Particle counting device and pipetting assembly

Abstract

The invention discloses a particle counting device and a pipetting assembly. The pipetting assembly comprises: a main body part having a first channel; the plugging part is arranged at one end of the main body part and is provided with a second channel, and the second channel is communicated with the first channel; the pipeline is installed on the main body part and the plugging part through the first channel and the second channel, and the first end of the pipeline is far away from the main body part and is exposed out of the plugging part; when the pipetting assembly is used for pipetting, the inserting part is used for inserting a suction head, the first end of the pipeline is located in the suction head, and the pipeline is communicated with the suction head; when the volume of the liquid exceeds the volume of the suction head, the pipeline sucks the liquid through the suction head so as to contain the liquid. Through the mode, the volume of liquid sucked by the pipetting assembly and the suction head is increased, the pipetting frequency of the pipetting assembly is reduced, the detection time of the particle counting device is shortened, and the efficiency is improved.

Description

Technical Field

[0001] This application relates to the technical field of blood cell detection, and particularly to a particle counting device and a pipetting assembly. Background Art

[0002] The blood cell counting device detects a sample to be measured by the impedance method. The blood cell counting device includes a front chamber, a rear chamber, a gemstone aperture, and a pipetting assembly. The gemstone aperture is used to connect the front chamber and the rear chamber, and the pipetting assembly is used to transfer liquid to the front chamber. The sample to be measured in the front chamber flows into the rear chamber through the gemstone aperture to realize the detection of the sample to be measured.

[0003] Among them, the blood cell counting device further includes a pipette tip. When the blood cell counting device performs pipetting, the pipetting assembly sucks the liquid through the pipette tip, and the volume of the pipette tip is small. Assuming that the volume of the liquid pipetted by the blood cell counting device is greater than the volume of the pipette tip, the blood cell counting device needs to perform pipetting multiple times through the pipetting assembly, increasing the detection time of the blood cell counting device and reducing the efficiency. Summary of the Invention

[0004] This application provides a particle counting device and a pipetting assembly to solve the technical problems that occur in the prior art.

[0005] To solve the above problems, a first aspect of this application provides a pipetting assembly, including:

[0006] A main body part having a first channel;

[0007] A plug-in part provided at one end of the main body part, having a second channel, and the second channel communicates with the first channel;

[0008] A pipeline is installed on the main body part and the plug-in part through the first channel and the second channel. The first end of the pipeline is away from the main body part and is exposed outside the plug-in part;

[0009] When the pipetting assembly performs pipetting, the plug-in part is used to plug in the pipette tip, the first end of the pipeline is located inside the pipette tip, and the pipeline communicates with the pipette tip;

[0010] When the volume of the liquid exceeds the volume of the pipette tip, the pipeline sucks the liquid through the pipette tip to place the liquid.

[0011] Among them, the plug-in part includes a plug-in component and a tip ejector. The plug-in component is fixed at one end of the main body part, the tip ejector is sleeved outside the plug-in component, and the tip ejector moves relative to the plug-in component. The first end of the pipeline is exposed outside the plug-in component.

[0012] Among them, the distance range between the first end of the pipeline and the plug-in component is 2-4 mm.

[0013] Wherein, when the connector is not plugged with the pipette tip, the tip-removing member is sleeved on one end of the connector away from the main body under the action of gravity, and the first end of the pipeline is located inside the tip-removing member.

[0014] Wherein, the outer diameter of the pipeline is larger than the inner diameter of the first channel, and the pipeline is in interference fit with the first channel.

[0015] Wherein, the volume of the pipeline is greater than or equal to the sum of the volume of the liquid aspirated by the pipetting assembly and a preset volume, and the preset volume is used to prevent the liquid from flowing out of the pipeline.

[0016] Wherein, the main body includes a sleeve having the first channel, and the pipetting assembly further includes a connector disposed at one end of the sleeve away from the plugging portion.

[0017] Wherein, the pipetting assembly further includes an air pump and a two-way valve, and the air pump is communicated with the second end of the pipeline through the two-way valve; when the pipetting assembly completes pipetting, the air pump blows air into the pipeline through the two-way valve.

[0018] Wherein, the pipetting assembly further includes a syringe and a three-way interface, the first end of the three-way interface is communicated with the second end of the pipeline, the second end of the three-way interface is communicated with the syringe, and the third end of the three-way interface is communicated with the two-way valve.

