Driving mechanism, thermal cycle module, operation method of thermal cycle module and sample processing device

Abstract

The invention discloses a driving mechanism, a thermal cycle module, an operation method of the thermal cycle module and a sample processing device, and relates to the technical field of medical instruments and information intelligent equipment, and the driving mechanism, the thermal cycle module, the operation method of the thermal cycle module and the sample processing device provided by the invention specially aim at a detection scheme of top fluorescence collection. According to the scheme, through close cooperation of the horizontal driving mechanism and the lifting driving mechanism, convenient loading and unloading of the amplification consumables in the horizontal direction and efficient pressing in the vertical direction are achieved, and the stability of pressing force and the heat transfer efficiency are ensured. Due to the compact design and modular configuration of the thermal cycle module, a plurality of modules can run in parallel, and the high-throughput detection requirement is met. The device can effectively meet the requirements for processing a large number of samples and high automation in the modern molecular diagnosis technology, and the accuracy and reliability of detection are improved.

Description

Technical Field

[0001] The present invention relates to the technical fields of medical devices and information intelligent devices, and particularly relates to a driving mechanism, a thermal cycling module, an operation method thereof, and a sample processing device. Background Art

[0002] With the rapid development of molecular diagnostic technologies, especially their wide application in the field of disease diagnosis, technologies based on nucleic acid sequence fragment amplification have become increasingly popular and important in vitro diagnostic methods. Such technologies generally require the processing of biological samples to release nucleic acids, and the target sequences are amplified under the action of specific primers and enzymes. Through specific time or specific cycles, the nucleic acid sequences can be amplified exponentially, and the output of the quantitative amplification results is achieved through fluorescence labeling in cooperation with a detection module. Quantitative polymerase chain reaction (qPCR) is one of the most widely used detection technologies in current molecular diagnosis.

[0003] In recent years, various diseases such as respiratory-related diseases and intestinal diseases have occurred frequently. In vitro diagnostic technologies have been widely used in clinical diagnosis and treatment due to their advantage of minimal impact on the tested subjects. High-incidence disease periods are often accompanied by the need to process a large number of samples, and with the development of technology, the dependence on manual labor will gradually decrease. Therefore, simple, reliable, and highly automated operations are particularly important, which can not only reduce the risk of contamination but also reduce the requirements for the professionalism of operators.

[0004] In the early design of thermal cycling amplification modules, represented by European Patent EP1090141B1, an array of amplification consumable receiving blocks was designed, which could receive dozens of consumables with amplification systems, thus efficiently completing temperature cycling amplification. Although this design increased the number of samples processed, it had a relatively high requirement for manual participation and was suitable for the needs of rapid promotion and popularization in the initial stage of technology development.

[0005] Subsequently, US Patent US20100112683A2 disclosed a thermal lid unit and a control method that matches a thermal cycling temperature block. This thermal lid unit can keep the top of the amplification consumable at a target temperature of 94°C - 110°C, thus avoiding the problem of liquid condensation sticking to the wall during the amplification process and ensuring the reliable and accurate implementation of the detection scheme for top fluorescence collection. However, the design of such thermal lids is complex and has a relatively high requirement for the accuracy of thermal lid control, and they are relatively rare in practical applications.

[0006] In order to further improve the amplification efficiency, US Patent US10253361B2 proposed a negative pressure suction device, which enhances the heat transfer efficiency by introducing a negative pressure during amplification to make the amplification consumable closely fit with the thermal cycling temperature block. However, the equipment complexity of this solution is relatively high, and the requirement for negative pressure control is very precise, and it is rarely seen in actual use.

[0007] European Patent EP2976156B1 discloses a solution where the thermal lid is designed to be rotatably and snap - connected to the thermal block around an axis. The mechanical pressing force of the rotational snap - connection is used to apply a pressing effect on the amplification consumables inside the thermal block to ensure the amplification efficiency. However, this design is not conducive to the application of an automated operating system, mainly because it is very difficult to automate the modification of opening and closing the thermal lid, and there is a horizontal component of the force applied during the snap - connection process, which affects the fit between the amplification consumables and the thermal block.

[0008] The further improved US Patent US10850283B2 reserves an interface for detachable configuration of a temperature calibration plate. During use, by plugging in an external temperature calibration plate, the true heat transfer characteristics inside the thermal block can be accurately sensed, thereby improving the accuracy of the amplification results. However, this solution still requires manual intervention and is not fully suitable for the requirements of high automation.

[0009] The latest US Patent US11247211B2 designs a snap - connection motion solution for the thermal lid driven by a cam link. This solution not only realizes the snap - connection of the thermal lid, but also adds a vertical lifting motion to the snap - connection motion trajectory, so that the thermal lid first rotates to a certain height directly above the thermal block and then moves vertically downward to press the amplification consumables. Compared with the pure rotational snap - connection solution, the pressing force applied to the amplification consumables by this design has no horizontal component, and the reliability of pressing is higher. However, this design is also not applicable to the detection scheme of top - fluorescence collection because the vertical lifting motion of the thermal lid may interfere with the detection process.

[0010] In summary, although the thermal cycling amplification modules in the prior art have improved the amplification efficiency and reliability to a certain extent, there are still some deficiencies. Especially for the detection scheme that requires top - fluorescence collection, the prior art cannot fully meet the requirements of high - automation operation and performs poorly in efficiently processing a small number of samples. Therefore, it is particularly necessary to develop an amplification module that can adapt to top - fluorescence collection, while meeting the requirements of convenient addition of amplification consumables, being able to add a small amount at any time, and being able to reliably press the amplification consumables in an automated environment. Summary of the Invention

[0011] The object of the present invention is as follows: In view of the above problems, the present invention provides a driving mechanism, a thermal cycling module, its operation method and a sample processing device, which are specifically designed for the detection scheme of top fluorescence collection. Through the close cooperation of the horizontal driving mechanism and the lifting driving mechanism, this scheme realizes the convenient loading and unloading of the amplification consumables in the horizontal direction and the efficient pressing in the vertical direction, ensuring the stability of the pressing force and the heat transfer efficiency. The compact design and modular configuration of the thermal cycling module enable multiple modules to operate in parallel to meet the high-throughput detection requirements. This device can effectively cope with the large number of sample processing and high automation requirements in modern molecular diagnostic technologies, improving the accuracy and reliability of detection.

