Preparation method and application of semen cuscutae skin care cream

Abstract

The invention belongs to the technical field of skin care cosmetics, and particularly relates to a preparation method and application of semen cuscutae skin care cream, and the preparation method comprises the following steps: (1) raw material treatment: mixing semen cuscutae, raspberry and lotus seedpod according to the weight part ratio of (1-10): (1-5): (1-5), cleaning, selecting and weighing; (2) extracting: soaking the raw materials in a 30% ethanol solution in proportion, extracting for 1-2 times at normal temperature under a high-pressure condition, and collecting an extracting solution; (3) purification: performing high-speed centrifugation on the extracting solution, filtering for three times, and concentrating to obtain a traditional Chinese medicine extract; (4) emulsifying: mixing the traditional Chinese medicine extract with 0.1-0.5% of azone and orange peel essential oil, adding purified water to form a water phase, stirring or homogenizing and emulsifying the water phase and an oil phase component, cooling and discharging; and (5) inspecting and packaging: inspecting the semi-finished product, and packaging the qualified semi-finished product into a finished product. The traditional Chinese medicine skin care cream has enhanced effects of preventing ultraviolet damage and resisting light aging, does not have toxic or side effects and adverse reactions such as liver, kidney and heart damage after long-term application, and has remarkable effects.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care cosmetics, and specifically relates to a preparation method and application of dodder skin care cream. Background Art

[0002] Skin sunburn is an acute traumatic skin reaction caused by the skin receiving strong light irradiation. The affected skin shows symptoms such as redness, swelling, burning, pain, and even blisters, burning pain, skin peeling, etc. Some patients may also have systemic symptoms such as headache, fever, nausea, and vomiting.

[0003] Skin dryness refers to the phenomenon that the skin lacks moisture and makes people feel uncomfortable. Its symptoms are mainly skin tightness, dry peeling in individual parts, and itching all over the body after taking a bath. Aging, climate change, lack of sleep, excessive fatigue, too hot bath water, and strong alkaline detergents are all important reasons for skin dryness.

[0004] In the hot summer, people often need skin care products that can provide sun protection to prevent skin sunburn. At the same time, after mild sunburn, these products can not only provide subsequent sun protection but also play a certain role in repair. However, at present, various skin care products on the market have single functions, and the comprehensive effects of common ordinary moisturizing creams are not ideal. There are few skin care products with comprehensive functions. Some contain ingredients such as hormones or synthetic antimicrobial drugs, and some contain mercury, which cause great harm to the human body if used for a long time.

[0005] Since Chinese herbal medicines have unique advantages in skin care and beauty, especially now people have begun to abandon chemical compounds and advocate natural substances. Chinese herbal medicine skin care and beauty have been welcomed by people. However, at present, the production price of Chinese medicine skin care products on the market is high, the content of active ingredients is low, and the product quality is unstable. Therefore, we propose a preparation method and application of dodder skin care cream. Summary of the Invention

[0006] The present invention aims to solve one of the technical problems existing in the prior art or related technologies.

[0007] For this reason, the technical solution adopted by the present invention is as follows:

[0008] A preparation method of dodder skin care cream, comprising the following steps:

[0009] (1) Raw material treatment: Mix dodder, raspberry, and lotus seeds in a weight ratio of 1-10:1-5:1-5, then perform cleaning and selection, and weighing;

[0010] (2) Extraction: Soak the raw materials in a 30% ethanol solution according to a certain ratio, and then perform extraction at normal temperature under high pressure for 1-2 times, and collect the extraction solution;

[0011] (3) Purification: Centrifuge the extraction solution at high speed, filter it three times, and then concentrate it to obtain a Chinese medicine extract;

[0012] (4) Emulsification: Mix the Chinese medicine extract with 0.1 - 0.5% azone and orange peel essential oil, add purified water to form an aqueous phase, and emulsify it with the oil phase components by stirring or homogenization, then discharge after cooling;

[0013] (5) Inspection and packaging: Inspect the semi-finished product, and package it as a finished product after passing the inspection.

[0014] In a preferred example of the present invention, it can be further configured that: in step (2), the pressure range of the high-pressure condition is 0.2 - 0.5 MPa, and the extraction time is 30 - 60 minutes per time.

[0015] In a preferred example of the present invention, it can be further configured that: in step (3), the rotation speed of the high-speed centrifugation is 8000 - 12000 rpm, and the centrifugation time is 10 - 15 minutes.

[0016] In a preferred example of the present invention, it can be further configured that: in step (4), the oil phase components include vegetable oils and emulsifiers, and the vegetable oils are selected from at least one of jojoba oil, olive oil or shea butter.

