Insecticidal composition containing dinotefuran

Abstract

The invention relates to the technical field of pesticides, and discloses an insecticidal composition containing dinotefuran. The insecticidal composition contains 9-51% of effective active components, 1.5-15% of a synergist, 5-40% of an auxiliary agent and the balance of water, the effective active components are dinotefuran and fenobucarb, and the weight ratio of dinotefuran to fenobucarb is 1: 2. The synergist is a mixture of a biological enzyme and a polymer emulsifier, wherein the weight ratio of the biological enzyme to the polymer emulsifier is 7: 1-1: 1. The insecticidal composition disclosed by the invention is used for preventing and treating rice planthoppers, can obviously improve the permeability and wettability of a medicament, can act on pests from multiple aspects, achieves the purpose of effectively preventing and treating the pests, reduces the dosage of the medicament in the field, saves the economic cost, reduces the pollution of the medicament to the environment, and has good economic benefits and economic benefits. And the risk that pests generate resistance to the pesticide is also reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and particularly relates to an insecticidal composition containing dinotefuran and application thereof. Background Art

[0002] Dinotefuran is a third-generation nicotinoid insecticide that acts on the insect neurotransmitter system and has contact and stomach poisoning effects. It can be quickly absorbed by plants and widely distributed in crops. Dinotefuran is a stimulant of nicotinic acetylcholine receptors and can affect the synapses of the insect central nervous system.

[0003] Fenbuterol is a carbamate insecticide with strong contact killing effect, and has certain stomach poison, fumigation and ovicidal effects. It mainly causes pests to die by poisoning by inhibiting acetylcholinesterase.

[0004] Rice leafhoppers have piercing-sucking mouthparts, which suck the juice of rice through their mouthparts, thereby interfering with the normal arrangement of photosynthetic products of the plant, reducing the nutrients transported to the root system, thereby disrupting the normal physiological activities of the root system, consuming the nutrients stored in the plant, resulting in insufficient nutrient accumulation, unfull grains, and increased barren grain rate. While sucking nutrients from rice, rice leafhoppers also carry pathogens such as viral diseases, grassy bush dwarf disease, and toothed leaf dwarf disease. Rice sheath blight and micrococcal sclerotinia infect crops through the large number of wounds caused by leafhoppers. Because rice leafhoppers reproduce quickly and reproduce all year round with overlapping generations, farmers use a large amount of pesticides to prevent and control pests in rice fields. Various types of pesticides are mixed together, causing the natural enemies of rice leafhoppers to disappear in large numbers, so the number of rice leafhoppers is increasing. In addition, the excessive use of pesticides has caused rice leafhoppers to develop resistance to pesticides. The living environment chosen by rice planthoppers is generally very hidden, and their distribution areas are different, so it is difficult to spray all the rice when applying pesticides, so it is impossible to effectively prevent and control rice planthoppers.

[0005] How to solve the problem of rice planthopper resistance, improve the permeability and wettability of pesticides, increase the utilization rate of pesticides, and reduce the amount of pesticides used are issues that need to be urgently addressed. Summary of the invention

[0006] The purpose of the present invention is to provide an insecticide composition containing dinotefuran, which can effectively control rice planthoppers. The insecticide composition of the present invention can significantly increase the permeability and wettability of the agent, can act on pests from multiple aspects, and achieve the purpose of effectively controlling the pests. At the same time, the dosage of the agent used in the field is reduced, the economic cost is saved, the pollution of the agent to the environment is reduced, and the risk of the pests developing resistance to the agent is also reduced.

[0007] The technical solution of the present invention is:

[0008] An insecticidal composition containing dinotefuran, characterized in that: the insecticidal composition contains 9-51% of active ingredients, 1.5-15% of synergists, 5-40% of auxiliaries, and the balance is made up to 100% with water. The active ingredients are dinotefuran and fenobucarb, and the weight ratio of dinotefuran to fenobucarb is 1:2. The synergist is a mixture of bioenzyme and high molecular emulsifier, and the weight ratio of bioenzyme to high molecular emulsifier is 7:1 to 1:1, optionally 5:1 to 1:1, and preferably 3:1 to 1:1.

