Container mycorrhizal hardwood cutting seedling raising method for lycium barbarum

Abstract

The invention provides a container mycorrhizal hardwood cutting seedling raising method for lycium barbarum, which is characterized in that glomus mosseae and glomus intraradices are used as arbuscular mycorrhizal fungi for lycium barbarum nursery stock breeding, a mycorrhizal agent and clay are mixed to prepare slurry for lycium barbarum hardwood cutting, the method is a novel method combining a mycorrhizal technology and traditional cutting, and the method has the advantages that the mycorrhizal technology and the traditional cutting technology are combined; the rooting rate of the cutting slips can reach 95% or above, and the survival rate (10% or above), disease resistance and growth performance of the Chinese wolfberry cutting seedlings are remarkably improved. According to the method, container mycorrhizal cultivation of lycium barbarum is adopted, the seedling raising period can be shortened, the seedling survival rate is increased, the seedling raising cost is reduced, and the income of seedling farmers is increased.

Description

Technical Field

[0001] The present invention belongs to the technical field of Lycium barbarum seedling cultivation, and particularly relates to a method for culturing mycorrhizal hardwood cuttings of leaf Lycium barbarum in containers. Background Art

[0002] Leaf Lycium barbarum, also known as large-leaf Lycium barbarum, Lycium barbarum vegetable, and Lycium barbarum tooth vegetable, with the scientific name Lycium chinense Mill., is a variety of Lycium barbarum with relatively large leaves, and its tender stem tips and leaves are used for vegetable consumption. Leaf Lycium barbarum is rich in betaine, rutin, various amino acids (such as aspartic acid, glutamic acid), vitamins (A, C, E, B group), and trace elements such as calcium, iron, and zinc. It has the effects of antioxidant and anti-aging, protecting the liver and improving eyesight, regulating blood pressure, blood sugar and blood lipid, moistening the lungs and relieving cough, etc. It can also be used in traditional Chinese medicine to tonify the liver and kidney, clear heat and detoxify, soothe the nerves and aid sleep, and has important medicinal value. The existing methods for cultivating leaf Lycium barbarum mainly include cutting propagation or root division / root suckering propagation, and generally have the problems of low production efficiency and low survival rate.

[0003] Mycorrhiza is a symbiotic body formed by arbuscular mycorrhizal fungi (AMF) and plant roots. The formation of mycorrhiza can improve the survival rate of plants, promote the growth of plants, enhance the abilities of plants to resist drought, salinity, diseases, heavy metal pollution, oil pollution, etc., and at the same time promote the absorption of mineral nutrients by plants, especially the absorption of phosphorus. As a new type of biological fertilizer, mycorrhizal fungi have good effects in promoting the growth of plants under stress and restoring degraded land, especially in aspects such as seedling transplantation and afforestation under stress. Summary of the Invention

[0004] The technical problem to be solved by the present invention is to provide a method for culturing mycorrhizal hardwood cuttings of leaf Lycium barbarum in containers in view of the deficiencies of the above-mentioned prior art. By combining mycorrhizal technology with traditional cutting methods, the rooting rate of cuttings, the survival rate, disease resistance and growth performance of Lycium barbarum cuttings are significantly improved.

[0005] To solve the above technical problems, the technical solution adopted by the present invention is: a method for culturing mycorrhizal hardwood cuttings of leaf Lycium barbarum in containers, comprising the following steps: S1. Selection of Seedling Raising Land Select a place relatively close to the afforestation land as the seedling raising land for Lycium barbarum containers; S2. Preparation of Mycorrhizal Agent Mud Inoculate Glomus mosseae and Glomus intraradices onto corn and clover seeds respectively, and carry out agent propagation in a river sand substrate to obtain a mycorrhizal agent for Lycium barbarum hardwood cuttings; mix the mycorrhizal agent with clay and add water to obtain mycorrhizal agent mud; S3. Treatment of Cuttings S301, Cutting Collection: Select healthy Lycium barbarum L. mother trees without pests and diseases. Collect cuttings in early March before the branches sprout. S302, Cutting Preparation: Cut the collected cuttings into lengths of 8 cm - 10 cm, retaining 3 - 4 bud points. Cut the lower end of the cutting into a "horseshoe shape" at a 45-degree angle, and cut the upper end flat. Discard the part of the cutting with a thickness ≤ 0.4 cm to obtain the cuttings. S303, Cutting Treatment: Tie the prepared cuttings in bundles with the inclined ends facing down. First, soak them in a potassium permanganate solution with a mass fraction of 0.1% for disinfection, then rinse with clean water, and then soak them in a rooting agent solution. S304, Sand Storage and Root Induction: Place the treated cuttings with the inclined ends facing up on the ground, cover them with 10 - 20 cm of wet sand, control the environmental temperature at 22 - 30 °C, and induce roots for 5 - 10 days. S4, Preparation of Containers and Substrates Select nutrient bags or plug trays as containers. The substrate ratio is: 65% coconut coir, 10% peat, 20% perlite, and 5% vermiculite. Mix them evenly and spray with an 800-fold aqueous solution of 50% carbendazim for disinfection, and then fill them into nutrient bags or plug trays for later use. S5, Dipping in Slurry and Cuttage When obvious callus begins to appear at the inclined part of the cutting, immerse the root of the cutting into the mycorrhizal agent slurry obtained in S2. The height of the slurry adhered to the bottom of the cutting is 3 cm - 5 cm, and the thickness of the slurry covering the cut part is 2 mm - 3 mm, and then directly conduct cuttage. S6, Management after Cuttage After cuttage, manage the temperature and humidity, conduct regular pest and disease control and foliar fertilization to promote growth, and conduct ventilation hardening and seedling pruning according to the situation.

