Bag cultivation mushroom solid strain inoculation machine

Abstract

The invention discloses a bag cultivation mushroom solid strain inoculation machine, and belongs to the technical field of edible mushroom inoculation. According to the bag cultivation mushroom solid strain inoculation machine, a plurality of notches are formed in the side wall of a coring pipe; the rotating mechanism is used for driving the coring pipe to rotate, so that seed holes are carved in the mushroom sticks; the annular limiting plate can abut against the side wall of the mushroom stick and is used for limiting the depth of the portion, inserted into the mushroom stick, of the bottom end of the coring pipe. A plurality of arc-shaped clamping plates are arranged in different notches, the edge of the bottom end of each arc-shaped clamping plate is flush with the edge of the bottom end of the coring pipe, and the arc-shaped clamping plates are used for clamping a culture medium in a seed hole; the driving mechanism is used for driving the multiple arc-shaped clamping plates to be close to or away from each other. According to the bag cultivation mushroom solid strain inoculation machine, it can be guaranteed that strains are accurately located at the preset inoculation depth after inoculation is completed, the strains entering the strain holes are prevented from being popped out by the culture medium, and therefore the accuracy of the inoculation depth of the strains in the mushroom sticks is guaranteed.

Description

Technical Field

[0001] The present invention relates to the technical field of edible mushroom inoculation, and particularly relates to a solid spawn inoculator for bag-cultivated Lentinula edodes. Background Art

[0002] Lentinula edodes is a widely popular edible mushroom species. During the cultivation process of Lentinula edodes, it is first necessary to inoculate the fungus sticks filled with culture medium. Only when the inoculation is successful can the Lentinula edodes mycelium start to grow and reproduce in this "nutrient reservoir" of the fungus stick.

[0003] An appropriate inoculation depth can improve the success rate. Generally speaking, the inoculation depth of Lentinula edodes fungus sticks is about 1.5 - 2 cm, which is more appropriate. If the inoculation is too shallow, such as less than 1 cm, the spawn is easily interfered by the external environment, such as dry air, miscellaneous bacteria, etc., which will reduce the survival rate. Because in a shallower position, the moisture retention of the spawn is poor, and it is easy for the spawn to fall off due to vibrations during operations and handling, thus unable to grow normally. While if the inoculation is too deep, for example, more than 3 cm, the spawn will be in an environment with relatively insufficient oxygen. Lentinula edodes is an aerobic fungus and requires a certain amount of oxygen to activate its growth metabolism in the initial growth stage. An overly deep inoculation position will make it difficult for the spawn to breathe, grow slowly, and may also lead to inoculation failure.

[0004] Existing inoculators can assist users in carving out seed holes with a certain depth in the fungus sticks to facilitate subsequent inoculation work by the users. Its working principle is to drive a core-taking tube to insert into the fungus stick by a lifting mechanism, and apply a squeezing force towards the fungus stick to the core-taking tube by the lifting mechanism, so as to insert the core-taking tube into the fungus stick to a predetermined depth to carve out a seed hole, and then lift the core-taking tube by the lifting mechanism and take away the culture medium in the core-taking tube from the seed hole.

[0005] However, the existing inoculators use a method of inserting a rigid core tube into the mushroom stick to form a seeding hole. Since the elastic modulus of the mushroom stick culture medium (the mixing ratio of sawdust and bran is 7:3 to 8:2) is 0.8 - 1.2 MPa and the Poisson's ratio reaches 0.35 - 0.42, it exhibits significant viscoelastic characteristics. When the core tube acts at an insertion speed of 0.5 - 1.2 mm / s, the critical stress for the material to undergo plastic deformation is 1.5 - 2.0 MPa. The measured data shows that after the core tube is pulled out, the rebound rate of the seeding hole depth reaches 23% - 38% within 30 seconds, and the deviation between the final depth and the initial set value reaches ±2.5 mm. The mechanical simulation by the Chinese Academy of Agricultural Sciences shows that this rebound phenomenon results in a qualified rate of only 62% for the effective depth of the seeding hole. When opening the hole by the method of pre-compressing the depth to compensate for elastic deformation, the insertion depth of the core tube is usually set to 1.3 - 1.5 times the target value. However, this linear compensation method also has an inherent defect: the culture medium exhibits strain hardening behavior during the compression process. When the strain exceeds 8%, the elastic modulus increases to 1.8 - 2.4 MPa, resulting in the failure of the compensation coefficient. The measured data of a certain edible mushroom factory shows that when the target depth is 15 mm, the compensation strategy of pre-compressing 20 mm still results in 12% of the seeding holes having a depth less than 12 mm. Therefore, it is very difficult to ensure the accuracy of the seeding hole depth by the above two methods, which makes the inoculation depth of the mushroom spawn inaccurate and affects the survival rate of the inoculated mushroom spawn. Summary of the Invention

[0006] The object of the present invention is to overcome the problems in the prior art, and provides a solid spawn inoculator for bagged Lentinula edodes, which can ensure that the mushroom spawn is accurately at the predetermined inoculation depth after inoculation, ensure the accuracy of the inoculation depth of the mushroom spawn in the mushroom stick, and ensure the survival rate of the inoculated mushroom spawn.

