Mycotoxin and heavy metal two-in-one fully automatic rapid detector

Abstract

The present invention discloses a two-in-one fully automatic rapid detector for mycotoxins and heavy metals. The detector comprises a base, a cabinet, a loading and unloading conveyor belt, a handling module, a pipetting mechanism, a detection platform, a stirring mechanism and a control panel. The pipetting mechanism comprises a three-axis displacement module and a cleaning tank. The detection platform comprises a base frame. The stirring mechanism comprises a two-axis displacement module and a second cylinder. The cabinet, the loading and unloading conveyor belt, the three-axis displacement module, the cleaning tank, the base frame and the two-axis displacement module are all fixedly connected to the base. The control panel is fixedly connected to the cabinet. The control panel is connected to the three-axis displacement module and the second cylinder via electrical signals. The present invention relates to the technical field of automated detection equipment. The present invention simultaneously detects mycotoxins and heavy metals lead and cadmium that may cause harm in grain. The detection is performed by switching between two methods, time-resolved fluorescence immunochromatography and colloidal gold immunochromatography. The process from sample pre-treatment to detection results is fully automatic and integrated.

Description

Technical Field

[0001] The present invention relates to the technical field of automated detection equipment, in particular to a two-in-one fully automatic rapid detection instrument for mycotoxins and heavy metals. Background Art

[0002] Mycotoxin and heavy metal pollution are among the main threats to food security. We will overcome bottlenecks in the automated extraction of mycotoxins from grains, integrate key technologies such as automated pretreatment and chromatographic immunoassay, develop integrated automated detection equipment that integrates sample extraction, separation, and sample transfer, and establish an automated intelligent detection platform for mycotoxins and heavy metals in grains to meet the needs of diverse applications.

[0003] The construction of this platform realizes the full-process automated quantitative detection of mycotoxins and heavy metal pollution with the principle of "sample in, result out". Only one extraction and one-click operation are required to obtain the detection results of mycotoxins and heavy metal pollution in the sample, which greatly shortens the detection time and workload, reduces personnel requirements and intervention, improves the efficiency and accuracy of mycotoxin and heavy metal detection in grassroots grains, and reduces detection costs. When automatically detecting grain sample solutions, it is necessary to control the interference of control variables on the test results. For example, sample residues from multiple pipetting on the pipette will cause sample solutions of different concentrations to mix, which interferes with the detection accuracy. For sample solutions of different concentrations, different degrees of precipitation will cause deviations in sampling accuracy, and the solution needs to be pretreated and stirred. Summary of the Invention

[0004] The purpose of the present invention is to provide a two-in-one fully automatic rapid detector for mycotoxins and heavy metals to solve the problems in the prior art.

[0005] To achieve the above-mentioned objectives, the present invention provides the following technical solutions: a two-in-one fully automatic rapid detector for mycotoxins and heavy metals includes a base, a cabinet, a loading and unloading conveyor belt, a handling module, a pipetting mechanism, a detection platform, a stirring mechanism and a control panel. The pipetting mechanism includes a three-axis displacement module and a cleaning tank. The detection platform includes a base frame. The stirring mechanism includes a two-axis displacement module and a second cylinder. The cabinet, the loading and unloading conveyor belt, the three-axis displacement module, the cleaning tank, the base frame and the two-axis displacement module are all fixedly connected to the base. The control panel is fixedly connected to the cabinet. The control panel is connected to the three-axis displacement module and the second cylinder through electrical signals.

[0006] The present invention relates to a fully automatic detector for extracting a grain sample solution for rapid testing. Pre-crushed grain sample powder is placed in a sample cup, which is transported into a cabinet by a loading and unloading conveyor belt and automatically weighed within a specified weight range. An ethanol solution in a specified proportion is added into the sample cup, and the grain powder and the ethanol solution in the sample cup are stirred for a fixed time. The mixture is then allowed to settle and filtered to absorb the supernatant, and then a sample diluent is added according to a specified proportion. A control panel sends an electrical signal to a second cylinder, which drives a stirring mechanism to adjust the stirring amplitude. A transport module transports the sample cup to a testing station, a pipetting mechanism is first cleaned with clean water in a cleaning tank, and then the sample solution in the sample cup is extracted and dropped onto a testing platform. Time-resolved fluorescence immunochromatography and colloidal gold immunochromatography are used to detect mycotoxins and heavy metals in the grain sample solution, respectively.

