Trace biological material evidence collecting and storing set

Abstract

The invention relates to the technical field of biological sample extraction, and discloses a trace biological material evidence collecting and storing suit which comprises an outer sleeve, a sleeve assembly is arranged on the outer sleeve, and the sleeve assembly is used for collecting and storing cotton fibers attached with biological samples. The sleeve assembly comprises an inner filter tube, and an outer sleeve comprises a barb, a spring clamping piece, a first groove, an oblique conical surface, a sealing ring, a cotton swab rod, a fiber fixing sleeve and cotton fibers. According to the trace biological material evidence collecting and storing set, the cotton swab rod is just fixed between the two positioning grooves, then the cover is uncovered and covers the outer sleeve, meanwhile, the cotton swab rod can be pulled out backwards, and the barb can be just inserted into the gap of the fiber fixing sleeve, so that cotton fibers are completely collected into the inner filter pipe; the biological material evidence can be completely stored, and the treatment efficiency of the biological material evidence collecting cotton swabs can be improved.

Description

Technical Field

[0001] The present invention relates to the technical field of biological sample extraction, and specifically to a set for collecting and preserving trace biological evidence. Background Art

[0002] With the development of DNA technology, it has become a key technology for public security organs to solve various cases by extracting and testing biological samples left by suspects at the scene and then determining the identity of the perpetrator through DNA identification. According to Locard's theory of material transfer, as long as there is contact on the surface of an object, there will be a transfer of trace substances on the surface of these objects. Therefore, biological samples of the perpetrator will be left at every crime scene. Investigators need to collect and preserve these trace biological samples for DNA testing and identification in a DNA laboratory, and this collection and preservation process needs to be achieved with the help of a special set for extracting trace biological evidence.

[0003] Trace biological evidence has the characteristics of low content, difficult extraction, and easy loss, becoming a "stumbling block" hindering case detection.

[0004] First, the cotton swab head of the existing biological evidence collection cotton swab is firmly wound with cotton fibers on the swab stick body. When laboratory testing personnel cut the cotton fibers attached with trace biological samples on the cotton swab head, the fibers are not easily separated from the cotton swab completely, and the cutting and tearing process will cause the loss of trace biological samples.

[0005] Second, after the existing biological evidence collection cotton swab wipes the biological sample, the collected biological sample is loosely attached to the surface of the cotton swab head. During the process of moving, transporting, and handing over after the cotton swab is put into the evidence packaging bag, the trace biological samples loosely attached to the surface of the cotton swab head will fall off.

[0006] Third, after the biological evidence extraction cotton swab collects the biological sample and is put into the evidence packaging bag, due to the introduction of bacteria and other microorganisms in the environment during the wiping process, it is easy to cause the biological sample to be decomposed by microorganisms, resulting in a decrease in DNA content.

[0007] Fourth, in the DNA extraction step, because cotton fibers are insoluble in the lysis solution, the "removing carrier" step needs to be added during the testing process to remove the cotton fibers to avoid affecting subsequent DNA extraction, elution, purification, and preservation.

[0008] Fifth, because cotton fibers are insoluble in the lysis solution, the cotton fibers will entrap some shed cells during lysis, affecting the full contact between the lysis solution and the shed cells, resulting in incomplete lysis and hindering the separation of DNA. Summary of the Invention

[0009] (I) Technical Problems to be Solved

[0010] In view of the deficiencies of the prior art, the present invention provides a solution to the problem that when laboratory testers cut the biological sample attached to the cotton swab head during the traditional collection, submission for inspection and inspection processes, it is difficult to separate the cotton swab head from the rod body and cause loss of trace biological evidence.

