Cell culture clamp and cell culture kit

Abstract

A cell culture jig for holding a cell culture bag, the cell culture jig comprising: a placement table on which the cell culture bag is placed; and a pressing member for pressing the cell culture bag placed on the placement table, the cell culture jig being provided with, on the pressing member side, a lubricating surface for reducing the frictional force acting on the contact surface between the cell culture bag placed on the placement table and the pressing surface of the pressing member for pressing the cell culture bag.

Description

Technical Field

[0001] The present invention relates to a cell culture jig and a cell culture kit. Background Art

[0002] In recent years, in the fields of pharmaceutical production, gene therapy, regenerative medicine, immunotherapy, etc., there has been a demand for culturing / differentiating cells (including tissues, microorganisms, viruses, etc.) efficiently and in large quantities in an artificial environment. In such cell culture, in order to avoid the risk of contamination, a closed-system cell culture bag is sometimes used. The cell culture bag is composed of, for example, a bag body made of a plastic film sealed at the peripheral portion, and an interface attached to the bag body.

[0003] Since the bag body of such a cell culture bag is made of a flexible material, it is easily deformed and its shape is unstable. When the bag body is deformed, the content liquid flows, and as a result, cell aggregates (clusters) are destroyed or the cells are damaged, sometimes causing an obstacle to the culture.

[0004] Therefore, Patent Document 1 describes a culture tray that sandwiches the cell culture bag between the bottom surface of the tray and a pressing plate to press it, thereby maintaining the inside of the cell culture bag in a stable state. Thereby, the flow of the content liquid caused by the deformation of the cell culture bag is suppressed.

[0005] Prior Art Documents

[0006] Patent Documents

[0007] Patent Document 1: Japanese Unexamined Patent Application Publication No. 2006-325437 Summary of the Invention

[0008] Problems to be Solved by the Invention

[0009] Thus, when clamping and pressing a cell culture bag with two members, wrinkles sometimes occur on the surface of the bag body of the cell culture bag. If wrinkles exist on the bag surface, the liquid thickness of the culture medium becomes uneven, which may have an adverse effect on cell culture, and thus is not preferable.

[0010] Therefore, the present inventors have conducted in-depth research on a cell culture jig and a cell culture kit that can easily remove wrinkles generated on the surface of a cell culture bag when the cell culture bag is held in the cell culture jig for cell culture, and as a result, completed the present invention.

[0011] Means for Solving the Problems

[0012] The cell culture jig of the present invention is a cell culture jig for holding a cell culture bag, and is configured to include: a placement table for placing the cell culture bag; and a pressing member for pressing the cell culture bag placed on the placement table. In the cell culture jig, on the pressing member side, there is provided: a lubricating surface for reducing the frictional force acting on the contact surface between the cell culture bag placed on the placement table and the pressing surface of the pressing member for pressing the cell culture bag.

[0013] In addition, the cell culture kit of the present invention has: a cell culture bag; and a cell culture jig for holding the cell culture bag. The cell culture jig includes: a placement table for placing the cell culture bag; and a pressing member for pressing the cell culture bag placed on the placement table. It is configured such that in the cell culture kit, on either one or both of the cell culture bag side and the pressing member side, there is provided: a lubricating surface for reducing the frictional force acting on the contact surface with the pressing surface of the pressing member for pressing the cell culture bag.

[0014] Advantages of the Invention

[0015] According to the present invention, when culturing cells while holding a cell culture bag in a cell culture jig, wrinkles generated on the surface of the cell culture bag can be easily removed. Brief Description of the Drawings

[0016] Figure 1 It is a schematic perspective view of a cell culture jig showing an example of the present embodiment.

[0017] Figure 2 It is a schematic explanatory view of a cell culture jig showing an example of the present embodiment.

[0018] Figure 3 It is a schematic explanatory view of a cell culture jig showing another example of the present embodiment.

[0019] Figure 4 It is a schematic perspective view of a cell culture kit showing an example of the present embodiment.

[0020] Figure 5 It is a view showing a state in which wrinkles are generated on the main body surface of a cell culture bag held in a cell culture jig. Detailed Embodiments

[0021] Hereinafter, preferred embodiments of the present invention will be described with reference to the drawings.

[0022] [First Embodiment]

[0023] First, the first embodiment of the present invention will be described.

[0024] Figure 1 FIG. 1 is a schematic perspective view of the cell culture jig 1 of the present embodiment, showing the state after the lid 5 described in detail below is opened. Figure 2 FIG. 2 is a schematic explanatory view of the cell culture jig 1 of the present embodiment, schematically showing a side view of the cell culture jig 1 in a state where the cell culture bag 10 is held inside. It should be noted that, in Figure 2 FIG. 2, in order to show the relationship of the cell culture bag 10, the placement table 2, the pressing member 3, the lid 5, and the support mechanism 51 described below, other members are appropriately omitted from the illustration.