[0012] The technical solution adopted by the present invention is as follows:

[0013] A driving mechanism includes a horizontal driving mechanism, a lifting driving mechanism and a bearing substrate. The horizontal driving mechanism includes a horizontal driving device, a horizontal transmission device, a horizontal connecting block, a first transmission block and a first slide rail. The lifting driving mechanism includes a lifting driving device, a lifting transmission device and a lifting connecting block; One side of the bearing substrate is used for loading a moving target, and the other side is equipped with a first transmission block. One side of the first slide rail is connected to the horizontal driving device, and the other side is inserted into the first transmission block and is slidably connected to the first transmission block. The horizontal connecting block is connected to the bearing base surface, and the horizontal driving device can drive the horizontal connecting block to move horizontally along the horizontal transmission device; The lifting connecting block is connected to the first transmission block, and the lifting driving device can drive the lifting connecting block to move vertically along the lifting transmission device.

[0014] Further, the lifting transmission device includes a second driving lead screw, a second transmission block and a vertical slide rail; The lifting connecting block is slidably connected to the vertical slide rail. The second transmission block is provided with a fitting chute, and the lifting connecting block is provided with a pin column matching the fitting chute. At least part of the pin column is inserted into the fitting chute; The second transmission block is threadedly connected to the second driving lead screw. When the lifting driving device drives the second driving lead screw to rotate, it can drive the second transmission block to move along the length direction of the second driving lead screw. Relative movement can be generated through the fitting chute and the pin column, driving the lifting connecting block to move up and down along the vertical slide rail.

[0015] Further, the fitting chute includes an inclined section, and the inclined section is an inclined groove body forming a certain angle with the straight direction; When the pin column moves relative to the high position of the inclined section, it can drive the lifting connecting block to lift, and when the pin column moves relative to the low position of the inclined section, it can drive the lifting connecting block to descend.

[0016] Further, the fitting chute further includes a stable section communicated with the end of the inclined section. The stable section is a horizontal groove body extending from the end of the inclined section to the horizontal direction; When the pin column moves in the stable section, the height of the lifting connecting block remains unchanged.

[0017] Further, a support area is also provided on the carrier substrate. The support area and the first transmission block are oppositely arranged at both ends of the carrier substrate. At least two connecting parts are provided on the lifting connecting block. One connecting part is connected to the first transmission block, and a roller group that can be in rolling connection with the support area is arranged on the other connecting part.

[0018] Further, the horizontal transmission device includes a first driving lead screw, and the first driving lead screw is in threaded connection with the horizontal connecting block; the first driving lead screw is arranged in parallel with the second driving lead screw.

[0019] A thermal cycling module includes a thermal cycling module body. The driving mechanism as described above and a thermal lid module that can be matched with an amplification consumable are assembled in the thermal cycling module body. The moving target loaded on the carrier substrate is a thermal cycling component that can receive a plurality of amplification consumables and perform expansion operations on the amplification consumables. A horizontal driving limit structure and a lifting driving limit structure are also assembled in the thermal cycling module body. The thermal cycling module body restricts the horizontal displacement of the horizontal driving device along the horizontal direction through the horizontal driving limit structure. The lifting driving device moves along the horizontal direction under the action of the horizontal driving device with the carrier substrate. The thermal cycling component can extend out of or retract into the thermal cycling module body under the action of the horizontal driving mechanism; the thermal cycling module body restricts the vertical displacement of the lifting driving device along the vertical direction through the lifting driving limit structure. The horizontal driving device can move along the vertical direction under the action of the lifting driving mechanism with the carrier substrate. The thermal cycling component can be pressed against or separated from the thermal lid module under the action of the lifting driving mechanism.

[0020] Further, the thermal cycling component is flexibly connected to the carrier substrate through a plurality of elastic components. The elastic components include guide rods and elastic elements. One end of the guide rod is fixedly connected to the thermal cycling component, and the other end is movably inserted into the carrier substrate and limited by a retaining piece. The elastic element is sleeved on the guide rod and the elastic element is in a predetermined compressed state.

[0021] Further, the thermal cycling component of the thermal cycling module body includes an amplification consumable receiving part for receiving a plurality of amplification consumables. A temperature control device is arranged below the amplification consumable receiving part, and a radiator is arranged below the temperature control device; a plurality of amplification well units for receiving amplification consumables are linearly arranged on the amplification consumable receiving part; a plurality of positioning columns are also arranged on the amplification consumable receiving part. The thermal lid module includes a thermal lid substrate with a predetermined thickness. The bottom of the thermal lid substrate includes a heat capacity cavity that can accommodate the top of the amplification consumable and positioning holes that are used in cooperation with the positioning columns to achieve precise positioning of the thermal cycling component.

[0022] A sample processing device comprises a device body, wherein a plurality of thermal cycle modules and detection modules are assembled in the device body, a thermal cycle substrate is arranged on the top of the thermal cover module, a plurality of detection holes matching the thermal cavity are arranged on the thermal cycle substrate, and the detection module can perform extended detection on the corresponding amplification consumables through the detection holes.

[0023] A method for operating a thermal cycle module, applied to the thermal cycle module, comprises the following steps:

[0024] Amplification consumables loading step: the horizontal driving device drives the horizontal connecting block to move in the horizontal direction through the horizontal transmission device, drives the carrier substrate to extend to the amplification consumables receiving position outside the thermal cycle module body, puts the amplification consumables to be detected into the thermal cycle assembly, and then drives the thermal cycle assembly to retract in the horizontal direction to the amplification pressing position to be amplified by the horizontal driving device;

[0025] Thermal cover pressing step: the lifting drive device drives the lifting connection block to move in the vertical direction through the lifting transmission device, driving the horizontal driving mechanism and the carrier substrate to rise synchronously until the top of the amplification consumables matches the thermal cover module and generates a preset pressing force;

[0026] Amplification detection step: the thermal cycler and the thermal cover module heat the amplification consumables according to the settings to complete the amplification operation, and the detection module performs extended detection on the corresponding amplification consumables;

[0027] Amplification consumables unloading steps: the lifting drive device drives the lifting connection block to move in the vertical direction through the lifting transmission device, driving the horizontal driving mechanism and the carrying substrate to descend synchronously, so that the amplification consumables are separated from the thermal cover module; the horizontal drive device drives the horizontal connection block to move in the horizontal direction through the horizontal transmission device, driving the carrying substrate to extend to the amplification consumables receiving position outside the thermal cycle module body, and unloading the amplification consumables.