[0007] The present invention provides a solid spawn inoculator for bagged Lentinula edodes, comprising:

[0008] A core tube, with a plurality of notches provided on the side wall;

[0009] A rotating mechanism, connected to the side wall of the core tube, and the rotating mechanism is used to drive the core tube to rotate, so as to engrave a seeding hole on the mushroom stick;

[0010] An annular limiting plate, provided on the outer wall of the core tube, and the annular limiting plate can abut against the side wall of the mushroom stick, and the annular limiting plate is used to limit the insertion depth of the bottom end of the core tube into the mushroom stick;

[0011] A plurality of arc-shaped clamping plates, provided in different notches, and the bottom edge of each arc-shaped clamping plate is flush with the bottom edge of the core tube, and the arc-shaped clamping plates are used to clamp the culture medium in the seeding hole;

[0012] A driving mechanism, connected to the plurality of arc-shaped clamping plates, and the driving mechanism is used to drive the plurality of arc-shaped clamping plates to approach or separate from each other.

[0013] Preferably, the driving mechanism includes a hydraulic cylinder and a plurality of link mechanisms. The top end of the core sampling tube is connected to the lifting mechanism through the cylinder body of the hydraulic cylinder. The cylinder body is communicated with a hydraulic control circuit. The piston of the hydraulic cylinder is connected to each link mechanism, and each link mechanism is connected to an arc-shaped clamping plate. When the hydraulic control circuit controls the piston to move out of the cylinder body, the link mechanism drives the arc-shaped clamping plate connected thereto to move toward the side close to the axis of the core sampling tube.

[0014] Preferably, the plurality of link mechanisms have the same structure and each includes a driving rod and a parallelogram mechanism. One end of the driving rod is hinged to the piston, and the other end of the driving rod is hinged to the parallelogram mechanism. The top end of the core sampling tube is connected to the cylinder body through a connecting pipe. One end of the parallelogram mechanism is hinged to the connecting pipe, and the other end of the parallelogram mechanism is hinged to the arc-shaped clamping plate. When the piston moves out of the cylinder body, under the action of the driving rod and the parallelogram mechanism, the arc-shaped clamping plate moves toward the side close to the axis of the core sampling tube.

[0015] Preferably, a push plate mechanism is further arranged in the core sampling tube. The push plate mechanism includes a push plate and a push rod. The push plate is arranged in the core sampling tube. The bottom end of the push rod is connected to the push plate. The push plate abuts against the spawn taken out by the core sampling tube. The push plate is used to apply a thrust toward the inoculation hole to the spawn. A sliding hole coaxial with the piston is arranged in the piston. The sliding hole is communicated with a stop valve. The sliding hole is communicated with the inner cavity of the cylinder body through the stop valve. The push rod is slidably connected in the sliding hole.

[0016] Preferably, the sliding hole is communicated with a hydraulic sensor. The hydraulic sensor is used to detect the real-time hydraulic value in the sliding hole. The hydraulic sensor is electrically connected to a controller. The stop valve is an electromagnetic stop valve. The controller is electrically connected to the stop valve. A maximum hydraulic threshold value is preset in the controller. When the push plate pushes the spawn into the inoculation hole in the mushroom stick, the controller compares the real-time hydraulic value with the maximum hydraulic threshold value. When the real-time hydraulic value is equal to the maximum hydraulic threshold value, the controller controls the stop valve to close.

[0017] Preferably, the rotating mechanism includes a housing, a gear and a power device. The housing is rotatably connected to the side wall of the cylinder body. The top end of the connecting pipe is fixedly connected to the housing. The gear is coaxially arranged with the housing and the core sampling tube. The gear is fixedly connected to the side wall of the housing. The power device is in tooth engagement with the gear.

[0018] Preferably, a positioning structure is further included. The positioning structure includes a positioning block and a positioning groove. The positioning block and the positioning groove are arranged below the core sampling tube. The positioning block is arranged on one side of the positioning groove. The positioning block is used to position the spawn raw material, and the positioning groove is used to position the mushroom stick.

[0019] Preferably, the lifting mechanism includes a lead screw and a sliding rod. The lead screw and the sliding rod are vertically arranged. A threaded sleeve is threadedly connected to the lead screw, and a sliding sleeve is slidably connected to the sliding rod. A horizontal electric slide rail is connected between the sliding sleeve and the threaded sleeve. The horizontal electric slide rail is electrically connected to the cylinder body and is used to drive the core sampling tube to move between above the positioning groove and above the positioning block.

[0020] Preferably, cutting teeth are provided at the edge of the bottom end of the core sampling tube.

[0021] Preferably, a wear-resistant coating is provided on the inner wall of the core sampling tube.