[0019] To solve the above problems, a second aspect of the present application provides a particle counting device, including:

[0020] A front pool, a rear pool and a counting hole, and the front pool is communicated with the rear pool through the counting hole;

[0021] The above-mentioned pipetting assembly and pipette tip;

[0022] When the pipetting assembly performs pipetting, the pipetting assembly plugs the pipette tip and transfers the liquid to the front pool through the pipette tip.

[0023] The beneficial effects of the present application are as follows: Different from the prior art, the pipetting assembly of the present application includes: a main body portion having a first channel; a plug portion provided at one end of the main body portion and having a second channel, the second channel communicating with the first channel; a pipeline installed on the main body portion and the plug portion through the first channel and the second channel, a first end of the pipeline being away from the main body portion and exposed outside the plug portion; when the pipetting assembly performs pipetting, the plug portion is used to plug a pipette tip, the first end of the pipeline is located inside the pipette tip, and the pipeline communicates with the pipette tip; when the volume of the liquid exceeds the volume of the pipette tip, the pipeline sucks the liquid through the pipette tip to place the liquid. By the above method, when the volume of the liquid exceeds the volume of the pipette tip, the pipeline sucks the liquid through the pipette tip to place the liquid, thereby increasing the volume of the liquid sucked by the pipetting assembly and the pipette tip, reducing the number of pipetting operations of the pipetting assembly, reducing the detection time of the particle counting device, and improving the efficiency. Description of the Drawings

[0024] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the following drawings are only some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings. Among them:

[0025] Figure 1 is a schematic structural diagram of an embodiment of the pipetting assembly of the present application;

[0026] Figure 2 is Figure 1 a schematic structural diagram of an embodiment of the air pump and two-way valve of the pipetting assembly in

[0027] Figure 3 is Figure 1 a schematic structural diagram of an embodiment of the syringe of the pipetting assembly in

[0028] Figure 4 is a schematic structural diagram of an embodiment of the particle counting device of the present application. Detailed Embodiments

[0029] The following will clearly and completely describe the technical solutions in the embodiments of the present application with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only some embodiments of the present application, rather than all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present application.

[0030] The terms "first", "second", "third", "fourth", etc. (if any) in the description, claims and the above-mentioned drawings of this application are used to distinguish similar objects, and do not necessarily describe a specific order or sequence. It should be understood that the data used in this way can be interchanged under appropriate circumstances, so that the embodiments of this application described here, for example, can be implemented in an order other than those illustrated or described here. In addition, the terms "comprising" and "having" and any variations thereof are intended to cover non-exclusive inclusion. For example, a process, method, system, product or device comprising a series of steps or units does not necessarily have to be limited to those steps or units clearly listed, but may include other steps or units not clearly listed or inherent to these processes, methods, products or devices.

[0031] Please refer to Figure 1 as shown Figure 1 is a schematic structural diagram of an embodiment of the pipetting assembly of this application. The pipetting assembly 11 of this embodiment is applied to a particle counting device, and the particle counting device may include a blood cell counting device or a microorganism counting device. This application takes a blood cell counting device as an example for description. The blood cell counting device includes, but is not limited to, a POCT (point-of-care testing) blood cell analyzer or a hematology analyzer.

[0032] In addition, the pipetting assembly 11 of this embodiment is also applicable to fields such as biochemistry or fluorescence, or the pipetting assembly 11 is applied to other fields that require pipetting.

[0033] The pipetting assembly 11 of this embodiment includes a main body part 111, a plug-in part 112 and a pipeline 113. The main body part 111 has a first channel, and the first channel is arranged along the extending direction of the main body part 111; the plug-in part 112 is arranged at one end of the main body part 111. For example, the plug-in part 112 and the main body part 111 are detachably connected or integrally formed.

[0034] The plug-in part 112 has a second channel, and the second channel is arranged along the extending direction of the plug-in part 112, and the second channel is communicated with the first channel. When the plug-in part 112 and the main body part 111 are connected, the second channel is communicated with the first channel; for example, the first through hole and the second through hole are coaxially designed.

