Sterile sampling device and method for cell culture

Abstract

The invention discloses a cell culture sterile sampling device and a method thereof, belongs to the technical field of cell culture, and provides the following scheme that the cell culture sterile sampling device comprises a cell culture mechanism, a sampling auxiliary mechanism is arranged on one side of the cell culture mechanism, the cell culture mechanism comprises culture equipment, a rotating assembly is arranged in the culture equipment, and the rotating assembly is arranged on the other side of the cell culture mechanism. A plurality of sealing plates and a plurality of culture dishes are arranged above the rotating assembly, and the sealing plates separate the culture dishes; the rotating assembly drives the culture dish to rotate, so that cells in the culture dish are uniformly heated, good activity of the cells is guaranteed, meanwhile, conversion is performed in the sampling cavity through rotation of the culture dish, and sealing is kept between the sealing plate and the sealing layer after conversion, so that an independent sampling space can be formed; at the moment, sampling can be carried out by opening the sampling door, independent and non-interfering sampling operation is achieved, and multiple samples can be sampled by switching among the culture dishes at a time.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, and particularly relates to a sterile sampling device and method for cell culture. Background Art

[0002] Cell culture is an important method for studying cell biology. During the cell culture process, it is often necessary to take out some living cells for research and experiments. Since cells need to be cultured in a constant temperature and sterile environment after being isolated, traditional cell culture mostly places multiple culture dishes in a sterile box for culture.

[0003] When experimental samples are needed, in order to reduce the attachment of bacteria, a series of disinfection operations are generally performed on the sampling tools before sampling, which is not only more troublesome to operate, but also when the cell culture box is opened during the sampling process, the sterile environment for stem cell culture will be damaged, affecting the growth state of stem cells. Moreover, during the sampling process, since there are more than one culture dish, it is difficult to achieve independent and non-interfering sampling. There is also a certain risk of introducing bacteria into the culture box during sampling, resulting in the cells in multiple culture dishes in the culture box losing their functions. When sampling the cells inside multiple culture dishes, this sampling method seems simple but is actually very cumbersome to operate. Frequent sterile disinfection is also a waste of scientific research time. In addition, the positions of the culture dishes inside the sterile box are generally fixed, and the heating degrees of the culture dishes at different positions in the constant temperature incubator are not uniform, easily resulting in insufficient heating or excessive heating, thereby affecting the cell activity.

[0004] In view of the above problems, the present invention document proposes a sterile sampling device and method for cell culture. Summary of the Invention

[0005] The purpose of the present invention is to solve the disadvantages that during the sampling process, since there are more than one culture dish, it is difficult to achieve independent and non-interfering sampling. When sampling the cells inside multiple culture dishes, this sampling method seems simple but is actually very cumbersome to operate. In addition, the positions of the culture dishes inside the sterile box are generally fixed, and the heating degrees of the culture dishes at different positions in the constant temperature incubator are not uniform, easily resulting in insufficient heating or excessive heating, thereby affecting the cell activity, and to propose a sterile sampling device and method for cell culture.

[0006] In order to achieve the above purpose, the present invention adopts the following technical solution:

[0007] A sterile sampling device for cell culture, comprising a cell culture mechanism, and a sampling assistance mechanism is arranged on one side of the cell culture mechanism;

[0008] The cell culture mechanism includes a culture device, inside which a rotating assembly is provided. Above the rotating assembly, there are multiple sealing plates and multiple culture dishes. The sealing plates separate the culture dishes. A sealing sampling assembly is provided on the side wall of the culture device. A sampling port is opened at a position corresponding to the sealing sampling assembly on one side of the culture device, and a sampling door corresponding to the sampling port is provided on one side of the culture device.

[0009] The sampling auxiliary mechanism includes a sampling chamber, which is fixedly installed on the culture device. An inflation assembly is provided on one side of the sampling chamber, and two annular air bags are located on both sides of the inflation assembly. The two annular air bags are connected to both ends of the inflation assembly. Two piston-type locking components are installed on the sampling chamber, and the two piston-type locking components are connected to the sampling door.

[0010] Preferably, the rotating assembly includes a motor, which is fixedly installed in the culture device. The output shaft of the motor is fixedly connected to a turntable. Above the turntable, a circular ring plate is fixedly connected. The sealing plates are fixedly connected between the circular ring plate and the turntable, and a closed space is formed between the circular ring plate, the sealing plates and the sealing sampling assembly.

