Ceramide and seaweed repairing composition as well as preparation method and application thereof

Abstract

The invention discloses a ceramide and seaweed repairing composition. The ceramide and seaweed repairing composition is prepared from kappaphycus alvarezii extract, algae oligosaccharide and ceramide, the composition is prepared from the following components in parts by weight: 0.4 to 0.6 part of kappaphycus alvarezii extract, 0.1 to 0.2 part of algae oligosaccharide and 3 to 5 parts of ceramide. The invention also discloses a preparation method of the liposome of the composition, which comprises the step of mixing the kappaphycus alvarezii extract and the algae oligosaccharide aqueous solution with a ceramide organic solution to obtain a liposome suspension, and performing ultrasonic treatment and ultrahigh-pressure microjet treatment on the liposome suspension to obtain the liposome. The kappaphycus alvarezii extract, the algae oligosaccharide and the ceramide in the composition have obvious synergistic effects in the aspects of skin repairing, soothing, moisturizing and the like, and can be widely added into cosmetics or skin care products for use.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and specifically relates to a repair composition of ceramide and seaweed, a preparation method thereof, and an application thereof. Background Art

[0002] Nowadays, people's attention to the skin has increased sharply, and they also attach great importance to skin management. Most people have skin problems. Due to reasons such as seasonal changes, stress, irregular life, and external damage, the skin is prone to sensitivity and damage. Data shows that one in every three women has sensitive skin; the skin barrier is composed of a sebum film and a stratum corneum. After being damaged, various physiological activities of the skin are hindered, and it is prone to dryness, flushing, and itching, leading to various skin problems; although some current repair products will not cause further skin sensitivity and damage, their repair effect is weak, only having a general moisturizing effect, and the effect is not obvious. They cannot immediately repair and soothe the damaged skin. Some have a certain repair effect, but the active ingredients are difficult to reach the dermis for deep repair. The inflammation at the bottom layer of the skin still exists, and it cannot provide a protective and repair effect. The damaged skin is still in a state of being easily infected, prone to further damage and deterioration. In addition, some irritating ingredients staying on the face for too long will cause further damage to the skin.

[0003] The complete skin barrier includes multiple aspects, including five major aspects: microbial barrier, physical barrier, chemical barrier, immune barrier, and pigment barrier. A healthy skin barrier, like a city wall, has a complete structure, including a sebum film and a stratum corneum. A complete sebum film coverage and a sound stratum corneum are the best guardians of the skin. However, behaviors such as excessive cleaning, lack of attention to moisturization, frequent use of periodic skin care products, and inadequate sun protection will all damage the skin barrier. Once the skin barrier is damaged, the skin will become fragile and sensitive, and at the same time, the moisturizing function will decline, and it is prone to symptoms such as dry peeling, flushing, burning, tightness, and itching. At the same time, after the barrier is damaged, due to the problem of water shortage and dryness, it is also easy to cause pigmentation and fine lines, looking "10 years older".

[0004] In recent years, functional natural active extracts have become a research hotspot at home and abroad. Their action mechanisms have the characteristics of multiple targets and multiple levels, and can take effect at multiple action nodes in the mechanism of mediating allergic reactions. In addition, natural active extracts are safe and mild, and have a wide market acceptance. However, there are still few related products with skin repair functions at present, and the effects are average. Therefore, how to develop a safe, effective, and product with obvious skin repair function has become the key concern of technical personnel in the current cosmetics industry. Summary of the Invention

[0005] To overcome the problems of the prior art, the inventors explored different methods and conducted extensive screening of different cosmetic ingredients. Unexpectedly, a skin repair composition was discovered during the research. The components in the composition have a synergistic effect, producing unexpected technical effects. Based on this discovery, the present invention provides the following technical solutions.

[0006] In a first aspect, the present invention provides a skin repair composition comprising Kappaphycus alvarezii extract, oligosaccharides, and ceramides.

[0007] In one or more embodiments, by weight, the composition comprises 0.4 - 0.6 parts of Kappaphycus alvarezii extract, 0.1 - 0.2 parts of oligosaccharides, and 3 - 5 parts of ceramides.

[0008] In one or more embodiments, the ceramide is one or more of ceramide NP, ceramide AS, ceramide AP, and ceramide NS / ceramide NG.

[0009] In one or more embodiments, the ceramide is composed of ceramide NP and ceramide AS in a mass ratio of 8:1 - 12:1.

[0010] In a second aspect, the present invention provides a method for preparing liposomes of any of the above - mentioned compositions. The method includes the step of mixing an aqueous solution of Kappaphycus alvarezii extract and oligosaccharides with an organic solution of ceramide to obtain a liposome suspension, and the step of subjecting the liposome suspension to ultrasonic treatment and microfluidization treatment to obtain the liposomes.

