Nucleic acid detection device

Abstract

The invention relates to the technical field of nucleic acid detection, in particular to a nucleic acid detection device. The nucleic acid detection device comprises a body, the body comprises a sample processing part, an amplification part, a detection part, a first slide way, a micro-fluidic chip fixing part and a control part, the sample processing part, the amplification part and the detection part are sequentially arranged along the first slide way, the micro-fluidic chip fixing part is arranged on the first slide way in a sliding mode, and the control part is arranged on the micro-fluidic chip fixing part. The sample processing part comprises a magnetic control table, the amplification part comprises a heating piece, and the control part is respectively connected with the sample part, the amplification part, the detection part and the magnetic control table. The splitting decomposition and nucleic acid amplification of the sample are realized on the micro-fluidic chip, and an operator does not need to directly contact a reagent, so that the cross contamination of aerosol can be effectively avoided. The stability of nucleic acid amplification is improved; and the protection on operators is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid detection, and particularly to a nucleic acid detection device. Background Art

[0002] The methods and techniques for pathogen nucleic acid detection have also been continuously improved in this process. New technologies and new means have been continuously updated and iterated, all in order to quickly detect the results. However, traditional ordinary nucleic acid detection includes steps such as sample collection, sample inactivation, nucleic acid extraction and purification, nucleic acid amplification, and detection.

[0003] From collection to the final detection and amplification, each step urgently requires the participation of professionals. The requirements for the environment are also extremely high. And during this entire process, there are transfers among multiple people and multiple places, and during the biological sample processing, changing places and liquids multiple times are extremely likely to cause cross-contamination of aerosols, and also cause infections among many medical staff and testers. Furthermore, the demand for manpower will be greatly increased, resulting in a waste of human resources and an increase in labor costs. Moreover, the traditional detection process is time-consuming and cannot quickly produce results in a short time, greatly increasing the time cost. And most of the instruments on the market currently are nucleic acid amplification devices and do not contain biological sample purification devices. Therefore, an integrated nucleic acid detection device is the general trend. Summary of the Invention

[0004] Aiming at the technical problems of the prior art such as easy cross-infection, long detection time, and complex operation, the present invention provides a detection device and a detection method, which are simple to operate, easy to get started, and can be used by non-professionals. This device only needs to place the microfluidic chip used for detection on the body and operate the control part to know the detection and infection situation.

[0005] Aiming at the technical problems of the prior art such as easy cross-infection, long detection time, and complex operation, the present invention provides a detection device and a detection method, which are simple to operate, easy to get started, and can be used by non-professionals. This device only needs to place the microfluidic chip used for detection on the body and operate the control part to know the detection and infection situation.

[0006] In a first aspect of the present invention, a nucleic acid detection device is provided, including a body, the body includes a sample processing part, an amplification part, a detection part, a first slideway, a microfluidic chip fixing part, and a control part. The sample processing part, the amplification part, and the detection part are sequentially arranged along the first slideway. The microfluidic chip fixing part is slidably arranged on the first slideway. The sample processing part includes a magnetic control table, the amplification part includes a heating element, and the control part is respectively connected to the amplification part, the detection part, and the magnetic control table.

[0007] Preferably, the heating element is movably arranged in the amplification part.

[0008] Preferably, the detection unit has a microscope.

[0009] Preferably, the amplification unit includes a first power member, the first power member is connected to the heating member, and the first power member is electrically connected to the control unit.

[0010] Preferably, the amplification unit further includes a temperature sensing member, and the temperature sensing member is connected to the heating member or the control unit.

[0011] Preferably, an opening is provided in the middle of the microfluidic chip fixing part.

[0012] Preferably, a cover body is provided on the microfluidic chip fixing part.

[0013] Preferably, the nucleic acid detection device further includes a second power member and a belt, the second power member is connected to the belt, the second power member is electrically connected to the control unit, and the microfluidic chip fixing part is connected to the belt to drive the microfluidic chip fixing part to slide on the first slideway.

[0014] Preferably, the sample processing unit further includes a microfluidic chip, the microfluidic chip is arranged on the microfluidic chip fixing part, and the microfluidic chip is matched with the magnetic control table.

