Liquid chromatographic column chromatography separation equipment

Abstract

The invention discloses column chromatography separation equipment for a liquid chromatographic column, and particularly relates to the field of liquid chromatographic columns, the column chromatography separation equipment comprises a support frame, a chromatography separation part is arranged on the support frame, the chromatography separation part comprises a column tube, an end cover II is arranged at the bottom end of the column tube, a filter sieve plate is arranged at the top of the end cover II, and a stationary phase is poured into the column tube through the top end of the column tube. The filtering sieve plate is used for filtering stationary phases. The rotating disc is arranged in the piston, the cavity and the outside are in a closed state, the bottom of the piston can apply stable pressure to the stationary phase to ensure that the stationary phase can be uniformly and tightly filled in the column pipe, the through hole II is communicated with the through hole I, and mobile phase liquid is discharged onto the stationary phase through the through holes I which are uniformly formed at equal intervals; the plurality of dispersed through holes I can distribute the mobile phase more uniformly, reduce the impact on the stationary phase, enable the mobile phase to fully participate in the separation process, well separate different components, and improve the separation efficiency.

Description

Technical Field

[0001] The present invention relates to the technical field of liquid chromatography columns. More specifically, the present invention relates to a liquid chromatography column chromatography separation device. Background Art

[0002] A liquid chromatography column is a chromatography column filled with a stationary phase. When a mixture sample enters the liquid chromatography column with the mobile phase, due to the differences in the partition coefficients, adsorption capacities, etc. of the various components in the sample between the stationary phase and the mobile phase, their migration speeds in the chromatography column are different, and it can separate different components in the sample in the column. Liquid chromatography columns are widely used in fields such as pharmaceutical analysis, the food industry, and environmental monitoring. In order to enable the column tube of the liquid chromatography column to accommodate more samples, thereby reducing the number of injections and improving the separation efficiency, a column tube with a larger diameter is selected.

[0003] In the liquid chromatography column in the prior art, a mobile phase delivery tube is arranged inside the piston, and the liquid discharge end of the delivery tube is located at the bottom of the piston. When in use, first, the stationary phase is put into the column tube, and then a linear driving device is used to drive the piston to compact the stationary phase in the column tube, and the piston applies a constant pressure to the stationary phase to ensure that the stationary phase is packed tightly and improve the separation efficiency of the chromatography column. Then, the mobile phase is delivered through the delivery tube. In order to overcome the resistance of the mobile phase to the stationary phase particles, it is necessary to apply pressure to deliver the mobile phase. When the mobile phase passes through the stationary phase, different components are separated in the column tube.

[0004] However, due to the large diameter of the column tube, the position where the mobile phase passes through the stationary phase is relatively single, that is, the mobile phase is unevenly distributed in the stationary phase, and only part of the stationary phase can effectively participate in the separation process. The migration path of the mobile phase in the stationary phase becomes complex, and different components cannot be well separated, resulting in low separation efficiency. Summary of the Invention

[0005] The liquid chromatography column chromatography separation device provided by the present invention aims to solve the following problem: In the existing liquid chromatography column chromatography separation device, due to the large diameter of the column tube, the position where the mobile phase passes through the stationary phase is relatively single, that is, the mobile phase is unevenly distributed in the stationary phase, and only part of the stationary phase can effectively participate in the separation process. The migration path of the mobile phase in the stationary phase becomes complex, and different components cannot be well separated, resulting in low separation efficiency.

[0006] To achieve the above object, the present invention provides the following technical solution: A liquid chromatography column chromatography separation device includes a support frame, on which a chromatography separation component is arranged. The chromatography separation component includes a column tube, and a second end cap is arranged at the bottom end of the column tube. A filter sieve plate is arranged on the top of the second end cap. The stationary phase is poured into the column tube through the top end of the column tube, and the filter sieve plate is used to filter the stationary phase; An extrusion mechanism is provided on the support frame. The extrusion mechanism includes a second linear driver. The output end of the second linear driver is provided with a column pressure maintaining mechanism. The column pressure maintaining mechanism includes a piston. A cavity is formed inside the piston. A through hole one is formed at the bottom of the piston. A rotating disk is rotatably provided on the inner wall of the bottom of the cavity. A through hole two is formed on the rotating disk. A mobile phase delivery pipe is fixedly communicated inside the cavity. The mobile phase delivery pipe is used to deliver the mobile phase into the cavity. The output end of the second linear driver is used to drive the piston to move along the length direction of the column tube inside the column tube. When the through hole one and the through hole two are misaligned, the piston vertically moves downward inside the column tube to apply a constant pressure to the stationary phase. When the through hole one and the through hole two are communicated, the mobile phase inside the cavity enters the stationary phase to achieve separation.