[0011] In one or more embodiments, the preparation method includes the following steps:

[0012] (1) Extract dissolution: After accurately weighing the Kappaphycus alvarezii extract and oligosaccharides in proportion, dissolve them in an appropriate amount of deionized water, stir with a magnetic stirrer until completely dissolved, prepare an aqueous solution of a certain concentration, and add ultrasonic assistance for dissolution to ensure the full dispersion of water - soluble components; the concentration of the Kappaphycus alvarezii extract is 10 - 20 mg / mL, and the concentration of the oligosaccharides is 5 - 10 mg / mL; the ultrasonic assistance for dissolution can select appropriate conditions according to the actual situation, such as ultrasonic treatment at 30 kHz for 5 minutes;

[0013] (2) Ceramide pretreatment: Premix the ceramide with hydrogenated lecithin at a molar ratio of 1:5 and dissolve it in a chloroform - methanol solution to form an organic phase; the concentration of the ceramide solution is 5 - 10 mg / mL; the volume ratio of chloroform to methanol in the chloroform - methanol solution is 3:1;

[0014] (3) Removal of organic solvent: Place the mixed ceramide organic phase in a rotary evaporator. Under reduced pressure, slowly heat and rotate the evaporator to gradually volatilize the organic solvent, and finally form a uniform lipid film on the container wall;

[0015] (4) Preparation of liposome suspension: Slowly add the prepared aqueous solution of Kappaphycus alvarezii extract and algooligosaccharides to the container containing the lipid film, and continuously stir during the addition process to hydrate the lipid in the aqueous solution and form a thick liposome suspension;

[0016] (5) Ultrasonic treatment: Use an ultrasonic cell disruptor to perform ultrasonic treatment on the liposome suspension. Set the ultrasonic power to 20W → 25W → 30W, perform intermittent ultrasonic treatment, ultrasonic for 10s each time, cool down at an interval of 10 seconds during the cycle, and the total ultrasonic time is 10 minutes to make the liposome suspension more uniform;

[0017] (6) High-pressure microfluidization treatment: Transfer the ultrasonically treated liposome suspension to a high-pressure microfluidization device, set the pressure to 300 - 400 MPa, and perform microfluidization treatment; cycle 3 - 5 times, cool down at an interval of 10 seconds each time; immediately transfer the liposome solution to an ice bath after treatment to prevent high temperature from damaging the structure;

[0018] (7) Purification: Use dialysis with a molecular weight cut-off of 10 kDa to purify the liposome solution to remove the unencapsulated components and obtain the liposomes of the said composition.

[0019] In one or more embodiments, the average particle size of the liposomes obtained by the said preparation method is ≤100 nm, and the PDI ≤ 0.06.

[0020] In a third aspect, the present invention provides the application of the liposomes obtained from the composition or the preparation method described in any one of the above in cosmetics or skin care products.

[0021] In one or more embodiments, the addition amount of the said composition or liposomes in cosmetics or skin care products is 6% - 9%.

[0022] Fourthly, the present invention provides a skin care essence. Calculated by weight ratio of the essence, the essence comprises the following components: 5-8% of the composition according to any one of the present invention, 0.5%-1% of ergothioneine, 0.5%-8% of ectoin, 0.8%-1.2% of taurine, 0.3%-1% of hydroxyproline, 0.3%-1% of sodium hyaluronate, 0.02%-0.08% of β-glucan, 0.5%-1.5% of inositol, 0.12%-0.18% of camellia extract, 0.2%-0.6% of centella asiatica extract, 0.1%-0.3% of white water lily extract, 0.5%-0.8% of haematococcus pluvialis extract, 0.2%-0.3% of lithospermum erythrorhizon extract, 0.3%-0.5% of crocus sativus extract, 2%-5% of 1,2-hexanediol, 3%-4% of 1,2-pentanediol, 0.5%-1.5% of allantoin, 0.1%-0.5% of p-hydroxyacetophenone, 1%-2% of polyglyceryl-10 oleate, 0.5%-0.8% of sodium benzoate, 2%-8% of glycerol, 1%-6% of lecithin, and 0.1%-1% of hydrolyzed sclerotium gum, and the balance is water.

[0023] Compared with the prior art, the present invention has the following advantages:

[0024] By compounding the extracts of Kappaphycus alvarezii, oligosaccharides and ceramides in a specific ratio and cooperating synergistically with each other, the present invention can not only quickly repair skin damage, but also has obvious soothing and moisturizing effects, enabling consumers to present a healthy skin appearance. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a graph showing the change in the average value of skin TEWL in Test Example 2.

[0026] Figure 2 It is a graph comparing the effects of the liposomes in Test Example 3. DETAILED DESCRIPTION OF THE INVENTION

[0027] Kappaphycus alvarezii is a common tropical red alga, and its extract is widely used in skin care products, mainly due to its moisturizing, antioxidant and soothing effects. In existing reports, it can be combined with niacinamide, vitamin C, etc. to enhance the brightening effect.

[0028] Oligosaccharides are a class of oligosaccharides extracted from marine algae, which have unique biological activities and wide application values. Specifically, they can be used to develop anti-cardiovascular drugs (such as propylene glycol alginate sulfate sodium salt), antiviral drugs (such as polyglycoglyceride), and immunomodulators; added to dairy products and beverages, they have both nutritional enhancement and intestinal regulation and detoxification functions, and are suitable for special populations (such as diabetic patients); used as a moisturizing cream and whitening essence (utilizing its antioxidant and melanin-inhibiting effects) in cosmetics.

[0029] Ceramide is a lipid widely present in the cell membranes of the human body, especially abundant in the stratum corneum of the skin, and plays a key role in maintaining the skin barrier function, moisturizing, and locking in water.

