Blood cell counting device and detection method thereof

Abstract

The invention discloses a blood cell counting device and a detection method thereof. The blood cell counting device comprises a front tank, a rear tank and a counting hole, wherein the front tank is communicated with the rear tank through the counting hole; a pipetting assembly and a suction head; during pipetting, the pipetting assembly is connected with the suction head in an inserting manner, and a sample is moved to the forebay through the suction head, so that the forebay is loaded with a to-be-detected sample; when blood cell counting is carried out, the pipetting assembly is connected with the rear pool in an inserted mode and used for building pressure for the rear pool, and a to-be-detected sample in the front pool flows into the rear pool through the counting hole so that the to-be-detected sample can be detected. According to the mode, the pipetting assembly is inserted into the suction head to achieve the pipetting function, the pipetting assembly is inserted into the rear pool to achieve pressure buildup on the rear pool, namely, the blood cell counting device achieves pipetting and pressure buildup counting through the same pipetting assembly, two sets of liquid paths are not needed, the structure is simple, implementation is easy, and the cost is reduced.

Description

Technical Field

[0001] This application relates to the technical field of blood cell detection, and particularly to a blood cell counting device and a detection method thereof. Background Art

[0002] The blood cell counting device detects a sample to be measured by the impedance method. The blood cell counting device includes a front chamber, a rear chamber, a gem hole, a liquid transfer assembly, and a negative pressure assembly. The gem hole is used to connect the front chamber and the rear chamber. The liquid transfer assembly is used to transfer the sample to be measured to the front chamber, and the negative pressure assembly is used to build pressure in the rear chamber so that the pressure in the rear chamber reaches the target pressure value. The sample to be measured in the front chamber flows into the rear chamber through the gem hole to realize the detection of the sample to be measured.

[0003] Among them, since the liquid transfer assembly and the negative pressure assembly of the blood cell counting device are independent of each other, the liquid transfer assembly and the negative pressure assembly require two sets of liquid paths, and the structure is complex, resulting in high costs. Summary of the Invention

[0004] This application provides a blood cell counting device and a detection method thereof to solve the technical problems that occur in the prior art.

[0005] To solve the above problems, a first aspect of this application provides a blood cell counting device, including:

[0006] A front chamber, a rear chamber, and a counting hole, the front chamber is connected to the rear chamber through the counting hole;

[0007] A liquid transfer assembly and a pipette tip;

[0008] When performing liquid transfer, the liquid transfer assembly inserts the pipette tip and transfers the sample to the front chamber through the pipette tip so that the front chamber is loaded with the sample to be measured;

[0009] When performing blood cell counting, the liquid transfer assembly inserts into the rear chamber and is used to build pressure in the rear chamber. The sample to be measured in the front chamber flows into the rear chamber through the counting hole to detect the sample to be measured.

[0010] Among them, the liquid transfer assembly includes a syringe assembly and a pipettor. The syringe assembly is connected to the first end of the pipettor, and the second end of the pipettor inserts the pipette tip or the rear chamber; when performing blood cell counting, the connection end of the second end of the pipettor and the rear chamber is in interference fit.

[0011] Among them, the rear chamber includes a rear chamber housing and a rear chamber electrode. When performing blood cell counting, the liquid transfer assembly inserts into the rear chamber housing to form a rear chamber cavity. One end of the rear chamber electrode is disposed in the rear chamber cavity, and the other end of the rear chamber electrode is disposed outside the rear chamber cavity.

[0012] Among them, the blood cell counting device is used to detect a first detection item of the sample to be tested, and the volume of the rear pool cavity is greater than the sum of the volume of the first detection item and a preset remaining volume;

[0013] Alternatively, the blood cell counting device is used to detect a second detection item of the sample to be tested, and the volume of the rear pool cavity is greater than the sum of the volume of the second detection item and the preset remaining volume;

[0014] Alternatively, the blood cell counting device is used to detect the first detection item and the second detection item of the sample to be tested, and the volume of the rear pool cavity is greater than the sum of the volume of the first detection item, the volume of the second detection item, and the preset remaining volume.

[0015] Among them, the blood cell counting device further includes a microplate, the counting holes are arranged on the microplate, the rear pool housing includes a through hole, the microplate is correspondingly arranged with the through hole, and the microplate is detachably connected or integrally formed with the rear pool housing.

[0016] Among them, the blood cell counting device further includes a first electrode, and the other end of the rear pool electrode is inserted into the first electrode.

[0017] Among them, the first electrode includes an elastic sheet, the elastic sheet has a plug-in portion, the cross-sectional area of the plug-in portion is smaller than the cross-sectional area of the rear pool electrode, and the other end of the rear pool electrode is inserted into the plug-in portion.

[0018] Among them, the blood cell counting device further includes a blood cell counting instrument and a reagent kit. The blood cell counting instrument includes the pipetting assembly. The reagent kit includes a front pool and a front pool electrode. One end of the front pool electrode is located inside the front pool, and the other end of the front pool electrode is located outside the front pool; the reagent kit and the rear pool are loaded in the blood cell counting instrument to detect the sample to be tested.

[0019] Among them, the reagent kit includes an accessory placement area for placing the rear pool;

[0020] When the blood cell counting is completed, the pipetting assembly withdraws the rear pool to the accessory placement area.

[0021] To solve the above problems, a second aspect of the present application provides a detection method applied to the above blood cell counting device. The detection method includes:

[0022] Insert the pipetting assembly with a pipette tip, and transfer the sample to the front pool through the pipette tip;

[0023] Dilute and / or mix the sample through the pipetting assembly and the pipette tip to obtain a sample to be tested, and withdraw the pipette tip through the pipetting assembly;

[0024] Insert the pipetting assembly into the front cell, and connect the front cell to the rear cell through the counting hole to detect the sample to be tested.

