Sampling equipment, sampling method, controller, sampling control system and cell processing device

Abstract

The invention discloses sampling equipment, a sampling method, a controller, a sampling control system and a cell processing device. The equipment comprises a sampling container, an air pump, a first pipeline and a second pipeline, the first pipeline is used for connecting the centrifugal container and the sampling container; a first one-way valve is arranged on the first pipeline; the second pipeline is used for connecting the sampling container and the cell counting equipment; a second one-way valve is arranged on the second pipeline; the air pump is connected with the sampling container, and when the air pump is in an air exhaust state, the first one-way valve is opened and the second one-way valve is closed, so that to-be-sampled liquid flows into the sampling container from the centrifugal container; when the air pump is in an air blowing state, the first one-way valve is closed and the second one-way valve is opened, so that the to-be-sampled liquid flows into the cell counting equipment. According to the device, by controlling the state of the air pump in the sampling device, sampling of the to-be-sampled liquid is accurate, when the device is applied to the sampling process, sampling can be achieved without manual intervention and frequent opening, operation is easy and convenient, and sterile sampling can be achieved.

Description

Technical Field

[0001] The present invention relates to the field of cell processing, and in particular to a sampling device, method, controller, sampling control system and cell processing device. Background Art

[0002] During the production process of cell-based drugs, it is necessary to regularly sample cells to monitor indicators such as cell viability, loss rate and density. Therefore, it is necessary to perform aseptic sampling on cells. However, the existing aseptic sampling methods generally involve moving the sampling device into a sterile environment and opening the lid of the centrifuge container for sampling. This sampling method requires manual operation, the operation process is cumbersome and the sampling volume cannot be accurately controlled. Moreover, frequently opening the lid of the centrifuge container is likely to cause cell contamination.

[0003] Therefore, how to achieve relatively accurate aseptic sampling is a technical problem that needs to be solved urgently at present. Summary of the Invention

[0004] Embodiments of the present invention provide a sampling device, method, controller, sampling control system and cell processing device to solve the technical problem of how to perform relatively accurate aseptic sampling.

[0005] A sampling device includes a sampling container, an air pump, a first pipeline and a second pipeline;

[0006] The first pipeline is used to connect the centrifuge container and the sampling container, and a first one-way valve is provided on the first pipeline;

[0007] The second pipeline is used to connect the sampling container and the cell counting device, and a second one-way valve is provided on the second pipeline;

[0008] The air pump is connected to the sampling container. When the air pump is in the air extraction state, the first one-way valve is opened and the second one-way valve is closed, so that the liquid to be sampled flows from the centrifuge container into the sampling container through the first pipeline; when the air pump is in the air blowing state, the first one-way valve is closed and the second one-way valve is opened, so that the liquid to be sampled flows from the sampling container into the cell counting device through the second pipeline.

[0009] Preferably, the sampling device further includes a monitoring and control module, which is connected to the air pump and is used to control the air pump to switch between the air extraction state and the air blowing state according to the current monitoring data;

[0010] Further, the monitoring and control module includes an induction unit and a control unit;

[0011] The induction unit is arranged on the sampling container and is used to collect the current monitoring data of the liquid to be sampled in the sampling container;

[0012] The control unit, connected to the induction unit and the air pump, is configured to control the air pump to switch between the air extraction state and the air blowing state according to the current monitoring data.

[0013] Preferably, the induction unit includes a signal induction source and a signal inductor, which are respectively arranged on both sides of the sampling container for collecting the current liquid volume of the liquid to be sampled in the sampling container.

[0014] The control unit, connected to the signal inductor, is configured to control the air pump to be in the air blowing state when the current liquid volume is greater than the preset liquid volume; and control the air pump to be in the air extraction state when the current liquid volume is not greater than the preset liquid volume.

[0015] Preferably, the induction unit includes a pressure sensor arranged in the sampling container for collecting the current liquid pressure of the liquid to be sampled in the sampling container.

[0016] The control unit, connected to the pressure sensor, is configured to control the air pump to be in the air blowing state when the current liquid pressure is greater than the preset liquid pressure; and control the air pump to be in the air extraction state when the current liquid pressure is not greater than the preset liquid pressure.

[0017] A sampling method applicable to the above sampling device, the sampling method includes:

[0018] In response to the received sampling instruction, control the air pump to be in the air extraction state, so that the first one-way valve opens and the second one-way valve closes, enabling the liquid to be sampled to flow from the centrifuge container into the sampling container through the first pipeline.

[0019] Obtain the current monitoring data, and when the current monitoring data meets the preset switching condition, control the air pump to be in the air blowing state, so that the first one-way valve closes and the second one-way valve opens, enabling the liquid to be sampled to flow from the sampling container into the cell counting device through the second pipeline.

[0020] Preferably, the current monitoring data is the current air extraction duration.

[0021] Preferably, the step of controlling the air pump to be in the air blowing state when the current monitoring data meets the preset switching condition includes:

[0022] If the current air extraction duration reaches the preset duration, control the air pump to be in the air blowing state.

[0023] If the current air extraction duration does not reach the preset duration, control the air pump to be in the air extraction state.

