Biological enzyme flavor absorbing towel based on bacillus subtilis and preparation method of biological enzyme flavor absorbing towel

Abstract

The invention relates to the technical field of odor-absorbing towels, in particular to a bacillus subtilis-based biological enzyme odor-absorbing towel and a preparation method thereof.The bacillus subtilis-based biological enzyme odor-absorbing towel comprises the following four layers: a surface layer, a breathable anti-reverse-osmosis non-woven fabric or nano-silver coating material, the pore diameter of the surface layer is smaller than or equal to 0.1 micron, and the surface layer is used for gas adsorption and liquid blocking; the biological enzyme filter membrane layer is formed by loading bacillus subtilis spores (greater than or equal to 108 CFU / g) and immobilized enzymes (lipase and protease) by a sodium alginate / chitosan composite hydrogel membrane; the specific surface area of the adsorption layer is larger than or equal to 1200 m < 2 > / g, the H2S adsorption capacity of the adsorption layer is larger than or equal to 200 mg / g, and the adsorption layer is used for pre-adsorbing high-concentration sulfides; the bottom layer is a microporous PE leakage-proof film, and the humidity is maintained at 60%-80% RH. According to the invention, peculiar smell molecules can be effectively degraded, liquid reverse osmosis can be prevented, and good air permeability and humidity regulation and control capability can be realized.

Description

Technical Field

[0001] The present invention relates to the technical field of odor-absorbing towels, and particularly to a biological enzyme odor-absorbing towel based on Bacillus subtilis and a preparation method thereof, which is particularly suitable for treating the odor of human anal parts or pet excrement, and can be integrated into underwear liners, including disposable underwear, sanitary napkins or pet urine pads. Background Art

[0002] The current sanitary napkin is a narrow concept, mainly referring to the function of absorbing women's menstrual blood. Some have the function of removing odors, but mainly to eliminate the odor generated by menstrual blood. For example, antibacterial agents such as tea polyphenols and paclitaxel silver ions are used to reduce the generation of odors, and activated carbon, Phoebe zhennan wood particles, coffee grounds, hydrophobic zeolite powder, and adsorption methods are used to remove odors. Cedar alcohol crystal particles, linalool essential oil sustained-release tablets, and aromatic Chinese herbal medicines are used to cover the odor with fragrance. These methods have problems such as low removal efficiency and easy secondary pollution. Therefore, the existing sanitary napkin technology cannot be used as a generalized sanitary napkin to simultaneously remove the smell of farts. For the high-concentration sulfide (such as H2S), ammonia and other odor substances generated by human and pet excrement, there is an urgent need to develop an efficient, environmentally friendly and safe solution.

[0003] Therefore, those skilled in the art have provided a biological enzyme odor-absorbing towel based on Bacillus subtilis and a preparation method thereof to solve the problems raised in the above background art. Summary of the Invention

[0004] To solve the above technical problems, the present invention provides a biological enzyme odor-absorbing towel based on Bacillus subtilis,

[0005] including the following four layers:

[0006] The surface layer, a breathable and anti-reverse osmosis non-woven fabric or a nano-silver coating material with a pore diameter ≤ 0.1 μm, for gas adsorption and liquid barrier;

[0007] The biological enzyme filter membrane layer, composed of a sodium alginate / chitosan composite hydrogel membrane loaded with Bacillus subtilis spores (≥ 10^8 CFU / g) and immobilized enzymes (lipase, protease);

[0008] The adsorption layer, activated carbon fiber or MOFs material, with a specific surface area ≥ 1200m 2 / g and an H2S adsorption capacity ≥ 200 mg / g, for pre-adsorbing high-concentration sulfide;

[0009] The bottom layer, a microporous PE leak-proof membrane, maintaining the humidity at 60%-80% RH.

[0010] Preferably: the survival rate of the Bacillus subtilis spores ≥ 90% (stored dry at 25°C for 30 days), and the metabolites include subtilin, polymyxin and sulfide oxidase.