[0011] Preferably, the sealing sampling assembly includes a sampling chamber, which is fixedly connected to the side wall of the culture device, and a sealing layer is fixedly connected to the sampling chamber. The sealing layer overlaps between the circular ring plate and the sealing plates.

[0012] Preferably, an ultraviolet lamp is fixedly installed in the sampling chamber, and an observation window is provided above the sampling chamber. A vacuum device is fixedly installed below the sampling chamber, and one end of the vacuum device is communicated with the sampling chamber and the culture device through a connecting pipe.

[0013] Preferably, the inflation assembly includes a piston cylinder, on which a one-way valve is installed. The piston cylinder is fixedly installed in the sampling chamber. Air outlet pipes are communicated with both sides of the piston cylinder, and the air outlet pipes are communicated with the annular air bags.

[0014] A second piston is arranged inside the piston cylinder. One side of the second piston is fixedly connected to an operating rod, and the operating rod passes through the piston cylinder and is fixedly connected to a handle.

[0015] Preferably, the two annular air bags are communicated with both ends of an exhaust pipe. The exhaust pipe passes through the sampling chamber and extends into the sampling chamber, and a valve is provided on the exhaust pipe.

[0016] Preferably, a sealing strip is installed on one side of the culture device along the edge of the sampling port, and a main switch is installed on the culture device and on one side of the sealing strip. The main switch contacts the sampling door.

[0017] Preferably, a fixing rod is installed through the sampling chamber, and a first switch and an alarm are fixedly installed at both ends of the fixing rod, and the first switch and the alarm are respectively located inside and outside the sampling chamber.

[0018] Preferably, the piston-type locking assembly includes an outer cylinder, an opening is provided at one end of the outer cylinder extending into the sampling chamber, a perforated plate is fixedly connected to the interior of the outer cylinder, a spring is fixedly connected to one side of the perforated plate, one end of the spring is fixedly connected to a first piston, the first piston is arranged in the outer cylinder, a connecting rod is fixedly connected to one side of the first piston, the connecting rod passes through the outer cylinder and is fixedly connected to a latch, the latch passes through a pin sleeve and corresponds to the first switch, the pin sleeve is mounted on a pin plate, and the pin plate is fixedly connected to the sampling door.

[0019] A method for using a cell culture sterile sampling device comprises the following steps:

[0020] S1. The motor drives the turntable to rotate, and the turntable drives the culture dish to rotate, thereby keeping the cells inside the culture dish evenly heated and maintaining good activity of the cells;

[0021] S2. When sampling, the culture personnel's arm passes through the annular airbag and enters the sampling chamber, and then reciprocates the handle, the handle drives the operating rod to move, and the operating rod drives the second piston to reciprocate, so that the second piston reciprocates through the one-way valve to intake air in one direction, and exhausts air in one direction through the outlet pipe into the annular airbag. After the annular airbag is inflated, it can be closely attached to the position of the culture personnel's arm to maintain the sealing of the sampling chamber, and at the same time, the ultraviolet lamp is used for sterilization;

[0022] S3, the sealing plate is driven to rotate and staggered with the sealing layer by the rotating assembly, and the culture device and the sampling chamber are vacuumed by the vacuum device, and the first piston overcomes the spring tension and moves by the vacuum, and the first piston drives the connecting rod to move, and the connecting rod drives the latch to move, and the latch is disengaged from the pin sleeve, and the lock of the sampling door is removed at this time, so that one end of the connecting rod contacts the first switch, and then the sealing plate is reset to keep it in close contact with the sealing layer;

[0023] S4, the culture personnel then opens the sampling door, and the main switch is closed to energize the alarm. At this time, the cells inside the culture dish are sampled by the tool. After sampling, the sample is placed in the sampling container, and then the culture dish is rotated by the rotating component to make the culture dish enter the sampling chamber. At this time, sampling is performed again. After the sampling is completed, the valve is opened to discharge the gas inside the annular airbag, and then the sample is taken out. At the same time, the spring drives the first piston to reset, so that the latch is reset and the pin sleeve is inserted to lock the sampling door;

[0024] S5. During the sampling process, if the sampling chamber leaks air, the spring drives the first piston to reset. At this time, the connecting rod moves away from the first switch, and the first switch controls the alarm to give an alarm reminder, causing the culturing personnel to stop the sampling operation and take corresponding measures for treatment.

