Application of microbial composition in industrial hazardous waste treatment

Abstract

The invention discloses application of a microbial composition in industrial hazardous waste treatment, and belongs to the technical field of industrial hazardous waste treatment.The application comprises the following steps that S1, industrial hazardous waste is collected, the industrial hazardous waste is pretreated to obtain a hazardous waste material, and a fermentation tank is filled with the hazardous waste material; s2, adding a growth culture medium into a bioreactor, inoculating the domesticated bacterial liquid into the bioreactor, and carrying out enlarged culture to obtain a fermentation material of the composite acidophilic thiobacillus; s3, adding a biosurfactant into the fermentation material of the composite acidophilic thiobacillus, and uniformly stirring to obtain the composite acidophilic thiobacillus with hydrophobically modified cell membrane surface; and S4, adding an acidic pH buffer solution to obtain a to-be-treated material. According to the method, in the industrial hazardous waste treatment process, the stability of the pH required by the acidithiobacillus acidophilus can be maintained, and the cell membrane surface hydrophobicity of the composite acidithiobacillus acidophilus is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of industrial hazardous waste treatment, and specifically to the application of a microbial composition in industrial hazardous waste treatment. Background Art

[0002] The ultimate goal of industrial hazardous waste treatment is harmlessness, reduction, and resource utilization. Currently, methods for industrial hazardous waste treatment include landfill treatment, incineration treatment, pulverization treatment, biological treatment, and various chemical methods, etc. Among them, biological treatment mainly uses microorganisms to decompose industrial hazardous waste. Due to its low cost, environmental friendliness, and no secondary pollution, it has always attracted the attention of many researchers. During the production and manufacturing processes in industries such as medicine, chemical engineering, and new materials, due to the complex composition of the generated waste, it often contains complex organic compounds and many substances that are difficult to biodegrade, such as residues at the bottom of various reaction kettles, rectification residues after solvent recovery, and waste activated carbon used for adsorption and separation, etc. The treatment of these hazardous wastes not only has great technical difficulties but also high costs, bringing heavy economic and environmental pressures to relevant enterprises.

[0003] Heavy metal-containing hazardous wastes such as waste batteries, fly ash from municipal solid waste incineration, and spent catalysts are all highly alkaline solid oxides or hydroxides. The valuable metals in heavy metal-containing hazardous wastes will strongly consume H+ during the bioleaching process, resulting in a high pH value of the leachate, which endangers the growth and activity of acidophilic autotrophic strains in the leachate. It can be seen that under high solid-liquid ratio conditions, the pH value of the leachate is higher and the growth and activity of acidophilic autotrophic strains are lower. The bioleaching technology has a significantly reduced treatment capacity for heavy metal-containing hazardous wastes and a significantly increased treatment cost under high solid-liquid ratio, so the leaching efficiency is low. At the same time, highly toxic organic or inorganic substances in heavy metal-containing hazardous wastes, such as high-concentration fluoride ions, lithium hexafluorophosphate, etc., will all endanger the growth of working strains and may ultimately lead to the stop of the bioleaching process. Due to the existence of these factors, the bioleaching technology has a significantly reduced treatment capacity for heavy metal-containing hazardous wastes and a significantly increased treatment cost under high solid-liquid ratio, which has become a major obstacle to the practical application of this technology. Summary of the Invention

[0004] In order to overcome the deficiencies of the prior art, one of the purposes of the present invention is to provide the application of a microbial composition in industrial hazardous waste treatment, which can maintain the pH stability required by Acidithiobacillus during the treatment of industrial hazardous waste and improve the cell membrane surface hydrophobicity of composite Acidithiobacillus.

