Preparation and biological evaluation method of porous titanium alloy medical implant

Abstract

The invention belongs to the technical field of medical implant preparation, and particularly relates to a preparation and biological evaluation method of a porous titanium alloy medical implant. The preparation method of the porous titanium alloy medical implant comprises the following steps: S1, preparing a porous titanium artificial tooth root implant; s2, preparing a hydroxyapatite coating of the porous titanium artificial tooth root implant by adopting an electrochemical method; s3, carrying out fatigue test on the porous titanium artificial tooth root implant with the hydroxyapatite coating; s4, preparing a composite nano coating of the porous titanium artificial tooth root implant by adopting an electrochemical method; and S5, carrying out physicochemical and biological analysis on the obtained porous titanium artificial tooth root implant with the composite nano coating. The composite nano coating is applied to surface modification of the porous titanium alloy, the advantages of respective technologies are exerted, osteoblast proliferation and differentiated protein molecules can be remarkably induced, bone induction and bone conduction are enhanced, and bone defect repair and angiogenesis are promoted.

Description

Technical Field

[0001] The present invention belongs to the technical field of medical implant preparation, and particularly relates to a preparation and biological evaluation method for a porous titanium alloy medical implant. Background Art

[0002] As a part of a medical implant implanted into bone tissue to replace the natural tooth root, porous titanium alloy has the functions of retention, support and force conduction. Electron beam melting technology is a newly emerging advanced metal rapid prototyping manufacturing technology in recent years, which can achieve controllable manufacturing from microstructures to macrostructures and realize effective transfer of the load at the material-bone interface.

[0003] In vivo, the biological activity of the titanium surface is relatively low, and osteoblast adhesion and proliferation cannot be induced to form osseointegration in the short term. Poor osseointegration may even lead to loosening and falling off of the implant, affecting the long-term repair effect. Therefore, surface bioactivation modification treatment is required.

[0004] Typical nano-coatings for surface bioactivation modification treatment of porous titanium alloy implants include hydroxyapatite [HA, Ca10(PO4)6(OH)2, HA], graphene oxide (GO), recombinant human bone morphogenetic protein 2 (rh-BMP2), and chitosan and other nanomaterials. Hydroxyapatite has an inorganic mineral composition similar to that of human bone tissue and has been widely used in the field of medical biomaterials; however, the disadvantages of hydroxyapatite coatings such as high brittleness, low strength, and poor adhesion limit their application in implant coating materials. Graphene oxide has excellent properties in terms of electrical, mechanical, and thermal properties, and its oxygen-containing groups have characteristics such as dispersibility and hydrophilicity. However, the binding force between graphene oxide and the carrier is weak. Based on the problems of weak binding force between the existing porous titanium alloy surface modification coatings and the carrier, poor coating corrosion resistance, and poor biological activity, there is an urgent need for a preparation and biological evaluation method for porous titanium alloy medical implants, and then to study the mechanism of the modified nano-composite coating of porous titanium-zirconium alloy implants on bone immune regulation and osteogenesis, and to explore the application from physical and chemical properties to biological research to the final implant surface modification, so as to provide a theoretical basis and technical support for the construction of implant surface modification materials and their applications. Summary of the Invention

[0005] Aiming at the above problems, the purpose of the present invention is to provide a preparation and biological evaluation method for a porous titanium alloy medical implant to study the mechanism of the modified nano-composite coating of porous titanium-zirconium alloy implants on bone immune regulation and osteogenesis.

[0006] To achieve the above purpose, the present invention adopts the following technical solutions:

[0007] On the one hand, the present invention provides a method for preparing a porous titanium alloy medical implant, comprising the following steps:

[0008] Step S1: Prepare a porous titanium artificial tooth root implant;

[0009] Step S2: Prepare a hydroxyapatite coating on the porous titanium artificial tooth root implant by an electrochemical method;

[0010] Step S3: Conduct a fatigue test on the porous titanium artificial tooth root implant with a hydroxyapatite coating;

[0011] Step S4: Prepare a composite nano - coating on the porous titanium artificial tooth root implant by an electrochemical method;

[0012] Step S5: Conduct physical, chemical and biological analyses on the obtained porous titanium artificial tooth root implant with a composite nano - coating.