[0025] Compared with the prior art, the present invention provides a cell culture aseptic sampling device and method, having the following beneficial effects:

[0026] 1. For the cell culture aseptic sampling device and method, the rotating assembly drives the culture dish to rotate, so that the cells inside the culture dish are evenly heated, ensuring good cell activity. At the same time, through the rotation of the culture dish, conversion is carried out in the sampling cavity. After conversion, a seal is maintained between the sealing plate and the sealing layer, thereby forming a separate sampling space. At this time, sampling can be carried out by opening the sampling door, and independent and non-interfering sampling operations can be achieved. Moreover, multiple samplings can be realized through one conversion between culture dishes.

[0027] 2. For the cell culture aseptic sampling device and method, the culturing personnel's arm passes through the annular airbag and enters the sampling chamber. At this time, the inflation assembly is operated to inflate the annular airbag, and the annular airbag closely adheres to the culturing personnel's arm to maintain tightness. At this time, the sampling chamber is evacuated by a vacuum device. Under the vacuum action, the first piston drives the connecting rod to contact the first switch, and then the sampling door is opened for sampling operation. If the sampling chamber leaks air during the sampling process, at this time, the spring drives the first piston to reset, and the first switch controls the alarm to give an alarm reminder, causing the culturing personnel to stop the subsequent sampling operation, which is convenient for the culturing personnel to take measures for treatment in a timely manner and avoid the problem of contaminating the remaining cells due to continued sampling.

[0028] 3. For the cell culture aseptic sampling device and method, the culturing personnel's arm extends into the sampling chamber, and the inflation assembly inflates the annular airbag to expand. The annular airbag closely adheres to the arm. Then, the inside of the sampling chamber is disinfected and sterilized by an ultraviolet lamp. At the same time, the vacuum device performs a vacuuming operation, causing the shaft pin of the piston-type locking component to disengage from the pin sleeve, removing the fixation of the sampling door, and then opening the sampling door for sampling operation. After sampling, the sample is placed in a sampling container, and the position of the culture dish is rotated and converted by the rotating assembly for a new round of sampling operation. Since the sampling chamber and the culturing equipment are kept sealed, a good aseptic environment is maintained. Moreover, during the sampling process, the sample can be sealed in the sampling chamber, thereby preventing the sample from being contaminated. Further, the aseptic sampling environment is multi-guaranteed. Moreover, even if contamination occurs, it can be avoided that the remaining cultured cells inside the culturing equipment are further contaminated, maintaining a good culturing environment and avoiding losses at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1Stereoscopic view of the cell culture mechanism of a cell culture sterile sampling device proposed by the present invention;

[0030] Figure 2 Stereoscopic view of the connection between the cell culture mechanism and the sampling auxiliary mechanism of a cell culture sterile sampling device proposed by the present invention;

[0031] Figure 3 Stereoscopic view of the connection between the culture device and the sampling chamber of a cell culture sterile sampling device proposed by the present invention;

[0032] Figure 4 Stereoscopic view of the cross-section of the culture device of a cell culture sterile sampling device proposed by the present invention;

[0033] Figure 5 Stereoscopic view of the cross-section of the sampling chamber of a cell culture sterile sampling device proposed by the present invention;

[0034] Figure 6 In the present invention Figure 5 Enlarged view of location A;

[0035] Figure 7 Stereoscopic view of a part of the culture device of a cell culture sterile sampling device proposed by the present invention;

[0036] Figure 8 Stereoscopic view of the connection between the annular airbag and the sampling chamber of a cell culture sterile sampling device proposed by the present invention;

[0037] Figure 9 Stereoscopic view of the connection between the piston-type locking component and the sampling door of a cell culture sterile sampling device proposed by the present invention;

[0038] Figure 10 Stereoscopic view of the cross-section of the piston-type locking component of a cell culture sterile sampling device proposed by the present invention;

[0039] Figure 11 In the present invention Figure 10 Enlarged view of location B.