[0011] (II) Technical Solution

[0012] To achieve the above object, the present invention provides the following technical solution: a trace biological evidence collection and preservation set, including an outer sleeve, on which a sleeve combination is provided, and the sleeve combination is used for collecting and preserving cotton fibers attached with biological samples. The sleeve combination includes an inner filter tube, barbs, spring cards, a first groove, an inclined conical surface, a cotton swab stick, a fiber fixing sleeve, cotton fibers and a sealing ring;

[0013] The lid in the sleeve combination is threadedly sleeved on the outer sleeve, a desiccant is stored at the bottom of the outer sleeve, a connecting band is fixedly connected between the outer sleeve and the lid, the inner filter tube is arranged on a boss inside the outer sleeve, and a filter net mechanism is arranged on the bottom wall of the inner filter tube.

[0014] Preferably, the barbs are fixedly connected to the top wall of the lid, and the first groove is opened at the lower edge position of the lid.

[0015] Preferably, the two spring cards are respectively fixedly connected to the inner walls on both sides of the first groove, and positioning grooves are integrally formed on both spring cards.

[0016] Preferably, arc surfaces are integrally formed on both spring cards, and the cotton swab stick is arranged between the two positioning grooves.

[0017] Preferably, the fiber fixing sleeve is fixedly sleeved on one end of the cotton swab stick, and the fiber fixing sleeve is starfruit-shaped.

[0018] Preferably, the cotton fibers are sleeved on the fiber fixing sleeve, the inclined conical surface is opened at the opening of the outer sleeve, and a conical boss adapted to the inclined conical surface is integrally formed on the second groove.

[0019] Preferably, a second groove is opened on the conical boss, the sealing ring is arranged in the second groove, and the cotton fibers are soluble fibers and can be completely dissolved in the high-temperature pyrolysis solution.

[0020] (III) Beneficial Effects

[0021] Compared with the prior art, the present invention provides a trace biological evidence collection and preservation set, which has the following beneficial effects:

[0022] 1. The micro biological evidence collection and preservation set has barbs that can just be inserted into the gaps of the fiber fixing sleeve, enabling the inner filter tube to completely collect into the inner filter tube, thereby improving the efficiency of biological sample extraction and avoiding the problem that the cotton swab head and the rod are not easily separated when laboratory testers cut the biological sample attached to the cotton swab head in the traditional method.

[0023] 2. The micro biological evidence collection and preservation set has a fiber fixing sleeve made of silicone. This enables the fiber fixing sleeve to fit more closely to the surface of the object when wiping the biological sample with the cotton swab stick, avoiding the problem that the cotton swab is too hard and has too small a contact area with the object, resulting in low sampling efficiency in the traditional method.

[0024] 3. The micro biological evidence collection and preservation set has a fiber fixing sleeve. The cotton fibers can be evenly wound around three - quarters of the front - end surface of the fiber fixing sleeve. The scissors can quickly insert into the gap reserved at the rear one - quarter to cut the attached cotton fibers. The soluble fibers can be completely dissolved in the high - temperature lysis solution. The cotton fibers are sprayed with silver nanoparticles to play an antibacterial role, avoiding the problem that after the cotton swab wipes the biological sample and is put into the evidence packaging bag, due to the introduction of bacteria and other microorganisms in the environment during the wiping process, the biological sample is easily decomposed by microorganisms, resulting in a decrease in DNA content.

[0025] 4. The micro biological evidence collection and preservation set has an outer sleeve tube and an inner filter tube, which can enable the cotton fibers with biological samples to be well preserved, avoiding the problem that the biological samples loosely attached to the surface of the cotton swab head are easily shed during the processes of moving, transporting, and handing over after the cotton swab is put into the evidence packaging bag.

[0026] 5. The micro biological evidence collection and preservation set has cotton fibers as soluble fibers, which can be completely dissolved in the high - temperature lysis solution, avoiding the problem that in the DNA extraction step of the traditional method, because the cotton fibers are insoluble in the lysis solution, the "removing the carrier" step is required to remove the cotton fibers during the test process, and the cotton fibers will entrap some exfoliated cells during lysis, resulting in DNA loss in the "removing the carrier" step (separating and removing the cotton fibers), thus affecting the progress of the case. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 is a schematic diagram of the overall structure of the present invention;

[0028] Figure 2 is a schematic diagram of the structure of the inner filter tube of the present invention;

[0029] Figure 3 is a schematic diagram of the structure of the fiber fixing sleeve of the present invention;

[0030] Figure 4 is a schematic diagram of the structure of the positioning groove of the present invention.