[0028] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0029] 1. The present invention decomposes the motion drive required by the thermal cycle component into: horizontal motion for receiving the amplification consumables, and vertical motion for cooperating with the thermal cover module. The two motions can be independently completed by the cooperation between the horizontal drive mechanism and the lifting drive mechanism. The horizontal drive mechanism drives the carrier substrate to move in the horizontal direction through the cooperation of the first drive screw and the horizontal connecting block, and follows the lifting of the carrier substrate through the cooperation of the first slide rail and the first transmission block, so that the lifting drive mechanism at a lower position can directly drive the horizontal drive mechanism and the carrier substrate to lift together through the first transmission block, so that the cooperation relationship in the component is basically not affected by the lifting motion.

[0030] 2. The first drive screw and the second drive screw of the present invention are arranged in parallel, so that two different motion drives can be reliably configured in a confined space, avoiding the problem that the module cannot be configured or is limited in configuration due to the need for a larger vertical space. Through the transmission cooperation of the screw slider, the rotational motion is converted into linear reciprocating motion, achieving high-precision and position drive control, and ensuring high-precision adjustment of motion in different directions.

[0031] 3. The present invention adopts a pin fixed on the lifting connection block to cooperate with the interlocking slide groove of the second transmission block, eliminating the relay transmission structure such as the connecting rod cam, and realizes the simplest conversion of the horizontal movement of the second transmission block along the second driving screw into the lifting movement of the lifting connection block along the vertical slide rail. This design improves the efficiency and reliability of motion conversion. The interlocking slide groove includes an inclined section and a stable section. The movement of the pin along the inclined section can change the height of the lifting connection block, and the movement of the pin along the stable section can maintain the height of the lifting connection block, ensuring that the thermal cover module applies sufficient and stable pressing force to the amplification consumables, ultimately improving the amplification efficiency. The vertical pressing operation can make the pressing force of the amplification consumables have no horizontal component, which will not affect the heat transfer efficiency.

[0032] 4. The sample processing device of the present invention can be equipped with multiple thermal cycle modules, each of which can operate independently and perform amplification detection in parallel. This design not only improves the detection efficiency, but also adapts to application scenarios with changes in detection flux. The detection module can be driven to perform linear reciprocating motion in the horizontal plane for detection, which makes the movement accuracy of the top detection module higher and the accuracy of the detection results higher.

[0033] 5. The present invention can reliably and flexibly complete amplification detection in batches in scenarios with a higher degree of automation. This design not only improves the degree of automation of the device, but also ensures the efficiency and stability of the detection process. It is particularly suitable for application in modern molecular diagnostic technology and can effectively meet the needs of large-scale sample processing and high-throughput detection.

[0034] 6. In conjunction with the operating method of the thermal cycle module of the present invention, the horizontal telescopic movement and the lifting movement can be driven by independent driving mechanisms. The horizontal driving device can drive the thermal cycle component as a whole to move in the horizontal plane to receive the amplification consumables with higher heat exchange efficiency, and then the lifting driving device only applies a compression force to the amplification consumables in the vertical direction to make the amplification efficiency higher, and by driving the parallel configuration of the driving screw rod, it can efficiently compress the amplification in a confined space. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 It is a schematic diagram of the overall structure of the thermal cycle module of the present invention;

[0036] Figure 2 is an exploded view of the thermal cycle assembly of the present invention;

[0037] Figure 3 It is a schematic structural view of the horizontal drive mechanism of the present invention in the retracted state;

[0038] Figure 4 It is a schematic structural view of the horizontal drive mechanism of the present invention in the extended state;

[0039] Figure 5 It is a schematic structural view of the lifting drive mechanism of the present invention in the lowered state;

[0040] Figure 6 It is a schematic structural view of the lifting drive mechanism of the present invention in the raised state;

[0041] Figure 7 It is a schematic structural view of the thermal cycling module of the present invention before receiving the amplification consumable and extending it along the horizontal plane;

[0042] Figure 8 It is a schematic structural view of the thermal cycling module of the present invention receiving the amplification consumable and extending it a certain distance along the horizontal plane;

[0043] Figure 9 It is a schematic structural view of the thermal cycling module of the present invention receiving the amplification consumable and extending it to the limit distance along the horizontal plane;

[0044] Figure 10 It is a schematic structural view of the thermal cycling module of the present invention before retracting after receiving the amplification consumable;

[0045] Figure 11 It is a schematic structural view of the thermal cycling module of the present invention during retracting after receiving the amplification consumable;

[0046] Figure 12 It is a schematic structural view of the thermal cycling module of the present invention after retracting after receiving the amplification consumable;

[0047] Figure 13 It is a schematic structural view of the thermal cycling component of the present invention being driven close to the heat capacity cavity;

[0048] Figure 14 It is a schematic structural view of the positioning post of the present invention extending into the positioning hole,

[0049] Figure 15 It is a schematic structural view of the thermal cycling component of the present invention being pressed against the heat cover module;

[0050] Figure 16 It is a schematic structural view of the sample processing device of the present invention.

[0051] Markings in the figure: 1 - Amplification consumables, 101 - Thermoelectric heating unit, 102 - Enhanced heat exchange unit, 103 - Compression sheet, 111 - Cooling fan, 112 - Cooling air duct, 113 - Radiator, 114 - Elastic component, 115 - Amplification consumables receiving part, 116 - Positioning post, 200 - Carrier substrate, 201 - Position sensor, 211 - Horizontal drive motor, 212 - First drive screw rod, 213 - Horizontal connection block, 214 - First slide rail, 215 - First drive block, 216 - Roller group, 221 - Lifting drive motor, 222 - Second drive screw rod, 223 - Second drive block, 2230 - Fitting chute, 224 - Lifting connection block, 2240 - Pin, 225 - Vertical slide rail, 30 - Thermal cover module, 301 - Detection sensor, 3001 - Detection hole, 311 - Heat capacity cavity, 312 - Positioning hole, 40 - Detection module, 410 - Thermal cycling substrate, 411 - Detection drive belt, 412 - Detection drive wheel, 413 - Detection driven wheel. Detailed implementation mode

[0052] The present invention will be described in detail below with reference to the accompanying drawings.