[0011] Preferably, a sustained-release nutrient matrix is added to the sodium alginate / chitosan hydrogel film, which contains 5%-8% by mass of glucose and 3%-5% by mass of peptone, and forms a porous network structure through hydrogen bond cross-linking.

[0012] Preferably, the MOFs material is a ZIF-8 type metal-organic framework material, with a specific surface area ≥ 1200 m 2 / g and an H2S adsorption capacity ≥ 200 mg / g.

[0013] Preferably, the pore size of the microporous PE leak-proof film is 10-50 μm, and the microporous PE leak-proof film forms a gradient wettability through surface plasma treatment.

[0014] Preferably, the surface layer is a nano-silver coating material, and the silver ion loading in the nano-silver coating material is 0.1-0.5 mg / cm 2 ; the bio-enzyme filter membrane layer is sterilized by ethylene oxide treatment, and the residue amount ≤ 1 μg / g.

[0015] Preferably, under the conditions of 30 °C and 80% humidity, the 8-hour removal rate of 50 ppm H2S ≥ 85%, the air permeability ≥ 500 mL / (cm 2 ·s), and the survival rate of the bacteria decreases by ≤ 10% after 50 foldings.

[0016] The present invention also provides a preparation method of a bio-enzyme odor-absorbing towel based on Bacillus subtilis, including the steps:

[0017] Expansion culture and freeze-drying of Bacillus subtilis spores, with a spore density ≥ 10^10 CFU / mL;

[0018] Mixing the spores, immobilized enzymes and nutrient matrix with the sodium alginate / chitosan solution, and casting into a film;

[0019] Drying the film layer at low temperature under vacuum (-40 °C, 0.1 Pa), with a moisture content ≤ 5%;

[0020] Thermocompression lamination with the adsorption layer, surface layer and bottom layer (temperature 80-100 °C, pressure 0.5-1.0 MPa);

[0021] Irradiation sterilization (dose 15-25 kGy).

[0022] Preferably, 2%-5% by mass of trehalose or mannitol is added as a freeze-drying protectant during the freeze-drying process of the spores.

[0023] The technical effects and advantages of the present invention:

[0024] The present invention designs an odor-absorbing towel with a multi-layer composite structure by combining genetically engineered Bacillus subtilis with biological enzymes, which can effectively degrade odor molecules, prevent liquid back-seepage, and at the same time has good air permeability and humidity regulation ability.

[0025] Filling the gap, meeting the needs of the modern civilized era, reducing the sudden attacks of emotions of oneself and others when there is a lot of flatulence, interrupting thinking, eliminating a lot of embarrassments, and having broad application prospects.

[0026] The odor-absorbing towel of the present invention is applicable to the treatment of body odor in the anal area of the human body or pet excrement, and can be integrated into underwear liners, sanitary napkins or pet urine pads, and is widely used in the fields of family, medical care and pet care. Description of the Drawings

[0027] Figure 1 It is a process flow chart of the preparation of the odor-absorbing towel provided by the embodiment of the present application. Detailed Description of the Invention

[0028] The present invention will be further described in detail below in conjunction with the drawings and specific embodiments. The embodiments of the present invention are given for purposes of illustration and description, and are not exhaustive or limiting the present invention to the disclosed form. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiments are selected and described in order to better illustrate the principles and practical applications of the present invention, and to enable those of ordinary skill in the art to understand the present invention and thus design various embodiments with various modifications suitable for specific purposes.