[0031] In the figure: 1. Outer sleeve; 2. Lid; 3. Connecting band; 4. Inner filter tube; 5. Sealing ring; 6. Barbs; 7. Spring clip; 8. Positioning groove; 9. Arc surface; 10. First groove; 11. Oblique conical surface; 12. Cotton swab stick; 13. Fiber fixing sleeve; 14. Cotton fiber; 15. Second groove. Specific embodiments

[0032] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0033] Please refer to Figures 1-4 , the present invention provides a new technical solution: a micro biological evidence collection and preservation set, including an outer sleeve 1, on which a sleeve combination is provided. The sleeve combination is used for collecting and preserving cotton fibers attached with biological samples. The sleeve combination includes an inner filter tube 4, barbs 6, spring clips 7, a first groove 10, an oblique conical surface 11, a cotton swab stick 12, a fiber fixing sleeve 13, cotton fibers 14 and a sealing ring 5;

[0034] In the sleeve combination, the lid 2 is threadedly sleeved on the outer sleeve 1. A desiccant is stored at the bottom of the outer sleeve 1. A connecting band 3 is fixedly connected between the outer sleeve 1 and the lid 2. The inner filter tube 4 is arranged on a boss inside the outer sleeve 1, and a filter net mechanism is arranged on the bottom wall of the inner filter tube 4.

[0035] Further, the barbs 6 are fixedly connected to the top wall of the lid 2, and the first groove 10 is opened at the lower edge position of the lid 2.

[0036] Further, two spring clips 7 are respectively fixedly connected to the inner walls on both sides of the first groove 10, and positioning grooves 8 are integrally formed on both spring clips 7.

[0037] Further, arc surfaces 9 are integrally formed on both spring clips 7, and the cotton swab stick 12 is arranged between the two positioning grooves 8.

[0038] Further, the fiber fixing sleeve 13 is fixedly sleeved on one end of the cotton swab stick 12, and the fiber fixing sleeve 13 is in the shape of a carambola.

[0039] Further, the cotton fibers 14 are sleeved on the fiber fixing sleeve 13. The oblique conical surface 11 is opened at the opening of the outer sleeve 1, and a conical boss adapted to the oblique conical surface 11 is integrally formed on the second groove 15.

[0040] Further, a second groove 15 is provided on the conical boss, and the sealing ring 5 is arranged in the second groove 15. The cotton fiber 14 is a soluble fiber and can be completely dissolved in the high-temperature cracking liquid.

[0041] Further, through the provided fiber fixing sleeve 13, the cotton fiber 14 can be evenly wound around three-fourths of the front surface of the fiber fixing sleeve 13. The scissors can quickly insert into the gap reserved at the rear one-fourth to cut the attached cotton fiber 14. The soluble fiber can be completely dissolved in the high-temperature cracking liquid. Nano silver particles are sprayed on the cotton fiber 14 to play an antibacterial role, avoiding the problem that in the traditional method, after the cotton swab is used to wipe the biological sample and put into the physical evidence packaging bag, bacteria and other microorganisms in the environment are introduced during the wiping process, which easily causes the biological sample to be decomposed by microorganisms and leads to a decrease in DNA content.

[0042] Further, during collection, first pinch the end of the cotton swab stick 12 held by hand, find the microbial culture dish or microbial aggregation area to be collected, then align the cotton fiber 14 of the cotton swab stick 12 with the position to be wiped, manually slide the cotton swab stick 12, so that the cotton fiber 14 wipes the microorganisms, making the microorganisms adhere to the outer surface of the cotton fiber 14. After the wiping is completed, manually rotate the cotton swab stick 12 to check whether it is complete.