[0005] One of the purposes of the present invention is achieved by adopting the following technical solutions: The application of a microbial composition in industrial hazardous waste treatment includes the following steps: S1. Collect industrial hazardous waste, pre-treat the industrial hazardous waste to obtain hazardous waste materials; fill the hazardous waste materials in a fermentation tank; S2. Acclimatize the composite Acidithiobacillus to the pH in the culture medium to obtain cells that can still maintain sulfur oxidation activity under the condition of pH 3.5 - 5.0. Add a growth medium to the bioreactor. The growth medium includes inorganic salts and a sulfur source. Use FeSO4·7H2O to lower the pH of the growth medium to 2.0 - 3.0. Inoculate the acclimatized bacterial solution into the bioreactor for scale-up culture, and culture it for 4 - 5 days under the stirring conditions of a temperature of 30 - 50°C and 120 - 170 rpm. The composite Acidithiobacillus enters the logarithmic growth phase, and the pH drops below 2.0 to obtain the fermentation material of the composite Acidithiobacillus. S3. Add a biosurfactant to the fermentation material of the composite Acidithiobacillus, and stir evenly to obtain the composite Acidithiobacillus with hydrophobic modification on the cell membrane surface. S4. Dilute the hazardous waste material with distilled water to a solid concentration of 10% by mass fraction, and then add an acidic pH buffer solution to obtain the material to be treated. S5. Inoculate the bacterial solution of the composite Acidithiobacillus with hydrophobic modification on the cell membrane surface obtained in step S3 into the fermentation tank, and then supplement and add the sulfur source as an energy substance for cultivation. Mix evenly quickly in the fermentation tank to enable the material to be treated to come into full and uniform contact with the composite Acidithiobacillus with hydrophobic modification on the cell membrane surface and ferment. After fermentation, the industrial hazardous waste is effectively treated. Further, the sulfur source includes elemental sulfur or sulfide ore, and the sulfide ore includes at least one of pyrite, chalcopyrite, and arsenopyrite.

[0006] Further, in step S2, the composite Acidithiobacillus is a mixed flora of Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans, and Leptospirillum ferrooxidans.

[0007] Further, in step S2, the inorganic salt components of the growth medium include, by mass concentration: 0.5 - 2.0 g / L of KNO3, 0.5 - 2.0 g / L of NaCl, 0.5 - 2.0 g / L of MgSO4·7H2O, 0.25 - 1.0 g / L of CaCl2, and the balance is water.

[0008] Further, in step S2, the acidic pH buffer solution is a potassium chloride-hydrochloric acid buffer solution, and the pH buffering range is 1.0 - 2.2.

[0009] Further, according to the amount of 100 mL, the preparation steps of the potassium chloride - hydrochloric acid buffer solution are as follows: Mix 37.64 mL of 0.1 mol / L HCl and 31.02 mL of 0.02 mol / L KCl solution and then make up the volume to 100 mL.

[0010] Further, in step S5, the inoculation concentration is 5% - 20% (v / v), the temperature of the fermentation treatment is 25 to 35 °C, and the treatment time is 30 h.

[0011] Further, in step S3, the preparation method of the biosurfactant is as follows: Prepare the growth medium and fermentation medium of Pseudomonas aeruginosa (Pseudomonas aeruginosa ATCC 9027), inoculate the Pseudomonas aeruginosa into the growth medium to obtain a Pseudomonas aeruginosa culture, inoculate the Pseudomonas aeruginosa culture into the fermentation medium to produce the biosurfactant, and then separate and purify it.

[0012] Further, in step S1, the pretreatment method of the industrial hazardous waste is one of direct crushing, crushing after separation, and drying and crushing after separation.