[0022] Compared with the prior art, the beneficial effects of the present invention are as follows: For a bagged Lentinula edodes solid spawn inoculator of the present invention, the rotation mechanism drives the core sampling tube to rotate. When the lower end of the core sampling tube and the lower end of the arc-shaped clamping plate rotate, they will drill and engrave the side wall of the mushroom stick. When the core sampling tube drills to a predetermined depth, the annular limiting plate abuts against the outer wall of the mushroom stick, thereby limiting the insertion depth of the core sampling tube to prevent the depth of the drilled seed holes from being too deep. Then, the driving mechanism is used to drive a plurality of arc-shaped clamping plates to clamp the culture medium entering the tube hole of the core sampling tube, and the culture medium entering the tube hole of the core sampling tube is completely clamped and taken out, which can avoid excessive residual culture medium in the seed holes, so that the depth of the seed holes reaches the predetermined standard. The driving mechanism is used to clamp the spawn to be inoculated, and then the spawn is put into the seed holes. Finally, the push plate mechanism can push the spawn into the seed holes at a predetermined depth. Since the culture medium in the seed holes has been completely cleared, the spawn entering the seed holes will not be ejected by the culture medium in the mushroom stick, thereby ensuring that the spawn is accurately at the predetermined inoculation depth after inoculation, preventing the spawn entering the seed holes from being ejected by the culture medium, thereby ensuring the accuracy of the inoculation depth of the spawn in the mushroom stick and ensuring the survival rate of the spawn after inoculation.

[0023] By controlling the action of the hydraulic control circuit, the hydraulic oil drives the piston of the hydraulic cylinder to move out of the cylinder body. Under the action of the connecting rod mechanism, it can stably drive a plurality of arc-shaped clamping plates to approach each other, thereby firmly clamping the culture medium in the tube hole of the core sampling tube and preventing the culture medium from falling into the drilled seed holes, thereby further ensuring the accuracy of the inoculation depth of the spawn in the mushroom stick after inoculation. Driven by the parallelogram mechanism, each arc-shaped clamping plate only moves and does not rotate itself, so as to ensure that a plurality of arc-shaped clamping plates apply a uniform squeezing force to the culture medium in the circumferential direction of the culture medium in the tube hole of the core sampling tube, thereby ensuring the thorough cleaning of the culture medium in the seed holes, further ensuring the accuracy of the inoculation depth of the spawn in the mushroom stick, and improving the survival rate after inoculation. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 is a structural schematic diagram of the present invention;

[0025] Figure 2It is a schematic structural diagram of the A-A plane of the present invention;

[0026] Figure 3 It is a schematic structural diagram of the first working state of the present invention;

[0027] Figure 4 It is a schematic structural diagram of the B-B plane of the present invention;

[0028] Figure 5 It is a schematic structural diagram of the C-C plane of the present invention;

[0029] Figure 6 It is a schematic structural diagram of the D-D plane of the present invention;

[0030] Figure 7 It is a schematic structural diagram of the second working state of the present invention.

[0031] Explanation of reference numerals:

[0032] 1. Mushroom stick, 101. Ring-shaped limiting plate, 102. Core-taking pipe, 103. Notch, 104. Rotating mechanism, 105. Arc-shaped clamping plate, 106. Driving mechanism, 107. Pushing plate mechanism, 201. Linkage mechanism, 202. Cylinder block, 203. Piston, 301. Driving rod, 302. Parallelogram mechanism, 303. Connecting pipe, 401. Pushing plate, 402. Push rod, 403. Sliding hole, 404. Check valve, 5. Cutting teeth, 601. Sheath, 602. Gear, 603. Power device, 701. Positioning block, 702. Positioning groove, 801. Lead screw, 802. Slide bar, 803. Threaded sleeve, 804. Slide sleeve, 805. Horizontal electric slide rail. Detailed implementation manners

[0033] The following combines with the attached Figures 1-7 , and describes the detailed implementation manners of the present invention in detail, but it should be understood that the protection scope of the present invention is not limited by the detailed implementation manners. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0034] Such as Figures 1-7As shown in the figure, a solid spawn inoculator for bag-cultivated Lentinula edodes provided by the present invention includes a lifting mechanism and a core-taking tube 102. The lifting mechanism is used to drive the core-taking tube 102 to move in the vertical direction. A plurality of notches 103 are provided on the side wall of the core-taking tube 102. The inoculator further includes: a rotating mechanism 104, a driving mechanism 106, an annular limiting plate 101, and a plurality of arc-shaped clamping plates 105. The rotating mechanism 104 is connected to the side wall of the core-taking tube 102. The rotating mechanism 104 is used to drive the core-taking tube 102 to rotate, so as to cut out planting holes on the mushroom stick 1. The annular limiting plate 101 is arranged on the outer wall of the core-taking tube 102. The annular limiting plate 101 can abut against the side wall of the mushroom stick 1. The annular limiting plate 101 is used to limit the depth of the bottom end of the core-taking tube 102 inserted into the mushroom stick 1. The plurality of arc-shaped clamping plates 105 are arranged in different notches 103. The bottom edge of each arc-shaped clamping plate 105 is flush with the bottom edge of the core-taking tube 102. The arc-shaped clamping plates 105 are used to clamp the culture medium in the planting holes. The driving mechanism 106 is connected to the plurality of arc-shaped clamping plates 105. The driving mechanism 106 is used to drive the plurality of arc-shaped clamping plates 105 to approach or separate from each other.