[0024] Preferably, the current monitoring data is the current liquid volume of the liquid to be sampled in the sampling container;

[0025] Preferably, when the current monitoring data meets a preset switching condition, controlling the air pump to be in a blowing state includes:

[0026] If the current liquid volume reaches a preset liquid volume, then control the air pump to be in a blowing state;

[0027] If the current liquid volume does not reach the preset liquid volume, then control the air pump to be in a pumping state.

[0028] A sampling controller includes a memory, a processor, and a computer program stored in the memory and executable on the processor. When the processor executes the computer program, the above-mentioned sampling method is implemented.

[0029] A sampling control system includes the above-mentioned sampling device, the above-mentioned sampling controller, and a centrifuge container. The sampling controller is respectively connected to the sampling device and the centrifuge container for implementing the above-mentioned sampling method.

[0030] A cell processing device includes the above-mentioned sampling control system and a cell counting device;

[0031] The centrifuge container is provided with air permeable holes, and an air permeable membrane is arranged on the air permeable holes for enabling the centrifuge container to obtain sterile air;

[0032] The sampling controller is connected to the centrifuge container for controlling the centrifuge container inlet and the centrifuge container outlet to be closed and controlling the centrifuge container to continue to be in a working state after receiving a sampling instruction;

[0033] The cell counting device is used for counting the cells in the liquid to be sampled flowing into the cell counting device.

[0034] The above-mentioned sampling device, method, controller, sampling control system, and cell processing device. By controlling the blowing state and the pumping state of the air pump in the sampling device, the sampling of the liquid to be sampled can be made more accurate. Moreover, when this sampling device is applied to the sampling process, it can achieve the sampling purpose without manual intervention and without frequent opening. The operation is simple and convenient, and sterile sampling can be achieved. This method does not require manual participation in sampling and does not require frequent opening of the sampling device. Only by switching the air pump between the pumping state and the blowing state, the automatic sampling of the liquid to be sampled and the output of the sample can be realized, which can ensure the sterile environment of the sampling device and ensure sterile sampling. And by obtaining the current monitoring data to control the switching of the air pump between the pumping state and the blowing state, more accurate sampling is achieved. Description of the Drawings

[0035] To more clearly illustrate the technical solutions of the embodiments of the present invention, the following will briefly introduce the drawings required for the description of the embodiments of the present invention. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0036] Figure 1 It is a connection relationship diagram of a sampling device and a centrifuge container in an embodiment of the present invention;

[0037] Figure 2 It is a flowchart of a sampling method in an embodiment of the present invention;

[0038] Figure 3 is Figure 2 a flowchart of step S202 in;

[0039] Figure 4 is Figure 2 another flowchart of step S202 in;

[0040] Figure 5 It is a schematic diagram of a sampling controller in an embodiment of the present invention.

[0041] In the figure, 1. Sampling container; 2. Air pump; 3. First pipeline; 4. Second pipeline; 5. Centrifuge container; 501. Centrifuge container inlet; 502. Centrifuge container outlet; 503. Sampling port; 6. First one-way valve; 7. Second one-way valve; 801. Signal induction source; 802. Signal inductor; 9. Sterile filter; 10. Third pipeline. Specific embodiments

[0042] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present invention.

[0043] In one embodiment, a sampling device is provided, which includes a sampling container 1, an air pump 2, a first pipeline 3, and a second pipeline 4. The first pipeline 3 is used to connect a centrifugal container 5 and the sampling container 1, and a first one-way valve 6 is provided on the first pipeline 3. The second pipeline 4 is used to connect the sampling container 1 and a cell counting device, and a second one-way valve 7 is provided on the second pipeline 4. The air pump 2 is connected to the sampling container 1. When the air pump 2 is in the air extraction state, the first one-way valve 6 is opened and the second one-way valve 7 is closed, so that the liquid to be sampled flows from the centrifugal container 5 into the sampling container 1 through the first pipeline 3. When the air pump 2 is in the air blowing state, the first one-way valve 6 is closed and the second one-way valve 7 is opened, so that the liquid to be sampled flows from the sampling container 1 into the cell counting device through the second pipeline 4.

[0044] Among them, the liquid to be sampled is a cell sample liquid that needs to be sampled and is stored in the centrifugal container 5.

[0045] As an example, as Figure 1 shown, it is a connection relationship diagram of the sampling device and the centrifugal container 5. One end of the first pipeline 3 is connected to the sampling port 503 of the centrifugal container 5, and the other end of the first pipeline 3 is connected to the first end of the sampling container 1, which is used to make the liquid to be sampled flow between the centrifugal container 5 and the sampling container 1. A first one-way valve 6 is provided on the first pipeline 3, which is used to control the liquid to be sampled to flow only unidirectionally from the centrifugal container 5 to the sampling container 1 and cannot flow reversely, facilitating the sampling of the liquid to be sampled and preventing reverse contamination.

[0046] One end of the second pipeline 4 is connected to the second end of the sampling container 1, and the other end of the second pipeline 4 is connected to a cell counting device outside the sampling device, which is used to make the liquid to be sampled flow between the sampling container 1 and the cell counting device. A second one-way valve 7 is provided on the second pipeline 4, which is used to control the liquid to be sampled to flow only unidirectionally from the sampling container 1 through the second one-way valve 7 to the external cell counting device, preventing reverse contamination, and facilitating the counting and other processing of the liquid to be sampled by using an external cell counting device after the sampling is completed.