[0030] In experimental research, the inventors accidentally discovered a skin repair composition, which is a combination of extracts of Kappaphycus alvarezii, oligosaccharides, and ceramide in a specific ratio. This composition has a synergistic effect and has an obvious skin repair effect.

[0031] The composition of the present invention can be prepared into liposomes for better absorption and utilization by the skin.

[0032] It should be understood that the composition or liposome of the present invention has an obvious skin repair effect, so it can be widely added to cosmetics or skin care products for use.

[0033] As is well known to those skilled in the art, the cosmetics or skin care products may include the composition of the present invention and excipients acceptable in the art.

[0034] In one or more embodiments, the acceptable excipients are selected from surfactants, vitamins, natural extracts, preservatives, antioxidants, solubilizers, chelating agents, proteins, amino acids, humectants, fragrances, emollients, penetrants, thickeners, viscosity regulators, film formers, emulsifiers, opacifiers, propellants, carriers, salts, buffers, antistatic agents, antioxidants, and combinations thereof.

[0035] In the present invention, acceptable excipients can be any known such carriers used alone or in combination with other carriers. Useful carriers include water, emollients, fatty acids, fatty alcohols, thickeners, and combinations thereof. Carriers can be aqueous, anhydrous, or emulsions. Preferably, the composition is aqueous, especially water and oil emulsions of the W / O or O / W or triple W / O / W type. When present, the amount of water can be 5-95% by weight of the composition, preferably 20-70%, optimally 35-60%. Emollient materials can be used as carriers acceptable in cosmetics. These can be in the form of silicone oils, natural or synthetic esters, and hydrocarbons.

[0036] In the present invention, thickeners can be used as part of the acceptable excipients. Typical thickeners include cross-linked acrylates (such as Carbopol ), hydrophobically modified acrylates (such as Carbopol ), polyacrylamides (such as Sepigel ), acryloyl methyl propane sulfonic acid / salt polymers and copolymers (such as Aristoflex and )), cellulose derivatives, and natural gums. Suitable cellulose derivatives include sodium carboxymethyl cellulose, hydroxypropyl methyl cellulose, hydroxypropyl cellulose, hydroxyethyl cellulose, ethyl cellulose, and hydroxymethyl cellulose. Natural gums suitable for use in the present invention include guar gum, xanthan gum, sclerotium, carrageenan, pectin, and combinations of these gums. Inorganic substances can also be used as thickeners, especially clays such as bentonite and hectorite, fumed silica, talc, calcium carbonate, and silicates such as magnesium aluminum silicate.

[0037] In the present invention, humectants can be used as part of the acceptable excipients. These are generally polyol materials. Typical polyols include glycerol, propylene glycol, dipropylene glycol, polypropylene glycol, polyethylene glycol, sorbitol, hydroxypropyl sorbitol, hexylene glycol, 1,3 - butanediol, isopentylene glycol, 1,2,6 - hexanetriol, ethoxylated glycerol, propoxylated glycerol, and mixtures thereof.

[0038] In the present invention, surfactants can be used as part of the acceptable excipients. When present, the total concentration of the surfactant can be 0.1 - 90% by weight of the composition, preferably 1 - 40%, optimally 1 - 20%, and highly depends on the type of the personal care product. Surfactants can be selected from anionic, non - ionic, cationic, and amphoteric active substances. Particularly preferred non - ionic surfactants are those in which C10 - C20 fatty alcohols or acidic hydrophobes are condensed with 2 - 100 moles of ethylene oxide or propylene oxide per mole of hydrophobe; C2 - C10 alkylphenols condensed with 2 to 20 moles of alkylene oxide; mono - and di - fatty acid esters of ethylene glycol; fatty acid monoglycerides; sorbitan, mono - and di - C8 - C20 fatty acids; and polyoxyethylene sorbitan and combinations thereof. Alkyl polyglycosides and sugar - based fatty acid amides (such as methyl glucamide) and trialkylamine oxides are also suitable non - ionic surfactants. Preferred anionic surfactants include soaps, alkyl ether sulfates and sulfonates, alkyl sulfates and sulfonates, alkyl benzene sulfonates, alkyl and dialkyl sulfosuccinates, C8 - C20 acyl - hydroxyethyl sulfonates, C6 - C20 alkyl ether phosphates, C8 - C20 sarcosinates, C8 - C20 acyl lactates, sulfacetates, and combinations thereof. Useful amphoteric surfactants include cocamidopropyl betaine, C12 - C20 trialkyl betaine, sodium lauroamphoacetate, and sodium lauroamphodiacetate.

[0039] In the present invention, sunscreen agents can be used as part of the acceptable excipients. Particularly preferred are substances such as ethylhexyl methoxycinnamate, avobenzone, and benzophenone - 3 (also known as oxybenzone). Inorganic sunscreen active substances, such as fine titanium dioxide and zinc oxide, can be used. When present, the amount of the sunscreen agent can generally be 0.1 - 30% by weight of the composition or formulation, preferably 2 - 20%, optimally 4 - 10%.

[0040] Examples

[0041] The following specific examples are used to illustrate the embodiments of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention.

[0042] Before further describing the specific embodiments of the present invention, it should be understood that the protection scope of the present invention is not limited to the specific embodiments described below; it should also be understood that the terms used in the embodiments of the present invention are for describing specific embodiments, rather than limiting the protection scope of the present invention.