[0007] Furthermore, the pipetting mechanism also includes a sampling mechanism, and the sampling mechanism includes a side plate, and the side plate is fixedly connected to the three-axis displacement module.

[0008] The sampling mechanism is assembled on the three-axis displacement module through the side plate. Before pipetting, the three-axis displacement module drives the sampling mechanism, places the pipette gun in the cleaning tank with clean water to clean the residue from the last pipetting, and the flipping mechanism drives the pipette gun to flip to the self-cleaning mechanism. After the sampling mechanism is self-cleaned, the pipetting operation is performed.

[0009] Furthermore, the sampling mechanism also includes a flipping mechanism, a self-cleaning mechanism and a pipette gun. The flipping mechanism includes a rotary spring seat, a servo cylinder and a rotating column. The self-cleaning mechanism includes a side frame. The rotary spring seat, the servo cylinder and the side frame are all fixedly connected to the side plate, and the rotating column is fixedly connected to the pipette gun.

[0010] The pipette is placed in a cleaning tank with clean water to clean the residue from the last pipetting. The output end of the servo cylinder moves, driving the pipette to flip to the self-cleaning mechanism. The needle of the pipette is placed in the self-cleaning mechanism to remove the residual liquid on the outer surface of the needle.

[0011] Furthermore, the flipping mechanism also includes a knife-shaped plate and a slide, the knife-shaped plate is rotatably connected to the rotary spring seat, the slide is fixedly connected to the output end of the servo cylinder, the slide is in contact with the knife-shaped plate, a wave groove is provided on the knife-shaped plate, a circular frame is provided on the slide, the rotating column is rotatably connected to the wave groove, and the rotating column is in contact with the circular frame.

[0012] The output end of the servo cylinder initially displaces away from the rotary spring seat, the pipette head downward, and the output end of the servo cylinder displaces upward, and the rotating column is pulled in the wave groove by the round frame on the slide, and displaced toward one end of the rotary spring seat and rotated synchronously. When the rotating column displaces in the wave groove, when it passes the crest position, the crest position is directly below the rotary spring seat, that is, the knife-shaped plate rotates away from the servo cylinder with the rotary spring seat as the center of the circle, and then the knife-shaped plate is reset under the action of the rotary spring seat. When the output end of the servo cylinder displaces close to the rotary spring seat, the rotating column is located in the wave groove close to one end of the rotary spring seat. At this time, the rotating column is fixedly assembled with the pipette, and the pipette head is upwardly located in the self-cleaning mechanism.

[0013] Furthermore, the self-cleaning mechanism also includes a box body, a spring slider, a column buckle, a servo motor, a cleaning column, a first cylinder and a slide plate. The box body is provided with side slide grooves and convex columns. The side slide grooves, spring slider, column buckle and cleaning column are each provided with two groups. The spring slider is slidingly connected to the side slide groove, and the column buckle is fixedly connected to the spring slider and the servo motor. A gear disk is provided on the cleaning column, and the spring slider is rotatably connected to the gear disk. A first through hole is provided on the spring slider, and a second through hole is provided on the column buckle. The output end of the servo motor is in contact with the first through hole and the second through hole. The output end of the servo motor is rotatably connected to the cleaning column, the first cylinder is fixedly connected to the box body, and the output end of the first cylinder is fixedly connected to the slide plate. The slide plate is provided with an inclined slide groove and a straight groove, the column buckle is slidably connected to the inclined slide groove, and the convex column is slidably connected to the straight groove.

[0014] The rotating column is located in the wave groove near one end of the rotary spring seat, and the pipette tip is upwardly located between the two groups of cleaning columns. In the initial state, the slide is located near one end of the first cylinder. At this time, the spring slider is located in the side slide groove away from the end of the convex column. Then the output end of the first cylinder pushes the slide away, the convex column and the linear groove are relatively displaced, and the two groups of column buckles are displaced toward each other along the oblique slide groove. Under the action of the elastic force of the spring slider itself to restore the compression, the two groups of cleaning columns are displaced toward each other and close to the pipette tip, and the tooth surfaces of the two groups of gear discs are engaged. The servo motor outputs a fixed-axis torque to the cleaning column, and the two groups of cleaning columns rotate synchronously in the opposite direction of the torque to self-clean the surface of the pipette tip.

