Sample detection device and sample detection method

Abstract

The invention discloses a sample detection device and a sample detection method, in the sample detection device, a detection module is used for carrying out flow fluorescence detection treatment on a sample to enable the sample to emit a first light beam, or is used for carrying out chemiluminescence detection treatment on the sample to enable the sample to emit a second light beam; the sensing module is used for receiving the first light beam or the second light beam; the first circuit is used for converting the received electric signal into an analog signal; the second circuit is used for converting the received electric signal into a digital signal; the control module is used for controlling the switching module to be connected with the first circuit when the detection mode of the sample is flow fluorescence detection, and processing the sample based on the analog signal to obtain a detection result of the flow fluorescence detection; and when the detection mode of the sample is chemiluminescence detection, controlling the switching module to be connected with the second circuit, and processing the sample based on the digital signal to obtain a detection result of chemiluminescence detection. Based on the mode, the reliability of the sample detection device can be improved.

Description

Technical Field

[0001] This application relates to the field of detection technology, and particularly to a sample detection device and a sample detection method. Background Art

[0002] In the prior art, when performing sample detections in different ways, such as flow cytometry fluorescence detection and chemiluminescence detection, due to the different detection conditions corresponding to different ways, for example, the processing methods of the signals obtained by photoelectric conversion are different, different sample detection devices are usually used for sample detection.

[0003] The defect of the prior art is that due to the different characteristics of flow cytometry fluorescence detection and chemiluminescence detection, for example, flow cytometry fluorescence detection has the advantages of fast detection speed and low cost, while chemiluminescence detection has the advantages of high detection sensitivity and wide linear range. Any existing sample detection device cannot simultaneously have the ability to perform flow cytometry fluorescence detection and chemiluminescence detection, resulting in the inability of existing sample detection devices to simultaneously possess the above two advantages. Moreover, setting up two sample detection devices for flow cytometry fluorescence detection and chemiluminescence detection will lead to higher costs. In summary, the reliability of existing sample detection devices is poor. Summary of the Invention

[0004] The main technical problem to be solved by this application is how to improve the reliability of the sample detection device.

[0005] To solve the above technical problem, the first technical solution adopted by this application is: A sample detection device, comprising: a detection module, which is used to process a sample for flow cytometry fluorescence detection to make the sample emit a first light beam, or to process a sample for chemiluminescence detection to make the sample emit a second light beam; a sensing module, which is used to receive the first light beam or the second light beam and generate a corresponding electrical signal; a first circuit, which is used to convert the received electrical signal into an analog signal; a second circuit, which is used to convert the received electrical signal into a digital signal; a switching module, connected to the sensing module; a control module, which is used to: when the detection mode of the sample is flow cytometry fluorescence detection, control the switching module to connect to the first circuit and process the sample based on the analog signal to obtain the detection result of flow cytometry fluorescence detection; when the detection mode of the sample is chemiluminescence detection, control the switching module to connect to the second circuit and process the sample based on the digital signal to obtain the detection result of chemiluminescence detection.

[0006] Among them, the detection module includes: a flow cell; a chemiluminescence detection position; a sample delivery module, which is used to deliver the sample to the flow cell or deliver the sample-containing solution to the chemiluminescence detection position; the control module is further used for: when the detection mode of the sample is flow cytometry fluorescence detection, controlling the sample delivery module to deliver the sample to the flow cell, and controlling the light source to emit a detection beam to the flow cell, so that the sample flowing through the flow cell generates a first beam; when the detection mode of the sample is chemiluminescence detection, controlling the sample delivery module to deliver the sample to the chemiluminescence detection position, so that the sample emits a second beam at the chemiluminescence detection position.

[0007] Among them, controlling the sample delivery module to deliver the sample to the flow cell includes: controlling the sample delivery module to collect the sample from the reaction container containing the sample, and delivering the collected sample to the flow cell through the corresponding liquid path to generate a first beam in the flow cell; controlling the sample delivery module to deliver the sample to the chemiluminescence detection position includes: controlling the sample delivery module to move the reaction container containing the sample to the chemiluminescence detection position to generate a second beam at the chemiluminescence detection position.

[0008] Among them, the optical path for the first beam to be emitted to the sensing module at least partially overlaps with the optical path for the second beam to be emitted to the sensing module.

[0009] Among them, the chemiluminescence detection position is located on the optical path of the first beam; or, a semi-reflective and semi-transmissive lens is provided on the optical path of the first beam, and the semi-reflective and semi-transmissive lens is used for: when the detection mode of the sample is flow cytometry fluorescence detection, transmitting the first beam so that the sensing module receives the first beam; when the detection mode of the sample is chemiluminescence detection, reflecting the second beam so that the sensing module receives the second beam.

[0010] Among them, the sample detection device further includes a magnetic separation module; the magnetic separation module includes at least one preset position combination; the preset position combination is sequentially provided with a liquid injection position, at least one adsorption position and a liquid discharge position at intervals; when the detection mode of the sample is flow cytometry fluorescence detection, the first combined number of continuously arranged preset position combinations is the positions that the sample needs to pass through during a single magnetic separation and cleaning; when the detection mode of the sample is chemiluminescence detection, the second combined number of continuously arranged preset position combinations is the positions that the sample needs to pass through during a single magnetic separation and cleaning; the first combined number is a preset multiple of the second combined number; the preset multiple is an integer multiple greater than 1.

[0011] Among them, the magnetic separation module includes: a rotating member; and a motor for controlling the synchronous rotation of the rotating member around a rotating shaft. On the rotating member, at least two preset position combinations are sequentially arranged at intervals around the rotating shaft along the rotation direction of the rotating member. Among all the injection liquid levels, there are a common injection liquid level and a chemiluminescence injection liquid level, and there is at least one chemiluminescence injection liquid level between adjacent common injection liquid levels. Among all the discharge liquid levels, there are a common discharge liquid level and a chemiluminescence discharge liquid level, and there is at least one chemiluminescence discharge liquid level between adjacent common discharge liquid levels. Along the rotation direction of the rotating member, the total number of work positions from the common injection liquid level to the closest common discharge liquid level is a preset multiple of the total number of work positions from the common injection liquid level to the closest chemiluminescence discharge liquid level, and / or, along the rotation direction of the rotating member, the total number of work positions from the common injection liquid level to the closest common discharge liquid level is a preset multiple of the total number of work positions from the chemiluminescence injection liquid level to the closest common discharge liquid level. The total number of work positions is the total of all the injection liquid levels, all the adsorption positions, and all the discharge liquid levels within the corresponding range.

[0012] Among them, magnetic substances are provided on the injection liquid level, the discharge liquid level, and the adsorption position. When the detection mode of the sample is flow cytometry fluorescence detection, the sample is adsorbed when passing through the chemiluminescence injection liquid level and the chemiluminescence discharge liquid level.