[0007] In a preferred embodiment, a material removing mechanism is provided inside the piston. The material removing mechanism includes a movable shaft. An arc-shaped hole is formed on the rotating disk. The movable shaft is slidably provided in the arc-shaped hole and the through hole one. A guiding groove is formed on the inner wall of the arc-shaped hole. A connecting seat is fixedly provided on the movable shaft. The connecting seat is slidably provided in the guiding groove.

[0008] In a preferred embodiment, a through hole four is formed on the rotating disk. A through hole three is formed on the inner wall of the bottom of the cavity. The through hole three is adapted to the through hole four. The bottom end of the through hole three is communicated with the through hole one. The bottom end of the through hole three is also adapted to the bottom end of the movable shaft.

[0009] In a preferred embodiment, a through hole five is formed on the arc-shaped hole. The through hole five is communicated with the through hole four. The through hole five is used to deliver the mobile phase inside the arc-shaped hole to the through hole four.

[0010] In a preferred embodiment, two support plates are fixedly provided on the support frame. Mounting holes are formed on the support plates. An auxiliary ring is fixedly provided on the support plates. The auxiliary ring is concentric with the mounting hole. Fixing rings are placed inside both auxiliary rings. The diameter of the fixing ring is larger than the diameter of the mounting hole. Two connecting rings are fixedly provided on the column tube. The fixing ring and the corresponding connecting ring are fixed by bolts.

[0011] In a preferred embodiment, the chromatographic separation component further includes a first end cap. The first end cap is fixedly installed at the top end of the column tube. An exhaust hole is formed on the first end cap. The exhaust hole is communicated with the column tube. The output end of the second linear driver is slidably provided with the first end cap. A groove is formed inside the output end of the second linear driver. The mobile phase delivery pipe is located inside the groove. The end of the mobile phase delivery pipe away from the cavity is fixedly communicated with a pump.

[0012] In a preferred embodiment, the extrusion mechanism further includes a first linear driver. The first linear driver is fixedly provided on the support frame. A fixing seat is fixedly provided on the output end of the first linear driver. The second linear driver is fixedly installed on the fixing seat.

[0013] In a preferred embodiment, a rotary driver is fixedly arranged on the piston. The output end of the rotary driver is fixedly arranged at the center point of the rotary disk. A connecting hole is formed in the piston, and a first sealing ring is fixedly arranged on the inner wall of the connecting hole. The output end of the rotary driver is rotatably arranged in the sealing ring.

[0014] In a preferred embodiment, a liquid discharge port is formed in the second end cap. The liquid discharge port is located at the center of the second end cap and is used for discharging the liquid in the column tube.

[0015] In a preferred embodiment, a second sealing ring is fixedly sleeved on the outer side of the piston, and the second sealing ring is in close fit with the inner wall of the column tube.

[0016] The beneficial effects of the present invention are as follows: 1. By arranging a rotary disk in the piston of the present invention, the cavity is in a closed state with the outside. The bottom of the piston can apply a stable pressure to the stationary phase, ensuring that the stationary phase can be filled in the column tube evenly and tightly. By driving the rotary disk to rotate, the second through hole communicates with the first through hole, and the mobile phase liquid is discharged onto the stationary phase through a plurality of first through holes arranged evenly at equal intervals. The dispersed plurality of first through holes can distribute the mobile phase more evenly, reduce the impact on the stationary phase, and enable the mobile phase to fully participate in the separation process, so that different components can be well separated, improving the separation efficiency.

[0017] 2. By arranging a material removing mechanism in the present invention, by driving the rotary disk to rotate, the height of the movable shaft can be increased, preventing packing particles from entering the cavity, and the packing particles will not cause wear to the movable shaft or the rotary disk, thereby ensuring the column efficiency of the chromatographic column and further improving the separation efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 is a three-dimensional structural schematic diagram of the present invention.