[0053] In order to make the purpose, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0054] Embodiment 1

[0055] A driving mechanism, as Figure 3-6 shown, includes a horizontal driving mechanism, a lifting driving mechanism and a carrier substrate 200. The horizontal driving mechanism includes a horizontal driving device, a horizontal transmission device, a horizontal connection block 213, a first drive block 215 and a first slide rail 214. The lifting driving mechanism includes a lifting driving device, a lifting transmission device and a lifting connection block 224. One side of the carrier substrate 200 is used for loading a moving target, and the other side is assembled with a first drive block 215. One side of the first slide rail 214 is connected to the horizontal driving device, and the other side is inserted into the first drive block 215 and is slidably connected to the first drive block 215. The horizontal connection block 213 is connected to the carrier base surface. The horizontal driving device can drive the horizontal connection block 213 to move horizontally through the horizontal transmission device. The lifting connection block 224 is connected to the first drive block 215. The lifting driving device can drive the lifting connection block 224 to move vertically through the lifting transmission device. Preferably, the horizontal driving device is a horizontal drive motor 211, and the lifting driving device is a lifting drive motor 221.

[0056] The lifting transmission device includes a second driving lead screw 222, a second transmission block 223, and a vertical slide rail 225; the lifting connection block 224 is slidably connected to the vertical slide rail 225. An engaging chute 2230 is provided on the second transmission block 223, and a pin 2240 matching the engaging chute 2230 is provided on the lifting connection block 224. The pin 2240 is at least partially inserted into the engaging chute 2230; the second transmission block 223 is threadedly connected to the second driving lead screw 222. When the lifting driving device drives the second driving lead screw 222 to rotate, it can drive the second transmission block 223 to move along the length direction of the second driving lead screw 222. Relative movement can be generated between the engaging chute 2230 and the pin 2240, driving the lifting connection block 224 to move up and down along the vertical slide rail 225.

[0057] The engaging chute 2230 includes an inclined section, which is an inclined groove body at a certain angle to the straight direction. The inclination angle of the inclined section can be controlled according to requirements to achieve different transmission ratios; when the pin 2240 moves to the high position of the inclined section, it can drive the lifting connection block 224 to lift, and when the pin 2240 moves to the low position of the inclined section, it can drive the lifting connection block 224 to descend.

[0058] The engaging chute 2230 further includes a stable section communicating with the end of the inclined section. The stable section is a horizontal groove body extending from the end of the inclined section to the horizontal direction; when the pin 2240 moves in the stable section, the height of the lifting connection block 224 remains unchanged.

[0059] A support area is further provided on the bearing substrate 200. The support area and the first transmission block 215 are respectively located at both ends of the bearing substrate 200. At least two connecting parts are provided on the lifting connection block 224. One connecting part is connected to the first transmission block 215, and a roller group 216 that can be in rolling connection with the support area is provided on the other connecting part.

[0060] Further, the horizontal transmission device is a first driving lead screw 212, and the first driving lead screw 212 is threadedly connected to the horizontal connection block 213; the first driving lead screw 212 is arranged in parallel with the second driving lead screw 222.

[0061] Embodiment 2

[0062] Figure 1This is a schematic diagram of the overall structure of the thermal cycling module of the present invention; at the topmost position, there is an amplification consumable receiving part 115, which includes several linearly arranged amplification well units to receive the amplification consumable 1. Here, it is shown that the amplification consumable receiving part 115 composed of eight linearly arranged amplification well units can thus receive multiple single amplification tubes or the more commonly used eight-well tube amplification consumables at present. Of course, the number of amplification well units can be other numbers. The linearly arranged amplification well units are more sensitive to the direction of the applied pressing force but require less magnitude of the applied pressing force, and can more easily balance the pressing force differences among different well units. Several positioning posts 116 are arranged outside the amplification consumable receiving part 115 to ensure more accurate pressing operations of the thermal cycling module. A radiator 113 is arranged below the amplification consumable receiving part 115. The radiator 113 is a fin-type radiator, obtained by the process of milling teeth. The fins can be arranged at equal intervals or configured as non-equal interval types to ensure sufficient heat dissipation efficiency. In order to ensure higher heat dissipation efficiency and have little impact on other functional units during heat dissipation operation, a heat dissipation air duct 112 is arranged around the radiator 113. Both ends of the heat dissipation air duct 112 are provided with openings. One end of the opening is communicated with the radiator 113, and the other end opening is communicated with the heat dissipation fan 111. The fins of the radiator 113 are arranged at intervals at the opening of the heat dissipation air duct 112, so that the air flow can be driven to flow along the length direction of the heat dissipation air duct 112 to achieve timely removal of waste heat. Here, the number of heat dissipation fans 111 connected to the heat dissipation air duct 112 can be one, two or more. The above functional units constitute a thermal cycling component capable of performing thermal cycling amplification. In order to ensure that the thermal cycling component is reliably and efficiently driven, the thermal cycling component is connected to the carrier substrate 200. Here, in order to ensure that the amplification consumable 1 in the amplification consumable receiving part 115 is not overly pressed and deformed, several elastic components 114 are used to connect the thermal cycling component and the carrier substrate 200. To ensure the reliability of the elastic connection, the number of the elastic components 114 is four. The elastic component 114 includes a guide rod and an elastic element. One end of the guide rod is fixedly connected to the thermal cycling component, and the other end is movably inserted into the carrier substrate 200 and limited by a retaining piece. The elastic element is threaded through the guide rod and is in a predetermined compressed state. Here, the elastic element is preferably a threaded spring and has an elastic coefficient of 0.9 - 1.3 N / mm. In this way, the thermal cycling component and the carrier substrate 200 can be basically reliably constrained and defined, and there will be no unreliable connection problems such as shaking or skew of the thermal cycling component due to the configuration of the elastic element.At the lower part of the thermal cycling component, a horizontal driving mechanism and a lifting driving mechanism are arranged in the height direction. Here, the horizontal driving mechanism is arranged at a higher position. The horizontal driving mechanism includes a horizontal driving motor 211, and the lifting driving mechanism includes a lifting driving motor 221. The output of the horizontal driving motor 211 is connected to the carrier substrate 200 carrying the thermal cycling component. In this way, the horizontal driving motor 211 drives the thermal cycling component to move horizontally to the amplification consumable receiving position or the to-be-amplified clamping position. The lifting driving motor 221 arranged at a lower height can output and connect the lifting connection block 224 of the horizontal driving mechanism and the carrier substrate 200 to perform lifting motion, thereby driving the thermal cycling component and the horizontal driving mechanism to lift and lower synchronously.