[0013] Among them, the control module is further configured to: when the detection mode of the sample is flow cytometry fluorescence detection, control the motor to move the reaction vessel to the common injection liquid level for liquid injection, and then control the motor to move the reaction vessel along the rotation direction to the closest common discharge liquid level for liquid discharge; when the detection mode of the sample is chemiluminescence detection, control the motor to move the reaction vessel to the common injection liquid level for liquid injection, and then control the motor to move the reaction vessel along the rotation direction to the closest chemiluminescence discharge liquid level for liquid discharge, and / or, control the motor to move the reaction vessel to the chemiluminescence injection liquid level for liquid injection, and then control the motor to move the reaction vessel along the rotation direction to the closest chemiluminescence discharge liquid level for liquid discharge, and / or, control the motor to move the reaction vessel to the chemiluminescence injection liquid level for liquid injection, and then control the motor to move the reaction vessel along the rotation direction to the closest common discharge liquid level for liquid discharge.

[0014] To solve the above technical problems, the second technical solution adopted in this application is: a sample detection method, including: obtaining the detection mode corresponding to the sample; the detection mode includes at least one of flow cytometry fluorescence detection and chemiluminescence detection; in response to the detection mode being flow cytometry fluorescence detection, controlling the sensing module to be connected to the first circuit, and obtaining the detection result of the sample based on the analog signal output by the first circuit; in response to the detection mode being chemiluminescence detection, controlling the sensing module to be connected to the second circuit, and obtaining the detection result of the sample based on the digital signal output by the second circuit.

[0015] The beneficial effects of the present application are as follows: Different from the prior art, in the technical solution of the present application, the sample detection device simultaneously has a device that can perform flow cytometry fluorescence detection on the sample to emit a first light beam, and a device that can perform chemiluminescence detection on the sample to emit a second light beam. So that the sample detection device can not only, when the sensing module receives the first light beam, control the switching module to connect the first circuit and the sensing module, and convert the electrical signal output by the sensing module into an analog signal based on the first circuit to perform signal analysis and processing of flow cytometry fluorescence detection to obtain the detection result of flow cytometry fluorescence detection, but also, when the sensing module receives the second light beam, control the switching module to connect the second circuit and the sensing module, and convert the electrical signal output by the sensing module into a digital signal based on the second circuit to perform signal analysis and processing of chemiluminescence detection to obtain the detection result of chemiluminescence detection. Based on the above method, the sample detection device of the present application can perform flow cytometry fluorescence detection or chemiluminescence detection when the user's needs are different, that is, it has the ability to perform both flow cytometry fluorescence detection and chemiluminescence detection at the same time, making the functions of the sample detection device diversified, avoiding the situation where more than two sample detection devices are required to separately perform flow cytometry fluorescence detection and chemiluminescence detection, reducing costs, and improving the reliability of the sample detection device. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0017] Figure 1 is a schematic structural diagram of an embodiment of the sample detection device of the present application;

[0018] Figure 2 is one of the schematic structural diagrams of an embodiment of the detection module of the present application;

[0019] Figure 3 is the second of the schematic structural diagrams of an embodiment of the detection module of the present application;

[0020] Figure 4 is a schematic structural diagram of an embodiment of the sample detection device of the present application;

[0021] Figure 5 is a schematic structural diagram of an embodiment of the magnetic separation module of the present application;

[0022] Figure 6 is a schematic flowchart of an embodiment of the sample detection method of the present application.

[0023] Reference numerals: detection module 11, flow cell 111, chemiluminescence detection position 112, sensing module 12, first circuit 13, second circuit 14, switching module 15, control module 16, magnetic separation module 21, rotating member 211, reagent module 22, sample addition module 23, and incubation module 24. Detailed implementation manners

[0024] The present application will be further described in detail below with reference to the accompanying drawings and embodiments. It should be specifically noted that the following embodiments are only used to illustrate the present application, but do not limit the scope of the present application. Similarly, the following embodiments are only partial embodiments of the present application rather than all embodiments. All other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present application.

[0025] The mention of "embodiment" in this document means that a specific feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the present application. The phrase appears in various positions in the specification and does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment mutually exclusive with other embodiments. Those skilled in the art will explicitly and implicitly understand that the embodiments described herein can be combined with other embodiments.

[0026] In the description of the present application, it should be noted that unless otherwise clearly specified and limited, the terms "installed", "set", "connected", and "coupled" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium. For those of ordinary skill in the art, the specific meanings of the above terms in the present application can be understood according to specific situations.

[0027] The present application provides a sample detection device. Refer to Figure 1 , Figure 1 which is a schematic structural diagram of an embodiment of the sample detection device of the present application. As Figure 1 shown, the sample detection device includes a detection module 11, a sensing module 12, a first circuit 13, a second circuit 14, a switching module 15, and a control module 16.

[0028] The detection module 11 is used to process the sample by flow cytometry fluorescence detection to make the sample emit a first light beam, or to process the sample by chemiluminescence detection to make the sample emit a second light beam. Specifically, the detection module 11 may include an optical path system for flow cytometry fluorescence detection, such as a flow cell. The sample can be input into the flow cell and irradiated with a corresponding light source to excite the sample to generate fluorescence and emit the first light beam. The detection module 11 may also specifically include a position for the sample to perform chemiluminescence, such as a chemiluminescence detection position. The sample located at the chemiluminescence detection position can perform chemiluminescence and emit the second light beam when mixed with a corresponding reagent.

[0029] The sensing module 12 is used to receive the first light beam or the second light beam and generate a corresponding electrical signal. Specifically, the sensing module 12 performs photoelectric conversion on the first light beam or the second light beam emitted by the detection module 11 to generate a corresponding electrical signal for subsequent signal processing. The sensing module 12 may specifically be a photomultiplier tube (PMT), or other types of devices with photoelectric conversion capabilities, which are not limited herein. The sensing module 12 includes a photosensitive area for receiving the first light beam or the second light beam, and the received first light beam or second light beam is converted into a corresponding electrical signal by the sensing module 12.

[0030] Optionally, the optical path of the first light beam emitted to the sensing module and the optical path of the second light beam emitted to the sensing module may form an angle in the photosensitive area; in some embodiments, the optical path of the first light beam emitted to the sensing module and the optical path of the second light beam emitted to the sensing module may also at least partially overlap.

[0031] The first circuit 13 is used to convert the received electrical signal into an analog signal. Specifically, the first circuit 13 can be used to convert the electrical signal output by the sensing module 12 after photoelectric conversion into an analog signal. It should be noted that when performing flow cytometry fluorescence detection, the electrical signal is usually converted into an analog signal for subsequent processing and analysis to ensure that the accuracy of the corresponding detection result is high enough.

[0032] The second circuit 14 is used to convert the received electrical signal into a digital signal. Specifically, the second circuit 14 can be used to convert the electrical signal output by the sensing module 12 after photoelectric conversion into a digital signal. It should be noted that when performing chemiluminescence detection, the electrical signal is usually converted into a digital signal for subsequent processing and analysis to ensure that the accuracy of the corresponding detection result is high enough.