[0019] Figure 2 For the present invention Figure 1 is a front view structural schematic diagram.

[0020] Figure 3 is a three-dimensional structural schematic diagram of the support frame of the present invention.

[0021] Figure 4 is a front view sectional structural schematic diagram of the column tube of the present invention.

[0022] Figure 5 is a front view sectional structural schematic diagram of the piston of the present invention.

[0023] Figure 6 For the present invention Figure 5 is a schematic diagram of the moving trajectory of the piston in a bottom view.

[0024] Figure 7Schematic cross-sectional view of the main view of the movable shaft of the present invention.

[0025] Figure 8 For the present invention Figure 7 Schematic diagram of the movement track of the movable shaft in the present invention.

[0026] Figure 9 For the present invention Figure 8 Schematic diagram of the structure of part A in the present invention.

[0027] Figure 10 Schematic three-dimensional structure diagram of the rotating disk of the present invention.

[0028] Figure 11 For the present invention Figure 10 Schematic three-dimensional structure diagram of the movable shaft located in the arc-shaped hole in the present invention.

[0029] Reference numerals are: 1, support frame; 11, support plate; 12, fixing ring; 2, chromatographic separation component; 21, column tube; 211, connecting ring; 22, end cover one; 23, end cover two; 24, filter sieve plate; 3, extrusion mechanism; 31, linear driver one; 32, fixing seat; 33, linear driver two; 4, column pressure maintaining mechanism; 41, piston; 411, cavity; 412, through hole one; 42, rotating disk; 421, through hole two; 43, mobile phase delivery tube; 44, rotating driver; 5, material removal mechanism; 51, movable shaft; 511, connecting seat; 52, arc-shaped hole; 521, guide groove; 522, through hole five; 53, through hole three; 54, through hole four. Detailed implementation manners

[0030] The following further describes the present application in detail with reference to the drawings. It is necessary to point out here that the following specific implementation manners are only used to further illustrate the present application and cannot be construed as limiting the protection scope of the present application. Those skilled in the art can make some non-essential improvements and adjustments to the present application according to the above application content.

[0031] Referring to the attached Figures 1 to 6 , a liquid chromatography column chromatography separation device, including a support frame 1, a chromatographic separation component 2 is arranged on the support frame 1, the chromatographic separation component 2 includes a column tube 21, an end cover two 23 is arranged at the bottom end of the column tube 21, a filter sieve plate 24 is arranged on the top of the end cover two 23, the stationary phase is poured into the column tube 21 through the top end of the column tube 21, and the filter sieve plate 24 is used for filtering the stationary phase; The support frame 1 is provided with an extrusion mechanism 3. The extrusion mechanism 3 includes a linear driver two 33. The output end of the linear driver two 33 is provided with a column pressure maintaining mechanism 4. The column pressure maintaining mechanism 4 includes a piston 41. A cavity 411 is formed inside the piston 41. A through hole one 412 is formed at the bottom of the piston 41. A rotating disk 42 is rotatably arranged on the bottom inner wall of the cavity 411. A through hole two 421 is formed on the rotating disk 42. A mobile phase delivery pipe 43 is fixedly communicated inside the cavity 411. The mobile phase delivery pipe 43 is used to deliver the mobile phase into the cavity 411. The output end of the linear driver two 33 is used to drive the piston 41 to move along the length direction of the column tube 21 inside the column tube 21. When the through hole one 412 and the through hole two 421 are misaligned, the piston 41 vertically moves downward inside the column tube 21 to apply a constant pressure to the stationary phase. When the through hole one 412 and the through hole two 421 are communicated, the mobile phase inside the cavity 411 enters the stationary phase to achieve separation.