[0009] Furthermore, the bioenzyme is selected from one of glucoamylase, serrapeptase, and keratinase; the high molecular emulsifier is selected from one of dodecylphenol polyoxyethylene ether, polyoxyethylene polyoxypropylene block copolymer, polyisobutylene anhydride, and fatty alcohol polyoxyethylene ether.

[0010] Furthermore, the auxiliary contains one or more of solvent, antifreeze, and defoamer.

[0011] Furthermore, the solvent is selected from one or more of solvent oil, cyclohexanone, dimethyl sulfoxide, N,N-dimethylformamide, sec-butyl acetate, N-methylpyrrolidone, methyl oleate, methanol, butanol, polyethylene glycol, and vegetable oil;

[0012] The antifreeze is selected from one or more of propylene glycol, glycerol, ethylene glycol, and urea;

[0013] The defoamer is selected from one or more of silicone ketone, fatty alcohol, and fatty acid.

[0014] Furthermore, the insecticidal composition contains: 3-17% of dinotefuran, 6-34% of fenobucarb, 2-10% of synergist, 6-50% of auxiliaries, and the balance is water; optionally, the insecticidal composition contains: 5-12% of dinotefuran, 10-24% of fenobucarb, 2-7% of synergist, 5-40% of solvent, 1-7% of antifreeze, 0.05-1% of defoamer, and the balance is water; optionally, the insecticidal composition contains: 10% of dinotefuran, 20% of fenobucarb, 5% of synergist, 10-30% of solvent, 2-6% of antifreeze, 0.05-0.5% of defoamer, and the balance is water.

[0015] Furthermore, it is characterized in that: the application of the insecticidal composition in controlling rice planthoppers.

[0016] Furthermore, when the insecticidal composition is used to control rice planthoppers, it is applied at the early peak stage of the low-instar nymphs of rice planthoppers, and is sprayed once at a dosage of 100-300 g / ha of the active ingredient, optionally, the dosage of the active ingredient is 130-250 g / ha.

[0017] Compared with the prior art, the composition of the present invention has the following beneficial effects: (1) Compared with single agents, the composition has obvious synergistic effects on rice planthoppers, increases the permeability and wettability of the agents, and significantly improves the control effect; (2) Reduces the amount of pesticides used, saves economic costs, and reduces the pollution of the agents to the environment; (3) Reduces the dosage of the agents in the field and also reduces the risk of pests developing resistance to the agents; (4) Improves the disease and pest resistance ability of crops, enhances the immunity of crops, and enhances photosynthesis. Detailed implementation methods

[0018] The present invention will be further described below in conjunction with embodiments, but the present invention is not limited thereto.

[0019] Application Example 1 The pesticidal compositions disclosed in the following examples contain the following components in percentage by content:

[0020] Example 1 30% dinotefuran·fenobucarb microemulsion

[0021] 10% dinotefuran technical, 20% fenobucarb technical, 5% glucoamylase, 2% fatty alcohol polyoxyethylene ether, 12% methyl oleate, 8% N-methylpyrrolidone, 6% glycerol, 0.1% fatty alcohol, and the balance is water, to obtain 30% dinotefuran·fenobucarb microemulsion.

[0022] Example 2 30% dinotefuran·fenobucarb microemulsion

[0023] 10% dinotefuran technical, 20% fenobucarb technical, 3% serrapeptase, 2.5% polyisobutenyl succinic anhydride, 8% N,N-dimethylformamide, 12% cyclohexanone, 6% ethylene glycol, 0.05% fatty alcohol, and the balance is water, to obtain 30% dinotefuran·fenobucarb microemulsion.

