the construction reaction container 35 from the PCR instrument 21 and returns it to the specific area to wait for the addition of the reaction reagent for the next reaction, and repeats the above process.

[0061] Among them, during the library construction process, after adapters are ligated to both ends of the nucleic acid fragments, the ligation products need to be purified, and a pure library can be obtained after purification. In addition, since the amount of the library obtained at this time is small, library amplification is usually required, and the amplified products also need to be purified to obtain a pure library after amplification. If the magnetic bead method is used for purification, in addition to accommodating one or more reagents required for library construction (including the washing solution and elution solution required for magnetic bead purification) in the library construction reagent container 22, magnetic beads or other magnetic particles can also be accommodated. During the magnetic bead purification reaction of the ligation products or amplified products, the library construction pipetting assembly is responsible for first aspirating the ligation products / amplified products from the construction reaction container 35 and adding them to the purification container of the oscillating heating assembly 23, and then aspirating magnetic beads from the library construction reagent container 22 and adding them to this purification container (where the ligation products / amplified products have been added). After reacting for a certain period of time, the library construction manipulator assembly is responsible for transferring this purification container (the library has been adsorbed on the surface of the magnetic beads) to the magnet assembly 24. Under the action of the magnetic field, the magnetic beads adsorbed with the library are separated from the solution. The library construction pipetting assembly then aspirates the supernatant in this purification container, leaving the magnetic beads adsorbed with the library. The library construction pipetting assembly continues to aspirate the washing solution from the library construction reagent container 22 and add it to this purification container. After reacting for a certain period of time, the supernatant is aspirated away again. According to the experimental needs, the library construction pipetting assembly can be operated repeatedly for multiple washes. After the washing is completed, the library construction manipulator assembly transfers this purification container to the oscillating heating assembly 23. The library construction pipetting assembly continues to aspirate the elution solution from the library construction reagent container 22 and add it to this purification container. After reacting for a certain period of time, the adsorption between the library and the magnetic beads is released. The library construction pipetting assembly then aspirates the supernatant in this purification container (i.e., the library construction product) and adds it to the construction reaction container 35.

[0062] Generally, a sealing film is attached to the upper surface of the library construction reagent container 22 to facilitate the storage and transportation of the internal reagents. After the reagent container is loaded into the interior of the sequencing pretreatment instrument, the sealing film needs to be punctured by the puncturing assembly ( Figure 4 only its working area is shown in the figure) so that the corresponding pipetting assembly can aspirate the reagents from the library file reagent container 22, and the storage area of the library construction reagent container 22 is within the working area 25 of the puncturing assembly.

[0063] In addition, considering that the required amounts of magnetic beads, washing solution, and elution solution in the magnetic bead purification process are large and do not need to be stored in a low-temperature refrigerated environment, in addition to the library construction reagent container 22, the library construction system 20 can further be provided with a large-capacity reagent container 27 for storing large-capacity reagents such as magnetic beads, washing solution, and elution solution.

[0064] It should be noted here that the library construction manipulator component and the library construction pipetting component can be set independently. Considering that the library construction manipulator component and the library construction pipetting component do not need to work in parallel, they can also be integrated into one for space saving. In addition, the library construction system 20 can batch process the nucleic acid extraction products of multiple biological samples for library construction.

[0065] In an implementable manner, the library construction pipetting component can be a pipette (or a liquid transfer pump). To cooperate with the work of the pipette, a pipette tip for liquid transfer also needs to be equipped for the pipette. At the beginning of the liquid transfer work, the pipette sleevs the pipette tip on the pipette tip adapter at its end. After the liquid transfer work is completed, the pipette detaches the pipette tip from the pipette tip adapter at its end. Correspondingly, the library construction system 20 is also provided with a pipette tip storage area, and the used pipette tips will be put into the waste container 26, as Figure 4 shown.

[0066] For the electric control system 40, as Figure 5 shown, according to the functional division, the electric control system 40 mainly includes a central control module (or a central control board) 41, control modules that implement various control functions, and various sensors, which can collect information reflecting various operating states of the instrument.