[0006] Preferably, in S1, the annual average sunshine hours of the Lycium barbarum L. container seedling raising land ≥ 2250 h, the annual average temperature ≥ 6.0 °C, the day-night temperature difference is 13 °C - 17 °C, the accumulated temperature of activities ≥ 10 °C ≥ 1900 °C, there is a fresh water source, and the salinity of the water ≤ 2 g / L.

[0007] Preferably, the method for multiplying mycorrhizal agents in S2 is as follows: In late May, mix Glomus mosseae disinfected with 0.5% potassium permanganate solution with pure river sand evenly at a mass ratio of 1:5 to 1:10 to make a sand bed with a thickness of 10 cm, and plant 8 to 10 corn seeds per square meter of the sand bed; mix Glomus intraradices disinfected with 0.5% potassium permanganate solution with pure river sand evenly at a mass ratio of 1:5 to 1:10 to make a sand bed with a thickness of 10 cm, and plant 180 to 200 clover seeds per square meter of the sand bed; after seedlings grow in the two culture media, cultivate them with Hoagland nutrient solution. After 5.5 months of inoculation, cut off the above-ground parts of the host plants, corn and clover, collect the river sand of the two fungal culture media together, cut the corn roots and clover roots into 1 cm to 2 cm and mix them evenly with the river sand, and collect them, which is the mycorrhizal agent for the propagation of hard branch cuttings of wolfberry.

[0008] Preferably, the clay selected in S2 is fine and impurity-free clay with a particle size ≤ 0.002 mm; the preparation method of the mycorrhizal agent slurry is: mix the mycorrhizal agent and clay at a mass ratio of 1:3 to 1:5 to form mycorrhizal soil, and then mix the mycorrhizal soil and water at a mass ratio of 1:5 to 1:7 to prepare the mycorrhizal agent slurry.

[0009] Preferably, the wolfberry variety is all the varieties of leaf wolfberry that have been approved.

[0010] Preferably, the cutting collection in S301 is to collect one-year-old lignified hard branches with a diameter of 0.5 cm to 0.8 cm and a length of 50 cm to 100 cm to obtain cuttings.

[0011] Preferably, the method for cutting treatment in S303 is: tie 50 cuttings made into a bundle, with the inclined mouth facing down, first soak them in 0.1% potassium permanganate solution for 10 min to 15 min for disinfection, fish them out and rinse them with flowing clean water, and finally soak the disinfected cuttings with the inclined mouth facing down in the rooting agent solution, submerging the bottom 3 mm to 5 mm of the cuttings, and the soaking time is 5 h to 6 h.

[0012] Preferably, the rooting agent uses ABT rooting powder No. 1 powder produced by the Chinese Academy of Sciences. First, dissolve 1 g of rooting powder in 150 g of alcohol with a volume fraction of 75% to 100%, then add 10 kg to 15 kg of clear water, stir and pour it into the soaking pool. Each package of rooting agent can treat 4000 to 5000 cuttings.

[0013] Preferably, the cutting method in S5 is as follows: insert 1 cutting spike into each nutrient bag or plug tray, with the cutting depth being 5 - 7 cm, leaving 2 - 3 cm above, ensuring that 1 - 2 plump buds are exposed from the nutrient bag, compact the substrate to ensure close contact between the branch and the substrate, thoroughly water after cutting, and keep the soil moist.

[0014] Due to the adoption of the above technical solutions, the present invention has remarkable technical effects: The present invention provides a method for container mycorrhizal hardwood cutting seedling raising of leaf - used Lycium barbarum, using Glomus mosseae and Glomus intraradices as arbuscular mycorrhizal fungi for the propagation of Lycium barbarum seedlings, and mixing the mycorrhizal agent with clay to prepare a slurry for the hardwood cutting of Lycium barbarum. It is a new method combining mycorrhizal technology with traditional cutting, which can make the rooting rate of cuttings reach more than 95%, and significantly improve the survival rate (more than 10%), disease resistance and growth performance of Lycium barbarum cuttings. The present invention uses container mycorrhizal cultivation for leaf - used Lycium barbarum, which can shorten the seedling - raising cycle, improve the survival rate of seedlings, reduce the seedling - raising cost, and increase the income of farmers.

