Arthrophlogosis stem cell therapeutic drug screening device and using and screening method thereof
Through an integrated arthritis stem cell therapy drug screening device, the arthritis pathological environment is simulated and the impact of drugs on chondrocytes is evaluated, which solves the problems of long and high cost of drug screening in the prior art, and achieves efficient and low-cost drug screening and treatment effect evaluation.
Patent Information
- Application Number
- CN202510314750.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-18
- Publication Date
- 2025-07-04
AI Technical Summary
The arthritis treatment model now has the problem of long drug screening cycle, high cost, and inability to simulate complex environments in the body. The data collection and analysis are incomplete, resulting in large differences in the treatment effects of stem cell and the inability to achieve personalized and precise treatment.
Design an integrated and intelligent drug screening device for arthritis stem cell therapy, including cell culture module, drug processing module, organ chip simulation module and data analysis module. By simulating the arthritis pathological environment, the impact of drugs on chondrocytes is evaluated, experimental data is collected and analyzed, and the drug combination is optimized.
It improves the efficiency of drug screening, reduces costs, and accelerates the drug development process. It can more accurately evaluate the therapeutic effect of drugs on chondrocytes, promotes the self-repair and regeneration ability of stem cells, and repairs damaged joint tissue.
Smart Images
Figure CN120249051A_ABST
Abstract
Description
Technical Field
[0001] The present invention belongs to the field of medical devices, and particularly relates to an arthritis stem cell therapy drug screening device and its use and screening method. Background Art
[0002] Arthritis is one of the relatively common chronic diseases clinically, with a high disability rate. Patients will suffer long-term pain and a huge medical burden. At present, the main treatment methods for arthritis include drug treatment, physical therapy, surgical treatment, etc., but the treatment effects are not satisfactory. Stem cell therapy is a newly emerging arthritis treatment method in recent years. By injecting stem cells into patients, damaged chondrocytes can be activated and repaired, the growth of new cartilage tissue can be promoted, and the inflammatory response can be inhibited, so as to achieve the purpose of treating arthritis. Although animal experiments and preliminary clinical studies have shown that stem cell therapy shows great potential in the treatment of arthritis, due to factors such as large individual differences among arthritis patients, high disease heterogeneity, non-standard treatment methods, and imperfect efficacy evaluation, there are significant differences in the treatment effects of stem cells. Therefore, developing an efficient, reliable, low-cost and easy-to-operate arthritis treatment drug screening platform and treatment method will help to reveal the pathogenesis of arthritis, improve the treatment effect of stem cells, improve the treatment efficiency and success rate, and promote the development of personalized precision treatment for arthritis.
[0003] The existing models for discovering and validating arthritis stem cell therapy are mainly divided into in vitro models and in vivo models. In vitro models are mainly based on in vitro stem cell culture systems, but they have the disadvantages of long drug screening cycles, high costs, and inability to simulate the complex in vivo environment. In vivo models include animal models and human models. The application of animal models and human models has some limitations. For example, the experimental results of animal models may not be directly applied to humans, the disease phenotypes of animal models are quite different from human arthritis, the costs of animal experiments are relatively high, and a large number of ethical reviews and regulatory procedures are required. In addition, the data collection and analysis of the above models mainly rely on manual operations, with problems such as long data collection cycles, large amounts of data, and imperfect data collection and analysis, and it is impossible to comprehensively evaluate the treatment effect of stem cells on arthritis.
[0004] Therefore, the present invention proposes an integrated and intelligent arthritis stem cell therapy drug screening device and method to solve the above problems. Summary of the Invention
[0005] In view of one or more of the above defects or improvement requirements of the prior art, the present invention provides an apparatus for screening drugs for the treatment of arthritis with stem cells, as well as a method for using and screening the same. By amplifying, differentiating stem cells and maintaining the physiological state of stem cells, the drug is directly mixed with stem cells in a specific ratio to simulate the pathological environment of arthritis, evaluate the effect of the drug on chondrocytes, collect and analyze experimental data, so as to confirm and optimize the best drug combination, reduce the number of animal models required for traditional tests, improve the drug screening efficiency, reduce costs, and accelerate the drug R & D process.
