A device for detecting a biological macromolecule

Abstract

The application discloses a kind of biological macromolecule detection devices, characterized by including detection pedestal, sample loading positioning mechanism is provided on detection pedestal, sample image capture mechanism and light source mechanism, biological macromolecule sample to be detected is positioned by sample loading positioning mechanism, light source mechanism provides illuminating light source for the biological macromolecule sample to be measured positioned and installed on sample loading positioning mechanism, and the image information of biological macromolecule sample to be measured is captured by sample image capture mechanism.The advantage is to effectively solve the core pain points of inaccurate manual interpretation and low efficiency in traditional biological macromolecule detection, with high precision, flexibility and low cost advantage.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological detection, in particular to a biological macromolecule detection device. BACKGROUND

[0002] In the existing biological detection technology, there are many detection products with convenient operation, such as colloidal gold detection test strips, non-fluorescently labeled biochips, etc. For the detection results of biological macromolecules visible to the naked eye, human judgment is currently basically adopted. Human judgment often cannot accurately quantitatively judge, especially when the result is weakly positive. Due to the error of human judgment, it is easy to lead to inconsistency of the result. In addition, human judgment is time-consuming and labor-consuming, and the efficiency is also relatively low. SUMMARY

[0003] The technical problem to be solved by the present application is to provide a biological macromolecule detection device capable of reducing human judgment error and providing rapid and accurate detection results.

[0004] The technical scheme adopted by the present application to solve the above technical problem is:

[0005] A biological macromolecule detection device, comprising a detection base, wherein a sample loading and positioning mechanism, a sample image capturing mechanism and a light source mechanism are arranged on the detection base, a sample to be detected is positioned through the sample loading and positioning mechanism, the light source mechanism provides an illumination light source for the sample positioned on the sample loading and positioning mechanism, and the image information of the sample is captured through the sample image capturing mechanism.

[0006] The sample loading and positioning mechanism comprises a loading carrier and a positioning seat, the positioning seat has an inner cavity and an image information capturing opening arranged at the front end and communicating with the inner cavity, the loading carrier is inserted into the inner cavity to align the sample with the image information capturing opening, and the light source mechanism is arranged in the inner cavity and located in front of the loading carrier.

[0007] The sample image capturing mechanism comprises a mobile phone with detection software and a mobile phone positioning support, the mobile phone positioning support comprises a shooting calibration frame which is detachably arranged on the positioning seat, the shooting calibration frame has a through cavity, left and right frame limiting pieces are slidably arranged in the through cavity, and an adjustable image capturing hole is formed.

[0008] The positioning seat is provided with a limiting mechanism for limiting the left and right frame limiting pieces to align the image capturing hole with the image information capturing opening.

[0009] The positioning seat is provided with a positioning slot for positioning and installing the loading carrier into the inner cavity, and the loading carrier is inserted into the positioning seat through the positioning slot to align the sample to be detected with the image information capturing port.

[0010] The image information capturing port is detachably provided with a filter.

[0011] The filter has a mounting frame, the filter is mounted in the mounting frame, the mounting frame is detachably embedded in the image information capturing port, a corresponding area of the filter is an image information capturing area, and the loading carrier is inserted into the positioning seat through the positioning slot to align the sample to be detected with the image information capturing area.

[0012] The image information capturing port is provided with a positioning and installing column with an inner thread hole, a positioning screw is screwed on the positioning and installing column through a corresponding mounting hole provided on the mounting frame, and the detachable mounting of the filter on the image information capturing port is realized.

[0013] The light source mechanism is a multi-wavelength LED light bar.

[0014] The LED light bar is provided with LED patch lamp beads of various wavelengths, different lamp beads can be controlled to emit light according to needs through a control circuit, to emit light of fixed wavelengths for exciting different fluorescent substances in the biological macromolecule sample to be detected, for example, light of 375 nm and 480 nm wavelengths can correspondingly excite SYBR Green I fluorescent substances.

[0015] The mobile phone positioning support further includes a supporting bracket, the shooting calibration frame is used to align the camera of the mobile phone with the image information capturing port, the supporting bracket is used to support the bottom of the mobile phone, and the supporting bracket is adjustably arranged on the detection base in an up-down direction.