[0015] Preferably, the microfluidic chip is provided with a plurality of communicating chambers.

[0016] Preferably, the microfluidic chip is detachably arranged on the microfluidic chip fixing part.

[0017] Preferably, when the microfluidic chip is located in the detection unit, the heating member is located at the upper end and / or the lower end of the microfluidic chip.

[0018] Preferably, the nucleic acid detection device includes a third power member and a heat dissipation member, the heat dissipation member is slidably arranged on the first slideway, a second slideway is provided on the heat dissipation member, the microfluidic chip fixing part is slidably arranged on the second slideway, the third power member is respectively connected to the microfluidic chip fixing part and the control unit, and the third power member is used to drive the microfluidic chip fixing part to slide along the second slideway.

[0019] Preferably, the microscope is a fluorescence microscope.

[0020] Preferably, the microscope includes an objective lens, and the objective lens is movably arranged in the detection unit.

[0021] Preferably, the cover body is detachably connected to the microfluidic chip fixing part.

[0022] Preferably, the heat dissipation member is provided with heat dissipation holes.

[0023] Preferably, the number of the chambers is at least three.

[0024] Preferably, magnetic beads are provided in at least one of the chambers.

[0025] Preferably, a sample lysis solution is provided in at least one of the chambers.

[0026] Preferably, a detergent is provided in at least one of the chambers.

[0027] Preferably, an amplification agent is provided in at least one of the chambers.

[0028] Preferably, the second slideway is perpendicular to the first slideway.

[0029] Preferably, the detection unit includes a fourth power member, the fourth power member is connected to the objective lens, and the fourth power member is electrically connected to the control unit.

[0030] Preferably, the objective lens faces the microfluidic chip.

[0031] Preferably, a plurality of heat dissipation holes are provided on the heat dissipation member.

[0032] Preferably, an imaging element is provided in the detection unit.

[0033] Preferably, the detection unit further includes a filter member and a light source. The filter is provided on a side of the objective lens away from the microfluidic chip, and the light source is provided on a side of the filter member away from the objective lens.

[0034] Preferably, the imaging element is movably provided in the detection unit.

[0035] Preferably, the heat dissipation holes are uniformly provided on the heat dissipation member.

[0036] Preferably, the nucleic acid detection device further includes a wire protection member provided on the main body.

[0037] Preferably, the main body is further provided with an air outlet hole.

[0038] Preferably, the nucleic acid detection device further includes a switch provided on the main body.

[0039] Preferably, a plurality of filters are provided on the filter member.

[0040] Preferably, the filter member is rotatably provided in the detection unit.

[0041] Preferably, the filter member is circular.

[0042] Preferably, the nucleic acid detection device further includes a first limiting member for preventing the microfluidic chip fixing part from slipping off the first slideway.

[0043] Preferably, the nucleic acid detection device further includes a second limiting member for preventing the microfluidic chip fixing portion from slipping off the second slideway.

[0044] Preferably, the first limiting member is provided at one end of the first slideway close to the sample processing portion.

[0045] Preferably, the second limiting member is provided at one end of the second slideway away from the first slideway.

[0046] The second aspect of the present invention provides a method for nucleic acid detection, which uses the above-mentioned nucleic acid detection device for detection.

[0047] Preferably, it includes the following steps:

[0048] 1) Place the microfluidic chip containing the sample to be detected on the microfluidic chip fixing portion, and place the microfluidic chip fixing portion on the sample processing portion. Move the magnetic beads in the microfluidic chip through the magnetic control platform to complete the lysis, washing of the sample to be detected, and mixing with the amplification reagent;

[0049] 2) Move the microfluidic chip fixing portion along the first slideway to the amplification portion, and heat the microfluidic chip with the heating member to complete amplification;

[0050] 3) Move the microfluidic chip fixing portion along the first slideway to the detection portion for detection.

[0051] The present invention has at least one of the following beneficial effects:

[0052] 1) No cross - contamination: In the present invention, the lysis of the sample and nucleic acid amplification are both realized on the microfluidic chip, without the need for operators to directly contact the reagents, which can effectively avoid cross - contamination of aerosols. It improves the stability of nucleic acid amplification and increases the protection for operators.