[0043] When the embodiments give a numerical range, it should be understood that unless otherwise specified in the present invention, any value between the two endpoints of each numerical range and the two endpoints can be selected. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which the present invention belongs.

[0044] In the embodiments of the present invention, the extracts of Kappaphycus alvarezii, Macrocystis pyrifera, and algooligosaccharides (brown algooligosaccharides) are from Qingdao Ocean University Marine Oligosaccharide Technology Co., Ltd.; ceramides are from Chongqing Zhihe Biopharmaceutical Co., Ltd.

[0045] For those not specified with specific techniques or conditions in the embodiments, they shall be carried out according to the techniques or conditions described in the literature in this field or according to the product specifications. For reagents or instruments not specified with the manufacturer, they are all conventional products that can be obtained through regular channels.

[0046] In the embodiments, the addition amounts, contents, and concentrations of various substances are involved. Among them, unless otherwise specified, the percentage content refers to the mass percentage content.

[0047] Example 1: A skin repair composition

[0048] A skin repair composition, by weight, the composition comprises 0.5 parts of extract of Kappaphycus alvarezii, 0.1 part of algooligosaccharides, and 4 parts of ceramides, and the ceramides are ceramide NP. Mix each component in proportion.

[0049] Example 2: A skin repair composition

[0050] A skin repair composition, by weight, the composition comprises 0.6 parts of extract of Kappaphycus alvarezii, 0.2 part of algooligosaccharides, and 5 parts of ceramides, and the ceramides are ceramide AS. Mix each component in proportion.

[0051] Example 3: A skin repair composition

[0052] A skin repair composition, by weight, the composition comprises 0.4 parts of Kappaphycus alvarezii extract, 0.2 parts of algal oligosaccharides, and 3 parts of ceramide, wherein the ceramide is ceramide AP. Mix the components in proportion.

[0053] Example 4: A skin repair composition

[0054] A skin repair composition, by weight, the composition comprises 0.4 parts of Kappaphycus alvarezii extract, 0.1 parts of algal oligosaccharides, and 5 parts of ceramide, wherein the ceramide is ceramide NS / ceramide NG. Mix the components in proportion.

[0055] Example 5: A skin repair composition

[0056] A skin repair composition, by weight, the composition comprises 0.6 parts of Kappaphycus alvarezii extract, 0.1 parts of algal oligosaccharides, and 5 parts of ceramide, wherein the ceramide is composed of ceramide NP and ceramide AS in a mass ratio of 5:1. Mix the components in proportion.

[0057] Example 6: A skin repair composition

[0058] A skin repair composition, by weight, the composition comprises 0.6 parts of Kappaphycus alvarezii extract, 0.1 parts of algal oligosaccharides, and 5 parts of ceramide, wherein the ceramide is composed of ceramide NP and ceramide AS in a mass ratio of 8:1. Mix the components in proportion.

[0059] Example 7: A skin repair composition

[0060] A skin repair composition, by weight, the composition comprises 0.6 parts of Kappaphycus alvarezii extract, 0.1 parts of algal oligosaccharides, and 5 parts of ceramide, wherein the ceramide is composed of ceramide NP and ceramide AS in a mass ratio of 10:1. Mix the components in proportion.

[0061] Example 8: A skin repair composition

[0062] A skin repair composition, by weight, the composition comprises 0.6 parts of Kappaphycus alvarezii extract, 0.1 parts of algal oligosaccharides, and 5 parts of ceramide, wherein the ceramide is composed of ceramide NP and ceramide AS in a mass ratio of 12:1. Mix the components in proportion.

[0063] Example 9: A skin repair composition

[0064] A skin repair composition. By weight, the composition includes 0.6 parts of Kappaphycus alvarezii extract, 0.1 part of algal oligosaccharides, and 5 parts of ceramide, wherein the ceramide is composed of ceramide NP and ceramide AS in a mass ratio of 15:1. Mix the components in proportion.

[0065] Example 10: A skin repair composition

[0066] A skin repair composition. By weight, the composition includes 0.6 parts of Kappaphycus alvarezii extract, 0.1 part of algal oligosaccharides, and 5 parts of ceramide, wherein the ceramide is composed of ceramide NP and ceramide AP in a mass ratio of 10:1. Mix the components in proportion.

[0067] Example 11: A skin repair composition

[0068] A skin repair composition. By weight, the composition includes 0.6 parts of Kappaphycus alvarezii extract, 0.1 part of algal oligosaccharides, and 5 parts of ceramide, wherein the ceramide is composed of ceramide AS and ceramide AP in a mass ratio of 1:1. Mix the components in proportion.

[0069] Example 12: A skin repair composition

[0070] A skin repair composition. By weight, the composition includes 0.6 parts of Kappaphycus alvarezii extract, 0.1 part of algal oligosaccharides, and 5 parts of ceramide, wherein the ceramide is composed of ceramide NP, ceramide AS, and ceramide AP in a mass ratio of 10:1:1. Mix the components in proportion.

[0071] Example 13: A skin repair composition

[0072] A skin repair composition. By weight, the composition includes 0.6 parts of Kappaphycus alvarezii extract, 0.1 part of algal oligosaccharides, and 5 parts of ceramide, wherein the ceramide is composed of ceramide AS, ceramide AP, and ceramide NS / ceramide NG in a mass ratio of 1:1:1. Mix the components in proportion.