[0006] To achieve the above object, the present invention provides an apparatus for screening drugs for the treatment of arthritis with stem cells, which includes a cell culture module, a drug treatment module, an organ chip simulation module and a data analysis module;
[0007] The cell culture module is used for amplifying, differentiating stem cells and maintaining the physiological state of stem cells; the drug treatment module is used for precisely adding the drug to be screened into the stem cell culture system and controlling the concentration and action time of the drug; the organ chip simulation module is used for simulating the pathological environment of arthritis and evaluating the effect of the drug on chondrocytes; the data analysis module is used for collecting and analyzing the experimental data in the organ chip simulation module and evaluating the potential therapeutic effect of the drug.
[0008] The organ chip simulation module includes a cartilage organ chip, a mechanical stress loading system and an inflammatory factor release system; the cartilage organ chip is used for simulating the physiological and pathological states of articular cartilage; the mechanical stress loading system simulates joint movement through mechanical stress; the inflammatory factor release system is used for controlling the release of inflammatory factors and simulating the inflammatory environment of arthritis.
[0009] As a further improvement of the present invention, the internal part of the cartilage organ chip contains a microstructure simulating cartilage tissue; a cell culture interface is arranged at the upper end of the cartilage organ chip.
[0010] As a further improvement of the present invention, the inflammatory factor release system includes a plurality of microfluidic channels and a liquid delivery system;
[0011] The microfluidic channels are arranged in the cartilage organ chip and are used for controlling the release of inflammatory factors; the liquid delivery system is used for delivering inflammatory factors to the microfluidic channels in the cartilage organ chip.
[0012] As a further improvement of the present invention, the mechanical stress loading system includes: a multi-degree-of-freedom loading head, a control mechanism and a data processing module;
[0013] The multi-degree-of-freedom loading head is used for providing periodic mechanical force stimulation to the cartilage organ chip;
[0014] The control mechanism includes a motor and a driver. The motor is used to provide power to the multi-degree-of-freedom loading head; the driver is used to receive and execute the instructions of the data processing module;
[0015] The data processing module is used to control the movement of the motor through the driver, thereby controlling the amplitude, direction, magnitude, and frequency of the stress load applied by the multi-degree-of-freedom loading head to the cartilage organ chip;
[0016] The data processing module includes a driver, a controller, and a motor; the driver is connected to the controller and is used to receive and execute the instructions sent by the controller; the controller is connected to the motor and is used to control the movement of the motor; the motor is used to drive the multi-degree-of-freedom loading head to provide periodic mechanical force stimulation to the cartilage organ chip.
[0017] On this basis, the present invention also provides a method for using an arthritis stem cell therapy drug screening device, which is implemented based on the above screening device; this method includes the following steps:
[0018] The cell culture module amplifies, differentiates, and maintains the physiological state of stem cells; the organ chip simulation module simulates the pathological environment of knee osteoarthritis and evaluates the effects of drugs on chondrocytes; the data processing module collects and analyzes the experimental data in the organ chip simulation module and evaluates the toxic effects and regulatory effects of the drugs to be screened on stem cells.
[0019] On this basis, the present invention also provides an arthritis stem cell therapy drug screening method, which is implemented using the above screening device;
[0020] This method includes the following steps:
[0021] S100: Determine the drug action target according to the arthritis disease mechanism and select a drug candidate list;
[0022] S200: Design drug screening conditions according to the drug action target; based on the two parameters of drug dose and action time, generate a drug screening matrix according to the drug screening conditions;
[0023] S300: According to the drug screening matrix, apply combined stimulation to stem cells through the mechanical stress loading system and the inflammatory factor release system, and use the cartilage organ chip in the organ chip simulation module to evaluate the effects of the drugs to be screened on chondrocytes;
[0024] S400: Co-culture the drug to be screened with stem cells for a period of time, and evaluate the toxic effects of the drug to be screened on stem cells;
[0025] S500: Based on the physiological and phenotypic data of stem cells collected and analyzed by the data processing module, evaluate the regulatory effect of the drug to be screened on the chondrocyte phenotype, so as to evaluate the osteogenic and chondrogenic effects of the drug according to the evaluation results.