[0016] The limiting mechanism includes a left limiting tab protruding from the front end surface of the positioning seat and used for limiting the left frame limiting piece, a right limiting tab protruding from the front end surface of the positioning seat and used for limiting the right frame limiting piece, and the image information capturing port is located between the left limiting tab and the right limiting tab, the front end surface of the positioning seat is provided with an upper limiting plate used for forming upper limiting installation of the shooting calibration frame and a lower limiting plate used for forming lower limiting installation of the shooting calibration frame, the shooting calibration frame is embedded between the upper limiting plate and the lower limiting plate after installation, the left frame limiting piece is attached to the outer side of the left limiting tab, and the right frame limiting piece is attached to the outer side of the right limiting tab.

[0017] The middle part of the left frame piece and the right frame piece is outwardly convex to form an arc-shaped convex part, and the two convex parts are mirror-symmetrically arranged.

[0018] The upper and lower inner end faces of the through cavity are respectively provided with a sliding groove in the left-right direction, and the upper and lower ends of the left frame piece and the right frame piece are respectively slidably installed in the corresponding sliding groove. At least one of the sliding grooves is detachably provided with a locking buckle for locking the sliding position of the left frame piece and the right frame piece, respectively. After installation, the inner and outer sides of the left frame piece are limited by the corresponding locking buckle and the left limiting tab, and the inner and outer sides of the right frame piece are limited by the corresponding locking buckle and the right limiting tab.

[0019] The shooting calibration frame is composed of two opposite left-right movable clamping arms, and an elastic reset element is arranged between the two clamping arms to form a clamping cavity for clamping the mobile phone.

[0020] The elastic reset element is a reset spring.

[0021] A guide rod is arranged on the detection base, a matching guide hole is arranged on the supporting bracket, the supporting bracket is installed on the detection base in the up-down height position by cooperation of the guide hole and the guide rod, and a locking mechanism is arranged between the supporting bracket and the detection base to lock the position of the supporting bracket.

[0022] The locking mechanism includes a locking bolt and a locking plate. The locking plate is arranged in the detection base, a sliding groove is arranged in the detection base along the movement direction of the supporting bracket to slidably install the locking plate, the locking plate is slidably arranged in the sliding groove synchronously with the movement of the supporting bracket, the locking plate is provided with a locking hole matched with the locking bolt, a guide groove is arranged in the front end face of the detection base along the movement direction of the supporting bracket and connected with the sliding groove, the locking bolt is arranged on the supporting bracket, and the rod part of the locking bolt is screwed on the locking plate through the guide groove. In the locked state, the locking bolt is in the tightened state, the head part of the locking bolt is pressed on the front end face of the supporting bracket, and the rear end face of the supporting bracket is abutted on the detection base.

[0023] The front part of the detection base is provided with a concave cavity, and the guide rod is installed in the concave cavity.

[0024] The positioning seat is obliquely supported on the detection base, and the guide rod is obliquely arranged on the detection base, and the inclination angle of the positioning seat and the guide rod is the same.

[0025] The detection base is provided with a rechargeable battery, and the detection base is provided with a matching charging interface.

[0026] The detection base is provided with a light control circuit, and the detection base is provided with a light control switch electrically connected with the light control circuit.

[0027] Compared with the prior art, the device effectively solves the core pain points of inaccurate manual interpretation and low efficiency in traditional biological macromolecule detection, and has the advantages of high precision, flexibility and low cost.

[0028] (1) The image capture mechanism (mobile phone camera) combined with the detection software replaces manual interpretation, solves the problem of weak positive results difficult to quantify, and significantly reduces human error;

[0029] (2) The light source mechanism adopts a multi-wavelength LED light bar (such as 375nm and 480nm), which can adapt to different fluorescent markers (such as SYBR Green I), realize specific excitation, and improve detection sensitivity and accuracy;

[0030] (3) The filter is detachably installed in the image information capture port through the mounting frame, which can adapt to different fluorescent detection requirements to realize quick replacement of the matching filter;

[0031] (4) The positioning slot and the image information capture port cooperate with the shooting calibration frame to ensure that the sample to be tested is aligned with the mobile phone camera, and operation deviation is avoided;

[0032] (5) The mobile phone camera is used as the image capture core, and the shooting calibration frame (including a slidable clamping arm and an elastic return spring) is used to adapt to different sizes of mobile phones for clamping to ensure stable shooting;