[0013] Compared with the prior art, the beneficial effects of the present invention are as follows: The present invention provides the application of a microbial composition in the treatment of industrial hazardous waste. In step S4, adding an acidic pH buffer solution can maintain the pH stability required by Acidithiobacillus ferrooxidans during the treatment of industrial hazardous waste, keep the growth and activity of Acidithiobacillus ferrooxidans, and the inorganic salts in the culture medium are acidic salts. After Acidithiobacillus ferrooxidans utilizes the sulfur source, hydrogen ions will also be generated to neutralize the highly alkaline oxides or hydroxides contained in the hazardous waste material and maintain an acidic environment; in step S3, adding the biosurfactant to the fermentation material of the composite Acidithiobacillus ferrooxidans and stirring evenly to obtain the composite Acidithiobacillus ferrooxidans with hydrophobic modification on the cell membrane surface. The biosurfactant can improve the hydrophobic modification of the cell membrane surface of the composite Acidithiobacillus ferrooxidans. The cell membrane surface has good hydrophobicity, the cell membrane can maintain stability in an acidic environment, is not easily disintegrated, and is conducive to the adsorption of Acidithiobacillus ferrooxidans on the hazardous waste material, improving the degradation ability of Acidithiobacillus ferrooxidans to the hazardous waste material. Specific embodiments

[0014] The following further describes the present invention in combination with specific embodiments. It should be noted that on the premise of no conflict, the following described embodiments or technical features can be arbitrarily combined to form new embodiments.

[0015] Pseudomonas aeruginosa (Pseudomonas aeruginosa ATCC 9027) was purchased from the Guangdong Institute of Microbiology. The culture was maintained at 4 °C in a test tube with solid nutrient agar.

[0016] Acid adaptation mechanism of acidophilic bacteria Acidophilic bacteria grow in acidic environments, and the cell membrane is the only physical barrier that can resist external H+. Therefore, the low permeability of the cell membrane to H+ may be the main way to maintain the internal pH of the cell. Research in recent years has shown that lipid substances on the cell membrane play an important role in this regard. It has been found that liposomes extracted from the cell membrane of this acidophilic bacterium cannot form regular vesicle structures at pH 7.0, but can form such regular structures at pH 3.0 and 4.0, and the permeability of this structure to H+ is extremely low. However, the liposomes of this structure will become leaky at pH 7.0. Therefore, acidophilic bacteria not only well adapt to acidic environments, but also require acidic environments to ensure the stability of their cell membranes and the integrity of the cells.

[0017] Most acidophilic bacteria are chemolithoautotrophs. For example, extreme acidophilic bacteria include Sulfolobus and Thermoplasma acidophilum belonging to archaea, Thiobacillus thiooxidans and Acidithiobacillus ferrooxidans belonging to eubacteria. Thermoplasma acidophilum and Sulfolobus are extreme thermoacidophilic archaea, distributed in coal piles and acidic hot springs. Their optimal growth pH values are 0.8 - 3 and 1 - 5.5 respectively. The optimal growth pH values of Thiobacillus thiooxidans and Acidithiobacillus ferrooxidans are 2 - 5 and 1.5 - 4 respectively, and they often appear in acidic wastewater containing sulfuric acid, coal mines, acid mine waters or acidic springs. Thiobacillus thiooxidans and Acidithiobacillus ferrooxidans are Gram-negative, aerobic chemolithoautotrophs. The ATP and [H] required for CO2 reduction are obtained by oxidizing elemental sulfur to sulfuric acid, and at the same time, the environmental pH value drops to 1 - 1.5. However, the internal pH value of their bodies is 6 - 6.5, and the optimal pH value of their ATPase is 7.5 - 7.8. In addition, Thiobacillus thiooxidans cannot oxidize Fe 2+ , Acidithiobacillus ferrooxidans can oxidize Fe 2+ . Example 1

