Stem cell exosome extraction and storage device

Abstract

The invention relates to the technical field of exosome extraction and storage, in particular to a stem cell exosome extraction and storage device which comprises a centrifugal machine body used for conducting ultracentrifugation on stem cell exosomes, a centrifugal cavity is formed in the centrifugal machine body, and a rotating disc is arranged in the centrifugal cavity in a rotating fit mode; a placing rack for placing test tubes is arranged on the rotating disc, the test tubes are used for accommodating an exosome extracting solution, and a clamping mechanism for placing a plurality of test tubes is arranged on the placing rack; a cold air conveying mechanism for refrigeration is arranged on the inner wall of the centrifugal cavity, and a sealing mechanism for isolating single test tubes is arranged at the top of the centrifugal cavity; the method is used for reducing interference on the surrounding environment temperature in the exosome taking-out process, improving the temperature stability after exosome separation and guaranteeing the stability of the exosome structure in the centrifugal-to-storage process.

Description

Technical Field

[0001] The present invention relates to the technical field of exosome extraction and storage, and particularly relates to a device for extracting and storing stem cell exosomes. Background Art

[0002] In the methods for extracting stem cell exosomes, it mainly includes ultracentrifugation, density gradient centrifugation, precipitation method, etc. Among them, ultracentrifugation is currently the "gold standard" for exosome separation. Its advantages are that the method is mature, applicable to large-volume samples, the obtained exosomes have high purity and large yield.

[0003] However, the centrifugal force generated during ultracentrifugation may cause a certain degree of damage to the exosome structure, although this damage may not be significant in most cases. However, during storage, if the conditions are improper (such as too high temperature, too long time, etc.), the bilayer lipid membrane structure of exosomes may be further damaged, thus affecting its integrity and function. Therefore, during the storage of exosomes, it is usually considered to add protective agents to improve the stability of exosomes, such as glycerol, DMSO, etc.; use buffers such as PBS or HBSS to maintain an appropriate pH value and ionic strength to further improve the stability of exosomes.

[0004] During the centrifugation-to-freezing preservation process, it is usually necessary to first centrifuge the exosomes at 4°C to remove cell debris, then transfer them to pre-cool at -20°C for a period of time, and finally store them in a -80°C refrigerator for a long time, which can effectively reduce the damage to exosomes during the freezing process. The heat effect generated during centrifugation and the non-uniformity of heat transfer will cause a temperature difference between the center and the edge, resulting in increased damage to the exosome structure due to thermal stimulation during the storage of exosomes. Therefore, the present invention provides a device for extracting and storing stem cell exosomes to improve the temperature stability after exosome separation and ensure the stability of the exosome structure. Summary of the Invention

[0005] To solve the above problems, the present invention provides a device for extracting and storing stem cell exosomes, which is used to reduce the interference to the surrounding environment during the exosome extraction process, improve the temperature stability after exosome separation, and ensure the stability of the exosome structure during the centrifugation-to-storage process.

[0006] To achieve the above object, the technical solution of the present invention is as follows: A device for extracting and storing stem cell exosomes, including a centrifuge body for ultracentrifuging stem cell exosomes. There is a centrifugation chamber opened in the centrifuge body, and a turntable is rotatably fitted in the centrifugation chamber; a placement rack for placing test tubes is provided on the turntable. The test tubes are used to accommodate exosome extraction liquid, and a clamping mechanism for placing a plurality of test tubes is provided on the placement rack;

[0007] An air-cooling delivery mechanism for refrigeration is provided on the inner wall of the centrifugal chamber, and a sealing mechanism for isolating individual test tubes is provided at the top of the centrifugal chamber.

[0008] Furthermore, the clamping mechanism includes a clamping shaft fixedly connected to the center of the bottom of the placement rack. A sliding groove is formed at one end of the clamping shaft away from the placement rack.