[0033] (6) The mobile phone is matched with the matching detection software to realize automatic image analysis and improve efficiency;

[0034] (7) The support bracket realizes fine height adjustment through the guide rod and the locking bolt to adapt to different mobile phone sizes and user operation habits;

[0035] (8) The positioning seat and the guide rod are inclined at the same angle, which is convenient for observation and operation;

[0036] (9) Built-in rechargeable battery and light control circuit, support outdoor or no power environment, enhance the practicality;

[0037] (10) Light source mechanism front, suitable for use in fluorescence detection scene, optimize the fluorescence signal;

[0038] (11) Limiting tab and split frame design provides multi-dimensional calibration (left and right, up and down), significantly improves the alignment accuracy of the camera of the mobile phone and the sample plane, and can quickly determine the position of the camera of the mobile phone to be used, and after locking, the shooting calibration frame is positioned and installed on the positioning seat, so that after installation, the mobile phone does not need to be adjusted again, which is a visual adjustment and is relatively fast. BRIEF DESCRIPTION OF DRAWINGS

[0039] Figure 1 It is a three-dimensional structure schematic diagram of the present application without installing a mobile phone and inserting a loading carrier;

[0040] Figure 2 It is a three-dimensional structure schematic diagram of the shooting calibration frame in the present application;

[0041] Figure 3 It is Figure 1 It is a three-dimensional structure schematic diagram of the mobile phone positioning support removed;

[0042] Figure 4 It is a three-dimensional structure schematic diagram of the supporting bracket in the present application;

[0043] Figure 5 It is a sectional structure schematic diagram of the present application without installing a mobile phone and inserting a loading carrier;

[0044] Figure 6 It is a three-dimensional structure schematic diagram of one of the loading carriers used in the present application;

[0045] Figure 7 It is a rear view structure schematic diagram of the present application without installing a mobile phone and inserting a loading carrier. DETAILED DESCRIPTION

[0046] The present application will be further described in detail below in combination with the embodiment of the drawings.

[0047] As shown in the figure, a biological macromolecule detection device, comprising a detection base 1, a sample loading positioning mechanism, a sample image capturing mechanism and a light source mechanism are provided on the detection base 1, the biological macromolecule sample to be detected is positioned by the sample loading positioning mechanism, the light source mechanism provides an illumination light source for the biological macromolecule sample to be detected positioned and installed on the sample loading positioning mechanism, and the image information of the biological macromolecule sample to be detected is captured by the sample image capturing mechanism.

[0048] In this embodiment, the sample loading positioning mechanism includes a loading carrier 21 for loading the biological macromolecule sample to be tested and a positioning seat 22 for positioning and mounting the loading carrier 21, the positioning seat 22 has an inner cavity 221, and an image information capturing port (not shown in the figure) penetrating the inner cavity 221 is arranged on the front end face of the positioning seat 22, the positioning seat 22 is provided with a positioning slot 223 for positioning and mounting the loading carrier 21 into the inner cavity 221, and the biological macromolecule sample to be tested is aligned with the image information capturing port by inserting the loading carrier 21 into the positioning seat 22 through the positioning slot 223. The light source mechanism is arranged in the inner cavity 221, and the light source mechanism is arranged before the positioning slot 223 to irradiate the loading carrier 21 from front to back. The structure is simple, the loading carrier 21 is used to load the biological macromolecule sample to be tested, which can be a colloidal gold test strip, a fluorescently labeled chip, etc., the stable positioning and alignment mounting of the loading carrier 21 are realized through the positioning slot 223, and it is ensured that the biological macromolecule sample to be tested can be aligned with the image information capturing port, so that the sample image capturing mechanism can accurately obtain the effective information of the biological macromolecule sample to be tested.