[0018] This example provides the application of a microbial composition in the treatment of industrial hazardous waste, including the following steps: S1. Collect industrial hazardous waste, and obtain hazardous waste materials after crushing and pretreatment of the industrial hazardous waste; fill the hazardous waste materials in a fermentation tank; S2. Acclimatize the composite Acidithiobacillus to the pH in the culture medium to obtain cells that can still maintain sulfur oxidation activity at a pH of 3.5. Add a growth medium to the bioreactor. The growth medium includes inorganic salts and a sulfur source. Use FeSO4·7H2O to lower the pH of the growth medium to 2.0. Inoculate the acclimatized bacterial solution into the bioreactor for scale-up cultivation, and cultivate it under stirring conditions at a temperature of 30 °C and 120 rpm for 4 days. The composite Acidithiobacillus enters the logarithmic growth phase, and the pH drops below 2.0 to obtain the fermentation material of the composite Acidithiobacillus. S3. Add a biosurfactant to the fermentation material of the composite Acidithiobacillus and stir evenly to obtain the composite Acidithiobacillus with hydrophobic modification on the cell membrane surface. S4. Dilute the hazardous waste material with distilled water to a solid concentration of 10% by mass fraction, and then add an acidic pH buffer solution to obtain the material to be treated. S5. Inoculate the bacterial solution of the composite Acidithiobacillus with hydrophobic modification on the cell membrane surface obtained in step S3 into the fermentation tank at an inoculation concentration of 5% (v / v), and then supplement and add a sulfur source as an energy substance for cultivation. Mix evenly quickly in the fermentation tank to enable the material to be treated to come into full and even contact with the composite Acidithiobacillus with hydrophobic modification on the cell membrane surface and ferment. The temperature of the fermentation treatment is 25 °C, and the treatment time is 30 h. After the fermentation is completed, the industrial hazardous waste is effectively treated.

[0019] The sulfur source in this example can be elemental sulfur or sulfide ore, and the sulfide ore includes at least one of pyrite, chalcopyrite, and arsenopyrite.

[0020] The composite Acidithiobacillus is a mixed flora of Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans, and Leptospirillum ferrooxidans.

[0021] The inorganic salt components of the growth medium include, by mass concentration: 0.5 - 2.0 g / L of KNO3, 0.5 - 2.0 g / L of NaCl, 0.5 - 2.0 g / L of MgSO4·7H2O, 0.25 - 1.0 g / L of CaCl2, and the balance is water.

[0022] The acidic pH buffer solution is a potassium chloride-hydrochloric acid buffer solution, and the pH buffering range is 1.0 - 2.2. According to a quantity of 100 mL, the preparation steps of the potassium chloride-hydrochloric acid buffer solution are as follows: Mix 37.64 mL of 0.1 mol / L HCl and 31.02 mL of 0.02 mol / L KCl solution and then make up the volume to 100 mL.

[0023] Furthermore, the preparation method of the biosurfactant is as follows: Prepare the growth medium and fermentation medium for Pseudomonas aeruginosa (Pseudomonas aeruginosa ATCC 9027), inoculate Pseudomonas aeruginosa into the growth medium to obtain a Pseudomonas aeruginosa culture, and inoculate the Pseudomonas aeruginosa culture into the fermentation medium to produce the biosurfactant, which can be obtained through separation and purification.

[0024] Among them, the specific preparation steps of the biosurfactant are as follows: 1. Preparation of the growth medium: By mass percentage, 0.1% beef extract, 0.2% yeast extract, 0.5% heptanone, and 0.5% sodium chloride, with a pH value of 7.0. The growth parameters are a temperature of 28 degrees Celsius, stirring at 150 revolutions per minute for 16 hours until an optical density (OD) of 0.7 is obtained at 600 nanometers (equivalent to 107 CFUs / mL).

[0025] 2. Preparation of the fermentation medium By mass percentage, 5.0% glycerol, 2.0% glucose, 0.1% K2HPO4, 0.1% K2HPO4, 0.02% MgSO 4. 7H2O, 0.02% CaCl 2. H2O, 0.005% FeCl3·6H2O, with a pH value of 7.0. The production parameters are a temperature of 28 - 35 °C and stirring at 200 revolutions per minute for 96 hours.