[0067] Among them, the central control module 41, as the center connecting the host computer 50 and each control module, receives the control instructions sent by the host computer 50 through a communication interface (for example, an RS232 interface), and after parsing, verifying, and re-encoding, issues the instructions to the corresponding control module to achieve unified and orderly control of each control module. Each control module cooperates in an orderly manner under the control to achieve the purpose of constructing a sequencing library before gene sequencing.

[0068] As Figure 5As shown in the figure, the electronic control system 40 may include, for example, but is not limited to: a nucleic acid extraction system control module 42, a library construction system control module 43, a quality control system control module 44, and an auxiliary component control module 45. Among them, taking the purification by magnetic bead method as an example, the nucleic acid extraction system control module 42 at least includes: a temperature control module 420, a nucleic acid extraction platform movement control module 421, and a magnetic rod / magnetic rod sleeve movement control module 422; the quality control system control module 44 at least includes: a quality control manipulator assembly movement control module 440, a transfer mechanism movement control module 441, a quality control pipetting assembly movement control module 442, and a quantitative analyzer control module 443; the library construction system control module 43 at least includes: a library construction pipetting assembly movement control module 430, a library construction manipulator assembly movement control module 431, an oscillation heating assembly control module 432, and a refrigeration control module 433; the auxiliary component control module 45 at least includes: a camera control module 450, a puncturing component movement control module 451, an ultraviolet lamp and other light source control module 452, and an air cleaning control module 453. Each module will be described in detail below.

[0069] The temperature control module 420 can provide suitable reaction temperatures and reaction conditions for various chemical reactions (such as cell lysis reaction, washing reaction, elution reaction) occurring on the nucleic acid extraction platform by controlling heating devices. For example, the heating device here can be a heating sheet, or it can also be a TEC (thermoelectric cooler) module. And, in order to better achieve temperature control, the temperature control module 420 can also achieve closed-loop control according to the temperature collected by the temperature sensor.

[0070] The nucleic acid extraction platform movement control module 421 and the magnetic rod / magnetic rod sleeve movement control module 422 can cooperate with each other to realize the relative movement between the nucleic acid extraction platform and the magnetic rod / magnetic rod sleeve, and complete each operation step of nucleic acid extraction. Taking the movement of the nucleic acid extraction platform in a two-dimensional plane as an example, the nucleic acid extraction platform movement control module 421 can drive the X-direction motor and the Y-direction motor to move respectively by controlling the driver, and then drive the nucleic acid extraction platform to move in the X direction and the Y direction (the X direction and the Y direction are perpendicular to each other on the horizontal plane). The magnetic rod / magnetic rod sleeve movement control module 422 can drive the Z-direction motor to drive the magnetic rod and the magnetic rod sleeve to move in the Z direction respectively by controlling the driver (the Z direction is perpendicular to the horizontal plane formed by the X and Y directions).

[0071] The quality control manipulator assembly movement control module 440 can drive the X-direction motor, the Y-direction motor, and the Z-direction motor to move respectively by controlling the driver, and then drive the quality control manipulator assembly to move in the X direction, the Y direction, and the Z direction.

[0072] The transfer mechanism movement control module 441 can drive the X-direction motor to drive the transfer mechanism to move in the X direction by controlling the driver.

[0073] The quality control pipetting assembly motion control module 442 can drive the X-axis motor, Y-axis motor, and Z-axis motor to move respectively by controlling the driver, thereby driving the quality control pipetting assembly to move in the X-axis, Y-axis, and Z-axis directions.

[0074] The quantitative analyzer control module 443 can control the quantitative analyzer to detect the concentration of at least one of the nucleic acid extraction product and the library construction product, and output the detection result.

[0075] The library construction pipetting assembly motion control module 430 can drive the X-axis motor, Y-axis motor, and Z-axis motor to move respectively by controlling the driver, thereby driving the library construction pipetting assembly to move in the X-axis, Y-axis, and Z-axis directions.

[0076] The library construction manipulator assembly motion control module 431 can drive the X-axis motor, Y-axis motor, and Z-axis motor to move respectively by controlling the driver, thereby driving the library construction manipulator assembly to move in the X-axis, Y-axis, and Z-axis directions.