[0043] Further, the material of the provided fiber fixing sleeve 13 is silica gel, which enables the fiber fixing sleeve 13 to fit the surface of the object more closely when wiping the biological sample during the use of the cotton swab stick 12, avoiding the problem that in the traditional method, the cotton swab is too hard and the contact area with the object is too small, resulting in low sampling efficiency.

[0044] Further, after the collection is completed, first use the cotton swab stick 12 to collect the attached trace biological sample, then take out the sleeve assembly, hold it with one hand, push open the lid 2 with the thumb, insert the cotton swab head into the inner filter tube 4, press down the lid 2, so that the barb 6 inserts into the gap at the rear 1 / 4 of the cotton swab head, and then press down the tube cap while pulling out the cotton swab stick 12 backward. At this time, the barb 6 will hook the cotton fiber 14 attached to the fiber fixing sleeve 13, making all the cotton fiber 14 fall into the inner filter tube 4. Then close the lid 2. By inserting the fiber fixing sleeve 13 for wiping the biological sample into the inclined conical surface 11 during the operation, the arc surface 9 undergoes elastic deformation at this time, and the cotton swab stick 12 is just fixed between the two positioning grooves 8. Then cover the lid 2 on the outer sleeve 1 loosely, and at the same time, the cotton swab stick 12 can be pulled out backward. At this time, the barb 6 will just insert into the gap of the fiber fixing sleeve 13, realizing that all the cotton fiber 14 is collected into the inner filter tube 4, thereby improving the efficiency of biological sample extraction and avoiding the problem that it is not easy to separate the cotton swab head from the rod body when the laboratory tester cuts the biological sample attached to the cotton swab head.

[0045] Furthermore, by providing the outer sleeve 1 and the inner filter tube 4, the cotton fiber 14 with biological samples can be well preserved, avoiding the problem that the biological samples loosely attached to the surface of the cotton swab head are likely to fall off during the process of moving, transporting, and handing over after the cotton swab is put into the physical evidence packaging bag.

[0046] Furthermore, by setting the cotton fiber 14 as a soluble fiber, it can be completely dissolved in the high-temperature lysis solution, avoiding the problem that in the traditional method during the DNA extraction step, since the cotton fiber is insoluble in the lysis solution, the "removing the carrier" step is required to remove the cotton fiber during the inspection process, and the cotton fiber will entrap some exfoliated cells during lysis, resulting in DNA loss during the "removing the carrier" step (separating and removing the cotton fiber), thus affecting the progress of the case.

[0047] Structural description:

[0048] Outer sleeve 1: This is the main container of the entire sleeve assembly, used to accommodate the internal components and protect the biological samples from external contamination. Its opening is designed with an inclined conical surface 11 to facilitate the insertion and positioning of the fiber fixing sleeve 13.

[0049] Lid 2: Threadedly sleeved on the outer sleeve 1, used to close the opening of the outer sleeve 1. A barb 6 is fixedly connected to its top wall, and a first groove 10 is provided at the lower edge position for installing the spring clip 7.

[0050] Connecting band 3: Fixedly connected between the outer sleeve 1 and the lid 2, which may be used for hanging or carrying the entire sleeve assembly.

[0051] Inner filter tube 4: Arranged on the boss inside the outer sleeve 1. The function of the inner filter tube 4 is to collect and retain the biological samples attached to the cotton fiber 14.

[0052] Barb 6: Fixedly connected to the top wall of the lid 2. When the lid 2 is slightly closed, the barb 6 can be inserted into the gap of the fiber fixing sleeve 13 to prevent it from falling off.

[0053] Spring clip 7: Two spring clips 7 are respectively fixedly connected to the inner walls on both sides of the first groove 10, used to fix the cotton swab stick 12. A positioning groove 8 and an arc surface 9 are integrally formed thereon.

[0054] Positioning groove 8: Integrally formed on the spring clip 7, used to accommodate and fix the cotton swab stick 12.

