Fluorescence detection optical system and sample detection apparatus

Abstract

The invention provides a fluorescence detection optical system and a sample detection device, an excitation light source is arranged at the head end of an excitation channel, a light outlet is arranged at the tail end of the excitation channel, an excitation assembly is arranged between the excitation light source and the light outlet, and light emitted by each excitation light source can be transmitted to the light outlet through the excitation assembly; the emission module is perpendicular to the excitation module, the emission module and the excitation module surround the reaction tube, a collection assembly is installed between the detector and a light inlet of the emission module, and fluorescent light generated by a reagent in the reaction tube after being excited passes through the collection assembly and finally reaches the detector. The multiple excitation channels share one light outlet, the multiple collection channels share one light inlet, modularization and miniaturization of excitation and collection can be achieved, the situation that the size is large due to multiple inlets and outlets is avoided, the excitation module and the emission module are perpendicular to each other, the space can be fully utilized, miniaturization of the fluorescence detection optical system is facilitated, and the fluorescence detection optical system is convenient to use. And the production cost is reduced while the size is reduced.

Description

Technical Field

[0001] The present invention belongs to the technical field of molecular biology, and particularly relates to a fluorescence detection optical system and a sample detection device. Background Art

[0002] The PCR technology is a molecular biology technology for amplifying specific DNA fragments in vitro. Its greatest feature is that it can greatly increase trace amounts of DNA. The PCR technology has the characteristics of strong specificity, high sensitivity, low purity requirements, simplicity, and rapidity, and is thus widely used in molecular biology detection and analysis. In a PCR instrument or module, the fluorescence excitation and collection optical system is a very important part in addition to the temperature control system.

[0003] In existing multi-color PCR instruments, the optical path either adopts a linear scanning method combined with a patch LED, or a rotational scanning method combined with a patch LED and an optical fiber. Among them, in the method of linear scanning combined with a patch LED, the excitation and emission of each channel are usually integrated together. Although the excitation intensity is increased, moving parts and motors are introduced, which is not conducive to modularization and the cost is high. In the method of rotational scanning combined with a patch LED and an optical fiber, in addition to the optical fiber, there are also motors and conductive slip rings. It is suitable for a relatively large number of samples, occupies a large space, and the weight and cost are also high. Summary of the Invention

[0004] The main object of the present invention is to provide a fluorescence detection optical system and a sample detection device, aiming to solve the technical problem of the large volume and cost of the fluorescence detection optical system in the prior art.

[0005] To achieve the above object, the present invention provides a fluorescence detection optical system. The fluorescence detection optical system includes: an excitation module, including an excitation housing provided with a light outlet and a plurality of excitation channels. An excitation light source is provided at the head end of the excitation channel, the light outlet is provided at the tail end of the excitation channel, and an excitation component is installed between the excitation light source and the light outlet. The light emitted by each excitation light source can be transmitted to the light outlet through the excitation component; an emission module, perpendicular to the excitation module and enclosing an installation groove for accommodating a reaction tube with the excitation module. The emission module includes a collection housing provided with a light inlet and a plurality of collection channels. A detector is provided at the tail end of the collection channel, the light inlet is provided at the head end of the collection channel, and a collection component is installed between the detector and the light inlet. The fluorescence generated by the reagent in the reaction tube being excited passes through the light inlet and the collection component and finally reaches the detector.

[0006] In an embodiment of the present invention, the length direction of the excitation channel is consistent with the length direction of the excitation housing, and a plurality of the excitation channels are arranged in sequence along the width direction of the excitation housing, and a plurality of the excitation light sources are arranged alternately along the length direction of the excitation channel.

[0007] In an embodiment of the present invention, a plurality of the excitation channels include a main excitation channel and auxiliary excitation channels. The light outlet is arranged at the tail end of the main excitation channel, and an excitation focusing mirror is installed at the light outlet. The auxiliary excitation channels are located outside the main excitation channel along the width direction of the excitation housing. An excitation reflecting mirror is provided at the tail end of the auxiliary excitation channel located at the outermost side of the main excitation channel, and excitation dichroic mirrors are provided on the remaining auxiliary excitation channels and the main excitation channel.