[0053] 2) Fast: The sample processing and nucleic acid amplification are carried out in a short time until the final result appears, and the time consumed is extremely short. For example, the result appears within about 30 minutes.

[0054] 3) Reduced requirements for the experimental environment: When the nucleic acid detection device in the present invention detects nucleic acids, it no longer requires a professional PCR laboratory, and can be detected beside the sample collection site. There are no problems such as aerosols and cross - contamination, so the requirements for the experimental environment are greatly reduced.

[0055] 4) Integrated automation: When using the present invention, place the nucleic acid to be detected in the pre - prepared microfluidic chip filled with reagents, open the cover, put the microfluidic chip with the added sample inside, and then cover it with the lid. After that, only simple operations need to be carried out on the control part for internal automated operations. Description of the Drawings

[0056] Figure 1 This is a top view of the processing unit, amplification unit, and detection unit in a nucleic acid detection device of the present invention.

[0057] Figure 2 This is a longitudinal sectional view of the sample processing unit, amplification unit, and detection unit in the nucleic acid detection device of the present invention.

[0058] Figure 3 This is a front view of the nucleic acid detection device of the present invention.

[0059] Figure 4 This is a rear view of the nucleic acid detection device of the present invention.

[0060] Figure 5 This is a top view of the nucleic acid detection device of the present invention.

[0061] Figure 6 This is a schematic structural diagram of the microfluidic chip in the present invention.

[0062] Reference Signs:

[0063] 1 First Slideway

[0064] 2 Microfluidic Chip Fixing Part

[0065] 3 Control Unit

[0066] 4 Microfluidic Chip

[0067] 5 Magnetic Control Table

[0068] 6 Heating Element

[0069] 7 First Power Element

[0070] 8 Temperature Sensing Element

[0071] 9 Objective Lens

[0072] 10 Second Power Element

[0073] 11 Belt

[0074] 12 Cover

[0075] 13 Filter Element

[0076] 14 Cable Protection Element

[0077] 15 Air Outlet Hole

[0078] 16 Switch

[0079] 17 Heat Dissipation Element

[0080] 18 Heat Dissipation Hole

[0081] 19 First Limiting Element

[0082] 20 Second slideway

[0083] 21 Third power component

[0084] 22 Second limiting component

[0085] 23 Fourth power component Specific implementation manner

[0086] The following specific embodiments illustrate the implementation manners of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification.

[0087] Please refer to Figures 1 to 6 . It should be noted that the structures, ratios, sizes, etc. shown in the drawings of this specification are only used to cooperate with the content disclosed in the specification for those skilled in the art to understand and read, and are not used to limit the limited conditions under which the present invention can be implemented. Therefore, they do not have technical substantial significance. Any modification of the structure, change of the proportional relationship, or adjustment of the size, without affecting the effects that the present invention can produce and the purposes that can be achieved, should still fall within the scope covered by the technical content disclosed in the present invention. At the same time, the terms such as "upper", "lower", "left", "right", "middle", and "one" cited in this specification are only for the convenience of clear narration, and are not used to limit the scope under which the present invention can be implemented. The change or adjustment of their relative relationships, without substantial change in the technical content, should also be regarded as the scope under which the present invention can be implemented.

[0088] As Figures 1 - 6 shown, an embodiment of the present invention provides a nucleic acid detection device, including a main body, the main body includes a sample processing part, an amplification part, a detection part, a first slideway 1, a microfluidic chip fixing part 2, and a control part 3. The sample processing part, the amplification part, and the detection part are sequentially arranged along the first slideway 1. The microfluidic chip fixing part 2 is slidably arranged on the first slideway 1. The sample processing part includes a magnetic control table 5. The amplification part includes a heating element 6. The control part 3 is respectively connected to the amplification part, the detection part, and the magnetic control table 5.