[0032] It should be noted that, referring to Figure 6 , a plurality of through holes one 412 are formed at the bottom of the piston 41. The plurality of through holes one 412 are equidistantly arranged at the bottom of the piston 41. The number of the through holes two 421 corresponds to the number of the through holes one 412. An auxiliary hole is formed on the cavity 411. A hole cover is threadedly connected inside the auxiliary hole. When injecting the mobile phase liquid into the cavity 411, the auxiliary hole is used to discharge the gas inside the cavity 411. The hole cover is used to block the auxiliary hole. One end of the mobile phase delivery pipe 43 far away from the cavity 411 is fixedly communicated with a pump. The pump is used to make the mobile phase delivery pipe 43 spray out the mobile phase and apply pressure to the sprayed mobile phase. Even under the action of the resistance of the stationary phase particles, the mobile phase can stably pass through the stationary phase. The stationary phase includes but is not limited to matrix materials such as silica gel and polymer microspheres; chemically bonded phases such as alkyl silane and aryl silane; ion exchange groups such as sulfonic acid groups and quaternary ammonium groups, etc. When packing the stationary phase into the chromatographic column, first dissolve the stationary phase in solvents such as methanol or acetonitrile to make a suspension, fully mix the suspension, and then pour the suspension into the column tube 21. The suspension is the initial state of the stationary phase. When the liquid in the suspension is discharged from the column tube 21, the remaining particulate packing inside the column tube 21 is the stationary phase. The mobile phase includes but is not limited to organic solutions, buffer hydrochloric acid solutions, water and other substances. Based on the difference in the distribution coefficient and the different physical and chemical properties between the stationary phase and the mobile phase, the moving speeds of the mobile phase inside the chromatographic column are different, so as to achieve separation. Finally, the mobile phase passing through the stationary phase generates an effluent. After the effluent is discharged from the column tube 21, use an ultraviolet detector or a mass spectrometer to detect the absorption characteristics of the effluent to ultraviolet light and detect the molecular weight and structure of the effluent substance, so as to achieve qualitative and quantitative analysis of the effluent. The principle of the mobile phase passing through the stationary phase to achieve separation, as a mature existing technology, will not be elaborated too much here.

[0033] It should also be noted that the chromatography separation component 2 further includes a first end cap 22, which is fixedly installed at the top of the column tube 21. An exhaust hole is provided on the first end cap 22, and the exhaust hole is communicated with the column tube 21. The output end of the second linear actuator 33 is slidably arranged with the first end cap 22. A groove is provided in the output end of the second linear actuator 33, and the mobile phase delivery tube 43 is located in the groove. A liquid discharge port is provided on the second end cap 23, and the liquid discharge port is located at the center of the second end cap 23 for discharging the liquid in the column tube 21. The end of the mobile phase delivery tube 43 away from the piston 41 is provided as a flexible tube, which is convenient to adjust the movement of the bottom end of the mobile phase delivery tube 43 according to the movement of the output end of the second linear actuator 33.

[0034] Furthermore, the design of the exhaust hole can prevent negative pressure from being generated in the upper part of the column tube 21 during the movement of the piston 41. A diversion tube is fixedly installed in the liquid discharge port. The first end cap 22 and the second end cap 23 are both fixedly installed on the column tube 21 by bolts, including but not limited to this. A filter sieve plate 24 is fixedly provided at the top of the second end cap 23. The size of the mesh holes of the filter sieve plate 24 is set according to the usage requirements. As a mature prior art, it will not be elaborated here too much.

[0035] The specific implementation scenario is as follows: Pour the mobile phase suspension into the column tube 21, and rinse the inner wall of the column tube 21 with a buffered hydrochloric acid solution to avoid the residue of the suspension on the inner wall of the column tube 21. Inject the mobile phase test solution into the cavity 411, and then start the second linear actuator 33. The output shaft of the second linear actuator 33 drives the piston 41 to move vertically into the column tube 21. At this time, the first through hole 412 and the second through hole 421 are in a misaligned state, that is, the cavity 411 is not communicated with the outside. Then install the first end cap 22 on the top of the column tube 21. At this time, the suspension is located between the bottom of the piston 41 and the filter sieve plate 24 in the column tube 21. Let the piston 41 continue to move downward in the column tube 21. The downward movement of the piston 41 will generate pressure on the suspension between the piston 41 and the filter sieve plate 24. The excess liquid in the suspension will be filtered through the filter sieve plate 24 and discharged through the liquid discharge port. After the excess liquid in the suspension is discharged, the remaining granular packing, that is, the stationary phase, the piston 41 squeezing the stationary phase can ensure that the stationary phase is uniformly and tightly packed in the column tube 21, preventing it from moving or deforming when the mobile phase passes through, and the piston 41 always applies a constant pressure to the stationary phase. Then start the pump. The pump transports the mobile phase into the cavity 411 and drives the rotating disk 42 to rotate. The rotation of the rotating disk 42 makes the second through hole 421 communicate with the first through hole 412. The mobile phase liquid in the cavity 411 is discharged onto the stationary phase through the first through hole 412. Since there are multiple first through holes 412, the mobile phase is discharged onto the surface of the stationary phase through multiple different first through holes 412, and the mobile phase undergoes chromatography separation when passing through the stationary phase.