[0047] The air pump 2 is connected to the sampling container 1, which is convenient for evacuating the entire sampling device when the air pump 2 is in the air extraction state, realizing the control of the liquid to be sampled to flow from the centrifugal container 5 into the sampling container 1 through the first pipeline 3, or blowing the entire sampling device when the air pump 2 is in the air blowing state, and outputting the liquid to be sampled from the second end of the sampling container 1 to an external cell counting device through the second pipeline 4.

[0048] Specifically, when the air pump 2 is in the air extraction state, the gas in the sampling device is extracted from the sampling device. Since the first one-way valve 6 is in a non-open state when the pressure is relatively small, the air extraction will reduce the internal pressure between the first one-way valve 6 and the air pump 2. Since the flow direction of the first one-way valve 6 is from the centrifuge container 5 to the sampling container 1, a pressure difference in the same direction as the flow direction of the first one-way valve 6 is formed on both sides of the valve between the outlet and the inlet of the first one-way valve 6. When the pressure difference reaches the pressure value required to open the first one-way valve 6, the first one-way valve 6 in the sampling device opens. And since the flow direction of the second one-way valve 7 is from the sampling container 1 to the external cell counting device, the pressure difference formed on both sides of the second one-way valve 7 is opposite to the flow direction of the second one-way valve 7. Therefore, when the air pump 2 is in the air extraction state, the second one-way valve 7 is in the closed state. Therefore, when the air pump 2 is in the air extraction state, the liquid to be sampled flows from the centrifuge container 5 into the sampling container 1 through the first pipeline 3, and will not flow out of the sampling container.

[0049] When the air pump 2 is in the air blowing state, external gas is blown into the sampling device. Since the second one-way valve 7 is in a non-open state when the pressure is relatively small, the air blowing will increase the internal pressure between the second one-way valve 7 and the air pump 2. Since the flow direction of the second one-way valve 7 is from the sampling container 1 to the external cell counting device, a pressure difference in the same direction as the flow direction of the second one-way valve 7 is formed on both sides of the valve between the outlet and the inlet of the second one-way valve 7. When the pressure difference reaches the pressure value required to open the second one-way valve 7, the second one-way valve 7 in the sampling device opens. And since the flow direction of the first one-way valve 6 is from the centrifuge container 5 to the sampling container 1, when the air pump 2 blows air, the pressure difference formed on both sides of the first one-way valve 6 is opposite to the flow direction of the first one-way valve 6. Therefore, when the air pump 2 is in the air blowing state, the first one-way valve 6 is in the closed state. Therefore, when the air pump 2 is in the air blowing state, the liquid to be sampled flows from the sampling container 1 into the cell counting device through the second pipeline 4, and the liquid to be sampled in the centrifuge container 5 will not flow towards the sampling container 1, which is convenient for counting the cells in the liquid to be sampled by the cell counting device.

[0050] In this embodiment, by controlling the air blowing state and the air extraction state of the air pump 2 in the sampling device, the sampling of the liquid to be sampled can be made more accurate. Moreover, this sampling device can achieve the sampling purpose without manual intervention and without opening, with simple and convenient operation, and can achieve aseptic sampling.

[0051] In one embodiment, the sampling device further includes a monitoring and control module, which is connected to the air pump 2 and is used to control the air pump 2 to switch between the air extraction state and the air blowing state according to the current monitoring data. Further, the monitoring and control module includes a sensing unit and a control unit. The sensing unit is arranged on the sampling container 1 and is used to collect the current monitoring data of the liquid to be sampled in the sampling container 1. The control unit is connected to the sensing unit and the air pump 2 and is used to control the air pump 2 to switch between the air extraction state and the air blowing state according to the current monitoring data.

[0052] Wherein, the current monitoring data refers to the monitoring data generated by monitoring the sampling device through the sensing unit and is used to control the air pump 2 to switch between the air extraction state and the air blowing state.

[0053] As an example, the sampling device further includes a monitoring and control module, which is used to monitor the current monitoring data in the sampling device in real time during the sampling process of the liquid to be sampled in the centrifuge container 5. The monitoring and control module is connected to the air pump 2, which is convenient for controlling the air pump 2 to switch the working state according to the current monitoring data. When the current monitoring data meets the preset switching condition, the air pump 2 is controlled to switch from the air extraction state to the air blowing state. When the current monitoring data does not meet the preset switching condition, the air pump 2 is controlled to be in the air extraction state. Wherein, the preset switching condition is a preset condition for controlling the air pump 2 to perform state conversion.

[0054] Further, the monitoring and control module includes a sensing unit and a control unit. The sensing unit is arranged on the sampling container 1 and is used to collect the current monitoring data corresponding to the liquid to be sampled in the sampling container 1. The sensing unit is connected to the control unit and is used to transmit the collected current monitoring data to the control unit. The control unit is connected to the air pump 2. When the current monitoring data meets the preset switching condition, the air pump 2 is controlled to switch from the air extraction state to the air blowing state. When the current monitoring data does not meet the preset switching condition, the air pump 2 is controlled to be in the air extraction state.