[0008] In an embodiment of the present invention, the length of the main excitation channel is greater than the length of the auxiliary excitation channels, and the excitation light source on the main excitation channel is a main excitation light source, and the excitation light source on the auxiliary excitation channel is an auxiliary excitation light source. The main excitation light source protrudes away from the light outlet relative to the auxiliary excitation light source, and the light wavelengths of the auxiliary excitation light sources are different.

[0009] In an embodiment of the present invention, a plurality of the collection channels include a main collection channel and auxiliary collection channels. The light inlet is arranged at the head end of the main collection channel, and the main collection channel is correspondingly arranged with the main excitation channel. A collection reflecting mirror is provided at the tail end of the auxiliary collection channel located at the outermost side of the main collection channel, and collection dichroic mirrors are provided on the remaining auxiliary collection channels and the main collection channel.

[0010] In an embodiment of the present invention, the excitation assembly includes an excitation collimating mirror and an excitation filter. A pre-collimation space is formed between the excitation collimating mirror and the excitation light source, and the excitation filter is installed in the pre-collimation space.

[0011] In an embodiment of the present invention, the length direction of the collection channel is consistent with the length direction of the collection housing, and a plurality of the collection channels are arranged in sequence along the width direction of the collection housing, and a plurality of the detectors are arranged alternately along the length direction of the collection channel.

[0012] In an embodiment of the present invention, a collection collimating mirror is provided at the light inlet, and the collection assembly includes a pre-collection filter, a collection focusing mirror, and a post-collection filter that are sequentially arranged between the light inlet and the detector.

[0013] In an embodiment of the present invention, the excitation light source is a direct-insert LED; a pressing member for pressing the excitation assembly against the excitation housing is provided on the excitation housing; and / or, a pressing member for pressing the collection assembly against the collection housing is provided on the collection housing.

[0014] The present invention also provides a sample detection device, which includes the fluorescence detection optical system as described above.

[0015] By the above technical solutions, the fluorescence detection optical system provided by the embodiments of the present invention has the following beneficial effects:

[0016] When performing fluorescence detection using the fluorescence detection optical system, the reaction tube can be placed in the installation groove, and the mutually perpendicular excitation module and emission module are respectively arranged on both sides of the reaction tube. According to the fluorescence detection requirements, multiple excitation light sources can be controlled to emit light at different times. After the light emitted by the excitation light source passes through the excitation component, it can pass through the light outlet and enter the reaction cavity in the reaction tube. The fluorescence generated by the excitation of the reagent in the reaction cavity enters the emission module through the light inlet, and the light reaches the corresponding detector through the collection component of the corresponding collection channel. Then, the detector converts the optical signal into an electrical signal, thereby realizing fluorescence detection. Multiple collection channels can all collect light through the light inlet. In the present invention, multiple excitation channels share one light outlet, and multiple collection channels share one light inlet, which can realize the modularization and miniaturization of excitation and collection, avoid the large volume caused by multiple entrances and exits, and the excitation module and the emission module are perpendicular to each other, which can make full use of space, facilitate the miniaturization of the fluorescence detection optical system, reduce the volume and at the same time reduce the production cost.

[0017] Other features and advantages of the present invention will be described in detail in the subsequent specific implementation part. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The drawings are used to provide an understanding of the present invention and constitute a part of the specification, and are used to explain the present invention together with the following specific implementation manners, but do not constitute a limitation to the present invention. In the drawings:

[0019] Figure 1 is a schematic structural diagram of a fluorescence detection optical system according to an embodiment of the present invention;

[0020] Figure 2 is a partial schematic structural diagram of a fluorescence detection optical system according to an embodiment of the present invention;

[0021] Figure 3 is a schematic structural diagram of an excitation module of a fluorescence detection optical system according to an embodiment of the present invention;

[0022] Figure 4 is a schematic structural diagram of an emission module of a fluorescence detection optical system according to an embodiment of the present invention;

[0023] Figure 5 is a schematic diagram of the optical path principle of an excitation module of a fluorescence detection optical system according to an embodiment of the present invention;

[0024] Figure 6 It is a schematic diagram of the optical path principle of the emission module of the fluorescence detection optical system according to an embodiment of the present invention.