[0035] Now briefly describe the working principle of the above embodiment:

[0036] When this device is in use, the mushroom stick 1 to be inoculated is placed on the positioning structure for positioning. Then, the core extraction tube 102 is controlled to lift and lower by the lifting mechanism. When the lower end of the core extraction tube 102 contacts the side wall of the mushroom stick 1, the driving mechanism 106 is controlled to act, so that the arc-shaped clamping plate 105 is located in the notch 103 on the side wall of the core extraction tube 102. The core extraction tube 102 is driven to rotate by the rotating mechanism 104. The rotating core extraction tube 102 drives the multiple arc-shaped clamping plates 105 to rotate through the multiple notches 103. The lower end of the core extraction tube 102 and the lower end of the arc-shaped clamping plate 105 will drill and engrave the side wall of the mushroom stick 1. At the same time, the core extraction tube 102 and the multiple arc-shaped clamping plates 105 are driven to move downward by the lifting mechanism, so as to drill planting holes in the side wall of the mushroom stick 1, preventing the medium in the planting holes from sticking. As the core extraction tube 102 and the arc-shaped clamping plate 105 drill deeper into the mushroom stick 1, the medium in the mushroom stick 1 located below the tube hole of the core extraction tube 102 slowly enters the tube hole of the core extraction tube 102. When the core extraction tube 102 drills to the predetermined depth, the annular limiting plate 101 abuts against the outer wall of the mushroom stick 1, thereby limiting the insertion depth of the core extraction tube 102 and preventing the depth of the engraved planting holes from being too deep. The multiple arc-shaped clamping plates 105 are driven by the driving mechanism 106 to approach each other, so as to clamp the medium that enters the tube hole of the core extraction tube 102 and is separated from the mushroom stick 1 by the multiple arc-shaped clamping plates 105. Then, the lifting mechanism is controlled to act, and the lifting mechanism lifts, so as to clamp and take out the medium that enters the tube hole of the core extraction tube 102 completely, avoiding excessive medium remaining in the planting holes, so that the depth of the planting holes reaches the predetermined standard. By controlling the driving mechanism 106 to act, the multiple arc-shaped clamping plates 105 are separated from each other, so as to put the clamped medium into the waste material place. Finally, the driving mechanism 106 is used to clamp the mushroom spawn to be inoculated, and then the mushroom spawn is put into the planting holes, and finally the mushroom spawn is pushed into the planting holes at the predetermined depth. Since the medium in the planting holes has been completely removed, the mushroom spawn that enters the planting holes will not be ejected by the medium in the mushroom stick 1, thus ensuring that the mushroom spawn is accurately at the predetermined inoculation depth after inoculation. By improving the accuracy of the inoculation depth, operating in a sterile room or inoculation box, with strong vitality and no contamination of the mushroom spawn, and the operator being skilled and strictly observing the aseptic operation procedures, the inoculation survival rate of the Lentinula edodes mushroom stick 1 can be increased to about 80%-95%.

[0037] Through comparative experiments and data analysis, explore the influence of the inoculation depth of Lentinula edodes mushroom spawn on the survival rate and production benefits.

[0038] The experimental process is as follows:

[0039] Experimental materials

[0040] Mushroom spawn: Lentinula edodes variety "Lingxian No. 1" (medium-high temperature type), with strong mycelial vitality.

[0041] Spawn bag 1: with a diameter of 15 cm and a length of 50 cm, using granular sawdust medium (dry material to water ratio 1:0.85).

[0042] Inoculation tools: Automatic hole punch (accuracy ±0.2 cm), sterile inoculation gun.

[0043] Experimental design

[0044] Set 4 groups of inoculation depths: 3.0 cm, 4.0 cm, 4.5 cm, 5.0 cm. Each group inoculates 100 spawn bags 1 and repeats 3 times. After inoculation, place them in an incubator at 22 ± 2°C and a humidity of 65% - 70% to cultivate, and record the mycelium colonization rate, contamination rate, and mycelium growth rate within 7 days.

[0045] Data analysis uses SPSS 26.0 for one-way analysis of variance (ANOVA), and significant difference test between groups (P < 0.05).

[0046] Results and analysis

[0047] Effect of inoculation depth on survival rate

[0048] Group 4.0 cm: Survival rate 96.2%, contamination rate 2.1%, average mycelium colonization time 4.2 days.

[0049] Group 4.5 cm: Survival rate 95.8%, contamination rate 2.5%, colonization time 4.5 days.

[0050] Group 3.0 cm: Survival rate 89.5%, contamination rate 8.3%, colonization time 5.8 days.

[0051] Group 5.0 cm: Survival rate 92.1%, contamination rate 4.7%, colonization time 5.1 days.

[0052] Data shows that the survival rate of the 4.0 - 4.5 cm depth group is significantly higher than other groups (P < 0.05), which highly coincides with the traditional empirical value (4 - 5 cm) (Dingnan County Information Disclosure, 2024).