[0040] In the figure: 100, cell culture mechanism; 101, culture equipment; 102, sealing plate; 103, rotating assembly; 1031, motor; 1032, turntable; 1033, annular plate; 104, culture dish; 105, sealed sampling assembly; 1051, sampling chamber; 1052, sealing layer; 106, sampling port; 107, sampling door; 108, sealing strip; 109, main switch; 200, sampling auxiliary mechanism; 201, sampling bin; 202, observation window; 203, ultraviolet lamp; 204, vacuum equipment; 205, piston-type locking component; 2051, outer cylinder; 2052, first piston; 2053, connecting rod; 2054, bolt; 2055, opening; 2056, pin sleeve; 2057, pin plate; 2058, orifice plate; 2059, spring; 206, inflation assembly; 2061, handle; 2062, operating rod; 2063, piston cylinder; 2064, second piston; 2065, one-way valve; 2066, air outlet pipe; 207, annular airbag; 208, alarm; 209, fixed rod; 210, first switch; 211, exhaust pipe; 212, valve; 213, connecting pipe. Detailed implementation mode

[0041] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments.

[0042] In the description of the present invention, it should be understood that the orientation or positional relationship indicated by the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation to the present invention.

[0043] Example 1: Refer to Figures 1 - 5 and Figure 8 , a cell culture sterile sampling device, including a cell culture mechanism 100, and a sampling auxiliary mechanism 200 is arranged on one side of the cell culture mechanism 100;

[0044] The cell culture mechanism 100 includes a culture device 101. Inside the culture device 101, a rotating assembly 103 is provided. The rotating assembly 103 includes a motor 1031. The motor 1031 is fixedly installed in the culture device 101. The output shaft of the motor 1031 is fixedly connected to a turntable 1032. Above the turntable 1032, an annular plate 1033 is fixedly connected. The sealing plate 102 is fixedly connected between the annular plate 1033 and the turntable 1032. A sealed space is formed among the annular plate 1033, the sealing plate 102, and the sealed sampling assembly 105. Through the mutual engagement among the annular plate 1033, the sampling chamber 1051, the sealing plate 102, and the sealing layer 1052, a separate sealed space can be formed, which is convenient for achieving the purpose of independent sampling. Above the rotating assembly 103, a plurality of sealing plates 102 and a plurality of culture dishes 104 are provided. The sealing plates 102 separate the culture dishes 104. On the side wall of the culture device 101, a sealed sampling assembly 105 is provided. The sealed sampling assembly 105 includes a sampling chamber 1051. The sampling chamber 1051 is fixedly connected to the side wall of the culture device 101. A sealing layer 1052 is fixedly connected to the sampling chamber 1051. The sealing layer 1052 can be closely attached to the annular plate 1033 and the sealing plate 102, so as to maintain good sealing performance. The sealing layer 1052 overlaps with the annular plate 1033 and the sealing plate 102. At the corresponding position of one side of the culture device 101 and the sealed sampling assembly 105, a sampling port 106 is opened. And on one side of the culture device 101, a sampling door 107 corresponding to the sampling port 106 is provided. By opening the sampling door 107, the cells inside the culture dish 104 can be sampled through the sampling port 106;

[0045] The sampling auxiliary mechanism 200 includes a sampling bin 201. The sampling bin 201 is fixedly installed on the culture device 101. On one side of the sampling bin 201, an inflation assembly 206 is provided, and two annular air bags 207 are located on both sides of the inflation assembly 206. The two annular air bags 207 are connected to both ends of the inflation assembly 206. Two piston-type locking assemblies 205 are installed on the sampling bin 201. The two piston-type locking assemblies 205 are connected to the sampling door 107.

[0046] In this embodiment: The motor 1031 drives the turntable 1032 to rotate. The turntable 1032 drives the culture dish 104 to rotate, so that the cells inside the culture dish 104 are evenly heated, ensuring the good activity of the cells. At the same time, through the rotation of the culture dish 104, it is switched in the sampling chamber 1051. After switching, the sealing between the sealing plate 102 and the sealing layer 1052 is maintained, so that a separate sampling space can be formed. At this time, by opening the sampling door 107, sampling can be carried out, and independent and non-interfering sampling operations can be achieved. And multiple samplings can be realized through one conversion between the culture dishes 104.