[0089] When using the nucleic acid detection device in the present invention for detection, it includes the following steps: 1) Place the microfluidic chip 4 containing the sample to be detected on the microfluidic chip fixing part 2, and place the microfluidic chip fixing part 2 in the sample processing part. Move the magnetic beads in the microfluidic chip 4 through the magnetic control table 5 to complete the lysis, washing of the sample to be detected, and mixing with the amplification reagent; 2) Move the microfluidic chip fixing part 2 along the first slideway 1 to the amplification part, and heat the microfluidic chip 4 with the heating element 6 to complete the amplification; 3) Move the microfluidic chip fixing part 2 along the first slideway 1 to the detection part for detection.

[0090] In the present invention, the lysis of the sample and nucleic acid amplification are both realized on the microfluidic chip 4, without the need for the operator to directly contact the reagent, which can effectively avoid cross-contamination of aerosols. It improves the stability of nucleic acid amplification and increases the protection for the operator. The sample is processed and nucleic acid amplification is carried out within a short time until the final result appears, and the time consumed is extremely short. For example, the result appears within about 30 minutes. When the nucleic acid detection device in the present invention detects nucleic acid, it no longer requires a professional PCR laboratory, that is, it can be detected beside the sample collection site. There are no problems such as aerosols and cross-contamination, so the requirements for the experimental environment are greatly reduced. When using the present invention, the nucleic acid to be detected is placed in the pre-prepared microfluidic chip 4 filled with reagents, and the microfluidic chip 4 with the added sample is fixed to the microfluidic chip fixing part 2, and then only simple operations need to be performed on the control part 3 for internal automated operations.

[0091] In a preferred embodiment, an opening is provided in the middle of the microfluidic chip fixing part 2, and the opening is used to expose the microfluidic chip 4 for detection at the detection part. A cover body 12 is provided on the microfluidic chip fixing part 2, and the cover body 12 is detachably connected to the microfluidic chip fixing part 2.

[0092] In a preferred embodiment, the nucleic acid detection device further includes a second power member 10 and a belt 11. The second power member 10 is connected to the belt 11, and the second power member 10 is electrically connected to the control part. Further, the second power member 10 is a stepping motor, the stepping motor is connected to the belt 11 through a motor shaft, and the microfluidic chip fixing part 2 is connected to the belt 11 to drive the microfluidic chip fixing part 2 to slide on the first slideway 1.

[0093] In a specific embodiment, a slider is provided on one side of the microfluidic chip fixing part 2 close to the first slideway 1. The slider is arranged on the first slideway 1. The control part 3 is electrically connected to the second power member 10. The control part 3 controls the movement of the second power member 10, drives the microfluidic chip fixing part 2 connected to the belt 11 to move along the first slideway 1, and further controls the microfluidic chip fixing part 2 to move to the corresponding position on the first slideway 1 as needed.

[0094] In a preferred embodiment, the nucleic acid detection device further includes a microfluidic chip 4. The microfluidic chip 4 is provided with a plurality of connected chambers. The number of chambers is at least three. At least one chamber is provided with magnetic beads, at least one chamber is provided with a detergent, and the chamber close to the amplification part is provided with an amplification agent. The microfluidic chip 4 is detachably arranged on the microfluidic chip fixing part 2.

[0095] In a specific embodiment, the microfluidic chip 4 is provided with 4 connected chambers. During use, a sample lysate, a first washing solution, a second washing solution, and an amplification agent are sequentially filled along the direction of the chambers close to the detection part. The lysate can be a nucleic acid extraction agent, and the nucleic acid extraction reagent can rapidly lyse biological samples and retain the integrity of their nucleic acids. The lysed nucleic acids can specifically bind to magnetic beads. The biological sample and magnetic beads are placed in the chamber of the microfluidic chip 4 filled with the lysate, and then the fixing part 2 is placed in the sample processing part, and the cover body 12 is covered. By controlling the magnetic force between the magnetic control table 5 and the magnetic beads through the control part 3, the magnetic beads bound with nucleic acids can be controlled to pass through different chambers in sequence, so as to perform lysis in the lysate, wash impurities in the sample in the washing solution, and drag the washed magnetic beads to the chamber filled with the amplification agent.

[0096] In a preferred embodiment, when the microfluidic chip 4 is located in the amplification part, the heating element 6 is arranged at the upper end and / or the lower end of the microfluidic chip 4, and the heating element 6 is movably arranged in the amplification part.