[0015] Furthermore, the detection platform also includes a fluorescence detection platform and a colloidal gold detection platform, and the fluorescence detection platform and the colloidal gold detection platform are both fixedly connected to the base frame.

[0016] The grain sample solution was dropped onto the fluorescence detection platform and the colloidal gold detection platform using a pipette, and the fungal toxins and heavy metals in the grain sample solution were detected by time-resolved fluorescence immunochromatography and colloidal gold immunochromatography, respectively.

[0017] Furthermore, the stirring mechanism also includes a driving motor, an angle adjustment mechanism and a stirring rod. The angle adjustment mechanism includes a cylinder, a curved body and a square frame. The driving motor is fixedly connected to the two-axis displacement module. The second cylinder is fixedly connected to the output end of the driving motor and the cylinder. The output end of the second cylinder is fixedly connected to the square frame. A lower protrusion is provided on the curved body, and the lower protrusion is fixedly connected to the stirring rod.

[0018] After the two-axis displacement module moves the drive motor to the top of the sample cup, the drive motor moves downward. The output end of the second cylinder pushes the frame back and forth, continuously adjusting the inclination angle of the stirring rod to continuously change the stirring amplitude of the stirring rod. The drive motor outputs a fixed-axis torque to drive the fixed-axis rotation of the second cylinder. The second cylinder drives the stirring rod to stir and precipitate the grain powder and ethanol solution in the sample cup for pre-treatment.

[0019] Furthermore, the angle adjustment mechanism also includes a hemispherical shell and a first gear. The hemispherical shell is fixedly connected to the cylinder, an upper protrusion is provided on the arc surface body, a first arc groove is provided on the cylinder, and a second arc groove is provided on the hemispherical shell. The upper protrusion is slidably connected to the first arc groove, the lower protrusion is slidably connected to the second arc groove, the first gear is fixedly connected to the upper protrusion, an internal tooth pair is provided on the square frame, and the first gear is engaged with the tooth surface of the internal tooth pair.

[0020] The output end of the second cylinder pushes the frame to move. Through the engagement between the internal tooth pair of the frame and the tooth surface of the first gear, the vertical displacement of the frame is converted into the arc surface body rotating between the cylinder and the hemispherical shell with the first gear as the center. The upper protrusion slides in the first arc groove, and the lower protrusion slides in the second arc groove. The lower protrusion drives the stirring rod to adjust the inclination angle, and continuously adjusts the inclination angle of the stirring rod to continuously change the stirring amplitude of the stirring rod, ensuring that the grain powder and ethanol solution are completely stirred and mixed evenly, avoiding sedimentation causing sample sampling errors.

[0021] Compared with the prior art, the beneficial effects of the present invention are as follows: the present invention designs a self-cleaning mechanism, the pipette tip is cleaned with clean water in the cleaning tank, the servo cylinder output end initially displaces away from the rotary spring seat, the pipette tip moves downward, the servo cylinder output end moves upward, and the rotating column is pulled by the circular frame on the slide, and the rotating column moves toward one end of the rotary spring seat in the wave groove and rotates synchronously, the rotating column is located in the wave groove close to one end of the rotary spring seat, the pipette tip is upwardly located between the two groups of cleaning columns, the two groups of cleaning columns are displaced toward each other and close to the pipette tip, the servo motor outputs a fixed-axis torque to the cleaning column, and the two groups of cleaning columns rotate synchronously in the same direction in the opposite direction of the torque, and the surface of the pipette tip is self-cleaned, the pipette repeatedly draws clean water to clean the inside, and before pipetting, the self-cleaning mechanism quickly cleans the residue on the surface of the tip, eliminates the interference of the residue of the last pipetting on the result of this pipetting, and improves the pipetting accuracy; the present invention designs an angle adjustment mechanism, the second cylinder output end pushes The movable frame is displaced, and the tooth surface between the internal gear pair and the first gear is meshed. The arcuate body rotates between the cylinder and the hemispherical shell with the first gear as the center, driving the stirring rod to adjust the inclination angle. The driving motor outputs a fixed-axis torque to drive the fixed-axis rotation of the second cylinder, so that the stirring amplitude of the stirring rod is continuously changed, ensuring that the grain powder and the ethanol solution are completely stirred and mixed evenly, and avoiding sample sampling errors caused by sediments; the present invention designs a detection platform, and drops the grain sample solution on the fluorescence detection table and the colloidal gold detection table, and respectively detects the fungal toxins and heavy metals in the grain sample solution by time-resolved fluorescence immunochromatography and colloidal gold immunochromatography; the present invention simultaneously detects the fungal toxins and heavy metal lead and heavy metal cadmium that may cause harm in grain, and performs detection by switching the two methods of time-resolved fluorescence immunochromatography and colloidal gold immunochromatography. The equipment involves sample pretreatment, sample detection and results, and is fully automatic and integrated. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0023] Figure 2 Schematic diagram of the structure of the liquid transfer mechanism of the present invention;