[0033] The switching module 15 is connected to the sensing module 12. The switching module 15 may have a common terminal, a first terminal, and a second terminal. The switching module 15 can be used to control its common terminal to connect to its first terminal, or control its common terminal to connect to its second terminal, realizing the function of switching between these two connections. The common terminal of the switching module 15 is connected to the output terminal of the sensing module 12 that outputs the electrical signal obtained by photoelectric conversion.

[0034] The control module 16 is used for:

[0035] When the detection mode of the sample is flow cytometry fluorescence detection, control the switching module 15 to connect to the first circuit 13, process the sample based on the analog signal, and obtain the detection result of the flow cytometry fluorescence detection.

[0036] When the detection mode of the sample is chemiluminescence detection, control the switching module 15 to connect to the second circuit 14, process the sample based on the digital signal, and obtain the detection result of the chemiluminescence detection.

[0037] Among them, the first terminal of the switching module 15 can be connected to the first circuit 13, the second terminal of the switching module 15 can be connected to the second circuit 14. By switching the connection relationship between its common terminal, first terminal, and second terminal, the switching module 15 can realize the switching of inputting the electrical signal into the first circuit 13 or the second circuit 14 for signal processing.

[0038] When a sample is to be detected, it can be judged whether the detection mode of the currently to-be-detected sample is flow cytometry fluorescence detection or chemiluminescence detection.

[0039] If the detection mode is flow cytometry fluorescence detection, the sample can be transported to the sampling position of the flow chamber for the flow chamber to sample and optically process the sample, excite the sample to output the first light beam. Moreover, control the switching module 15 to connect the sensing module 12 and the first circuit 13, so that the electrical signal can be processed by the first circuit 13 to output the corresponding analog signal, and then the analog signal can be processed based on the flow cytometry fluorescence algorithm to obtain the detection result.

[0040] If the detection mode is chemiluminescence detection, the sample that has undergone chemiluminescence treatment can be transported to the chemiluminescence detection position for the sample to perform chemiluminescence at this chemiluminescence detection position and output the second light beam. Moreover, control the switching module 15 to connect the sensing module 12 and the second circuit 14, so that the electrical signal can be processed by the second circuit 14 to output the corresponding digital signal, and then the digital signal can be processed based on the chemiluminescence algorithm to obtain the detection result.

[0041] Based on the above method, by connecting the switching module 15 to the output end of the same sensing module 12, and connecting the first circuit 13 for converting the analog signal and the second circuit 14 for converting the digital signal after the switching module 15, it is possible to switch to the most suitable branch respectively when performing flow cytometry fluorescence detection and chemiluminescence detection, thereby improving the accuracy of the detection results in different types of detection modes obtained by subsequent signal processing based on the electrical signals output by the sensing module 12. That is, the sample detection device can simultaneously have the ability to perform flow cytometry fluorescence detection and chemiluminescence detection, making the functions of the sample detection device diversified, avoiding the situation where two or more sample detection devices or photoelectric receivers corresponding to flow cytometry fluorescence detection and chemiluminescence detection respectively are required to perform flow cytometry fluorescence detection and chemiluminescence detection separately, reducing the cost, and improving the reliability of the sample detection device.

[0042] Specifically, the detection result may refer to the concentration of the sample, or other types of sample detection results, which are not limited here.

[0043] Different from the prior art, in the technical solution of this application, the sample detection device simultaneously has devices capable of performing flow cytometry fluorescence detection on the sample to emit a first light beam, and devices capable of performing chemiluminescence detection on the sample to emit a second light beam. When the sensing module receives the first light beam, the sample detection device can control the switching module to connect the first circuit and the sensing module, and convert the electrical signal output by the sensing module into an analog signal based on the first circuit to perform signal analysis and processing of flow cytometry fluorescence detection to obtain the detection result of flow cytometry fluorescence detection. When the sensing module receives the second light beam, the sample detection device can also control the switching module to connect the second circuit and the sensing module, and convert the electrical signal output by the sensing module into a digital signal based on the second circuit to perform signal analysis and processing of chemiluminescence detection to obtain the detection result of chemiluminescence detection. Based on the above method, the sample detection device of this application can perform flow cytometry fluorescence detection or chemiluminescence detection when the user's needs are different. That is, it can simultaneously have the ability to perform flow cytometry fluorescence detection and chemiluminescence detection, making the functions of the sample detection device diversified, avoiding the situation where two or more sample detection devices are required to perform flow cytometry fluorescence detection and chemiluminescence detection separately, reducing the cost, and improving the reliability of the sample detection device.

[0044] In one embodiment, the first circuit 13 includes a DC amplifier, a low-pass filter, and a digital-to-analog converter.

[0045] The input end of the DC amplifier is connected to the switching module 15, the input end of the low-pass filter is connected to the output end of the DC amplifier, the input end of the digital-to-analog converter is connected to the output end of the low-pass filter, and the input end of the digital-to-analog converter is used to output the above analog signal.

[0046] The second circuit 14 includes a pulse amplifier, a discriminator, a pulse shaper and a pulse counter.

[0047] The input end of the pulse amplifier is connected to the switching module 15, the input end of the discriminator is connected to the output end of the pulse amplifier, the input end of the pulse shaper is connected to the output end of the discriminator, the input end of the pulse counter is connected to the output end of the pulse shaper, and the output end of the pulse counter is used to output the above-mentioned digital signal.

[0048] The analog signal or digital signal may be received by a computer or other device with processing capability to perform corresponding processing to obtain the detection result of flow fluorescence detection or the detection result of chemiluminescence detection.

[0049] Based on the above method, the sample detection device can simultaneously have the ability to output the analog signal required for flow fluorescence detection, or the digital signal required for chemiluminescence detection, and under different user requirements, convert and output voltage signals with the characteristics required for the corresponding detection to obtain detection results, thereby improving the flexibility of the sample detection device and further improving the reliability of the sample detection device.

[0050] In one embodiment, see Figure 2 and Figure 3 , Figure 2 is one of the structural diagrams of an embodiment of the detection module of the present application, Figure 3 This is a second structural diagram of an embodiment of the detection module of the present application. Figure 2 and Figure 3 As shown, the detection module 11 includes a flow chamber 111, a chemiluminescent detection site 112 and a sample delivery module (not shown), and may also include light sources A1 and A2, and the sensor module 12 may include sensors B1 and B2.

[0051] The sample transport module is used to transport the sample to the flow chamber 111 or to transport the sample to the chemiluminescence detection position 112 .

[0052] The control module 16 is also used for:

[0053] When the detection mode of the sample is flow fluorescence detection, the sample delivery module is controlled to deliver the sample to the flow chamber 111 , and the light source is controlled to emit a detection beam to the flow chamber 111 , so that the sample flowing through the flow chamber 111 generates a first beam.

[0054] When the detection mode of the sample is chemiluminescence detection, the sample delivery module is controlled to deliver the sample to the chemiluminescence detection position 112 , so that the sample emits a second light beam at the chemiluminescence detection position 112 .