[0013] In a possible implementation manner, in step S1, the preparation process of the porous titanium artificial tooth root implant is as follows:

[0014] Establish a three - dimensional model of the artificial tooth root implant;

[0015] Obtain titanium alloy powder with a diameter of 40 - 70 μm by ultrasonic gas atomization powder making method;

[0016] Use an electron beam melting forming device to layer - by - layer print and stack multiple porous titanium artificial tooth root implants along the forming path.

[0017] In a possible implementation manner, the printing trajectory of the electron beam melting forming device is non - linear, the directions of the printing trajectories of each layer are different, and the horizontal projections of the printing connection nodes of each layer do not coincide, so that the printing microstructure of the porous titanium artificial tooth root implant is a spatial network structure with non - linear through - pores.

[0018] In a possible implementation manner, the porous titanium artificial tooth root implant includes a body, the body is a hollow cone, an external thread is provided on the outer surface of the body, and an internal thread is provided on the inner surface of the body.

[0019] In a possible implementation manner, in step S2, the process of preparing the hydroxyapatite coating on the porous titanium artificial tooth root implant by an electrochemical method is as follows:

[0020] The electrolytic cell contains an electrolyte solution, with a graphite electrode as the anode and the porous titanium artificial tooth root implant as the cathode. The anode and the cathode are connected to a DC power supply, and the process parameters are adjusted to prepare the hydroxyapatite coating on the porous titanium artificial tooth root implant.

[0021] In a possible implementation manner, in step S3, the fatigue test of the porous titanium dental root implant is completed by using an accelerated life fatigue test device. In the regions with high strain and large changes in strain gradient, partial hydroxyapatite coating exfoliates. Different times of the accelerated life fatigue test result in different exfoliation situations of the hydroxyapatite coating.

[0022] In a possible implementation manner, in step S4, with a graphite electrode as the anode and porous titanium dental root implants with different remaining percentages of hydroxyapatite coating as the cathode, the process parameters are adjusted to prepare a composite nano - coating on the surface of the porous titanium dental root implant.

[0023] In a possible implementation manner, the composite nano - coating is a hydroxyapatite - graphene oxide composite coating, a hydroxyapatite - silver coating, or a hydroxyapatite - magnesium oxide coating.

[0024] On the other hand, the present invention provides a biological evaluation method for a porous titanium alloy medical implant obtained by the method described above, including the following steps:

[0025] Step N1: Prepare a bone defect model using New Zealand rabbits for in - vivo research;

[0026] Step N2: After the New Zealand rabbits are successfully anesthetized, avoid subcutaneous blood vessels, expose the lateral epicondyle of the femur, strip the attached tendon, and expose the metaphyseal line of the femoral shaft and femoral condyle;

[0027] Step N3: Drill holes in the exposed femoral shaft using a dental drill;

[0028] Step N4: Screw the porous titanium dental root implant into the drilled hole;

[0029] Step N5: Observe the bone defect and bone repair conditions of the New Zealand rabbits.

[0030] In a possible implementation manner, in step N5, micro - CT is used to observe the situation of new bone starting to grow into the porous material and the percentage of bone combined with the porous material over time.

[0031] The advantages and positive effects of the present invention are as follows: A preparation method of a porous titanium alloy medical implant provided by the present invention prepares a porous titanium alloy medical implant through an electron beam melting metal rapid prototyping technology, achieving precise control of the pore size and pore structure, and the pores are interconnected and penetrated, greatly improving the biocompatibility of the material; hydroxyapatite and graphene oxide are compounded and applied to the surface modification of the porous titanium alloy, and bone morphogenetic protein is attached. Graphene oxide has excellent properties in terms of electrical, mechanical and thermal properties, and its oxygen-containing groups have characteristics such as dispersibility and hydrophilicity, which can serve as activation sites and provide binding sites for preparing composite materials in combination with other materials. Hydroxyapatite and graphene oxide play the advantages of their respective technologies, can significantly induce protein molecules for the proliferation and differentiation of osteoblasts, enhance bone induction and bone conduction, promote the repair of bone defects and angiogenesis, and solve the problems of weak binding force between the coating and the carrier of the existing surface modification of porous titanium alloy, poor corrosion resistance and biological activity of the coating.