[0073] Example 14: A skin repair composition

[0074] A skin repair composition. By weight, the composition includes 0.6 parts of Kappaphycus alvarezii extract, 0.1 part of algal oligosaccharides, and 5 parts of ceramide, wherein the ceramide is composed of ceramide NP, ceramide AS, ceramide AP, and ceramide NS / ceramide NG in a mass ratio of 10:1:1:1. Mix the components in proportion.

[0075] Comparative Example 1: A skin repair composition

[0076] A skin repair composition, by weight, the composition comprises 0.6 parts of kelp extract, 0.1 part of algal oligosaccharides and 5 parts of ceramide, wherein the ceramide is composed of ceramide NP and ceramide AS in a mass ratio of 10:1. Mix each component in proportion.

[0077] Comparative Example 2: A skin repair composition

[0078] A skin repair composition, by weight, the composition comprises 0.3 parts of kelp extract, 0.3 parts of Kappaphycus alvarezii extract, 0.1 part of algal oligosaccharides and 5 parts of ceramide, wherein the ceramide is composed of ceramide NP and ceramide AS in a mass ratio of 10:1. Mix each component in proportion.

[0079] Comparative Example 3: A skin repair composition

[0080] A skin repair composition, by weight, the composition comprises 0.1 part of algal oligosaccharides and 5 parts of ceramide, wherein the ceramide is composed of ceramide NP and ceramide AS in a mass ratio of 10:1. Mix each component in proportion.

[0081] Comparative Example 4: A skin repair composition

[0082] A skin repair composition, by weight, the composition comprises 0.6 parts of Kappaphycus alvarezii extract and 0.1 part of algal oligosaccharides. Mix each component in proportion.

[0083] Comparative Example 5: A skin repair composition

[0084] A skin repair composition, by weight, the composition comprises 0.6 parts of Kappaphycus alvarezii extract and 5 parts of ceramide, wherein the ceramide is composed of ceramide NP and ceramide AS in a mass ratio of 10:1. Mix each component in proportion.

[0085] Detection Example 1: Experiment on promoting the scratch healing of skin keratinocytes

[0086] Experimental principle: The cell scratch experiment is a simple and rapid method for measuring cell migration and repair ability, similar to an in vitro wound healing model. Detecting the cell migration rate can reflect the barrier repair ability of cells. A large migration rate indicates good barrier repair ability of the test sample; a small migration rate indicates poor barrier repair ability of the test sample.

[0087] Samples to be tested: The compositions of Examples 1-14 and Comparative Examples 1-5 were diluted with physiological saline to a certain concentration (concentration gradients were 1.5 v / v%, 1 v / v%, 0.5 v / v%). Blank control: physiological saline. Each sample to be tested was set at concentrations of 1.5 v / v%, 1 v / v%, and 0.5 v / v%, and three parallel controls were set for each concentration, and the results of the three parallel controls were averaged.

[0088] Experimental protocol: HaCat cells were seeded at 4×10 4 / well in cell inserts and cultured in a cell incubator. When the cell density reached approximately 80%, the liquid in the cell inserts and the 6-well plates was removed, and 2 ml of each sample to be tested at different concentrations (concentration gradients were 1.5, 1, 0.5 v / v%) was added, and they were co-cultured for 24 h. Photos were taken at 0 h and 24 h, and the cell area was calculated using ImageJ, and the corresponding migration rate was calculated.

[0089] Result calculation formula:

[0090] Migration rate = (area at 0 h - area at 24 h) / area at 0 h

[0091] Relative migration rate = migration rate of sample - migration rate of blank group.

[0092] The results are shown in Table 1:

[0093] Table 1 Effects of the compositions described in Examples and Comparative Examples on the migration rate of skin keratinocytes

[0094]

[0095]

[0096] From the above results, it can be seen that the compositions described in Examples 1-14 have a significantly higher migration rate of skin keratinocytes than those in Comparative Examples 1-5, and as the concentration of the composition increases, the migration rate of skin keratinocytes also increases. Among them, when ceramide is a combination of ceramide NP and ceramide AS, compared with adding ceramide NP, AS, AP, NS / NG alone, or compared with the combination of other two, three, or four ceramides, the corresponding composition has a significantly higher migration rate of skin keratinocytes. Further, when ceramide is a combination of ceramide NP and ceramide AS at a mass ratio of 8:1 - 12:1, the effect on the migration rate of skin keratinocytes is the most significant, and the relative migration rate of the corresponding composition to skin keratinocytes at a concentration of 1.5 v / v% is greater than 30%.

[0097] The inventors noticed that in the prior art, extracts of Macrocystis pyrifera and Kappaphycus alvarezii are generally used in combination or alternatively. Therefore, Comparative Example 1 and Comparative Example 2 were set up in the present invention, where the extract of Macrocystis pyrifera completely replaced the extract of Kappaphycus alvarezii and the extract of Macrocystis pyrifera replaced part of the extract of Kappaphycus alvarezii, respectively. The experimental results showed that when the extract of Macrocystis pyrifera was combined with oligosaccharides and ceramides in the specific ratio described in the present invention, its effect was significantly inferior to that of the composition of Kappaphycus alvarezii. In addition, in Comparative Examples 3-5, the extract of Kappaphycus alvarezii, ceramides, and oligosaccharides in the composition were deleted respectively, and the experimental data showed that the migration rate of skin keratinocytes was significantly reduced.