[0025] Description of the reference numerals

[0026] Label Name Label Name

[0027] 100 Fluorescence detection optical system 2 Emission module

[0028] 1 Excitation module 21 Collection housing

[0029] 11 Excitation housing 211 Light inlet

[0030] 111 Light outlet 212 Collection channel

[0031] 112 Excitation channel 212a Main collection channel

[0032] 112a Main excitation channel 212b Secondary collection channel

[0033] 112b Secondary excitation channel 22 Detector

[0034] 12 Excitation light source 23 Collection assembly

[0035] 12a Main excitation light source 231 Collection dichroic mirror

[0036] 12b Secondary excitation light source 232 Collection reflector

[0037] 13 Excitation assembly 233 Collection collimator

[0038] 131 Excitation focusing mirror 234 Collection pre-filter

[0039] 132 Excitation dichroic mirror 235 Collection focusing mirror

[0040] 133 Excitation reflector 236 Collection post-filter

[0041] 134 Excitation collimator 3 Mounting groove

[0042] 135 Excitation filter 200 Reaction tube

[0043] 14 Pre-collimation space Detailed implementation manners

[0044] The following describes in detail the specific embodiments of the present invention with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are only for the purpose of illustration and explanation of the present invention, and are not used to limit the present invention.

[0045] The following describes the fluorescence detection optical system according to the present invention with reference to the accompanying drawings.

[0046] like Figures 1 to 6 As shown, in an embodiment of the present invention, the fluorescence detection optical system 100 includes an excitation module 1 and an emission module 2, the excitation module 1 includes an excitation shell 11 with a light outlet 111 and a plurality of excitation channels 112, an excitation light source 12 is provided at the head end of the excitation channel 112, the light outlet 111 is provided at the tail end of the excitation channel 112, an excitation component 13 is installed between the excitation light source 12 and the light outlet 111, and the light emitted by each excitation light source 12 can be transmitted to the light outlet 111 through the excitation component 13; the emission module 2 is perpendicular to the excitation module 1 and forms a mounting groove 3 for accommodating the reaction tube 200 with the excitation module 1. The emission module 2 includes a collection shell 21 with a light inlet 211 and a plurality of collection channels 212. A detector 22 is provided at the tail end of the collection channel 212. The light inlet 211 is provided at the head end of the collection channel 212. A collection component 23 is installed between the detector 22 and the light inlet 211. The fluorescence generated by the reagent in the reaction tube 200 when stimulated passes through the light inlet 211 and the collection component 23 and finally reaches the detector 22. It should be noted that in the embodiment of the present invention, the reagent in the reaction tube 200 includes a sample and a fluorescent reagent.

[0047] It should be noted that multiple excitation channels 112 share a light outlet 111, and multiple collection channels 212 share a light inlet 211. The head end of the excitation channel 112 is the front end of the light path, the tail end of the excitation channel 112 is the rear end of the light path, the head end of the collection channel 212 is the front end of the light path, and the tail end of the collection channel 212 is the rear end of the light path. The light propagates along the light path in the front-to-back direction. The fluorescence detection optical system 100 in this embodiment is mainly used for reagent detection in the reaction tube 200 in the sample detection equipment, and the reaction tube 200 can be a PCR flat tube in the prior art. The number of excitation channels 112 and collection channels 212 is consistent and one-to-one corresponding, the excitation light source 12 can emit light of different wavelengths, and under the action of the excitation component 13, the light in multiple excitation channels 112 can pass through the light outlet 111. In one embodiment, the number of excitation channels 112 and collection channels 212 is 4, and in other embodiments, the number of excitation channels 112 and collection channels 212 can be set according to actual use requirements. The detector 22 is one of a light-to-voltage converter, a silicon photodiode, and a silicon photomultiplier tube, and can convert an optical signal into an electrical signal.