[0055] In this embodiment, by setting a monitoring and control module including a sensing unit and a control unit in the sampling device, the current monitoring data is obtained through the sensing unit, and the air pump 2 is enabled by the control unit to switch between the air extraction state and the air blowing state according to the current monitoring data, without manual operation, which is relatively convenient and efficient, and can also control the sampling device to perform relatively accurate sampling.

[0056] In one embodiment, the sensing unit includes a signal induction source 801 and a signal sensor 802. The signal induction source 801 and the signal sensor 802 are respectively arranged on both sides of the sampling container 1 and are used to collect the current liquid volume of the liquid to be sampled in the sampling container 1. The control unit is connected to the signal sensor 802 and is used to control the air pump 2 to be in the air blowing state when the current liquid volume is greater than the preset liquid volume; when the current liquid volume is not greater than the preset liquid volume, the air pump 2 is controlled to be in the air extraction state.

[0057] Wherein, the current liquid volume refers to the volume of the liquid to be sampled in the sampling container 1 currently monitored when the air pump 2 is in the air extraction state. The preset liquid volume refers to the volume of the liquid to be sampled that is predetermined and required for sampling, and is used to control the air pump 2 to switch states.

[0058] As an example, the signal induction source 801 and the signal inductor 802 are respectively arranged on both sides of the sampling container 1. The signal induction source 801 generates an optical signal on the sampling container 1, and the signal inductor 802 determines the current liquid volume of the liquid to be sampled in the sampling container 1 by collecting the optical signal; the control unit is connected to the signal inductor 802 and is used to control the air pump 2 to be in the air blowing state when the current liquid volume is greater than the preset liquid volume; when the current liquid volume is not greater than the preset liquid volume, control the air pump 2 to be in the air extraction state.

[0059] In this embodiment, the signal induction source 801 and the signal inductor 802 are respectively arranged on both sides of the sampling container 1 for collecting the current liquid volume of the liquid to be sampled in the sampling container 1. The control unit is connected to the signal inductor 802, which is convenient for controlling the sampling device to perform more accurate sampling according to whether the current liquid volume of the liquid to be sampled collected is greater than the preset liquid volume.

[0060] In another embodiment, the induction unit includes a pressure sensor arranged in the sampling container 1 for collecting the current liquid pressure of the liquid to be sampled in the sampling container 1; the control unit is connected to the pressure sensor and is used to control the air pump 2 to be in the air blowing state when the current liquid pressure is greater than the preset liquid pressure; when the current liquid pressure is not greater than the preset liquid pressure, control the air pump 2 to be in the air extraction state.

[0061] Wherein, the current liquid pressure refers to the liquid pressure of the liquid to be sampled in the sampling container 1 currently monitored when the air pump 2 is in the air extraction state. The preset liquid pressure refers to the liquid pressure of the liquid to be sampled that is predetermined and required for sampling, and is used to control the air pump 2 to switch states.

[0062] As an example, a pressure sensor is arranged in the sampling container 1 for collecting the current liquid pressure of the liquid to be sampled in the sampling container 1. The control unit is connected to the pressure sensor. When it is determined that the current liquid pressure is greater than the preset liquid pressure, control the air pump 2 to be in the air blowing state; when the current liquid pressure is not greater than the preset liquid pressure, control the air pump 2 to be in the air extraction state to control the sampling device to perform more accurate sampling.

[0063] In this embodiment, the pressure sensor is disposed inside the sampling container 1 to collect the current liquid pressure of the liquid to be sampled in the sampling container 1. The control unit controls the working state of the air pump 2 according to the current liquid pressure, without manual participation, and can achieve relatively accurate aseptic sampling of the liquid to be sampled.

[0064] In this embodiment, the signal induction source 801 and the signal inductor 802 are oppositely disposed on both sides of the sampling container 1 for determining the current monitoring data of the liquid to be sampled in the sampling container 1, facilitating relatively accurate sampling by controlling the sampling device according to the obtained current monitoring data of the liquid to be sampled.

[0065] In one embodiment, the first one-way valve 6 and the second one-way valve 7 are aseptic one-way valves.

[0066] As an example, as Figure 1 shown, both the first one-way valve 6 disposed on the first pipeline 3 and the second one-way valve 7 disposed on the second pipeline 4 are aseptic one-way valves. In this example, as aseptic one-way valves, the first one-way valve 6 and the second one-way valve 7 can ensure that the liquid to be sampled is not contaminated by miscellaneous bacteria each time the liquid to be sampled is sampled, achieving the purpose of aseptic sampling of the liquid to be sampled.

[0067] In one embodiment, the sampling device further includes a third pipeline 10 for connecting the sampling container 1 and the air pump 2, and an aseptic filter 9 is provided on the third pipeline 10.