[0097] In a preferred embodiment, the amplification part further includes a temperature sensing element 8, and the temperature sensing element 8 is connected to the heating element 6 and the control part 3. When the temperature sensing element 8 senses that the microfluidic chip 4 is heated to the time set by the control part 3, the heating element 6 stops heating the microfluidic chip 4.

[0098] Specifically, the amplification part includes a first power element 7, the first power element 7 is connected to the heating element 6, and the first power element 7 is electrically connected to the control part. Further, the first power element 7 is a stepping motor, and the stepping motor is connected to the heating element 6 through a motor shaft. When the microfluidic chip 4 moves from the sample processing part to the amplification part, the stepping motor drives the heating element 6 to approach the microfluidic chip 4 through the motor shaft, and the control part 3 controls the residence time of the microfluidic chip 4 in the amplification part. For example, a COVID-19 program can be input into the control part 3, corresponding time periods from reverse transcription to cDNA pre-denaturation to denaturation and annealing, and then the control part 3 controls the heating element 6 to perform heating amplification according to the program response.

[0099] More specifically, when the microfluidic chip 4 is located in the detection part, the heating element 6 is located directly below the microfluidic chip 4. When the microfluidic chip 4 needs to be heated, the stepping motor drives the heating element 6 to move upward through the motor shaft, and further drives the heating element 6 to approach the microfluidic chip 4 to realize the heating of the microfluidic chip 4.

[0100] Such as Figure 2As shown, in a preferred embodiment, the detection unit has a microscope, which is a fluorescence microscope. The microscope includes an objective lens 9, which is movably arranged in the detection unit and faces the microfluidic chip 4. The detection unit includes a fourth power member 23, which is connected to the objective lens 9 and electrically connected to the control unit. Further, the fourth power member 23 is a stepper motor, and the stepper motor is connected to the objective lens 9 through a motor shaft. When the microfluidic chip 4 moves to the detection unit, the stepper motor adjusts the position of the objective lens 9 and the microfluidic chip 4 through the motor shaft to achieve the detection of nucleic acid.

[0101] As Figure 2 shown, in a preferred embodiment, the detection unit further includes a filter member 13 and a light source. The filter is arranged on one side of the objective lens 9, and the light source is arranged on the side of the filter member 13 away from the objective lens 9. A number of filters are provided on the filter member 13, and the filter member 13 is rotatably arranged in the detection unit and is circular. The detection unit is provided with an imaging element, and the imaging element is a CCD. In use, the light source is turned on, and the filter member 13 is rotated as needed so that the filter member 13 transmits specific light. Then, the detection result of nucleic acid in the microfluidic chip 4 is observed through the eyepiece 9. At the same time, the imaging element performs imaging according to actual needs.

[0102] As Figure 1 shown, in a preferred embodiment, the nucleic acid detection device further includes a wire protection member 14, which is arranged on the main body and is used to protect the wires of the device.

[0103] As Figure 4 shown, in a preferred embodiment, the nucleic acid detection device further includes a switch 16, which is arranged on the main body.

[0104] As Figure 1As shown, in a preferred embodiment, it includes a third power component 21 and a heat dissipation component 17. A plurality of heat dissipation holes 18 are provided on the heat dissipation component 17, and the heat dissipation holes 18 are evenly arranged on the heat dissipation component 17. The heat dissipation component 17 is slidably arranged on the first slideway 1. A second slideway 20 is provided on one side of the heat dissipation component 17 away from the first slideway 1. The second slideway 20 is perpendicular to the first slideway 1. The microfluidic chip fixing part 2 is slidably arranged on the second slideway 20. The third power component 21 is electrically connected to the microfluidic chip fixing part 2, and the third power component 21 is electrically connected to the control part. The third power component 21 is arranged on the side of the heat dissipation component 17 where the second slideway 20 is provided. Further, the heat dissipation component 17 is a heat dissipation plate, and an opening is provided at a position corresponding to the microfluidic chip fixing part 2 on the heat dissipation plate to expose the microfluidic chip 4, thereby avoiding affecting the detection of nucleic acids.