[0055] Specifically, Figure 2and 3 As shown, the step of controlling the sample delivery module to deliver the sample to the flow chamber 111 may specifically include: controlling the sample delivery module to collect the sample from the reaction container containing the sample, and delivering the collected sample to the flow chamber 111 through the corresponding liquid path to generate a first light beam in the flow chamber 111.

[0056] The sample transport module may include a gripper and a liquid path unit of a flow chamber. When flow fluorescence detection is required, the corresponding gripper can be controlled to move the reaction container containing the sample to the sampling position of the flow chamber 111, and the liquid path unit of the flow chamber can be controlled to collect the sample from the reaction container at the sampling position to transport it to the flow chamber 111 for corresponding processing and emit a first light beam.

[0057] The step of controlling the sample transport module to transport the sample to the chemiluminescent detection position 112 may specifically include: controlling the sample transport module to move the reaction container containing the sample to the chemiluminescent detection position 112 to generate a second light beam at the chemiluminescent detection position 112 .

[0058] The sample transport module may include a gripper. When chemiluminescence detection is required, the corresponding gripper can be controlled to move the reaction container containing the sample to the chemiluminescence detection position 112. The reaction container containing the sample that has been treated with chemiluminescence and added with corresponding reagents can emit a second light beam through direct luminescence, indirect luminescence or other chemiluminescence methods.

[0059] Optionally, in some embodiments, the chemiluminescence detection position 112 may also adopt a circulation pool setting, and the circulation pool may share the liquid path unit of the flow chamber. When chemiluminescence detection is required, the corresponding gripper may be controlled to move the reaction container containing the sample to the sampling position of the flow chamber 111, and the liquid path unit of the flow chamber may be controlled to collect the sample from the reaction container at the sampling position to transport it to the circulation pool and emit a second light beam.

[0060] like Figure 2 and 3 As shown, in flow fluorescence detection, usually at least one light source is used, such as light source A1 and light source A2 that emit light beams of different wavelengths, to emit light beams to the sample in the flow chamber 111 to achieve sample detection of multiple detection items at the same time, thereby stimulating the generation of two first light beams corresponding to light source A1 and light source A2, respectively. Sensors B1 and sensor B2 are respectively used to receive the two first light beams. More light sources and corresponding sensors can also be provided, which is not limited here.

[0061] In chemiluminescence detection, since its principle is to make the sample chemiluminescent by adding corresponding reagents, usually only one light beam emitted by it is detected in one chemiluminescence detection, that is, the second light beam is received and detected. It should be noted that since flow fluorescence detection is not performed at this time, but chemiluminescence detection is performed, the second light beam can be received based on any sensor (such as sensor B1) used in flow fluorescence detection. Optionally, since the sensitivity and resolution of the received light in each light detection channel are different during flow fluorescence detection, the sensor performance corresponding to each light detection channel is different. The sample detection device provided in the embodiment of the present application can share the sensor with the best performance in flow fluorescence detection with chemiluminescence detection to obtain the most accurate detection results at low cost.

[0062] It should be noted that the flow fluorescence detection may include the detection of at least one classified fluorescent substance, that is, in a single flow fluorescence detection, only one classified fluorescent substance may be detected, or more than two classified fluorescent substances may be detected simultaneously.

[0063] In order to realize the detection of more than two classified fluorescent objects at the same time, a quantitative fluorescence receiver and at least one classified fluorescence receiver, as well as a quantitative light source and at least one classified light source can be set in the sample detection device. The quantitative light source can be used to emit a light beam to the sample to excite and generate a corresponding fluorescent beam, and the quantitative fluorescence receiver is used to receive the fluorescent beam corresponding to the quantitative light source. The classified light source can be used to emit a light beam to the sample to excite and generate a corresponding fluorescent beam, and the classified fluorescence receiver is used to receive the fluorescent beam corresponding to the corresponding classified light source, and then the electrical signals converted and generated by the quantitative fluorescence receiver and the at least one classified fluorescence receiver are processed to obtain the results of flow fluorescence detection of more than two classified fluorescent objects.

[0064] Optionally, more than two fluorescent light beams can be excited by classification light sources corresponding to more than two classification fluorescent objects, or more than two fluorescent light beams corresponding to different classification fluorescent objects can be excited by one classification light source to be received by more than two classification fluorescent receivers.

[0065] Optionally, the sensor with the best performance shared with chemiluminescence detection may be a receiver with the best performance among a quantitative fluorescence receiver and at least one classification fluorescence receiver, and the fluorescence light beam received by the sensor with the best performance is the first light beam.

[0066] Based on the above method, a sample detection device that can be used for flow fluorescence detection and chemiluminescence detection, respectively, can be constructed based on as few light sources and sensors as possible, thereby improving the applicability of the optical path of the sample detection device, thereby improving the flexibility of the sample detection device, and further improving the reliability of the sample detection device.

[0067] Optionally, the optical path of the first light beam emitted to the sensor module 12 at least partially overlaps with the optical path of the second light beam emitted to the sensor module 12 .

[0068] Specifically, Figure 2 and Figure 3 As shown, the overlapping optical path C is the partial optical path where the first light beam and the second light beam overlap before entering the sensor module 12. By making at least a portion of the optical paths of the first light beam and the second light beam overlap before entering the sensor module 12, it is possible to receive two light beams through the same sensor in the sensor module 12, that is, the same sensor can receive different light beams when performing flow fluorescence detection and chemiluminescence detection, respectively, to achieve multiplexing, thereby improving the utilization rate of the sensor module 12, reducing costs, and reducing the volume of the sample detection device, further improving the reliability of the sample detection device.

[0069] Furthermore, if Figure 3 As shown, the chemiluminescent detection position 112 is located on the optical path of the first light beam.

[0070] or,

[0071] like Figure 2 As shown, a half-reflecting half-mirror D is provided on the optical path of the first light beam, and the half-reflecting half-mirror D is used to transmit the first light beam when the detection mode of the sample is flow fluorescence detection so that the sensor module 12 receives the first light beam, and reflect the second light beam when the detection mode of the sample is chemiluminescence detection so that the sensor module 12 receives the second light beam.

[0072] Specifically, the chemiluminescence detection position 112 can be directly set in the optical path of the first light beam so that the first light beam generated by the flow fluorescence detection and the second light beam generated by the chemiluminescence detection can both enter the sensor of the same sensing module 12, realize photoelectric conversion to generate corresponding electrical signals, and obtain detection results.

[0073] It is also possible to set a half-reflective half-mirror D at the intersection of the direction of the second light beam emitted when the reaction container is at the chemiluminescence detection position 112 and the light path of the first light beam, so as to reflect the second light beam to the light path of the first light beam emitted to the sensor module 12, so that the first light beam generated by the flow fluorescence detection and the second light beam generated by the chemiluminescence detection can both enter the sensor of the same sensor module 12, realize photoelectric conversion to generate corresponding electrical signals, and obtain detection results.