[0077] The oscillation heating assembly control module 432 can provide suitable reaction temperatures and reaction conditions for various reactions in magnetic bead purification (such as washing reaction, elution reaction) by controlling the oscillation device and heating device. For example, the heating device here can be a heating sheet, or it can also be a TEC (thermoelectric cooler) module. And, in order to better achieve temperature control, the oscillation heating assembly control module 432 can also achieve closed-loop control according to the temperature collected by the temperature sensor.

[0078] The reagent container refrigeration control module 433 can provide a suitable refrigeration temperature for the library construction reagent container 22 by controlling the heating device and refrigeration device. For example, the heating device here can be a TEC (thermoelectric cooler) module, and the refrigeration device can be a fan. And, in order to better achieve temperature control, the refrigeration control module 433 can also achieve closed-loop control according to the temperature collected by the temperature sensor.

[0079] The reagent addition area refrigeration control module 434 can provide a suitable refrigeration temperature for the reagent addition area by controlling the heating device and refrigeration device. For example, the heating device here can be a TEC (thermoelectric cooler) module, and the refrigeration device can be a fan. And, in order to better achieve temperature control, the reagent addition area refrigeration control module 434 can also achieve closed-loop control according to the temperature collected by the temperature sensor.

[0080] The camera control module 440 can drive the X-axis motor, Y-axis motor, and Z-axis motor to move respectively by controlling the driver, thereby driving the camera in the X-axis, Y-axis, and Z-axis directions, and controlling the camera to take pictures of the consumables (such as extraction operation containers, library construction reagent containers) in the sequencing pre-treatment instrument after reaching the target position, and report them to the host computer. For example, a two-dimensional code is set on the consumable, and the two-dimensional code carries consumable information. By controlling the camera to take pictures of the two-dimensional code on the consumable, the host computer can identify the consumable information according to the two-dimensional code. In addition, the camera control module can also control the camera to take pictures of relevant containers (such as the library construction reagent container 22, the construction reaction container 35, the purification container placed on the oscillation heating component 25, the pipette tip container placed in the pipette tip storage area for accommodating pipette tips used for pipetting by the pipette, etc.) and report them to the host computer 50, so that the host computer 50 can verify the placement position, placement direction of the relevant containers and the relevant consumable information before the pre-treatment experiment starts, and determine whether each container is placed correctly and whether the consumables are used correctly.

[0081] The piercing component movement control module 441 can drive the X-axis motor, Y-axis motor, and Z-axis motor to move respectively by controlling the driver, thereby driving the piercing component to move in the X-axis, Y-axis, and Z-axis directions.

[0082] The ultraviolet lamp and other light source control module 442 includes: an ultraviolet lamp control sub-module, a status indicator light control sub-module, and a decorative lamp control sub-module ( Figure 4 not shown in the figure). Among them, the ultraviolet lamp control sub-module can control the ultraviolet lamp to work in a stable light power state, so that the ultraviolet rays emitted by the ultraviolet lamp can disinfect the sequencing pre-treatment instrument; the status indicator light control sub-module can control the indicator light to work in a stable light power state and indicate the working state of the sequencing pre-treatment instrument by controlling the color of the indicator light; the decorative lamp control sub-module can control the light strip to work in a stable light power state to increase the aesthetics of the sequencing pre-treatment instrument.

[0083] The air cleaning control module 443 provides clean air for the inside of the instrument chassis and the inside of the nucleic acid extraction system by controlling the air inlet mechanism (such as a blower) and the air exhaust mechanism (such as a fan).

[0084] In addition to the above various control modules, the electric control system 40 also needs to collect information reflecting the operation state of the instrument in real time through various sensors and report it to the host computer 50 through the central control module 41, which is used for the host computer 50 to detect the working state of the instrument and ensure the stable operation of the instrument.

[0085] Various sensors may include, for example, but are not limited to: temperature sensors for collecting the temperatures of key components inside the sequencer, sensors for detecting the in-place status of relevant components (including but not limited to limit position detection and zero position detection), etc.