[0036] In the prior art, a mobile phase delivery pipe 43 is provided at the bottom of a piston 41. There are two ways to drive the piston 41 to compress the stationary phase in the column tube 21. The first way is that when driving the piston 41 to compress the column tube 21, in order to prevent the liquid in the stationary phase from entering the bottom end of the mobile phase delivery pipe 43, a pump is needed to continuously inject gas into the piston 41 to apply pressure, so as to ensure the balance of the pressure generated by the piston 41 moving downward in the column tube 21 on the bottom of the column tube 21. When the stationary phase is compacted, the pump then delivers the mobile phase so that the mobile phase enters the column tube 21. The second way is to install a gate valve at the bottom end of the mobile phase delivery pipe 43. When the piston 41 moves downward in the column tube 21 to apply pressure to the stationary phase, the gate valve is in a closed state. When the stationary phase is compacted, the gate valve is then opened to allow the mobile phase to enter the column tube 21. However, in the first method, there may be a risk of gas mixing into the stationary phase, thus damaging the structure of the stationary phase and affecting the separation effect. In the second method, some particles in the stationary phase may enter the pipeline and contact the valve core of the gate valve. When the valve core rotates, due to the existence of fine particles in the stationary phase, the valve core is worn and finally the gate valve leaks. Both of the two implementation methods in the prior art have disadvantages.

[0037] Compared with the prior art, in this solution, when the piston 41 moves downward in the column tube 21 and applies pressure to the suspension, the first through hole 412 and the second through hole 421 are misaligned, that is, the cavity 411 is in a closed state with the outside. The bottom of the piston 41 can apply a stable pressure to the stationary phase, ensuring that the stationary phase can be evenly and tightly filled in the column tube 21. After the stationary phase is compacted by the constant pressure applied by the piston 41, the rotating disk 42 is driven to rotate, and the second through hole 421 is communicated with the first through hole 412. The mobile phase liquid is discharged onto the stationary phase through a plurality of first through holes 412 evenly arranged at equal intervals. Especially when the diameter of the column tube 21 is large, the dispersed first through holes 412 can distribute the mobile phase more evenly, reduce the impact on the stationary phase, and enable the mobile phase to fully participate in the separation process, so that different components can be well separated, improving the separation efficiency.

[0038] Refer to the accompanying Figures 7 to 11, since a cavity 411 is provided in the piston 41 for storing the mobile phase, in order to make the bottom of the piston 41 have greater strength and avoid deformation when the bottom of the piston 41 applies pressure to the stationary phase, a relatively large wall thickness is required between the bottom of the piston 41 and the inner wall of the bottom of the cavity 411 to enhance the overall strength of the piston 41, that is, the through hole 412 needs to have a relatively large length. Especially in the application of a high-pressure liquid chromatography column, preferably, for example, the length of the through hole 412 is set to 2 to 3 centimeters. When the length of the through hole 412 is relatively large, the bottom of the piston 41 applies a downward extrusion force to the stationary phase, which will cause some packing particles in the stationary phase to adhere to the inner wall of the through hole 412 and contact the bottom of the rotating disk 42. When it is necessary to connect the through hole 412 with the through hole 421, the packing particles attached to the bottom of the through hole 412 will rotate together with the bottom of the rotating disk 42, resulting in wear on the bottom of the rotating disk 42. The wear on the bottom of the rotating disk 42 will cause damage to the overall sealing performance of the rotating disk 42 and produce scratches. Moreover, the wear on the bottom of the rotating disk 42 will also allow the mobile phase to enter the scratches, causing residue of the mobile phase, which is not conducive to subsequent cleaning. Since the diameter of the column tube 21 is relatively large and the number of through holes 412 provided at the bottom of the piston 41 is relatively large, some packing particles in the stationary phase will also enter the cavity 411. The reduction of the packing particles in the stationary phase will lead to a decrease in column efficiency. To avoid the above situation, specifically, a material removing mechanism 5 is provided in the piston 41. The material removing mechanism 5 includes a movable shaft 51. An arc-shaped hole 52 is provided on the rotating disk 42. The movable shaft 51 is slidably arranged in the arc-shaped hole 52 and the through hole 412. A guide groove 521 is provided on the inner wall of the arc-shaped hole 52. A connecting seat 511 is fixedly arranged on the movable shaft 51. The connecting seat 511 is slidably arranged in the guide groove 521. A through hole 54 is provided on the rotating disk 42. A through hole 53 is provided on the inner wall of the bottom of the cavity 411. The through hole 53 is adapted to the through hole 54. The bottom end of the through hole 53 is communicated with the through hole 412. The bottom end of the through hole 53 is also adapted to the bottom end of the movable shaft 51. A through hole 522 is provided on the arc-shaped hole 52. The through hole 522 is communicated with the through hole 54. The through hole 522 is used to transport the mobile phase in the arc-shaped hole 52 to the through hole 54.