[0074] Based on the above method, by directly or indirectly emitting the second light beam into the sensor module 12 into which the first light beam is emitted, it is possible to receive two light beams through the same sensor in the sensor module 12, that is, the same sensor can receive different light beams when performing flow fluorescence detection and chemiluminescence detection, respectively, to achieve multiplexing, thereby improving the utilization rate of the sensor module 12, reducing costs, and reducing the volume of the sample detection device, thereby further improving the reliability of the sample detection device.

[0075] In one embodiment, the sample detection device further includes a magnetic separation module.

[0076] The magnetic separation module comprises at least one preset position combination, wherein a liquid injection position, at least one adsorption position and a liquid discharge position are sequentially arranged at intervals.

[0077] When the detection mode of the sample is flow fluorescence detection, the preset position combinations of the first combination quantity that are set continuously are the positions that the sample needs to pass through in a single magnetic separation and washing.

[0078] When the detection mode of the sample is chemiluminescence detection, the preset position combinations of the second combination quantity that are set continuously are the positions that the sample needs to pass through in a single magnetic separation and cleaning.

[0079] The first combination quantity is a preset multiple of the second combination quantity. The preset multiple is an integer multiple greater than 1.

[0080] Specifically, see Figure 4 and Figure 5 , Figure 4 is a schematic structural diagram of an embodiment of a sample detection device of the present application, Figure 5 It is a structural schematic diagram of an embodiment of the magnetic separation module of the present application.

[0081] First, as Figure 4 As shown, the sample detection device may include a magnetic separation module 21 , a reagent module 22 , a sample adding module 23 and an incubation module 24 .

[0082] The first gripper may be controlled to pass through the first track L1 to move the reaction container containing the sample onto the sample loading module 23 .

[0083] Control the sample loading module 23 to rotate so that the reaction container rotates into the moving range of the second gripper, control the second gripper to pass through the second track L2, move the reaction container from the sample loading module 23 to the reagent module 22 for adding reagents for flow fluorescence detection or chemiluminescence detection or other types of detection, and then control the second gripper to pass through the second track L2 to move the reaction container back to the sample loading module 23.

[0084] The reagent module 22 has reagent kits required for flow fluorescence detection or chemiluminescence detection or other types of detection, which can be moved to the moving range of the second gripper by rotating the reagent module 22 to add corresponding reagents.

[0085] Control the sample loading module 23 to rotate so that the reaction container rotates within the moving range of the third gripper, control the third gripper to pass through the third trajectory L3, move the reaction container from the sample loading module 23 to the incubation module 24 for flow fluorescence detection or chemiluminescence detection or other types of incubation operations, and control the third gripper to pass through the third trajectory L3 to move the reaction container from the incubation module 24 back to the sample loading module 23.

[0086] The third track L3 passes through the magnetic separation module 21, the sample addition module 23, the incubation module 24, Figure 4 The flow chamber sample position M and Figure 4 The chemiluminescent detection position N is shown.

[0087] The third gripper is controlled to pass through the third trajectory L3, and the reaction container is moved from the sample loading module 23 to the magnetic separation module 21 for magnetic separation and cleaning. Subsequently, it can be determined whether the reaction container still needs to undergo the next round of magnetic separation and cleaning. If necessary, the corresponding gripper can be controlled to move the reaction container back to the sample loading module 23, and execute the above-mentioned steps of processing through the reagent module 22, the incubation module 24, and the magnetic separation module 21. If not necessary, the third gripper can be controlled to place the reaction container after magnetic separation and cleaning at the sampling position M of the flow chamber for flow fluorescence detection processing, emitting a first light beam, or to place it at the chemiluminescence detection position N for chemiluminescence detection processing, emitting a second light beam.

[0088] Second, if Figure 5 As shown, the magnetic separation device may include at least one preset position combination, and a single preset position combination may be provided with a filling position, at least one adsorption position and a liquid discharge position in sequence. For example, the filling position A1-at least one adsorption position B-liquid discharge position C1 is a preset position combination, and so on, the filling position A2-at least one adsorption position B-liquid discharge position C2, the filling position A3-at least one adsorption position B-liquid discharge position C3, and the filling position A4-at least one adsorption position B-liquid discharge position C4 are respectively a preset position combination.

[0089] It should be noted that in the magnetic separation and cleaning of flow fluorescence detection, due to the slow response of the magnetic balls, a longer magnetic adsorption time is required before draining. Otherwise, the magnetic balls are easily drained during drainage because they are not completely adsorbed, resulting in serious loss of magnetic balls and abnormal results. However, due to its low sensitivity, the number of magnetic separation and cleaning required is relatively small. In the magnetic separation and cleaning of chemiluminescence detection, due to the fast response of the magnetic balls, a shorter adsorption time is required to complete the adsorption. However, due to its high sensitivity, more magnetic separation and cleaning are required.

[0090] Therefore, by making the number of first combinations a preset multiple of the number of second combinations, and the preset multiple being an integer multiple greater than 1, when the detection mode is flow fluorescence detection, the sample passes through more adsorption sites for adsorption in a single magnetic separation cleaning, and because a larger number of first combinations are occupied, the number of cleaning times after magnetic separation cleaning is performed on all stations on the magnetic separation module is less; and, when the detection mode is chemiluminescence detection, the sample passes through fewer adsorption sites for adsorption in a single magnetic separation cleaning, and because a larger number of second combinations are occupied, the number of cleaning times after magnetic separation cleaning is performed on all stations on the magnetic separation module is more, thereby achieving the technical effect of providing a corresponding magnetic separation detection method according to the requirements of different detection modes in the same magnetic separation module 21, that is, avoiding the situation where a separate magnetic separation module 21 must be configured for the two detection modes respectively, thereby improving the utilization rate of the magnetic separation module 21, reducing the total volume of the sample detection device where the magnetic separation module 21 is located, reducing the cost of the magnetic separation module 21, and improving the reliability of the sample detection device.

[0091] Alternatively, if Figure 5 As shown, the magnetic separation module 21 includes a rotating member 211 and a motor (not shown), and the motor is used to control the rotating member 211 to rotate synchronously around the rotating shaft.

[0092] Wherein, on the rotating member 211 , at least two preset position combinations are sequentially arranged at intervals around the rotating axis along the rotating direction D of the rotating member 211 .

[0093] All the filling positions include common filling positions and chemiluminescent filling positions, and there is at least one chemiluminescent filling position between adjacent common filling positions. All the drainage positions include common drainage positions and chemiluminescent drainage positions, and there is at least one chemiluminescent drainage position between adjacent common drainage positions.

[0094] Along the rotation direction D of the rotating member 211, the total number of workstations from the common filling position to the common discharge position closest thereto is a preset multiple of the total number of workstations from the common filling position to the chemiluminescent discharge position closest thereto, and / or, along the rotation direction D of the rotating member 211, the total number of workstations from the common filling position to the common discharge position closest thereto is a preset multiple of the total number of workstations from the chemiluminescent filling position to the common discharge position closest thereto.