[0026] As a further improvement of the present invention, the mechanical stress loading system simulates the movement load of joints, and analyzes the regulatory effect of the drug to be screened under cyclic load and the osteogenic and chondrogenic functions of stem cells by repeatedly switching between applying force and not applying force periodically.
[0027] As a further improvement of the present invention, the inflammatory factor release system analyzes the regulatory effect of inflammatory factors on the stem cell phenotype by simulating the inflammatory environment of arthritis, so as to evaluate the effectiveness of the drug to be screened for the treatment of arthritis.
[0028] As a further improvement of the present invention, the organ chip simulation module includes a cartilage organ chip, a mechanical stress loading system and an inflammatory factor release system;
[0029] The mechanical stress loading system includes a multi-degree-of-freedom loading head, a motor and a controller; the multi-degree-of-freedom loading head provides periodic mechanical load stimulation to the cartilage organ chip; the mechanical stress loading system simulates the movement load of joints through mechanical stress, determines the drug action target according to the arthritis disease mechanism, and is used to simulate joint movement; the inflammatory factor release system is used to control the release of inflammatory factors and simulate the inflammatory environment of arthritis.
[0030] As a further improvement of the present invention, the organ chip simulation module simulates the pathological environment of arthritis by controlling the concentration of the drug solution to be screened, the applied force and the release of inflammatory factors.
[0031] The method for controlling the multi-degree-of-freedom loading head to apply stress to the cartilage organ chip includes the following steps:
[0032] The data processing module sends a signal to the driver, the driver controls the controller, the controller controls the motor, and further controls the multi-degree-of-freedom loading head to apply periodic mechanical load to the cartilage organ chip.
[0033] The above improved technical features can be combined with each other as long as they do not conflict with each other.
[0034] Generally speaking, compared with the prior art, the beneficial effects of the above technical solutions conceived by the present invention include:
[0035] (1) The arthritis stem cell therapy drug screening device, its usage, and screening method of the present invention amplify, differentiate stem cells, and maintain the physiological state of stem cells, directly add the drug to be screened into the stem cell culture system, simulate the pathological environment of arthritis, evaluate the impact of the drug on chondrocytes, collect and analyze experimental data to confirm and optimize the best drug combination, reduce the number of animal models required for traditional tests, improve the drug screening efficiency, reduce costs, and accelerate the drug R & D process.
[0036] (2) The arthritis stem cell therapy drug screening device, its usage, and screening method of the present invention apply periodic loads to chondrocytes through a mechanical stress loading system, which helps to improve the culture conditions of stem cells, simulate the mechanical load of arthritis patients, and more accurately evaluate the therapeutic effect of the drug on chondrocytes; at the same time, through the inflammatory factor release system, inflammatory factors can be precisely released to simulate the inflammatory environment of arthritis, promote the self - repair and regeneration ability of stem cells, help the proliferation of chondrocytes, and repair damaged joint tissues. Brief Description of the Drawings
[0037] Figure 1 is a schematic diagram of the overall composition of the arthritis stem cell therapy drug screening device in the embodiment of the present invention; Detailed Embodiments
[0038] In order to make the objectives, technical solutions, and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the drawings and embodiments.
[0039] It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention. In addition, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.