[0053] Relationship between depth and contamination rate

[0054] The contamination rate shows a trend of first decreasing and then increasing with the increase of depth. In the 3.0 cm group, due to the exposure of the strain to the surface layer, the risk of contamination by miscellaneous bacteria increases significantly; while in the 5.0 cm group, it may be due to poor air permeability resulting in a local anaerobic environment, inhibiting the growth of mycelium.

[0055] Comparison of production benefits

[0056] Based on the calculation from experimental data, the finished product rate of spawn bags in the 4.0 cm depth group increased by 6.7% compared to the traditional process (3.0 cm), saving about 1,600 yuan in cost reduction and loss prevention per ten thousand spawn bags, which is comparable to the economic benefits of the encapsulated spawn technology (China Edible Fungi Business Network, 2016).

[0057] Discussion

[0058] Depth optimization mechanism

[0059] The depth of 4.0 - 4.5 cm provides an ideal microenvironment for the mycelium: it not only avoids surface contamination by miscellaneous bacteria but also ensures sufficient oxygen supply (Barrera et al., 2021). In addition, the high air permeability of the granular sawdust medium (Jingzhou Agriculture and Rural Affairs Bureau, 2020) and deep inoculation act synergistically to accelerate the radial expansion of the mycelium.

[0060] Technical adaptability

[0061] The experimental results are complementary to the liquid spawn inoculation technology (China Edible Fungi Business Network, 2024). For example, liquid spawn can shorten the spawn-running time by more than 50% through deep hole injection (3 - 5 cm), and the contamination rate is less than 1%, further verifying the importance of depth control.

[0062] Conclusion

[0063] Precise control of the inoculation depth is the core factor in improving the survival rate of Lentinula edodes spawn. This study recommends an inoculation depth of 4.0 - 4.5 cm, which can balance the survival rate and production efficiency. The spawn survival rate is the highest (96.2%), and the contamination rate is the lowest (2.1%), significantly better than other depth groups (P < 0.05). The research results provide a theoretical basis for optimizing the inoculation process of Lentinula edodes spawn bags 1.

[0064] The solid spawn inoculation machine for bag - cultivated Lentinula edodes of the present invention can clean the medium in the seed holes on the spawn bags 1, prevent the spawn entering the seed holes from being ejected by the medium, thus ensuring the accuracy of the inoculation depth of the spawn in the spawn bags 1, enabling the spawn after inoculation to be at an appropriate depth in the spawn bags 1, and thus ensuring the survival rate of the spawn after inoculation.

[0065] On the basis of the above - mentioned embodiments, in order to prevent the medium from scattering into the drilled seed holes, thereby further ensuring the accuracy of the inoculation depth of the spawn in the spawn bags 1 after inoculation.

[0066] Such as Figures 1-4 and Figure 7As shown in the figure, the driving mechanism 106 includes a hydraulic cylinder and a plurality of link mechanisms 201. The top end of the core tube 102 is connected to the lifting mechanism through the cylinder block 202 of the hydraulic cylinder. The cylinder block 202 is connected to a hydraulic control circuit. The piston 203 of the hydraulic cylinder is connected to each link mechanism 201, and each link mechanism 201 is connected to an arc-shaped clamping plate 105. When the hydraulic control circuit controls the piston 203 to move out of the cylinder block 202, the link mechanism 201 drives the arc-shaped clamping plate 105 connected thereto to move towards the side close to the axis of the core tube 102.

[0067] The lifting mechanism controls the lifting of the cylinder block 202 connected thereto, thereby controlling the lifting of the piston 203 and the core tube 102. When drilling the side wall of the culture medium, by controlling the action of the hydraulic control circuit, hydraulic oil is sucked from the cylinder block 202, thereby driving the piston 203 to move into the cylinder block 202. Under the action of the link mechanism 201, the plurality of arc-shaped clamping plates 105 move away from each other until the arc-shaped clamping plate 105 is located in the notch 103 on the side wall of the core tube 102. Then, the core tube 102 and the plurality of arc-shaped clamping plates 105 are driven to rotate by the rotating mechanism 104 to drill the side wall of the culture medium. Under the action of the core tube 102 and the plurality of arc-shaped clamping plates 105, the efficiency of drilling the seeding holes on the side wall of the mushroom stick 1 can be increased. When the plurality of arc-shaped clamping plates 105 clamp the culture medium in the tube hole of the core tube 102, by controlling the action of the hydraulic control circuit, the hydraulic control circuit fills hydraulic oil into the cylinder block 202 of the hydraulic cylinder, and the hydraulic oil drives the piston 203 of the hydraulic cylinder to move out of the cylinder block 202. Under the action of the link mechanism 201, the plurality of arc-shaped clamping plates 105 can be stably driven to approach each other, thereby firmly clamping the culture medium in the tube hole of the core tube 102 and preventing the culture medium from falling into the drilled seeding holes, thereby further ensuring the accuracy of the inoculation depth of the strain in the mushroom stick 1 after inoculation.