[0032] The present invention provides a preparation and biological evaluation method of a porous titanium alloy medical implant, and further studies the preparation of a porous titanium-zirconium alloy implant and a surface modification composite coating, and the bone immune regulation process and its mechanism in osteogenesis after the implant is implanted into the organism, and explores the application from physical and chemical properties to biological research to the final surface modification of the implant, providing a theoretical basis and technical support for constructing surface modification materials of the implant and their applications.

[0033] Other features and advantages of the present invention will be described in the following specification, and in part will be obvious from the specification, or will be understood by implementing the present invention. The objectives and other advantages of the present invention can be achieved and obtained by the structures specifically pointed out in the written specification and the drawings.

[0034] The technical solutions of the present invention will be further described in detail below through the drawings and embodiments. Description of the Drawings

[0035] The drawings are used to provide a further understanding of the present invention, and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation to the present invention. In the drawings:

[0036] Figure 1 It is a schematic diagram of the electrochemical principle of a porous titanium artificial tooth root implant in an embodiment of the present invention;

[0037] Figure 2 It is an axonometric view of multiple porous titanium artificial tooth root implants in an embodiment of the present invention;

[0038] Figure 3 It is a schematic sectional view of a porous titanium artificial tooth root implant in an embodiment of the present invention;

[0039] Figure 4 Schematic diagram of the microscopic single-layer printing of the porous titanium artificial tooth root implant in the embodiment of the present invention;

[0040] Figure 5 Side view of the microscopic three-layer printing of the porous titanium artificial tooth root implant in the embodiment of the present invention;

[0041] Figure 6 Axonometric view of the microscopic three-layer printing of the porous titanium artificial tooth root implant in the embodiment of the present invention;

[0042] Figure 7 Top view of the microscopic three-layer printing of the porous titanium artificial tooth root implant in the embodiment of the present invention;

[0043] Figure 8 Schematic diagram of the structure of the accelerated life fatigue test device in the embodiment of the present invention;

[0044] Figure 9 Schematic diagram of the strain analysis of the porous titanium artificial tooth root implant under the working load in the embodiment of the present invention.

[0045] In the figure: 1 - electrolytic cell, 2 - electrolyte, 3 - graphite electrode, 4 - DC power supply, 5 - base, 6 - axial vibration generator, 7 - cross beam, 8 - column, 20 - porous titanium artificial tooth root implant, 22 - bottom plate, 23 - body, 24 - external thread, 25 - internal thread, 26 - printing track, 27 - first-layer printing track, 28 - second-layer printing track, 29 - third-layer printing track, 30 - three-dimensional boundary, 31 - non-linear pine needles. Detailed implementation manners

[0046] In the present invention, unless otherwise clearly defined and limited, the terms "installation", "connection", "connection", "fixation" and other terms should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, and it can be the communication inside two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0047] The following is a description of the preferred embodiments of the present invention with reference to the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0048] See Figures 1 to 9 As shown, an embodiment of the present invention provides a preparation method for a porous titanium alloy medical implant, including the following steps:

[0049] Step S1: Prepare the porous titanium artificial tooth root implant 20;

[0050] Step S2: Prepare a hydroxyapatite coating on the porous titanium artificial tooth root implant 20 by an electrochemical method;

[0051] Step S3: Conduct a fatigue test on the porous titanium artificial tooth root implant 20 with a hydroxyapatite coating;

[0052] Step S4: Continue to prepare a composite nano - coating on the porous titanium artificial tooth root implant 20 with different remaining percentages of the hydroxyapatite coating by an electrochemical method;

[0053] Step S5: Conduct physical - chemical and biological analyses on the obtained porous titanium artificial tooth root implant 20 with a composite nano - coating.

[0054] In the embodiment of the present invention, in Step S1, the preparation process of the porous titanium artificial tooth root implant 20 is as follows:

[0055] Use CAD software to establish a three - dimensional model of the artificial tooth root implant;

[0056] Obtain titanium alloy powder with a diameter of 40 - 70 μm by an ultrasonic gas atomization powder - making method;

[0057] Use an Arcam A1 type electron beam melting and forming device to layer - by - layer print and stack a plurality of porous titanium artificial tooth root implants 20 on the bottom plate 22 along the forming path, achieving the purpose of high - throughput printing of the porous titanium artificial tooth root implant 20. When in use, each porous titanium artificial tooth root implant 20 is cut by a slow - wire cutting machine. In this embodiment, the porosity of the porous titanium artificial tooth root implant 20 is 30%, the diameter is 5 mm, and the height is 7 mm.