[0048] When performing fluorescence detection using the fluorescence detection optical system 100 in this embodiment, the reaction tube 200 can be placed in the installation groove 3. The excitation module 1 and the emission module 2 that are perpendicular to each other are respectively arranged on both sides of the reaction tube 200. According to the fluorescence detection requirements, multiple excitation light sources 12 can be controlled to emit light at different times. After the light emitted by the excitation light source 12 passes through the excitation component 13, it passes through the light outlet 111 and enters the reaction chamber in the reaction tube 200, exciting the reagent in the reaction chamber to generate fluorescence. The fluorescence enters the emission module 2 through the light inlet 211, and after passing through the collection component 23 of the corresponding collection channel 212, it reaches the corresponding detector 22. The detector 22 can convert the optical signal into an electrical signal, thereby realizing fluorescence detection. Multiple collection channels 212 can all collect light through the light inlet 211. In this embodiment, multiple excitation channels 112 share one light outlet 111, and multiple collection channels 212 share one light inlet 211, which can realize the modularization and miniaturization of excitation and collection, avoid the situation of large volume caused by multiple entrances and exits, and the excitation module 1 and the emission module 2 are perpendicular to each other, which can make full use of space, facilitate the miniaturization of the fluorescence detection optical system 100, reduce the volume and at the same time reduce the production cost.

[0049] As Figures 3 to 5 shown, the length direction of the excitation channel 112 is the same as the length direction of the excitation shell 11. Multiple excitation channels 112 are arranged in sequence along the width direction of the excitation shell 11, and multiple excitation light sources 12 are arranged staggered with each other along the length direction of the excitation channel 112. The excitation light source 12 and the light outlet 111 are not collinear in the front-back direction. The excitation channel 112 extends integrally along the length direction of the excitation shell 11, and multiple excitation channels 112 are arranged in sequence along the up-down direction. The excitation channel 112 can include a straight excitation part at the front end and a bent excitation part communicated with the straight excitation part. The excitation light source 12 is located at the front end of the straight excitation part. Figure 3 and Figure 5 the number of excitation channels 112 in

[0050] Specifically, in one embodiment, the multiple excitation channels 112 include a main excitation channel 112a and auxiliary excitation channels 112b. The light output port 111 is provided at the tail end of the main excitation channel 112a, and an excitation focusing mirror 131 is installed at the light output port 111. The auxiliary excitation channels 112b are located outside the main excitation channel 112a along the width direction of the excitation housing 11. At the tail ends of the auxiliary excitation channels 112b located at the outermost sides above and below the main excitation channel 112a, excitation reflectors 133 are provided. Dichroic mirrors 132 are provided on the remaining auxiliary excitation channels 112b and the main excitation channel 112a. In other embodiments, the excitation reflectors 133 can be replaced with dichroic mirrors, or one side can use a dichroic mirror and the other side can use an excitation reflector 133.

[0051] It should be noted that the length of the main excitation channel 112a is greater than that of the auxiliary excitation channels 112b, and the excitation light source 12 on the main excitation channel 112a is the main excitation light source 12a, and the excitation light source 12 on the auxiliary excitation channels 112b is the auxiliary excitation light source 12b. The main excitation light source 12a protrudes away from the light output port 111 relative to the auxiliary excitation light source 12b, and the light wavelengths of the auxiliary excitation light sources 12b are different. As Figure 5 shown, the third excitation channel 112 from top to bottom is the main excitation channel 112a, and the main excitation light source 12a can emit white light. The four excitation channels 112 can emit blue light, green light, white light, and red light respectively.

[0052] In one embodiment, as Figure 4 and Figure 6 shown, the multiple collection channels 212 include a main collection channel 212a and auxiliary collection channels 212b. The light input port 211 is provided at the head end of the main collection channel 212a, and the main collection channel 212a is correspondingly arranged with the main excitation channel 112a. Collection reflectors 232 are provided at the tail ends of the auxiliary collection channels 212b located at the outermost sides above and below the main collection channel 212a. Dichroic mirrors 231 are provided on the remaining auxiliary collection channels 212b and the main collection channel 212a. Collection reflectors 232 are provided on the uppermost and lowermost auxiliary collection channels 212b, which can reflect light to the main collection channel 212a. In other embodiments, the collection reflectors 232 can be replaced with dichroic mirrors, or one side can use a dichroic mirror and the other side can use a collection reflector 232.