[0026] 3. Separation and purification of the biosurfactant Add ethyl acetate (C4H8O2) with a volume ratio of 1:1 to the fermented broth to extract the biosurfactant. Centrifuge the mixture at 5000 rpm for 10 minutes, stir vigorously for 15 minutes, and let it stand until phase separation. The organic phase is removed, and the organic phase is evaporated in a rotary evaporator at 40 °C to obtain a viscous yellow product, which is treated with NaOH and washed with acetone to remove impurities and dirt to the maximum extent. The yield of the separated biosurfactant is expressed in grams per liter. Example 2

[0027] This example provides the application of the microbial composition in the treatment of industrial hazardous waste, including the following steps: S1. Collect industrial hazardous waste, separate the industrial hazardous waste according to the type of waste, and crush it after separation to obtain hazardous waste materials; fill the hazardous waste materials in a fermentation tank; S2. Acclimatize the composite Acidithiobacillus to the pH in the culture medium to obtain cells that can still maintain sulfur oxidation activity under the condition of pH 3.5 - 5.0. Add the growth medium to the bioreactor. The growth medium includes inorganic salts and a sulfur source. Use FeSO₄·7H₂O to lower the pH of the growth medium to 3.0. Inoculate the acclimatized bacterial solution into the bioreactor for scale-up culture, and culture it for 5 days under the stirring condition of 40°C and 150 rpm. The composite Acidithiobacillus enters the logarithmic growth phase, and the pH drops below 2.0 to obtain the fermentation material of the composite Acidithiobacillus. S3. Add the biosurfactant to the fermentation material of the composite Acidithiobacillus and stir evenly to obtain the composite Acidithiobacillus with hydrophobic modification on the cell membrane surface. S4. Dilute the hazardous waste material with distilled water to a solid concentration of 10% by mass fraction, and then add an acidic pH buffer solution to obtain the material to be treated. S5. Inoculate the bacterial solution of the composite Acidithiobacillus with hydrophobic modification on the cell membrane surface obtained in step S3 into the fermentation tank at an inoculation concentration of 10% (v / v), then supplement and add a sulfur source as an energy substance for cultivation, and mix evenly quickly in the fermentation tank to enable the material to be treated to come into full and uniform contact with the composite Acidithiobacillus with hydrophobic modification on the cell membrane surface and ferment. The temperature for fermentation treatment is 30°C, and the treatment time is 30 h. After fermentation, the industrial hazardous waste is effectively treated.

[0028] The sulfur source in this example can be elemental sulfur or sulfide ore, and the sulfide ore includes at least one of pyrite, chalcopyrite, and arsenopyrite.

[0029] The composite Acidithiobacillus is a mixed flora of Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans, and Leptospirillum ferrooxidans.

[0030] The inorganic salt components of the growth medium include, by mass concentration: 0.5 - 2.0 g / L of KNO₃, 0.5 - 2.0 g / L of NaCl, 0.5 - 2.0 g / L of MgSO₄·7H₂O, 0.25 - 1.0 g / L of CaCl₂, and the balance is water.

[0031] The acidic pH buffer solution is a potassium chloride-hydrochloric acid buffer solution, and the pH buffering range is 1.0 - 2.2. According to the amount of 100 mL, the preparation steps of the potassium chloride-hydrochloric acid buffer solution are as follows: Mix 37.64 mL of 0.1 mol / L HCl and 31.02 mL of 0.02 mol / L KCl solution and then make up the volume to 100 mL.

[0032] Furthermore, the preparation method of the biosurfactant is as follows: Prepare the growth medium and fermentation medium for Pseudomonas aeruginosa (Pseudomonas aeruginosa ATCC 9027), inoculate Pseudomonas aeruginosa into the growth medium to obtain a Pseudomonas aeruginosa culture, inoculate the Pseudomonas aeruginosa culture into the fermentation medium to produce the biosurfactant, and then separate and purify it. Example 3