[0024] Example 3 30% dinotefuran·fenobucarb microemulsion

[0025] 10% dinotefuran technical, 20% fenobucarb technical, 5% keratinase, 3% fatty alcohol polyoxyethylene ether, 15% N-methylpyrrolidone, 15% solvent oil, 5% propylene glycol, 0.1% fatty acid, and the balance is water, to obtain 30% dinotefuran·fenobucarb microemulsion.

[0026] Example 4 30% dinotefuran·fenobucarb microemulsion

[0027] 10% dinotefuran technical, 20% fenobucarb technical, 6% keratinase, 2% polyoxyethylene polyoxypropylene block copolymer, 20% sec-butyl acetate, 15% methyl oleate, 5% propylene glycol, 0.1% fatty acid, and the balance is water, to obtain 30% dinotefuran·fenobucarb microemulsion.

[0028] Example 5 15% dinotefuran·fenobucarb microemulsion

[0029] Dinotefuran technical 5%, fenobucarb technical 10%, serrapeptase 6%, dodecylphenol polyoxyethylene ether 1.5%, methanol 5%, solvent oil 20%, glycerol 4%, fatty acid 0.05%, balance water, to prepare 15% dinotefuran·fenobucarb microemulsion.

[0030] Example 6 42% dinotefuran·fenobucarb microemulsion

[0031] Dinotefuran technical 14%, fenobucarb technical 28%, glucoamylase 5%, fatty alcohol polyoxyethylene ether 2%, polyoxyethylene-polyoxypropylene block copolymer 1%, N,N-dimethylformamide 8%, cyclohexanone 8%, methyl oleate 15%, ethylene glycol 4%, silicone defoamer 3, balance water, to prepare 42% dinotefuran·fenobucarb microemulsion.

[0032] Example of application two:

[0033] Indoor joint toxicity determination experiment of dinotefuran, fenobucarb and their mixtures against rice planthoppers 1 Test purpose

[0034] To determine the toxicity of dinotefuran, fenobucarb and their different mixed combinations against rice planthoppers indoors, evaluate the synergistic effect, clarify their compatibility, and provide a scientific basis for the research and development of dinotefuran and fenobucarb mixtures.

[0035] 2 Experimental conditions

[0036] 2.1 Test target

[0037] Rice planthoppers (Nilaparvata lugens) are larvae collected from the field and are strains that have been reared on rice indoors for multiple generations. The 3rd instar larvae are used for the bioassay experiment.

[0038] 2. Culture conditions

[0039] The culture conditions for the test target and the target after the test are temperature (25±1)°C, relative humidity 60% - 80%, and light (light-dark ratio 16:8).

[0040] 3 Test design

[0041] 3.1 Test agents

[0042] Dinotefuran 98% technical, fenobucarb 98% technical.

[0043] 3.2 Agent preparation

[0044] Weigh 0.0102 g of dinotefuran 98% technical material. After dissolving it with 0.5 mL of DMF, add 0.4 mL of Tween 80 emulsifier, stir evenly, add clear water to 100 mL to prepare a stock solution of 100 mg / L. Then take 10 mL of the stock solution and add it to 90 mL of water containing 0.1% Tween 80 emulsifier to prepare a test solution of 10 mg / L. Then dilute it with water containing 0.1% Tween 80 emulsifier according to a 2-fold ratio to 5, 2.5, 1.25, 0.625, and 0.3125 mg / L, and conduct tests at a total of 6 concentrations.

[0045] Weigh 0.0204 g of fenobucarb 98% technical material. After dissolving it with 0.5 mL of DMF, add 0.4 mL of Tween 80 emulsifier, stir evenly, add clear water to 200 mL to prepare a stock solution of 100 mg / L. Then take 80 mL of the stock solution and add it to 20 mL of water containing 0.1% Tween 80 emulsifier to prepare a test solution of 80 mg / L. Then dilute it with water containing 0.1% Tween 80 emulsifier according to a 2-fold ratio to 40, 20, 10, 5, and 2.5 mg / L, and conduct tests at a total of 6 concentrations.