[0015] The following further elaborates the present invention with reference to embodiments. Specific implementation mode

[0016] Embodiment 1 This embodiment is a method for container mycorrhizal hardwood cutting seedling raising of leaf - used Lycium barbarum. The Lycium barbarum variety is Ningqi No. 9, and this method includes the following steps: S1. Selection of seedling - raising land Select a place close to the afforestation land for raising seedlings of Lycium barbarum containers. The annual average sunshine hours ≥ 2250 h, the annual average temperature ≥ 6.0 °C, the day - night temperature difference is 13 °C - 17 °C, the accumulated temperature ≥ 10 °C ≥ 1900 °C, there is a fresh - water source, and the salinity of the water ≤ 2 g / L.

[0017] S2. Preparation of mycorrhizal agent slurry S201. Selection of strains Select Glomus mosseae and Glomus intraradices as arbuscular mycorrhizal fungi for the propagation of Lycium barbarum seedlings.

[0018] S202. Multiplication of mycorrhizal agent In late May, Glomus mosseae was mixed evenly with pure river sand disinfected with 0.5% potassium permanganate solution at a mass ratio of 1:5 to make a 10 cm thick sand bed, and 8 corn seeds were planted per square meter of the sand bed; Glomus intraradices was mixed evenly with pure river sand disinfected with 0.5% potassium permanganate solution at a mass ratio of 1:5 to make a 10 cm thick sand bed, and 180 clover seeds were planted per square meter of the sand bed; after seedlings grew out in the two culture media, they were cultured with Hoagland nutrient solution. After 5 and a half months of inoculation, the aboveground parts of the host plants corn and clover were cut off, the river sand of the two fungal culture media were collected together, and the corn roots and clover roots were cut into 1cm to 2cm and mixed with the river sand and collected. This is the mycorrhizal agent for wolfberry hardwood cutting propagation.

[0019] S203, Mud Configuration The clay is dried, crushed and sieved, the mycorrhizal agent and clay are mixed into fungus soil at a mass ratio of 1:3, and then the obtained fungus soil and water are mixed evenly at a mass ratio of 1:5 to form a moderately viscous mud. The concentration of the mud should be suitable for evenly adhering to the surface of the branches. The mud concentration should not be too high to avoid affecting the air permeability of the branches. Among them, the clay is fine and free of impurities, and the particle size of the clay is ≤0.002mm, to ensure that the configured mud has strong adhesion, water absorption and air permeability, so that the mud configured by the mycorrhizal agent and clay can form a close contact with the wolfberry cuttings to the greatest extent, promoting rooting and bacterial infection.

[0020] S3. Cuttings processing S301, seed strip collection Select the healthy Ningqi No. 9 mother tree without diseases and insect pests. In early March, before the leaf wolfberry branches sprout, collect one-year-old lignified hard branches with a diameter of 0.5cm-0.8cm and a length of 50cm-100cm to obtain seed cuttings. The seed cuttings collected on the same day are stored in a completely shaded cold shed, sprayed with water and covered with plastic film to keep it moist. The temperature is controlled at 0℃-5℃, and the humidity is maintained at 70%-80%.

[0021] S302, cutting production Cuttings are made immediately after the seed strips are collected. Cut the seed strips into 8cm-10cm in length, retain 3-4 buds, cut the lower end of the cuttings into a "horseshoe" at a 45-degree angle, cut the upper end flat, and discard the part of the seed strip with a thickness of ≤0.4cm. Tie 50 cuttings into a bundle, with the oblique ends facing downward and the flat ends facing upward and sealed with red paint.

[0022] S303, cuttings processing For the bundled cuttings, first soak them in a 0.1% potassium permanganate solution for 10 minutes for disinfection, then take them out and rinse with running water to remove the residual potassium permanganate on the cuttings. Finally, place the disinfected cuttings with the inclined cut facing down neatly in the soaking pool and soak them in the solution prepared with rooting powder. The rooting agent uses ABT Rooting Powder No. 1 produced by the Chinese Academy of Sciences. Dissolve 1 g of rooting powder in 150 g of 75% (by volume) alcohol, add 10 kg of clear water, stir and pour it into the soaking pool. Each package of rooting agent can treat 4000 - 5000 cuttings. The water level in the container for soaking the cuttings should ensure that at least 3 mm of the bottom of the cuttings can be immersed in the rooting aqueous solution, and the soaking time is 5 hours.

[0023] S304. Sand storage for root induction In the warm greenhouse, place the treated cuttings neatly upside down on the ground with the inclined cut facing up, and cover them with 15 cm of wet sand (the humidity should be such that it forms a ball when held in the hand and loosens when released). After covering all the cuttings, control the temperature at 25°C, maintain the humidity of the sand, and induce roots for 7 days.