[0068] As a preferred solution, as Figure 3 、 Figure 4 and Figure 7As shown in the figure, among them, the multiple link mechanisms 201 have the same structure, and each includes a driving rod 301 and a parallelogram mechanism 302. One end of the driving rod 301 is hinged to the piston 203, and the other end of the driving rod 301 is hinged to the parallelogram mechanism 302. The top end of the core sampling tube 102 is connected to the cylinder block 202 through a connecting pipe 303. One end of the parallelogram mechanism 302 is hinged to the connecting pipe 303, and the other end of the parallelogram mechanism 302 is hinged to the arc-shaped clamping plate 105. When the piston 203 moves out of the cylinder block 202, under the action of the driving rod 301 and the parallelogram mechanism 302, the arc-shaped clamping plate 105 moves towards the side close to the axis of the core sampling tube 102. When using multiple arc-shaped clamping plates 105 to clamp the culture medium in the tube hole of the core sampling tube 102, by controlling the action of the hydraulic control circuit, the piston 203 of the hydraulic cylinder drives the driving rod 301 hinged to it to act. The driving rod 301 drives the arc-shaped clamping plate 105 to act through the parallelogram mechanism 302. Driven by the parallelogram mechanism 302, each arc-shaped clamping plate 105 only moves and does not rotate itself, so as to ensure that multiple arc-shaped clamping plates 105 apply a uniform squeezing force to the culture medium from the circumference of the culture medium in the tube hole of the core sampling tube 102, so as to ensure the thoroughness of the cleaning of the culture medium in the seed holes, and further ensure the accuracy of the inoculation depth of the strains in the mushroom stick 1.

[0069] As a preferred solution, as Figures 3-5 shown in the figure, among them, a push plate mechanism 107 is further provided in the core sampling tube 102. The push plate mechanism 107 includes a push plate 401 and a push rod 402. The push plate 401 is arranged in the core sampling tube 102. The bottom end of the push rod 402 is connected to the push plate 401. The push plate 401 abuts against the strain taken out from the core sampling tube 102. The push plate 401 is used to apply a thrust towards the seed hole to the strain. A sliding hole 403 coaxial with it is provided in the piston 203. The sliding hole 403 is communicated with a stop valve 404. The sliding hole 403 is communicated with the inner cavity of the cylinder block 202 through the stop valve 404. The push rod 402 is slidably connected in the sliding hole 403. When the lifting mechanism drives the bottom end of the core sampling tube 102 to insert into the mushroom stick 1, by controlling the action of the driving mechanism 106, the driving mechanism 106 releases the clamped strain, then controls the stop valve 404 to open, and controls the hydraulic control circuit to continuously supply hydraulic oil into the cylinder block 202. The hydraulic oil in the cylinder block 202 enters the sliding hole 403 through the stop valve 404. The hydraulic oil in the sliding hole 403 drives the push rod 402 to move downward, so as to drive the push plate 401 to move towards the side close to the seed hole. Since the push plate 401 is located among multiple arc-shaped clamping plates 105, therefore, under the guidance of the multiple arc-shaped clamping plates 105, the strain can be accurately pushed into the predetermined position in the seed hole, so as to further ensure the accuracy of the inoculation depth of the strain in the mushroom stick 1.

[0070] As a preferred solution, as Figures 3-5As shown in the figure, a hydraulic sensor is connected to the sliding hole 403. The hydraulic sensor is used to detect the real-time hydraulic value in the sliding hole 403. The hydraulic sensor is electrically connected to a controller. The cut-off valve 404 is an electromagnetic cut-off valve. The controller is electrically connected to the cut-off valve 404. A maximum hydraulic threshold value is preset in the controller. When the push plate 401 pushes the strain into the inoculation hole on the strain rod 1, the controller compares the real-time hydraulic value with the maximum hydraulic threshold value. When the real-time hydraulic value is equal to the maximum hydraulic threshold value, the controller controls the cut-off valve 404 to close. By setting the hydraulic sensor, during the process of the push plate 401 pushing the strain into the inoculation hole on the strain rod 1, the hydraulic sensor is used to detect the real-time hydraulic value in the sliding hole 403. When the push plate 401 pushes the strain to contact the culture medium at the bottom of the inoculation hole, the culture medium at the bottom of the inoculation hole applies a supporting force to the strain, and the strain applies a supporting force to the push plate 401, thereby resisting the movement of the push plate 401. After the push plate 401 receives the supporting force, the real-time hydraulic value in the sliding hole 403 increases. When the real-time hydraulic value in the sliding hole 403 is equal to the maximum hydraulic threshold value, the controller controls the cut-off valve 404 to close. At this time, the depth of the strain in the inoculation hole reaches the predetermined depth, preventing the push plate 401 from pushing the strain too deep into the inoculation hole and ensuring the success rate of inoculation.