[0046] According to the ratios of dinotefuran to fenobucarb of 2:1, 1:1, 1:2, 1:4, and 1:6, respectively measure 13.33, 10, 6.67, 8, and 5.72 mL of the dinotefuran stock solution, and then correspondingly measure 6.67, 10, 13.33, 32, and 34.28 mL of the fenobucarb stock solution. Mix the two, make up the water with emulsifier added to 100 mL, and respectively prepare test solutions of 20, 20, 20, 40, and 40 mg / L. Then dilute them with water containing 0.1% Tween 80 emulsifier according to a 2-fold ratio to 6 concentrations each for testing.

[0047] Add 99.5 mL of water containing 0.1% Tween 80 emulsifier to a beaker containing 0.5 mL of DMF as a blank control.

[0048] 4 Test method

[0049] Refer to the method in the Guidelines for Indoor Bioassay of Pesticides (NY / T 1154.11 - 2008), and adopt the rice stem immersion method.

[0050] 5 Data investigation and statistical analysis

[0051] 5.1 Investigation time and method

[0052] Check the death situation of the test insects after 72 h and record it. The judgment standard for the death of the test insects is that if there is no response when gently touching the test insects with forceps, it is counted as dead.

[0053] 5.2 Evaluation method for synergistic effect

[0054] Referring to the Guidelines for Indoor Bioassay Tests of Pesticides NY / T 1154.7-2006, the co-toxicity coefficient (CTC) of each mixed formulation was calculated using the co-toxicity coefficient method of Sun & Johnson (1960). CTC ≤ 80 indicates antagonistic effect, 80 < CTC < 120 indicates additive effect, and CTC ≥ 120 indicates synergistic effect. The formula for calculating the co-toxicity coefficient is as follows:

[0055]

[0056] The theoretical toxicity index (TTI) of the mixture = toxicity index of pesticide A × percentage of pesticide A in the mixture (%) + toxicity index of pesticide B × percentage of pesticide B in the mixture (%)

[0057]

[0058] 6 Experimental Results

[0059] Table 1 Toxicity determination results of the mixture of dinotefuran and fenobucarb against rice planthoppers

[0060]

[0061] It can be seen from Table 1 of the test results that the test pesticide dinotefuran has good toxicity against rice planthoppers, with an LC 50 value of 2.12 mg / L and an LC 90 value of 9.81 mg / L; the LC 50 value of fenobucarb is 15.66 mg / L, and the LC 90 value is 69.84 mg / L; the toxicities (LC 50 ) of the two pesticides mixed in different ratios against rice planthoppers are 2.40, 3.09, 3.43, 5.32, and 6.08 mg / L respectively, and the co-toxicity coefficients CTC reach 124.10, 120.86, 145.92, 129.26, and 134.68 respectively. It shows that the co-toxicity coefficient (CTC) is the largest and the synergistic effect is the most significant when the mixing ratio of dinotefuran and fenobucarb is 1:2 in the test design. Application Example 3: Field efficacy test of Examples 1 - 3 for controlling rice planthoppers 1 Test objective

[0062] To verify the control effect of the 30% dinotefuran·fenobucarb microemulsion developed by the applicant against rice planthoppers and its safety to rice, clarify the field application dosage and application technology, and provide a scientific basis for pesticide registration.

[0063] 2 Test basis

[0064] "Good Laboratory Practice for Pesticide Registration Tests", "GB / T 17980.4 - 2000 Guidelines for Field Efficacy Tests of Pesticides (Part 1) Insecticides for Controlling Rice Planthoppers".

[0065] 3 Experimental site

[0066] This experiment was arranged in Anhui Province and Shaanxi Province.

[0067] 4 Selection of Test Objects, Crops and Varieties

[0068] Test object: Rice planthoppers.