[0025] The beneficial effect of this application is that: different from the prior art, the blood cell counting device of this application includes: a front cell, a rear cell and a counting hole, and the front cell is connected to the rear cell through the counting hole; a pipetting assembly and a pipette tip; when pipetting, the pipetting assembly inserts the pipette tip, and the sample is pipetted into the front cell through the pipette tip so that the front cell is loaded with the sample to be tested; when performing blood cell counting, the pipetting assembly inserts into the rear cell to build pressure on the rear cell, and the sample to be tested in the front cell flows into the rear cell through the counting hole to detect the sample to be tested. In the above manner, the pipetting assembly inserts the pipette tip to achieve the pipetting function, and the pipetting assembly inserts into the rear cell to achieve pressure building on the rear cell, that is, the blood cell counting device realizes pipetting and pressure building counting through the same pipetting assembly, without two sets of liquid paths, with a simple structure, easy to implement, and cost reduction. Description of the Drawings

[0026] In order to more clearly illustrate the technical solutions in the embodiments of this application, the following will briefly introduce the drawings required for description in the embodiments. Obviously, the following drawings are only some embodiments of this application. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings. Among them:

[0027] Figure 1 is a schematic framework diagram of an embodiment of the blood cell counting device of this application;

[0028] Figure 2 is a schematic structural diagram of another embodiment of the blood cell counting device of this application;

[0029] Figure 3 is Figure 2 a schematic structural diagram of an embodiment of the pipetting assembly inserting the pipette tip in ;

[0030] Figure 4 is Figure 2 a schematic structural diagram of an embodiment of the rear cell, the pipetting assembly and the microplate in ;

[0031] Figure 5 is Figure 2 a schematic structural diagram of an embodiment of the first electrode in ;

[0032] Figure 6 is Figure 2 a schematic structural diagram of an embodiment of the pipetting assembly in ;

[0033] Figure 7 is Figure 2 a schematic structural diagram of an embodiment of a pipettor;

[0034] Figure 8 is Figure 2 a schematic structural diagram of another embodiment of a liquid transfer assembly;

[0035] Figure 9 is a schematic flowchart of an embodiment of the detection method of the present application;

[0036] Figure 10 is a schematic framework diagram of an embodiment of a computer-readable storage medium of the present application. Detailed implementation manners

[0037] Next, the technical solutions in the embodiments of the present application will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present application.

[0038] The terms "first", "second", "third", "fourth", etc. (if any) in the specification, claims and above-mentioned drawings of the present application are used to distinguish similar objects, and do not necessarily need to be used to describe a specific order or sequence. It should be understood that the data used in this way can be interchanged under appropriate circumstances, so that the embodiments of the present application described here, for example, can be implemented in an order other than those illustrated or described here. In addition, the terms "comprising" and "having" and any variations thereof are intended to cover non-exclusive inclusion. For example, a process, method, system, product or device comprising a series of steps or units does not necessarily have to be limited to those steps or units clearly listed, but may include other steps or units not clearly listed or inherent to these processes, methods, products or devices.

[0039] Please refer to Figure 1 as shown Figure 1 is a schematic framework diagram of an embodiment of a blood cell counting device of the present application. The blood cell counting device 10 of the present application may include a POCT (point-of-care testing) blood cell analyzer or a hematology analyzer.

[0040] In this embodiment, the blood cell counting device 10 is taken as a POCT blood cell analyzer as an example for illustration. The blood cell counting device 10 of this embodiment includes a reagent kit 11, a negative pressure assembly 12, a detection assembly 13, and a liquid transfer assembly 14.

[0041] The kit 11 includes a cartridge 111 and a microplate 110. The cartridge 111 includes a front chamber 112 and a rear chamber 113. The rear chamber 113 is located on one side of the front chamber 112. A through hole (not shown in the figure) is provided between the front chamber 112 and the rear chamber 113. The microplate 110 is disposed at one end of the through hole. For example, the microplate 110 is located at one end of the through hole close to the front chamber 112, or the microplate 110 is located at one end of the through hole close to the rear chamber 113.

[0042] Optionally, the kit 11 further includes a front chamber electrode 114 and a rear chamber electrode 115. One end of the front chamber electrode 114 is located inside the front chamber 112, and the other end of the front chamber electrode 114 is located outside the cartridge 111. One end of the rear chamber electrode 115 is located inside the rear chamber 113, and the other end of the rear chamber electrode 115 is located outside the cartridge 111.

[0043] For example, the front chamber electrode 114 and the rear chamber electrode 115 are located on the same side of the cartridge 111, which is convenient for abutting against the detection assembly 13. In other embodiments, the front chamber electrode 114 and the rear chamber electrode 115 may be located on different sides of the cartridge 111 respectively, which will not be elaborated here.

[0044] The pipetting assembly 14 is used for pipetting to add a sample to be tested into the front chamber 112. The negative pressure assembly 12 is connected to the rear chamber 113 and is used to establish a negative pressure in the rear chamber 113. The rear chamber 113 is provided with a negative pressure interface, and the negative pressure assembly 12 is connected to the negative pressure interface; the negative pressure interface, the front chamber electrode 114 and the rear chamber electrode 115 are located on the same side of the cartridge 111. In other embodiments, the negative pressure interface, the front chamber electrode 114 and the rear chamber electrode 115 may be located on different sides of the cartridge 111 respectively.

[0045] The detection assembly 13 is respectively connected to the front chamber electrode 114 and the rear chamber electrode 115; when the sample to be tested in the front chamber 112 enters the rear chamber 113 through the micropores of the microplate 110, the detection assembly 13 is used to collect signals and process the signals to realize the detection of the sample to be tested located in the kit 11.