[0035] The pipeline 113 is installed on the main body part 111 and the plug-in part 112 through the first through hole and the second through hole, that is, the pipeline 113 is arranged in the first through hole and the second through hole. The first end of the pipeline 113 is far from the main body part 111, the second end of the pipeline 113 is close to the main body part 111, and the first end of the pipeline 113 is exposed outside the plug-in part 112. The first end of the pipeline 113 being exposed outside the plug-in part 112 means that the first end of the pipeline 113 protrudes relative to the plug-in part 112, that is, the first end of the pipeline 113 is located in the extending direction of the plug-in part 112.

[0036] When the pipetting assembly 11 performs pipetting, that is, when the blood cell counting device performs pipetting, the pipetting assembly 11 inserts the pipette tip 12, that is, the insertion part 112 inserts the pipette tip 12. The insertion part 112 inserting the pipette tip 12 means that the insertion part 112 is inserted into the pipette tip 12 to connect the insertion part 112 with the pipette tip 12. Among them, the pipette tip 12 is a consumable, and the pipette tip 12 can also be called a tip head; when the blood cell counting device completes blood cell counting, the pipette tip 12 needs to be replaced.

[0037] The insertion part 112 inserts the pipette tip 12, that is, the first end of the pipeline 113 is located inside the pipette tip 12, and the first end of the pipeline 113 communicates with the pipette tip 12.

[0038] The blood cell counting device obtains the volume of the liquid required during pipetting and compares the volume of the liquid with the volume of the pipette tip 12. When the volume of the liquid does not exceed the volume of the pipette tip 12, the pipetting assembly 11 aspirates the liquid through the pipette tip 12, and the liquid is placed in the pipette tip 12. When the volume of the liquid exceeds the volume of the pipette tip 12, since the pipeline 113 communicates with the pipette tip 12, the pipeline 113 aspirates the liquid through the pipette tip 12 so that part of the liquid is placed in the pipette tip 12 and the rest of the liquid is placed in the pipeline 113 to place the liquid.

[0039] For example, the blood cell counting device needs to pipette the dilution liquid through the pipetting assembly 11 and the pipette tip 12, and the pipetting assembly 11 aspirates the dilution liquid through the pipette tip 12 to transfer the dilution liquid to the front pool of the blood cell counting device.

[0040] In this embodiment, when the volume of the liquid exceeds the volume of the pipette tip 12, the pipeline 113 aspirates the liquid through the pipette tip 12 to place the liquid, thereby increasing the volume of the liquid aspirated by the pipetting assembly 11 and the pipette tip 12, reducing the number of pipetting times of the pipetting assembly 11, reducing the detection time of the blood cell counting device, and improving the efficiency.

[0041] According to some embodiments of the present application, as Figure 1 shown, the insertion part 112 of this embodiment includes a connector 1121 and a tip ejector 1122. The connector 1121 is fixed to one end of the main body part 111, and the tip ejector 1122 is sleeved outside the connector 1121.

[0042] Among them, the tip ejector 1122 can move relative to the connector 1121, that is, the tip ejector 1122 moves relative to the connector 1121 along the extension direction of the connector 1121.

[0043] For example, the plug-in part 112 further includes a slider (not shown in the figure) and a track (not shown in the figure). One of the plug-in connector 1121 and the head-removing part 1122 is provided with the slider, and the other of the plug-in connector 1121 and the head-removing part 1122 is provided with the track. The slider and the track are located between the plug-in connector 1121 and the head-removing part 1122. Therefore, the head-removing part 1122 can relatively slide with respect to the plug-in connector 1121 through the slider and the track. In other embodiments, a slide rail is provided between the plug-in connector 1121 and the head-removing part 1122, so that the head-removing part 1122 can relatively slide with respect to the plug-in connector 1121 through the slider and the track.

[0044] When the plug-in part 112 plugs the suction head 12, under the action of the suction force of the suction head 12, the head-removing part 1122 moves away from the suction head 12 with respect to the plug-in connector 1121, so that the plug-in connector 1121 can plug the suction head 12. At this time, the first end of the pipeline 113 is exposed outside the plug-in connector 1121, and the first end of the pipeline 113 is exposed outside the head-removing part 1122.

[0045] When the plug-in part 112 withdraws from the suction head 12, under the action of an external force, the head-removing part 1122 moves towards the suction head 12 with respect to the plug-in connector 1121, and the head-removing part 1122 is used to withdraw the suction head 12 from the plug-in connector 1121.