[0069] 5 Experimental Design and Arrangement

[0070] 5.1 Dosage and Numbering of Medicaments

[0071] Table 2 Experimental Design of Tested Medicaments

[0072]

[0073] 5.2 Application Time and Frequency

[0074] When the rice was in the jointing stage, plant spraying was used, and the medicament was applied 1 time in total.

[0075] 5.3 Application Volume

[0076] The dosage of the liquid medicine was 750 liters per hectare.

[0077] 5.4 Investigation Time and Frequency

[0078] 5.4.1 Investigation frequency for Field Tests 1 and 2: The population base was investigated before medicament application, and the population quantity was investigated 3, 7, and 14 days after medicament application, for a total of 4 times.

[0079] 5.4.2 Investigation frequency for Field Tests 3 and 4: The population base was investigated before medicament application, and the population quantity was investigated 3 and 7 days after medicament application, for a total of 3 times.

[0080] 5.5 Calculation Method of Medicament Efficacy The control effect was calculated according to the formula in the "Guidelines for Field Efficacy Trials of Pesticides" for field medicament efficacy.

[0081]

[0082] PT: Control effect (%)

[0083] PT1: Insect quantity after medicament application in the medicament treatment area

[0084] PT0: Insect quantity before medicament application in the medicament treatment area

[0085] CK1: Insect quantity after medicament application in the blank control area

[0086] CK0: Insect quantity before medicament application in the blank control area

[0087] 6 Experimental Results

[0088] Table 3 Results of Field Efficacy Trials for Controlling Rice Planthoppers in Examples 1 - 2

[0089]

[0090] The experimental results show that: for the test substance of 30% dinotefuran·fenobucarb microemulsion in Example 1 or 2 against rice planthoppers, when applying the medicine at the early peak stage of the low-instar nymphs of rice planthoppers and spraying at an active ingredient dosage of 225 g / ha, it can be seen from Table 3 that after 3 days, 7 days, and 14 days, the control effects are significantly better than those of the control agents. The insecticidal effect reaches over 86% after 3 days, over 91% after 7 days, and the control effect reaches over 90% after 14 days. Compared with the control single agents, there are significant differences at the 0.01 and 0.05 levels. Therefore, it shows that the test substance of 30% dinotefuran·fenobucarb microemulsion has quick-acting and long-lasting effects, and is safe for crops and applicators throughout the experimental period.

[0091] Table 4 Field efficacy test results of Examples 3 - 4 for controlling rice planthoppers

[0092]

[0093]

[0094] The experimental results show that: for the test substance of 30% dinotefuran·fenobucarb microemulsion in Example 3 or 4 against rice planthoppers, when applying the medicine at the early peak stage of the low-instar nymphs of rice planthoppers and spraying at an active ingredient dosage of 225 g / ha, it can be seen from Table 4 that after 3 days and 7 days, the control effects are significantly better than those of the control agents. The insecticidal effect reaches over 86% after 3 days and over 90% after 7 days. Compared with the control single agents, there are significant differences at the 0.01 and 0.05 levels. Therefore, it shows that the test substance of 30% dinotefuran·fenobucarb microemulsion has quick-acting effects, and is safe for crops and applicators throughout the experimental period.

[0095] Application Example Four: (Examples 1 - 4) Experiment on environmental impact determination

[0096] Our company commissioned a third-party company to conduct 10 tests on Examples 1 - 4, including acute oral toxicity test for bees, acute contact toxicity test for bees, acute oral toxicity test for birds, acute toxicity test for fish, acute toxicity test for silkworms, acute activity inhibition test for daphnia, algal growth inhibition test, acute toxicity test for earthworms, acute toxicity test for natural enemy trichogramma, and acute contact toxicity test for natural enemy ladybugs. The test results are as follows:

[0097] 1) Acute oral toxicity test for bees, the toxicity level is low toxicity.

[0098] 2) Acute contact toxicity test for bees, the toxicity level is low toxicity.