[0086] Among them, the in-place status detection may include, for example, but is not limited to: the in-place status detection of the nucleic acid extraction platform, the magnetic bar / magnetic bar sleeve assembly, the quality control manipulator assembly, the conveying mechanism, the quality control pipetting assembly, the library construction manipulator assembly, the library construction pipetting assembly, the nucleic acid extraction system chamber door, and the instrument chamber door.

[0087] For example, the temperature sensor may collect the temperatures of the nucleic acid extraction platform, the oscillating heating assembly, the reagent addition area, etc., and the central control module may further form a closed-loop control based on the temperatures collected by the temperature sensor.

[0088] In addition, for example, the temperature sensor may also collect the temperature of the heat sink in the TEC module, and the central control module may further determine whether the TEC module is operating normally based on the temperature of the heat sink, and perform controls such as alarming and stopping operation in case of abnormal operation.

[0089] After the central control module 41 reports the information collected by the sensor to the host computer 50, the host computer 50 detects the working status of the instrument, and issues an instruction indicating the working status according to the detection result. The central control module 41 forwards the instruction to the status indicator light control sub-module, and the status indicator light control sub-module controls the indicator light to indicate the working status of the instrument according to the instruction. For example, the RGB three-color indicator light is used to indicate the working status of the instrument (for example, including the running status, the fault status, etc.).

[0090] The central control module 41 can also receive the input power supply, convert the input power supply into the working power supplies required by each control module according to the needs, and output them.

[0091] The above are the nucleic acid extraction and library construction and other process operations and related systems involved in the embodiments of the present application. In specific implementation, the above systems can be uniformly set in the gene sequencing pre-processing device 1 (such as Figure 6As shown in [figure], the nucleic acid extraction and library construction processes are both completed in the pre-gene sequencing processing device 1. After each nucleic acid extraction and library construction process, nucleic acid fragments caused by residual aerosol or splashing will remain on the inner wall of the instrument. If these nucleic acid fragments are not processed, it is very likely that they will contaminate new batches of samples during the next experiment, thus affecting the quality of library construction. Therefore, it is necessary to disinfect the inside of the instrument after each nucleic acid extraction and library construction process. In order to complete the nucleic acid disinfection work inside the pre-gene sequencing processing device 1 after each nucleic acid extraction and library construction process, keep the experimental environment clean, and ensure the accuracy of experimental results, the present application provides a fully automatic disinfection kit, which can automatically perform nucleic acid disinfection work inside the instrument and can effectively solve the problem of difficult treatment of dead corners, liberating the labor force.

[0092] As Figures 8 to 10 shown, on the one hand, the fully automatic disinfection kit 2 provided by the present application includes: an atomizing head 2-1, a kit body 2-2, and a kit cover 2-3. The atomizing head 2-1 is in fluid communication with the kit body 2-2. The kit body 2-2 is used to accommodate the disinfection reagent. The kit cover 2-3 is arranged at the upper end of the kit body 2-2. A power connection part 2-4 and a docking part 2-7 are arranged on the upper side of the kit cover 2-3. The power connection part 2-4 is electrically connected to the atomizing head 2-1. The docking part 2-7 is used to dock with the driving component to drive the movement of the fully automatic disinfection kit 2. A fogging outlet communicating with the inside of the kit body 2-2 is also arranged on the kit body 2-2 and / or the kit cover 2-3.

[0093] Specifically, the fully automatic disinfection kit provided by the present application is provided with an atomizing head 2-1 inside the kit body 2-2. The atomizing head 2-1 is in fluid communication with the kit body 2-2, and can atomize the nucleic acid disinfectant inside the kit body 2-2 and disperse it through the fogging outlet on the kit body 2-2 and / or the kit cover 2-3. The kit cover 2-3 is installed above the kit body 2-2, and the two are connected by heat melting. A power connection part for connecting the atomizing head 2-1 and a docking part are arranged at the upper end of the kit cover 2-3, which can provide power for the atomizing head 2-1. The docking part is docked with the driving component to drive the movement of the fully automatic disinfection kit 2. In the pre-gene sequencing processing device 1, the driving component can be the three-axis robotic arm 1-2 and the pipette 3 already equipped in the pre-gene sequencing processing device 1 for pipetting reagents. Connecting this fully automatic disinfection kit through the three-axis robotic arm 1-2 and the pipette 3 can move it to disperse the nucleic acid disinfectant to all corners of the pre-gene sequencing processing device 1, thereby achieving comprehensive disinfection of the internal space of the pre-gene sequencing processing device 1, and no additional driving component needs to be added, saving costs.