[0033] This example provides the application of a microbial composition in the treatment of industrial hazardous waste, including the following steps: S1. Collect industrial hazardous waste, separate the industrial hazardous waste according to the type of waste, and after separation, dry it and then crush it to obtain hazardous waste materials; fill the hazardous waste materials in a fermentation tank; S2. Acclimatize the composite Acidithiobacillus thiooxidans in a medium to obtain cells that can still maintain sulfur oxidation activity under the condition of pH 5.0; add a growth medium to a bioreactor, the growth medium includes inorganic salts and a sulfur source, use FeSO4·7H2O to lower the pH of the growth medium to 2.5, inoculate the acclimatized bacterial solution into the bioreactor for scale-up culture, and culture it for 4 - 5 days under the stirring conditions of a temperature of 50°C and 170 rpm. The composite Acidithiobacillus thiooxidans enters the logarithmic growth phase and the pH drops below 2.0 to obtain the fermentation material of the composite Acidithiobacillus thiooxidans; S3. Add the biosurfactant to the fermentation material of the composite Acidithiobacillus thiooxidans and stir evenly to obtain the composite Acidithiobacillus thiooxidans with hydrophobic modification on the cell membrane surface; S4. Dilute the hazardous waste materials with distilled water to a solid concentration of 10% by mass fraction, and then add an acidic pH buffer solution to obtain the material to be treated; S5. Inoculate the bacterial solution of the composite Acidithiobacillus thiooxidans with hydrophobic modification on the cell membrane surface obtained in step S3 into the fermentation tank at an inoculation concentration of 20% (v / v), then supplement and add a sulfur source as an energy substance for cultivation, and quickly mix evenly in the fermentation tank so that the material to be treated can be in full and uniform contact with the composite Acidithiobacillus thiooxidans with hydrophobic modification on the cell membrane surface and ferment. The temperature of the fermentation treatment is 35°C and the treatment time is 30 h. After the fermentation is completed, the industrial hazardous waste is effectively treated.

[0034] The sulfur source in this example can be elemental sulfur or sulfide ore, and the sulfide ore includes at least one of pyrite, chalcopyrite, and arsenopyrite.

[0035] The composite Acidithiobacillus is a mixed flora of Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans, and Leptospirillum ferrooxidans.

[0036] The inorganic salt components of the growth medium include, by mass concentration: 0.5 - 2.0 g / L of KNO3, 0.5 - 2.0 g / L of NaCl, 0.5 - 2.0 g / L of MgSO4·7H2O, 0.25 - 1.0 g / L of CaCl2, and the balance is water.

[0037] The acidic pH buffer solution is a potassium chloride - hydrochloric acid buffer solution with a pH buffering range of 1.0 - 2.2. For a 100 mL amount, the preparation steps of the potassium chloride - hydrochloric acid buffer solution are as follows: Mix 37.64 mL of 0.1 mol / L HCl and 31.02 mL of 0.02 mol / L KCl solution and then make up the volume to 100 mL.

[0038] Furthermore, the preparation method of the biosurfactant is as follows: Prepare the growth medium and fermentation medium for Pseudomonas aeruginosa (Pseudomonas aeruginosa ATCC 9027), inoculate Pseudomonas aeruginosa into the growth medium to obtain a Pseudomonas aeruginosa culture, inoculate the Pseudomonas aeruginosa culture into the fermentation medium to produce the biosurfactant, and then separate and purify it.

[0039] The above - mentioned embodiments are only the preferred embodiments of the present invention and cannot be used to limit the scope of protection of the present invention. Any non - substantial changes and substitutions made by those skilled in the art based on the present invention belong to the scope of protection required by the present invention.