[0009] A central shaft is clamped in the sliding groove and is fixedly connected to the center of the turntable. A number of receiving grooves are also fixedly connected to the bottom of the placement rack and are arranged in a ring with the clamping shaft as the center. The test tube is located in the receiving groove.

[0010] Furthermore, a receiving cavity is formed inside the receiving groove. The diameter of the receiving cavity at one end close to the placement rack is larger than the diameter of the receiving cavity at one end away from the placement rack. A number of load-bearing plates with different diameters are slidably fitted in the receiving cavity. A rubber ring is fixedly connected to the center of the load-bearing plate, and the rubber ring abuts against the test tube.

[0011] Furthermore, the air-cooling delivery mechanism includes an exchange port communicated with the centrifugal chamber. The exchange ports are arranged in a ring with the centrifugal chamber as the center. The exchange port is communicated with a refrigerator for cooling through a trachea.

[0012] The refrigerator is electrically connected to a control panel located inside the centrifuge. A temperature sensor for measuring the internal temperature of the centrifugal chamber in real time is fixedly connected to the top of the centrifugal chamber. The temperature sensor is electrically connected to the control panel, and the control panel is used to control the operation of the refrigerator based on the internal temperature of the centrifugal chamber.

[0013] Furthermore, the sealing mechanism includes a cover plate detachably connected to the centrifuge body. A rotating plate is slidably fitted at the center of the cover plate. The rotating plate is located above the placement rack, and a shielding plate is rotatably fitted on the rotating plate.

[0014] One side of the shielding plate away from the center of the rotating plate is rotatably fitted with the rotating plate. An isolation plate is fixedly connected to the bottom of the rotating plate and is arranged circumferentially with the shielding plate as the center.

[0015] Furthermore, a compression chamber is provided between the central shaft and the clamping shaft. The compression chamber is communicated with the sliding groove. The compression chamber is communicated with a pressure relief pipe. A one-way valve and a solenoid valve are connected between the pressure relief pipe and the compression chamber. The one-way valve can only allow the air in the pressure relief pipe to flow into the compression chamber. The solenoid valve is electrically connected to the control panel and is normally closed under normal conditions.

[0016] A transfer pipe communicated with the pressure relief pipe is formed on the isolation plate. The transfer pipe and the pressure relief pipe are located at the center of the centrifugal chamber. One end of the transfer pipe away from the pressure relief pipe is detachably connected with a plug, and the plug is located on the side of the rotating plate away from the isolation plate.

[0017] When the placement rack reaches the topmost point, the placement rack abuts against the isolation plate. When the placement rack moves to the lowest point, the placement rack separates from the isolation plate.

[0018] Further, a transparent glass layer is provided on the rotating plate.

[0019] Further, the temperature sensor is located on the isolation plate;

[0020] The temperature sensor is used to measure the real-time temperature value inside the isolation plate in real time and send the real-time temperature value to the control panel; the control panel is used to compare the real-time temperature value with the set standard value. If the real-time temperature is greater than the standard value, a maintenance instruction is sent to the refrigerator; if the real-time temperature is less than or equal to the standard value, a stop instruction is sent to the refrigerator, and a centrifugation instruction is sent to the centrifuge body;

[0021] The control panel is further used to compare the real-time temperature value with the set warning value after the centrifuge body completes the centrifugation instruction. If the real-time temperature value is greater than the warning value, a start instruction is sent to the refrigerator, and an alarm instruction is sent to the centrifuge body; if the real-time temperature value is less than or equal to the warning value, a maintenance instruction is sent to the refrigerator.

[0022] Further, a gasket is provided between the clamping shaft and the central shaft.

[0023] Further, one end of the receiving groove away from the placement rack is higher than the end of the receiving groove close to the placement rack.

[0024] Adopting the above solution has the following beneficial effects:

[0025] 1. In this solution, the test tubes are fixed by the load-bearing plate, which is suitable for the support needs of test tubes with different diameters and facilitates the centrifugation of exosomes in multiple test tubes at the same time.