[0094] Optionally, a partition 2-9 is provided inside the bottom of the kit body 2-2. The partition 2-9 is provided with a through hole, and the atomizing head 2-1 passes through the through hole and is arranged on the partition 2-9 and accommodated inside the kit body.

[0095] Specifically, the partition 2-9 is located slightly below the middle of the kit body 2-2, so that a downward-opening installation groove for accommodating the atomizing head 2-1 is formed below the kit body. The atomizing head 2-1 is arranged in the installation groove and installed through the through hole on the partition 2-9. A disinfection kit board position 1-1 is provided in the gene sequencing pretreatment device 1 to place the disinfection kit 2. An installation groove is provided below the kit body 2-2 of the disinfection kit 2. On the one hand, it provides an installation space for the atomizing head and also facilitates placing the disinfection kit 2 on the disinfection kit board position 1-1.

[0096] Optionally, the atomizing head is hermetically connected to the partition 2-9.

[0097] Specifically, the atomizing head 2-1 adopts an ultrasonic atomizing head 2-1. A through hole is provided on the partition 2-9. The upper flange of the ultrasonic atomizing head 2-1 is installed in the through hole. The upper end face of the ultrasonic atomizing head 2-1 is attached to the bottom surface of the partition 2-9, and the two are fixed by screws 2-6 to form a sealed connection to prevent liquid leakage.

[0098] Optionally, the docking part 2-7 is a docking hole or a docking post, and the docking part 2-7 can be arranged in the middle of the kit cover 2-3.

[0099] Specifically, as Figure 8 、 Figure 9 shown, the docking part 2-7 in the middle of the kit cover 2-3 is set as a docking hole, which is convenient for connecting with the pipette tip adapter 3-2 on the pipette 3 (as Figure 11 shown). The pipette tip adapter 3-2 is set as a docking post. The docking post of the pipette tip adapter 3-2 is inserted into the docking hole, so as to connect the fully automatic disinfection kit 2 to the pipette 3 and move the disinfection kit 2 through the pipette 3. Optionally, in other embodiments, the docking part 2-7 of the kit cover 2-3 is set as a docking post on the upper part and a docking hole on the pipette 3 to form a matching connection structure. In this way, the existing pipette in the gene sequencing pretreatment device 1 can be used, and in the form of a docking hole or a docking post, it is convenient for quick plugging and unplugging of the pipette adapter of the pipette 3, and the use is simple and convenient.

[0100] Optionally, the power connection part 2-4 is a power male contact or a power female contact.

[0101] Specifically, as Figure 8 、 Figure 11 shown, the power connection part 2-4 is arranged above the kit cover 2-3 and is electrically connected to the ultrasonic atomizing head 2-1. In one embodiment, the power connection part on the kit cover 2-3Figure 8 Shown is a male power contact, which can be connected to the female power contact 3-1 of the pipette 3 to supply power to the ultrasonic atomizing head 2-1. Optionally, the power connection part 2-4 on the kit cover 2-3 is set as a female power contact, and the pipette 3 is set as a male power contact.

[0102] Optionally, a liquid injection hole 2-8 is provided on the kit cover 2-3, and a plug cover 2-5 is provided corresponding to the liquid injection hole 2-8.

[0103] Specifically, as Figure 8 、 Figure 9 shown, a liquid injection hole 2-8 is provided on the kit cover 2-3 for manually adding reagents into the kit, and the plug cover 2-5 is installed in the liquid injection hole 2-8 of the kit cover 2-3 for sealing after adding reagents.

[0104] Optionally, the outlet direction of the atomization outlet is set downward.

[0105] Specifically, as Figure 10 shown, the atomization outlet is provided on the kit cover 2-3 to communicate the inside and outside of the kit body 2-2, so that the atomized nucleic acid disinfectant in the kit body 2-2 can be dispersed.