[0024] S4. Preparation of containers and substrates S401. Selection of containers Select non-woven fabric bottomless nutrient bags with a diameter ≥ 6 cm and a height ≥ 8 cm; S402. Selection of substrates The substrate is required to be loose, breathable, and have good water retention for the convenience of seedling root growth. The substrate ratio is: 65% coconut coir, 10% peat, 20% perlite, and 5% vermiculite. After mixing and stirring evenly, spray and disinfect with an 800-fold aqueous solution of 50% carbendazim to reduce germs, and then fill it into the nutrient bags for standby.

[0025] S5. Dipping in slurry and cutting S501. Preparation work before cutting Select a solar greenhouse, check whether the plastic film of the greenhouse is intact, replace the damaged greenhouse film, and add a layer of cold-proof straw curtain. Level the ground of the greenhouse, disinfect the soil, and install a sprinkler irrigation facility.

[0026] S502. Dipping in slurry treatment When obvious callus begins to appear at the inclined cut part of the cuttings, immerse the roots of the cuttings in the mycorrhizal agent slurry obtained in S2. Dip the bottom (inclined cut part) of the cuttings in the slurry for 3 cm - 5 cm, ensuring that the cut part is evenly covered with 2 mm - 3 mm of slurry, and then directly cut.

[0027] S503. Cutting operation Insert 1 cutting into each nutrient bag, with the cutting depth of 6 cm, and leave 2 cm - 3 cm above, ensuring that 1 - 2 plump buds are exposed outside the nutrient bag. Compact the substrate to ensure close contact between the branches and the substrate. After cutting, pour enough water to keep the soil moist.

[0028] S6. Management after Cuttage S601. Humidity Management After cuttage, water by micro-spraying. Water thoroughly for the first time, and then water when the soil is dry and wet as needed, spraying water in due time to keep the soil moist but avoid waterlogging. Especially during the rooting period (about 15d - 25d), water regularly. Spray 1 - 2 times a day before leaf emergence and root growth, and 4 - 6 times a day after leaf emergence and root growth. Generally, spray the first time at 9:00 - 10:00 in the morning on sunny days, each time for 30s - 50s, and spray once every 2h - 3h. Stop spraying after 17:00 in the afternoon. Keep the air humidity in the shed above 80% and the substrate humidity at 50% - 60%.

[0029] S602. Temperature Management After cuttage, close the ventilation openings, keep the environmental temperature around 20°C, and avoid direct sunlight and strong winds. Cover 80% of the shading net on the shed film, and only diffused light can be seen in the early stage. Control the temperature and light in the greenhouse with cotton quilts. Lift the cotton quilts at 9:00 in the morning to let the sunlight shine and warm up, and put down the cotton quilts after 16:00 in the afternoon to keep warm. Control the temperature at 22°C - 24°C during the day. Limited by the poor heat preservation of the greenhouse at night, it can only be maintained at about 7°C. After about 10d - 15d, the cuttings start to germinate. When the greenhouse temperature reaches 35°C, open the ventilation openings to cool down for 0.5h - 1h. When the young buds grow to about 3cm - 5cm, gradually increase the light transmittance and light exposure time. In about one week, the seedlings can grow to a height of 10cm. Observe the cuttings, and white new roots can be seen growing. When the rooting rate of the cuttings reaches more than 80%, the sunscreen net can be removed, and the temperature and light can be adjusted by using the ventilation openings and cotton quilts.

[0030] S603. Pest and Disease Control Check the seedlings regularly to prevent common pests and diseases of Lycium barbarum L. var. aurea Makino seedlings. Common pests of Lycium barbarum L. var. aurea Makino seedlings: Aceria palida Kerifer, Aphis sp., Paratioza sinica, lema decemounctata Gebler, etc. Use highly effective and low-toxic pesticides such as abamectin for foliar spraying; common diseases include: Lycium barbarum powdery mildew, Lycium barbarum root rot, etc. Use carbendazim, thiophanate-methyl, previcur, etc. to spray pesticides alternately for sterilization, and change to another fungicide every about one week to prevent diseases.

[0031] S604. Fertilization for Growth Promotion After about 4 weeks, when the young buds grow to about 10cm - 15cm, conduct foliar fertilization. Alternately spray a mixture of foliar nutrient solution and potassium dihydrogen phosphate every other day to promote root growth.

[0032] S605. Ventilation and Hardening-off After 30 to 40 days of cutting, the cuttings have basically taken root completely. Observe the nutrient bags, and it can be seen that the new roots have grown out of the non-woven fabric bags. At this time, the bottom air vents of the greenhouse can be opened about 5 to 10 cm, ventilated twice a day, and after 3 to 4 days, gradually increase the ventilation time and the width of the ventilation openings. Ventilate from the bottom for 10 days, then the upper ventilation openings can be opened to allow air convection until the branches and leaves of the seedlings are completely not afraid of sunlight and the ventilation size.