[0040] Please refer to Figure 1, the present invention provides a screening device for arthritis stem cell therapy drugs, which includes a cell culture module, a drug treatment module, an organ chip simulation module, and a data analysis module; the cell culture module is used for amplifying, differentiating, and maintaining the physiological state of stem cells; the drug treatment module is used to accurately add the drugs to be screened into the stem cell culture system and control the concentration and action time of the drugs; the organ chip simulation module is used to simulate the pathological environment of arthritis and evaluate the impact of drugs on chondrocytes; the data analysis module is used to collect and analyze the experimental data in the organ chip simulation module and evaluate the potential therapeutic effect of the drugs. The organ chip simulation module includes a cartilage organ chip, a mechanical stress loading system, and an inflammatory factor release system; the cartilage organ chip is used to simulate the physiological and pathological states of articular cartilage; the mechanical stress loading system simulates joint movement through mechanical stress; the inflammatory factor release system is used to control the release of inflammatory factors and simulate the inflammatory environment of arthritis.
[0041] During the actual use process, the cell culture module is responsible for culturing and amplifying the stem cells to be screened. It generally includes a sterile incubator, cell culture dishes, and a culture medium delivery system. The cell culture dishes are placed inside the sterile incubator, and the culture medium delivery system is connected to the cell culture dishes through pipelines to achieve automatic replacement and supplementation of the culture medium. The above structural features are all relatively common devices in the art, and their specific usage methods will not be elaborated here too much. Those skilled in the art can easily master them.
[0042] More specifically, the inside of the cartilage organ chip contains a microstructure that simulates cartilage tissue; the upper end of the cartilage organ chip is provided with a cell culture interface. The inflammatory factor release system includes multiple microfluidic channels and a liquid delivery system; the microfluidic channels are arranged in the cartilage organ chip and are used to control the release of inflammatory factors; the liquid delivery system is used to deliver inflammatory factors to the microfluidic channels in the cartilage organ chip.
[0043] It should be noted that the organ chip simulation module simulates the pathological environment of knee osteoarthritis and evaluates the impact of drugs on chondrocytes. The cartilage organ chip is connected to the mechanical stress loading system to simulate the mechanical stress during joint movement; the inflammatory factor release system is connected to the cartilage organ chip to simulate the inflammatory environment of arthritis. At the same time, the cartilage organ chip is connected to the cell culture module to receive the stem cells that have been treated with drugs.
[0044] Furthermore, the mechanical stress loading system in the preferred embodiment of the present invention includes a multi-degree-of-freedom loading head, a control mechanism, and a data processing module; the multi-degree-of-freedom loading head is used to provide periodic mechanical force stimulation to the cartilage organ chip; the control mechanism includes a motor and a driver, the motor is used to provide power to the multi-degree-of-freedom loading head; the driver is used to receive and execute the instructions of the data processing module; the data processing module is used to control the movement of the motor through the driver, so as to control the amplitude, direction, magnitude, and frequency of the stress load applied by the multi-degree-of-freedom loading head to the cartilage organ chip.
[0045] More specifically, in the preferred embodiment of the present invention, the data processing module includes a driver, a controller, and a motor; the driver is connected to the controller and is used to receive and execute the instructions sent by the controller; the controller is connected to the motor and is used to control the movement of the motor; the motor is used to drive the multi-degree-of-freedom loading head to provide periodic mechanical force stimulation to the cartilage organ chip.
[0046] In addition, the data analysis module is used to collect and analyze the experimental data in the organ chip simulation module and evaluate the potential therapeutic effect of the drug. It includes a microscope, an image acquisition system, and data analysis software, etc. The microscope is connected to the image acquisition system and is used to observe the morphological changes of chondrocytes; the image acquisition system is connected to the data analysis software to realize the automated processing and analysis of experimental data.
[0047] More specifically, during actual use, the operator first uses a microscope to observe the morphology of chondrocytes in the cartilage organ chip. The microscope should have high resolution so as to clearly capture the morphological changes of cells, such as cell proliferation, apoptosis, morphological abnormalities, etc. Since the microscope is connected to the image acquisition system, it can convert the observed cell morphology into digital images. During image acquisition, relevant experimental parameters are recorded, such as drug concentration, action time, mechanical stress loading conditions, etc. The acquired images are preprocessed, including denoising, contrast enhancement, image segmentation, etc. Cell features are extracted from the preprocessed images, such as cell number, morphological parameters, expression levels of cell proliferation markers, etc. The extracted cell features are combined with the experimental parameters to form a complete experimental data set. The data set should include data from multiple experimental groups and control groups for subsequent comparison and analysis. Statistical methods are used to process the experimental data, such as mean, standard deviation, t-test, analysis of variance, etc. According to the data analysis results, the potential therapeutic effect of the drug is evaluated. The evaluation indicators include cell proliferation rate, apoptosis rate, degree of morphological change, etc.