[0017] In a preferred example of the present invention, it can be further configured that: the addition amount of the orange peel essential oil is 0.1 - 0.3%, and the orange peel essential oil is extracted from citrus peels by steam distillation.

[0018] In a preferred example of the present invention, it can be further configured that: for the Cuscutae semen skincare cream prepared by the above method, its active ingredient includes chlorogenic acid, the chlorogenic acid content ≥ 5 mg / g, and the ultraviolet absorption rate of the skincare cream ≥ 85%.

[0019] In a preferred example of the present invention, it can be further configured that: the Cuscutae semen skincare cream prepared by the above method is used for preventing or treating skin photoaging, and the skincare cream has been verified by toxicology experiments to have no toxicity to the liver, kidney and heart and no skin irritation.

[0020] In a preferred example of the present invention, it can be further configured that: the skincare cream achieves the skin protection effect by inhibiting the degradation of collagen and the generation of free radicals induced by ultraviolet rays.

[0021] The above technical solutions of the present invention have the following beneficial technical effects:

[0022] 1. The anti-ultraviolet damage and anti-photoaging effects of the Chinese medicine skincare cream of the present invention are enhanced, and there are no toxic side effects and adverse reactions such as liver, kidney and heart damage after long-term application, and the effects are remarkable.

[0023] 2. The Cuscutae semen skincare cream of the present invention is superior to ordinary skincare creams. At the same time, for the preparation method of the Cuscutae semen skincare cream of the present invention, the content of active ingredients is relatively high, the product quality is more stable, and the effects are more remarkable. Brief Description of the Drawings

[0024] Figure 1 Process flow chart of Cuscutae Semen skin care cream according to an embodiment of the present invention;

[0025] Figure 2 Process flow chart of raw material mixing and extraction of Cuscutae Semen skin care cream according to an embodiment of the present invention;

[0026] Figure 3 Spectrum diagram of extract obtained by traditional water decoction extraction method according to an embodiment of the present invention;

[0027] Figure 4 Schematic diagram of spectrum of extract according to an embodiment of the present invention;

[0028] Figure 5 Experimental diagram of the effect of TSZ on the activity of human skin fibroblasts (HSF) according to an embodiment of the present invention. Detailed Description of the Invention

[0029] To make the objectives, technical solutions and advantages of the present invention clearer and more understandable, the present invention will be further described in detail below in conjunction with the specific embodiments and with reference to the accompanying drawings. It should be noted that, without conflict, the embodiments of the present invention and the features in the embodiments can be combined with each other.

[0030] It should be understood that these descriptions are only exemplary and are not intended to limit the scope of the present invention.

[0031] The following describes a preparation method and application of a Cuscutae Semen skin care cream provided by some embodiments of the present invention with reference to the accompanying drawings.

[0032] Combined with Figures 1-5 As shown, a preparation method of a Cuscutae Semen skin care cream provided by the present invention includes the following steps:

[0033] (1) Raw material treatment: Mix Cuscutae Semen, Rubi Fructus, and Nelumbinis Nuciferae Semen in a weight ratio of 1-10:1-5:1-5, then perform cleaning and weighing;

[0034] (2) Extraction: Soak the raw materials in a 30% ethanol solution in proportion, and then perform normal-temperature extraction 1-2 times under high-pressure conditions, and collect the extraction solution;

[0035] (3) Purification: Concentrate the extraction solution after high-speed centrifugation and three filtrations to obtain a traditional Chinese medicine extract;

[0036] (4) Emulsification: Mix the traditional Chinese medicine extract with 0.1-0.5% azone and orange peel essential oil, add purified water to form an aqueous phase, and emulsify with an oil phase component by stirring or homogenization, and then discharge after cooling;

[0037] (5) Inspection and Packaging: Inspect the semi-finished products and package them as finished products after passing the inspection.

[0038] This step combines high-pressure extraction with an emulsification process to ensure the efficient dissolution of active ingredients and the stability of the product.

[0039] Furthermore, in step (2), the pressure range of the high-pressure condition is 0.2 - 0.5 MPa, and the extraction time is 30 - 60 minutes per time. By limiting the high-pressure parameter range (0.2 - 0.5 MPa), it can not only promote the penetration of ethanol solution through the cell wall but also avoid the destruction of heat-sensitive components such as chlorogenic acid at high temperatures. The extraction time is optimized to 30 - 60 minutes to balance the extraction efficiency and energy consumption.

[0040] Furthermore, in step (3), the rotation speed of the high-speed centrifugation is 8000 - 12000 rpm, and the centrifugation time is 10 - 15 minutes. Macromolecular impurities and precipitates are removed through high-speed centrifugation (8000 - 12000 rpm), and three filtrations further ensure the purity of the extract and improve the stability of the subsequent emulsification process.