[0105] Specifically, the heat dissipation holes 18 are provided on the heat dissipation plate, and the third power component 21 is a stepping motor, and the motor shaft of the stepping motor is connected to the microfluidic chip fixing part 2. There is some thermal steam in the nucleic acid after the heating amplification is completed, which will affect the detection and observation of it. In order to achieve better observation, when the heat dissipation component 17 drives the microfluidic chip fixing part 2 fixed with the microfluidic chip 4 into the detection part, the control part 3 controls the third power component 21 to drive the microfluidic chip fixing part 2 containing the nucleic acid to move along the direction away from the first slideway 1. The microfluidic chip fixing part 2 moves to the part of the heat dissipation component 17 where the heat dissipation holes 18 are provided, and the microfluidic chip 4 dissipates heat under the action of the heat dissipation holes 18. After the heat dissipation is completed, the control part controls the third power component 21 to drive the microfluidic chip fixing part 2 to move, and moves the microfluidic chip fixing part 2 containing the nucleic acid above the eyepiece 9 for detection and observation.

[0106] In a preferred embodiment, a slide rail is provided on one side of the microfluidic chip fixing part 2 close to the second slideway 20. The microfluidic chip fixing part 2 slides on the second slideway 20 through the slide rail, and the slide rail matches the second slideway 20. The matching means that the shape, size, and positional relationship correspond to each other.

[0107] As Figure 1As shown, in a preferred embodiment, the nucleic acid detection device further includes a first limiting member 19 and a second limiting member 22. The first limiting member 19 is provided at one end of the first slideway 1 close to the detection portion to prevent the microfluidic chip fixing portion 2 from slipping off the first slideway 1. The second limiting member 22 is provided at one end of the second slideway 20 away from the first slideway 1 to prevent the microfluidic chip fixing portion 2 from slipping off the second slideway 20. The first limiting member 19 and the second limiting member 22 belong to the prior art and can be, for example, stoppers, baffles, etc.

[0108] The control unit in the present invention can be a single-chip microcomputer, and the single-chip microcomputer can be an 8-bit minimum system. The control unit can select different brands and models, or a controller or processor with a higher number of bits, which belongs to the prior art.

[0109] The above embodiments are only illustrative of the principles and effects of the present invention and are not intended to limit the present invention. Any person familiar with this technology can modify or change the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or changes completed by those with ordinary knowledge in the technical field without departing from the spirit and technical idea disclosed by the present invention should still be covered by the claims of the present invention.

Claims

1. A nucleic acid detection device, characterized in that, Comprising a main body, the main body includes a sample processing section, an amplification section, a detection section, a first slideway (1), a microfluidic chip fixing section (2) and a control section (3), the sample processing section, the amplification section and the detection section are arranged in sequence along the first slideway (1), the microfluidic chip fixing section (2) is slidably arranged on the first slideway (1), the sample processing section includes a magnetic control table (5), the amplification section includes a heating element (6), and the control section (3) is respectively connected to the amplification section, the detection section and the magnetic control table (5).

2. The nucleic acid detection device according to claim 1, wherein The heating element (6) is movably arranged in the amplification section; and / or, the detection section has a microscope; and / or, the amplification section includes a first power element (7), the first power element (7) is connected to the heating element (6), and the first power element (7) is electrically connected to the control section; and / or, the amplification section further includes a temperature sensing element (8), the temperature sensing element (8) is connected to the heating element (6) or the control section (3); and / or, an opening is provided in the middle of the microfluidic chip fixing section (2); and / or, a cover body (12) is provided on the microfluidic chip fixing section (2); and / or, the nucleic acid detection device further includes a second power element (10) and a belt (11), the second power element (10) is connected to the belt (11), the second power element (10) is electrically connected to the control section, and the microfluidic chip fixing section (2) is connected to the belt (11) to drive the microfluidic chip fixing section (2) to slide on the first slideway (1); and / or, the sample processing section further includes a microfluidic chip (4), the microfluidic chip (4) is arranged on the microfluidic chip fixing section (2), and the microfluidic chip (4) matches the magnetic control table (5).