[0046] The plug-in part 112 of this embodiment includes a plug-in connector 1121 and a head-removing part 1122. The plug-in connector 1121 is fixed to one end of the main body part 111, and the head-removing part 1122 is sleeved outside the plug-in connector 1121. The plug-in connector 1121 is used to plug the suction head 12, and the head-removing part 1122 is used to withdraw the suction head 12 from the plug-in connector 1121, so as to realize the plugging or withdrawal of the suction head 12 by the plug-in part 112. The structure is simple and the cost is reduced.

[0047] Optionally, the liquid transfer assembly 11 further includes a moving part 114. The main body part 111 is arranged on the moving part 114, and the moving part 114 is used to drive the main body part 111 and the plug-in part 112 to move along the extending direction of the main body part 111.

[0048] For example, when the plug-in part 112 plugs the suction head 12, the moving part 114 drives the main body part 111 and the plug-in part 112 to move towards the suction head 12, so that the plug-in connector 1121 can plug the suction head 12. When the liquid transfer assembly 11 performs liquid transfer, the moving part 114 drives the main body part 111 and the plug-in part 112 to move away from the suction head 12, so as to move the suction head 12 with the sucked liquid to the front pool of the blood cell counting device.

[0049] Optionally, the moving part 114 includes a pushing member 1141, and the bump 1123 of the head-removing member 1122 is disposed within the pushing member 1141. When the plugging part 112 plugs the suction head 12, the bump 1123 of the head-removing member 1122 can move within the pushing member 1141 in a direction away from the suction head 12. When the plugging part 112 withdraws from the suction head 12, the pushing member 1141 pushes the head-removing member 1122 in a direction close to the suction head 12 through the bump 1123 to withdraw the suction head 12 from the plug 1121. Among them, the pushing member 1141 includes but is not limited to a driving motor.

[0050] According to some embodiments of the present application, when the plug 1121 is not plugged with the suction head 12, the head-removing member 1122 is sleeved on one end of the plug 1121 away from the main body part 111 under the action of gravity, and the first end of the pipeline 113 is located within the head-removing member 1122.

[0051] Among them, when the plug 1121 is not plugged with the suction head 12, the head-removing member 1122 is sleeved on one end of the plug 1121 away from the main body part 111 due to the gravity of the head-removing member 1122. At this time, the first end of the pipeline 113 is located within the head-removing member 1122. That is, the first end of the pipeline 113 is exposed outside the plug 1121 and is located within the head-removing member 1122.

[0052] In this embodiment, the head-removing member 1122 is sleeved on one end of the plug 1121 away from the main body part 111 under the action of gravity, and the first end of the pipeline 113 is located within the head-removing member 1122; the head-removing member 1122 can protect the first end of the pipeline 113 and prevent the first end of the pipeline 113 from being hit by other objects.

[0053] According to some embodiments of the present application, the distance range between the first end of the pipeline 113 and the plug 1121 is 2-4 mm. The distance between the first end of the pipeline 113 and the plug 1121 can be 2 mm, 3 mm, or 4 mm.

[0054] For example, the distance between the first end of the pipeline 113 and the plug 1121 is 3 mm. When the plug 1121 plugs the suction head 12, the distance of the first end of the pipeline 113 within the suction head 12 is 3 mm.

[0055] In the prior art, the pipetting assembly does not have a pipeline exposed outside the pipetting assembly. That is, the distance between the first end of the pipeline 113 and the connector 1121 is 0 mm (i.e., less than 2 mm), and the first end of the pipeline 113 is located inside the connector 1121, which is not convenient for sucking the liquid in the pipette tip 12 into the pipeline 113. Suppose the distance between the first end of the pipeline 113 and the connector 1121 is too large (e.g., 5 mm). At this time, the length of the first end of the pipeline 113 located inside the pipette tip 12 is too long, resulting in the distance between the first end of the pipeline 113 and the suction end of the pipette tip 12 being too close, which affects the liquid suction of the pipette tip 12. Therefore, in this embodiment, the distance range between the first end of the pipeline 113 and the connector 1121 is 2 - 4 mm. Since the first end of the pipeline 113 is located inside the pipette tip 12, it is convenient to suck the liquid in the pipette tip 12 into the pipeline 113; in addition, there is a certain distance between the first end of the pipeline 113 and the suction end of the pipette tip 12, avoiding affecting the liquid suction of the pipette tip 12.