[0026] 2. In this solution, compared with the prior art, the inside of the centrifugation chamber is shielded by the cover plate, which facilitates the sealing of the centrifugation chamber. At the same time, through the flipping action of the baffle plate, the interference to the surrounding test tubes during the removal of the test tubes is reduced, so as to improve the temperature stability of the surrounding test tubes after centrifugation.

[0027] 3. In this solution, through the comparison and control of the real-time temperature value, the stability of the temperature inside the centrifugation chamber is maintained, the temperature loss inside the centrifugation chamber is reduced, and the stability of the exosome structure during centrifugation and storage is ensured.

[0028] The additional aspects and advantages of the present invention will be partly given in the following description, partly will become obvious from the following description, or be understood through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 It is an axonometric view of an embodiment of the stem cell exosome extraction and storage device of the present invention;

[0030] Figure 2It is the top view of the embodiment of the stem cell exosome extraction and storage device of the present invention;

[0031] Figure 3 It is Figure 2 the schematic cross-sectional view taken along the A-A direction in

[0032] Figure 4 It is Figure 3 the enlarged schematic view of the partial B in

[0033] Figure 5 It is the schematic view of the centrifuge body in the embodiment of the stem cell exosome extraction and storage device of the present invention;

[0034] Figure 6 It is the schematic view of the placement rack in the embodiment of the stem cell exosome extraction and storage device of the present invention;

[0035] Figure 7 It is the schematic view of the cover plate in the embodiment of the stem cell exosome extraction and storage device of the present invention.

[0036] The reference numerals in the accompanying drawings of the specification include: 1, centrifuge body; 11, centrifugal chamber; 12, turntable; 13, central axis; 2, cover plate; 21, rotating plate; 22, shielding plate; 23, transfer pipe; 24, partition plate; 3, exchange port; 4, placement rack; 41, receiving groove; 42, clamping shaft; 43, pressure relief pipe; 44, load-bearing plate; 5, temperature sensor; 6, sliding groove. Detailed implementation manners

[0037] Next, the technical solutions of the present invention will be clearly and completely described in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0038] In the description of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the accompanying drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be construed as a limitation to the present invention. In addition, the terms "first", "second", "third" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance.

[0039] In the description of the present invention, it should be noted that unless otherwise clearly specified and limited, the terms "installation", "connection", and "coupling" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, and it can be the communication inside two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0040] The following is a further detailed description through specific embodiments:

[0041] Embodiment 1:

[0042] As shown in the Figures 1 to 7 accompanying drawings: A storage device for extracting and storing stem cell exosomes includes a centrifuge body 1 for ultracentrifuging stem cell exosomes. A centrifugation chamber 11 is opened in the centrifuge body 1, and a turntable 12 is rotatably fitted in the centrifugation chamber 11; a placement rack 4 for placing test tubes is provided on the turntable 12. The test tubes are used to hold exosome extraction liquid. Among them, the centrifuge body 1 is a prior art and will not be described in detail in this embodiment. A clamping mechanism for placing a plurality of test tubes is provided on the placement rack 4.

[0043] The clamping mechanism includes a clamping shaft 42 fixedly connected to the center of the bottom of the placement rack 4. A sliding groove 6 is opened at one end of the clamping shaft 42 away from the placement rack 4; a central shaft 13 is clamped in the sliding groove 6. In this embodiment, the sliding groove 6 communicates with an L-shaped groove, and a clamping block is slidably fitted in the L-shaped groove. The clamping block is fixedly connected to the central shaft 13, and when the central shaft 13 rotates clockwise, the clamping block abuts against the L-shaped groove; in another embodiment, the central shaft 13 and the clamping shaft 42 are fixedly connected by bolts, and the bolts are slidably fitted with the clamping shaft 42; the central shaft 13 is fixedly connected to the center of the turntable 12; a plurality of receiving grooves 41 are also fixedly connected to the bottom of the placement rack 4. The receiving grooves 41 are arranged in a ring centered on the clamping shaft 42, and the test tubes are located in the receiving grooves 41.