[0053] In one embodiment, the excitation component 13 includes an excitation collimating mirror 134 and an excitation filter 135. A pre-collimation space 14 is formed between the excitation collimating mirror 134 and the excitation light source 12, and the excitation filter 135 is installed in the pre-collimation space 14. The excitation collimating mirror 134 in this embodiment can be a double-convex spherical mirror. The excitation light source 12 is located at the focal plane position of the excitation collimating mirror 134. Installing the excitation filter 135 in the pre-collimation space 14 between the excitation collimating mirror 134 and the excitation light source 12, different from installing the excitation filter 135 behind the excitation collimating mirror 134, can make full use of the installation space and reduce the length of the excitation channel 112.

[0054] In the embodiment of the present invention, the length direction of the collection channel 212 is the same as the length direction of the collection housing 21. A plurality of collection channels 212 are arranged in sequence along the width direction of the collection housing, and a plurality of detectors 22 are arranged staggeredly along the length direction of the collection channel 212. The collection channel 212 in this embodiment may include a straight collection portion at the front end and a bent collection portion communicating with the straight collection portion. The collection light source is located at the front end of the straight collection portion. The light in the straight collection portion can enter the main collection channel 212a through the reflection of the bent collection portion. The detectors 22 are arranged staggeredly in the front-back direction, and the detectors 22 are not collinear with the light inlet 21 in the front-back direction, which can improve the structural compactness of the emission module 2.

[0055] Specifically, a collection collimating mirror 233 is provided at the light inlet 211. The collection component 23 includes a pre-collection filter 234, a collection focusing mirror 235, and a post-collection filter 236 arranged in sequence between the light inlet 211 and the detector 22. The collection collimating mirror 233 in this embodiment is a plano-convex spherical mirror, which collimates the light at the light inlet 211. The collection focusing mirror 235 is a double-convex spherical mirror, which can ensure that the detector 22 collects enough light. At the same time, combined with double filtering processing, the detection dynamic range of the fluorescence detection optical system 100 is improved. In one embodiment, the collection collimating mirror 233 can be made of organic glass (PMMA) or one of the EP6000 components, and the collection focusing mirror 235 can be made of organic glass (PMMA) or one of the EP6000 components.

[0056] In one embodiment, four excitation channels 112 are integrated together to form an excitation module 1, and the direct-insertion LED of each excitation channel 112 is a single F5 direct-insertion LED with high brightness and small divergence angle. Specifically, the excitation channel 112 located at the top is a FAM channel, and the blue light emitted by the single F5 direct-insertion LED is first filtered by two short-wave pass excitation filters 135 (S495), then collimated by a double convex spherical mirror P1, reflected by an excitation reflector 133, passed through an excitation dichroic mirror 132 (S515), reflected by an excitation dichroic mirror 132 (L580), and finally focused to the center of the reaction chamber of the reaction tube 200 by a cylindrical mirror P2. The excitation channel 112 located in the second row is the VIC channel. The green light emitted by a single F5 plug-in LED is first filtered by a long-wave pass excitation filter 135 (L510) and a short-wave pass excitation filter 135 (S540), then collimated by a double convex spherical mirror P1, reflected by an excitation dichroic mirror 132 (S515), and then reflected by an excitation dichroic mirror 132 (L580), and finally focused by a cylindrical mirror P2 to the center of the reaction chamber of the reaction tube 200. The excitation channel 112 located in the third row is the ROX channel. The white light emitted by a single F5 plug-in LED is first filtered by a long-wave pass excitation filter 135 (L565) and a short-wave pass excitation filter 135 (S590), then collimated by a double convex spherical mirror P1, passed through an excitation dichroic mirror 132 (S620) and an excitation dichroic mirror 132 (L580), and finally focused by a cylindrical mirror P2 to the center of the reaction chamber of the reaction tube 200. The excitation channel 112 located in the fourth row is the Cy5 channel. The red light emitted by a single F5 plug-in LED is first filtered by a long-wave pass excitation filter 135 (L630) and a short-wave pass excitation filter 135 (S650), then collimated by a double convex spherical mirror P1, reflected by an excitation dichroic mirror 132 (L685), and then reflected by an excitation dichroic mirror 132 (S620), passing through the excitation dichroic mirror 132 (L580), and finally focused by a cylindrical mirror P2 to the center of the reaction chamber of the reaction tube 200.