[0058] See Figure 3 and Figure 6 As shown, in the embodiment of the present invention, the printing track 26 of the electron beam melting and forming device is non - linear. The multiple printing tracks 26 in the same layer are parallel to each other and have non - linear gaps; the directions of the printing tracks 26 in each layer are different, and the horizontal projections of the printing connection nodes in each layer do not overlap, so that the printed microstructure of the porous titanium artificial tooth root implant 20 has non - linearly penetrating pores, forming a spatial network structure.

[0059] Specifically, as shown in Figures 5 to 7 Taking three - layer printing tracks as an example, the directions of the first - layer printing track 27, the second - layer printing track 28, and the third - layer printing track 29 are different, and the horizontal projections of the printing connection nodes between each layer do not overlap. Therefore, the printed microstructure of the porous titanium artificial tooth root implant 20 has non - linearly penetrating pores, that is, all the pores in each layer are spatially connected up and down, and the printing nodes of the printing tracks 26 in the upper and lower layers do not overlap. This layout can achieve the anisotropic effect in the direction of tooth - generated pressure and other directions.

[0060] See Figure 7 As shown in Figure 7 , in the embodiment of the present invention, outside the three-dimensional boundary 30 of the porous titanium artificial tooth root implant 20, there are non-linear pine needles 31, and the non-linear pine needles 31 can effectively penetrate into the mandible, having an enhanced fixation effect.

[0061] See Figure 2 and Figure 3 As shown in Figure 2 and Figure 3 , in the embodiment of the present invention, the porous titanium artificial tooth root implant 20 includes a body 23. The body 23 is a hollow cone, and the outer surface of the body 23 is provided with an external thread 24, and the inner surface of the body 23 is provided with an internal thread 25. The external thread 24 is used for fixing with the mandible, and the internal thread 25 is used for locking with the abutment and the dental crown. Since each person's mandible is different, the multiple porous titanium artificial tooth root implants 20 on the printed substrate 22 are different, and the specific dimensions and lengths are customized according to the three-dimensional CT modeling of the patient's mandible.

[0062] See Figure 1 As shown in Figure 1 , in step S2, the process of preparing the hydroxyapatite (HA) coating of the porous titanium artificial tooth root implant 20 by the electrochemical method is as follows:

[0063] The electrolytic cell 1 contains an electrolyte 2. Using the graphite electrode 3 as the anode and the porous titanium artificial tooth root implant 20 as the cathode, the anode and the cathode are connected to a DC power supply 4, and the process parameters are adjusted to prepare the hydroxyapatite coating of the porous titanium artificial tooth root implant 20.

[0064] In step S3, the fatigue test of the porous titanium artificial tooth root implant 20 is completed by an accelerated life fatigue test device. Utilizing the characteristics of the HA coating being more brittle, having relatively low strength, and poor adhesion, part of the HA coating falls off. The characteristics of the falling-off are the falling-off in the high-strain region and the region with large strain gradient changes. Different times of the accelerated life fatigue test result in different remainders of the HA coating of the porous titanium artificial tooth root implant 20 with the HA coating;

[0065] See Figure 8 As shown in Figure 8 , in the embodiment of the present invention, the accelerated life fatigue test device includes a base 5, an axial vibrator 6, a cross beam 7, and columns 8. Among them, the cross beam 7 is arranged parallel to the upper part of the base 5, and both sides are connected to the base 5 through the columns 8. The axial vibrator 6 is arranged below the cross beam 7. The porous titanium artificial tooth root implant 20 is placed on the base 5, and a vibration load is applied to the top of the porous titanium artificial tooth root implant 20 through the axial vibrator 6 to conduct the accelerated life fatigue test.

[0066] In step S4, using the graphite electrode 3 as the anode and the porous titanium artificial tooth root implants 20 with different remaining percentages of the HA coating as the cathode, the process parameters are adjusted to prepare a composite nano-coating on the surface of the porous titanium artificial tooth root implant 20.

[0067] Specifically, the composite nano-coating is a hydroxyapatite-graphene oxide composite coating (HA-GO), a hydroxyapatite-silver (HA-Ag) coating, or a hydroxyapatite-magnesium oxide (HA-MgO) coating.

[0068] The electrolyte 2 can be replaced with different solutions according to process requirements, such as electrolytes like HA, HA-GO, HA-Ag, and HA-MgO.