[0049] In this embodiment, a filter 5 is detachably arranged on the image information capturing port. The filter 5 is used for selective light transmission to improve the specificity of detection. It is adapted for use in fluorescence detection scenarios. When the sample to be tested is labeled with fluorescence (such as SYBR Green I), the filter 5 allows specific wavelengths of emitted light to pass through while blocking excitation light and other stray light. For example, the excitation light wavelength is 375 nm (LED light bar emits), and the emission light wavelength is 480 nm (fluorescent substance emits). Then the filter 5 (such as a 480 nm band-pass filter) will block the 375 nm excitation light and only allow the 480 nm fluorescent signal to enter the mobile phone camera, significantly improving the signal-to-noise ratio. It is adapted for use in colorimetric detection scenarios, such as non-fluorescent detection of colloidal gold test strips, etc. The filter 5 can filter environmental light interference (such as ultraviolet or infrared light), ensuring the true restoration of the color development result. The filter 5 is detachably mounted, and different use requirements can quickly realize the replacement of the corresponding filter 5, avoiding the cross contamination or wavelength limitation problem caused by fixed filter, thereby improving the versatility of the device.

[0050] In this embodiment, the filter 5 has a mounting frame 51, the filter 5 is mounted in the mounting frame 51, and the mounting frame 51 is detachably embedded in the image information capturing port. The corresponding area of the filter 5 is the image information capturing area, and the biological macromolecule sample to be tested is aligned with the image information capturing area by inserting the loading carrier 21 into the positioning seat 22 through the positioning slot 223. The mounting frame 51 ensures the accurate positioning of the filter 5, avoids the light leakage or deviation affecting the imaging quality, and the detachable structure facilitates the cleaning or replacement of the damaged filter 5, reducing the maintenance cost.

[0051] In this specific embodiment, a positioning mounting column 6 with an internally threaded hole is arranged in the image information capturing port, and a positioning screw 7 is screwed on the positioning mounting column 6 through a corresponding mounting hole arranged on the mounting frame 51, so as to realize detachable mounting of the filter 5 on the image information capturing port. The structure is simple and convenient to disassemble and assemble.

[0052] In this specific embodiment, the light source mechanism is a multi-wavelength LED light bar 41 arranged in the inner cavity 221. The multi-wavelength light source (such as 375 nm and 480 nm) is integrated in a single light bar, which saves space and supports multi-mode detection (such as fluorescence / ultraviolet). Compared with the traditional single-color light source, the flexibility is higher, and it can be adapted to different markers (such as SYBR Green I and FITC).

[0053] In this specific embodiment, various LED patch lamp beads of different wavelengths are arranged in the LED light bar 41. Different lamp beads can be controlled to emit light by the control circuit according to needs, so as to emit light of fixed wavelength for exciting different fluorescent substances in the biological macromolecule sample to be tested. Programmed control of different lamp beads to emit light realizes automatic wavelength switching and reduces manual intervention errors; precise matching of fluorescent substance excitation requirements (such as 375 nm excitation of SYBR Green I) improves signal-to-noise ratio.

[0054] In this specific embodiment, the sample image capturing mechanism is a mobile phone (not shown in the figure) with shooting function and installed with a matching detection software and a mobile phone positioning support. The mobile phone positioning support includes a shooting calibration frame 321 for aligning the camera of the mobile phone (not shown in the figure) with the image information capturing port and a supporting bracket 322 for supporting the bottom of the mobile phone (not shown in the figure). The shooting calibration frame 321 is detachably arranged on the positioning seat 22, and the supporting bracket 322 is adjustably arranged on the detection base 1. The mobile phone camera and computing resources are used to reduce hardware cost and be compatible with existing smart devices; the support design ensures that the camera is aligned with the sample, avoiding image blur or angle deviation caused by handheld shooting.

[0055] In this specific embodiment, the shooting calibration frame 321 has a through cavity 323 passing through front and back, the left frame fixing piece 324 and the right frame fixing piece 325 are slidably arranged in the through cavity 323, the image capture hole 326 is formed between the left frame fixing piece 324 and the right frame fixing piece 325, the front end face of the positioning seat 22 is protruded and provided with the left limiting tab 327 for limiting the left frame fixing piece 324 and the right limiting tab 328 for limiting the right frame fixing piece 325, the image information capture hole is located between the left limiting tab 327 and the right limiting tab 328, the front end face of the positioning seat 22 is protruded and provided with the upper limiting plate 329 for forming upper limiting installation of the shooting calibration frame 321 and the lower limiting plate 330 for forming lower limiting installation of the shooting calibration frame 321; after installation, the shooting calibration frame 321 is embedded between the upper limiting plate 329 and the lower limiting plate 330, the left frame fixing piece 324 is attached to the outer side of the left limiting tab 327, and the right frame fixing piece 325 is attached to the outer side of the right limiting tab 328. The slidable left / right frame fixing piece is suitable for different mobile phone camera positions, ensuring that the capture area completely coincides with the sample detection area; the limiting tab provides physical stop to prevent excessive movement of the frame and affect the calibration accuracy.