[0033] S606. Seedling pruning As the seedlings grow, when ventilation and light transmission are significantly poor, use a hedge trimmer to trim the upper branches to about 10 cm, and then immediately spray a fungicide to prevent the invasion of gray mold and root rot. Until about one week before the determined seedling emergence time, control the pruning height of the seedlings at about 20 cm from the ground. Similarly, spray a fungicide and start controlling water until the seedlings are out of the shed.

[0034] S607. Outplanting and loading Check the water content of the seedlings, keep the humidity appropriate, and outplant and load the seedlings.

[0035] Example 2 This example is a method for container mycorrhizal hardwood cutting seedling raising of leafy wolfberry. The wolfberry variety is Ningqicai No. 1. This method includes the following steps: S1. Selection of seedling raising land Select a place close to the afforestation land for raising wolfberry container seedlings. The annual average sunshine hours ≥ 2250 h, the annual average temperature ≥ 6.0 °C, the day-night temperature difference is 13 °C - 17 °C, the accumulated temperature of ≥ 10 °C activities ≥ 1900 °C, there is a fresh water source, and the salinity of the water ≤ 2 g / L.

[0036] S2. Preparation of mycorrhizal agent slurry S201. Selection of strains Select Glomus mosseae and Glomus intraradices as arbuscular mycorrhizal fungi for the breeding of wolfberry seedlings.

[0037] S202. Multiplication of mycorrhizal agent In late May, Glomus mosseae was mixed evenly with pure river sand disinfected with 0.5% potassium permanganate solution at a mass ratio of 1:8 to make a 10 cm thick sand bed, and 9 corn seeds were planted per square meter of the sand bed; Glomus intraradices was mixed evenly with pure river sand disinfected with 0.5% potassium permanganate solution at a mass ratio of 1:8 to make a 10 cm thick sand bed, and 190 clover seeds were planted per square meter of the sand bed; after seedlings grew out in the two culture media, they were cultured with Hoagland nutrient solution. After 5 and a half months of inoculation, the aboveground parts of the host plants corn and clover were cut off, the river sand of the two fungal culture media were collected together, and the corn roots and clover roots were cut into 1cm to 2cm and mixed with the river sand and collected. This is the mycorrhizal agent for wolfberry hardwood cutting propagation.

[0038] S203, Mud Configuration The clay is dried, crushed and sieved, the mycorrhizal agent and clay are mixed at a mass ratio of 1:4 to form a fungus soil, and then the obtained fungus soil and water are mixed at a mass ratio of 1:6 to form a moderately viscous mud, the concentration of which is suitable for evenly adhering to the surface of the branches. Among them, the clay is fine and free of impurities, and the particle size of the clay is ≤0.002mm.

[0039] S3. Cuttings processing S301, seed strip collection Select the healthy Ningqicai No. 1 mother tree without diseases and insect pests. In early March, before the leaf wolfberry branches sprout, collect one-year-old lignified hard branches with a diameter of 0.5cm-0.8cm and a length of 50cm-100cm to obtain seed cuttings. The seed cuttings collected on the same day are stored in a completely shaded fruit cellar, sprayed with water and covered with plastic film to keep it moist. The temperature is controlled at 0℃-5℃, and the humidity is maintained at 70%-80%.

[0040] S302, cutting production Cuttings are made immediately after the seed strips are collected. Cut the seed strips into 8cm-10cm in length, retain 3-4 buds, cut the lower end of the cuttings into a "horseshoe" at a 45-degree angle, cut the upper end flat, and discard the part of the seed strip with a thickness of ≤0.4cm. Tie 50 cuttings into a bundle, with the oblique ends facing downward and the flat ends facing upward and sealed with red paint.

[0041] S303, cuttings processing The bundled cuttings are first soaked in a 0.1% potassium permanganate solution for 12 minutes for disinfection, then fished out and rinsed with running water to remove the residual potassium permanganate on the cuttings. Finally, the disinfected cuttings are placed with the inclined mouth facing downwards neatly in the soaking pool and soaked in the solution prepared with rooting powder. The rooting agent uses ABT rooting powder No. 1 powder produced by the Chinese Academy of Sciences. 1 g of the rooting agent powder is fully dissolved in 150 g of alcohol with a volume fraction of 95%, diluted with 12 kg of clear water, stirred and then poured into the soaking pool. Each package of the rooting agent can treat 4,000 - 5,000 cuttings. The water level of the container for soaking the cuttings should ensure that at least 4 mm of the bottom of the cuttings can be soaked in the rooting aqueous solution, and the soaking time is 5.5 h.

[0042] S304. Sand storage for root induction In the greenhouse, the treated cuttings are neatly placed upside down on the ground with the inclined mouth facing upwards, covered with 10 cm of wet sand (the humidity is appropriate when it can be formed into a ball by hand and dispersed when released). After covering all the cuttings, the temperature is controlled at 22 °C, and the humidity of the sand is maintained to induce roots for 10 days.