[0044] One end of the receiving groove 41 away from the placement rack 4 is higher than one end of the receiving groove 41 close to the placement rack 4; a receiving cavity is opened inside the receiving groove 41. The diameter of the receiving cavity at one end close to the placement rack 4 is larger than the diameter of the receiving cavity at one end away from the placement rack 4; a plurality of bearing plates 44 with different diameters are slidably fitted in the receiving cavity. A rubber ring (not shown in the figure) is fixedly connected to the center of the bearing plate 44, and the rubber ring abuts against the test tube.

[0045] A cold air delivery mechanism for refrigeration is provided on the inner wall of the centrifugation chamber 11, and a sealing mechanism for isolating a single test tube is provided at the top of the centrifugation chamber 11.

[0046] Among them, the cold air delivery mechanism includes an exchange port 3 communicated with the centrifugal chamber 11. The exchange port 3 is arranged in a ring centered on the centrifugal chamber 11. The exchange port 3 is communicated with a refrigerator for cooling through a trachea. The refrigerator is a prior art and will not be elaborated in this embodiment. The refrigerator is electrically connected to a control panel located inside the centrifuge body 1. A temperature sensor 5 for measuring the internal temperature of the centrifugal chamber 11 in real time is provided at the top of the centrifugal chamber 11. The temperature sensor 5 is electrically connected to the control panel, and the control panel is used to control the operation of the refrigerator based on the internal temperature of the centrifugal chamber 11.

[0047] The closing mechanism includes a cover plate 2 threadedly connected to the centrifuge body 1. A rotating plate 21 is slidably fitted at the center of the cover plate 2. The rotating plate 21 is located above the placement rack 4. A shielding plate 22 is rotatably fitted on the rotating plate 21. One side of the shielding plate 22 away from the center of the rotating plate 21 is rotatably fitted with the rotating plate 21. An isolation plate 24 is welded to the bottom of the rotating plate 21. The isolation plate 24 is arranged circumferentially centered on the shielding plate 22.

[0048] A gasket is provided between the clamping shaft 42 and the central shaft 13. A compression chamber is provided between the central shaft 13 and the clamping shaft 42. The compression chamber is communicated with the sliding groove 6. The compression chamber is communicated with a pressure relief pipe 43. A one-way valve and a solenoid valve are communicated between the pressure relief pipe 43 and the compression chamber. The one-way valve can only make the air in the pressure relief pipe 43 flow into the compression chamber. The solenoid valve is electrically connected to the control panel and is normally closed in the normal state. A transfer pipe 23 communicated with the pressure relief pipe 43 is opened on the isolation plate 24. The transfer pipe 23 and the pressure relief pipe 43 are located at the center of the centrifugal chamber 11. One end of the transfer pipe 23 away from the pressure relief pipe 43 is detachably connected with a plug (not shown in the figure). The plug is located on the side of the rotating plate 21 away from the isolation plate 24. When the placement rack 4 reaches the topmost point, the placement rack 4 abuts against the isolation plate 24. When the placement rack 4 moves to the lowest point, the placement rack 4 separates from the isolation plate 24.

[0049] The specific implementation process is as follows:

[0050] First, the cover plate 2 is detachably connected to the centrifuge body 1, so as to facilitate disassembly according to the needs of cleaning, maintenance, etc., expose the centrifugal chamber 11, and improve the flexibility of use; and the rotating plate 21 drives the baffle plate 22 and the isolation plate 24 to move, so as to facilitate the baffle plate 22 and the isolation plate 24 to correspond to the corresponding receiving grooves 41, and the baffle plate 22 is flipped to expose the receiving grooves 41 at the corresponding positions, so as to facilitate the replacement of the test tubes inside the receiving grooves 41, reduce the heat exchange between the inside of the centrifugal chamber 11 and the outside, and facilitate the maintenance of the temperature inside the centrifugal chamber 11. At the same time, during the opening process of the baffle plate 22, an isolation space is formed by the isolation plate 24 near the baffle plate 22, so as to reduce the diffusion of the outside air to the periphery of the centrifugal chamber 11, reduce the temperature influence on the surrounding test tubes during the process of taking out the test tubes, reduce the thermal stimulation to the adjacent test tubes, and ensure the stability of the centrifugal transfer and storage process of the exosome structure.