[0098] In summary, when the components of the skin repair composition described in the present invention are compounded in a specific ratio, they have a synergistic effect and obvious skin repair efficacy.

[0099] Detection Example 2: Human test experiment

[0100] 1. Volunteer recruitment:

[0101] Volunteer inclusion criteria: (1) Asian women aged 18-60 who are not pregnant or lactating; (2) no acute inflammation or other serious skin diseases on the face; (3) not highly sensitive and not allergic to cosmetics; (4) not participated in similar clinical trials in the past month; (5) positive for lactic acid stinging (lactic acid stinging score ≥ 3 in nasolabial groove and any one side of the cheek). Finally, 33 people were included, aged 23-45, with an average age of 36.53 ± 6.38 years, all meeting the volunteer inclusion criteria.

[0102] 2. Preparation before the test:

[0103] No products can be used on the test site on the day. Before the test, the subjects need to agree to clean their faces, dry them with a lint-free tissue, and sit quietly in the human efficacy evaluation room (temperature 20-22°C, humidity: 40-60%) for 30 minutes. During this period, they cannot drink water or beverages. The subjects should stay relaxed, with their faces exposed, and avoid touching.

[0104] Before the test, the subjects were given an explanation of the test and signed an informed consent form. The test area was the face.

[0105] 3. Test content

[0106] 3.1. Analysis of skin soothing effect

[0107] 3.1.1. Product usage method

[0108] Test samples: the compositions described in Examples 1-2, 5-9 and Comparative Examples 1, 3, 4, diluted to a concentration of 5% with sterile water; the control was sterile water.

[0109] After cleansing the face, take an appropriate amount of the sample (about 1 g) and evenly and quickly apply it to the face until completely absorbed. Thirty-three volunteers were randomly divided into 11 groups, with 3 people in each group. The left and right faces of each person were tested simultaneously. Each group tested one sample, which was equivalent to 6 parts being tested in each group. Six data were obtained for each group, and the average of the 6 data for each group was the average value of the skin redness a* of the corresponding sample.

[0110] 3.1.2, Test method:

[0111] In this test, through a skin facial image analyzer Visia 7 collected red images, and Image-Pro Plus (IPP) analysis software was used to analyze the skin redness a* value to evaluate the soothing effect of the composition described in the present invention.

[0112] The test cycle of this test was 30 s, and the number of skin image acquisitions was 2 times, namely 0 s and 30 s. The greater the difference between the average values of the skin redness a* before and after applying the sample, the better the soothing effect of the corresponding sample.

[0113] 3.1.3, Test results:

[0114] Table 2 Skin redness a* values after using different samples

[0115]

[0116]

[0117] The test results are shown in Table 2, which shows that: the differences in the average values of the skin redness a* after applying the compositions described in Examples 1-2, 5-9 for 30 s all decreased significantly, especially the differences in the average values of the skin redness a* after applying the compositions described in Examples 5-9 were the most significant; although the average values of the skin redness a* decreased to a certain extent after applying the compositions described in Comparative Examples 1, 3, and 4, there was still a large gap compared with other examples.

[0118] Therefore, when the components of the skin repair composition described in the present invention are compounded in a specific ratio, they have a synergistic effect and obvious skin repair and soothing effects.

[0119] 3.2 Analysis of the transepidermal water loss (TEWL) value of the skin

[0120] 3.2.1, Product usage method:

[0121] Test sample: The composition described in Example 7 was diluted to a concentration of 5% with sterile water.

[0122] After cleansing the face, take an appropriate amount of the sample (about 1 g) and evenly apply it to the face until completely absorbed, once in the morning and once in the evening every day for 7 consecutive days. During the test period, the subjects were prohibited from using other repair reagents. The subjects mainly engaged in indoor activities and avoided long-term exposure to outdoor light.

[0123] 3.2.2. Test method:

[0124] Use the skin transdermal water loss probe Tewameter TM Hex to detect the TEWL value of the subjects' skin at the test site. The larger the measured TEWL value, the more the trans-epidermal water loss per unit time and per unit cross-sectional area, and vice versa. Therefore, the decreasing trend of the TEWL value represents the recovery process of trans-epidermal water loss in barrier-damaged skin. By comparing the changes in the average TEWL value of the subjects' skin before and after use, the effect of the sample in reducing skin water loss can be reflected.

[0125] TEWL change rate = (TEWL value after 7 days of using the product (D7) - TEWL value before using the product (D0)) / TEWL value before using the product (D0) × 100%

[0126] 3.2.3. Test results:

[0127] Table 3 Statistics of average skin TEWL values ((g / h·m 2 ), n = 33)

[0128]

[0129]

[0130] Table 4 Analysis of differences in average skin TEWL values ((g / h·m 2 ), n = 33)

[0131] Group Mean ± Standard Deviation P value (vs. D0) D0 22.09±3.84 / D7 18.09±4.09 ＜0.01

[0132] The test results are as Figure 1 shown in Table 3 and Table 4, which show that: compared with the baseline value (D0) before using the composition of the present invention, the average skin TEWL value showed a downward trend after using the product, and decreased by 18.11% after 7 days of use (D7), showing a highly significant difference compared with the baseline value (p < 0.01%), indicating that the composition of the present invention has an obvious skin repair function.