[0048] Generally speaking, the present invention provides a stable and controllable growth environment for stem cells through a sterile incubator and a cell culture dish. At the same time, with the introduction of the culture medium delivery system, automatic replacement and supplementation of the culture medium are realized, improving the efficiency and stability of cell culture. Meanwhile, through the precision pump and drug delivery pipeline in the drug treatment module, precise addition and concentration control of drugs are achieved. This helps to accurately evaluate the impact of drugs on stem cells and reduce experimental errors. And innovatively, the cartilage organ chip is combined with the mechanical stress loading system and the inflammatory factor release system to simulate the pathological environment of knee osteoarthritis. This not only improves the accuracy and reliability of drug screening, but also provides a new platform for in-depth research on the pathogenesis and treatment methods of arthritis. Finally, the data analysis module realizes the automatic processing and analysis of experimental data through the integration of a microscope, an image acquisition system, and data analysis software. This greatly improves the efficiency and accuracy of data analysis, helping to quickly screen out drugs with potential therapeutic effects.
[0049] On this basis, the present invention also provides a method for using an apparatus for screening drugs for treating arthritis with stem cells, which is implemented based on the above-mentioned screening apparatus; the method includes the following steps:
[0050] The cell culture module amplifies and differentiates stem cells and maintains the physiological state of stem cells; the organ chip simulation module simulates the pathological environment of knee osteoarthritis and evaluates the impact of drugs on chondrocytes; the data processing module collects and analyzes the experimental data in the organ chip simulation module and evaluates the toxic effects and regulatory effects of the drugs to be screened on stem cells.
[0051] On this basis, the present invention also provides a method for screening drugs for treating arthritis with stem cells, which is implemented using the screening apparatus;
[0052] The method includes the following steps:
[0053] S100: Determine the drug action target according to the pathogenesis of arthritis and select a list of drug candidates;
[0054] S200: Design drug screening conditions according to the drug action target; based on the two parameters of drug dose and action time, generate a drug screening matrix according to the drug screening conditions;
[0055] S300: According to the drug screening matrix, apply a combined stimulus to stem cells through the mechanical stress loading system and the inflammatory factor release system, and use the cartilage organ chip in the organ chip simulation module to evaluate the impact of the drugs to be screened on chondrocytes;
[0056] S400: Co-culture the drugs to be screened with stem cells for a period of time, and evaluate the toxic effects of the drugs to be screened on stem cells;
[0057] S500: Based on the physiological and phenotypic data of stem cells collected and analyzed by the data processing module, evaluate the regulatory effect of the drug to be screened on the chondrocyte phenotype, and thus evaluate the osteogenic and chondrogenic effects of the drug according to the evaluation results.
[0058] The mechanical stress loading system simulates the movement load of joints, and analyzes the regulatory effect of the drug to be screened under cyclic load and the osteogenic and chondrogenic functions of stem cells by repeatedly switching between the periodic application of force and the non-application of force.
[0059] The inflammatory factor release system analyzes the regulatory effect of inflammatory factors on the stem cell phenotype by simulating the inflammatory environment of arthritis, and thus evaluates the effectiveness of the drug to be screened for the treatment of arthritis.