[0068] As an example, as Figure 1 shown, in the sampling device, the third end of the sampling container 1 is connected to the air pump 2 through the third pipeline 10, used to draw out the gas from the sampling device through the third pipeline 10 when the air pump 2 is in the air extraction state, so that the first one-way valve 6 on the first pipeline 3 is opened, and the liquid to be sampled enters the sampling container 1 from the centrifuge container 5 through the first pipeline 3; when the air pump 2 is in the air blowing state, the gas is blown from the third pipeline 10 into the second pipeline 4 through the sampling container 1, so that the second one-way valve 7 is opened, and the liquid to be sampled is output from the sampling container 1 to an external cell counting device through the second pipeline 4. The aseptic filter 9 is provided on the third pipeline 10, used to ensure that the gas blown into the sampling device is aseptic gas when the air pump 2 is in the air blowing state, ensuring that the sampling device is in an aseptic environment.

[0069] In this embodiment, the sampling container 1 and the air pump 2 are connected through the third pipeline 10, which can achieve the functions of the corresponding working states when the air pump 2 is in different working states, without manual participation in sampling, and can realize the process of automatic sampling; the aseptic filter 9 is provided on the third pipeline 10, which can ensure that the sampling device is in an aseptic environment, facilitating aseptic sampling.

[0070] The embodiment of the present invention provides a sampling method, which can be applied inFigure 1 In the sampling device shown, it is used to achieve the purpose of relatively precise aseptic sampling.

[0071] In one embodiment, as Figure 2 shown, a sampling method is provided, which is controlled by the sampling controller in Figure 5 and is illustrated by taking the sampling implemented by the sampling device in Figure 1 as an example, and includes the following steps:

[0072] S201: In response to the received sampling instruction, control the air pump 2 to be in the air extraction state, so that the first one-way valve 6 opens and the second one-way valve 7 closes, so that the liquid to be sampled flows from the centrifuge container 5 into the sampling container 1 through the first pipeline 3;

[0073] S202: Obtain the current monitoring data. When the current monitoring data meets the preset switching condition, control the air pump 2 to be in the air blowing state, so that the first one-way valve 6 closes and the second one-way valve 7 opens, so that the liquid to be sampled flows from the sampling container 1 into the cell counting device through the second pipeline 4.

[0074] Among them, the sampling instruction is used to control whether sampling can start. Understandably, this sampling method is controlled by the sampling controller to achieve sampling, and this process does not require manual participation in the specific sampling process. Therefore, after the sampling controller receives the sampling instruction, it starts to control the sampling device to execute the sampling method in this embodiment to achieve sampling of the liquid to be sampled.

[0075] As an example, in step S201, when the sampling controller receives the sampling instruction, it responds to the sampling instruction, controls the air pump 2 to be in the air extraction state, extracts the gas in the sampling device, and the second one-way valve 7 is in the closed state. When the pressure received by the first one-way valve 6 in the first pipeline 3 reaches the pressure required to open the first one-way valve 6, the first one-way valve 6 opens, so that the liquid to be sampled flows from the sampling port 503 of the centrifuge container 5 into the sampling container 1 through the first pipeline 3 and will not flow out of the sampling container 1. Understandably, due to the setting of the connection method of the sampling device, since the inlet of the second one-way valve 7 is on the second pipeline 4 close to the direction where the sampling container 1 is located, when the air pump 2 is in the air extraction state, no matter how large the pressure in the sampling device reaches, the second one-way valve 7 is in the closed state. This connection method of the sampling device can ensure that the liquid to be sampled will not flow out of the sampling container 1 during the process of extracting the liquid to be sampled from the centrifuge container 5 to the sampling container 1, which is convenient for obtaining relatively precise current monitoring data later and can make the sampled liquid to be sampled more precise.

[0076] As an example, in step S202, during the process that the sampling controller samples from the centrifugal container 5 with the air pump 2 in the air extraction state, the current monitoring data is obtained in real time to determine whether the current monitoring data meets the preset switching condition. When it is determined that the current monitoring data meets the preset switching condition, the air pump 2 is controlled to switch from the air extraction state to the air blowing state, the first one-way valve 6 is closed, and when the pressure received by the second one-way valve 7 in the second pipeline 4 reaches the pressure required to open the second one-way valve 7, the second one-way valve 7 is opened, so that the sampling liquid flows from the sampling container 1 into the cell counting device through the second pipeline 4. For example, the current monitoring data can be the pressure value of the liquid to be sampled in the sampling container 1, and the preset switching condition is used to determine whether the pressure value of the liquid to be sampled in the sampling container 1 meets the preset pressure condition. When it is determined that the pressure value of the liquid to be sampled in the sampling container 1 meets the preset pressure condition, the air pump 2 is controlled to switch from the air extraction state to the air blowing state, the first one-way valve 6 is closed, and when the pressure received by the second one-way valve 7 in the second pipeline 4 reaches the pressure required to open the second one-way valve 7, the second one-way valve 7 is opened, so that the sampling liquid flows from the sampling container 1 into the cell counting device through the second pipeline 4, realizing relatively accurate aseptic sampling.

[0077] In this example, there is no need for manual participation in sampling, and there is no need to frequently open the sampling device. Only by switching the air pump 2 between the air extraction state and the air blowing state can the automatic sampling of the liquid to be sampled be realized and the sampling be output, which can ensure the aseptic environment of the sampling device and ensure aseptic sampling. Moreover, by obtaining the current monitoring data to control the switching of the air pump 2 between the air extraction state and the air blowing state, relatively accurate sampling is realized.