[0025] The cell culture jig 1 of the present embodiment holds a cell culture bag 10 supplied with cells (cultured cells) to be cultured and a culture medium inside, and is used for cell culture.

[0026] Here, before describing the cell culture jig 1 of the present embodiment, a brief description of the cell culture bag 10 used in the present embodiment will be given.

[0027] The cell culture bag 10 used in the present embodiment includes a bag body 11 and an interface 12 attached to the bag body 11. The planar shape of the bag body 11 of the cell culture bag 10 can be various shapes such as a substantially rectangular shape, a square shape, a hexagonal shape, an oval shape, or a circular shape. The size of the bag body 11 is not particularly limited, and for example, it can be 50 mm to 500 mm in the longitudinal direction and 50 mm to 500 mm in the lateral direction.

[0028] The bag body 11 can be formed by laminating plastic films and heat-sealing the peripheral portions, and includes an upper surface 11a and a lower surface 11b.

[0029] Preferably, the bag body 11 is formed of a plastic film having gas permeability. Regarding the gas permeability of the plastic film, it is preferably 5000 mL / (m 2 ·day·atm) or more as measured by the gas permeability test method of JIS K 7126 at a test temperature of 37°C.

[0030] Examples of the material used for the plastic film forming the bag body 11 include thermoplastic resins such as polyethylene, polypropylene, ethylene-vinyl acetate copolymer, polyester, silicone-based elastomer, polystyrene-based elastomer, and tetrafluoroethylene-hexafluoropropylene copolymer (FEP). They can be used alone or in layers of the same or different materials, but considering the heat weldability when heat-sealing the peripheral portions, it is preferable to have a layer that functions as a sealant layer.

[0031] The interface 12 is composed of a tubular member through which the culture medium, cells, etc. can flow, and can be formed of a thermoplastic resin such as polyethylene, polypropylene, vinyl chloride, polystyrene-based elastomer, or FEP.

[0032] By holding such a cell culture bag 10 inside the cell culture jig 1 and clamping and pressing it with two members, the flow of the content liquid inside the cell culture bag 10 can be suppressed.

[0033] The cell culture jig 1 of the present embodiment includes: a mounting table 2 on which the cell culture bag 10 is mounted; and a pressing member 3 that presses the cell culture bag 10 mounted on the mounting table 2 from above.

[0034] The mounting table 2 is a plate-like member having a substantially rectangular planar shape with an area larger than that of the lower surface 11b of the cell culture bag 10, and has a mounting surface 2a for horizontally mounting the cell culture bag 10. When the cell culture bag 10 is mounted on the mounting table 2, the lower surface 11b of the cell culture bag 10 is in contact with the mounting surface 2a. The shape of the illustrated mounting surface 2a is planar, but as long as the cell culture bag 10 can be horizontally mounted, it can be appropriately changed according to the shape of the lower surface 11b of the cell culture bag 10 and is not particularly limited.

[0035] In order to confirm the state inside the cell culture bag 10, etc., a part or all of the mounting table 2 can be transparent. In this case, this part can be formed of a synthetic resin with high transparency such as polycarbonate, or glass, for example.

[0036] The pressing member 3 is a plate-like member having a substantially rectangular planar shape that matches the area of the upper surface 11a of the cell culture bag 10, and has a flat pressing surface 3a that is substantially parallel to the mounting surface 2a of the mounting table 2. The pressing surface 3a is in contact with the upper surface 11a of the cell culture bag 10 mounted on the mounting table 2 and presses the cell culture bag 10 from above.

[0037] In order to visually confirm the state inside the cell culture bag 10, etc., it is preferable that a part or all of the pressing member 3 has transparency or translucency. Considering the opening and closing of the lid 5 described later, this part can be formed of a lightweight and highly transparent polycarbonate, for example.

[0038] In the illustrated example, the pressing member 3 is supported by the lid 5 through a support mechanism 51.

[0039] The structure of the lid 5 is such that it is mounted to be openable and closable relative to the mounting table 2 through a hinge 52 or the like, can be opened relative to the mounting table 2 by approximately 90°, and is closed in a substantially parallel manner to the mounting table 2 and fixed by a lock 53a, a lock 53b, etc. In addition, in order to confirm the state inside the cell culture bag 10, etc., it is preferable that the lid 5 is configured such that the central part of the lid 5 has a wide opening and a part of the pressing member 3 is exposed from the opening, or a part of the lid 5 has transparency or the like so that the state inside the cell culture bag 10 can be visually confirmed, but the form of the lid 5 is not particularly limited.