[0046] Since the pipetting assembly 14 of the blood cell counting device 10 is used for pipetting and the negative pressure assembly 12 is used to establish a negative pressure in the rear chamber 113; therefore, the pipetting assembly 14 and the negative pressure assembly 12 are independent of each other, and the pipetting assembly 14 and the negative pressure assembly 12 require two sets of liquid paths (i.e., the liquid path of the pipetting assembly 14 and the liquid path of the negative pressure assembly 12), with a complex structure, resulting in high costs.

[0047] To solve Figure 1 the technical problem that the pipetting assembly 14 and the negative pressure assembly 12 are independent of each other, resulting in a complex structure and high costs, the present application also provides a blood cell counting device 20. Please refer to

[0048] Figure 2-3 as shown in Figure 2It is a schematic structural diagram of another embodiment of the blood cell counting device of the present application;

[0049] Figure 3 is Figure 2 a schematic structural diagram of an embodiment of the pipetting assembly inserting a pipette tip in

[0050] The blood cell counting device 20 of this embodiment includes a front chamber 211, a rear chamber 22, a counting hole (not shown in the figure), a pipetting assembly 23, and a pipette tip 24.

[0051] Among them, the front chamber 211 and the rear chamber 22 are connected through the counting hole, and the counting hole may include Figure 1 the micropores of the microporous plate 110 in . When the blood cell counting device 20 performs blood cell counting, the front chamber 211 is used to load the sample to be tested, and the counting hole is used to allow the blood cells in the sample to be tested located in the front chamber 211 to pass through one by one. The diameter of the counting hole can be correspondingly set according to the different particle sizes of the blood cells.

[0052] Optionally, the blood cell counting device 20 further includes a reagent kit 21, and the front chamber 211 is arranged on the reagent kit 21. Compared with Figure 1 the reagent kit 11 in , the reagent kit 21 of this embodiment is provided with a front chamber 211 and does not have a rear chamber 22. Therefore, the structure of the reagent kit 21 is simple and easy to implement.

[0053] In other embodiments, the rear chamber 22 of this embodiment can also be arranged on the reagent kit 21, which will not be elaborated here.

[0054] Optionally, the reagent kit 21 may further include an accessory placement area, and the pipette tip 24 is placed in the accessory placement area. The pipette tip 24 belongs to disposable consumables. That is, after the blood cell counting device 20 completes the detection of the sample to be tested, a new pipette tip 24 needs to be replaced to avoid contaminating the sample to be tested.

[0055] When the blood cell counting device 20 performs pipetting, the pipetting assembly 23 inserts the pipette tip 24, and transfers the sample to the front chamber 211 through the pipette tip 24, so that the front chamber 211 is loaded with the sample to be tested. The pipetting assembly 23 inserting the pipette tip 24 means that the pipetting assembly 23 inserts one end of the pipette tip 24, so that the pipetting assembly 23 is connected to one end of the pipette tip 24, and the liquid is sucked through the other end of the pipette tip 24. The liquid includes but is not limited to the diluent.

[0056] For example, the kit 21 further includes a sample placement area (not shown in the figure) and a diluent placement area (not shown in the figure). The sample placement area is used to place the sample, and the diluent placement area is used to place the diluent. The pipetting assembly 23 inserts the pipette tip 24, and transfers the sample in the sample placement area to the front chamber 211 through the pipette tip 24. Then, the diluent in the diluent placement area is transferred to the front chamber 211 through the pipette tip 24 to perform a dilution operation on the sample in the front chamber 211, obtaining the sample to be tested, that is, the front chamber 211 is loaded with the sample to be tested. In other embodiments, the sample can be placed in the front chamber 211, the pipetting assembly 23 inserts the pipette tip 24, and the diluent in the diluent placement area is transferred to the front chamber 211 through the pipette tip 24 to perform a dilution operation on the sample in the front chamber 211.

[0057] When the blood cell counting device 20 performs blood cell counting, the pipetting assembly 23 inserts into the rear chamber 22 to build pressure in the rear chamber 22. The sample to be tested in the front chamber 211 flows into the rear chamber 22 through the counting hole to test the sample to be tested.

[0058] For example, the rear chamber 22 has a negative pressure interface 221. The pipetting assembly 23 inserts and connects to the negative pressure interface 221. The pipetting assembly 23 is used to build pressure in the rear chamber 22 so that the pressure in the rear chamber 22 reaches the target pressure range. At this time, the sample to be tested in the front chamber 211 flows into the rear chamber 22 through the counting hole to test the sample to be tested.

[0059] When the pipetting assembly 23 of this embodiment performs pipetting, it inserts the pipette tip 24 to perform pipetting and realizes the pipetting function; when the pipetting assembly 23 performs blood cell counting, it inserts into the rear chamber 22 to build pressure in the rear chamber 22. That is, the blood cell counting device 20 realizes pipetting and pressure building for counting through the same pipetting assembly 23, without two sets of liquid paths, with a simple structure, easy to implement, and reduced costs.

[0060] Please refer to Figure 2-4 shown in Figure 4 is Figure 2 a schematic structural diagram of an embodiment of the rear chamber, the pipetting assembly, and the microplate in the figure. The rear chamber 22 of this embodiment includes a rear chamber housing 222 and a rear chamber electrode 223. The rear chamber housing 222 has a negative pressure interface 221.

[0061] Among them, the rear chamber electrode 223 can be integrally molded with the rear chamber housing 222 through an in-mold injection process, which can improve the sealing performance between the rear chamber electrode 223 and the rear chamber housing 222. In other embodiments, the rear chamber electrode 223 is detachably connected to the rear chamber housing 222.