[0024] Figure 3 It is a schematic structural diagram of the sampling mechanism of the present invention;

[0025] Figure 4 It is a schematic structural diagram of the turning mechanism of the present invention;

[0026] Figure 5 It is a structural schematic diagram of the self-cleaning mechanism of the present invention;

[0027] Figure 6 Schematic diagram of the stirring mechanism structure of the present invention;

[0028] Figure 7 Schematic diagram of the detection platform structure of the present invention;

[0029] Figure 8 Schematic diagram of the angle adjustment mechanism structure of the present invention;

[0030] Figure 9 for Figure 5 A magnified schematic diagram of local A.

[0031] In the figure: 1. Base; 2. Cabinet; 3. Loading and unloading conveyor belt; 4. Handling module; 5. Liquid transfer mechanism; 51. Three-axis displacement module; 52. Cleaning tank; 53. Sampling mechanism; 54. Side panel; 55. Turning mechanism; 551. Rotary spring seat; 552. Blade plate; 5521. Wave groove; 553. Servo cylinder; 554. Slide; 5541. Round frame; 555. Rotating column; 56. Self-cleaning mechanism; 561. Side frame; 562. Box; 5621. Side slide groove; 5622. Boss; 563. Spring slider; 5631. First through hole; 564. Column buckle; 5641. Second through hole; 565. Servo motor; 566. Cleaning column ;5661, gear disc;567, first cylinder;568, slide plate;5681, inclined slide;5682, linear groove;57, pipette;6, detection platform;61, base frame;62, fluorescence detection platform;63, colloidal gold detection platform;7, stirring mechanism;71, two-axis displacement module;72, drive motor;73, second cylinder;74, angle adjustment mechanism;741, cylinder;7411, first arc groove;742, hemispherical shell;7421, second arc groove;743, arc surface;7431, upper protrusion;7432, lower protrusion;744, first gear;745, frame;7451, internal gear pair;75, stirring rod;8, control panel. DETAILED DESCRIPTION

[0032] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0033] like Figure 1 、 Figure 2 、 Figure 7As shown, the present invention provides a technical solution of a two-in-one fully automatic rapid detector for mycotoxins and heavy metals, including a base 1, a cabinet 2, a loading and unloading conveyor belt 3, a transport module 4, a pipetting mechanism 5, a detection platform 6, a stirring mechanism 7 and a control panel 8. The pipetting mechanism 5 includes a three-axis displacement module 51 and a cleaning tank 52, the detection platform 6 includes a base frame 61, the stirring mechanism 7 includes a two-axis displacement module 71 and a second cylinder 73, the cabinet 2, the loading and unloading conveyor belt 3, the three-axis displacement module 51, the cleaning tank 52, the base frame 61, and the two-axis displacement module 71 are all fixedly connected to the base 1, the control panel 8 is fixedly connected to the cabinet 2, and the control panel 8 is connected to the three-axis displacement module 51 and the second cylinder 73 through electrical signals.

[0034] The present invention is a fully automatic detector for extracting grain sample solutions for rapid testing. Pre-crushed grain sample powder is placed in a sample cup, which is transported to a cabinet 2 via a loading and unloading conveyor belt 3 and automatically weighed to within a specified weight range. An ethanol solution in a specified proportion is added to the sample cup, and the grain powder and the ethanol solution in the sample cup are stirred for a fixed time. The mixture is then allowed to settle and filtered to absorb the supernatant, and then a sample diluent is added according to a specified proportion. A control panel 8 sends an electrical signal to a second cylinder 73, which drives a stirring mechanism 7 to adjust the stirring amplitude. A transport module 4 transports the sample cup to a testing station. A pipetting mechanism 5 is first cleaned with clean water in a cleaning tank 52, and then the sample solution in the sample cup is extracted and dropped onto a testing platform 6. Time-resolved fluorescence immunochromatography and colloidal gold immunochromatography are used to detect mycotoxins and heavy metals in the grain sample solution, respectively.