[0040] The support mechanism 51 is composed of a guide pin and a biasing unit: the guide pin extends from the four corners of the pressing member 3 toward the lid body 5 side and penetrates the frame of the lid body 5; the biasing unit biases the pressing member 3 in a direction away from the lid body 5, and the support mechanism 51 supports the pressing member 3 and the lid body 5 in a substantially parallel state. Here, as the biasing unit, a spring member is provided between the frame of the lid body 5 and the pressing member 3. However, as the biasing unit, in addition to the spring member, for example, the repulsive force of a magnet can also be used. Thus, preferably, it is configured such that the pressing member 3 is arranged to be able to change the distance from the lid body 5 while maintaining a substantially parallel state with the lid body 5, and the pressing member 3 is biased in a direction away from the lid body 5 (i.e., in a direction in which the pressing member 3 approaches the placement table 2 when the lid body 5 is closed) by the resilience of the spring member and the repulsive force of the magnet. According to such a solution, after the cell culture bag 10 is placed on the placement table 2 and the lid body 5 is closed, even if, for example, there is unevenness in the thickness of the cell culture bag 10 due to the outflow and inflow of the culture medium, etc., the pressing member 3 can change the distance from the placement table 2 according to the thickness of the cell culture bag 10 while maintaining a state in which the cell culture bag 10 is clamped and pressed by the placement table 2 and the pressing member 3. In addition, the support mechanism 51 supports the pressing member 3 so that the distance from the lid body 5 can be changed, and having a biasing unit is also preferable when removing wrinkles on the surface of the cell culture bag 10 by the sheet member 4, and the details will be described later.

[0041] When pressing the cell culture bag 10 placed on the placement table 2 by such a pressing member 3, sometimes the bag body 11 of the cell culture bag 10 will bend and wrinkles will be generated on the upper surface 11a. This tendency is more likely to occur as the cell culture bag 10 is larger. Due to the frictional force acting on the contact surface between the surface of the bag body 11 of the cell culture bag 10 and the pressing member 3, once wrinkles are generated, they cannot be simply removed. In a state where there are wrinkles on the surface of the cell culture bag 10, the liquid thickness of the culture medium filled inside will become uneven. In addition, when replacing the culture medium, since the flow rate of the culture medium locally increases, the cells may become more likely to flow. Thus, a state where there are wrinkles on the surface of the bag body 11 of the cell culture bag 10 may have an adverse effect on cell culture, and thus is not preferable.

[0042] Therefore, the cell culture jig 1 in the present embodiment has a lubricating surface on the pressing member 3 side. More specifically, the pressing surface 3a of the pressing member 3 that contacts the upper surface 11a of the cell culture bag 10 is used as the lubricating surface. Thereby, the frictional force acting on the contact surface between the cell culture bag 10 placed on the placement table 2 and the pressing member 3 that presses the cell culture bag 10 is reduced.

[0043] The pressing surface 3a of the pressing member 3 can be roughened, for example, by sandblasting or the like to form a roughened surface with a surface roughness (Ra) of 0.01 µm to 0.70 µm, thereby forming a lubricating surface. The degree of the surface roughness can be appropriately changed according to the composition of the pressing member 3, the raw material of the bag body 11 forming the cell culture bag 10, and the like. For example, when the dynamic friction coefficient between the pressing surface 3a of the pressing member 3 and the upper surface 11a of the cell culture bag 10 is greater than 0 and 1.0 or less, preferably 0.05 or more and 1.0 or less, more preferably 0.1 or more and 0.7 or less, and further preferably 0.3 or more and 0.7 or less, the number of swings of the pressing member 3 required to remove the wrinkles on the surface of the cell culture bag 10 can be reduced, which is therefore preferable.

[0044] According to such a cell culture jig 1, when the cell culture bag 10 is placed on the placement table 2, the lid 5 is closed, and the cell culture bag 10 is pressed by the pressing member 3, even if wrinkles occur on the upper surface 11a of the cell culture bag 10, the wrinkles can be easily removed in the following manner.

[0045] After confirming the wrinkles on the surface of the bag body 11, the pressing member 3 is swung in a direction away from the surface of the bag body 11 of the cell culture bag 10. In this way, as the contents such as the culture medium in the cell culture bag 10 slightly shake, the upper surface 11a of the bag body 11 moves, the pressing surface 3a of the pressing member 3 functions as a lubricating surface with slidability, the upper surface 11a of the cell culture bag 10 slides on the flat pressing surface 3a, and the upper surface 11a becomes flat along the shape of the pressing surface 3a. In this way, the wrinkles on the surface of the bag body 11 of the cell culture bag 10 can be removed.