[0057] The LEDs of the four excitation channels 112 in this embodiment can be lit up in time, and the cut-off depth outside the passband of each excitation filter 135 is above OD4. For example, the wavelength corresponding to the spectral transmittance of the short-wave pass excitation filter 135 (S495) is 495nm when the spectral transmittance is 50%, Tavg≥90%@400~490nm, and the cut-off depth≥OD4@510~800nm, and the rest can be deduced by analogy. Among them, the excitation dichroic mirror 132 (L685) can be replaced by an excitation reflector, and the excitation dichroic mirror 132 can use dichroic mirrors S515, S620 and L580 designed with a 0° incident angle, or a dichroic mirror specially designed with a 30° incident angle, to improve the utilization rate of light energy.

[0058] In one embodiment, the excitation light source 12 is a through-hole LED; using different through-hole LEDs for each excitation channel 112 can ensure the luminous intensity and enable time-division lighting. In this embodiment, a pressing member for pressing the excitation component 13 against the excitation housing 11 is provided on the excitation housing 11; moreover, a pressing member for pressing the collection component 23 against the collection housing 21 is provided on the collection housing 21, making the performance more stable and reliable.

[0059] Specifically, the excitation housing 11 adopts a split design. The excitation housing 11 includes an upper cover and a housing body. A clamping groove for positioning the excitation component 13 is provided on the housing body. The pressing member is a black single-sided adhesive pasted inside the upper cover, which can press the upper cover and the housing body, and press the excitation component 13 between the upper cover and the housing body against the excitation housing 11. The black single-sided adhesive can be provided on each excitation channel 112. The excitation component 13 can be prevented from shaking when pressed by the black single-sided adhesive inside the upper cover; the collection housing 21 in this embodiment also adopts a split design and can include an upper cover and a housing body. A clamping groove for positioning the collection component 23 is provided on the housing body. The pressing member can be a black single-sided adhesive pasted inside the upper cover, which can press the collection component 23 between the upper cover and the housing body against the collection housing 21. The black single-sided adhesive can be provided on each collection channel 212. The collection component 23 can be prevented from shaking when pressed by the black single-sided adhesive inside the upper cover.

[0060] In another embodiment, the excitation housing 11 adopts a split design. The excitation housing 11 includes an upper cover and a housing body. The upper cover adopts a two-color molding process. The pressing member is a diaphragm on the upper cover. The excitation component 13 is pressed against by the diaphragm on the upper cover inside the excitation housing 11, which can prevent shaking; the collection housing 21 adopts a split design and can include an upper cover and a housing body. The upper cover adopts a two-color molding process. The pressing member is a diaphragm on the upper cover. The collection component 23 is pressed against by the diaphragm on the upper cover inside the collection housing 21, which can prevent shaking, making the performance more stable and reliable.