[0062] When the blood cell counting device 20 performs blood cell counting, the pipetting assembly 23 inserts into the rear chamber housing 222, that is, the pipetting assembly 23 inserts into the negative pressure interface 221. The pipetting assembly 23 and the rear chamber housing 222 form a rear chamber cavity, and the rear chamber cavity is used to place the sample to be tested (i.e., waste liquid) flowing through the counting hole.

[0063] One end of the rear cell electrode 223 is disposed inside the rear cell cavity, and the other end of the rear cell electrode 223 is disposed outside the rear cell cavity. That is, the rear cell electrode 223 is fixed to the rear cell housing 222. One end of the rear cell electrode 223 is located inside the rear cell housing 222, and the other end of the rear cell electrode 223 is located outside the rear cell housing 222.

[0064] Compared with Figure 1 the negative pressure assembly 12 in [reference], the pipetting assembly 23 of this embodiment is inserted into the rear cell housing 222. The pipetting assembly 23 and the rear cell housing 222 form the rear cell cavity. The pipetting assembly 23 builds pressure in the rear cell 22, eliminating the need to set up the negative pressure assembly 12 and reducing costs.

[0065] According to some embodiments of the present application, the blood cell counting device 20 can be used to perform a first test item and / or a second test item on a sample to be tested. The first test item includes, but is not limited to, an RBC (Red Blood Cell) test item and / or a PLT (Platelet count) test item; the second test item includes, but is not limited to, a WBC (white blood cell) test item.

[0066] For example, when the blood cell counting device 20 performs an RBC test item on a sample to be tested, during pipetting, the pipetting assembly 23 inserts the pipette tip 24, and the diluent is transferred to the front cell 211 through the pipette tip 24 for dilution operation to obtain a sample to be tested with a first dilution ratio; when the blood cell counting device 20 performs a WBC test item on a sample to be tested, during pipetting, the pipetting assembly 23 transfers the diluent to the front cell 211 through the pipette tip 24 for dilution operation to obtain a sample to be tested with a second dilution ratio.

[0067] Optionally, the blood cell counting device 20 is used to perform a first test item on a sample to be tested, and the volume of the rear cell cavity is greater than the sum of the volume of the first test item and a preset remaining volume. Here, the volume of the first test item refers to the volume of the sample to be tested required for the blood cell counting device 20 to perform the first test item on the sample to be tested; the preset remaining volume refers to the safe volume after placing the waste liquid in the rear cell cavity to prevent the pipetting assembly 23 from being contaminated by the waste liquid.

[0068] For example, if the first test item is an RBC test item, then the volume of the rear cell cavity is greater than the sum of the volume of the RBC test item and the preset remaining volume; where the volume of the RBC test item is 180 μl and the preset remaining volume is 100 μl, then the volume of the rear cell cavity is greater than 280 μl.

[0069] Optionally, the blood cell counting device 20 is used to detect a second test item for the sample to be tested, and the volume of the rear pool cavity is greater than the sum of the volume of the second test item and a preset remaining volume. Wherein, the volume of the second test item refers to the volume of the sample to be tested required for the blood cell counting device 20 to detect the second test item for the sample to be tested.

[0070] For example, if the second test item is the WBC test item, then the volume of the rear pool cavity is greater than the sum of the volume of the WBC test item and the preset remaining volume; wherein, the volume of the WBC test item is 450 μl, and the preset remaining volume is 100 μl, then the volume of the rear pool cavity is greater than 550 μl.

[0071] Optionally, the blood cell counting device 20 is used to detect a first test item and a second test item for the sample to be tested, and the volume of the rear pool cavity is greater than the sum of the volume of the first test item, the volume of the second test item and the preset remaining volume.

[0072] For example, if the first test item is the RBC test item and the second test item is the WBC test item, then the volume of the rear pool cavity is greater than the sum of the volume of the RBC test item, the volume of the WBC test item and the preset remaining volume; wherein, the volume of the RBC test item is 180 μl, the volume of the WBC test item is 450 μl, and the preset remaining volume is 100 μl, then the volume of the rear pool cavity is greater than 730 μl.

[0073] Optionally, when the blood cell counting device 20 performs blood cell counting, the pipetting assembly 23 and the rear pool 22 are arranged in sequence along the gravity direction, that is, the pipetting assembly 23 is located above the rear pool 22. When the blood cell counting device 20 completes blood cell counting, the pipetting assembly 23 withdraws the rear pool 22 from the pipetting assembly 23, further avoiding the pipetting assembly 23 being contaminated by the waste liquid in the rear pool 22.

[0074] The volume of the rear pool cavity in this embodiment is greater than the sum of the volume of the first test item and / or the volume of the second test item and the preset remaining volume, which can ensure that the pipetting assembly 23 contacts the waste liquid in the rear pool cavity and avoid the pipetting assembly 23 being contaminated by the waste liquid in the rear pool cavity.

[0075] Please refer to Figure 2-4 As shown, the blood cell counting device 20 further includes a microplate 25, and counting holes are arranged on the microplate 25. The counting holes can also be referred to as gem holes or micro holes, etc.

[0076] The rear pool housing 222 includes a through hole 224, and the microplate 25 is correspondingly arranged with the through hole 224 so that the counting holes of the microplate 25 communicate with the through hole 224.

[0077] For example, the via hole 224 is located at one end of the rear chamber housing 222 away from the negative pressure interface 221, the microporous sheet 25 is located on the side of the via hole 224 away from the negative pressure interface 221, and the front chamber 211 is located on the side of the microporous sheet 25 away from the rear chamber 22, that is, the microporous sheet 25 is located between the front chamber 211 and the rear chamber 22. In other embodiments, the microporous sheet 25 may also be disposed on the side of the via hole 224 close to the negative pressure interface 221.