[0047] Example 2: Refer to Figures 5 - 11, a sterile cell culture sampling device, including an inflation assembly 206. The inflation assembly 206 includes a piston cylinder 2063, on which a one-way valve 2065 is installed. The one-way valve 2065 can maintain one-way air intake and prevent gas from discharging outward. The piston cylinder 2063 is fixedly installed in the sampling chamber 201. Both sides of the piston cylinder 2063 are communicated with an air outlet pipe 2066. The air outlet pipe 2066 can exhaust air unidirectionally to prevent gas from flowing back into the piston cylinder 2063. The air outlet pipe 2066 is communicated with an annular airbag 207. The two annular airbags 207 are communicated with both ends of an exhaust pipe 211. The exhaust pipe 211 passes through the sampling chamber 201 and extends into the sampling chamber 201. A valve 212 is provided on the exhaust pipe 211. By opening the valve 212, the exhaust pipe 211 is opened, so that the gas inside the annular airbag 207 can be discharged, enabling the cultivation personnel to smoothly withdraw their arms. A second piston 2064 is arranged inside the piston cylinder 2063. One side of the second piston 2064 is fixedly connected with an operating rod 2062. The operating rod 2062 passes through the piston cylinder 2063 and is fixedly connected with a handle 2061. By the handle 2061, the operating rod 2062 and the second piston 2064 can be driven to move. The reciprocating movement of the second piston 2064 can intake air through the one-way valve 2065 and exhaust air through the air outlet pipe 2066, causing the annular airbag 207 to expand and tightly adhere to the arm of the cultivation personnel, thus playing a sealing role;

[0048] A sealing strip 108 is installed on one side of the cultivation device 101. The sealing strip 108 can keep sealed with the sampling door 107, thereby maintaining the sealing performance of the cultivation device 101. The sealing strip 108 is arranged along the edge of the sampling port 106. And a main switch 109 is installed on the cultivation device 101 and on one side of the sealing strip 108. The main switch 109 contacts the sampling door 107. When the sampling door 107 is opened, the main switch 109 is closed to energize the alarm 208. When the sampling door 107 is closed, the circuit of the alarm 208 is disconnected by the main switch 109. An ultraviolet lamp 203 is fixedly installed in the sampling chamber 201. The ultraviolet lamp 203 can play a role in disinfection and sterilization, avoiding the problem of cell contamination during sampling. And an observation window 202 is arranged above the sampling chamber 201, which facilitates the cultivation personnel to perform cell sampling operations through the observation window 202. A vacuum device 204 is fixedly installed below the sampling chamber 201. One end of the vacuum device 204 is communicated with the sampling chamber 201 and the cultivation device 101 through a connecting pipe 213. A fixing rod 209 is installed through the sampling chamber 201. The fixing rod 209 can play a role in fixing the first switch 210 and the alarm 208. When the connecting rod 2053 is far away from the first switch 210, the first switch 210 is closed to realize the alarm reminder operation of the alarm 208. The two ends of the fixing rod 209 are respectively fixedly installed with the first switch 210 and the alarm 208. The first switch 210 and the alarm 208 are respectively located inside and outside the sampling chamber 201;

[0049] The piston-type locking component 205 includes an outer cylinder 2051. An opening 2055 is provided at one end of the outer cylinder 2051 that extends into the sampling chamber 201. The opening 2055 can keep the outer cylinder 2051 and the sampling chamber 201 in communication. When the sampling chamber 201 is evacuated, it can drive the first piston 2052 to move, so that the connecting rod 2053 drives the bolt 2054 to disengage from the socket 2056, facilitating the removal of the sampling door 107. A perforated plate 2058 is fixedly connected inside the outer cylinder 2051. A spring 2059 is fixedly connected to one side of the perforated plate 2058. The spring 2059 drives the first piston 2052 to reset, so that the first piston 2052 can drive the connecting rod 2053 and the bolt 2054 to reset, and the bolt 2054 can be inserted into the socket 2056 to lock the sampling door 107. Secondly, during the sampling process, the connecting rod 2053 is moved away from the first switch 210, and the first switch 210 can trigger the alarm 208 to give an alarm. One end of the spring 2059 is fixedly connected to the first piston 2052. The first piston 2052 is arranged in the outer cylinder 2051. A connecting rod 2053 is fixedly connected to one side of the first piston 2052. The connecting rod 2053 passes through the outer cylinder 2051 and is fixedly connected to the bolt 2054. The bolt 2054 passes through the socket 2056 and corresponds to the first switch 210. The socket 2056 is installed on the socket plate 2057, and the socket plate 2057 is fixedly connected to the sampling door 107.