[0095] Among them, all the injection positions, all the adsorption positions and all the drainage positions are the workstations on the rotating member 211, and the total number of workstations is the total number of all the injection positions, all the adsorption positions and all the drainage positions within the corresponding range.

[0096] Specifically, for example, Figure 5 As shown, the filling position A1 and the filling position A3 can be the above-mentioned common filling position, the filling position A2 and the filling position A4 can be the above-mentioned chemiluminescent filling positions, the discharge position C2 and the discharge position C4 can be the above-mentioned common filling position, and the discharge position C1 and the discharge position C3 can be the above-mentioned chemiluminescent filling positions.

[0097] The total number of workstations from the common liquid filling position to the common liquid discharge position closest thereto may be, for example, 8 workstations from the liquid filling position A1 (common liquid filling position) to the liquid discharge position C2 (common liquid discharge position).

[0098] The total number of workstations from the common liquid filling position to the closest chemiluminescent liquid discharge position may be, for example, 4 workstations from the liquid filling position A1 (common liquid filling position) to the liquid discharge position C1 (chemiluminescent liquid discharge position).

[0099] The total number of workstations from the chemiluminescent filling position to the closest shared liquid discharge position may be, for example, 4 workstations from the filling position A2 (chemiluminescent filling position) to the liquid discharge position C2 (shared liquid discharge position).

[0100] It can be seen that in this example, the preset multiple is 2.

[0101] When the detection mode is flow fluorescence detection, the reaction container can be filled at the filling position A1, drained at the draining position C2, filled at the filling position A3, and drained at the draining position C4, that is, all fillings and all drains are carried out at the common filling position and the common draining position, so that in the flow fluorescence detection, the number of stations corresponding to a single magnetic separation cleaning is larger, so the time is longer, and the total number of magnetic separation cleanings is smaller.

[0102] When the detection mode is chemiluminescence detection, the reaction container can be filled at the filling position A1, drained at the draining position C1, filled at the filling position A2, drained at the draining position C2, filled at the filling position A3, drained at the draining position C3, filled at the filling position A4, and drained at the draining position C4, that is, filling and draining are performed at the common filling position and the common draining position, and at the chemiluminescence filling position and the chemiluminescence draining position, so that in the chemiluminescence detection, the number of stations that need to be passed through for a single magnetic separation cleaning is smaller, so that the time is shorter, and the total number of magnetic separation cleanings is greater.

[0103] The above is only an example in which there is a chemiluminescent filling position between adjacent shared filling positions, and there is a chemiluminescent drainage position between adjacent shared drainage positions, and the preset multiple is 2. There may also be more than two chemiluminescent filling positions or chemiluminescent drainage positions, so that the preset multiple is greater than 2, which will not be repeated here.

[0104] Based on the above method, the sample detection device can perform magnetic separation and cleaning operations with different single magnetic separation and cleaning durations and different total magnetic separation and cleaning times when performing flow fluorescence detection and chemiluminescence detection, respectively, thereby improving the adaptability of the sample detection device in dealing with different detection modes, and further improving the accuracy of the detection results when performing flow fluorescence detection and chemiluminescence detection.

[0105] Furthermore, magnetic materials are arranged on the liquid filling position, liquid discharge position and adsorption position.

[0106] When the detection mode of the sample is flow fluorescence detection, the sample is adsorbed when passing through the chemiluminescence injection position and the chemiluminescence discharge position.

[0107] Specifically, magnetic materials may be arranged at all workstations of the rotating member 211 . The magnetic materials may be permanent magnets or other types of materials with magnetic adsorption capability, which are not limited here.

[0108] The distance between the magnetic material and its corresponding workstation is not less than 0.5 mm and not more than 5 mm.

[0109] Based on the above method, it can be ensured that the magnetic material has sufficient magnetic adsorption capacity, but it will not cause unexpected situations in magnetic separation and cleaning due to excessive magnetic adsorption capacity. That is, the magnetic material can have a more suitable magnetic adsorption capacity relative to its corresponding filling position, discharge position or adsorption position, thereby improving the reliability of the sample detection device.

[0110] Furthermore, a substrate bottom liquid level F and a cup transfer position E may be provided on the rotating member 211 .

[0111] Specifically, when the reaction container is moved onto the rotating member 211, the corresponding gripper can be controlled to move the reaction container to the cup transfer position E. When the reaction container is moved out of the rotating member 211, the reaction container can be moved to the cup transfer position E for the corresponding gripper to grab and move away.

[0112] In the process of magnetic separation and cleaning, taking chemiluminescence detection as an example, the reaction container can be moved to the cup transfer position, and the rotating member 211 can be controlled to rotate so that the reaction container passes through multiple preset position combinations in sequence to complete multiple magnetic separation and cleaning. After the cleaning is completed, it is determined whether there is still a magnetic separation and cleaning step in the subsequent steps. If not, the reaction container is moved to the substrate bottom liquid level, the substrate is added, and then the reaction container is moved to the cup transfer position for the gripper to grab and move away for subsequent related operations. If it exists, the reaction container is moved to the cup transfer position for the gripper to grab and move away for subsequent related operations.

[0113] Taking flow fluorescence detection as an example, the reaction container can be moved to the cup transfer position, and the rotating member 211 can be controlled to rotate so that the reaction container passes through multiple preset position combinations in sequence to complete multiple magnetic separation cleanings, and after the cleaning is completed, it is determined whether there is still a magnetic separation cleaning step in the subsequent steps. If not, the reaction container is moved to the substrate bottom liquid level, the bottom liquid is added, and then the reaction container is moved to the cup transfer position for the gripper to grab and move away for subsequent related operations. If it exists, the reaction container is moved to the cup transfer position for the gripper to grab and move away for subsequent related operations.

[0114] The sample detection device may further include a liquid delivery module.

[0115] First, the liquid delivery module includes a waste unit, 4 waste pumps and 4 discharge needles.

[0116] The waste liquid unit can be connected to one end of four waste liquid pumps through a liquid path, and the other end of the waste liquid pump is connected to a liquid discharge needle. The liquid discharge needle can be moved to 3-30 mm above the reaction container at the liquid discharge position, and then the waste liquid pump is driven to suck the supernatant in the reaction container into the waste liquid unit to complete the liquid discharge. The four liquid discharge needles can correspond to the liquid discharge positions C1-C4 described in the above embodiment.

[0117] Second, the liquid delivery module includes a first reagent unit, a first suction and discharge unit and a substrate needle.

[0118] The first reagent unit can be used to store a substrate for chemiluminescent detection. The first suction and discharge unit can be used to suck the substrate from the first reagent unit and discharge it to the substrate needle, and the substrate needle can be used to move to 3-30 mm above the reaction container at the substrate bottom liquid level F to discharge the substrate.

[0119] Third, the liquid delivery module includes a second reagent unit, a second suction and exhalation unit, 4 injection needles and a bottom liquid needle.