[0051] When installing the test tube into the inside of the receiving groove 41, the load-bearing plate 44 is clamped on the test tube. In this embodiment, two load-bearing plates 44 with inconsistent outer diameters can be used to clamp the upper and lower ends of the test tube, and then the test tube and the load-bearing plate 44 are both placed inside the receiving groove 41, so that the load-bearing plate 44 fills the gap between the test tube and the receiving groove 41.

[0052] At this time, the load-bearing plate 44 separates the space between the test tube and the receiving groove 41, and a relatively sealed space is formed between adjacent load-bearing plates 44. During the cooling process inside the centrifugal chamber 11, due to the characteristic that cold air sinks, the air pressure in the space remaining between adjacent load-bearing plates 44 will decrease after cooling, and the temperature at the top of the receiving groove 41 is higher than that at the lower part. The atmospheric pressure makes the load-bearing plate 44 move downward, and using the gradually shrinking receiving cavity, the clamping of the load-bearing plate 44 and the receiving cavity becomes more stable, improving the supporting effect of the load-bearing plate 44 on the test tube.

[0053] After the test tube is installed, the inclined receiving groove 41 separates the test tube from the direction of the centrifugal force, which is convenient for the generation of foam in the stem cell exosome and improves the separation effect. And through the isolation effect of the gasket, the air loss between the top of the clamping shaft 42 and the top of the central shaft 13 is reduced, so as to maintain the air inside the compression cavity.

[0054] Since the clamping shaft 42 is clamped with the central shaft 13, it is convenient for the turntable 12 to drive the placement rack 4 to rotate through the central shaft 13 and the clamping shaft 42, and drive the accommodating groove 41 to rotate through the turntable 12, so as to simultaneously centrifuge the exosomes in multiple test tubes, and it is convenient for different test tubes to be placed inside the accommodating groove 41. The temperature near the top of the centrifugation chamber 11 is measured by the temperature sensor 5 to judge whether the temperature inside the centrifugation chamber 11 reaches the predetermined cooling temperature, so as to ensure that the centrifugation is carried out at an appropriate temperature inside the centrifugation chamber 11, reduce the temperature difference between the upper and lower ends of the accommodating chamber, and facilitate the centrifugation of stem cell secretions.

[0055] Before centrifugation, the temperature inside the centrifugation chamber 11 is reduced by the cold air delivery mechanism. Since the inside of the compression chamber is sealed, as the temperature inside the centrifugation chamber 11 gradually decreases, the air sealed inside the compression chamber has a reduced air pressure due to the temperature decrease, and the original pressure cannot overcome the gravity of the placement rack 4, resulting in a gap between the placement rack 4 and the isolation plate 24, facilitating the air inside the centrifugation chamber 11 to enter the isolation plate 24, separating the placement rack 4 from the isolation plate 24, and facilitating the centrifugation.

[0056] After centrifugation is completed, by removing the plug, the transfer tube 23 is communicated with the pressure relief tube 43. Since the inside of the compression chamber is in a low-pressure state, normal outside air will enter the compression chamber through the restriction of the one-way valve. The increase in the temperature inside the compression chamber and the increase in outside gas cause the air pressure inside the compression chamber to increase, pushing the gap between the placement rack 4 and the isolation plate 24 to close, reducing the heat exchange between the air inside the isolation plate 24 and the surrounding test tubes during the opening process of the baffle 22, and ensuring the stability of the exosome structure in the surrounding test tubes.

[0057] Embodiment 2:

[0058] The difference from Embodiment 1 is that a transparent glass layer (not shown in the figure) is provided on the rotating plate 21.