[0050] In this embodiment: The training personnel's arm passes through the annular airbag 207 and enters the sampling chamber 201. At this time, the operating handle 2061 drives the operating rod 2062 and the second piston 2064 to reciprocate. The second piston 2064 intakes air through the one-way valve 2065 and inflates the annular airbag 207 through the air outlet pipe 2066. The annular airbag 207 is closely attached to the training personnel's arm to maintain airtightness. At this time, the sampling chamber 201 is evacuated by the vacuum device 204. Under the vacuum action, the first piston 2052 drives the connecting rod 2053 to contact the first switch 210, and at the same time the bolt 2054 disengages from the socket 2056, and then the sampling door 107 is opened. The main switch 109 is closed to keep the alarm 208 powered on, and then the sampling operation is carried out. If the sampling chamber 201 leaks air during the sampling process, at this time the spring 2059 drives the first piston 2052 to reset, and the first switch 210 controls the alarm 208 to give an alarm reminder, so that the training personnel stop the subsequent sampling operation, which is convenient for the training personnel to take measures to deal with in time and avoid the problem of contaminating the remaining cells by continued sampling.

[0051] Example 3: Refer to Figures 3 - 5 and Figure 8, a cell culture aseptic sampling device, comprising a cell culture mechanism 100. The cell culture mechanism 100 includes a culture device 101. Inside the culture device 101, a rotating assembly 103 is provided. Above the rotating assembly 103, a plurality of sealing plates 102 and a plurality of culture dishes 104 are provided. The sealing plates 102 separate the culture dishes 104. A sealing sampling assembly 105 is provided on the side wall of the culture device 101. A sampling port 106 is opened at a position corresponding to the sealing sampling assembly 105 on one side of the culture device 101, and a sampling door 107 corresponding to the sampling port 106 is provided on one side of the culture device 101;

[0052] The sampling auxiliary mechanism 200 includes a sampling chamber 201. The sampling chamber 201 is fixedly installed on the culture device 101. An inflation assembly 206 is provided on one side of the sampling chamber 201, and two annular air bags 207 are located on both sides of the inflation assembly 206. The two annular air bags 207 are connected to both ends of the inflation assembly 206. Two piston-type locking components 205 are installed on the sampling chamber 201. The two piston-type locking components 205 are connected to the sampling door 107.

[0053] In this embodiment: The culture personnel insert their arms into the sampling chamber 201, and the inflation assembly 206 inflates the annular air bags 207. The annular air bags 207 are closely attached to the arms. Then, the inside of the sampling chamber 201 is disinfected and sterilized by the ultraviolet lamp 203. At the same time, the vacuum device 204 performs a vacuum operation, so that the pin of the piston-type locking component 205 disengages from the pin sleeve 2056, removing the fixation of the sampling door 107. Then, the sampling door 107 is opened for sampling operation. After sampling, the sample is placed in a sampling container, and the position of the culture dish 104 is rotated and converted by the rotating assembly 103 for a new round of sampling operation. Since the sampling chamber 201 and the culture device 101 are kept sealed, a good aseptic environment is maintained. Moreover, during the sampling process, the sample can be sealed in the sampling chamber 201, thereby preventing the problem of sample contamination, further providing multiple guarantees for the sampling aseptic environment. Moreover, even if contamination occurs, the remaining cultured cells inside the culture device 101 can be prevented from being further contaminated, maintaining a good culture environment and avoiding losses at the same time.

[0054] A method for using a cell culture aseptic sampling device includes the following steps:

[0055] S1. Drive the turntable 1032 to rotate through the motor 1031. The turntable 1032 drives the culture dish 104 to perform a rotating motion, thereby keeping the cells inside the culture dish 104 evenly heated and maintaining good cell activity;

[0056] S2. When sampling, the culture personnel's arm passes through the annular airbag 207 to enter the sampling chamber 201, and then reciprocates the handle 2061, the handle 2061 drives the operating rod 2062 to move, the operating rod 2062 drives the second piston 2064 to reciprocate, so that the second piston 2064 reciprocates to enter the annular airbag 207 through the one-way valve 2065, and exhausts air through the outlet pipe 2066 to enter the annular airbag 207. After the annular airbag 207 is expanded, it can be closely attached to the position of the culture personnel's arm to maintain the sealing of the sampling chamber 201, and at the same time, the ultraviolet lamp 203 operates to perform sterilization;

[0057] S3, the sealing plate 102 is driven to rotate and staggered with the sealing layer 1052 by the rotating assembly 103, and the culture device 101 and the sampling chamber 201 are evacuated by the vacuum device 204. The first piston 2052 overcomes the pulling force of the spring 2059 and moves by the vacuum. The first piston 2052 drives the connecting rod 2053 to move, and the connecting rod 2053 drives the latch 2054 to move. The latch 2054 is disengaged from the pin sleeve 2056. At this time, the lock of the sampling door 107 is removed, and one end of the connecting rod 2053 contacts the first switch 210. Then the sealing plate 102 is reset to keep it in close contact with the sealing layer 1052.