[0056] According to some embodiments of the present application, the outer diameter of the pipeline 113 is greater than the inner diameter of the first channel of the main body portion 111, and the pipeline 113 is in interference fit with the first channel, that is, the pipeline 113 is in interference fit with the main body portion 111.

[0057] In this embodiment, the outer diameter of the pipeline 113 is greater than the inner diameter of the first channel of the main body portion 111, and the pipeline 113 is in interference fit with the first channel, so that the pipeline 113 is tightly fitted with the main body portion 111, preventing the pipeline 113 from falling off the main body portion 111.

[0058] According to some embodiments of the present application, the volume of the pipeline 113 is greater than or equal to the sum of the volume of the liquid sucked by the pipetting assembly 11 and a preset volume, and the preset volume is used to prevent the liquid from flowing out of the pipeline 113.

[0059] The volume of the liquid sucked by the pipetting assembly 11 refers to the volume of the liquid required by the blood cell counting device during the pipetting process. The preset volume is a pre-set volume, and by setting the preset volume, it is possible to prevent the liquid from flowing out of the second end of the pipeline 113.

[0060] For example, if the volume of the liquid sucked by the pipetting assembly 11 is 1000 μl and the preset volume is 100 μl, then the volume of the pipeline 113 is greater than or equal to 1100.

[0061] In this embodiment, the volume of the pipeline 113 is greater than or equal to the sum of the volume of the liquid sucked by the pipetting assembly 11 and the preset volume, which can prevent the liquid from flowing out of the pipeline 113.

[0062] According to some embodiments of the present application, the inner diameter range of the pipeline 113 is 1 - 2.5 mm, and the inner diameter of the pipeline 113 is 1 mm, 1.5 mm, 2 mm or 2.5 mm. For example, the inner diameter of the pipeline 113 is 1.5 mm.

[0063] Assume that the inner diameter of the pipeline 113 is too small (for example, the inner diameter of the pipeline 113 is less than 1 mm), resulting in slow response and low efficiency of the pipeline 113 in sucking liquid; assume that the inner diameter of the pipeline 113 is too large (for example, the inner diameter of the pipeline 113 is less than 2.5 mm), resulting in low accuracy of the pipeline 113 in sucking liquid and inaccurate amount of sucked liquid. The inner diameter range of the pipeline 113 in this embodiment is 1 - 2.5 mm, which improves the response speed of the pipeline 113 in sucking liquid, improves the efficiency and the accuracy of sucking liquid, and improves the amount of liquid sucked by the pipeline 113.

[0064] According to some embodiments of the present application, the main body 111 includes a sleeve 1111 which has a first channel, and the pipetting assembly 11 further includes a connector 110 which is arranged at one end of the sleeve 1111 away from the plug portion 112.

[0065] The sleeve 1111 is arranged on the moving part 114, the plug portion 112 is arranged at one end of the sleeve 1111, the connector 110 is arranged at the other end of the sleeve 1111, and the other end of the sleeve 1111 is the end away from the plug portion 112.

[0066] Optionally, a groove is provided at the other end of the sleeve 1111, and the connector 110 is arranged on the groove of the sleeve 1111. The pipeline 123 passes through the connector 110, the first channel and the second channel to be arranged on the connector 110, the sleeve 1111 and the plug 1121.

[0067] The pipetting assembly 11 in this embodiment further includes a connector 110 which is arranged at one end of the sleeve 1111 away from the plug portion 112, to seal the sleeve 1111 and improve the airtightness of the sleeve 1111; in addition, the connector 110 is also used to fix the pipeline 123 on the sleeve 1111.

[0068] According to some embodiments of the present application, as shown in FIGS. 1 - 2, Figure 2 is Figure 1 a schematic structural diagram of an embodiment of the air pump and the two - way valve of the pipetting assembly. The pipetting assembly 11 in this embodiment further includes an air pump 115 and a two - way valve 116, and the air pump 115 is communicated with the second end of the pipeline 113 through the two - way valve 116.