[0060] The organ-on-a-chip simulation module includes a cartilage organ-on-a-chip, a mechanical stress loading system, and an inflammatory factor release system;
[0061] The mechanical stress loading system includes a multi-degree-of-freedom loading head, a motor, and a controller; the multi-degree-of-freedom loading head provides periodic mechanical load stimulation to the cartilage organ-on-a-chip; the mechanical stress loading system simulates the movement load of joints through mechanical stress, determines the drug action target according to the arthritis disease mechanism, and is used to simulate joint movement; the inflammatory factor release system is used to control the release of inflammatory factors and simulate the inflammatory environment of arthritis.
[0062] The organ-on-a-chip simulation module simulates the pathological environment of arthritis by controlling the concentration of the drug solution to be screened, the applied force, and the release of inflammatory factors.
[0063] The method for controlling the multi-degree-of-freedom loading head to apply stress to the cartilage organ-on-a-chip includes the following steps:
[0064] The data processing module sends a signal to the driver, the driver controls the controller, the controller controls the motor, and further controls the multi-degree-of-freedom loading head to apply periodic mechanical load to the cartilage organ-on-a-chip.
[0065] (1) The arthritis stem cell therapy drug screening device, its use, and screening method of the present invention amplify, differentiate stem cells and maintain the physiological state of stem cells, directly add the drug to be screened into the stem cell culture system, simulate the pathological environment of arthritis, evaluate the effect of the drug on chondrocytes, collect and analyze experimental data to confirm and optimize the best drug combination, reduce the number of animal models required for traditional tests, improve the drug screening efficiency, reduce costs, and accelerate the drug R & D process.
[0066] (2) The arthritis stem cell therapy drug screening device, its use and screening method of the present invention apply periodic loads to chondrocytes through a mechanical stress loading system, which helps to improve the culture conditions of stem cells, simulate the mechanical load of arthritis patients, and more accurately evaluate the therapeutic effect of drugs on chondrocytes; at the same time, through the inflammatory factor release system, inflammatory factors can be precisely released, simulating the inflammatory environment of arthritis, promoting the self-repair and regeneration ability of stem cells, helping the proliferation of chondrocytes, and repairing damaged joint tissues.
[0067] Those skilled in the art can easily understand that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent replacements, and improvements made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.
Claims
1. An apparatus for screening drugs for treating arthritis with stem cells, characterized in that, It includes a cell culture module, a drug treatment module, an organ chip simulation module, and a data analysis module; The cell culture module is used for amplifying, differentiating stem cells, and maintaining the physiological state of stem cells; the drug treatment module is used for precisely adding the drug to be screened into the stem cell culture system and controlling the concentration and action time of the drug; the organ chip simulation module is used for simulating the pathological environment of arthritis and evaluating the effect of the drug on chondrocytes; the data analysis module is used for collecting and analyzing the experimental data in the organ chip simulation module and evaluating the potential therapeutic effect of the drug; The organ chip simulation module includes a cartilage organ chip, a mechanical stress loading system, and an inflammatory factor release system; the cartilage organ chip is used for simulating the physiological and pathological states of articular cartilage; the mechanical stress loading system simulates joint movement through mechanical stress; the inflammatory factor release system is used for controlling the release of inflammatory factors and simulating the inflammatory environment of arthritis.
2. The screening device for arthritis stem cell therapy drugs according to claim 1, characterized in that, The interior of the cartilage organ chip contains a microstructure simulating cartilage tissue; a cell culture interface is arranged at the upper end of the cartilage organ chip.
3. The arthritis stem cell therapy drug screening device according to claim 1, wherein The inflammatory factor release system includes a plurality of microfluidic channels and a liquid delivery system; The microfluidic channels are arranged in the cartilage organ chip and are used for controlling the release of inflammatory factors; the liquid delivery system is used for delivering inflammatory factors to the microfluidic channels in the cartilage organ chip.