[0063] Figure 2 is an exploded view of the thermal cycling component of the present invention; the amplification consumable receiving part 115 includes eight amplification well units arranged in a line. The amplification well units arranged in a line can be adapted to the detection module driven linearly, so as to efficiently and quickly obtain the amplification results in the amplification consumables 1 received by multiple amplification well units under the simplified motion driving state. In order to increase its heat capacity, the bottoms of the amplification well units are connected and combined by connectors. In order to enhance the heat exchange efficiency, the bottoms of the amplification well units are also provided with enhanced heat exchange units 102, which are indirectly connected to the thermoelectric heating unit 101 through the enhanced heat exchange units 102. Here, the thermoelectric heating unit 101 is used as the heat source of the amplification consumable receiving part 115, which can meet the dual requirements of rapid heating and rapid cooling, making the cyclic amplification execution efficiency higher. In order to more reliably fixedly connect the amplification consumable receiving part 115 to the radiator 113, a pressing piece 103 is also provided above the amplification consumable receiving part 115. The pressing piece 103 and the pressing screw can reliably fixedly connect the amplification consumable receiving part 115 to the radiator 113. In this embodiment, the number of the cooling fans 111 in the thermal cycling component is one. In this way, the operation control method of the cooling fan 111 can be more optimally matched, and whether there is an operation failure can be detected in time, and the maintenance and replacement are more convenient. In order to reduce the flow dissipation of the cooling air flow, the cooling air duct 112 includes a transition section with a gradually decreasing cross-sectional area, and also includes a steady flow section with a substantially consistent cross-sectional area. The cooling fins of the radiator 113 are all arranged in the steady flow section. In this way, on the one hand, the air flow velocity passing through the cooling fins can be reduced to ensure more sufficient heat exchange, and on the other hand, the dissipation effect in the steady flow section is smaller, making the flow field more stable and almost no flow dead zone exists.

[0064] Figure 3 is a schematic structural diagram of the horizontal driving mechanism of the present invention in the retracted state. Figure 4It is a schematic structural diagram of the horizontal drive mechanism of the present invention in the extended state; driven by the horizontal drive motor 211, the carrier substrate 200 can drive the connected thermal cycle assembly to move to different positions. Here, the lifting drive mechanism at a lower position is omitted and the bottom of the thermal cycle assembly is used as the perspective. In this embodiment, the horizontal drive motor 211 is connected to and can drive the first drive lead screw 212 to rotate. The first drive lead screw 212 is in threaded engagement with the horizontal connection block 213. The horizontal connection block 213 is fixedly connected to the carrier substrate 200. One side of the carrier substrate 200 is connected to the thermal cycle assembly, and the other side is provided with a first transmission block 215. One side of the first slide rail 214 is connected to the horizontal drive motor 211, and the other side is slidably connected to the first transmission block 215. Here, the first transmission block 215 is fixedly connected to the lifting connection block 224 that can move up and down. Thus, when the horizontal drive motor 211 outputs a rotational motion, it can drive the entire carrier substrate 200 to move with high precision under the constraint of the first transmission block 215. In this motion drive, it can ensure that the relative position between the carrier substrate 200 and the thermal cycle assembly carried thereon is fixed, making the reference of the thermal cycle assembly more constant. Along the width direction of the thermal cycle assembly, a support area is also provided on the carrier substrate 200 at a preset interval from the first transmission block 215. The support area is in rolling contact with the roller group 216 fixedly connected to the lifting connection block 224. Here, the support area can directly be at least part of the other side of the carrier substrate 200 relative to the side connected to the first transmission block 215. The part serving as the support area can have higher machining accuracy, or the entire bottom of the carrier substrate 200 has higher machining accuracy. The support area is in rolling contact with the roller group 216. The distance between the support area contacted by the roller group 216 and the first transmission block 215 is less than the width of the thermal cycle assembly. The distance between the two can be configured to be 0.7 - 0.95 times the width dimension of the thermal cycle assembly. The carrier substrate 200 is indirectly connected to the lifting connection block 224 through two different connection methods of fitting and sliding and rolling contact. The two methods have a low risk of interference during the driving motion of the carrier substrate 200, can be reliably arranged in a narrower space, and can also introduce the relatively flexible constraint support of the rolling contact of the roller group 216. So that even when the carrier substrate 200 is assembled with a skew or there is a skew during long-term use and other situations that generate stronger constraints, it can adaptively self-adjust and still ensure that the carrier substrate 200 is driven to move reliably with low resistance. In order to ensure the reliability of the support of the roller group 216 for the carrier substrate 200 and its low-resistance operation, the roller group 216 includes two roller units, and the two roller units are arranged at a preset interval.