[0035] like Figure 2 As shown, the pipetting mechanism 5 further includes a sampling mechanism 53 . The sampling mechanism 53 includes a side plate 54 . The side plate 54 is fixedly connected to the three-axis displacement module 51 .

[0036] The sampling mechanism 53 is assembled on the three-axis displacement module 51 through the side plate 54. Before pipetting, the three-axis displacement module 51 drives the sampling mechanism 53, places the pipette gun 57 in the cleaning tank 52 with clean water to clean the residue from the last pipetting, and the flipping mechanism 55 drives the pipette gun 57 to flip to the self-cleaning mechanism 56. The sampling mechanism 53 performs pipetting operations after self-cleaning.

[0037] like Figure 3 、 Figure 4 、 Figure 5 As shown, the sampling mechanism 53 also includes a flipping mechanism 55, a self-cleaning mechanism 56 and a pipette gun 57. The flipping mechanism 55 includes a rotary spring seat 551, a servo cylinder 553 and a rotating column 555. The self-cleaning mechanism 56 includes a side frame 561. The rotary spring seat 551, the servo cylinder 553 and the side frame 561 are all fixedly connected to the side plate 54. The rotating column 555 is fixedly connected to the pipette gun 57.

[0038] The pipette gun 57 is placed in the cleaning tank 52 with clean water to clean the residue from the last pipetting. The output end of the servo cylinder 553 moves, driving the pipette gun 57 to flip to the self-cleaning mechanism 56, and the needle tube of the pipette gun 57 is placed in the self-cleaning mechanism 56 to remove the residual liquid on the outer surface of the needle tube.

[0039] like Figure 3 、 Figure 4 、 Figure 5 As shown, the flipping mechanism 55 also includes a knife-shaped plate 552 and a slide 554. The knife-shaped plate 552 is rotatably connected to the rotary spring seat 551. The slide 554 is fixedly connected to the output end of the servo cylinder 553. The slide 554 is in contact with the knife-shaped plate 552. A wave groove 5521 is provided on the knife-shaped plate 552. A circular frame 5541 is provided on the slide 554. The rotating column 555 is rotatably connected to the wave groove 5521. The rotating column 555 is in contact with the circular frame 5541.

[0040] The output end of the servo cylinder 553 initially moves away from the rotary spring seat 551, and the tip of the pipette gun 57 moves downward. The output end of the servo cylinder 553 moves upward, and the round frame 5541 on the slide 554 pulls the rotating column 555 in the wave groove 5521, and moves toward one end of the rotary spring seat 551 and rotates synchronously. When the rotating column 555 moves in the wave groove 5521, when it passes the peak position, the peak position is directly below the rotary spring seat 551, that is, the knife-shaped plate 552 rotates away from the servo cylinder 553 with the rotary spring seat 551 as the center of the circle. Then, under the action of the rotary spring seat 551, the knife-shaped plate 552 is reset. When the output end of the servo cylinder 553 moves close to the rotary spring seat 551, the rotating column 555 is located in the wave groove 5521 near one end of the rotary spring seat 551. At this time, the rotating column 555 is fixedly assembled with the pipette gun 57, and the tip of the pipette gun 57 is upwardly located in the self-cleaning mechanism 56.

[0041] like Figure 3 、 Figure 4 、 Figure 5As shown, the self-cleaning mechanism 56 also includes a box body 562, a spring slider 563, a column buckle 564, a servo motor 565, a cleaning column 566, a first cylinder 567 and a slide plate 568. The box body 562 is provided with a side slide groove 5621 and a convex column 5622. The side slide groove 5621, the spring slider 563, the column buckle 564 and the cleaning column 566 are each provided with two groups. The spring slider 563 is slidably connected to the side slide groove 5621. The column buckle 564 is fixedly connected to the spring slider 563 and the servo motor 565. The cleaning column 566 is provided with a toothed disc 5661. The spring slider 563 is connected to the toothed disc 566. 1 is rotatably connected. A first through hole 5631 is provided on the spring slider 563, and a second through hole 5641 is provided on the column buckle 564. The output end of the servo motor 565 contacts both the first through hole 5631 and the second through hole 5641. The output end of the servo motor 565 is rotatably connected to the cleaning column 566. The first cylinder 567 is fixedly connected to the box body 562. The output end of the first cylinder 567 is fixedly connected to the slide plate 568. The slide plate 568 is provided with an inclined groove 5681 and a linear groove 5682. The column buckle 564 is slidably connected to the inclined groove 5681, and the boss 5622 is slidably connected to the linear groove 5682.