[0058] S4, the culture personnel then opens the sampling door 107, and at the same time the main switch 109 is closed to energize the alarm 208. At this time, the cells inside the culture dish 104 are sampled by a tool, and after sampling, the sample is placed in a sampling container, and then the culture dish 104 is driven to rotate by the rotating assembly 103, so that the culture dish 104 enters the sampling chamber 1051, and sampling is performed again. After the sampling is completed, the valve 212 is opened to discharge the gas inside the annular airbag 207, and then the sample is taken out. At the same time, the spring 2059 drives the first piston 2052 to reset, so that the latch 2054 is reset and the pin sleeve 2056 is inserted to lock the sampling door 107;

[0059] S5. During the sampling process, the sampling chamber 201 leaks, causing the spring 2059 to drive the first piston 2052 to reset. At this time, the connecting rod 2053 is away from the first switch 210. The first switch 210 controls the alarm 208 to sound an alarm reminder, so that the culture personnel stop the sampling operation and take corresponding measures to deal with it.

[0060] The above are only preferred specific implementation modes of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical solutions and inventive concepts of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A cell culture aseptic sampling device, comprising a cell culture mechanism (100), characterized in that: A sampling auxiliary mechanism (200) is provided on one side of the cell culture mechanism (100); The cell culture mechanism (100) comprises a culture device (101), wherein a rotating assembly (103) is arranged inside the culture device (101), a plurality of sealing plates (102) and a plurality of culture dishes (104) are arranged above the rotating assembly (103), the sealing plates (102) separate the culture dishes (104), a sealing sampling assembly (105) is arranged on the side wall of the culture device (101), a sampling port (106) is provided at a position corresponding to the sealing sampling assembly (105) on one side of the culture device (101), and a sampling door (107) corresponding to the sampling port (106) is provided on one side of the culture device (101); The sampling auxiliary mechanism (200) comprises a sampling chamber (201), wherein the sampling chamber (201) is fixedly mounted on the culture device (101), an inflation component (206) and two annular air bags (207) located on both sides of the inflation component (206) are arranged on one side of the sampling chamber (201), the two annular air bags (207) are connected to both ends of the inflation component (206), and two piston-type locking components (205) are installed on the sampling chamber (201), and the two piston-type locking components (205) are connected to the sampling door (107).

2. A cell culture sterile sampling device according to claim 1, characterized in that: The rotating assembly (103) includes a motor (1031), which is fixedly installed in the culture device (101). The output shaft of the motor (1031) is fixedly connected to a turntable (1032), and a circular plate (1033) is fixedly connected above the turntable (1032). The sealing plate (102) is fixedly connected to the circular plate (1033) and the turntable (1032), and a closed space is formed between the circular plate (1033), the sealing plate (102) and the sealed sampling assembly (105).

3. A cell culture sterile sampling device according to claim 2, characterized in that: The sealed sampling assembly (105) comprises a sampling cavity (1051), wherein the sampling cavity (1051) is fixedly connected to the side wall of the culture device (101), and a sealing layer (1052) is fixedly connected to the sampling cavity (1051), and the sealing layer (1052) is overlapped with the annular plate (1033) and the sealing plate (102).

4. A cell culture sterile sampling device according to claim 3, characterized in that: An ultraviolet lamp (203) is fixedly installed in the sampling chamber (201), and an observation window (202) is arranged above the sampling chamber (201). A vacuum device (204) is fixedly installed below the sampling chamber (201), and one end of the vacuum device (204) is connected to the sampling chamber (201) and the culture device (101) through a connecting pipe (213).

5. A cell culture sterile sampling device according to claim 4, characterized in that: The inflation component (206) comprises a piston cylinder (2063), a one-way valve (2065) is installed on the piston cylinder (2063), the piston cylinder (2063) is fixedly installed in the sampling chamber (201), both sides of the piston cylinder (2063) are connected to the air outlet pipe (2066), and the air outlet pipe (2066) is connected to the annular airbag (207); A second piston (2064) is disposed inside the piston cylinder (2063), and an operating rod (2062) is fixedly connected to one side of the second piston (2064). The operating rod (2062) passes through the piston cylinder (2063) and is fixedly connected to the handle (2061).