[0106] On the other hand, the present application provides a gene sequencing pretreatment device 1, including a driving component and a full-automatic disinfection kit 2 as described above. The driving component includes a driving assembly and a pipette 3. The pipette 3 is arranged on the driven assembly, and the end of the pipette 3 is adapted to the power connection part and the docking part of the full-automatic disinfection kit 2.

[0107] Specifically, as Figure 6 、 Figure 7 shown, several plate positions are provided in the gene sequencing pretreatment device 1, including a disinfection kit plate position 1-1. The full-automatic disinfection kit 2 can be placed at the disinfection kit plate position 1-1 in a non-working state. The end of the pipette 3 is adaptively connected to the power connection part and the docking part of the full-automatic disinfection kit 2. The pipette 3 is installed on the driving assembly. The driving assembly can be specifically set as a three-axis robotic arm 1-2. The three-axis robotic arm 1-2 drives the pipette 3 to move flexibly along the X, Y, and Z directions, so as to drive the full-automatic disinfection kit 2 to move along the X, Y, and Z directions in the gene sequencing pretreatment device 1 to disperse the nucleic acid disinfectant.

[0108] Optionally, a pipette tip adapter 3-2 is provided at the end of the pipette 3. The pipette tip adapter 3-2 is a docking column, and the docking column is inserted into the docking part 2-7 of the full-automatic disinfection kit to fix the full-automatic disinfection kit to the driving component.

[0109] Specifically, as Figure 9 、Figure 11 , Figure 12 As shown, the docking part 2-7 in the middle of the kit cover 2-3 is set as a docking hole, which is convenient for connecting with the pipette tip adapter 3-2 on the pipette 3 (such as Figure 11 shown). The pipette tip adapter 3-2 is set as a docking post, and the docking post of the pipette tip adapter 3-2 is inserted into the docking hole, thereby connecting the fully automatic disinfection and sterilization kit 2 to the pipette 3, and moving the disinfection and sterilization kit 2 through the pipette 3. Optionally, the docking part can be set as a docking post on the kit cover 2-3 and a docking hole on the pipette 3 to form a matching connection structure. In this way, the existing pipette in the gene sequencing pretreatment device 1 can be used, and in the form of a docking hole or a docking post, it is convenient for quick plugging and unplugging with the pipette adapter of the pipette 3, and the use is simple and convenient.

[0110] Optionally, a power supply female contact 3-1 is provided at the end of the pipette 3, and the power supply connection part of the fully automatic disinfection and sterilization kit is a power supply male contact, and the power supply female contact 3-1 is electrically connected to the power supply male contact to supply power to the atomizing head of the fully automatic disinfection and sterilization kit.

[0111] Specifically, as Figure 8 , Figure 11 , Figure 12 shown, the power supply connection part 2-4 is a power supply male contact, which can be cooperatively connected with the power supply female contact on the pipette 3 to supply power to the ultrasonic atomizing head 2-1.

[0112] Optionally, the driving component is a library construction pipetting assembly or a quality control pipetting assembly.

[0113] Specifically, using the library construction pipetting assembly or the quality control pipetting assembly as the driving component, that is, using the original structure of the device to drive the fully automatic disinfection and sterilization kit, the fully automatic disinfection and sterilization kit can be integrated into the original gene sequencing pretreatment device with the least modification, and the fully automatic and comprehensive disinfection and sterilization function can be realized.

[0114] The following provides a specific implementation manner of the present application:

[0115] As Figure 6 shown, when in use, the fully automatic nucleic acid disinfection and sterilization kit is placed on the disinfection and sterilization kit board position 1-1 of the gene sequencing pretreatment device 1. The upper computer program controls the three-axis robotic arm to drive the pipette 3 to move above the disinfection and sterilization kit board position 1-1, and then move downward along the Z-axis to insert the pipette tip adapter on the pipette 3 into the docking part 2-7 of the fully automatic nucleic acid disinfection and sterilization kit, and connect the pipette 3 and the fully automatic nucleic acid disinfection and sterilization kit 2 tightly through interference fit; at this time, as Figure 12 shown, the power supply connection part 2-4 of the fully automatic nucleic acid disinfection and sterilization kit 2 is connected to the power supply female contact 3-1 on the pipette 3, so that the ultrasonic atomizing head 2-1 is powered on and starts to work; then as Figure 7As shown in the figure, the upper computer program controls the three-axis robotic arm 1-2 to drive the pipette 3 to slowly move along the set route inside the gene sequencing pretreatment device 1, so as to evenly distribute the atomized nucleic acid disinfection reagent on the tabletop and inner wall of the gene sequencing pretreatment device 1, thus realizing the function of fully automatic disinfection.