[0042] The rotating column 555 is located in the wave groove 5521 near one end of the rotary spring seat 551, and the tip of the pipette 57 is upwardly located between the two groups of cleaning columns 566. In the initial state, the slide plate 568 is located near one end of the first cylinder 567. At this time, the spring slider 563 is located in the side slide groove 5621 away from the end of the protruding column 5622. Then the output end of the first cylinder 567 pushes the slide plate 568 away, and the protruding column 5622 and the straight groove 5682 are relatively displaced. The two groups of column buckles 564 move toward each other along the inclined slide groove 5681. Under the action of the elastic force of the spring slider 563 itself to restore the compression, the two groups of cleaning columns 566 move toward each other and close to the tip of the pipette 57. The tooth surfaces of the two groups of gear plates 5661 are engaged, and the servo motor 565 outputs a fixed-axis torque to the cleaning columns 566. The two groups of cleaning columns 566 rotate synchronously with the same torque in the opposite direction to self-clean the surface of the tip of the pipette 57.

[0043] like Figure 6 As shown, the detection platform 6 further includes a fluorescence detection platform 62 and a colloidal gold detection platform 63 . Both the fluorescence detection platform 62 and the colloidal gold detection platform 63 are fixedly connected to the base frame 61 .

[0044] The pipette 57 drops the grain sample solution onto the fluorescence detection platform 62 and the colloidal gold detection platform 63 respectively, and detects the fungal toxins and heavy metals in the grain sample solution by time-resolved fluorescence immunochromatography and colloidal gold immunochromatography respectively.

[0045] like Figure 7 、 Figure 8As shown, the stirring mechanism 7 also includes a driving motor 72, an angle adjustment mechanism 74 and a stirring rod 75. The angle adjustment mechanism 74 includes a cylinder 741, a curved body 743 and a square frame 745. The driving motor 72 is fixedly connected to the two-axis displacement module 71. The second cylinder 73 is fixedly connected to the output end of the driving motor 72 and the cylinder 741. The output end of the second cylinder 73 is fixedly connected to the square frame 745. A lower protrusion 7432 is provided on the curved body 743, and the lower protrusion 7432 is fixedly connected to the stirring rod 75.

[0046] After the two-axis displacement module 71 moves the drive motor 72 to above the sample cup, the drive motor 72 moves downward, and the output end of the second cylinder 73 pushes the box 745 back and forth, continuously adjusting the inclination angle of the stirring rod 75, so that the stirring amplitude of the stirring rod 75 continuously changes. The drive motor 72 outputs a fixed-axis torque to drive the fixed-axis rotation of the second cylinder 73. The second cylinder 73 drives the stirring rod 75 to stir and precipitate the grain powder and ethanol solution in the sample cup for pre-treatment.

[0047] like Figure 7 、 Figure 8 As shown, the angle adjustment mechanism 74 also includes a hemispherical shell 742 and a first gear 744. The hemispherical shell 742 is fixedly connected to the cylinder 741. The arc surface body 743 is also provided with an upper protrusion 7431. The cylinder 741 is provided with a first arc groove 7411. The hemispherical shell 742 is provided with a second arc groove 7421. The upper protrusion 7431 is slidably connected to the first arc groove 7411. The lower protrusion 7432 is slidably connected to the second arc groove 7421. The first gear 744 is fixedly connected to the upper protrusion 7431. The square frame 745 is provided with an internal gear pair 7451. The first gear 744 is engaged with the tooth surface of the internal gear pair 7451.