[0043] S4. Preparation of containers and substrates S401. Selection of containers Select a 72-cell plastic plug tray as the container.

[0044] S402. Selection of substrates The substrate ratio is: 65% coconut coir, 10% peat, 20% perlite, 5% vermiculite. After mixing and stirring evenly, it is sprayed and disinfected with an 800-fold aqueous solution of 50% carbendazim and then filled into the plug trays for standby.

[0045] S5. Dipping in slurry and cutting S501. Preparation work before cutting Select a solar greenhouse, check whether the plastic film of the greenhouse is intact, replace the damaged greenhouse film, and add a cold-proof straw curtain. Level the greenhouse floor, disinfect the soil, and install a sprinkler irrigation facility.

[0046] S502. Dipping in slurry treatment When obvious callus begins to appear at the inclined mouth part of the cuttings, immerse the roots of the cuttings into the mycorrhizal agent slurry obtained in S2. The bottom of the cuttings (the inclined mouth part) is dipped in the slurry for 3 cm - 5 cm, ensuring that the cut part is evenly covered with the slurry for 2 mm - 3 mm, and then directly cut.

[0047] S503. Cutting operation Insert 1 cutting into each plug tray, the cutting depth is 5 cm, and 2 cm - 3 cm is reserved above, ensuring that 1 - 2 plump buds are exposed outside the nutrient bag. Compact the substrate to ensure that the branches are in close contact with the substrate. After cutting, water thoroughly and keep the soil moist.

[0048] S6. Management after cutting The processes such as temperature and humidity management, pest and disease control, promoting growth by foliar fertilization, ventilation and hardening off of seedlings, and pruning of seedlings are the same as those in Example 1, and finally the seedlings are lifted and loaded onto the truck.

[0049] Example 3 This example is a method for container mycorrhizal hardwood cutting seedling raising of leafy wolfberry. The wolfberry variety is Tianjing No. 5. This method includes the following steps: S1. Selection of seedling raising land For container seedling raising of wolfberry, select a place close to the afforestation land for seedling raising. The annual average sunshine hours ≥ 2250 h, the annual average temperature ≥ 6.0 °C, the diurnal temperature difference is 13 °C - 17 °C, the accumulated temperature ≥ 10 °C ≥ 1900 °C, there is a fresh water source, and the salinity of the water ≤ 2 g / L.

[0050] S2. Preparation of mycorrhizal agent slurry S201. Selection of strains Select Glomus mosseae and Glomus intraradices as arbuscular mycorrhizal fungi for wolfberry seedling breeding.

[0051] S202. Multiplication of mycorrhizal agent In late May, mix Glomus mosseae with pure river sand disinfected with 0.5% potassium permanganate solution evenly at a mass ratio of 1:10, make a 10 cm thick sand bed, and plant 10 corn seeds per square meter of the sand bed; mix Glomus intraradices with pure river sand disinfected with 0.5% potassium permanganate solution evenly at a mass ratio of 1:10, make a 10 cm thick sand bed, and plant 200 clover seeds per square meter of the sand bed; after seedlings grow in the two culture media, cultivate them with Hoagland nutrient solution. After 5.5 months of inoculation, cut off the above-ground parts of the host plants corn and clover, collect the river sand of the two fungal culture media together, cut the corn roots and clover roots into 1 cm - 2 cm and mix them evenly with the river sand, and collect them, which is the mycorrhizal agent for wolfberry hardwood cutting multiplication.

[0052] S203. Preparation of slurry Dry and crush the clay and sieve it. Mix the mycorrhizal agent and the clay at a mass ratio of 1:5 to form mycorrhizal soil, and then stir the obtained mycorrhizal soil and water evenly at a mass ratio of 1:7 to form a slurry with moderate viscosity. The concentration of the slurry should be such that it can evenly adhere to the surface of the branches. Among them, the clay selected is delicate and impurity-free clay, and the particle size of the clay ≤ 0.002 mm.

[0053] S3. Treatment of cuttings S301. Collection of cuttings Select healthy mother trees of Tianjing No. 5 without diseases and pests. In early March, before the buds of Lycium barbarum L. branches germinate, collect one-year-old lignified hard branches with a diameter of 0.5 cm to 0.8 cm and a length of 50 cm to 100 cm to obtain seed strips. The seed strips collected on the same day are stored in a completely shaded vegetable cellar, sprayed with water and covered with a plastic film for moisture preservation. The temperature is controlled at 0°C to 5°C, and the humidity is maintained at 70% to 80%.

[0054] S302. Cutting preparation Immediately make cuttings after collecting the seed strips. Cut the seed strips into lengths of 8 cm to 10 cm, retain 3 to 4 bud points. The lower end of the cutting is obliquely cut into a "horseshoe shape" at a 45-degree angle, and the upper end is cut flat. Discard the part of the seed strip with a thickness ≤ 0.4 cm. Bundle 50 cuttings into a bundle, with the oblique ends facing downwards evenly and the flat ends facing upwards, and seal the flat ends with red paint.