[0056] In this specific embodiment, the middle part of the left frame fixing piece 324 and the right frame fixing piece 325 is respectively protruded outward to form an arc-shaped protruding part 331, the two protruding parts 331 are mirror-symmetrically arranged, and the left limiting tab 327 and the right limiting tab 328 are arc-shaped tab bodies matched with each other. The arc-shaped structure fits the mobile phone camera shape, enhances stability and avoids scratching the equipment; the mirror-symmetric design simplifies the operation process and makes the left and right adjustment logic consistent.

[0057] In this specific embodiment, the upper inner end face and the lower inner end face of the through cavity 323 are respectively provided with a sliding groove 332 in the left-right direction, the upper and lower ends of the left frame fixing piece 324 and the right frame fixing piece 325 are respectively slidably installed in the corresponding sliding grooves 332, and at least one sliding groove 332 is detachably provided with a locking buckle 333 respectively used for locking the sliding position of the left frame fixing piece 324 and the right frame fixing piece 325; after installation, the inner and outer sides of the left frame fixing piece 324 are respectively limited by the corresponding locking buckle 333 and the left limiting tab 327, and the inner and outer sides of the right frame fixing piece 325 are respectively limited by the corresponding locking buckle 333 and the right limiting tab 328. The sliding groove 332 realizes stepless adjustment, the locking buckle 333 quickly fixes, and flexibility and reliability are considered; accidental sliding of the frame fixing piece during detection is prevented, and image acquisition consistency is ensured.

[0058] In this specific embodiment, the shooting calibration frame 321 is composed of two opposite left and right movable clamping arms 334, and an elastic reset element 335 is arranged between the two clamping arms 334, and a clamping cavity for clamping a mobile phone (not shown in the figure) is formed between the two clamping arms 334. Through the elastic reset element, the width of different mobile phones is automatically adapted, and manual adjustment of the clamping force is not required; the clamping cavity is designed to be compatible with the size of mainstream mobile phones, thereby improving the user experience.

[0059] In this specific embodiment, the elastic reset element is a reset spring. The structure is simple, the cost is low, and the use is stable.

[0060] In this specific embodiment, a guide rod 8 is arranged on the detection base 1, and a matched guide hole 9 is arranged on the supporting bracket 322. The guide hole 9 and the guide rod 8 are matched to realize the adjustment and installation of the up-down height position of the supporting bracket 322 on the detection base 1, and a locking mechanism for locking the position of the supporting bracket 322 is arranged between the supporting bracket 322 and the detection base 1. The guide rod 8 and the guide hole 9 are matched to realize precise up-down adjustment and movement, and ensure the best focusing distance of the mobile phone camera and the sample plane.

[0061] In this specific embodiment, the locking mechanism includes a locking bolt 101 and a locking plate 102. The locking plate 102 is arranged in the detection base 1, and a sliding groove 103 for sliding installation of the locking plate 102 is arranged in the detection base 1 along the activity direction of the supporting bracket 322. The locking plate 102 is slidably arranged in the sliding groove 103 synchronously with the supporting bracket 322. The locking plate 102 is provided with a locking screw hole (not shown in the figure) matched with the locking bolt 101. The front end face of the detection base 1 is provided with a guide groove 104 communicated with the sliding groove 103 along the activity direction of the supporting bracket 322. The locking bolt 101 is arranged on the supporting bracket 322, and the rod part of the locking bolt 101 is screwed on the locking plate 102 through the guide groove 104. In the locked state, the locking bolt 101 is in the tightened state, so that the head part of the locking bolt 101 is pressed on the front end face of the supporting bracket 322, and the rear end face of the supporting bracket 322 is arranged on the detection base 1. The above-mentioned locking mechanism has simple structure and convenient operation. The sliding groove 103 provides a stable installation position for the locking plate 102, and also plays the role of active guide.