[0046] When the pressing member 3 is swung in a direction away from the surface of the bag body 11, this situation can also move together with the lid 5 that supports the pressing member 3. Or, when the pressing member 3 is supported by the above-mentioned support mechanism 51 so that the interval between it and the lid 5 can be changed, and a biasing unit biases it away from the lid 5, even after the lid 5 is locked to the placement table 2 by the buckle 53a, the buckle 53b, etc., the pressing member 3 can be swung by pushing it up in a manner close to the lid 5 by placing a finger or the like on the edge side of the pressing member 3, which is therefore preferable.

[0047] [Second Embodiment]

[0048] Next, a second embodiment of the present invention will be described.

[0049] Figure 3FIG. 0 is a schematic explanatory view showing the cell culture jig 1 of the present embodiment, schematically showing a side view of the cell culture jig 1 in a state where the cell culture bag 10 is held inside. It should be noted that, similar to Figure 2 similarly, in Figure 3 , in order to show the relationality of the cell culture bag 10, the mounting table 2, the pressing member 3, the sheet member 4, the lid body 5, and the support mechanism 51 described below, other members are appropriately omitted from the illustration.

[0050] In the above-described first embodiment, it is configured such that the pressing surface 3a of the pressing member 3 that contacts the upper surface 11a of the cell culture bag 10 is used as a lubricating surface, and on the side of the pressing member 3, there is provided: a lubricating surface that reduces the frictional force acting on the contact surface between the cell culture bag 10 placed on the mounting table 2 and the pressing member 3 that presses the cell culture bag 10. In contrast, in the present embodiment, it is configured such that a sheet member 4 having a lubricating surface is interposed between the cell culture bag 10 and the pressing member 3, and on the side of the pressing member 3, there is provided: a lubricating surface that reduces the frictional force acting on the contact surface between the cell culture bag 10 placed on the mounting table 2 and the pressing member 3 that presses the cell culture bag 10, rather than using the pressing surface 3a of the pressing member 3 as the lubricating surface.

[0051] The sheet member 4 is a flexible sheet that can be attached to the pressing surface 3a of the pressing member 3, and preferably has transparency or translucency in order to visually confirm the state inside the cell culture bag 10 and the like. The sheet member 4 can be formed of a thermoplastic resin, but there is no particular limitation on the material used for the sheet member 4 as long as the bag contact surface 4a can be made into a lubricating surface as described below.

[0052] The sheet member 4 in the present embodiment has a bag contact surface 4a that contacts the upper surface 11a of the cell culture bag 10 on the side opposite to the surface facing the pressing surface 3a of the pressing member 3. In the present embodiment, this bag contact surface 4a is used as the lubricating surface.

[0053] The bag contact surface 4a of the sheet member 4 can be formed into a lubricating surface, for example, by performing a roughening treatment such as sandblasting to form a roughened surface with a surface roughness (Ra) of 0.01 µm to 0.70 µm.

[0054] Alternatively, the sheet member 4 can also be formed using a material with high self-lubricity to make its surface a lubricating surface. Examples of such materials include polytetrafluoroethylene, polyacetal, ultra-high molecular weight polyethylene, MC nylon, etc.

[0055] Alternatively, the bag contact surface 4a of the sheet member 4 may also be formed to have an outer layer imparted with slidability. In this case, the outer layer of the sheet member 4 may contain a lubricant such as silicone, surfactant, petroleum wax, synthetic paraffin, liquid paraffin, fatty acid ester, amide compound, etc. As the material constituting the cell culture bag 10, even if it is a material that may affect the cultured cells and cannot be used, it is no problem for use in the sheet member 4. Therefore, various materials can be used to impart the desired physical properties to the sheet member 4.

[0056] Thus, when the bag contact surface 4a of the sheet member 4 is configured such that the coefficient of kinetic friction with the upper surface 11a of the cell culture bag 10 is greater than 0 and 1.0 or less, preferably 0.05 or more and 1.0 or less, more preferably 0.1 or more and 0.7 or less, and further preferably 0.3 or more and 0.7 or less, the number of swings of the pressing member 3 required to remove the wrinkles on the surface of the cell culture bag 10 can be reduced, which is therefore preferable.

[0057] According to the cell culture jig 1 of the present embodiment, when the cell culture bag 10 is placed on the placement table 2, the lid 5 is closed, and the cell culture bag 10 is pressed by the pressing member 3, even if wrinkles form on the upper surface 11a of the cell culture bag 10, the wrinkles on the surface of the bag body 11 of the cell culture bag 10 can be easily removed. That is, after the wrinkles are confirmed, the pressing member 3 is swung in a direction away from the surface of the bag body 11 of the cell culture bag 10 in the same manner as in the first embodiment. In this way, the bag contact surface 4a of the sheet member 4 that adheres to the pressing surface 3a of the pressing member 3 functions as a lubricating surface with slidability, and the upper surface 11a side of the cell culture bag 10 slides on the flat pressing surface 3a, and the surface of the bag body 11 becomes flat along the shape of the pressing surface 3a, and the wrinkles on the surface of the bag body 11 of the cell culture bag 10 can be removed.