[0120] The second reagent unit can be used to store reagents used as a base liquid for flow fluorescence detection and as a cleaning liquid for magnetic separation cleaning. The second suction and discharge unit can be used to suck the substrate from the second reagent unit and discharge it to the injection needle and the base liquid needle. The base liquid needle can be used to extend into the reaction container located at the bottom liquid level F of the substrate to discharge the base liquid, and the injection needle can be used to move to 3-30 mm above the reaction container located at the injection position to discharge the cleaning liquid. The four injection needles can correspond to the injection positions A1-A4 described in the previous embodiment one by one.

[0121] Furthermore, the control module 16 is also used for:

[0122] When the detection mode of the sample is flow fluorescence detection, the motor is controlled to move the reaction container to the common filling position for filling, and then the motor is controlled to move the reaction container along the rotation direction to the closest common discharge position for discharge.

[0123] When the detection mode of the sample is chemiluminescence detection, the motor is controlled to move the reaction container to the common filling position for filling, and then the motor is controlled to move the reaction container along the rotation direction to the closest chemiluminescence discharge position for discharge.

[0124] And / or, control the motor to move the reaction container to the chemiluminescent filling position for filling, and then control the motor to move the reaction container along the rotation direction to the closest chemiluminescent discharge position for discharge,

[0125] And / or, the motor is controlled to move the reaction container to the chemiluminescent liquid filling position for liquid filling, and then the motor is controlled to move the reaction container along the rotation direction to the closest common liquid discharge position for liquid discharge.

[0126] Specifically, when the detection mode of the sample is flow fluorescence detection, the rotating member 211 can be controlled to rotate one circle, and the reaction container is filled with liquid when passing through each common filling position, and is drained when passing through each common drain position, so as to achieve magnetic separation and cleaning. For example, a magnetic separation and cleaning is completed by filling liquid at the filling position A1 and draining liquid at the draining position C2, and a magnetic separation and cleaning is completed by filling liquid at the filling position A3 and draining liquid at the draining position C4, for a total of 2 magnetic separation and cleaning, and each magnetic separation and cleaning needs to pass through 8 working positions.

[0127] When the detection mode of the sample is flow cytometry fluorescence detection, the rotating member 211 can be controlled to rotate one circle, and the reaction container is filled with liquid when passing through each filling level and drained when passing through each drainage level, so as to realize magnetic separation cleaning. For example, filling the liquid at the filling level A1 and draining the liquid at the drainage level C1 to complete a magnetic separation cleaning; filling the liquid at the filling level A2 and draining the liquid at the drainage level C2 to complete a magnetic separation cleaning; filling the liquid at the filling level A3 and draining the liquid at the drainage level C3 to complete a magnetic separation cleaning; filling the liquid at the filling level A4 and draining the liquid at the drainage level C4 to complete a magnetic separation cleaning. There are a total of 4 magnetic separation cleanings, and each magnetic separation cleaning requires the duration of 4 workstations.

[0128] Based on the above method, the same rotating member 211 can be used to plan different filling and draining workstations when adapting to flow cytometry fluorescence detection and chemiluminescence detection respectively. When the rotating member 211 rotates one week, it can provide fewer magnetic separation cleanings with longer single - time duration for flow cytometry fluorescence detection and more magnetic separation cleanings with shorter single - time duration for chemiluminescence detection, so as to adapt to the requirements of different detection modes and improve the accuracy of detection results when performing flow cytometry fluorescence detection and chemiluminescence detection.

[0129] This application also proposes a sample detection method. Refer to Figure 6 , Figure 6 is a schematic flowchart of an embodiment of the sample detection method of this application. As Figure 6 shown, the sample detection method includes:

[0130] Step S11: Obtain the detection mode corresponding to the sample. Among them, the detection mode includes at least one of flow cytometry fluorescence detection and chemiluminescence detection.

[0131] Step S12: In response to the detection mode being flow cytometry fluorescence detection, control the sensing module 12 to be connected to the first circuit 13, and obtain the detection result of the sample based on the analog signal output by the first circuit 13.

[0132] Step S13: In response to the detection mode being chemiluminescence detection, control the sensing module 12 to be connected to the second circuit 14, and obtain the detection result of the sample based on the digital signal output by the second circuit 14. Specifically, the sample detection method may also include the steps performed by the control module 16 described in any of the previous embodiments, which will not be elaborated here.

[0133] Different from the prior art, in the technical solution of the present application, the sample detection device is internally provided with a device capable of performing flow cytometry fluorescence detection on a sample to emit a first light beam, and a device capable of performing chemiluminescence detection on the sample to emit a second light beam. Such that the sample detection device can not only, when the sensing module receives the first light beam, control the switching module to connect the first circuit and the sensing module, and convert the electrical signal output by the sensing module into an analog signal based on the first circuit to perform signal analysis and processing of flow cytometry fluorescence detection to obtain the detection result of flow cytometry fluorescence detection, but also, when the sensing module receives the second light beam, control the switching module to connect the second circuit and the sensing module, and convert the electrical signal output by the sensing module into a digital signal based on the second circuit to perform signal analysis and processing of chemiluminescence detection to obtain the detection result of chemiluminescence detection. Based on the above method, the sample detection device of the present application can perform flow cytometry fluorescence detection or chemiluminescence detection when the user's requirements are different, that is, it has the ability to perform both flow cytometry fluorescence detection and chemiluminescence detection at the same time, making the functions of the sample detection device diversified, avoiding the situation where more than two sample detection devices are required to separately perform flow cytometry fluorescence detection and chemiluminescence detection, reducing costs, and improving the reliability of the sample detection device.

[0134] In the description of the present application, the descriptions with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples", etc. mean that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, without contradiction, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples.

[0135] In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include at least one of the features. In the description of the present application, the meaning of "a plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0136] Any process or method description represented in a flowchart or otherwise described herein can be understood to represent a module, segment, or portion of code including one or more executable instructions for implementing a specific logical function or process. The scope of the preferred embodiments of the present application includes additional implementations, where the functions may be performed in an order not shown or discussed, including in a substantially simultaneous manner or in a reverse order according to the functions involved, which should be understood by those skilled in the technical field to which the embodiments of the present application pertain.

[0137] The logic and / or steps represented in a flowchart or otherwise described herein, for example, can be considered a sequenced list of executable instructions for implementing a logical function, and can be specifically implemented in any computer-readable medium for use by or in connection with an instruction execution system, apparatus, or device (which can be a personal computer, server, network device, or other system that can fetch and execute instructions from the instruction execution system, apparatus, or device). For the purposes of this specification, a "computer-readable medium" can be any device that can contain, store, communicate, propagate, or transport a program for use by or in connection with an instruction execution system, apparatus, or device. More specific examples (a non-exhaustive list) of the computer-readable medium include the following: an electrical connection portion having one or more wirings (electronic device), a portable computer diskette (magnetic device), a random access memory (RAM), a read-only memory (ROM), an erasable programmable read-only memory (EPROM or flash memory), an optical fiber device, and a portable compact disc read-only memory (CDROM). Additionally, the computer-readable medium can even be paper or other suitable medium on which the program can be printed, as the program can be obtained electronically, for example, by optically scanning the paper or other medium, followed by editing, interpretation, or otherwise processing as appropriate, and then storing it in a computer memory.