[0062] In this specific embodiment, the front part of the detection base 1 is recessed to form a recess 11, and the guide rod 8 is arranged in the recess 11. The space of the detection base 1 is reasonably utilized, the recess 11 hides the guide rod 8, reduces the interference of external structure, and improves the overall appearance and safety of the equipment.

[0063] In this specific embodiment, the positioning seat 22 is obliquely supported on the detection base 1, and the guide rod 8 is obliquely arranged on the detection base 1, and the inclination angle of the positioning seat 22 is the same as that of the guide rod 8. The uniform inclination angle (such as 60°) optimizes the user's visual angle, reduces the interference of reflection, and facilitates operation; the logical and consistent structure design reduces the assembly complexity.

[0064] In this specific embodiment, a rechargeable battery 12 is arranged in the detection base 1, and a matching charging interface 13 is arranged on the detection base 1. The external power supply is eliminated, and continuous use in the field or mobile scene is supported; the charging interface 13 is standardized (such as Type-C) and compatible with common charging equipment.

[0065] In this specific embodiment, a light control circuit is arranged in the detection base 1, and a light control switch 15 electrically connected to the light control circuit is arranged on the detection base 1. The independent switch controls the LED light bar 41, avoids accidental touch or standby power consumption, and prolongs the battery life; the mode switching function (such as constant light / pulse light source) can be integrated, and different detection protocols can be adapted.

[0066] The specific operation process of the device is as follows:

[0067] (1) Device assembly:

[0068] Place the detection base 1 on a stable table top to ensure that the light source mechanism (LED light bar 41) and the positioning seat 22 are stably connected;

[0069] If the filter 5 needs to be used, select the appropriate filter 5 (suitable for different fluorescence detection requirements), align the mounting frame 51 of the filter 5 with the image information capture port, and fix it with the positioning screw 7 to install the filter 5 into the image information capture port;

[0070] (2) Mobile phone installation:

[0071] Place the mobile phone with the matching detection software installed on the mobile phone positioning bracket, adjust the clamping arm 334 to clamp the mobile phone, adjust the height of the supporting bracket 322 (through the guide rod 8 and the locking bolt 101), and at the same time, adapt the camera position of different mobile phones by sliding the left / right frame fixing piece, and after the adjustment is completed, use the locking buckle 333 to fix the position, to ensure that the camera is aligned with the image information capture port;

[0072] (3) Sample loading:

[0073] Place the biological macromolecule sample to be tested into the matching loading carrier 21 (such as colloidal gold test strip, fluorescent label chip, etc.), and insert the loading carrier 21 into the matching positioning slot 223 to ensure that the sample detection area is opposite to the image information capture port;

[0074] If the sample needs fluorescence excitation, select the corresponding wavelength LED lamp bead (such as 375nm excites SYBR Green I) through the light control switch;

[0075] (4) Detection process:

[0076] Start the detection software (pre-installed on the mobile phone), adjust the camera parameters (such as exposure, white balance);

[0077] Turn on the light source, and the LED light bar irradiates the sample to excite the fluorescence signal (if applicable);

[0078] Click the image capture button in the software to take a high-definition image of the sample detection area;

[0079] (5) Data analysis:

[0080] The detection software automatically analyzes the image:

[0081] For colloidal gold test strips, identify the color intensity of T / C lines and calculate the concentration ratio;

[0082] For fluorescence-labeled samples, analyze the fluorescence signal intensity and generate a quantitative report;

[0083] The software can save the detection results and support historical data comparison or export.

[0084] (6) Equipment maintenance:

[0085] After the detection is completed, remove the sample, clean the positioning slot 223 and the filter 5 (to avoid contamination);

[0086] Turn off the LED light source and disconnect the power supply (if not used for a long time);

[0087] Regularly check the battery 12 and supplement the charge through the charging interface 13.