[0058] This embodiment is different from the first embodiment in the above aspect, but other structures are the same as those of the first embodiment, so repeated descriptions are omitted.

[0059] As described above, an example of the cell culture jig 1 having a lubricating surface on the pressing member 3 side is given, which reduces the frictional force acting on the contact surface between the cell culture bag 10 placed on the placement table 2 and the pressing surface 3a of the pressing member 3 that presses the cell culture bag 10. However, the present invention is not limited to the above embodiment. A lubricating surface may also be provided on the cell culture bag 10 side, and an example thereof is shown below.

[0060] [Third Embodiment]

[0061] A description will be given of the third embodiment of the present invention.

[0062] Figure 4FIG. 0 is a schematic perspective view showing the cell culture kit 100 of the present embodiment, showing the state after the lid 5 is opened. The cell culture kit 100 includes: a cell culture bag 10; and a cell culture jig 1 including a placement table 2 for placing the cell culture bag and a pressing member 3 for pressing the cell culture bag 10 placed on the placement table 2.

[0063] In the above-described first embodiment, it is configured such that the pressing surface 3a of the pressing member 3 that contacts the upper surface 11a of the cell culture bag 10 is used as a lubricating surface, and on the side of the pressing member 3, there is provided: a lubricating surface that reduces the frictional force acting on the contact surface between the cell culture bag 10 placed on the placement table 2 and the pressing member 3 that presses the cell culture bag 10. In contrast, in the present embodiment, it is configured such that the upper surface 11a of the cell culture bag 10 placed on the cell culture jig 1 is used as a lubricating surface, and on the side of the cell culture bag 10, there is provided: a lubricating surface that reduces the frictional force acting on the contact surface between the cell culture bag 10 placed on the placement table 2 and the pressing member 3 that presses the cell culture bag 10, rather than using the pressing surface 3a of the pressing member 3 as a lubricating surface.

[0064] As described above, the upper surface 11a of the cell culture bag 10 included in the cell culture kit of the present embodiment can be formed of a thermoplastic resin such as polyethylene, polypropylene, ethylene-vinyl acetate copolymer, polyester, silicone-based elastomer, polystyrene-based elastomer, tetrafluoroethylene-hexafluoropropylene copolymer (FEP), etc.

[0065] The upper surface 11a of the cell culture bag 10 can be formed into a lubricating surface by, for example, performing a roughening treatment such as sandblasting to form a roughened surface with a surface roughness (Ra) of 0.01 µm to 0.70 µm of the pressing member 3. It should be noted that the degree of the surface roughness can be appropriately changed according to the configuration of the pressing member 3, the raw material of the bag body 11 forming the cell culture bag 10, etc.

[0066] Alternatively, the upper surface 11a of the cell culture bag 10 can be formed using a material with high self-lubricity to make its surface a lubricating surface. Examples of such materials include polytetrafluoroethylene and ultra-high molecular weight polyethylene.

[0067] Thus, when the kinetic friction coefficient between the pressing surface 3a of the pressing member 3 and the upper surface 11a of the cell culture bag 10 is greater than 0 and 1.0 or less, preferably 0.05 or more and 1.0 or less, more preferably 0.1 or more and 0.7 or less, and further preferably 0.3 or more and 0.7 or less, the number of swings of the pressing member 3 required to remove the wrinkles on the surface of the cell culture bag 10 can be reduced, which is therefore preferable.

[0068] According to such a cell culture kit 100, when the cell culture bag 10 is placed on the placement table 2, the lid 5 is closed, and the cell culture bag 10 is pressed by the pressing member 3, even if the upper surface 11a of the cell culture bag 10 wrinkles, the wrinkles on the surface of the bag body 11 of the cell culture bag 10 can be easily removed. That is, after confirming the wrinkles, the pressing member 3 is swung in the same manner as in the first embodiment so as to move away from the surface of the bag body 11 of the cell culture bag 10. In this way, the upper surface 11a of the cell culture bag 10 functions as a lubricating surface with slidability, the upper surface 11a of the cell culture bag 10 slides on the flat pressing surface 3a, and the surface of the bag body 11 becomes flat along the shape of the pressing surface 3a, and the wrinkles on the surface of the bag body 11 of the cell culture bag 10 can be removed.

[0069] This embodiment is different from the first embodiment in the above aspect, but other structures are the same as those of the first embodiment, so repeated descriptions are omitted.

[0070] [Fourth Embodiment]

[0071] Next, a fourth embodiment of the present invention will be described.