[0078] In this embodiment, according to the current monitoring data in real time during the sampling process, the air pump 2 is switched between the air extraction state and the air blowing state, without the need for manual participation in the specific sampling process and without frequently opening the sampling device, which can ensure that the sampling device is in an aseptic environment and realize relatively accurate aseptic sampling.

[0079] In one embodiment, the current monitoring data is the current air extraction duration.

[0080] Wherein, the current air extraction duration refers to the duration when the air pump 2 is in the air extraction state. Understandably, due to the certainty of the specification of the first pipeline 3, the duration when the air pump 2 is in the air extraction state can reflect the volume of the liquid to be sampled in the sampling container 1. Therefore, it can be determined whether to control the air pump 2 to switch from the air extraction state to the air blowing state by determining whether the current air extraction duration reaches the preset duration. The preset duration is used to judge whether the state of the air pump 2 can be switched.

[0081] In one embodiment, as Figure 3 shown, in step S202, that is, when the current monitoring data meets the preset switching condition, controlling the air pump 2 to be in the air blowing state includes:

[0082] S301: If the current air extraction duration reaches the preset duration, control the air pump 2 to be in the air blowing state;

[0083] S302: If the current air extraction duration does not reach the preset duration, control the air pump 2 to be in the air extraction state.

[0084] As an example, in step S301, the sampling controller monitors the current air extraction duration of the air pump 2 in real time. When it is determined that the current air extraction duration reaches the preset duration, control the air pump 2 to switch from the air extraction state to the air blowing state. Understandably, since the specification of the first pipeline 3 is known, the volume of the liquid to be sampled extracted in the preset duration can reach the required sampling volume. Therefore, when it is determined that the current air extraction duration reaches the preset duration, control the air pump 2 to switch from the air extraction state to the air blowing state, which is convenient for controlling the liquid to be sampled to flow from the sampling container 1 through the second pipeline 4 into the cell counting device to complete aseptic sampling.

[0085] As an example, in step S302, the sampling controller monitors the current air extraction duration of the air pump 2 in real time. When it is determined that the current air extraction duration does not reach the preset duration, control the air pump 2 to remain in the air extraction state. Understandably, since the specification of the first pipeline 3 is known, the volume of the liquid to be sampled extracted in the preset duration can reach the required sampling volume. Therefore, when it is determined that the current air extraction duration does not reach the preset duration, it indicates that the volume of the liquid to be sampled in the sampling container 1 does not reach the required sampling volume, and control the air pump 2 to remain in the air extraction state until it is determined that the current air extraction duration reaches the preset duration. In this example, when it is determined that the current air extraction duration does not reach the preset duration, control the air pump 2 to remain in the air extraction state, which is convenient for obtaining a more accurate liquid to be sampled and realizing accurate sampling of the liquid to be sampled.

[0086] In this embodiment, according to whether the current air extraction duration reaches the preset duration, control the state of the air pump 2, which is convenient for obtaining a more accurate liquid to be sampled and realizing accurate sampling of the liquid to be sampled.

[0087] In another embodiment, the current monitored data is the current liquid volume of the liquid to be sampled in the sampling container 1.

[0088] Understandably, the current liquid volume in the sampling container 1 is monitored in real time to control the air pump 2 to switch states and realize more accurate sampling of the liquid to be sampled.

[0089] In another embodiment, as Figure 4 shown, in step S202, that is, when the current monitored data meets the preset switching condition, control the air pump 2 to be in the air blowing state, including:

[0090] S401: If the current liquid volume reaches the preset liquid volume, control the air pump 2 to be in the air blowing state;

[0091] S402: If the current liquid volume has not reached the preset liquid volume, control the air pump 2 to be in the air extraction state.

[0092] As an example, in step S401, the sampling controller monitors the current liquid volume in the sampling container 1 in real time. When it is determined that the current liquid volume has reached the preset liquid volume, control the air pump 2 to switch from the air extraction state to the air blowing state. In this example, when it is determined that the current liquid volume has reached the preset liquid volume, controlling the air pump 2 to switch from the air extraction state to the air blowing state facilitates controlling the liquid to be sampled to flow from the sampling container 1 into the cell counting device through the second pipeline 4, completing a relatively accurate aseptic sampling.

[0093] As an example, in step S402, the sampling controller monitors the current liquid volume in the sampling container 1 in real time. When it is determined that the current liquid volume has not reached the preset liquid volume, control the air pump 2 to remain in the air extraction state until it is determined that the current liquid volume has reached the preset liquid volume. In this example, when it is determined that the current liquid volume has not reached the preset liquid volume, controlling the air pump 2 to remain in the air extraction state facilitates obtaining a relatively accurate liquid to be sampled and realizing accurate sampling of the liquid to be sampled.

[0094] In this embodiment, according to whether the current liquid volume has reached the preset liquid volume, control the state of the air pump 2, which facilitates obtaining a relatively accurate liquid to be sampled and realizing accurate sampling of the liquid to be sampled.

[0095] It should be understood that the magnitudes of the sequence numbers of the steps in the above embodiments do not mean the order of execution. The order of execution of each process should be determined according to its function and internal logic, and should not constitute any limitation to the implementation process of the embodiments of the present invention.