[0048] The output end of the second cylinder 73 pushes the box 745 to move, and through the engagement between the internal gear pair 7451 of the box 745 and the tooth surface of the first gear 744, the vertical displacement of the box 745 is converted into, the arc surface body 743 rotates between the cylinder 741 and the hemispherical shell 742 with the first gear 744 as the center, the upper protrusion 7431 slides in the first arc groove 7411, and the lower protrusion 7432 slides in the second arc groove 7421, and the lower protrusion 7432 drives the stirring rod 75 to adjust the inclination angle, and continuously adjusts the inclination angle of the stirring rod 75 to continuously change the stirring amplitude of the stirring rod 75, to ensure that the grain powder and the ethanol solution are completely stirred and mixed evenly, to avoid sedimentation causing sample sampling errors.

[0049] The working principle of the present invention is as follows: the unloading and loading conveyor belt 3 transports the grain sample cup into the cabinet 2, and ethanol solution is added to the sample cup. The output end of the second cylinder 73 pushes the frame 745 to move, and the tooth surface between the internal gear pair 7451 and the first gear 744 is engaged. The arc surface body 743 rotates between the cylinder 741 and the hemispherical shell 742 with the first gear 744 as the center of the circle. The upper protrusion 7431 slides in the first arc groove 7411, and the lower protrusion 7432 slides in the second arc groove 7421, driving the stirring rod 75 to adjust the tilt angle, driving the electric The machine 72 outputs a fixed axis torque to drive the second cylinder 73 to rotate on the fixed axis, and continuously adjusts the inclination angle of the stirring rod 75, so that the stirring amplitude of the stirring rod 75 is constantly changed to ensure that the grain powder and the ethanol solution are completely stirred and mixed evenly, avoiding the sediment from causing sample sampling errors. The transport module 4 transports the sample cup to the detection station, and the pipetting mechanism 5 is cleaned with clean water in the cleaning tank 52. The output end of the servo cylinder 553 initially moves away from the rotary spring seat 551, and the pipette gun 57 is downward. The output end of the servo cylinder 553 moves upward, and the slide 5 The round frame 5541 on 54 pulls the rotating column 555, and the rotating column 555 moves in the wave groove 5521 toward one end close to the rotary spring seat 551 and rotates synchronously. The rotating column 555 is located in the wave groove 5521 near one end of the rotary spring seat 551, and the tip of the pipette 57 is upward and located between the two sets of cleaning columns 566. In the initial state, the slide 568 is located near one end of the first cylinder 567. At this time, the spring slider 563 is located in the side slide groove 5621 away from the end of the protruding column 5622. Then the output end of the first cylinder 567 pushes the slide 568 away The two groups of column buckles 564 move toward each other along the inclined slide groove 5681, and the two groups of cleaning columns 566 move toward each other and stick to the tip of the pipette 57. The servo motor 565 outputs a fixed-axis torque to the cleaning column 566. The two groups of cleaning columns 566 rotate synchronously in the opposite direction of the torque to self-clean the surface of the tip of the pipette 57. After self-cleaning, the pipette 57 is turned over to extract the sample solution in the sample cup and drop it onto the detection platform 6. The fungal toxins and heavy metals in the grain sample solution are detected by time-resolved fluorescence immunochromatography and colloidal gold immunochromatography respectively.

[0050] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the invention can be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the invention is defined by the appended claims, not the foregoing description, and all variations within the meaning and range of equivalents of the claims are intended to be included therein. Any reference sign in a claim should not be construed as limiting the claim to which it relates.