[0065] Figure 5 It is a schematic structural diagram of the lifting drive mechanism of the present invention in the lowered state. Figure 6This is a schematic structural diagram of the lifting drive mechanism of the present invention in the raised position. The two figures show the process diagrams of the lifting drive mechanism driving the lifting connection block 224 to move to different heights. The lifting drive mechanism is arranged at a lower position below the horizontal drive mechanism. The power source of the lifting drive mechanism is the lifting drive motor 221. The lifting drive motor 221 is connected and can drive the second drive screw 222 to rotate. Here, the axis of the second drive screw 222 is parallel to the axis of the first drive screw 212, so that both are rotational movements, and there is basically no risk of mutual interference during operation. Such a configuration occupies less space, is more convenient for subsequent maintenance and repair, and there is no need to reserve a space for the intersecting drive screws, thus avoiding the problem of changes in the amplification reaction conditions of the thermal cycling components performing thermal cycling amplification. The second drive screw 222 is threadedly engaged with a second transmission block 223. A fitting chute 2230 can be formed on the second transmission block 223 by machining methods such as milling. A pin column 2240 is fixedly connected to the lifting connection block 224, and at least part of the pin column 2240 is inserted into the fitting chute 2230. Here, in order to reduce the movement resistance of the part of the pin column 2240 inserted into the fitting chute 2230, a pin wheel can be assembled at the end of the pin column 2240. In this way, the pin wheel can rollingly contact the fitting chute 2230 to greatly reduce the transmission resistance. A plurality of vertical sliders are also fixedly connected to the end of the lifting connection block 224. The lifting connection block 224 can be slidably connected to the vertically fixed vertical slide rail 225 through the vertical sliders. Here, in order to ensure the low-resistance, reliable and stable characteristics of the vertical sliding movement, the number of vertical sliders can be configured to be not less than two. Of course, the number of corresponding vertical slide rails 225 is the same and not less than two. At least two connecting parts are configured on the top of the lifting connection block 224. One connecting part is fixedly connected to the first transmission block 215, and the other connecting part is fixedly connected to the roller group 216. The height of the connection surface between the connecting roller group 216 and the connecting part is lower than the connection surface between the connecting first transmission block 215 and the connecting part. In this way, it can adapt to the spatial requirement differences of the two different connection methods. The roller group 216 also has sufficient dimensions to ensure the reliability of support during long-term use, and the risk of wear and damage is smaller. The lifting connection block 224 is also directly or indirectly connected to the horizontal drive motor 211. In this embodiment, the lifting drive motor 221 can drive the second drive screw 222 to rotate. The second transmission block 223 threadedly engaged with it can be driven to slide along the axis direction of the second drive screw 222. When the second transmission block 223 is driven to approach the lifting drive motor 221, the fitting chute 2230 on the second transmission block 223 is driven to move, so that at least part of the inserted pin column 2240 and the fitting chute 2230 generate relative movement. The fitting chute 2230 is configured to include an inclined section at a certain angle to the vertical direction, and stable sections extending in the horizontal direction are respectively provided at both ends in the length direction of the inclined section. The relative movement of the pin column 2240 in the inclined section will gradually lift the height of the pin column 2240, and thus can lift the height of the lifting connection block 224.The height of the supporting substrate 200 and the horizontal driving motor 211 connected thereto can also be lifted together, so that the height of the supporting substrate 200 can be changed while ensuring the compact arrangement of the device. At the same time, the angle setting of the inclined section can be used to set the transmission ratio of the lifting driving motor 221 as required, so that the vertical lifting motion control accuracy is higher, and the configuration of the terminal stabilizing section enables the lifting motion drive to have sufficient buffering, and can also accurately and reliably receive the pin 2240, ensuring that the final clamping force applied is more constant. This pin 2240 cooperates with the engaging groove 2230 of the second transmission block 223, eliminating the relay transmission structure such as the connecting rod cam, and realizes the most simplified conversion of the horizontal sliding of the second transmission block 223 on the second driving screw 222 into the lifting motion of the lifting connection block 224 along the vertical slide rail 225, with higher motion conversion efficiency and reliability, and can also adapt to the precise and reliable transmission scenario of multiple amplification well units arranged linearly for small clamping force values. When the second transmission block 223 is driven away from the lifting driving motor 221, the movement is opposite, which will not be repeated here.

[0066] Figure 7 It is a schematic diagram of the structure of the thermal cycle module of the present invention before it extends along the horizontal plane after receiving the amplification consumables. Figure 8 The thermal cycle module of the present invention is a schematic diagram of the structure of the amplification consumables extending along the horizontal plane at one end. Figure 9 It is a structural schematic diagram of the thermal cycle module of the present invention that receives the amplification consumables and extends them to the limit distance along the horizontal plane. Here is a diagram of the horizontal drive mechanism driving the thermal cycle component to move in the horizontal plane after the horizontal drive mechanism, the lifting drive mechanism and the thermal cycle component are combined. The horizontal drive motor 211 outputs a rotational motion to drive the first drive screw 212 to rotate. The threaded horizontal connecting block 213 converts the rotational motion into a linear motion along the axis of the first drive screw 212 through transmission cooperation. The horizontal connecting block 213 is fixedly connected to the carrier substrate 200, so that the carrier substrate 200 can be driven to slide in the horizontal plane. In order to ensure the accuracy of the horizontal motion position, a sensor sheet is also configured at the bottom of the carrier substrate 200, and a position sensor 201 is arranged at a specific position on the horizontal plane to serve as a positioning reference or a limit position limiter. The clockwise or counterclockwise rotation of the horizontal drive motor 211 can drive the carrier substrate 200 to different positions on the horizontal plane to cooperate in performing different functional operations.

[0067] Figure 10 is a schematic diagram of the structure of the thermal cycle module of the present invention after receiving the amplification consumables and before retracting. Figure 11 It is a schematic diagram of the structure of the thermal cycle module of the present invention retracting after receiving the amplification consumables. Figure 12It is a schematic structural diagram after the thermal cycling module of the present invention retracts after receiving the amplification consumable. Above the thermal cycling component, a thermal cover module 30 is provided. The thermal cover module 30 can cooperate with the thermal cycling component at a set position. The thermal cover module 30 includes a thermal cover substrate with a predetermined thickness. The bottom of the thermal cover substrate includes a heat capacity cavity 311 for accommodating the top of the amplification consumable 1 and a positioning hole 312 for cooperating with the positioning post 116 to achieve precise positioning of the thermal cycling component. The heat capacity cavity 311 can reliably limit the amplification consumable 1 and can provide a relatively closed environment, enabling the top of the amplification consumable 1 to be in a relatively more stable thermal environment, reducing the possible condensation risk during the amplification process. The top of the thermal cover substrate is configured with a detection module 40 that can be driven to perform linear reciprocating motion in the horizontal plane. The detection module 40 can be designed to include any number of detection channels. Here, in order to achieve more types of target detections, the detection module 40 is preferably included with different wavelength detection channels of four channels, five channels, six channels, seven channels, or even eight channels. Of course, in order to precisely control or position the detection module 40, a detection sensor 301 is fixedly connected to a specific position on the top of the thermal cover module, and a detection sensing piece is fixedly connected to the detection module 40 in cooperation with it. Before performing the amplification, the horizontal drive motor 211 can output a rotational motion to drive the carrier substrate 200 and the thermal cycling component connected thereto to move horizontally. As Figure 10 shown, the horizontal drive motor 211 drives the thermal cycling component to move to the amplification consumable receiving position to receive the amplification consumable 1 containing the amplification system liquid transferred by an artificial or automatic robotic arm. During this process, the thermal cycling component is driven as a whole, so that the state of receiving the amplification consumable 1 basically does not change during subsequent movements, making the amplification efficiency higher. After receiving the amplification consumable 1, the horizontal drive motor 211 rotates in the opposite direction to drive the thermal cycling component to retract and finally move to the horizontal position to be pressed for amplification. As Figure 12 shown, at this time, the amplification consumable 1 is located directly below the heat capacity cavity 311, and the positioning post 116 is located directly below the positioning hole 312. The horizontal drive motor 211 can stop operating and then perform the lifting and pressing operation.