[0055] Arc surface 9: Integrally formed on the spring clip 7. When the fiber fixing sleeve 13 is inserted from the inclined conical surface 11, the arc surface 9 will undergo elastic deformation to facilitate the insertion of the fiber fixing sleeve 13.

[0056] Cotton swab stick 12: It is arranged between two positioning grooves 8 and is used to support the fiber fixing sleeve 13. During use, the cotton swab stick 12 can be pulled out backward to facilitate the barbs 6 to insert into the gap of the fiber fixing sleeve 13.

[0057] Oblique conical surface 11: It is opened at the opening of the outer sleeve 1 and is adapted to the conical surface on the conical surface boss, and is used to guide the insertion and positioning of the fiber fixing sleeve 13.

[0058] Fiber fixing sleeve 13: It is fixedly sleeved on one end of the cotton swab stick and is starfruit-shaped. Its material is silica gel, which is convenient for conforming to the surface of the item during sampling, and the cotton fiber 14 can be evenly wound around three-quarters of the front surface thereof.

[0059] Cotton fiber 14: It is sleeved on the fiber fixing sleeve 13 and is used to collect and preserve biological samples. It is a soluble fiber and can be completely dissolved in the high-temperature lysis solution, avoiding the problem that cotton fibers are not easy to remove in the traditional method. At the same time, nano-silver particles are sprayed on the cotton fiber 14 to play an antibacterial role.

[0060] Second groove 15: It is opened on the conical surface boss and is used to accommodate the sealing ring 5.

[0061] Sealing ring 5: It is arranged in the second groove 15 (or may be directly installed at the joint of the outer sleeve 1 and the lid 2) and is used to ensure the sealing between the outer sleeve 1 and the lid 2.

[0062] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principle and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A kit for collecting and preserving trace biological evidence, characterized in that: The invention comprises an outer sleeve (1), on which a sleeve assembly is arranged, the sleeve assembly is used for collecting and storing cotton fibers attached with biological samples, the sleeve assembly comprises an inner filter tube (4), a barb (6), a spring card (7), a first groove (10), an inclined cone surface (11), a cotton swab stick (12), a fiber fixing sleeve (13), cotton fibers (14) and a sealing ring (5), in which a cover (2) sealing sleeve is arranged on the outer sleeve (1), a desiccant is stored at the bottom of the outer sleeve (1), a connecting belt (3) is arranged between the outer sleeve (1) and the cover (2), the inner filter tube (4) is arranged on a boss inside the outer sleeve (1), and a filter screen mechanism is arranged on the bottom wall of the inner filter tube (4).

2. The trace biological evidence collection and preservation kit according to claim 1, characterized in that: The barb (6) is fixedly connected to the top wall of the cover (2), and the first groove (10) is opened at the lower edge of the cover (2).

3. The trace biological evidence collection and preservation kit according to claim 2, characterized in that: The two spring clips (7) are respectively fixedly connected to the inner walls on both sides of the first groove (10), and positioning grooves (8) are integrally formed on the two spring clips (7).

4. The trace biological evidence collection and preservation kit according to claim 3, characterized in that: The two spring cards (7) are both integrally formed with an arc surface (9), and the cotton swab stick (12) is arranged between the two positioning grooves (8).

5. The trace biological evidence collection and preservation kit according to claim 4, characterized in that: The fiber fixing sleeve (13) is fixedly sleeved on one end of the cotton swab stick (12), and the fiber fixing sleeve (13) is in the shape of a star fruit.

6. The trace biological evidence collection and preservation kit according to claim 5, characterized in that: The cotton fiber (14) is sleeved on the fiber fixing sleeve (13), the oblique conical surface (11) is provided at the opening of the outer sleeve (1), and the second groove (15) is provided with an integrally formed conical boss adapted to the oblique conical surface (11).

7. The trace biological evidence collection and preservation kit according to claim 6, characterized in that: The conical boss is provided with a second groove (15), the sealing ring (5) is arranged in the second groove (15), and the cotton fiber (14) is a soluble fiber that can be completely dissolved in a high-temperature pyrolysis liquid.

Images