[0078] Optionally, the microporous sheet 25 is detachably connected to the rear chamber housing 222, that is, the microporous sheet 25 is assembled on the rear chamber housing 222. For example, the microporous sheet 25 forms counting holes through disposable plastic, and the microporous sheet 25 is assembled on the rear chamber housing 222 through glue to serve as a consumable; compared with the existing assembly of the microporous sheet and the reagent kit by ultrasonic technology, the cost can be reduced.

[0079] Optionally, the microporous sheet 25 and the rear chamber housing 222 are integrally formed, that is, the microporous sheet 25 and the rear chamber housing 222 are molded integrally, and the counting holes are provided on the side wall of the rear chamber housing 222 at this time. The integral formation of the microporous sheet 25 and the rear chamber housing 222 in this embodiment can improve the sealing performance of the rear chamber housing 222.

[0080] Compared with the existing assembly of the microporous sheet and the reagent kit by ultrasonic technology, the microporous sheet 25 in this embodiment is detachably connected to the rear chamber housing 222, or the microporous sheet 25 and the rear chamber housing 222 are integrally formed; the assembly method of the microporous sheet 25 and the rear chamber housing 222 is simpler. In addition, the counting holes of the microporous sheet 25 in this embodiment are formed through disposable plastic, and the difference in the aperture of the counting holes between different microporous sheets 25 becomes smaller, that is, the difference in the detection of the same test sample using different rear chambers 22 is small, which is beneficial to reducing the difference in the repeated detection of the same test sample and meeting the repeatability index.

[0081] According to some embodiments of the present application, please refer to Figure 2-5 shown Figure 5 is Figure 2 a schematic structural diagram of an embodiment of the first electrode in

[0082] Among them, the other end of the rear chamber electrode 223 is plugged on the first electrode 261. For example, the other end of the rear chamber electrode 223 is plugged on the first electrode 261, the first electrode 261 is grounded, and the other end of the rear chamber electrode 223 is grounded through the first electrode 261.

[0083] The first electrode 261 includes an elastic sheet, the elastic sheet having a plug-in portion 2611, the cross-sectional area of the plug-in portion 2611 being smaller than the cross-sectional area of the rear cell electrode 223. The other end of the rear cell electrode 223 is inserted into the plug-in portion 2611 so that the plug-in portion 2611 abuts against the other end of the rear cell electrode 223, thereby enabling the other end of the rear cell electrode 223 to be plugged into the plug-in portion 2611.

[0084] For example, the first electrode 261 is made of an elastic sheet, and the two ends of the elastic sheet form the plug-in portion 2611, enabling the other end of the rear cell electrode 223 to be inserted into the plug-in portion 2611.

[0085] The first electrode 261 of this embodiment includes an elastic sheet, and the other end of the rear cell electrode 223 is plugged into the plug-in portion 2611 of the elastic sheet. The structure is simple, facilitating the connection between the rear cell electrode 223 and the first electrode 261.

[0086] Optionally, the kit 21 includes a front cell electrode 212, one end of the front cell electrode 212 being located inside the front cell 211 and the other end of the front cell electrode 212 being located outside the front cell 211. The blood cell counting device 20 further includes a second electrode 262, one end of the second electrode 262 abutting against the other end of the front cell electrode 212, and the other end of the second electrode 262 being connected to the constant current source 263 of the blood cell counting device 20. The constant current source 263 supplies current to the front cell electrode 212 through the second electrode 262.

[0087] Wherein, the second electrode 262 includes a spring thimble, one end of the spring thimble being connected to the constant current source 263 and the other end of the spring thimble abutting against the other end of the front cell electrode 212.

[0088] Optionally, the blood cell counting device 20 further includes a detection component (not shown in the figure), the detection component being connected between the second electrode 262 and the constant current source 263 for collecting signals and processing the signals to detect the sample to be tested located in the kit 21.

[0089] According to some embodiments of the present application, the blood cell counting device 20 further includes a blood cell counting instrument (not shown in the figure) and a kit 21. The blood cell counting instrument includes a pipetting component 23. Wherein, both the kit 21 and the rear cell 22 are consumables, and when the blood cell counting device 20 completes blood cell counting, it is necessary to replace the kit 21 and the rear cell 22.

[0090] The kit 21 includes a front cell 211 and a front cell electrode 212, one end of the front cell electrode 212 being located inside the front cell 211 and the other end of the front cell electrode 212 being located outside the front cell 211.

[0091] Optionally, the first electrode 261, the second electrode 262, the constant current source 263, and the detection component in the above embodiments are all disposed within the blood cell counting device 20. When the blood cell counting device 20 performs blood cell counting, the rear cell 22 and the reagent kit 21 are loaded onto the blood cell counting instrument, and the blood cell counting instrument detects the sample to be tested through the rear cell 22 and the reagent kit 21.

[0092] Optionally, the accessory placement area of the reagent kit 21 is also used to place the rear cell 22; when the blood cell counting device 20 completes blood cell counting, the pipetting component 23 withdraws the rear cell 22 to the accessory placement area, that is, the rear cell 22 is placed in the accessory placement area of the reagent kit 21 to replace the reagent kit 21 and the rear cell 22.

[0093] The blood cell counting device 20 in this embodiment includes a blood cell counting instrument and a reagent kit 21. The rear cell 22 and the reagent kit 21 are loaded onto the blood cell counting instrument, and the blood cell counting instrument detects the sample to be tested through the rear cell 22 and the reagent kit 21 to perform blood cell counting on the sample to be tested.

[0094] The accessory placement area of the reagent kit 21 in this embodiment is used to place the pipette tip 24 and the rear cell 22. In other embodiments, the pipette tip 24 and the rear cell 22 can be placed in other areas of the blood cell counting device 20, such as the pipette tip 24 and the rear cell 22 are placed in the blood cell counting instrument.