[0072] In the above-described second embodiment, it is configured that a sheet member 4 is interposed between the cell culture bag 10 and the pressing member 3, the sheet member 4 is attached to the pressing surface 3a of the pressing member 3, and the bag contact surface 4a of the sheet member 4 that contacts the upper surface 11a of the cell culture bag 10 is used as a lubricating surface, and on the pressing member side, there is provided: a lubricating surface that reduces the frictional force acting on the contact surface between the cell culture bag 10 placed on the placement table 2 and the pressing surface 3a of the pressing member 3. In contrast, in this embodiment, it is configured that the sheet member 4 is attached to the upper surface 11a of the cell culture bag 10, and the pressing member facing surface 4b of the sheet member 4 that faces the pressing surface 3a of the pressing member 3 is used as a lubricating surface, and on the cell culture bag 10 side, there is provided: a lubricating surface that reduces the frictional force acting on the contact surface between the cell culture bag 10 placed on the placement table 2 and the pressing surface 3a of the pressing member 3 (see Figure 3 ).

[0073] According to such a cell culture kit 100, when the cell culture bag 10 is placed on the placement table 2, the lid 5 is closed, and the cell culture bag 10 is pressed by the pressing member 3, even if wrinkles form on the upper surface 11a of the cell culture bag 10, the wrinkles on the surface of the bag body 11 of the cell culture bag 10 can be easily removed. That is, after confirming the wrinkles, the pressing member 3 is swung in a direction away from the surface of the bag body 11 of the cell culture bag 10 in the same manner as in the first embodiment. In this way, the pressing member facing surface 4b of the sheet member 4 that adheres to the upper surface 11a of the cell culture bag 10 functions as a lubricating surface with slidability, and the upper surface 11a side of the cell culture bag 10 slides on the flat pressing surface 3a, and the surface of the bag body 11 becomes flat along the shape of the pressing surface 3a, and the wrinkles on the surface of the bag body 11 of the cell culture bag 10 can be removed.

[0074] This embodiment is different from the second embodiment in the above aspect, but other structures are the same as those of the second embodiment, so repeated descriptions are omitted.

[0075] In this way, by providing a lubricating surface on either the cell culture bag 10 side or the pressing member 3 side to reduce the frictional force acting on the contact surface between the cell culture bag 10 placed on the placement table 2 and the pressing surface 3a of the pressing member 3 that presses the cell culture bag 10, when the cell culture bag 10 is held inside the cell culture jig 1, the wrinkles generated on the surface of the bag body 11 of the cell culture bag 10 can be easily removed. It should be noted that, of course, a lubricating surface can also be provided on both the cell culture bag 10 side and the pressing member 3 side to reduce the frictional force acting on the contact surface between the cell culture bag 10 placed on the placement table 2 and the pressing surface 3a of the pressing member 3 that presses the cell culture bag 10.

[0076] Examples

[0077] Hereinafter, specific examples will be listed to explain the present invention in more detail.

[0078] [Example 1]

[0079] The pressing surface 3a of the pressing member 3 made of polycarbonate was subjected to sandblasting using a shot material (manufactured by Fuji Seisakusho Co., Ltd.) in which glass abrasive grains with an average particle size of 11 µm were kneaded into a rubber core body with an average particle size of 350 µm, so that the surface roughness (Ra) was 0.118 µm. The transparency (Haze) of the pressing member 3 was 36.22. The bag body 11 of the used cell culture bag 10 was molded from polyethylene, and the planar shape of the bag body 11 was 26.7 cm in length × 36.4 cm in width. The dynamic friction coefficient between the pressing surface 3a of the pressing member 3 and the upper surface 11a of the cell culture bag 10 was 0.39.

[0080] In Figure 1In the cell culture jig 1 shown, when the cell culture bag 10 filled with about 400 ml of culture medium was placed on the cell culture jig 1 having the pressing member 3 with the above structure, the lid 5 was closed and locked by the buckle, wrinkles were generated on the surface of the bag body 11 of the cell culture bag 10. Figure 5 It is a diagram showing a state in which wrinkles are generated on the surface of the bag body 11 of the cell culture bag 10 held inside the cell culture jig 1 and pressed by the pressing member 3. Then, when the finger was placed on the edge side of the pressing member 3 and the pressing member 3 was swung once in a manner approaching the lid 5, it was confirmed that the wrinkles were removed.

[0081] [Example 2]

[0082] Shot peening was performed using a shot material (manufactured by Fuji Seisakusho Co., Ltd.) in which glass abrasive grains with an average particle size of 0.5 µm were kneaded into a rubber core body with an average particle size of 350 µm, so that the surface roughness (Ra) of the pressing surface 3a of the pressing member 3 was 0.018 µm. Except for this, the cell culture jig 1 and the cell culture bag 10 were prepared in the same manner as in Example 1. The transparency (Haze) of the pressing member 3 was 4.58, and the dynamic friction coefficient between the pressing surface 3a of the pressing member 3 and the upper surface 11a of the cell culture bag 10 was 0.61.