[0029] Example 1

[0030] Please refer to Figure 1 , in this embodiment, a biological enzyme odor-absorbing towel based on Bacillus subtilis is provided, which includes the following four layers: the surface layer, made of breathable and anti-back-seepage non-woven fabric or nano-silver coating material, with a pore size ≤ 0.1 μm, for gas adsorption and liquid barrier; the biological enzyme filter membrane layer, composed of sodium alginate / chitosan composite hydrogel membrane loaded with Bacillus subtilis spores (≥ 10^8 CFU / g) and immobilized enzymes (lipase, protease), to achieve efficient degradation of odor molecules; the adsorption layer, using activated carbon fibers or MOFs materials, with a specific surface area ≥ 1200 m 2 / g, with an H2S adsorption capacity ≥ 200 mg / g, pre-adsorbing high-concentration sulfides; the bottom layer, a microporous PE leak-proof membrane, with a pore size of 10 - 50 μm, forming a gradient wettability through surface plasma treatment, maintaining the humidity at 60% - 80% RH and preventing the leakage of bacteria.

[0031] Among them, the survival rate of Bacillus subtilis spores ≥ 90% (stored dry at 25°C for 30 days), and the metabolites include subtilin, polymyxin, and sulfide oxidase, which further enhance the degradation effect on odor molecules. The Bacillus subtilis is a genetically engineered strain and does not carry antibiotic resistance genes.

[0032] Among them, a slow-release nutrient matrix is added to the sodium alginate / chitosan hydrogel film, which contains 5%-8% glucose and 3%-5% peptone by mass fraction, and forms a porous network structure through hydrogen bond cross-linking, prolonging the active life of the bacteria and improving the degradation efficiency.

[0033] Among them, the MOFs material is selected as the ZIF-8 type metal-organic framework material, with a specific surface area ≥ 1200 m 2 / g and an adsorption capacity for H2S ≥ 200 mg / g, significantly improving the adsorption ability for high-concentration sulfides.

[0034] Among them, the bottom microporous PE leak-proof membrane forms a gradient wettability through surface plasma treatment, realizing humidity-responsive spore activation and ensuring the activity of the bacteria in a suitable environment.

[0035] Among them, the surface layer is a nano-silver coating material, and the silver ion loading in the nano-silver coating material is 0.1-0.5 mg / cm 2 ; the bio-enzyme filter membrane layer is sterilized by ethylene oxide treatment, and the residue ≤ 1 μg / g.

[0036] The test results show that at 30°C and 80% humidity, the 8-hour removal rate of 50 ppm H2S ≥ 85%, the air permeability ≥ 500 mL / (cm 2 ·s), and the survival rate of the bacteria decreases ≤ 10% after 50 bends, showing excellent performance and durability.

[0037] Example 2

[0038] This example provides a preparation method of an odor-absorbing towel, and the specific steps are as follows: Bacillus subtilis spore expansion and freeze-drying, with a spore density ≥ 10^10 CFU / mL; mixing the spores, immobilized enzyme, and nutrient matrix with the sodium alginate / chitosan solution and casting into a film; drying the film layer at low temperature under vacuum (-40°C, 0.1 Pa), with a moisture content ≤ 5%; thermally pressing and laminating the bio-enzyme filter membrane layer with the adsorption layer, surface layer, and bottom layer (temperature 80-100°C, pressure 0.5-1.0 MPa); irradiating and sterilizing (dose 15-25 kGy).

[0039] Among them, during the spore freeze-drying process, 2%-5% trehalose or mannitol by mass fraction is added as a freeze-drying protectant.

[0040] Among them, the silver ion loading in the surface nano-silver coating is 0.1-0.5 mg / cm2 ; The bio-enzyme filter membrane layer is sterilized by ethylene oxide, and the residue amount is ≤ 1 μg / g.

[0041] Industrial feasibility: The casting film forming and hot pressing composite process is adopted to adapt to the existing hygiene product production line.

[0042] Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments. All other embodiments obtained by those of ordinary skill in the art and related fields based on the embodiments in the present invention without creative efforts shall fall within the protection scope of the present invention. The structures, devices, and operation methods not specifically described and explained in the present invention shall be implemented by conventional means in the art without special instructions and limitations.