[0039] It should be noted that the aperture of the through hole 53 in the figure is only a schematic diagram and does not represent the difference in diameter between the through hole 53 and the through hole 412. The aperture of the through hole 53 can be increased to meet the use requirements. As a mature prior art, it will not be elaborated here too much.

[0040] It should also be noted that the radian of the arc-shaped hole 52 is concentrically arranged with the rotating disk 42, and the depth of the guide groove 521 on the inner wall of the arc-shaped hole 52 is concentrically arranged with the radian of the arc-shaped hole 52, that is, when the rotating disk 42 rotates, the distance between the connecting seats 511 in the guide groove 521 is always the same. The setting of the through hole 522 can avoid the residue of the mobile phase in the arc-shaped hole 52.

[0041] In this embodiment, when the piston 41 vertically moves downward in the column tube 21 to apply pressure to the stationary phase, the bottom end of the movable shaft 51 is flush with the bottom of the piston 41. The granular packing in the stationary phase contacts the bottom of the piston 41 and the bottom end of the movable shaft 51. When the stationary phase is compacted and the mobile phase in the cavity 411 needs to be introduced into the column tube 21, the rotating disk 42 is driven to rotate. When the rotating disk 42 rotates, under the guiding action of the guiding groove 521, the movable shaft 51 can vertically move upward in the through hole 412. At this time, a little granular packing may adhere to the bottom end of the movable shaft 51. Refer to Figure 8 And Figure 9 , with the continuous upward movement of the movable shaft 51, in the vertical direction, the through hole 54 also gradually approaches the corresponding through hole 53. When the bottom end of the movable shaft 51 and the bottom end of the through hole 53 are at the same horizontal height, or when the upward movement of the movable shaft 51 causes the through hole 412 to communicate with the bottom end of the through hole 53, at this time, the through hole 54 also communicates with the through hole 53. The mobile phase in the cavity 411 can flow into the stationary phase through the cooperation of the through hole 54, the through hole 53, and the through hole 412. And the mobile phase discharged from the bottom end of the through hole 53 can wash the packing particles adhering to the bottom end of the movable shaft 51, preventing the packing particles from entering the cavity 411, and the packing particles will not cause wear to the movable shaft 51 or the rotating disk 42, thereby ensuring the column efficiency of the chromatographic column and improving the separation efficiency. After the rotating disk 42 rotates in the reverse direction, the movable shaft 51 resets.

[0042] Refer to the attached Figures 1 to 3 In the specification, in order to facilitate the removal of the column tube 21 and the cleaning of the inner wall of the column tube 21, specifically, two support plates 11 are fixedly arranged on the support frame 1. Installation holes are formed in the support plates 11. An auxiliary ring is fixedly arranged on the support plates 11. The auxiliary ring is concentric with the installation hole. Fixed rings 12 are placed in both auxiliary rings. The diameter of the fixed ring 12 is larger than the diameter of the installation hole. Two connecting rings 211 are fixedly arranged on the column tube 21. The fixed ring 12 and the corresponding connecting ring 211 are fixed by bolts.

[0043] It should be noted that the fixed ring 12 includes two semi-circular rings, and the two semi-circular rings can form a complete fixed ring 12, that is, the fixed ring 12 is a complete ring composed of two semi-circular rings. The diameter of the connecting ring 211 is smaller than the diameter of the installation hole.