[0069] In step S5, the physical, chemical, and biological analyses include, but are not limited to, the microstructure, surface morphology, and surface roughness of the alloy and the coating, and analyze the influence of process parameters on the coating morphology. X-ray photoelectron spectroscopy is used to qualitatively analyze the elemental composition of the material surface. An electrochemical workstation and a multi-functional material surface performance tester are used to analyze the corrosion resistance, bonding strength, and mechanical strength of the coating; the solid-liquid contact angle method is used to analyze the hydrophilic and hydrophobic properties of the coating; Escherichia coli and Staphylococcus aureus are used to test the antibacterial properties of the coating; and an enzyme-labeled instrument is used to detect the absorbance values of various ions in the soaking solution to investigate the physiological stability of the coating.

[0070] See Figure 9 As shown, strain analysis of the porous titanium artificial tooth root implant 20 under working load:

[0071] When the porous titanium artificial tooth root implant 20 is under working load, such as during chewing, due to its internal space reticular structure, the strains at different surface parts of the porous titanium artificial tooth root implant 20 are different. Due to the relatively large brittleness, low strength, and poor bonding of the HA layer, part of the HA coating will fall off, especially in the high-strain area and the variable-strain area, while it is not easy to fall off in the low-strain area. Therefore, for the nano-composite coating, first apply the HA coating, and then conduct accelerated life fatigue tests to different degrees, and then implants with different percentages of the remaining HA coating can be obtained. Then apply the G0 (graphene oxide) coating to form a composite coating. In this way, the distribution of the HA coating and the GO coating is controlled at the micro-morphology level. Different proportions of the HA-GO composite nano-coating result in different biological effects.

[0072] The present invention provides a method for preparing a porous titanium alloy medical implant. The porous titanium alloy medical implant is prepared by an electron beam melting metal rapid prototyping technology, which enables precise control of the pore size and pore structure, and the pores are interconnected and continuous, greatly improving the biocompatibility of the material. Hydroxyapatite and graphene oxide are compounded and applied to the surface modification of the porous titanium alloy, and bone morphogenetic protein is attached. Graphene oxide has excellent properties in terms of electrical, mechanical and thermal properties, and its oxygen-containing groups have characteristics such as dispersibility and hydrophilicity, which can serve as activation sites and provide binding sites for preparing composite materials in combination with other materials. Hydroxyapatite and graphene oxide play the advantages of their respective technologies, and are expected to significantly induce protein molecules for the proliferation and differentiation of osteoblasts, enhance bone induction and bone conduction, promote the repair of bone defects and angiogenesis, and solve the problems of weak binding force between the coating and the carrier of the existing porous titanium alloy surface modification, poor corrosion resistance and biological activity of the coating.

[0073] Another embodiment of the present invention provides a biological evaluation method for a porous titanium alloy medical implant, comprising the following steps:

[0074] Step N1: Using New Zealand rabbits to prepare a bone defect model for in vivo research;

[0075] Step N2: After the New Zealand rabbits are successfully anesthetized, avoid the subcutaneous blood vessels, expose the lateral epicondyle of the femur, strip the attached tendon, and expose the femoral shaft and the metaphyseal line of the femoral condyle;

[0076] Step N3: Drill holes in the exposed femoral shaft using a dental drill;

[0077] Specifically, a handheld dental drill with a diameter of 6 mm is used, and the drilling depth is 1 cm to remove the bone mass and the periosteum at the defect site together;

[0078] Step N4: Screw a porous titanium artificial tooth root implant 20 into the drilled hole;

[0079] Step N5: Observe the bone defect and bone repair conditions of the New Zealand rabbits through technical means such as imaging and histology; specifically, use micro-computed tomography (Micro-CT) to observe the situation of new bone growing into the porous material and the percentage of bone combined with the porous material over time.

[0080] The present invention establishes a method for preparing and biologically evaluating a porous titanium alloy medical implant, and further studies the porous titanium-zirconium alloy implanted in the body, the mechanism of the modified nano-composite coating of the porous titanium-zirconium alloy implant on bone immune regulation and its osteogenic aspect, and explores the application from physical and chemical properties to biological research to the final surface modification of the implant, providing a theoretical basis and technical support for constructing implant surface modification materials and their applications.