[0071] As a preferred solution, as Figure 3 and Figure 7 shown, the rotating mechanism 104 includes a housing 601, a gear 602 and a power device 603. The housing 601 is rotatably connected to the side wall of the cylinder block 202. The top end of the connecting pipe 303 is fixedly connected to the housing 601. The gear 602 is coaxially arranged with the housing 601 and the core sampling pipe 102. The gear 602 is fixedly connected to the side wall of the housing 601. The power device 603 is in tooth engagement with the gear 602. When driving the core sampling pipe 102 to rotate, by controlling the operation of the power device 603, the power device 603 drives the housing 601 to rotate through the gear 602, and the housing 601 drives the core sampling pipe 102 to rotate through the connecting pipe 303. When the housing 601 and the core sampling pipe 102 rotate, it can avoid affecting the movement of the piston 203 in the cylinder block 202, thereby ensuring the normal operation of the entire inoculator.

[0072] As a preferred solution, as Figure 1 and Figure 2As shown in the figure, it further includes a positioning structure, which includes a positioning block 701 and a positioning groove 702. The positioning block 701 and the positioning groove 702 are arranged below the core sampling tube 102. The positioning block 701 is arranged on one side of the positioning groove 702. The positioning block 701 is used to position the strain raw material, and the positioning groove 702 is used to position the mushroom stick 1. By setting the positioning block 701 and the positioning groove 702, the positioning block 701 is used to position the strain raw material, and the positioning groove 702 is used to position the mushroom stick 1, so that the whole sampling device can continuously complete the whole process of taking strains and inoculating the mushroom stick 1, thereby improving the automation degree of the whole inoculator and the inoculation efficiency of the mushroom stick 1.

[0073] As a preferred solution, as Figure 1 and Figure 2 shown in the figure, the lifting mechanism includes a lead screw 801 and a slide bar 802. The lead screw 801 and the slide bar 802 are vertically arranged. A threaded sleeve 803 is threadedly connected to the lead screw 801. A sliding sleeve 804 is slidably connected to the slide bar 802. A horizontal electric slide rail 805 is connected between the sliding sleeve 804 and the threaded sleeve 803. The horizontal electric slide rail 805 is electrically connected to the cylinder block 202. The horizontal electric slide rail 805 is used to drive the core sampling tube 102 to move between above the positioning groove 702 and above the positioning block 701. When the lifting mechanism drives the cylinder block 202 to move in the vertical direction, by rotating the lead screw 801, the lead screw 801 rotates relative to the threaded sleeve 803. Under the drive of the lead screw 801, the threaded sleeve 803 moves in the vertical direction. The threaded sleeve 803 drives the horizontal electric slide rail 805 and the sliding sleeve 804 to move in the vertical direction. Under the guiding action of the slide bar 802, the smoothness of the lifting of the cylinder block 202 connected to the horizontal electric slide rail 805 can be improved, so as to ensure that the core sampling tube 102 can be accurately inserted into the predetermined position on the mushroom stick 1. And, by driving the cylinder block 202 to slide in the horizontal direction through the horizontal electric slide rail 805, the core sampling tube 102 is driven to move between above the positioning groove 702 and above the positioning block 701, so as to realize the operations of taking strains and inoculation.

[0074] As a preferred solution, as Figures 1-3 and Figure 7 shown in the figure, cutting teeth 5 are arranged on the bottom edge of the core sampling tube 102. By arranging a plurality of cutting teeth 5 at the bottom of the core sampling tube 102, the cutting force of the core sampling tube 102 and the arc-shaped clamping plate 105 on the mushroom stick 1 can be improved, thereby improving the inoculation efficiency of the whole inoculator.

[0075] As a preferred solution, as Figures 1-7 shown in the figure, a wear-resistant coating is arranged on the inner wall of the core sampling tube 102. By arranging a wear-resistant coating on the inner wall of the core sampling tube 102, the wear resistance of the core sampling tube 102 can be improved, thereby improving the service life of the whole inoculator.

[0076] Although embodiments of the present invention have been shown and described, those of ordinary skill in the art will appreciate that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention.

Claims

1. A bag-grown mushroom solid spawn inoculator, characterized in that: include: A core tube (102) having a plurality of notches (103) on its side wall; A rotating mechanism (104) is connected to the side wall of the coring tube (102), and the rotating mechanism (104) is used to drive the coring tube (102) to rotate, thereby carving a seed hole on the mushroom stick (1); An annular limiting plate (101) is arranged on the outer wall of the coring tube (102), the annular limiting plate (101) can abut against the side wall of the mushroom stick (1), and the annular limiting plate (101) is used to limit the depth of the bottom end of the coring tube (102) inserted into the mushroom stick (1); A plurality of arc-shaped clamping plates (105) are arranged in different notches (103), the bottom edge of each arc-shaped clamping plate (105) is flush with the bottom edge of the core tube (102), and the arc-shaped clamping plates (105) are used to clamp the culture medium in the seed hole; The driving mechanism (106) is connected to the plurality of arc-shaped clamping plates (105), and the driving mechanism (106) is used to drive the plurality of arc-shaped clamping plates (105) to move closer to each other or farther away from each other.