[0069] When the pipetting assembly 11 performs pipetting, the two - way valve 116 is disconnected, and at this time, the air pump 115 is not communicated with the second end of the pipeline 113. When the pipetting assembly 11 completes pipetting, that is, when the pipetting assembly 11 discharges the liquid in the pipeline 113 and the pipette tip 12, the two - way valve 116 is turned on, and at this time, the air pump 115 is communicated with the second end of the pipeline 113 through the two - way valve 116, and the air pump 115 blows air through the two - way valve 116 to the pipeline 113 to blow out the liquid in the pipeline 113 and the pipette tip 12 into the kit that has completed the detection in the blood cell counting device.

[0070] The pipetting assembly 11 of this embodiment further includes an air pump 115 and a two-way valve 116. The air pump 115 is connected to the second end of the pipeline 113 through the two-way valve 116. When the pipetting assembly 11 completes pipetting, the air pump 115 blows air into the pipeline 113 through the two-way valve 116, which can blow out the liquid in the pipeline 113 and the pipette tip 12, avoiding the liquid in the pipeline 113 and the pipette tip 12 from contaminating other samples to be tested.

[0071] According to some embodiments of the present application, as shown in FIGS. 1 and 3, Figure 3 is Figure 1 a schematic structural diagram of an embodiment of the syringe of the pipetting assembly. The pipetting assembly 11 of this embodiment further includes a syringe 117 and a three-way interface 118. The first end of the three-way interface 118 is connected to the second end of the pipeline 113, the second end of the three-way interface 118 is connected to the syringe 117, and the third end of the three-way interface 118 is connected to the two-way valve 116.

[0072] When the pipetting assembly 11 performs pipetting, the two-way valve 116 is disconnected, and the air pump 115 is not connected to the second end of the pipeline 113. The syringe 117 is connected to the second end of the pipeline 113 through the three-way interface 118, and the syringe 117 is used to suck liquid through the pipeline 113 and the pipette tip 12.

[0073] Optionally, the pipetting assembly 11 further includes a driving member 119. The driving member 119 is connected to the driving end of the syringe 117 and is used to drive the syringe 117 to suck the pipeline 113 and the pipette tip 12.

[0074] The pipetting assembly 11 of this embodiment further includes a syringe 117 and a three-way interface 118. The first end of the three-way interface 118 is connected to the second end of the pipeline 113, the second end of the three-way interface 118 is connected to the syringe 117, and the third end of the three-way interface 118 is connected to the two-way valve 116. By sucking the pipeline 113 and the pipette tip 12 through the syringe 117, the pipette tip 12 sucks liquid. When the volume of the liquid exceeds the volume of the pipette tip 12, the pipeline 113 sucks liquid through the pipette tip 12 to place the liquid, thereby increasing the volume of liquid sucked by the pipetting assembly 11 and the pipette tip 12, reducing the number of pipetting operations of the pipetting assembly 11, reducing the detection time of the blood cell counting device, and improving the efficiency.

[0075] The present application also provides a particle counting device. Please refer to Figure 1-4 shown in Figure 4 is a schematic structural diagram of an embodiment of the particle counting device of the present application.

[0076] In this embodiment, the particle counting device is described as a blood cell counting device. The blood cell counting device includes a front pool 13, a rear pool 14, a counting hole (not shown in the figure), a pipetting assembly 11, and a pipette tip 12.

[0077] Among them, the front chamber 13 and the rear chamber 14 are connected through a counting hole. When the blood cell counting device performs blood cell counting, the front chamber 13 is used to load the sample to be tested, and the counting hole is used to allow the blood cells in the sample to be tested in the front chamber 13 to pass through one by one. The diameter of the counting hole can be correspondingly set according to the different particle sizes of the blood cells.

[0078] Optionally, the blood cell counting device further includes a reagent kit 21, and the front chamber 13 is arranged on the reagent kit 21. The reagent kit 21 may further include an accessory placement area, and the pipette tip 12 is placed in the accessory placement area.

[0079] When the blood cell counting device performs pipetting, the pipetting assembly 11 is plugged with the pipette tip 12, and the liquid is transferred to the front chamber 13 through the pipette tip 12 so that the front chamber 13 is loaded with the sample to be tested. When the blood cell counting device performs blood cell counting, the pipetting assembly 11 is plugged with the rear chamber 14 to build pressure on the rear chamber 14, and the sample to be tested in the front chamber 13 flows into the rear chamber 14 through the counting hole to detect the sample to be tested.

[0080] The blood cell counting device of this embodiment realizes pipetting and pressure building for counting through the same pipetting assembly 11, without two sets of liquid paths, has a simple structure, is easy to implement, and reduces costs.