[0081] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalent technologies, the present invention is also intended to include these modifications and variations.

Claims

1. A method for preparing a porous titanium alloy medical implant, characterized in that: The following steps are involved: Step S1: preparing a porous titanium artificial tooth root implant (20); Step S2: preparing a hydroxyapatite coating of a porous titanium artificial tooth root implant (20) by an electrochemical method; Step S3: performing fatigue testing on the porous titanium artificial tooth root implant (20) having a hydroxyapatite coating; Step S4: preparing a composite nano-coating of the porous titanium artificial tooth root implant (20) by an electrochemical method; Step S5: Performing physical, chemical and biological analyses on the obtained porous titanium artificial tooth root implant (20) with the composite nano-coating.

2. The method for preparing a porous titanium alloy medical implant according to claim 1, characterized in that: In step S1, the preparation process of the porous titanium artificial tooth root implant (20) is as follows: Create a three-dimensional model of an artificial tooth root implant; Titanium alloy powder with a diameter of 40 to 70 μm is obtained by ultrasonic gas atomization powder making method; Electron beam melting molding equipment is used to print and stack a plurality of porous titanium artificial tooth root implants (20) layer by layer along a molding path.

3. The method for preparing a porous titanium alloy medical implant according to claim 2, characterized in that: The printing track (26) of the electron beam melting molding device is non-linear, the directions of the printing tracks (26) of each layer are different, and the horizontal projections of the printed connection nodes of each layer do not overlap, so that the printed microstructure of the porous titanium artificial tooth root implant (20) is a spatial mesh structure with non-linear through-holes.

4. The method for preparing a porous titanium alloy medical implant according to claim 2, characterized in that: The porous titanium artificial tooth root implant (20) comprises a body (23), which is a hollow conical body. The outer surface of the body (23) is provided with an external thread (24), and the inner surface of the body (23) is provided with an internal thread (25).

5. The method for preparing a porous titanium alloy medical implant according to claim 1, characterized in that: In step S2, the process of preparing the hydroxyapatite coating of the porous titanium artificial tooth root implant (20) by electrochemical method is as follows: The electrolytic cell (1) contains an electrolyte (2), a graphite electrode (3) is used as an anode, a porous titanium artificial tooth root implant (20) is used as a cathode, the anode and the cathode are connected to a direct current power supply (4), and the process parameters are adjusted to prepare a hydroxyapatite coating of the porous titanium artificial tooth root implant (20).

6. The method for preparing a porous titanium alloy medical implant according to claim 1, characterized in that: In step S3, fatigue testing of the porous titanium artificial tooth root implant (20) is performed using an accelerated life fatigue test device, and the hydroxyapatite coating partially falls off in areas with high strain and large strain gradient changes. The hydroxyapatite coating falls off in different conditions at different times of the accelerated life fatigue test.

7. The method for preparing a porous titanium alloy medical implant according to claim 1, characterized in that: In step S4, the graphite electrode (3) is used as the anode and the porous titanium artificial tooth root implant (20) with different remaining percentages of hydroxyapatite coating is used as the cathode, and the process parameters are adjusted to prepare a composite nano coating on the surface of the porous titanium artificial tooth root implant (20).

8. The method for preparing a porous titanium alloy medical implant according to claim 7, characterized in that: The composite nano coating is a hydroxyapatite-graphene oxide composite coating, a hydroxyapatite-silver coating or a hydroxyapatite-magnesium oxide coating.

9. A biological evaluation method for a porous titanium alloy medical implant obtained by the method according to any one of claims 1 to 8, characterized in that: The following steps are involved: Step N1: Use New Zealand rabbits to prepare a bone defect model for in vivo research; Step N2: After the New Zealand rabbit is anesthetized successfully, avoid the subcutaneous blood vessels, expose the lateral epicondyle of the femur, peel off the attached tendon, and expose the femoral shaft and the femoral condyle epiphyseal line; Step N3: drilling a hole in the exposed femoral shaft using a dental drill; Step N4: screwing the porous titanium artificial tooth root implant (20) into the drilled hole; Step N5: Observe the bone defect and bone repair of New Zealand rabbits.

10. The biological evaluation method of porous titanium alloy medical implant according to claim 9, characterized in that: In step N5, micro CT is used to observe the growth of new bone into the porous material and the percentage of bone integration with the porous material over time.

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