6. A cell culture sterile sampling device according to claim 5, characterized in that: The two annular airbags (207) are connected to two ends of an exhaust pipe (211). The exhaust pipe (211) passes through the sampling chamber (201) and extends into the sampling chamber (201). A valve (212) is provided on the exhaust pipe (211).

7. A cell culture sterile sampling device according to claim 6, characterized in that: A sealing strip (108) is installed on one side of the culture device (101), and the sealing strip (108) is arranged along the edge of the sampling port (106). A main switch (109) is installed on the culture device (101) and located on one side of the sealing strip (108), and the main switch (109) is in contact with the sampling door (107).

8. A cell culture sterile sampling device according to claim 7, characterized in that: A fixing rod (209) is installed through the sampling chamber (201), and a first switch (210) and an alarm (208) are fixedly installed at both ends of the fixing rod (209), respectively. The first switch (210) and the alarm (208) are located inside and outside the sampling chamber (201), respectively.

9. A cell culture sterile sampling device according to claim 8, characterized in that: The piston-type locking assembly (205) comprises an outer cylinder (2051), one end of the outer cylinder (2051) extending into the sampling chamber (201) is provided with an opening (2055), the interior of the outer cylinder (2051) is fixedly connected to a perforated plate (2058), one side of the perforated plate (2058) is fixedly connected to a spring (2059), one end of the spring (2059) is fixedly connected to a first piston (2052), and the first piston (2052) is provided with a In the outer cylinder (2051), a connecting rod (2053) is fixedly connected to one side of the first piston (2052). The connecting rod (2053) passes through the outer cylinder (2051) and is fixedly connected to a latch (2054). The latch (2054) passes through a pin sleeve (2056) and corresponds to the first switch (210). The pin sleeve (2056) is installed on a pin plate (2057), and the pin plate (2057) is fixedly connected to the sampling door (107).

10. The method for using the cell culture sterile sampling device according to claim 9, characterized in that: The following steps are involved: S1. The motor (1031) drives the turntable (1032) to rotate, and the turntable (1032) drives the culture dish (104) to rotate, thereby maintaining uniform heating of the cells inside the culture dish (104) and maintaining good activity of the cells; S2. When sampling, the culture personnel's arm passes through the annular airbag (207) to enter the sampling chamber (201), and then reciprocates the handle (2061), the handle (2061) drives the operating rod (2062) to move, the operating rod (2062) drives the second piston (2064) to reciprocate, so that the second piston (2064) reciprocates to enter the annular airbag (207) through the one-way valve (2065) and exhausts air through the outlet pipe (2066). After the annular airbag (207) is expanded, it can be closely attached to the position of the culture personnel's arm to maintain the sealing of the sampling chamber (201), and at the same time, the ultraviolet lamp (203) operates to perform sterilization; S3, the sealing plate (102) is driven to rotate and staggered with the sealing layer (1052) through the rotating assembly (103), and then the culture device (101) and the sampling chamber (201) are evacuated through the vacuum device (204). Through the evacuation, the first piston (2052) overcomes the pulling force of the spring (2059) and moves. The first piston (2052) drives the connecting rod (2053) to move, and the connecting rod (2053) drives the latch (2054) to move. The latch (2054) is disengaged from the pin sleeve (2056). At this time, the lock of the sampling door (107) is removed, and one end of the connecting rod (2053) contacts the first switch (210). Then, the sealing plate (102) is reset to keep it in close contact with the sealing layer (1052); S4, the culture personnel then opens the sampling door (107), and at the same time the main switch (109) is closed to energize the alarm (208), and then the cells inside the culture dish (104) are sampled by a tool, and after sampling, the sample is placed in a sampling container, and then the culture dish (104) is driven to rotate by the rotating assembly (103), so that the culture dish (104) enters the sampling chamber (1051), and sampling is performed again. After the sampling is completed, the valve (212) is opened to discharge the gas inside the annular airbag (207), and then the sample is taken out, and at the same time the spring (2059) drives the first piston (2052) to reset, so that the latch (2054) is reset and inserted into the pin sleeve (2056) to lock the sampling door (107); S5. During the sampling process, the sampling chamber (201) leaks, causing the spring (2059) to drive the first piston (2052) to reset. At this time, the connecting rod (2053) is away from the first switch (210), and the first switch (210) controls the alarm (208) to sound an alarm, so that the culture personnel stop the sampling operation and take corresponding measures to deal with it.

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