[0081] Optionally, the rear chamber 14 of this embodiment includes a rear chamber housing 141 and a rear chamber electrode 142, and the rear chamber housing 141 has a negative pressure interface 143. When the blood cell counting device performs blood cell counting, the pipetting assembly 11 is plugged with the negative pressure interface 143. The pipetting assembly 11 and the rear chamber housing 141 form a rear chamber cavity, and the rear chamber cavity is used to place the sample to be tested flowing through the counting hole.

[0082] The rear chamber electrode 142 is fixed on the rear chamber housing 141. One end of the rear chamber electrode 142 is located inside the rear chamber housing 141, and the other end of the rear chamber electrode 142 is located outside the rear chamber housing 141.

[0083] Optionally, the blood cell counting device can be used to perform a first test item and / or a second test item on the sample to be tested. The first test item includes but is not limited to RBC (Red Blood Cell) test item and / or PLT (Platelet count) test item; the second test item includes but is not limited to WBC (white blood cell) test item.

[0084] Optionally, the blood cell counting device is used to perform the first test item and / or the second test item on the sample to be tested, and the volume of the rear chamber cavity is greater than the sum of the volume of the first test item and / or the volume of the second test item and a preset remaining volume.

[0085] For example, the first test item is the RBC test item, and the second test item is the WBC test item. The blood cell counting device is used to perform the RBC test item and the WBC test item on the sample to be tested. Then, the volume of the rear pool cavity is greater than the sum of the volume of the RBC test item, the volume of the WBC test item, and the preset remaining volume. Among them, the volume of the RBC test item is 180 μl, the volume of the WBC test item is 450 μl, and the preset remaining volume is 100 μl. Then, the volume of the rear pool cavity is greater than 730 μl.

[0086] The volume of the rear pool cavity in this embodiment is greater than the sum of the volume of the first test item and / or the volume of the second test item and the preset remaining volume, which can ensure that the liquid transfer component 11 is in contact with the waste liquid in the rear pool cavity and avoid the liquid transfer component 11 being contaminated by the waste liquid in the rear pool cavity.

[0087] Optionally, as Figure 3-4 shown, the syringe 117 is further connected to the pressure sensor 120, and the pressure sensor 120 is used to detect the pressure of the rear pool cavity. The following describes the process of the syringe 117 building pressure in the rear pool 14:

[0088] The driving member 119 controls the syringe 117 to perform the first-stage extraction on the rear pool 14 until the pressure of the rear pool cavity detected by the pressure sensor 120 is within the target pressure range. When the pressure of the rear pool cavity detected by the pressure sensor 120 is within the target pressure range, the driving member 119 controls the syringe 117 to end the first-stage extraction, improving the accuracy of pressure building. The target pressure range is -20 kPa to -40 kPa, that is, the pressure of the rear pool cavity can be -20 kPa, -25 kPa, -30 kPa, -35 kPa, or -40 kPa.

[0089] The driving member 119 controls the syringe 117 to perform the second-stage extraction on the rear pool 14 and detect the sample to be tested. When performing blood cell counting, the driving member 119 controls the syringe 117 to perform the second-stage extraction on the rear pool 14. Among them, the syringe 117 rapidly extracts the rear pool 14 in the first stage, and the syringe 117 slowly extracts the rear pool 14 in the second stage.

[0090] In this embodiment, the driving member 119 controls the syringe 117 to perform the first-stage extraction on the rear pool 14, and the driving member 119 controls the syringe 117 to perform the second-stage extraction on the rear pool 14 and detect the sample to be tested; there is no need to use a negative pressure component, reducing costs.

[0091] Optionally, the driving member 119 controls the syringe 117 to perform the second-stage extraction on the rear pool 14 at a pumping volume speed, and the pumping volume speed is greater than or equal to the pressure drop rate of the syringe 117 under the pressure of the rear pool cavity.

[0092] In this embodiment, by extracting the volumetric velocity greater than or equal to the pressure drop rate of the syringe 117 under the pressure in the rear cell cavity, that is, within the same unit time, the volume of the sample to be measured entering the rear cell 14 through the counting hole is less than or equal to the volume of the second-stage extraction of the rear cell 14 by the syringe 117, so as to increase the pressure in the rear cell cavity and achieve dynamic balance of the pressure in the rear cell cavity, thereby improving the accuracy of the blood cell counting device for the sample to be measured.