4. The arthritis stem cell therapy drug screening device according to any one of claims 1 to 3, characterized in that The mechanical stress loading system includes a multi-degree-of-freedom loading head, a control mechanism, and a data processing module; The multi-degree-of-freedom loading head is used for providing periodic mechanical force stimulation to the cartilage organ chip; The control mechanism includes a motor and a driver. The motor is used for providing power to the multi-degree-of-freedom loading head; the driver is used for receiving and executing the instructions of the data processing module; The data processing module is used for controlling the movement of the motor through the driver, thereby controlling the amplitude, direction, magnitude, and frequency of the stress load applied by the multi-degree-of-freedom loading head to the cartilage organ chip; The data processing module includes a driver, a controller, and a motor; the driver is connected to the controller and is used for receiving and executing the instructions sent by the controller; the controller is connected to the motor and is used for controlling the movement of the motor; the motor is used for driving the multi-degree-of-freedom loading head to provide periodic mechanical force stimulation to the cartilage organ chip.
5. A method for using a screening device for arthritis stem cell therapy drugs, characterized in that, It is implemented based on the device according to any one of claims 1 to 4; the method includes the following steps: The cell culture module amplifies, differentiates stem cells, and maintains the physiological state of stem cells; the organ chip simulation module simulates the pathological environment of knee osteoarthritis and evaluates the effect of the drug on chondrocytes; the data processing module collects and analyzes the experimental data in the organ chip simulation module and evaluates the toxic effect and regulatory effect of the drug to be screened on stem cells.
6. A screening method for stem cell drugs for treating arthritis, characterized in that, It is implemented by using the screening device according to any one of claims 1 to 4; The method includes the following steps: S100: Determine the drug action target according to the arthritis disease mechanism and select a list of drug candidates; S200: Design drug screening conditions according to the drug action target; according to the drug screening conditions, generate a drug screening matrix based on the two parameters of drug dose and action time; S300: According to the drug screening matrix, apply combined stimulation to the stem cells through the mechanical stress loading system and the inflammatory factor release system, and use the cartilage organ chip in the organ chip simulation module to evaluate the effect of the drug to be screened on chondrocytes; S400: Co-culture the drug to be screened with the stem cells for a period of time, and evaluate the toxic effect of the drug to be screened on the stem cells; S500: Based on the physiological and phenotypic data of the stem cells collected and analyzed by the data processing module, evaluate the regulatory effect of the drug to be screened on the chondrocyte phenotype, so as to evaluate the osteogenic and chondrogenic effects of the drug according to the evaluation results.
7. The arthritis stem cell therapy drug screening device according to claim 6, wherein, The mechanical stress loading system simulates the movement load of the joint, and analyzes the regulatory effect of the drug to be screened under cyclic load and the osteogenic and chondrogenic functions of the stem cells by repeatedly switching between periodically applying force and not applying force.
8. The arthritis stem cell therapy drug screening device according to claim 6, characterized in that, The inflammatory factor release system analyzes the regulatory effect of inflammatory factors on the stem cell phenotype by simulating the inflammatory environment of arthritis, so as to evaluate the effectiveness of the drug to be screened for the treatment of arthritis.
9. The screening device for arthritis stem cell therapy drugs according to claim 6, wherein The organ chip simulation module includes a cartilage organ chip, a mechanical stress loading system, and an inflammatory factor release system; The mechanical stress loading system includes a multi-degree-of-freedom loading head, a motor, and a controller; the multi-degree-of-freedom loading head provides periodic mechanical load stimulation to the cartilage organ chip; the mechanical stress loading system simulates the movement load of the joint through mechanical stress, determines the drug action target according to the arthritis disease mechanism, and is used to simulate joint movement; The inflammatory factor release system is used to control the release of inflammatory factors and simulate the inflammatory environment of arthritis.
10. The arthritis stem cell therapy drug screening device according to claim 9, characterized in that, The organ chip simulation module simulates the pathological environment of arthritis by controlling the concentration of the drug solution to be screened, the applied force, and the release of inflammatory factors. The method for controlling the multi-degree-of-freedom loading head to apply stress to the cartilage organ chip includes the following steps: The data processing module sends a signal to the driver, the driver controls the controller, the controller controls the motor, and further controls the multi-degree-of-freedom loading head to apply periodic mechanical load to the cartilage organ chip.