Claims

1. Two-in-one fully automatic rapid detector for mycotoxins and heavy metals, featuring: The detector comprises a base (1), a cabinet (2), a loading and unloading conveyor belt (3), a transport module (4), a pipetting mechanism (5), a detection platform (6), a stirring mechanism (7) and a control panel (8), wherein the pipetting mechanism (5) comprises a three-axis displacement module (51) and a cleaning tank (52), the detection platform (6) comprises a base frame (61), the stirring mechanism (7) comprises a two-axis displacement module (71) and a second cylinder (73), the cabinet (2), the loading and unloading conveyor belt (3), the three-axis displacement module (51), the cleaning tank (52), the base frame (61) and the two-axis displacement module (71) are all fixedly connected to the base (1), the control panel (8) is fixedly connected to the cabinet (2), and the control panel (8) is connected to the three-axis displacement module (51) and the second cylinder (73) via electrical signals; The pipetting mechanism (5) further includes a sampling mechanism (53), wherein the sampling mechanism (53) includes a side plate (54), and the side plate (54) is fixedly connected to the three-axis displacement module (51); The sampling mechanism (53) further includes a turnover mechanism (55), a self-cleaning mechanism (56) and a liquid transfer gun (57); the turnover mechanism (55) includes a rotary spring seat (551), a servo cylinder (553) and a rotating column (555); the self-cleaning mechanism (56) includes a side frame (561); the rotary spring seat (551), the servo cylinder (553) and the side frame (561) are all fixedly connected to the side plate (54); and the rotating column (555) is fixedly connected to the liquid transfer gun (57); The turning mechanism (55) further comprises a knife-shaped plate (552) and a slide (554), wherein the knife-shaped plate (552) is rotatably connected to the rotary spring seat (551), the slide (554) is fixedly connected to the output end of the servo cylinder (553), the slide (554) contacts the knife-shaped plate (552), a wave groove (5521) is provided on the knife-shaped plate (552), and a circular frame (5541) is provided on the slide (554), the rotating column (555) is rotatably connected to the wave groove (5521), and the rotating column (555) contacts the circular frame (5541).

2. The two-in-one fully automatic rapid detector for mycotoxins and heavy metals according to claim 1, characterized in that: The self-cleaning mechanism (56) further comprises a box (562), a spring slider (563), a column buckle (564), a servo motor (565), a cleaning column (566), a first cylinder (567) and a slide plate (568). The box (562) is provided with a side slide groove (5621) and a convex column (5622). The side slide groove (5621), the spring slider (563), the column buckle (564) and the cleaning column (566) are each provided with two groups. The spring slider (563) is slidably connected to the side slide groove (5621). The column buckle (564) is fixedly connected to the spring slider (563) and the servo motor (565). The cleaning column (566) is provided with a toothed disc (5661). The spring slider (563) is fixedly connected to the toothed disc (5661). ) is rotatably connected, the spring slider (563) is provided with a first through hole (5631), the column buckle (564) is provided with a second through hole (5641), the output end of the servo motor (565) is in contact with both the first through hole (5631) and the second through hole (5641), the output end of the servo motor (565) is rotatably connected to the cleaning column (566), the first cylinder (567) is fixedly connected to the box (562), the output end of the first cylinder (567) is fixedly connected to the slide plate (568), the slide plate (568) is provided with an inclined slide groove (5681) and a straight groove (5682), the column buckle (564) is slidably connected to the inclined slide groove (5681), and the convex column (5622) is slidably connected to the straight groove (5682).

3. The two-in-one fully automatic rapid detector for mycotoxins and heavy metals according to claim 1, characterized in that: The detection platform (6) further comprises a fluorescence detection platform (62) and a colloidal gold detection platform (63), and the fluorescence detection platform (62) and the colloidal gold detection platform (63) are both fixedly connected to the base frame (61).

4. The two-in-one fully automatic rapid detector for mycotoxins and heavy metals according to claim 1, characterized in that: The stirring mechanism (7) further comprises a driving motor (72), an angle adjustment mechanism (74) and a stirring rod (75); the angle adjustment mechanism (74) comprises a barrel (741), an arcuate body (743) and a square frame (745); the driving motor (72) is fixedly connected to the two-axis displacement module (71); the second cylinder (73) is fixedly connected to the output end of the driving motor (72) and the barrel (741); the output end of the second cylinder (73) is fixedly connected to the square frame (745); a lower protrusion (7432) is provided on the arcuate body (743); and the lower protrusion (7432) is fixedly connected to the stirring rod (75).

5. The two-in-one fully automatic rapid detector for mycotoxins and heavy metals according to claim 4, characterized in that: The angle adjustment mechanism (74) further includes a hemispherical shell (742) and a first gear (744); the hemispherical shell (742) is fixedly connected to the cylinder (741); an upper protrusion (7431) is provided on the arc surface body (743); a first arc groove (7411) is provided on the cylinder (741); a second arc groove (7421) is provided on the hemispherical shell (742); the upper protrusion (7431) is slidably connected to the first arc groove (7411); the lower protrusion (7432) is slidably connected to the second arc groove (7421); the first gear (744) is fixedly connected to the upper protrusion (7431); an internal gear pair (7451) is provided on the square frame (745); and the first gear (744) meshes with the tooth surfaces of the internal gear pair (7451).

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