[0041] Furthermore, in step (4), the oil-phase components include vegetable oils and emulsifiers. The vegetable oils are selected from at least one of jojoba oil, olive oil, or shea butter. By using oil-phase components (natural vegetable oils), they have high compatibility with traditional Chinese medicine extracts, enhance the moisturizing property and skin feel of the skin care cream, and avoid the irritation of synthetic oils to sensitive skin.

[0042] Furthermore, the addition amount of orange peel essential oil is 0.1 - 0.3%, and the orange peel essential oil is extracted from citrus peels by steam distillation. The source (natural extraction) and addition amount (0.1 - 0.3%) of orange peel essential oil enhance the product aroma while exerting its synergistic penetration-promoting effect and enhancing the transdermal absorption of active ingredients.

[0043] Furthermore, in the Cuscutae chinensis skin care cream prepared by the above method, its active ingredient includes chlorogenic acid, the chlorogenic acid content ≥ 5 mg / g, and the ultraviolet absorption rate of the skin care cream ≥ 85%. By comparing with the traditional water decoction method in Example 1 (Table 1), it is proved that the extraction method of the present invention increases the chlorogenic acid content by more than 30%, and the ultraviolet absorption rate is significantly higher than that of ordinary sunscreen creams ( Figures 3-4 ).

[0044] Furthermore, in the Cuscutae chinensis skin care cream prepared by the above method, it is used for preventing or treating skin photoaging, and the skin care cream has been verified by toxicological experiments to have no liver, kidney, or heart toxicity and skin irritation.

[0045] Application of the dodder seed skin care cream in the preparation of sunscreen or anti-photoaging cosmetics. The skin care cream achieves skin protection by inhibiting ultraviolet-induced collagen degradation and free radical generation. Experiments have proven that it can reduce collagen loss (detected by Western blot) and scavenge free radicals (DPPH free radical scavenging rate ≥ 90%).

[0046] The dosage and usage of the traditional Chinese medicine skin care cream in Examples 1-3 of the present invention are as follows: After cleansing the face in the morning and evening every day, take 1-5 g of this product, knead it evenly in the palm, and then evenly apply it on the face and neck, and gently massage until completely absorbed. It can also be taken after bathing, and an appropriate amount of this product can be evenly applied to the whole body or the parts easily sunburned by the sun.

[0047] Experimental Example 1

[0048] The inventor prepared the dodder seed skin care cream according to the technological process of the dodder seed skin care cream in Example 1 of the present invention (as shown in Figure 2 ), and obtained the dodder seed traditional Chinese medicine skin care cream product. The mixed extraction technological process of dodder seeds, raspberry fruits, and lotus seeds in Example 1 (as shown in Figure 1 ), and obtained the extract.

[0049] The extracts obtained by the extraction method in Example 1 of the present invention and the extracts obtained by the traditional water decoction extraction method were compared. The extraction rate and extraction time are shown in Table 1. The effective component chlorogenic acid of the two methods was detected by HPLC, and the content of chlorogenic acid in the two extracts was detected by HPLC method. Figure 3 It is the chromatogram of the extract obtained by the traditional water decoction extraction method; Figure 4 It is the chromatogram of the extract obtained by the extraction method of Example 1. In the figure, A is chlorogenic acid.

[0050] Result: From Figure 4 , it can be seen that in this experiment, compared with other chromatographic peaks, the chromatographic peak of the effective component chlorogenic acid in the dodder seed skin care cream of the present invention is higher, indicating that not only the preparation process is feasible and reliable, but also the quality can be accurately controlled by establishing a quality index represented by the chlorogenic acid content.

[0051] The results show that the content of chlorogenic acid extracted by the present invention is 0.52%, far exceeding the traditional water decoction extraction method. The effective components of the traditional Chinese medicine raw materials are fully extracted, and the extraction time is greatly reduced. Not only the extraction rate is high, but also the extraction time is short and the effective components are high.

[0052] Table 1 Comparison of the effects of different extraction methods

[0053] Serial number Item Normal temperature and ultra-high pressure extraction method Traditional water decoction extraction method 1 Chlorogenic acid content in the extract 0.52％ 0.26％ 2 Extraction time 0.5-1.5h 2-4h

[0054] Example 2

[0055] A photodamage experimental model was established and anti-photoaging experiments were carried out. All samples restored HSF activity to varying degrees, but the compound dodder skin care cream of the present invention had a significant effect in restoring HSF activity, while other samples did not restore HSF activity significantly, indicating that the compound dodder skin care cream of the present invention has a strong ability to resist skin photoaging damage.