[0055] S303. Cutting treatment Soak the bundled cuttings first in a 0.1% potassium permanganate solution for 15 minutes for disinfection, then fish them out and rinse them with running water to remove the residual potassium permanganate on the cuttings. Finally, place the disinfected cuttings with the oblique ends facing downwards neatly in the soaking pool and soak them in the solution prepared with rooting powder. The rooting agent uses ABT rooting powder No. 1 powder produced by the Chinese Academy of Sciences. Dissolve 1 g of rooting agent powder in 150 g of alcohol (anhydrous ethanol) with a volume fraction of 100%, add 15 kg of clear water, stir and pour it into the soaking pool. Each package of rooting agent can treat 4000 to 5000 cuttings. The water level in the container for soaking the cuttings should ensure that at least 5 mm of the bottom of the cuttings can be soaked in the rooting aqueous solution, and the soaking time is 6 hours.

[0056] S304. Sand storage for root induction In the greenhouse, place the treated cuttings neatly upside down on the ground with the oblique ends facing upwards, and cover them with 20 cm of wet sand (the humidity is appropriate when it can be formed into a ball by hand and scattered when released). After covering all the cuttings, control the temperature at 30°C, keep the humidity of the sand, and induce roots for 5 days.

[0057] S4. Container and substrate preparation S401. Container selection Select a 50-cell plastic plug tray as the container.

[0058] S402. Substrate selection The substrate ratio is: 65% coconut coir, 10% peat, 20% perlite, 5% vermiculite. Mix and stir evenly, then spray and disinfect with an 800-fold aqueous solution of 50% carbendazim, and load it into the plug tray for later use.

[0059] S5. Dipping in slurry and cutting S501. Preparation work before cutting Select a solar greenhouse, check whether the greenhouse plastic film is intact, replace the damaged shed film, and lay additional cold-proof straw curtains. Level the greenhouse floor, disinfect the soil, and install sprinkler irrigation facilities.

[0060] S502, Slurry treatment When callus begins to appear significantly at the oblique cut part of the cutting, immerse the root of the cutting into the mycorrhizal agent slurry obtained in S2. The bottom (oblique cut part) of the cutting is smeared with slurry for 3 cm to 5 cm, ensuring that the cut part is evenly covered with slurry for 2 mm to 3 mm, and then directly cutt the cutting.

[0061] S503, Cutting operation Insert 1 cutting into each plug tray, with the cutting depth of 7 cm and 2 cm to 3 cm reserved above, ensuring that 1 to 2 plump buds are exposed outside the nutrient bag. Compact the substrate to ensure close contact between the branch and the substrate. After cutting, water thoroughly and keep the soil moist.

[0062] S6, Management after cutting Processes such as temperature and humidity management, pest and disease control, foliar fertilization for growth promotion, ventilation and hardening-off of seedlings, and pruning of seedlings are the same as those in Example 1, and finally the seedlings are lifted and loaded onto the vehicle.

[0063] The present invention provides a method for container mycorrhizal hardwood cutting seedling raising of Lycium barbarum L. for leaf use, which mixes a mycorrhizal agent with clay to prepare a slurry for hardwood cutting of Lycium barbarum L., and is an innovative method combining mycorrhizal technology with traditional cutting, which can significantly improve the rooting rate of cuttings and the survival rate, disease resistance and growth performance of Lycium barbarum L. cutting seedlings. Using container mycorrhizal cultivation for Lycium barbarum L. for leaf use can shorten the seedling raising period, improve the survival rate of seedlings, reduce the seedling raising cost, and increase the income of nursery farmers.

[0064] The above are only the preferred embodiments of the present invention, and do not impose any limitations on the present invention. Any simple modifications, changes, and equivalent variations made to the above embodiments according to the technical essence of the invention still fall within the protection scope of the technical solution of the present invention.