[0044] It should also be noted that when the column tube 21 needs to be installed on the support frame 1, first, the column tube 21 is placed into the installation hole, then the fixed ring 12 is placed into the installation hole, and then the fixed ring 12 is placed into the auxiliary ring. The fixed ring 12 and the connecting ring 211 are fixed by bolts. When the fixed ring 12 and the connecting ring 211 are fixed, the column tube 21 is fixed on the support frame 1.

[0045] Refer to the attached instructions Figures 1 to 4 Figures 1 to 4 , for the convenience of subsequent disassembly of the column tube 21, specifically, the extrusion mechanism 3 further includes a first linear driver 31, the first linear driver 31 is fixedly arranged on the support frame 1, a fixed seat 32 is fixedly arranged on the output end of the first linear driver 31, and a second linear driver 33 is fixedly installed on the fixed seat 32.

[0046] It should be noted that the first linear driver 31 is set as a hydraulic cylinder, the hydraulic cylinder is arranged on the support plate 11, and the fixed seat 32 is fixedly arranged on the output end of the hydraulic cylinder. The fixed seat 32 is also set as a hydraulic cylinder, and the piston 41 is fixedly arranged on the output end of the hydraulic cylinder.

[0047] It should also be noted that when the first linear driver 31 is started, the movement of the output end of the first linear driver 31 drives the fixed seat 32 to move vertically, and the movement of the fixed seat 32 can adjust the height of the second linear driver 33. When disassembling the column tube 21, increasing the height of the fixed seat 32 can facilitate the disassembly of the column tube 21. When the second linear driver 33 is started, the movement of the output end of the second linear driver 33 drives the piston 41 to move along the length direction of the column tube 21 inside the column tube 21.

[0048] Refer to the attached instructions Figures 4 to 8 Figures 4 to 8 , for the convenience of driving the rotating disc 42 to rotate, so as to block the bottom of the piston 41 or release the blockage of the bottom of the piston 41, specifically, a rotating driver 44 is fixedly arranged on the piston 41, the output end of the rotating driver 44 is fixedly arranged with the center point of the rotating disc 42, a connecting hole is formed on the piston 41, a first sealing ring is fixedly arranged on the inner wall of the connecting hole, and the output end of the rotating driver 44 is rotatably arranged in the sealing ring. A second sealing ring is fixedly sleeved on the outer side of the piston 41, and the second sealing ring is in close fit with the inner wall of the column tube 21.

[0049] It should be noted that the rotating driver 44 is set as a motor, the motor is fixedly arranged on the piston 41, and the output shaft of the motor is fixedly arranged with the rotating disc 42.

[0050] It should also be noted that when the rotating driver 44 is started, the rotation of the output end of the rotating driver 44 drives the rotating disc 42 to rotate, and the rotating driver 44 can also apply pressure to the rotating disc 42 so that the rotating disc 42 is always in close fit with the inner wall of the bottom of the cavity 411.

[0051] The above embodiments only represent several implementation manners of the present invention, and the description is relatively specific and detailed, but it should not be construed as a limitation to the scope of the patent of the present invention. It should be pointed out that for those of ordinary skill in the art, without departing from the concept of the present invention, several deformations and improvements can still be made, and these all belong to the protection scope of the present invention.

Claims

1. A liquid chromatography column chromatography separation device, characterized in that, It includes a support frame (1), on which a chromatographic separation component (2) is provided. The chromatographic separation component (2) includes a column tube (21). A second end cap (23) is provided at the bottom end of the column tube (21), and a filter sieve plate (24) is provided at the top of the second end cap (23). The stationary phase is poured into the column tube (21) through the top end of the column tube (21), and the filter sieve plate (24) is used to filter the stationary phase. An extrusion mechanism (3) is provided on the support frame (1). The extrusion mechanism (3) includes a second linear driver (33). A column pressure maintaining mechanism (4) is provided at the output end of the second linear driver (33). The column pressure maintaining mechanism (4) includes a piston (41). A cavity (411) is formed inside the piston (41), and a first through hole (412) is provided at the bottom of the piston (41). A rotating disk (42) is rotatably provided on the bottom inner wall of the cavity (411), and a second through hole (421) is provided on the rotating disk (42). A mobile phase delivery pipe (43) is fixedly communicated inside the cavity (411), and the mobile phase delivery pipe (43) is used to deliver the mobile phase into the cavity (411). The output end of the second linear driver (33) is used to drive the piston (41) to move along the length direction of the column tube (21) inside the column tube (21). When the first through hole (412) and the second through hole (421) are misaligned, the piston (41) vertically moves downward inside the column tube (21) to apply a constant pressure to the stationary phase. When the first through hole (412) and the second through hole (421) are communicated, the mobile phase inside the cavity (411) enters the stationary phase to achieve separation.