[0133] 3.3. Subjective evaluation of the sample

[0134] Test sample: The composition described in Example 7, diluted to a concentration of 5% with sterile water.

[0135] After 7 days (D7) of using the product, the subjects made subjective evaluations on the soothing effect of the sample, etc., and the statistical results are as follows:

[0136] Table 5 Subjective evaluation results of the soothing effect of the product (n = 33)

[0137]

[0138] From the above subjective evaluations, after 7 days (D7) of using the product of the present invention, the average value of all scores is above 6.2 points, and statistically more than 50% of the subjects gave positive evaluations on the soothing effect and moisturizing effect of the product. Among them, the "soothing skin burning sensation", "relieving stinging, dryness and itching", "improving skin flushing", "enhancing skin tolerance" and "enhancing skin moisturization" all had a 100% approval rate at D7.

[0139] Test Example 3: A liposome and its human test experiment

[0140] In this test example, the composition of Example 7 was prepared into liposomes and then a human trial was conducted.

[0141] A method for preparing a liposome, the method includes the steps of mixing an extract of Kappaphycus alvarezii and an aqueous solution of algooligosaccharides with an organic solution of ceramide to obtain a liposome suspension, and subjecting the liposome suspension to ultrasonic treatment and microfluidization under ultra-high pressure to obtain the liposome, specifically including the following steps:

[0142] (1) Extract dissolution: After accurately weighing the extract of Kappaphycus alvarezii and algooligosaccharides according to the ratio, dissolve them in an appropriate amount of deionized water, stir with a magnetic stirrer until completely dissolved, prepare an aqueous solution with a certain concentration, and add ultrasonic-assisted dissolution (30 kHz, 5 minutes) to ensure that the water-soluble components are fully dispersed. Concentration of Kappaphycus alvarezii extract: 15 mg / mL, concentration of algooligosaccharides: 8 mg / mL.

[0143] (2) Ceramide pretreatment: Pre-mix ceramide (7 mg / mL) and hydrogenated lecithin in a molar ratio of 1:5, and dissolve them in chloroform-methanol (volume ratio 3:1) to form an organic phase;

[0144] (3) Removal of organic solvents: Place the mixed ceramide organic phase in a rotary evaporator, under reduced pressure, slowly heat and rotate to evaporate, so that the organic solvents gradually volatilize, and finally form a uniform lipid film on the container wall;

[0145] (4) Preparation of liposome suspension: Slowly add the prepared aqueous solution of Kappaphycus alvarezii extract and algooligosaccharides to the container containing the lipid film, and continuously stir during the addition process to hydrate the lipid in the aqueous solution to form a coarse liposome suspension;

[0146] (5) Ultrasonic treatment: Use an ultrasonic cell disruptor to perform ultrasonic treatment on the liposome suspension. Set the ultrasonic power to 20W → 25W → 30W, with intermittent ultrasound, 10s of ultrasound each time, and a 10-second cooling interval between cycles. The total ultrasonic time is 10 minutes to fully disperse the water-soluble polymer substances and make the liposome primary emulsion more uniform;

[0147] (6) High-pressure microfluidization treatment: Transfer the liposome suspension after ultrasonic treatment to a high-pressure microfluidization device, set the pressure to 350MPa, and perform microfluidization treatment. Cycle 4 times, with a 10-second cooling interval each time. Immediately transfer the liposome solution to an ice bath after treatment to prevent structural damage caused by high temperature.

[0148] (7) Purification: Use dialysis (cut-off molecular weight 10kDa) to remove the unencapsulated components in the liposome solution, that is, obtain the liposomes of the composition.

[0149] The average particle size and its distribution of the liposomes prepared above were measured using a laser particle size analyzer. The results showed that the average particle size of the liposomes was 78.5nm, and the polydispersity index PDI of the liposomes was 0.05.

[0150] Take an appropriate amount of the liposomes prepared by the above method for application on the human face. Select a volunteer with obvious facial erythema for testing. Take an appropriate amount and apply it to the same part of the face every day. After 3 days of use, the effect was very obvious, as Figure 2 shown.

[0151] Therefore, the components of the present invention have a synergistic skin repair effect. Preparing the composition of the present invention into liposomes can be more fully absorbed and utilized by the human body, and significant effects can be obtained in a shorter time.

[0152] Application example: A serum

[0153] A serum, calculated by total mass percentage, the serum contains the following components: 7% of the composition in Example 7, 0.7% of ergothioneine, 3% of ectoine, 1.1% of taurine, 0.8% of hydroxyprogesterone caproate, 0.5% of sodium hyaluronate, 0.06% of β-glucan, 0.9% of inositol, 0.15% of camellia extract, 0.5% of centella asiatica extract, 0.2% of white water lily extract, 0.6% of haematococcus pluvialis extract, 0.25% of lithospermum erythrorhizon extract, 0.4% of crocus sativus extract, 4% of 1,2 - hexanediol, 3% of 1,2 - pentanediol, 0.9% of allantoin, 0.3% of 4 - hydroxyacetophenone, 1% of polyglyceryl - 10 oleate, 0.8% of sodium benzoate, 5% of glycerol, 4% of lecithin, and 0.3% of hydrolyzed sclerotium gum, and the rest is water.