Claims

1. A method for raising leaf wolfberry seedlings by container mycorrhizal hardwood cuttings, characterized in that: The following steps are involved: S1. Selection of nursery site Choose a place close to the afforestation site as the wolfberry container seedling site; S2. Preparation of mycorrhizal agent slurry Inoculating Glomus mosseae and Glomus intraradices into corn and clover seeds respectively, and propagating the bacterial agent in a river sand matrix to obtain a mycorrhizal agent for wolfberry hardwood cuttings; mixing the mycorrhizal agent with clay, and adding water to obtain a mycorrhizal agent slurry; S3. Cuttings processing S301, seed cutting collection: select healthy, disease-free and insect-free leaf wolfberry mother trees, and collect seed cuttings in early March before the branches sprout; S302, cutting preparation: cutting the seed strips into a length of 8 cm to 10 cm, retaining 3 to 4 buds, cutting the lower end of the cuttings obliquely into a "horseshoe" at a 45-degree angle, cutting the upper end flat, and discarding the portion of the seed strip with a thickness of ≤0.4 cm to obtain cuttings; S303, cuttings treatment: the prepared cuttings are tied into bundles with the oblique mouth facing downwards, first soaked and disinfected in a 0.1% potassium permanganate solution, then rinsed with water, and then soaked in a rooting agent solution; S304, rooting in sand: place the treated cuttings on the ground with the slanting mouth facing upward, cover with 10-20 cm of wet sand, control the ambient temperature to 22-30°C, and root for 5-10 days; S4. Container and matrix preparation The container should be a nutrient bag or a plug tray. The matrix ratio is: 65% coconut bran, 10% peat, 20% perlite, and 5% vermiculite. After mixing well, spray with 50% carbendazim 800 times aqueous solution for disinfection, and put it into the nutrient bag or plug tray for later use. S5, Dip-slurry cutting When callus tissue begins to appear obviously at the oblique part of the cutting, immerse the root of the cutting into the mycorrhizal agent slurry obtained in S2, with the bottom of the cutting covered with slurry to a height of 3cm to 5cm, and the incision part covered with slurry to a thickness of 2mm to 3mm, and then directly plant the cutting; S6. Management after cutting After cuttings, temperature and humidity management should be carried out, pest and disease control should be carried out regularly, foliar fertilizer should be applied to promote growth, and ventilation, seedling hardening and seedling pruning should be carried out as appropriate.

2. The method according to claim 1, characterized in that The wolfberry container nursery land described in S1 has an annual average sunshine duration of ≥2250h, an annual average temperature of ≥6.0°C, a day-night temperature difference of 13°C to 17°C, an active accumulated temperature of ≥10°C, a fresh water source, and a water mineralization of ≤2g / L.

3. The method according to claim 1, characterized in that The method for propagating the mycorrhizal agent described in S2 is: in late May, Glomus mosseae is mixed evenly with pure river sand disinfected with 0.5% potassium permanganate solution at a mass ratio of 1:5 to 1:10 to make a 10 cm thick sand bed, and 8 to 10 corn seeds are planted per square meter of the sand bed; Glomus intraradices is mixed evenly with pure river sand disinfected with 0.5% potassium permanganate solution at a mass ratio of 1:5 to 1:10 to make a 10 cm thick sand bed, and 180 to 200 clover seeds are planted per square meter of the sand bed; after seedlings grow out in the two culture media, they are cultured with Hoagland nutrient solution, and after 5 and a half months of inoculation, the aboveground parts of the host plants corn and clover are cut off, the two fungal culture medium river sands are collected together, and the corn root system and the clover root system are cut into 1 cm to 2 cm and mixed with the river sand, and collected, which is the mycorrhizal agent for propagating wolfberry hardwood cuttings.

4. The method according to claim 1, characterized in that: The clay described in S2 is fine and impurity-free clay, and the particle size of the clay is ≤0.002mm; the preparation method of the mycorrhizal agent slurry is: the mycorrhizal agent and clay are mixed into mycorrhizal soil in a mass ratio of 1:3 to 1:5, and then the mycorrhizal soil and water are mixed into mycorrhizal agent slurry in a mass ratio of 1:5 to 1:

7.

5. The method according to claim 1, characterized in that The wolfberry varieties mentioned are all approved leaf-using wolfberry varieties.

6. The method according to claim 1, characterized in that The seed cutting collection in S301 is to collect one-year-old lignified hard branches with a diameter of 0.5 cm to 0.8 cm and a length of 50 cm to 100 cm to obtain seed cuttings.

7. The method according to claim 1, characterized in that The method for treating the cuttings described in S303 is: tie 50 prepared cuttings into a bundle with the oblique mouth facing downward, first soak them in 0.1% potassium permanganate solution for 10min to 15min for disinfection, then take them out and rinse them with running water, and finally soak the disinfected cuttings with the oblique mouth facing downward in a rooting agent solution, immersing the bottom of the cuttings by 3mm to 5mm, and the soaking time is 5h to 6h.

8. The method according to claim 7, characterized in that The rooting agent adopts ABT rooting powder No. 1 produced by the Chinese Academy of Sciences. 1g of rooting powder is first fully dissolved with 150g of alcohol with a volume fraction of 75% to 100%, then added with 10kg to 15kg of clean water, stirred and poured into a soaking tank. Each package of rooting agent can treat 4000 to 5000 cuttings.

9. The method according to claim 1, characterized in that: The cutting method described in S5 is: one cutting is cut into each nutrient bag or plug tray, the cutting depth is 5 to 7 cm, 2 cm to 3 cm is retained on the top, and 1 to 2 full buds are ensured to be exposed from the nutrient bag, the matrix is ​​compacted to ensure that the branches are in close contact with the matrix, and water is poured thoroughly after cutting to keep the soil moist.