[0095] Please refer to Figure 2-6 shown in Figure 6 is Figure 2 a schematic structural diagram of an embodiment of the pipetting component. The pipetting component 23 in this embodiment includes a syringe component 231 and a pipettor 232. The syringe component 231 is connected to the first end of the pipettor 232, and the second end of the pipettor 232 is inserted into the pipette tip 24 or the rear cell 22.

[0096] When the blood cell counting device 20 performs blood cell counting, the second end of the pipettor 232 is in interference connection with the connection end of the rear cell 22. Among them, the connection end of the rear cell 22 is the negative pressure interface 221 in the above embodiment. The interference connection between the second end of the pipettor 232 and the connection end of the rear cell 22 means that the cross-sectional area of the second end of the pipettor 232 is larger than the cross-sectional area of the negative pressure interface 221, so that the second end of the pipettor 232 and the connection end of the rear cell 22 are in interference fit.

[0097] Figure 1 The negative pressure component 12 in [[ ]] usually includes a negative pressure tank (not shown in the figure), an adapter (not shown in the figure), and a valve body (not shown in the figure), resulting in a complex liquid path of the negative pressure component 12. Compared with Figure 1Compared with the negative pressure component 12 in [reference], the pipetting component 23 in this embodiment includes a syringe component 231 and a pipettor 232. The syringe component 231 is connected to the first end of the pipettor 232. The second end of the pipettor 232 is inserted into the pipette tip 24 or the rear chamber 22. The liquid path of the pipetting component 23 is simple, and pipetting and pressure building and counting are achieved through the same pipetting component 23, reducing costs.

[0098] Optionally, please refer to Figure 2-7 as shown in Figure 7 is Figure 2 a schematic structural diagram of an embodiment of the pipettor. The pipettor 232 includes at least a plug-in member 233 and a tip ejector 234. The plug-in member 233 and the tip ejector 234 are located at the second end of the pipettor 232. The plug-in member 233 is used to insert the pipette tip 24 or the rear chamber 22. The tip ejector 234 is sleeved on the plug-in member 233 and can slide in the extending direction of the plug-in member 233.

[0099] The plug-in member 233 includes a first section 2331, a second section 2332, and a third section 2333 that are sequentially connected along the extending direction of the plug-in member 233. The second section 2332 is provided with a groove, and the cross-sectional area of the first section 2331 is larger than the cross-sectional area of the third section 2333. The cross-sectional area of the first section 2331 is larger than the cross-sectional area of the negative pressure interface 221.

[0100] When the plug-in member 233 inserts the pipette tip 24, the pipette tip 24 is sleeved on the third section 2333 and the second section 2332 of the plug-in member 233. When pipetting is completed, the tip ejector 234 is used to eject the pipette tip 24 from the plug-in member 233. When the plug-in member 233 inserts the negative pressure interface 221 of the rear chamber 22, the negative pressure interface 221 is sleeved on the first section 2331, the second section 2332, and the third section 2333 of the plug-in member 233, and the negative pressure interface 221 has an interference fit with the first section 2331. When blood cell counting is completed, the tip ejector 234 is used to eject the rear chamber 22 to the accessory placement area of the reagent kit 21.

[0101] As Figure 6 shown, the syringe component 231 includes a first syringe 2311, a pressure sensor 2312, and a driving member 2313. One end of the first syringe 2311 is connected to the pressure sensor 2312. The pressure sensor 2312 is used to detect the pressure in the first syringe 2311, that is, the pressure in the rear chamber cavity. The driving end of the first syringe 2311 is connected to the driving member 2313. The driving member 2313 is used to drive the first syringe 2311 to aspirate. The other end of the first syringe 2311 is communicated with the channel of the pipettor 232 to communicate with the rear chamber cavity through the channel of the pipettor 232.

[0102] One end of the first syringe 2311 is disposed on the side wall of the first syringe 2311. When pipetting, the connector 233 is inserted into the pipette tip 24, and the driving member 2313 drives the first syringe 2311 to aspirate the pipette tip 24 to suck the diluent into the pipette tip 24; the driving member 2313 drives the first syringe 2311 to push out the pipette tip 24 to push the diluent in the pipette tip 24 into the front chamber 211.

[0103] When performing blood cell counting, the connector 233 is inserted into the negative pressure interface 221 of the rear chamber 22, and the other end of the first syringe 2311 communicates with the rear chamber cavity through the channel of the pipettor 232. The driving member 2313 drives the first syringe 2311 to aspirate the air in the rear chamber cavity to build pressure in the rear chamber 22.

[0104] The following describes the process of the first syringe 2311 building pressure in the rear chamber 22:

[0105] The driving member 2313 controls the first syringe 2311 to perform a first-stage extraction on the rear chamber 22 until the pressure in the rear chamber cavity detected by the pressure sensor 2312 is within the target pressure range. When the pressure in the rear chamber cavity detected by the pressure sensor 2312 is within the target pressure range, the driving member 2313 controls the first syringe 2311 to end the first-stage extraction, improving the accuracy of pressure building. The target pressure range is -20 kPa to -40 kPa, that is, the pressure in the rear chamber cavity can be -20 kPa, -25 kPa, -30 kPa, -35 kPa or -40 kPa.

[0106] The driving member 2313 controls the first syringe 2311 to perform a second-stage extraction on the rear chamber 22 and detect the sample to be tested. When performing blood cell counting, the driving member 2313 controls the first syringe 2311 to perform a second-stage extraction on the rear chamber 22. Among them, the first syringe 2311 quickly extracts the rear chamber 22 in the first stage, and the first syringe 2311 slowly extracts the rear chamber 22 in the second stage.