2. The liquid chromatography column chromatography separation device according to claim 1, wherein: A material removal mechanism (5) is provided inside the piston (41). The material removal mechanism (5) includes a movable shaft (51). An arc-shaped hole (52) is provided on the rotating disk (42). The movable shaft (51) is slidably arranged in the arc-shaped hole (52) and the first through hole (412). A guide groove (521) is provided on the inner wall of the arc-shaped hole (52). A connecting seat (511) is fixedly provided on the movable shaft (51), and the connecting seat (511) is slidably arranged in the guide groove (521).

3. The liquid chromatography column chromatography separation device according to claim 2, characterized in that: A fourth through hole (54) is provided on the rotating disk (42), and a third through hole (53) is provided on the bottom inner wall of the cavity (411). The third through hole (53) is adapted to the fourth through hole (54). The bottom end of the third through hole (53) is communicated with the first through hole (412), and the bottom end of the third through hole (53) is also adapted to the bottom end of the movable shaft (51).

4. The liquid chromatography column chromatography separation device according to claim 3, characterized in that: A fifth through hole (522) is provided on the arc-shaped hole (52), and the fifth through hole (522) is communicated with the fourth through hole (54). The fifth through hole (522) is used to deliver the mobile phase inside the arc-shaped hole (52) into the fourth through hole (54).

5. The liquid chromatography column chromatography separation device according to claim 4, characterized in that: Two support plates (11) are fixedly arranged on the support frame (1). Mounting holes are formed in the support plates (11). Auxiliary rings are fixedly arranged on the support plates (11). The auxiliary rings are concentric with the mounting holes. Fixing rings (12) are placed in both of the auxiliary rings. The diameter of the fixing ring (12) is larger than the aperture of the mounting hole. Two connecting rings (211) are fixedly arranged on the column tube (21). The fixing ring (12) and the corresponding connecting ring (211) are fixed by bolts.

6. The liquid chromatography column chromatography separation device according to claim 5, characterized in that: The chromatography separation component (2) further includes a first end cover (22). The first end cover (22) is fixedly installed at the top end of the column tube (21). An exhaust hole is formed in the first end cover (22). The exhaust hole is communicated with the column tube (21). The output end of the linear actuator two (33) is slidably arranged with the first end cover (22). A groove is formed in the output end of the linear actuator two (33). The mobile phase delivery tube (43) is located in the groove. One end of the mobile phase delivery tube (43) far from the cavity (411) is fixedly communicated with a pump.

7. The liquid chromatography column chromatography separation device according to claim 6, characterized in that: The extrusion mechanism (3) further includes a linear actuator one (31). The linear actuator one (31) is fixedly arranged on the support frame (1). A fixing seat (32) is fixedly arranged on the output end of the linear actuator one (31). The linear actuator two (33) is fixedly installed on the fixing seat (32).

8. A liquid chromatography column chromatography separation device according to claim 7, characterized in that: A rotation actuator (44) is fixedly arranged on the piston (41). The output end of the rotation actuator (44) is fixedly arranged with the center point of the rotating disc (42). A connection hole is formed in the piston (41). A first sealing ring is fixedly arranged on the inner wall of the connection hole. The output end of the rotation actuator (44) is rotatably arranged in the sealing ring.

9. The liquid chromatography column chromatography separation device according to claim 8, characterized in that: A liquid discharge port is formed in the second end cover (23). The liquid discharge port is located at the center of the second end cover (23). The liquid discharge port is used for discharging the liquid in the column tube (21).

10. A liquid chromatography column chromatography separation device according to claim 9, characterized in that: A second sealing ring is fixedly sleeved on the outer side of the piston (41). The second sealing ring is in close fit with the inner wall of the column tube (21).

Images