[0096] In one embodiment, a sampling controller is provided. The sampling controller may be a server, and its internal structure diagram may be as Figure 5 shown. The sampling controller includes a processor, a memory, a network interface, and a database connected through a system bus. Among them, the processor of the sampling controller is used to provide computing and control capabilities. The memory of the sampling controller includes a non-volatile storage medium and an internal memory. The non-volatile storage medium stores an operating system, a computer program, and a database. The internal memory provides an environment for the operation of the operating system and the computer program in the non-volatile storage medium. The database of the sampling controller is used to store the data used or generated during the execution of the sampling method. The network interface of the sampling controller is used to communicate with an external terminal through a network connection. When the computer program is executed by the processor, a sampling method is implemented.

[0097] In one embodiment, a sampling controller is provided, which includes a memory, a processor, and a computer program stored on the memory and executable on the processor. When the processor executes the computer program, the sampling method in the above embodiment is implemented. For example Figure 2 as shown in S201 - S202, or Figure 3 and Figure 4 as shown in, to avoid repetition, it will not be elaborated here.

[0098] In one embodiment, a sampling control system is provided, which includes the above sampling device, the above sampling controller, and a centrifuge container 5. The sampling controller is respectively connected to the sampling device and the centrifuge container 5 for implementing the above sampling method.

[0099] As an example, the sampling control system includes a sampling device, a sampling controller, and a centrifuge container 5. The sampling controller is respectively connected to the sampling device and the centrifuge container 5. When responding to a received sampling instruction, the sampling controller controls the air pump 2 of the sampling device to be in the air extraction state, so that the first one - way valve 6 opens and the second one - way valve 7 closes, enabling the liquid to be sampled to flow from the centrifuge container 5 into the sampling container 1 through the first pipeline 3; obtains the current monitoring data, and when the current monitoring data meets the preset switching condition, controls the air pump 2 to be in the air blowing state, so that the first one - way valve 6 closes and the second one - way valve 7 opens, enabling the liquid to be sampled to flow from the sampling container 1 into the cell counting device through the second pipeline 4, realizing the sampling of the liquid to be sampled in the centrifuge container 5.

[0100] In this embodiment, the sampling controller is respectively connected to the sampling device and the centrifuge container 5 to control the sampling device and the centrifuge container 5 to achieve relatively precise aseptic sampling.

[0101] In one embodiment, a cell processing device is provided, which includes the above sampling control system and a cell counting device; an air - permeable hole is provided on the centrifuge container 5, and an air - permeable membrane is provided on the air - permeable hole for enabling the centrifuge container 5 to obtain sterile air; the sampling controller is connected to the centrifuge container 5 for controlling the centrifuge container inlet 501 and the centrifuge container outlet 502 to close after receiving a sampling instruction, and controlling the centrifuge container 5 to continue to be in the working state; the cell counting device is used for counting the cells in the liquid to be sampled flowing into the cell counting device.

[0102] As an example, the cell processing device includes a sampling control system and a cell counting device.

[0103] Among them, an air - permeable hole is provided on the centrifuge container 5 of the sampling control system, and an air - permeable membrane is provided on the air - permeable hole. Specifically, a plurality of air - permeable holes can be provided on the rotation axis of the bottom of the centrifuge container 5, and an air - permeable membrane is provided on each air - permeable hole, or a plurality of air - permeable holes can be provided on the top of the centrifuge container 5, and an air - permeable membrane is provided on each air - permeable hole, so that the centrifuge container 5 can obtain sterile air and ensure the cell activity in the centrifuge container 5.

[0104] Among them, the sampling controller is connected to the centrifuge container 5. After receiving the sampling instruction, the sampling controller controls the centrifuge container inlet 501 and the centrifuge container outlet 502 of the centrifuge container 5 to close, and controls the centrifuge container 5 to continue to be in the working state. Understandably, after receiving the sampling instruction, the sampling controller controls the centrifuge container inlet 501 and the centrifuge container outlet 502 of the centrifuge container 5 to close, preventing air with contaminants from entering the centrifuge container 5 during the sampling process, ensuring sampling in a sterile environment, and controlling the centrifuge container 5 to continue to be in the working state, which can ensure that the cells in the liquid to be sampled in the centrifuge container 5 maintain cell viability and reduce the detection error when counting and detecting the cells in the liquid to be sampled after sampling. In this example, since the centrifuge container 5 continues to work during the sampling process, the liquid to be sampled flows along the upper side of the inner wall of the centrifuge container 5, and the sampling pipeline between the sampling port 503 and the centrifuge container 5 can be placed on the upper side of the inner wall of the centrifuge container 5 for sampling, without the need to enter the lower side or the bottom inside the centrifuge container 5 for sampling, which can not only save the sampling pipeline, but also sample evenly.