[0068] Figure 13 It is a schematic structural diagram of the thermal cycling component of the present invention being driven close to the heat capacity cavity. Figure 14 It is a schematic structural diagram of the positioning post extending into the positioning hole. Figure 15It is a schematic structural diagram of a thermal cycling component pressed against a thermal cover module. After the thermal cycling component drives the amplification consumable 1 to move to the amplification position to be pressed, the lifting drive motor 221 at a lower position can output a clockwise or counterclockwise rotational motion, thereby driving the second drive screw rod 222 parallel to the first drive screw rod 212 to rotate around the axis. The second transmission block 223 connected by screw thread can convert the rotational motion into a linear motion along the axis direction of the second drive screw rod 222, and then cooperate with the fitting chute 2230 and the pin 2240 at least partially inserted therein to achieve the effects of changing the linear motion direction and transmission ratio. In this way, the thermal cycling component is driven to move in the vertical direction, so that the top of the amplification consumable 1 approaches and contacts the thermal cover module 30. Since the thermal cycling component is elastically connected to the carrier substrate 200, after the top of the amplification consumable 1 contacts the thermal cover module 30, a gradually increasing pressing force can be applied, so that the amplification consumable 1 is pressed between the thermal cycling component and the thermal cover module 30 without the risk of deformation. Finally, as Figure 15 shown, the pin 2240 can be fitted into the stable section of the fitting chute 2230 to ensure the pressing force and accurately lock the pressing state at the same time. In the pressing state, the thermoelectric heating unit 101 in the thermal cycling component performs a cyclic heating and cooling operation according to the set program, so that the amplification reaction system liquid in the amplification consumable 1 performs amplification, and the detection module 40 at the top is reciprocally driven to obtain fluorescence results in different amplification cycles to complete the qualitative or quantitative detection of the target. After the detection is completed, the lifting drive motor 221 rotates in reverse, so that the top of the amplification consumable 1 moves away from and separates from the thermal cover module 30.

[0069] Embodiment 3

[0070] A sample processing device, such as Figure 16As shown, it includes a device body, and any number of thermal cycle modules provided in Example 2 can be installed in the device body. In order to achieve a larger module detection flux and higher detection flexibility, six thermal cycle modules are installed in the device body, respectively marked as N1-N6, and each thermal cycle component includes an independent horizontal drive mechanism and a lifting drive mechanism. Multiple lifting drive motors 221 can lift or lower the vertical position of the amplification consumables 1 carried therein, so that each thermal cycle component can be controlled to operate independently, and an unsaturated working state in which some thermal cycle components are running and some are not running can be achieved. It can also be achieved that during the amplification process of some thermal cycle components, the thermal cycle components that do not carry the amplification consumables are configured without interference with the amplification consumables 1, so as to adapt to the operation scenario of the detection amount change, so that the module is more flexible. The device body is also configured with a detection module 40, and a thermal cycle substrate 410 is provided on the top of the thermal cover module 30. The thermal cycle substrate 410 is configured with a plurality of detection holes 3001 matching the thermal cavity 311, and the detection module 40 can perform extended detection on the corresponding amplification consumables 1 through the detection holes 3001. In this embodiment, the detection holes 3001 are arranged linearly to correspond to the amplification consumables 1 received in the amplification consumables receiving part 115 of each thermal cycle component. A detection drive mechanism is arranged on the top of the thermal cycle substrate 410, which includes a detection drive motor, whose output shaft is connected to the detection drive wheel 412, and a detection driven wheel 413 is arranged at a preset interval therebetween, and a detection transmission belt 411 is wound between the two. A detection module 40 is fixedly connected to one side of the detection transmission belt 411. The detection drive motor drives the detection module 40 to move reciprocatingly in a straight line on the top of the thermal cycle substrate 410 to achieve detection. In order to accurately limit the movement range of the detection module 40, two spaced-apart detection sensors 301 are also arranged on the top of the thermal cycle substrate 410, and the two detection sensors 301 correspond to the two extreme positions of the moving direction of the detection module 40 respectively.

[0071] Example 4

[0072] A method for operating a thermal cycle module, applied to the thermal cycle module in Example 2, comprises the following steps:

[0073] Amplification consumable 1 loading step: the horizontal driving device drives the horizontal connecting block 213 to move in the horizontal direction through the horizontal transmission device, driving the carrier substrate 200 to extend to the amplification consumable receiving position outside the thermal cycle module body, placing the amplification consumable 1 to be detected into the thermal cycle assembly, and then the horizontal driving device drives the thermal cycle assembly to retract in the horizontal direction to the amplification pressing position to be amplified;

[0074] Thermal cover pressing step: the lifting drive device drives the lifting connection block 224 to move in the vertical direction through the lifting transmission device, driving the horizontal driving mechanism and the carrier substrate 200 to rise synchronously until the top of the amplification consumable 1 matches the thermal cover module 30 and generates a preset pressing force;

[0075] Amplification detection step: The thermal cycling component and the thermal cover module 30 heat the amplification consumable 1 according to the set temperature to complete the amplification operation, and the detection module 40 performs extended detection on the corresponding amplification consumable 1.

[0076] Amplification consumable 1 unloading step: The lifting drive device drives the lifting connection block 224 to move in the vertical direction through the lifting transmission device, driving the horizontal drive mechanism and the carrier substrate 200 to descend synchronously, so that the amplification consumable 1 is separated from the thermal cover module 30. The horizontal drive device drives the horizontal connection block 213 to move in the horizontal direction through the horizontal transmission device, driving the carrier substrate 200 to extend to the amplification consumable 1 receiving position outside the thermal cycling module body, and unloading the amplification consumable 1.

[0077] Specific embodiments are used in this article to elaborate on the principles and implementation manners of the present invention. The descriptions of the above embodiments are only used to help understand the method and its core idea of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and modifications can be made to the present invention, and these improvements and modifications also fall within the protection scope of the claims of the present invention.

[0078] In the description of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship in which the product of the present invention is usually placed during use. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation to the present invention.