[0154] The above-described embodiments merely represent several implementation manners of the present invention, which are convenient for understanding the technical solutions of the present invention specifically and in detail, but should not be construed as limiting the protection scope of the invention patent. It should be noted that for those of ordinary skill in the art, several modifications and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention.

Claims

1. A skin repair composition, characterized in that, It contains Kappaphycus alvarezii extract, oligosaccharides and ceramide.

2. The composition according to claim 1, wherein By weight, the composition includes 0.4 - 0.6 parts of Kappaphycus alvarezii extract, 0.1 - 0.2 parts of oligosaccharides and 3 - 5 parts of ceramide.

3. The composition according to claim 1, wherein The ceramide is one or more of ceramide NP, ceramide AS, ceramide AP and ceramide NS / ceramide NG.

4. The composition according to claim 3, characterized in that, The ceramide is composed of ceramide NP and ceramide AS in a mass ratio of 8:1 - 12:

1.

5. A method for preparing liposomes of the composition according to any one of claims 1-4, characterized in that, The method includes the steps of mixing the Kappaphycus alvarezii extract and the aqueous solution of oligosaccharides with the organic solution of ceramide to obtain a liposome suspension, and the step of obtaining the liposome after subjecting the liposome suspension to ultrasonic treatment and ultra-high pressure microfluidization treatment.

6. The preparation method according to claim 5, wherein The preparation method includes the following steps: (1) Extract dissolution: After accurately weighing the Kappaphycus alvarezii extract and oligosaccharides in proportion, dissolve them in an appropriate amount of deionized water, stir with a magnetic stirrer until completely dissolved, prepare an aqueous solution with a certain concentration, and add ultrasonic assistance for dissolution to ensure that the water-soluble components are fully dispersed; the concentration of the Kappaphycus alvarezii extract is 10 - 20 mg / mL, and the concentration of the oligosaccharides is 5 - 10 mg / mL; (2) Ceramide pretreatment: Premix ceramide and hydrogenated lecithin in a molar ratio of 1:5, dissolve them in a chloroform-methanol solution to form an organic phase; the concentration of the ceramide solution is 5 - 10 mg / mL; the volume ratio of chloroform to methanol in the chloroform-methanol solution is 3:1; (3) Removal of organic solvents: Place the mixed ceramide organic phase in a rotary evaporator, under reduced pressure conditions, slowly heat and rotate to evaporate, so that the organic solvents gradually volatilize, and finally form a uniform lipid film on the container wall; (4) Preparation of liposome suspension: Slowly add the prepared aqueous solution of Kappaphycus alvarezii extract and oligosaccharides to the container containing the lipid film, and continuously stir during the addition process to hydrate the lipid in the aqueous solution to form a thick liposome suspension; (5) Ultrasonic treatment: Use an ultrasonic cell disruptor to perform ultrasonic treatment on the liposome suspension, set the ultrasonic power to 20W → 25W → 30W, intermittent ultrasonic treatment, ultrasonic for 10s each time, cool down at intervals of 10 seconds during the cycle, and the total ultrasonic time is 10 minutes to make the liposome suspension more uniform; (6) Ultra-high pressure microfluidization treatment: Transfer the ultrasonically treated liposome suspension to an ultra-high pressure microfluidization device, set the pressure to 300 - 400 MPa, and perform microfluidization treatment; cycle 3 - 5 times, cool down at intervals of 10 seconds each time; immediately transfer the liposome solution to an ice bath after treatment to prevent high temperature from damaging the structure; (7) Purification: Adopt dialysis with a molecular weight cut-off of 10 kDa to purify the liposome solution to remove the unencapsulated components and obtain the liposome of the composition.

7. The preparation method according to claim 5 or 6, characterized in that, The obtained liposome has an average particle size ≤100 nm and PDI ≤0.

06.

8. Use of the liposome obtained from the composition according to any one of claims 1 - 4 or the preparation method according to any one of claims 5 - 7 in cosmetics or skin care products.

9. The application according to claim 8, wherein, The addition amount of the composition or liposome in cosmetics or skin care products is 0.5%-9%.

10. A skin care essence, characterized in that, Calculated by weight ratio of the essence, the essence contains the following components: 5%-8% of the composition according to any one of claims 1-4, 0.5%-1% of ergothioneine, 0.5%-8% of ectoin, 0.8%-1.2% of taurine, 0.3%-1% of hydroxyproline, 0.3%-1% of sodium hyaluronate, 0.02%-0.08% of β-glucan, 0.5%-1.5% of inositol, 0.12%-0.18% of camellia extract, 0.2%-0.6% of centella asiatica extract, 0.1%-0.3% of white water lily extract, 0.5%-0.8% of haematococcus pluvialis extract, 0.2%-0.3% of lithospermum erythrorhizon extract, 0.3%-0.5% of crocus sativus extract, 2%-5% of 1,2-hexanediol, 3%-4% of 1,2-pentanediol, 0.5%-1.5% of allantoin, 0.1%-0.5% of p-hydroxyacetophenone, 1%-2% of polyglyceryl-10 oleate, 0.5%-0.8% of sodium benzoate, 2%-8% of glycerol, 1%-6% of lecithin and 0.1%-1% of hydrolyzed sclerotium gum, and the balance is water.

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