[0083] Similar to Example 1, when the cell culture bag 10 was held inside the cell culture jig 1 of Example 2, wrinkles were generated on the surface of the bag body 11 of the cell culture bag 10. Then, when the finger was placed on the edge of the pressing member 3 and the pressing member 3 was swung twice in a manner approaching the lid 5, it was confirmed that the wrinkles were removed.

[0084] [Example 3]

[0085] Shot peening was performed using a shot material (manufactured by Fuji Seisakusho Co., Ltd.) in which glass abrasive grains with an average particle size of 1.2 µm were kneaded into a rubber core body with an average particle size of 350 µm, so that the surface roughness (Ra) of the pressing surface 3a of the pressing member 3 was 0.037 µm. Except for this, the cell culture jig 1 and the cell culture bag 10 were prepared in the same manner as in Example 1. The transparency (Haze) of the pressing member 3 was 7.76, and the dynamic friction coefficient between the pressing surface 3a of the pressing member 3 and the upper surface 11a of the cell culture bag 10 was 0.55.

[0086] Similar to Example 1, when the cell culture bag 10 was held inside the cell culture jig 1 of Example 3, wrinkles were generated on the surface of the bag body 11 of the cell culture bag 10. Then, when the finger was placed on the edge of the pressing member 3 and the pressing member 3 was swung twice in a manner approaching the lid 5, it was confirmed that the wrinkles were removed.

[0087] [Example 4]

[0088] Shot peening was performed using a shot peening material (manufactured by Fuji Seisakusho Co., Ltd.) in which glass abrasive grains with an average particle size of 4 µm were kneaded into a rubber core body with an average particle size of 350 µm, so that the surface roughness (Ra) of the pressing surface 3a of the pressing member 3 was 0.062 µm. Other than this, the cell culture jig 1 and the cell culture bag 10 were prepared in the same manner as in Example 1. The transparency (Haze) of the pressing member 3 was 17.42, and the dynamic friction coefficient between the pressing surface 3a of the pressing member 3 and the upper surface 11a of the cell culture bag 10 was 0.41.

[0089] In the same manner as in Example 1, when the cell culture bag 10 was held inside the cell culture jig 1 of Example 4, wrinkles were generated on the surface of the bag body 11 of the cell culture bag 10. Then, when the finger was placed on the edge of the pressing member 3 and the pressing member 3 was swung 7 times in a manner to approach the lid body 5, it was confirmed that the wrinkles were removed.

[0090] [Example 5]

[0091] Shot peening was performed using a shot peening material (manufactured by Fuji Seisakusho Co., Ltd.) in which glass abrasive grains with an average particle size of 20 µm were kneaded into a rubber core body with an average particle size of 350 µm, so that the surface roughness (Ra) of the pressing surface 3a of the pressing member 3 was 0.254 µm. Other than this, the cell culture jig 1 and the cell culture bag 10 were prepared in the same manner as in Example 1. The transparency (Haze) of the pressing member 3 was 74.4, and the dynamic friction coefficient between the pressing surface 3a of the pressing member 3 and the upper surface 11a of the cell culture bag 10 was 0.44.

[0092] In the same manner as in Example 1, when the cell culture bag 10 was held inside the cell culture jig 1 of Example 5, wrinkles were generated on the surface of the bag body 11 of the cell culture bag 10. Then, when the finger was placed on the edge of the pressing member 3 and the pressing member 3 was swung 8 times in a manner to approach the lid body 5, it was confirmed that the wrinkles were removed.

[0093] [Example 6]

[0094] Shot peening was performed using a shot peening material (manufactured by Fuji Seisakusho Co., Ltd.) in which glass abrasive grains with an average particle size of 40 µm were kneaded into a rubber core body with an average particle size of 350 µm, so that the surface roughness (Ra) of the pressing surface 3a of the pressing member 3 was 0.658 µm. Other than this, the cell culture jig 1 and the cell culture bag 10 were prepared in the same manner as in Example 1. The transparency (Haze) of the pressing member 3 was 83.26, and the dynamic friction coefficient between the pressing surface 3a of the pressing member 3 and the upper surface 11a of the cell culture bag 10 was 0.43.

[0095] Similar to Example 1, when the cell culture bag 10 was held inside the cell culture jig 1 of Example 6, wrinkles were generated on the surface of the bag body 11 of the cell culture bag 10. Then, when the finger was placed on the edge of the pressing member 3 and the pressing member 3 was swung 12 times in a manner approaching the lid 5, it was confirmed that the wrinkles were removed.