[0079] In the description of the present invention, it should also be noted that unless otherwise clearly specified and limited, the terms "set", "installed", "connected", "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

Claims

1. A driving mechanism, characterized in that, It includes a horizontal driving mechanism, a lifting driving mechanism and a carrying substrate. The horizontal driving mechanism includes a horizontal driving device, a horizontal transmission device, a horizontal connecting block, a first transmission block and a first slide rail. The lifting driving mechanism includes a lifting driving device, a lifting transmission device and a lifting connecting block. One side of the carrying substrate is used for loading a moving target, and the other side is assembled with the first transmission block. One side of the first slide rail is connected to the horizontal driving device, and the other side is inserted into the first transmission block and is slidably connected to the first transmission block. The horizontal connecting block is connected to the carrying base surface, and the horizontal driving device can drive the horizontal connecting block to move horizontally along the horizontal direction through the horizontal transmission device. The lifting connecting block is connected to the first transmission block, and the lifting driving device can drive the lifting connecting block to move vertically along the vertical direction through the lifting transmission device.

2. The drive mechanism according to claim 1, characterized in that, The lifting transmission device includes a second driving lead screw, a second transmission block and a vertical slide rail. The lifting connecting block is slidably connected to the vertical slide rail. The second transmission block is provided with a fitting chute, and the lifting connecting block is provided with a pin column matching the fitting chute. At least part of the pin column is inserted into the fitting chute. The second transmission block is threadedly connected to the second driving lead screw. When the lifting driving device drives the second driving lead screw to rotate, it can drive the second transmission block to move along the length direction of the second driving lead screw. Through the relative movement between the fitting chute and the pin column, it can drive the lifting connecting block to move up and down along the vertical slide rail.

3. The drive mechanism according to claim 2, wherein, The fitting chute includes an inclined section, and the inclined section is an inclined groove body at a certain angle to the vertical direction. When the pin column moves to the high position of the inclined section, it can drive the lifting connecting block to lift. When the pin column moves to the low position of the inclined section, it can drive the lifting connecting block to descend.

4. The drive mechanism according to claim 3, characterized in that, The fitting chute further includes a stable section communicating with the end of the inclined section. The stable section is a horizontal groove body extending horizontally from the end of the inclined section. When the pin column moves in the stable section, the height of the lifting connecting block remains unchanged.

5. The drive mechanism according to claim 4, characterized in that The carrying substrate is further provided with a support area. The support area and the first transmission block are relatively arranged at both ends of the carrying substrate. The lifting connecting block is provided with at least two connecting parts. One connecting part is connected to the first transmission block, and the other connecting part is provided with a roller group that can be in rolling connection with the support area.

6. The drive mechanism according to any one of claims 2-5, characterized in that, The horizontal transmission device includes a first driving lead screw. The first driving lead screw is threadedly connected to the horizontal connecting block. The first driving lead screw is arranged parallel to the second driving lead screw.

7. A thermal cycling module, comprising a thermal cycling module body, characterized in that, The thermal cycle module body is equipped with a driving mechanism as described in any one of claims 1 to 6, and a thermal cover module that can be matched with an amplification consumable. The moving target loaded on the carrier substrate is a thermal cycle component that can undertake a number of amplification consumables and can perform expansion operations on the amplification consumables. The thermal cycle module body is also equipped with a horizontal drive limiting structure and a lifting drive limiting structure. The thermal cycle module body limits the horizontal displacement of the horizontal drive device through the horizontal drive limiting structure, and the lifting drive device moves in the horizontal direction with the carrier substrate under the action of the horizontal drive device. The thermal cycle component can extend or retract the thermal cycle module body under the action of the horizontal drive mechanism; the thermal cycle module body limits the vertical displacement of the lifting drive device through the lifting drive limiting structure, and the horizontal drive device can move in the vertical direction with the carrier substrate under the action of the lifting drive mechanism. The thermal cycle component can press or detach from the thermal cover module under the action of the lifting drive mechanism.

8. The thermal cycling module according to claim 7, wherein The thermal cycle component is flexibly connected to the carrier substrate through a plurality of elastic components, the elastic component comprises a guide rod and an elastic element, one end of the guide rod is fixedly connected to the thermal cycle component, and the other end is movably plugged into the carrier substrate and limited by a baffle, the elastic element is passed through the guide rod and puts the elastic element in a predetermined compression state; the thermal cycle component comprises an amplification consumable receiving part for receiving a plurality of amplification consumables, a temperature control device is arranged below the amplification consumable receiving part, and a radiator is arranged below the temperature control device; the amplification consumable receiving part is linearly arranged with a plurality of amplification well units for receiving amplification consumables; a plurality of positioning columns are also arranged on the amplification consumable receiving part, the thermal cover module comprises a thermal cover substrate with a predetermined thickness, the bottom of the thermal cover substrate comprises a heat capacity cavity for accommodating the top of the amplification consumables and a positioning hole used in conjunction with the positioning column to achieve precise positioning of the thermal cycle component.

9. A sample processing device, comprising a device body, characterized in that, The device body is equipped with a plurality of thermal cycle modules and detection modules according to any one of claims 7 to 8, a thermal cycle substrate is provided on the top of the thermal cover module, and a plurality of detection holes matching the thermal cavity are arranged on the thermal cycle substrate, and the detection module can perform extended detection on the corresponding amplification consumables through the detection holes.

10. A method for operating a thermal cycling module, applied to the thermal cycling module according to any one of claims 7-8, characterized in that, The steps include: Amplification consumables loading step: the horizontal driving device drives the horizontal connecting block to move in the horizontal direction through the horizontal transmission device, drives the carrier substrate to extend to the amplification consumables receiving position outside the thermal cycle module body, puts the amplification consumables to be detected into the thermal cycle assembly, and then drives the thermal cycle assembly to retract in the horizontal direction to the amplification pressing position to be amplified by the horizontal driving device; Thermal cover pressing step: the lifting drive device drives the lifting connection block to move in the vertical direction through the lifting transmission device, driving the horizontal driving mechanism and the carrier substrate to rise synchronously until the top of the amplification consumables matches the thermal cover module and generates a preset pressing force; Amplification detection step: the thermal cycler and the thermal cover module heat the amplification consumables according to the settings to complete the amplification operation, and the detection module performs extended detection on the corresponding amplification consumables; Amplification consumable unloading step: The lifting drive device drives the lifting connection block to move in the vertical direction through the lifting transmission device, driving the horizontal drive mechanism and the carrier substrate to descend synchronously, so that the amplification consumable is separated from the hot cover module. The horizontal drive device drives the horizontal connection block to move in the horizontal direction through the horizontal transmission device, driving the carrier substrate to extend to the amplification consumable receiving position outside the thermal cycling module body, and unloading the amplification consumable.

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