[0116] This application can realize the function of fully automatic nucleic acid fragment disinfection. Moreover, the atomized reagent can easily enter into each dead corner, achieving more thorough disinfection, without the need for manual wiping, thus saving labor costs.

[0117] In the description of this application, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing this application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, so it cannot be understood as a limitation to this application. In addition, the terms "first", "second", "third" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0118] In the description of this application, it should be noted that unless otherwise clearly specified and limited, the terms "installed", "connected", "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, or an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in this application can be understood according to specific situations.

[0119] The above is only the specific implementation manner of this application, but the protection scope of this application is not limited thereto. Any person skilled in the art within the technical scope disclosed by this application can easily think of changes or substitutions, which should all be covered within the protection scope of this application. Therefore, the protection scope of this application should be subject to the protection scope of the claims.

Claims

1. A fully automatic disinfection kit, characterized in that: include: An atomizer head, a reagent box body and a reagent box cover, wherein the atomizer head is fluidically connected to the reagent box body, the reagent box cover is arranged at the upper end of the reagent box body, a power connection part and a docking part are arranged on the upper side of the reagent box cover, the power connection part is electrically connected to the atomizer head, and the docking part is used to dock with a driving component to drive the full-automatic disinfection reagent box to move, and an atomization outlet connected to the inside of the reagent box body is also arranged on the reagent box body and / or the reagent box cover.

2. The fully automatic disinfection kit according to claim 1, characterized in that: A partition is arranged inside the reagent box body, a through hole is arranged on the partition, and the atomizing head passes through the through hole and is arranged on the partition and accommodated inside the reagent box body.

3. The fully automatic disinfection kit according to claim 2, characterized in that: The atomizing head is sealed and connected to the partition.

4. The fully automatic disinfection kit according to claim 1, characterized in that: The docking portion is a docking hole or a docking column.

5. The fully automatic disinfection kit according to claim 1, characterized in that: The power connection portion is a power male contact or a power female contact.

6. The fully automatic disinfection kit according to claim 1, characterized in that: The reagent kit cover is provided with a liquid injection hole, and a plug cover is provided corresponding to the liquid injection hole.

7. The fully automatic disinfection kit according to claim 1, characterized in that: The outlet direction of the atomization outlet is arranged downward.

8. A gene sequencing pre-processing device, characterized in that: It comprises a driving component and a fully automatic disinfection kit as described in any one of claims 1-7, wherein the driving component comprises a driving assembly and a pipette, the pipette is arranged on the driving assembly, and the end of the pipette is adapted to the power connection part and the docking part of the fully automatic disinfection kit.

9. The gene sequencing pre-processing device according to claim 8, characterized in that: A pipette head adapter is provided at the end of the pipette, and the pipette head adapter is a docking column, and the docking column is inserted into the docking part of the full-automatic disinfection reagent kit to fix the full-automatic disinfection reagent kit to the driving component.

10. The gene sequencing pre-processing device according to claim 8, characterized in that: A female power contact is provided at the end of the pipette, and the power connection part of the full-automatic disinfection kit is a male power contact. The female power contact is electrically connected to the male power contact to power the atomization head of the full-automatic disinfection kit.

11. The gene sequencing pre-processing device according to claim 8, characterized in that: The gene sequencing pre-processing equipment also includes a reagent kit accommodating seat, and the fully automatic disinfection reagent kit can be placed on the reagent kit accommodating seat in a non-working state.

12. The gene sequencing pre-processing device according to any one of claims 8 to 10, characterized in that: The driving component is a library construction pipetting component or a quality control pipetting component.

13. The gene sequencing pre-processing device according to any one of claims 8 to 10, characterized in that: The driving assembly is a three-axis robotic arm.

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