Claims

1. Application of a microbial composition in the treatment of industrial hazardous waste, characterized in that: The following steps are involved: S1. Collecting industrial hazardous waste, pre-treating the industrial hazardous waste to obtain hazardous waste materials; and filling the hazardous waste materials into a fermentation tank; S2, performing pH adaptive domestication of the composite acidophilic thiobacillus in a culture medium to obtain a bacterial cell that can still maintain sulfur oxidation activity under a pH condition of 3.5 to 5.0; adding a growth medium into a bioreactor, wherein the growth medium comprises an inorganic salt and a sulfur source, and reducing the pH of the growth medium to 2.0 to 3.0 with FeSO4·7H2O, connecting the domesticated bacterial liquid to the bioreactor for expansion culture, and cultivating for 4 to 5 days at a temperature of 30 to 50° C. and a stirring condition of 120 to 170 rpm, wherein the composite acidophilic thiobacillus enters a logarithmic growth phase, and the pH drops to below 2.0, thereby obtaining a fermentation material of the composite acidophilic thiobacillus; S3, adding a biosurfactant to the fermentation material of the composite acidophilic thiobacillus, stirring evenly, to obtain a composite acidophilic thiobacillus with hydrophobic modification on the cell membrane surface; S4, diluting the hazardous waste material with distilled water to a solid concentration of 10% by mass, and then adding an acidic pH buffer solution to obtain a material to be treated; S5, inoculating the bacterial liquid of the composite acidophilic thiobacillus with hydrophobic modification on the cell membrane surface obtained in step S3 into the fermentation tank, and then supplementing the sulfur source as an energy material for cultivation, and quickly mixing and evenly mixing in the fermentation tank, so that the material to be processed can be fully and evenly contacted with the composite acidophilic thiobacillus with hydrophobic modification on the cell membrane surface and fermented, and after the fermentation is completed, the industrial hazardous waste is effectively treated.

2. The use of the microbial composition according to claim 1 in the treatment of industrial hazardous waste, characterized in that: In step S2, the sulfur source includes elemental sulfur or sulfide ore, and the sulfide ore includes at least one of pyrite, chalcopyrite, and arsenopyrite.

3. The use of the microbial composition according to claim 1 in the treatment of industrial hazardous waste, characterized in that: In step S2, the composite acidophilic thiobacillus is a mixed bacterial flora of Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans, and Leptospirillumferrooxidans.

4. The use of the microbial composition according to claim 1 in the treatment of industrial hazardous waste, characterized in that: In step S2, the inorganic salt components of the growth medium include, by mass concentration, 0.5-2.0 g / L of KNO3, 0.5-2.0 g / L of NaCl, 0.5-2.0 g / L of MgSO4.7H2O, 0.25-1.0 g / L of CaCl2, and the remainder is water.

5. The use of the microbial composition according to claim 1 in the treatment of industrial hazardous waste, characterized in that: In step S4, the acidic pH buffer solution is a potassium chloride-hydrochloric acid buffer solution, and the pH buffer range is 1.0-2.

2.

6. Use of the microbial composition according to claim 5 in the treatment of industrial hazardous waste, characterized in that: According to the amount of 100 mL, the preparation steps of the potassium chloride hydrochloric acid buffer solution are as follows: 37.64 mL of 0.1 mol / L HCl and 31.02 mL of 0.02 mol / L KCL solution are mixed and then the volume is adjusted to 100 mL.

7. The use of the microbial composition according to claim 1 in the treatment of industrial hazardous waste, characterized in that: In step S5, the inoculation concentration is 5%-20% (v / v), the fermentation temperature is 25-35° C., and the treatment time is 30 h.

8. The use of the microbial composition according to claim 1 in the treatment of industrial hazardous waste, characterized in that: In step S3, the preparation method of the biosurfactant is: preparing a growth medium and a fermentation medium of Pseudomonas aeruginosa (Pseudomonas aeruginosa ATCC 9027), inoculating the Pseudomonas aeruginosa into the growth medium to obtain a Pseudomonas aeruginosa culture, inoculating the Pseudomonas aeruginosa culture into the fermentation medium to produce the biosurfactant, and then separating and purifying it.

9. The use of the microbial composition according to claim 1 in the treatment of industrial hazardous waste, characterized in that: In step S1, the industrial hazardous waste is pretreated by direct crushing, crushing after separation, or drying and crushing after separation.