Claims

1. A bio-enzyme odor-absorbing towel based on Bacillus subtilis, characterized in that: It includes the following four layers: The surface layer is a breathable and anti-reverse osmosis non-woven fabric or nano-silver coating material with a pore size of ≤0.1μm, which is used for gas adsorption and liquid barrier; The biological enzyme filter membrane layer is composed of a sodium alginate / chitosan composite hydrogel membrane loaded with Bacillus subtilis spores (≥10^8 CFU / g) and immobilized enzymes (lipase, protease); Adsorption layer, activated carbon fiber or MOFs material, specific surface area ≥ 1200m 2 / g, H2S adsorption capacity ≥ 200mg / g, used for pre-adsorption of high concentration sulfide; The bottom layer, microporous PE leakproof film, maintains humidity at 60%-80% RH.

2. The bio-enzyme odor-absorbing towel based on Bacillus subtilis according to claim 1, characterized in that: The survival rate of the Bacillus subtilis spores is ≥90% (dried and stored at 25°C for 30 days), and the metabolites include subtilisin, polymyxin and sulfide oxidase.

3. The bio-enzyme odor-absorbing towel based on Bacillus subtilis according to claim 1, characterized in that: A slow-release nutrient matrix is ​​added to the sodium alginate / chitosan hydrogel film, comprising 5%-8% by mass of glucose and 3%-5% by mass of peptone, and a porous network structure is formed by hydrogen bond cross-linking.

4. The bio-enzyme odor-absorbing towel based on Bacillus subtilis according to claim 1, characterized in that: The MOFs material is a ZIF-8 type metal organic framework material with a specific surface area of ​​≥1200m 2 / g, H2S adsorption capacity ≥200mg / g.

5. The bio-enzyme odor-absorbing towel based on Bacillus subtilis according to claim 1, characterized in that: The pore size of the microporous PE leakproof membrane is 10-50 μm, and the microporous PE leakproof membrane is subjected to surface plasma treatment to form gradient wettability.

6. The bio-enzyme odor-absorbing towel based on Bacillus subtilis according to claim 1, characterized in that: The surface layer is a nano silver coating material, and the silver ion loading in the nano silver coating material is 0.1-0.5 mg / cm 2 ; The biological enzyme filter membrane layer is sterilized by ethylene oxide, and the residual amount is ≤1μg / g.

7. The bio-enzyme odor-absorbing towel based on Bacillus subtilis according to claim 1, characterized in that: At 30℃ and 80% humidity, the removal rate of 50ppm H2S in 8 hours is ≥85%, and the air permeability is ≥500mL / (cm 2 ·s), after 50 bends, the survival rate of the bacteria decreased by ≤10%.

8. The bio-enzyme odor-absorbing towel based on Bacillus subtilis according to any one of claims 1 to 7, characterized in that: The odor-absorbing towel is suitable for treating odors in the human anus or pet excrement, and can be integrated into underwear liners, sanitary napkins or pet urine pads.

9. The method for preparing the bio-enzyme odor-absorbing towel based on Bacillus subtilis according to any one of claims 1 to 8, characterized in that: Includes steps: Bacillus subtilis spore expansion and freeze-drying, spore density ≥ 10^10 CFU / mL; The spores, immobilized enzymes and nutrient matrix are mixed with sodium alginate / chitosan solution and cast into a film; The film layer is vacuum dried at low temperature (-40℃, 0.1Pa), and the moisture content is ≤5%; Hot pressing compound with adsorption layer, surface layer and bottom layer (temperature 80-100℃, pressure 0.5-1.0MPa); Sterilize by irradiation (dose 15-25 kGy).

10. The method for preparing the bio-enzyme odor-absorbing towel based on Bacillus subtilis according to claim 9, characterized in that: During the freeze-drying process of the spores, 2%-5% of trehalose or mannitol is added as a freeze-drying protective agent.

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