[0138] The above are only the embodiments of the present application, and do not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made by using the content of the specification and drawings of the present application, or directly or indirectly applied in other related technical fields, shall be equally included in the patent protection scope of the present application.

Claims

1. A sample detection device, characterized in that, Comprising: A detection module, which is used to perform flow cytometry fluorescence detection processing on a sample so that the sample emits a first light beam, or is used to perform chemiluminescence detection processing on the sample so that the sample emits a second light beam; A sensing module, which is used to receive the first light beam or the second light beam and generate a corresponding electrical signal; A first circuit, which is used to convert the received electrical signal into an analog signal; A second circuit, which is used to convert the received electrical signal into a digital signal; A switching module, connected to the sensing module; A control module, the control module is used for: When the detection mode of the sample is flow cytometry fluorescence detection, controlling the switching module to connect to the first circuit and processing the sample based on the analog signal to obtain a detection result of flow cytometry fluorescence detection; When the detection mode of the sample is chemiluminescence detection, controlling the switching module to connect to the second circuit and processing the sample based on the digital signal to obtain a detection result of chemiluminescence detection.

2. The sample detection device according to claim 1, wherein The detection module includes: A flow cell; A chemiluminescence detection position; A sample delivery module, which is used to deliver the sample to the flow cell or deliver the reaction container containing the sample to the chemiluminescence detection position; The control module is further used for: When the detection mode of the sample is flow cytometry fluorescence detection, controlling the sample delivery module to deliver the sample to the flow cell and controlling a light source to emit a detection light beam to the flow cell so that the sample flowing through the flow cell generates the first light beam; When the detection mode of the sample is chemiluminescence detection, controlling the sample delivery module to deliver the sample to the chemiluminescence detection position so that the sample emits the second light beam at the chemiluminescence detection position.

3. The sample detection device according to claim 2, wherein Controlling the sample delivery module to deliver the sample to the flow cell includes: controlling the sample delivery module to collect the sample from a reaction container containing the sample and deliver the collected sample to the flow cell through a corresponding liquid path to generate the first light beam in the flow cell; Controlling the sample delivery module to deliver the sample to the chemiluminescence detection position includes: controlling the sample delivery module to move the reaction container containing the sample to the chemiluminescence detection position to generate the second light beam at the chemiluminescence detection position.

4. The sample detection device according to claim 2 or 3, characterized in that, The optical path of the first light beam emitted to the sensing module at least partially overlaps with the optical path of the second light beam emitted to the sensing module.

5. The sample detection device according to claim 4, wherein, The chemiluminescence detection position is located on the optical path of the first light beam; Or, A semi-reflective semi-transmissive lens is provided on the optical path of the first light beam, and the semi-reflective semi-transmissive lens is used for: when the detection mode of the sample is flow cytometry fluorescence detection, transmitting the first light beam so that the sensing module receives the first light beam; when the detection mode of the sample is chemiluminescence detection, reflecting the second light beam so that the sensing module receives the second light beam.

6. The sample detection device according to any one of claims 1, 2, 3, and 5, characterized in that, The sample detection device further includes a magnetic separation module; The magnetic separation module includes at least one preset position combination; in the preset position combination, a liquid injection position, at least one adsorption position, and a liquid discharge position are sequentially arranged at intervals. When the detection mode of the sample is flow cytometry fluorescence detection, the preset position combinations with the first combined quantity set continuously are the positions that the sample needs to pass through during a single magnetic separation and cleaning. When the detection mode of the sample is chemiluminescence detection, the preset position combinations with the second combined quantity set continuously are the positions that the sample needs to pass through during a single magnetic separation and cleaning. The first combined quantity is a preset multiple of the second combined quantity; the preset multiple is an integer multiple greater than 1.

7. The sample detection device according to claim 6, wherein The magnetic separation module includes: A rotating member; A motor for controlling the synchronous rotation of the rotating member around a rotating shaft. Wherein, on the rotating member, at least two of the preset position combinations are sequentially arranged at intervals around the rotating shaft along the rotation direction of the rotating member. Among all the liquid injection positions, there are a common liquid injection position and a chemiluminescence liquid injection position, and there is at least one chemiluminescence liquid injection position between adjacent common liquid injection positions; among all the liquid discharge positions, there are a common liquid discharge position and a chemiluminescence liquid discharge position, and there is at least one chemiluminescence liquid discharge position between adjacent common liquid discharge positions. Along the rotation direction of the rotating member, the total number of work positions from the common liquid injection position to the closest common liquid discharge position is the preset multiple of the total number of work positions from the common liquid injection position to the closest chemiluminescence liquid discharge position, and / or, along the rotation direction of the rotating member, the total number of work positions from the common liquid injection position to the closest common liquid discharge position is the preset multiple of the total number of work positions from the chemiluminescence liquid injection position to the closest common liquid discharge position; Wherein, the total number of work positions is the total number of all the liquid injection positions, all the adsorption positions, and all the liquid discharge positions within the corresponding range.

8. The sample detection device according to claim 7, wherein Magnetic substances are provided at the liquid injection position, the liquid discharge position, and the adsorption position. When the detection mode of the sample is flow cytometry fluorescence detection, the sample is adsorbed when passing through the chemiluminescence liquid injection position and the chemiluminescence liquid discharge position.

9. The sample detection device according to claim 7 or 8, characterized in that, The control module is further configured to: When the detection mode of the sample is flow cytometry fluorescence detection, control the motor to move the reaction container to the common liquid injection position for liquid injection, and then control the motor to move the reaction container along the rotation direction to the closest common liquid discharge position for liquid discharge; When the detection mode of the sample is chemiluminescence detection, control the motor to move the reaction container to the common liquid injection position for liquid injection, and then control the motor to move the reaction container along the rotation direction to the closest chemiluminescence liquid discharge position for liquid discharge, and / or, control the motor to move the reaction container to the chemiluminescence liquid injection position for liquid injection, and then control the motor to move the reaction container along the rotation direction to the closest chemiluminescence liquid discharge position for liquid discharge. And / or, controlling the motor to move the reaction vessel to the chemiluminescence injection liquid level for liquid injection, and then controlling the motor to move the reaction vessel along the rotation direction to the nearest common drainage liquid level for liquid drainage.

10. A sample detection method, characterized in that, Comprising: Obtaining a detection mode corresponding to the sample; wherein, the detection mode includes at least one of flow cytometry fluorescence detection and chemiluminescence detection; In response to the detection mode being flow cytometry fluorescence detection, controlling the sensing module to be connected to the first circuit, and obtaining the detection result of the sample based on the analog signal output by the first circuit; In response to the detection mode being chemiluminescence detection, controlling the sensing module to be connected to the second circuit, and obtaining the detection result of the sample based on the digital signal output by the second circuit.

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