[0099] 3) Acute oral toxicity test for birds, the toxicity level is slightly toxic.

[0100] 4) Acute toxicity test on fish, with the toxicity level being low toxicity.

[0101] 5) Acute toxicity test on silkworms, with the toxicity level being low toxicity.

[0102] 6) Acute activity inhibition test on Daphnia, with the toxicity level being low toxicity.

[0103] 7) Algae growth inhibition test, with the toxicity level being low toxicity.

[0104] 8) Acute toxicity test on earthworms, with the toxicity level being slightly toxic.

[0105] 9) Acute toxicity test on the natural enemy Trichogramma, with low risk.

[0106] 10) Acute contact toxicity test on the natural enemy ladybug, with low risk.

[0107] Application Example Five: Acute toxicology determination experiments of Examples 1 to 4

[0108] Our company commissioned a third-party testing agency to conduct acute toxicity determination tests on Examples 1 to 2, and the test results are as follows:

[0109] 1) Acute oral toxicity test on rats, with the toxicity levels all being slightly toxic.

[0110] 2) Acute percutaneous toxicity test on rats, with the toxicity level being slightly toxic.

[0111] 3) Rabbit eye irritation test, without irritation.

[0112] 4) Skin irritation test, without irritation.

[0113] 5) Skin sensitization test. Experimental animal: guinea pig; Sensitization intensity: none.

[0114] The above content is a further detailed description of the present invention in combination with specific preferred implementation manners. It cannot be determined that the specific implementation of the present invention is only limited to these descriptions. For those of ordinary skill in the technical field to which the present invention belongs, without departing from the concept of the present invention, several simple deductions or substitutions can still be made, and all should be regarded as belonging to the protection scope of the present invention.

Claims

1. An insecticidal composition containing dinotefuran, characterized in that: The described insecticidal composition contains 9 - 51% of active ingredient, 1.5 - 15% of synergist, 5 - 70% of adjuvant, and the balance of water to make 100%. The active ingredient is dinotefuran and fenobucarb, and the weight ratio of dinotefuran to fenobucarb is 1:

2. The synergist is a mixture of bio - enzyme and high - molecular emulsifier, and the weight ratio of bio - enzyme to high - molecular emulsifier is 7:1 - 1:

1.

2. The pesticidal composition according to claim 1, characterized in that: The synergist is a mixture of bio - enzyme and high - molecular emulsifier, and the weight ratio of bio - enzyme to high - molecular emulsifier is 5:1 - 1:1, and the optional weight ratio is 3:1 - 1:

1.

3. The pesticidal composition according to claim 1, wherein: The adjuvant contains one or more of solvent, antifreeze, and defoamer.

4. The pesticidal composition according to any one of claims 1 to 3, characterized in that: The insecticidal composition contains: 3 - 17% of dinotefuran, 6 - 34% of fenobucarb, 2 - 10% of synergist, 6 - 50% of adjuvant, and the balance of water.

5. The pesticidal composition according to claim 4, wherein: The insecticidal composition contains: 5 - 12% of dinotefuran, 10 - 24% of fenobucarb, 2 - 7% of synergist, 5 - 40% of solvent, 1 - 7% of antifreeze, 0.05 - 1% of defoamer, and the balance of water.

6. The pesticidal composition according to claim 5, characterized in that: The insecticidal composition contains: 10% of dinotefuran, 20% of fenobucarb, 5% of synergist, 10 - 30% of solvent, 2 - 6% of antifreeze, 0.05 - 0.5% of defoamer, and the balance of water.

7. The pesticidal composition according to any one of claims 1 to 6, characterized in that: Application of the insecticidal composition in controlling rice planthoppers.

8. The pesticidal composition according to claim 7, wherein: When the insecticidal composition is used to control rice planthoppers, it is applied at the early peak stage of the low - instar nymphs of rice planthoppers, and sprayed once according to the dosage of active ingredient of 100 - 300 g / ha.

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