[0096] [Comparative Example]

[0097] Except that the pressing surface 3a of the pressing member 3 was not subjected to sandblasting, the cell culture jig 1 and the cell culture bag 10 were prepared in the same manner as in Example 1. The surface roughness (Ra) of the pressing surface 3a of the pressing member 3 was 0.004 µm, the transparency (Haze) of the pressing member 3 was 0.72, and the dynamic friction coefficient between the pressing surface 3a of the pressing member 3 and the upper surface 11a of the cell culture bag 10 was 2.02.

[0098] Similar to Example 1, when the cell culture bag 10 was held inside the cell culture jig 1 of the comparative example, wrinkles were generated on the surface of the bag body 11 of the cell culture bag 10. Then, the finger was placed on the edge of the pressing member 3 and the pressing member 3 was swung 30 times in a manner approaching the lid 5, but the wrinkles were not removed.

[0099] As described above, the preferred embodiments have been used to illustrate the present invention. However, it is obvious that the present invention is not limited to the above-described embodiments, and various modifications can be made within the scope of the present invention.

[0100] For example, in the present embodiment, it is configured to reduce the frictional force between the upper surface 11a of the cell culture bag 10 and the pressing member 3. However, a lubricating surface may also be provided on the side of the mounting table 2 or the cell culture bag 10 to reduce the frictional force acting on the contact surface between the cell culture bag 10 and the mounting table 2, so as to remove the wrinkles generated on the lower surface 11b of the cell culture bag 10. The mounting surface 2a may be used as the lubricating surface, or the lower surface 11b of the cell culture bag 10 may be used as the lubricating surface. In addition, a sheet member 4 having a lubricating surface may be interposed between the cell culture bag 10 and the mounting table 2. In this case, in order to remove the wrinkles generated on the lower surface 11b of the cell culture bag 10, it is only necessary to lift the bag body 11 of the cell culture bag 10 with a finger and swing it.

[0101] In addition, in the illustrated example, the shape of the lower surface 11b of the bag body 11 is the same as that of the upper surface 11a, i.e., flat. However, a plurality of concave portions that respectively form cell culture portions may also be formed on the lower surface 11b. In this case, in order to avoid the collapse and deformation of the concave portions and prevent the cells in the concave portions from flowing out, openings and concave portions may also be formed on the mounting surface 2a of the mounting table 2 in a shape that respectively accommodates the plurality of concave portions formed on the lower surface 11b of the cell culture bag 10, and the concave portions of the lower surface 11b may be supported in a non-contact manner.

[0102] Explanation of Reference Numerals

[0103] 1: Cell culture jig;

[0104] 2: Mounting table;

[0105] 3: Pressing member;

[0106] 4: Sheet member;

[0107] 5: Cover;

[0108] 10: Cell culture bag.

Claims

1. A cell culture fixture, characterized in that: The invention is a cell culture clamp for holding a cell culture bag, and the cell culture clamp comprises: a loading platform for loading the cell culture bag; and A pressing member presses the cell culture bag placed on the mounting table, The cell culture jig includes a lubricating surface on the pressing member side to reduce frictional force acting on a contact surface between the cell culture bag placed on the mounting table and a pressing surface of the pressing member pressing the cell culture bag.

2. The cell culture fixture according to claim 1, wherein: A sheet member having the lubricating surface is interposed between the cell culture bag and the pressing member.

3. The cell culture fixture according to claim 1 or 2, wherein: The lubricating surface is a roughened surface subjected to a roughening treatment.

4. The cell culture fixture according to claim 3, wherein: The surface roughness Ra of the roughened surface is 0.01µm to 0.70µm.

5. The cell culture fixture according to claim 2, wherein: The sheet member is made of a self-lubricating material.

6. A cell culture kit, characterized in that: The cell culture kit comprises: Cell culture bags; and A cell culture fixture is used to hold the cell culture bag, and the cell culture fixture comprises: a loading table, which loads the cell culture bag; and a pressing member for pressing the cell culture bag placed on the placing table, In the cell culture kit, one or both of the cell culture bag side and the pressing member side include a lubricating surface that reduces friction acting on a contact surface between the cell culture bag placed on the mounting table and a pressing surface of the pressing member pressing the cell culture bag.

7. The cell culture kit according to claim 6, wherein A sheet member having the lubricating surface is interposed between the cell culture bag and the pressing member.

8. The cell culture kit according to claim 6 or 7, wherein The lubricating surface is a roughened surface subjected to a roughening treatment.

9. The cell culture kit according to claim 8, wherein The surface roughness Ra of the roughened surface is 0.01µm to 0.70µm.

10. The cell culture kit according to claim 7, wherein The sheet member is made of a self-lubricating material.

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