[0061] Such as Figure 6As shown, in another embodiment, four collection channels 212 are integrated together to form a transmission module 2. The collection channel 212 at the lowermost end is the FAM channel. The fluorescence generated by the excitation of the reagent in the reaction tube 200 is first collimated by the plano-convex spherical mirror P3. Part of the light passes through the collection dichroic mirror 231 (S620), is reflected by the collection dichroic mirror 231 (L580), and then is reflected by the collection mirror 232. After being filtered by the pre-collection filter 234 (L510) and the pre-collection filter 234 (S540), it is focused by the biconvex spherical mirror P4 and filtered by the absorption-type post-collection filter 236 (JB490), and then is gathered onto the detector 22. The detector 22 converts the optical signal into an electrical signal. The collection channel 212 in the third row from top to bottom is the VIC channel. The fluorescence generated by the excitation of the reagent in the reaction tube 200 is first collimated by the plano-convex spherical mirror P3. Part of the light passes through the collection dichroic mirror 231 (S620) and the collection dichroic mirror 231 (L580), is filtered by the pre-collection filter 234 (L555) and the pre-collection filter 234 (S580), and then is focused by the biconvex spherical mirror P4 and filtered by the absorption-type post-collection filter 236 (CB550), and then is gathered onto the detector 22. The collection channel 212 in the second row from top to bottom is the ROX channel. The fluorescence generated by the excitation of the reagent in the reaction tube 200 is first collimated by the plano-convex spherical mirror P3. Part of the light is reflected by the collection dichroic mirror 231 (S620) and then is reflected by the collection dichroic mirror 231 (L695). After being filtered by the pre-collection filter 234 (L605) and the pre-collection filter 234 (S650), it is focused by the biconvex spherical mirror P4 and filtered by the absorption-type post-collection filter 236 (CB590), and then is focused onto the detector 22. The collection channel 212 in the first row from top to bottom is the Cy5 channel. The fluorescence generated by the excitation of the reagent in the reaction tube 200 is first collimated by the plano-convex spherical mirror P3. Part of the light is reflected by the collection dichroic mirror 231 (S620), passes through the collection dichroic mirror 231 (L695), is reflected by the collection dichroic mirror 231 (S620), is filtered by the pre-collection filter 234 (L670) and the pre-collection filter 234 (S755), and then is focused by the biconvex spherical mirror P4 and filtered by the absorption-type post-collection filter 236 (HB640), and then is focused onto the detector 22.

[0062] In one embodiment, the excitation light sources for the FAM channel and the ROX channel are LED lamps with a luminous power greater than 11 mW at 20 mA; the excitation light sources for the VIC channel and the Cy5 channel are LED lamps with a luminous power greater than 6.5 mW at 20 mA.

[0063] When the LED light in the FAM channel is lit, only the signal of the FAM channel is collected, and so on for the rest. Among them, the dichroic mirror 231 (S620) for collection can be replaced by the collection mirror 232. The dichroic mirror 231 for collection can adopt dichroic mirrors S620, L580, and L695 designed with an incident angle of 0°, or dichroic mirrors specially designed with an incident angle of 30° to improve the utilization rate of light energy.

[0064] In one embodiment, the absorption type post-collection filter 236 can adopt long-pass filters with models JB490, CB550, CB590, and HB640. The wavelengths corresponding to a spectral transmittance of 50% are 490nm, 550nm, 590nm, and 640nm respectively. It can filter out the excitation light components cleanly and has a low cost. The absorption type filter can also be replaced by a band-pass filter. The filter combinations of each channel are only for display, and other filter combinations are not excluded in order to pursue lower crosstalk.

[0065] The present invention also proposes a sample detection device. The sample detection device includes the fluorescence detection optical system 100 as described above, and the specific structure of the fluorescence detection optical system 100 refers to the above embodiments. Since the sample detection device adopts all the technical solutions of the above all embodiments, it has at least all the beneficial effects brought by the technical solutions of the above embodiments, which will not be elaborated one by one here. The sample detection device in this embodiment is mainly used for nucleic acid detection.

[0066] In the description of the present invention, it should be understood that the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include at least one of these features. In the description of the present invention, the meaning of "a plurality" is at least two, such as two, three, etc., unless otherwise specifically defined.

[0067] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed", etc. should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or integrated; it can be a mechanical connection, an electrical connection, or communicable with each other; it can be directly connected, or indirectly connected through an intermediate medium, and can be the internal communication of two components or the interaction relationship between two components, unless otherwise clearly limited. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0068] In the description of this specification, the descriptions referring to terms such as "one embodiment", "some embodiments", "examples", "specific examples", or "some examples", etc. mean that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, without contradiction, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples.

[0069] Although the embodiments of the present invention have been shown and described above, it can be understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those of ordinary skill in the art can make changes, modifications, substitutions, and variations to the above embodiments within the scope of the present invention.