[0059] The specific implementation process is as follows: During the process of rotating the rotating plate 21, the situation inside the centrifugation chamber 11 can be observed through the transparent glass layer, and at the same time, the positional relationship between the isolation plate 24 and the placement rack 4 is determined, facilitating the isolation plate 24 to block the test tubes inside the accommodating groove 41.

[0060] Embodiment 3:

[0061] The difference from Embodiment 2 is that the temperature sensor 5 is located on the isolation plate 24; the temperature sensor 5 is used to measure the real-time temperature value inside the isolation plate 24 in real time and send the real-time temperature value to the control panel; the control panel is used to compare the real-time temperature value with the set standard value. If the real-time temperature is greater than the standard value, a maintenance instruction is sent to the refrigerator; if the real-time temperature is less than or equal to the standard value, a stop instruction is sent to the refrigerator, and a centrifugation instruction is sent to the centrifuge body 1.

[0062] The control panel is further used to compare the real-time temperature value with the set warning value after the centrifuge body 1 finishes executing the centrifugation instruction. If the real-time temperature value is greater than the warning value, a start instruction is sent to the refrigerator, and an alarm instruction is sent to the centrifuge body 1; if the real-time temperature value is less than or equal to the warning value, a maintenance instruction is sent to the refrigerator.

[0063] For example, by comparing the real-time temperature value through the control panel, it is determined whether the temperature inside the centrifugation chamber 11 reaches the predetermined standard value to determine that the temperature inside the centrifugation chamber 11 reaches a suitable centrifugation treatment temperature; after centrifugation is completed, by comparing the real-time temperature value with the warning value, it is determined whether there is a long-term opening during the opening process of the current baffle 22 resulting in a temperature increase. By controlling the refrigerator to work, the inside of the centrifugation chamber 11 is maintained in a stable state, and by sending an alarm instruction to the centrifuge body 1 for reminder, so that the staff can timely close the baffle 22.

[0064] Obviously, the above embodiments are only examples given for clear illustration and are not limitations on the implementation manners. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation manners here. And the obvious changes or modifications derived therefrom are still within the protection scope of the present invention.

Claims

1. A stem cell exosome extraction and storage device, comprising a centrifuge body (1) for ultracentrifuging stem cell exosomes, wherein a centrifuge chamber (11) is provided in the centrifuge body (1), and a turntable (12) is rotatably provided in the centrifuge chamber (11); a placement rack (4) for placing a test tube is provided on the turntable (12), and the test tube is used to contain an exosome extract, wherein the device is characterized in that: The placement rack (4) is provided with a clamping mechanism for placing a plurality of test tubes; A cold air conveying mechanism for refrigeration is provided on the inner wall of the centrifugal chamber (11), and a sealing mechanism for isolating a single test tube is provided on the top of the centrifugal chamber (11).

2. The stem cell exosome extraction and storage device according to claim 1, characterized in that: The clamping mechanism comprises a clamping shaft (42) fixedly connected to the center of the bottom of the placement rack (4), and a sliding groove (6) is formed at one end of the clamping shaft (42) away from the placement rack (4); A central shaft (13) is clamped in the sliding groove (6), and the central shaft (13) is fixedly connected to the center of the rotating disk (12); a plurality of accommodating grooves (41) are also fixedly connected to the bottom of the placement rack (4), and the accommodating grooves (41) are arranged in a ring shape with the clamping shaft (42) as the center, and the test tubes are located in the accommodating grooves (41).

3. The stem cell exosome extraction and storage device according to claim 2, characterized in that: An accommodating cavity is formed inside the accommodating groove (41), and the diameter of the accommodating cavity at one end close to the placement rack (4) is larger than the diameter of the accommodating cavity at one end away from the placement rack (4); a plurality of bearing plates (44) of different diameters are slidably fitted in the accommodating cavity, and a rubber ring is fixedly connected at the center of the bearing plate (44), and the rubber ring abuts against the test tube.