Claims

1. A biomacromolecule detection device, comprising a detection base, wherein the detection base is provided with a sample loading and positioning mechanism, a sample image capture mechanism, and a light source mechanism. A sample to be detected is positioned by the sample loading and positioning mechanism, the light source mechanism provides an illumination source for positioning the sample mounted on the sample loading and positioning mechanism, and the sample image capture mechanism captures image information of the sample; characterized in that: The sample loading and positioning mechanism includes a loading carrier and a positioning seat, the positioning seat having an inner cavity and an image information capture port provided at the front end and communicating with the inner cavity, the loading carrier is inserted into the inner cavity so that the sample is aligned with the image information capture port, and the light source mechanism is provided in the inner cavity and in front of the loading carrier; The sample image capture mechanism includes a mobile phone with detection software and a mobile phone positioning bracket, the mobile phone positioning bracket includes a shooting calibration frame detachably mounted on the positioning seat, the shooting calibration frame having a through cavity, and left and right framing pieces slidably mounted in the through cavity to form an adjustable image capture hole; The positioning seat is provided with a limiting mechanism for limiting the left and right frame pieces so that the image capture hole is aligned with the image information capture port; The limiting mechanism includes a left limiting protrusion protruding from the front end surface of the positioning seat for limiting the left framing piece and a right limiting protrusion for limiting the right framing piece. The image information capture port is located between the left limiting protrusion and the right limiting protrusion. The front end surface of the positioning seat is protruding with an upper limit plate for forming an upper limit installation for the shooting calibration frame and a lower limit plate for forming a lower limit installation for the shooting calibration frame. After the installation is completed, the shooting calibration frame is embedded between the upper limit plate and the lower limit plate, the left framing piece is attached to the outer side of the left limiting protrusion, and the right framing piece is attached to the outer side of the right limiting protrusion.

2. A biomacromolecule detection device according to claim 1, characterized in that The positioning seat is provided with a positioning slot for the loading carrier to be positioned and installed in the inner cavity. The loading carrier is inserted into the positioning seat through the positioning slot so that the sample is aligned with the image information capture port.

3. A biomacromolecule detection device as claimed in claim 2, characterized in that The image information capturing port is detachably provided with a filter.

4. A biomacromolecule detection device as claimed in claim 3, characterized in that The filter has a mounting frame, the filter is mounted in the mounting frame, the mounting frame is detachably embedded in the image information capture port, the area corresponding to the filter is the image information capture area, and the loading carrier is inserted into the positioning seat through the positioning slot so that the biological macromolecule sample to be detected is aligned with the image information capture area.

5. A biomacromolecule detection device according to claim 1, characterized in that The light source mechanism is a multi-wavelength LED light bar.

6. A biomacromolecule detection device according to claim 1, characterized in that The mobile phone positioning bracket also includes a supporting bracket. The shooting calibration frame is used to align the camera of the mobile phone with the image information capture port. The supporting bracket is used to support the bottom of the mobile phone. The supporting bracket can be adjusted up and down on the detection base.

7. A biomacromolecule detection device according to claim 1, characterized in that The upper inner end surface and the lower inner end surface of the through cavity are respectively provided with sliding grooves in the left and right directions, and the upper and lower ends of the left framing piece and the right framing piece are respectively slidably installed in the corresponding sliding grooves, and at least one of the sliding grooves is detachably provided with a locking buckle for locking the sliding position of the left framing piece and the right framing piece respectively; After installation is completed, the inner and outer sides of the left frame fixing piece are respectively limited by the corresponding locking buckles and the left limiting protrusion, and the inner and outer sides of the right frame fixing piece are respectively limited by the corresponding locking buckles and the right limiting protrusion.

8. A biomacromolecule detection device according to claim 6, characterized in that The detection base is provided with a guide rod, and the support bracket is provided with a corresponding guide hole. The upper and lower height positions of the support bracket on the detection base can be adjusted and installed through the cooperation between the guide hole and the guide rod. A locking mechanism for locking the position of the support bracket is provided between the support bracket and the detection base.

9. A biomacromolecule detection device according to claim 8, characterized in that The locking mechanism comprises a locking bolt and a locking plate, wherein the locking plate is arranged in the detection base, and a sliding groove is provided in the detection base along the movable direction of the support bracket for sliding installation of the locking plate. The locking plate can be slidingly arranged in the sliding groove along the synchronous movement of the support bracket, and the locking plate is provided with a locking screw hole that cooperates with the locking bolt, and the front end surface of the detection base is provided with a guide groove connected to the sliding groove along the movable direction of the support bracket, and the locking bolt is arranged on the support bracket, and the rod of the locking bolt passes through the guide groove and is screwed to the locking plate; in the locked state, the locking bolt is in a tightened state, so that the head of the locking bolt is pressed against the front end surface of the support bracket, so that the rear end surface of the support bracket is abutted against the detection base.

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