Claims

1. A fluorescence detection optical system, characterized in that, The fluorescence detection optical system (100) includes: An excitation module (1), including an excitation housing (11) provided with a light outlet (111) and a plurality of excitation channels (112). The head end of each excitation channel (112) is provided with an excitation light source (12), the light outlet (111) is arranged at the tail end of the excitation channel (112), and an excitation component (13) is installed between the excitation light source (12) and the light outlet (111). The light emitted by each excitation light source (12) can propagate through the excitation component (13) to the light outlet (111); An emission module (2), perpendicular to the excitation module (1) and enclosing an installation groove (3) for accommodating a reaction tube (200) with the excitation module (1). The emission module (2) includes a collection housing (21) provided with a light inlet (211) and a plurality of collection channels (212). The tail end of each collection channel (212) is provided with a detector (22), the light inlet (211) is arranged at the head end of the collection channel (212), and a collection component (23) is installed between the detector (22) and the light inlet (211). The fluorescence generated by the excitation of the reagent in the reaction tube (200) passes through the light inlet (211) and the collection component (23) in sequence, and finally reaches the detector (22).

2. The fluorescence detection optical system according to claim 1, wherein The length direction of the excitation channel (112) is consistent with the length direction of the excitation housing (11). A plurality of the excitation channels (112) are arranged in sequence along the width direction of the excitation housing (11), and a plurality of the excitation light sources (12) are arranged staggered along the length direction of the excitation channel (112).

3. The fluorescence detection optical system according to claim 2, wherein, A plurality of the excitation channels (112) include an excitation main channel (112a) and an excitation sub-channel (112b). The light outlet (111) is arranged at the tail end of the excitation main channel (112a), and an excitation focusing mirror (131) is installed at the light outlet (111). The excitation sub-channel (112b) is located outside the excitation main channel (112a) along the width direction of the excitation housing (11). An excitation reflecting mirror (133) is provided at the tail end of the excitation sub-channel (112b) located at the outermost side of the excitation main channel (112a), and excitation dichroic mirrors (132) are provided on the remaining excitation sub-channels (112b) and the excitation main channel (112a).

4. The fluorescence detection optical system according to claim 3, characterized in that, The length of the excitation main channel (112a) is greater than the length of the excitation sub-channel (112b). The excitation light source (12) on the excitation main channel (112a) is an excitation main light source (12a), and the excitation light source (12) on the excitation sub-channel (112b) is an excitation sub-light source (12b). The excitation main light source (12a) protrudes away from the light outlet (111) relative to the excitation sub-light source (12b), and the light wavelengths of the excitation sub-light sources (12b) are different.

5. The fluorescence detection optical system according to claim 3, wherein A plurality of the collection channels (212) include a main collection channel (212a) and auxiliary collection channels (212b). The light incident port (211) is disposed at the head end of the main collection channel (212a), and the main collection channel (212a) and the main excitation channel (112a) are correspondingly arranged. A collection reflector (232) is provided at the tail end of the outermost auxiliary collection channel (212b) of the main collection channel (212a), and dichroic mirrors (231) are provided on the remaining auxiliary collection channels (212b) and the main collection channel (212a).

6. The fluorescence detection optical system according to any one of claims 1 to 5, characterized in that The excitation assembly (13) includes an excitation collimating mirror (134) and an excitation filter (135). A pre-collimation space (14) is formed between the excitation collimating mirror (134) and the excitation light source (12), and the excitation filter (135) is installed in the pre-collimation space (14).

7. The fluorescence detection optical system according to any one of claims 1 to 5, characterized in that, The length direction of the collection channel (212) is consistent with the length direction of the collection housing (21). A plurality of the collection channels (212) are arranged in sequence along the width direction of the collection housing (21), and a plurality of the detectors (22) are arranged staggered with each other along the length direction of the collection channel (212).

8. The fluorescence detection optical system according to any one of claims 1 to 5, characterized in that A collection collimating mirror (233) is provided at the light incident port (211). The collection assembly (23) includes a pre-collection filter (234), a collection focusing mirror (235), and a post-collection filter (236) which are sequentially arranged between the light incident port (211) and the detector (22).

9. The fluorescence detection optical system according to any one of claims 1 to 5, characterized in that The excitation light source (12) is a direct plug-in LED; and / or A pressing member for pressing the excitation assembly (13) against the excitation housing (11) is provided on the excitation housing (11); and / or A pressing member for pressing the collection assembly (23) against the collection housing (21) is provided on the collection housing (21).

10. A sample detection device, characterized in that, The sample detection device includes the fluorescence detection optical system (100) according to any one of claims 1 to 9.

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