[0107] In this embodiment, the driving member 2313 controls the first syringe 2311 to perform a first-stage extraction on the rear chamber 22, and the driving member 2313 controls the first syringe 2311 to perform a second-stage extraction on the rear chamber 22 and detect the sample to be tested; there is no need to use the negative pressure assembly 12, reducing costs.

[0108] Optionally, the driving member 2313 controls the first syringe 2311 to perform a second-stage extraction on the rear chamber 22 at a volume extraction rate, and the volume extraction rate is greater than or equal to the pressure drop rate of the first syringe 2311 under the pressure in the rear chamber cavity.

[0109] In this embodiment, by extracting the volumetric velocity greater than or equal to the pressure drop rate of the first syringe 2311 under the pressure of the rear cell cavity, that is, within the same unit time, the volume of the sample to be measured entering the rear cell 22 through the counting hole is less than or equal to the volume extracted by the first syringe 2311 for the second stage extraction of the rear cell 22, so as to increase the pressure of the rear cell cavity, achieve dynamic balance of the pressure of the rear cell cavity, and improve the accuracy of the blood cell counting device 20 for the sample to be measured.

[0110] Optionally, based on the first volume obtained, the driving member 2313 controls the first syringe 2311 to perform the first stage extraction on the rear cell 22, that is, the driving member 2313 controls the first syringe 2311 to extract the first volume from the rear cell 22, so as to achieve the first stage extraction and rapid pressure build-up.

[0111] Optionally, based on the second volume obtained, the driving member 2313 controls the first syringe 2311 to perform the second stage extraction on the rear cell 22, that is, the driving member 2313 controls the first syringe 2311 to extract the second volume from the rear cell 22, so as to achieve the second stage extraction and rapid pressure build-up.

[0112] Please refer to Figure 8 as shown in Figure 8 which is Figure 2 a schematic structural diagram of another embodiment of the pipetting assembly. Different from the Figure 6 syringe assembly 231 shown, the second syringe 2314, three-way joint 2315 and two-way valve 2316 are provided in this embodiment.

[0113] One end of the first syringe 2311 is connected to the first end of the three-way joint 2315, the second syringe 2314 is connected to the second end of the three-way joint 2315 through the two-way valve 2316, and the third end of the three-way joint 2315 is connected to the pressure sensor 2312. The driving ends of the first syringe 2311 and the second syringe 2314 are connected to the driving member 2313, and the driving member 2313 is used to drive the first syringe 2311 and the second syringe 2314 to perform suction simultaneously; the other end of the first syringe 2311 is communicated with the channel of the pipette 232, so as to be communicated with the rear cell cavity through the channel of the pipette 232.

[0114] When pipetting, the plug-in member 233 inserts the pipette tip 24, the two-way valve 2316 is disconnected, the driving member 2313 drives the first syringe 2311 to suck the pipette tip 24, so as to suck the diluent into the pipette tip 24; the driving member 2313 drives the first syringe 2311 to push out the pipette tip 24, so as to push the diluent in the pipette tip 24 to the front cell 211.

[0115] When performing blood cell counting, the connector 233 is inserted into the negative pressure interface 221 of the rear cell 22, the two-way valve 2316 is turned on, and both the first syringe 2311 and the second syringe 2314 are connected to the rear cell cavity through the channel of the pipettor 232. The driving member 2313 drives the first syringe 2311 and the second syringe 2314 to suck the air in the rear cell cavity to build pressure in the rear cell 22.

[0116] Among them, the process of the first syringe 2311 and the second syringe 2314 building pressure in the rear cell 22 is the same as the process of the first syringe 2311 building pressure in the above embodiment, and will not be elaborated here.

[0117] Optionally, the measuring range of the first syringe 2311 is 100 μl, and the measuring range of the second syringe 2314 is 10 ml.

[0118] In this embodiment, the first syringe 2311 sucks the pipette tip 24, and the volume of the diluent inhaled into the pipette tip 24 can be accurately controlled. In this embodiment, the first syringe 2311 and the second syringe 2314 suck the air in the rear cell cavity, and rapid pressure building can be achieved.

[0119] This application also provides a detection method, which is applied to the blood cell counting device 20 disclosed in the above embodiment. Please continue to refer to Figure 9 , Figure 9 is a schematic flow chart of an embodiment of the detection method of this application. The detection method of this embodiment includes the following steps:

[0120] S101: Insert the pipetting assembly 23 into the pipette tip 24, and transfer the sample to the front cell 211 through the pipette tip 24.

[0121] When the blood cell counting device 20 performs pipetting, the pipetting assembly 23 is inserted into the pipette tip 24, and the sample is transferred to the front cell 211 through the pipette tip 24 so that the front cell 211 is loaded with the sample.

[0122] S102: Dilute and / or mix the sample through the pipetting assembly 23 and the pipette tip 24 to obtain a sample to be tested, and withdraw the pipette tip 24 through the pipetting assembly 23.

[0123] The diluent is transferred to the front cell 211 through the pipetting assembly 23 and the pipette tip 24 to perform a dilution operation on the sample in the front cell 211 to obtain a sample to be tested, that is, the front cell 211 is loaded with the sample to be tested.

[0124] Optionally, after the step of diluting the sample in the front cell 211, control the pipetting assembly 23 to blow air bubbles through the pipette tip 24 into the sample and the diluent in the front cell 211 to mix the sample and obtain a sample to be tested.

[0125] S103: Insert the pipetting assembly 23 into the rear cell 22, and connect the front cell 211 to the rear cell 22 through the counting hole to detect the sample to be tested.

[0126] When the blood cell counting device 20 performs blood cell counting, insert the pipetting assembly 23 into the rear cell 22 to build pressure in the rear cell 22; and let the sample to be tested in the front cell 211 flow into the rear cell 22 through the counting hole to detect the sample to be tested.