3. The nucleic acid detection device according to claim 2, wherein The microfluidic chip (4) is provided with a plurality of communicating chambers; and / or, the microfluidic chip (4) is detachably arranged on the microfluidic chip fixing section (2); and / or, when the microfluidic chip (4) is located in the detection section, the heating element (6) is located at the upper end and / or the lower end of the microfluidic chip (4); and / or, the nucleic acid detection device includes a third power element (21) and a heat dissipation element (17), the heat dissipation element (17) is slidably arranged on the first slideway (1), a second slideway (20) is provided on the heat dissipation element (17), the microfluidic chip fixing section (2) is slidably arranged on the second slideway (20), the third power element (21) is respectively connected to the microfluidic chip fixing section (2) and the control section (3), and the third power element (21) is used to drive the microfluidic chip fixing section (2) to slide along the second slideway (20); and / or, the microscope is a fluorescence microscope; and / or, the microscope includes an objective lens (9), and the objective lens (9) is movably arranged in the detection section; and / or, the cover body (12) is detachably connected to the microfluidic chip fixing section (2); and / or, heat dissipation holes (18) are provided on the heat dissipation element (17).

4. The nucleic acid detection device according to claim 3, wherein, The number of the chambers is at least three; and / or, magnetic beads are provided in at least one of the chambers; And / or, at least one of the chambers is provided with a sample lysis solution; And / or, at least one of the chambers is provided with a detergent; And / or, at least one of the chambers is provided with an amplification agent; And / or, the second slideway (20) is perpendicular to the first slideway (1); And / or, the detection unit includes a fourth power member (23), the fourth power member (23) is connected to the objective lens (9), and the fourth power member (23) is electrically connected to the control unit; And / or, the objective lens (9) faces the microfluidic chip (4); And / or, a plurality of heat dissipation holes (18) are provided on the heat dissipation member (17); And / or, the detection unit is provided with an imaging element; And / or, the detection unit further includes a filter member (13) and a light source. The filter is provided on a side of the objective lens (9) away from the microfluidic chip (4), and the light source is provided on a side of the filter member (13) away from the objective lens (9).

5. The nucleic acid detection device according to claim 4, wherein The imaging element is movably provided in the detection unit; And / or, the heat dissipation holes (18) are uniformly provided on the heat dissipation member (17); And / or, the nucleic acid detection device further includes a wire protection member (14), and the wire protection member (14) is provided on the main body; And / or, the main body is further provided with an air outlet hole (15); And / or, the nucleic acid detection device further includes a switch (16), and the switch (16) is provided on the main body; And / or, a plurality of filters are provided on the filter member (13); And / or, the filter member (13) is rotatably provided in the detection unit; And / or, the filter member (13) is circular; 6. The nucleic acid detection device according to any one of claims 1 to 5, characterized in that, The nucleic acid detection device further includes a first limiting member (19) for preventing the microfluidic chip fixing portion (2) from slipping off the first slideway (1); And / or, the nucleic acid detection device further includes a second limiting member (22) for preventing the microfluidic chip fixing portion (2) from slipping off the second slideway (20).

7. The nucleic acid detection device according to claim 6, wherein The first limiting member (19) is provided at one end of the first slideway (1) close to the sample processing portion.

8. The nucleic acid detection device according to claim 7, wherein The second limiting member (22) is provided at the second end of the slideway (20) away from the first slideway (1).

9. A method for nucleic acid detection, characterized in that, Detect using the nucleic acid detection device according to any one of claims 1-8.

10. The nucleic acid detection method according to claim 8, comprising the following steps: 1) Place the microfluidic chip (4) containing the sample to be detected on the microfluidic chip fixing portion (2), and place the microfluidic chip fixing portion (2) on the sample processing portion. Move the magnetic beads in the microfluidic chip (4) through the magnetic control table (5) to complete the lysis, washing of the sample to be detected, and mixing with the amplification agent. 2) Move the microfluidic chip fixing portion (2) along the first slideway (1) to the amplification portion, and heat the microfluidic chip (4) with the heating member (6) to complete amplification; 3) Move the microfluidic chip fixing portion (2) along the first slideway (1) to the detection portion for detection.

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