[0056] Figure 5 To investigate the effect of TSZ on the activity of human skin fibroblasts (HSF)

[0057] Results: From the top Figure 5 It can be seen that in this anti-photoaging experiment, all samples restored HSF activity to varying degrees, but the compound dodder skin care cream of the present invention had a significant effect in restoring HSF activity, while other samples did not restore HSF activity significantly, indicating that the compound dodder skin care cream of the present invention has a strong ability to resist skin photoaging damage.

[0058] Example 3

[0059] Student Peng, female, 26 years old, participated in a seaside cultural and tourism activity on a hot summer day, which caused sunburn on the face, redness and pain on the skin, and continued to use commercially available skin care cream for 1 week without improvement. Instead, the dodder skin care cream of the present invention was used. After taking 3g of this product and rubbing it in the palm, it was evenly applied to the affected part of the face. The facial skin felt good, and the redness and pain of the skin were significantly improved until the third day. After continued use, the skin was basically normal on the sixth day. There was no discomfort related to organs such as liver, kidney, and heart.

[0060] The above usage examples prove that the traditional Chinese medicine skin care cream of the present invention has enhanced effects in preventing ultraviolet damage and photoaging, and has no toxic side effects and adverse reactions such as liver, kidney and heart damage when used for a long time, and has significant efficacy. The dodder skin care cream of the present invention is superior to ordinary skin care creams. At the same time, the preparation method of the dodder skin care cream of the present invention has a higher content of effective ingredients, more stable product quality, and more significant efficacy.

[0061] The preparation method of the invention is suitable for large-scale production, and the obtained skin care cream can be widely used in the field of cosmetics such as sunscreen cream, anti-aging lotion, etc., and has significant market prospects.

[0062] Although the embodiments of the present invention have been disclosed as above, they are not limited to the applications listed in the specification and the implementation modes. They can be fully applied to various fields suitable for the present invention. For those familiar with the art, additional modifications can be easily implemented. Therefore, without departing from the general concept defined by the claims and the scope of equivalents, the present invention is not limited to the specific details and the embodiments shown and described herein.

Claims

1. A method for preparing a dodder skin care cream, characterized in that: The following steps are involved: (1) Raw material processing: mix dodder seeds, raspberries, and lotus seeds in a weight ratio of 1-10:1-5:1-5, and then clean and weigh; (2) Extraction: Soak the raw material in a 30% ethanol solution in a certain proportion, extract it 1-2 times at room temperature under high pressure conditions, and collect the extract; (3) Purification: The extract is concentrated by high-speed centrifugation and three-time filtration to obtain the Chinese medicine extract; (4) Emulsification: mixing the Chinese herbal extract with 0.1-0.5% of azone and orange peel essential oil, adding purified water to form an aqueous phase, stirring or homogenizing with the oil phase components to emulsify, and discharging after cooling; (5) Inspection and packaging: Semi-finished products are inspected and packaged as finished products after they pass the inspection.

2. The method for preparing a Cuscuta skin care cream according to claim 1, characterized in that: The pressure range of the high pressure condition in step (2) is 0.2-0.5 MPa, and the extraction time is 30-60 minutes / time.

3. The method for preparing a Cuscuta skin care cream according to claim 1, characterized in that: The rotation speed of the high-speed centrifugation in step (3) is 8000-12000 rpm, and the centrifugation time is 10-15 minutes.

4. The method for preparing a Cuscuta skin care cream according to claim 1, characterized in that: The oil phase components in step (4) include vegetable oil and emulsifier, and the vegetable oil is selected from at least one of jojoba oil, olive oil or shea butter.

5. The method for preparing a Cuscuta skin care cream according to claim 1, characterized in that: The addition amount of the orange peel essential oil is 0.1-0.3%, and the orange peel essential oil is extracted from citrus peels by steam distillation.

6. A dodder skin care cream prepared according to the method according to any one of claims 1 to 5, characterized in that: Its effective ingredients include chlorogenic acid, the content of the chlorogenic acid is ≥5mg / g, and the ultraviolet absorption rate of the skin care cream is ≥85%.

7. The dodder skin care cream according to claim 6, characterized in that: The invention is used for preventing or treating skin photoaging, and the skin care cream is verified by toxicology experiments to have no liver, kidney and heart toxicity and no skin irritation.

8. Use of the dodder skin care cream according to claim 6 or 7 in the preparation of sunscreen or anti-photoaging cosmetics, characterized in that: The skin care cream achieves skin protection effect by inhibiting collagen degradation and free radical generation induced by ultraviolet rays.

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