[0127] During pipetting, the pipetting assembly 23 of this embodiment inserts the pipette tip 24 to perform pipetting and realizes the pipetting function; during blood cell counting, the pipetting assembly 23 is inserted into the rear cell 22 to build pressure in the rear cell 22, that is, the blood cell counting device 20 realizes pipetting and pressure - building counting through the same pipetting assembly 23. The structure is simple, easy to implement, and the cost is reduced.

[0128] Optionally, when the blood cell counting device 20 finishes blood cell counting, control the pipetting assembly 23 to withdraw the rear cell 22 to the accessory placement area of the reagent kit 21 to replace the reagent kit 21 and the rear cell 22 for detecting the next sample to be tested.

[0129] This application also provides a computer - readable storage medium. Please continue to refer to Figure 10 , Figure 10 is a schematic framework diagram of an embodiment of the computer - readable storage medium of this application. The computer - readable storage medium 80 stores program instructions 81. When the program instructions 81 are executed by the processing unit, they are used to implement the method of the above - mentioned embodiment.

[0130] When the embodiments of this application are implemented in the form of software functional units and sold or used as independent products, they can be stored in a computer - readable storage medium. Based on this understanding, the technical solution of this application, in essence, or the part that contributes to the prior art, or all or part of this technical solution, can be embodied in the form of a software product. This computer software product is stored in a storage medium and includes several instructions to enable a computer device (which can be a personal computer, a computer device, or a network device, etc.) or a processor to execute all or part of the steps of the methods described in various embodiments of this application. The foregoing storage medium includes: USB flash drives, mobile hard disks, read - only memories (ROMs), random access memories (RAMs), magnetic disks, or optical discs and other media that can store program codes.

[0131] The above are only the embodiments of the present application, and do not limit the patent scope of the present application accordingly. Any equivalent structure or equivalent process transformation made by using the content of the specification and drawings of the present application, or directly or indirectly applied in other related technical fields, shall be equally included in the patent protection scope of the present application.

Claims

1. A blood cell counting device, characterized in that, Comprising: A front pool, a rear pool, and a counting hole, wherein the front pool is communicated with the rear pool through the counting hole; A pipetting assembly and a pipette tip; When pipetting, the pipetting assembly inserts the pipette tip, and transfers the sample to the front pool through the pipette tip, so that the front pool is loaded with the sample to be tested; When performing blood cell counting, the pipetting assembly inserts the rear pool, and is used for building pressure in the rear pool. The sample to be tested in the front pool flows into the rear pool through the counting hole, so as to detect the sample to be tested.

2. The blood cell counting device according to claim 1, characterized in that, The pipetting assembly includes a syringe assembly and a pipettor. The syringe assembly is connected to the first end of the pipettor, and the second end of the pipettor inserts the pipette tip or the rear pool; when performing blood cell counting, the connection end of the second end of the pipettor and the rear pool is in interference fit.

3. The blood cell counting device according to claim 1 or 2, characterized in that, The rear pool includes a rear pool housing and a rear pool electrode. When performing blood cell counting, the pipetting assembly inserts the rear pool housing to form a rear pool cavity. One end of the rear pool electrode is arranged in the rear pool cavity, and the other end of the rear pool electrode is arranged outside the rear pool cavity.

4. The blood cell counting device according to claim 3, wherein The blood cell counting device is used for detecting a first detection item of the sample to be tested, and the volume of the rear pool cavity is greater than the sum of the volume of the first detection item and a preset remaining volume; Or, the blood cell counting device is used for detecting a second detection item of the sample to be tested, and the volume of the rear pool cavity is greater than the sum of the volume of the second detection item and the preset remaining volume; Or, the blood cell counting device is used for detecting the first detection item and the second detection item of the sample to be tested, and the volume of the rear pool cavity is greater than the sum of the volume of the first detection item, the volume of the second detection item, and the preset remaining volume.

5. The blood cell counting device according to claim 3, characterized in that, The blood cell counting device further includes a microplate. The counting hole is arranged on the microplate. The rear pool housing includes a through hole. The microplate is correspondingly arranged with the through hole, and the microplate is detachably connected or integrally formed with the rear pool housing.

6. The blood cell counting device according to claim 3, characterized in that, The blood cell counting device further includes a first electrode, and the other end of the rear pool electrode is inserted on the first electrode.

7. The blood cell counting device according to claim 6, characterized in that, The first electrode includes an elastic sheet. The elastic sheet has a plugging portion, and the cross-sectional area of the plugging portion is smaller than the cross-sectional area of the rear pool electrode. The other end of the rear pool electrode is inserted into the plugging portion.

8. The blood cell counting device according to claim 3, characterized in that, The blood cell counting device further includes a blood cell counting instrument and a reagent kit. The blood cell counting instrument includes the pipetting assembly. The reagent kit includes a front pool and a front pool electrode. One end of the front pool electrode is located inside the front pool, and the other end of the front pool electrode is located outside the front pool. The reagent kit and the rear pool are loaded in the blood cell counting instrument to detect the sample to be tested.

9. The blood cell counting device according to claim 8, characterized in that, The reagent kit includes an accessory placement area for placing the rear pool; When the blood cell counting is completed, the pipetting assembly withdraws the rear pool to the accessory placement area.

10. A detection method, characterized in that, Applied to the blood cell counting device according to any one of claims 1-9, the detection method includes: Insert the pipetting assembly into the pipette tip and transfer the sample to the front chamber through the pipette tip; Dilute and / or mix the sample through the pipetting assembly and the pipette tip to obtain a sample to be tested, and withdraw the pipette tip through the pipetting assembly; Insert the pipetting assembly into the rear chamber and connect the front chamber to the rear chamber through the counting hole to detect the sample to be tested.

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