[0105] Among them, the second pipeline 4 of the sampling device is used to connect the sampling container 1 and the cell counting device, and the cell counting device is used to count the cells in the liquid to be sampled flowing into the cell counting device. Specifically, when the air pump 2 of the sampling device is in the blowing state, the liquid to be sampled in the sampling container 1 flows into the container of the area to be detected or the EP (eppendorf) test tube of the cell counting device through the second pipeline 4. If the liquid to be sampled flows into the container of the area to be detected, the cells in the liquid to be sampled in the container of the area to be detected are directly counted, and then indicators such as cell viability, loss rate, and density are judged. If the liquid to be sampled flows into the EP test tube, the EP test tube needs to be heat-sealed and then the cells in the liquid to be sampled in the heat-sealed EP test tube are counted, and then indicators such as cell viability, loss rate, and density are judged.

[0106] The cell processing device provided in this embodiment facilitates the realization of cell counting of the liquid to be sampled and the judgment of cell indicators.

[0107] The above-described embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the various embodiments of the present invention, and should all be included in the protection scope of the present invention.

Claims

1. A sampling device, characterized in that, It includes a sampling container, an air pump, a first pipeline, and a second pipeline; The first pipeline is used to connect the centrifuge container and the sampling container, and a first one-way valve is provided on the first pipeline; The second pipeline is used to connect the sampling container and the cell counting device, and a second one-way valve is provided on the second pipeline; The air pump is connected to the sampling container. When the air pump is in the air extraction state, the first one-way valve is opened and the second one-way valve is closed, so that the liquid to be sampled flows from the centrifuge container into the sampling container through the first pipeline; When the air pump is in the air blowing state, the first one-way valve is closed and the second one-way valve is opened, so that the liquid to be sampled flows from the sampling container into the cell counting device through the second pipeline.

2. The sampling device according to claim 1, characterized in that, The sampling device further includes a monitoring and control module, which is connected to the air pump and is used to control the air pump to switch between the air extraction state and the air blowing state according to the current monitoring data; Further, the monitoring and control module includes a sensing unit and a control unit; The sensing unit is arranged on the sampling container and is used to collect the current monitoring data of the liquid to be sampled in the sampling container; The control unit, which is connected to the sensing unit and the air pump, is used to control the air pump to switch between the air extraction state and the air blowing state according to the current monitoring data.

3. The sampling device according to claim 2, characterized in that, The sensing unit includes a signal induction source and a signal sensor, and the signal induction source and the signal sensor are respectively arranged on both sides of the sampling container and are used to collect the current liquid volume of the liquid to be sampled in the sampling container; The control unit, which is connected to the signal sensor, is used to control the air pump to be in the air blowing state when the current liquid volume is greater than the preset liquid volume; When the current liquid volume is not greater than the preset liquid volume, the air pump is controlled to be in the air extraction state.

4. The sampling device according to claim 2, characterized in that, The sensing unit includes a pressure sensor arranged in the sampling container and is used to collect the current liquid pressure of the liquid to be sampled in the sampling container; The control unit, which is connected to the pressure sensor, is used to control the air pump to be in the air blowing state when the current liquid pressure is greater than the preset liquid pressure; When the current liquid pressure is not greater than the preset liquid pressure, the air pump is controlled to be in the air extraction state.

5. A sampling method, characterized in that, Applied to the sampling device according to any one of claims 1-4, the sampling method includes: In response to the received sampling instruction, control the air pump to be in the air extraction state, so that the first one-way valve is opened and the second one-way valve is closed, so that the liquid to be sampled flows from the centrifuge container into the sampling container through the first pipeline; Obtain the current monitoring data. When the current monitoring data meets the preset switching condition, control the air pump to be in the air blowing state, so that the first one-way valve is closed and the second one-way valve is opened, so that the liquid to be sampled flows from the sampling container into the cell counting device through the second pipeline.

6. The sampling method according to claim 5, wherein The current monitoring data is the current air extraction duration; The controlling the air pump to be in the air blowing state when the current monitoring data meets the preset switching condition includes: If the current air extraction duration reaches the preset duration, then control the air pump to be in the air blowing state; If the current air extraction duration has not reached the preset duration, control the air pump to be in the air extraction state.

7. The sampling method according to claim 5, wherein The current monitoring data is the current liquid volume of the liquid to be sampled in the sampling container; When the current monitoring data meets the preset switching condition, controlling the air pump to be in the air blowing state includes: If the current liquid volume reaches the preset liquid volume, control the air pump to be in the air blowing state; If the current liquid volume has not reached the preset liquid volume, control the air pump to be in the air extraction state.

8. A sampling controller, comprising a memory, a processor, and a computer program stored in the memory and executable on the processor, characterized in that, When the processor executes the computer program, it implements the sampling method according to any one of claims 5 to 7.

9. A sampling control system, characterized in that, It includes the sampling device according to any one of claims 1 to 4, the sampling controller according to claim 8, and a centrifuge container. The sampling controller is respectively connected to the sampling device and the centrifuge container, and is used to implement the sampling method according to any one of claims 5 to 7.

10. A cell processing device, characterized in that, It includes the sampling control system according to claim 9 and a cell counting device; A ventilation hole is provided on the centrifuge container, and a ventilation membrane is provided on the ventilation hole for the centrifuge container to obtain sterile air; The sampling controller is connected to the centrifuge container and is used to control the centrifuge container inlet and the centrifuge container outlet to close after receiving a sampling instruction, and control the centrifuge container to continue to be in the working state; The cell counting device is used to count the cells in the liquid to be sampled flowing into the cell counting device.

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