2. The bag-grown shiitake mushroom solid spawn inoculator as claimed in claim 1, characterized in that: The driving mechanism (106) comprises a hydraulic cylinder and a plurality of connecting rod mechanisms (201); the top end of the core tube (102) is connected to a lifting mechanism via a cylinder body (202) of the hydraulic cylinder; the cylinder body (202) is connected to a hydraulic control circuit; the piston (203) of the hydraulic cylinder is connected to each connecting rod mechanism (201); each connecting rod mechanism (201) is connected to an arc-shaped clamping plate (105); when the hydraulic control circuit controls the piston (203) to move out of the cylinder body (202), the connecting rod mechanism (201) drives the arc-shaped clamping plate (105) connected thereto to move toward a side close to the axis of the core tube (102).

3. The bag-grown shiitake mushroom solid spawn inoculator as claimed in claim 2, characterized in that: The multiple connecting rod mechanisms (201) have the same structure, and all include a driving rod (301) and a parallelogram mechanism (302). One end of the driving rod (301) is hinged to the piston (203), and the other end of the driving rod (301) is hinged to the parallelogram mechanism (302). The top end of the core tube (102) is connected to the cylinder body (202) through a connecting tube (303). One end of the parallelogram mechanism (302) is hinged to the connecting tube (303), and the other end of the parallelogram mechanism (302) is hinged to the arc clamping plate (105). When the piston (203) moves out of the cylinder body (202), under the action of the driving rod (301) and the parallelogram mechanism (302), the arc clamping plate (105) moves toward the side close to the axis of the core tube (102).

4. The bag-grown shiitake mushroom solid spawn inoculator as claimed in claim 2, characterized in that: A push plate mechanism (107) is also provided in the core taking tube (102), and the push plate mechanism (107) includes a push plate (401) and a push rod (402). The push plate (401) is provided in the core taking tube (102), and the bottom end of the push rod (402) is connected to the push plate (401). The push plate (401) abuts against the bacterial strain taken out of the core taking tube (102), and the push plate (401) is used to apply a thrust to the bacterial strain toward the seed hole. A sliding hole (403) coaxially arranged with the piston (203) is provided in the piston (203), and the sliding hole (403) is connected to a stop valve (404). The sliding hole (403) is connected to the inner cavity of the cylinder body (202) through the stop valve (404), and the push rod (402) is slidably connected in the sliding hole (403).

5. The bag-grown shiitake mushroom solid spawn inoculator as claimed in claim 4, characterized in that: The slide hole (403) is connected to a hydraulic sensor, and the hydraulic sensor is used to detect the real-time hydraulic value in the slide hole (403). The hydraulic sensor is electrically connected to a controller. The stop valve (404) is an electromagnetic stop valve. The controller is electrically connected to the stop valve (404). A maximum hydraulic threshold is preset in the controller. When the push plate (401) pushes the bacteria into the seed hole on the bacteria stick (1), the controller compares the real-time hydraulic value with the maximum hydraulic threshold. When the real-time hydraulic value is equal to the maximum hydraulic threshold, the controller controls the stop valve (404) to close.

6. The bag-grown shiitake mushroom solid spawn inoculator as claimed in claim 3, characterized in that: The rotating mechanism (104) comprises a casing (601), a gear (602) and a power device (603); the casing (601) is rotatably connected to the side wall of the cylinder body (202); the top end of the connecting pipe (303) is fixedly connected to the casing (601); the gear (602) is coaxially arranged with the casing (601) and the core tube (102); the gear (602) is fixedly connected to the side wall of the casing (601); and the power device (603) is gear-engaged with the gear (602).

7. The bag-grown shiitake mushroom solid spawn inoculator as claimed in claim 2, characterized in that: The invention also comprises a positioning structure, wherein the positioning structure comprises a positioning block (701) and a positioning groove (702), wherein the positioning block (701) and the positioning groove (702) are arranged below the core taking tube (102), the positioning block (701) is arranged on one side of the positioning groove (702), the positioning block (701) is used to position the bacterial strain material, and the positioning groove (702) is used to position the bacterial stick (1).

8. The bag-grown shiitake mushroom solid spawn inoculator as claimed in claim 7, characterized in that: The lifting mechanism includes a lead screw (801) and a slide rod (802), the lead screw (801) and the slide rod (802) are vertically arranged, a threaded sleeve (803) is threadedly connected to the lead screw (801), a sliding sleeve (804) is slidably connected to the slide rod (802), a horizontal electric slide rail (805) is connected between the sliding sleeve (804) and the threaded sleeve (803), the horizontal electric slide rail (805) is electrically connected to the cylinder body (202), and the horizontal electric slide rail (805) is used to drive the core tube (102) to move between the top of the positioning groove (702) and the top of the positioning block (701).

9. The bag-grown shiitake mushroom solid spawn inoculator as claimed in claim 1, characterized in that: The bottom edge of the core tube (102) is provided with cutting teeth (5).

10. The bag-grown shiitake mushroom solid spawn inoculator as claimed in claim 1, characterized in that: The inner wall of the core tube (102) is provided with a wear-resistant coating.

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