4. The stem cell exosome extraction and storage device according to claim 3, characterized in that: The cold air delivery mechanism comprises an exchange port (3) connected to the centrifugal chamber (11), the exchange port (3) being arranged in a ring shape with the centrifugal chamber (11) as the center; the exchange port (3) is connected to a refrigerator for cooling through an air pipe; The refrigerator is electrically connected to a control panel, the control panel is located inside the centrifuge body (1), a temperature sensor (5) for measuring the internal temperature of the centrifugal chamber (11) in real time is fixedly connected to the top of the centrifugal chamber (11), the temperature sensor (5) is electrically connected to the control panel, and the control panel is used to control the operation of the refrigerator based on the internal temperature of the centrifugal chamber (11).

5. The stem cell exosome extraction and storage device according to claim 4, characterized in that: The sealing mechanism comprises a cover plate (2) detachably connected to the centrifuge body (1); a rotating plate (21) is slidably engaged at the center of the cover plate (2); the rotating plate (21) is located above the placement rack (4); and a shielding plate (22) is rotatably engaged on the rotating plate (21); The side of the shielding plate (22) away from the center of the rotating plate (21) is rotatably matched with the rotating plate (21), and the bottom of the rotating plate (21) is fixedly connected with an isolation plate (24), and the isolation plates (24) are arranged circumferentially with the shielding plate (22) as the center.

6. The stem cell exosome extraction and storage device according to claim 5, characterized in that: A compression chamber is provided between the central shaft (13) and the clamping shaft (42), the compression chamber is communicated with the sliding groove (6), the compression chamber is communicated with a pressure relief pipe (43), a one-way valve and a solenoid valve are communicated between the pressure relief pipe (43) and the compression chamber, the one-way valve can only allow the air in the pressure relief pipe (43) to flow into the compression chamber, the solenoid valve is electrically connected to the control panel, and the solenoid valve is in a normally closed state under normal conditions; A transfer pipe (23) in communication with the pressure relief pipe (43) is formed on the isolation plate (24), and the transfer pipe (23) and the pressure relief pipe (43) are located at the center of the centrifugal chamber (11); a plug is detachably connected to one end of the transfer pipe (23) away from the pressure relief pipe (43), and the plug is located on a side of the rotating plate (21) away from the isolation plate (24); When the placement rack (4) is moved to the topmost point, the placement rack (4) and the isolation plate (24) abut against each other; when the placement rack (4) moves to the lowest point, the placement rack (4) and the isolation plate (24) are separated.

7. The stem cell exosome extraction and storage device according to claim 6, characterized in that: A transparent glass layer is provided on the rotating plate (21).

8. The stem cell exosome extraction and storage device according to claim 7, characterized in that: The temperature sensor (5) is located on the isolation plate (24); The temperature sensor (5) is used to measure the real-time temperature value inside the isolation plate (24) in real time, and send the real-time temperature value to the control panel; the control panel is used to compare the real-time temperature value with a set standard value, and if the real-time temperature is greater than the standard value, send a maintenance instruction to the refrigerator; if the real-time temperature is less than or equal to the standard value, send a stop instruction to the refrigerator, and send a centrifugation instruction to the centrifuge body (1); The control panel is also used to compare the real-time temperature value with the set warning value after the centrifuge body (1) completes the execution of the centrifugation instruction; if the real-time temperature value is greater than the warning value, a start instruction is sent to the refrigerator, and an alarm instruction is sent to the centrifuge body (1); if the real-time temperature value is less than or equal to the warning value, a maintenance instruction is sent to the refrigerator.

9. The stem cell exosome extraction and storage device according to claim 8, characterized in that: A gasket is provided between the clamping shaft (42) and the central shaft (13).

10. The stem cell exosome extraction and storage device according to claim 9, characterized in that: An end of the accommodating groove (41) away from the placement rack (4) is higher than an end of the accommodating groove (41) close to the placement rack (4).

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