[0093] Optionally, based on the first volume obtained, the driving member 119 controls the syringe 117 to perform a first-stage extraction on the rear cell 14, that is, the driving member 119 controls the syringe 117 to extract a first volume from the rear cell 14 to achieve the first-stage extraction and rapid pressure build-up.

[0094] Optionally, based on the second volume obtained, the driving member 119 controls the syringe 117 to perform a second-stage extraction on the rear cell 14, that is, the driving member 119 controls the syringe 117 to extract a second volume from the rear cell 14 to achieve the second-stage extraction and rapid pressure build-up.

[0095] In summary, when the volume of the liquid exceeds the volume of the pipette tip 12 in this application, the pipeline 113 sucks the liquid through the pipette tip 12 to place the liquid, thereby increasing the volume of the liquid sucked by the pipetting assembly 11 and the pipette tip 12, reducing the number of pipetting operations of the pipetting assembly 11, shortening the detection time of the particle counting device, and improving the efficiency.

[0096] The above are only the embodiments of this application, and do not limit the patent scope of this application accordingly. Any equivalent structure or equivalent process transformation made by using the content of the specification and drawings of this application, or directly or indirectly applied in other related technical fields, shall be equally included in the patent protection scope of this application.

Claims

1. A pipetting assembly, characterized in that, Comprising: A main body part having a first channel; A plug-in part provided at one end of the main body part and having a second channel, the second channel communicating with the first channel; A pipeline installed on the main body part and the plug-in part through the first channel and the second channel, a first end of the pipeline being away from the main body part and exposed outside the plug-in part; When the liquid transfer assembly performs liquid transfer, the plug-in part is used to plug a pipette tip, the first end of the pipeline is located inside the pipette tip, and the pipeline communicates with the pipette tip; When the volume of the liquid exceeds the volume of the pipette tip, the pipeline sucks the liquid through the pipette tip to place the liquid.

2. The pipetting assembly according to claim 1, wherein The plug-in part includes a plug-in component and a tip-removing part. The plug-in component is fixed to one end of the main body part. The tip-removing part is sleeved outside the plug-in component, and the tip-removing part moves relative to the plug-in component. The first end of the pipeline is exposed outside the plug-in component.

3. The pipetting assembly according to claim 2, wherein, The distance range between the first end of the pipeline and the plug-in component is 2 - 4 mm.

4. The pipetting assembly according to claim 2, characterized in that, When the plug-in component does not plug the pipette tip, the tip-removing part is sleeved on the end of the plug-in component away from the main body part under the action of gravity, and the first end of the pipeline is located inside the tip-removing part.

5. The pipetting assembly according to claim 1, wherein The outer diameter of the pipeline is larger than the inner diameter of the first channel, and the pipeline is in interference fit with the first channel.

6. The pipetting assembly according to any one of claims 1-5, characterized in that, The volume of the pipeline is greater than or equal to the sum of the volume of the liquid sucked by the liquid transfer assembly and a preset volume, and the preset volume is used to prevent the liquid from flowing out of the pipeline.

7. The pipetting assembly according to any one of claims 1-5, characterized in that, The main body part includes a sleeve having the first channel. The liquid transfer assembly further includes a connector provided at the end of the sleeve away from the plug-in part.

8. The pipetting assembly according to any one of claims 1-5, characterized in that, The liquid transfer assembly further includes an air pump and a two-way valve. The air pump communicates with the second end of the pipeline through the two-way valve; when the liquid transfer assembly completes liquid transfer, the air pump blows air into the pipeline through the two-way valve.

9. The pipetting assembly according to claim 8, wherein, The liquid transfer assembly further includes a syringe and a three-way interface. The first end of the three-way interface communicates with the second end of the pipeline, the second end of the three-way interface communicates with the syringe, and the third end of the three-way interface communicates with the two-way valve.

10. A particle counting device, characterized in that, Comprising: A front pool, a rear pool and a counting hole, the front pool communicating with the rear pool through the counting hole; The liquid transfer assembly and the pipette tip according to any one of claims 1 - 9; When the liquid transfer assembly performs liquid transfer, the liquid transfer assembly plugs the pipette tip and transfers the liquid to the front pool through the pipette tip.

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