Multistage centrifugal equipment for preparing tumor radiotherapy resistant cell strains

Abstract

The invention discloses multi-stage centrifugal equipment for preparing tumor radiotherapy resistant cell strains, which comprises a base, a bearing disc arranged on the base and an upper pressure disc arranged on a mounting frame, and the bearing disc is also provided with a multi-stage oscillation centrifugal mechanism corresponding to the upper pressure disc; the multi-stage oscillation centrifugal mechanism comprises a centrifugal disc, the centrifugal disc is arranged on the bearing disc, a driving shaft is arranged at the bottom of the centrifugal disc, a gear ring is arranged on the surface of one side in an annular groove, a plurality of sets of acceleration centrifugal assemblies are evenly arranged on the centrifugal disc, and the acceleration centrifugal assemblies are connected with the gear ring in a meshed mode; the upper pressing disc is further provided with multiple sets of pressing mechanisms corresponding to the accelerated centrifugal assemblies, and the inner bottom of the annular groove is further provided with an oscillation assembly used for enabling the test tubes to longitudinally move along with rotation of the accelerated centrifugal assemblies, multi-stage centrifugal force can be provided, the centrifugal separation efficiency of tumor radiotherapy resistant cell strains is improved, rapid longitudinal oscillation can be achieved, and the efficiency of tumor radiotherapy resistant cell strains is improved. Meanwhile, the stability and the sealing performance of the test tube in the centrifugation process are improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of medical devices, and particularly relates to a multi-stage centrifugation device for preparing tumor radiotherapy-resistant cell lines. Background Art

[0002] The gastric cancer radiotherapy-resistant strain is a gastric cancer cell line that is resistant to radiation. It is established by irradiating the parental gastric cancer cell line with a small dose and a long cycle, and is of great significance for the study of the relevant mechanisms of gastric cancer radiotherapy. During the establishment of the gastric cancer radiotherapy-resistant strain, a large number of cells will die. In order to separate the dead cells from the surviving cells, continue irradiating and culturing the remaining surviving cells, a centrifugation device is often needed to perform centrifugation separation on them.

[0003] For example, the centrifugation device for preparing gastric cancer radiotherapy-resistant cell lines disclosed in the utility model with the publication number CN213078810U includes a centrifugation mechanism and a power mechanism for centrifuging medicaments. The centrifugation mechanism is arranged in front of the power mechanism, and also includes a fixing mechanism and a supporting mechanism for fixing components. The fixing mechanism includes a lifting plate, a fixing frame, and a rotating seat. The rear end of the lifting plate is arranged on the fixing frame, and the upper end of the rotating seat is connected to the front end of the lifting plate through a bearing. This solution uses the supporting seat on the supporting plate to support the test tube cylinders on the entire centrifugation plate, so that the centrifugation plate and the test tube cylinders rotating at high speed can be fixed, thereby solving the problem of centrifugal vibration of a single sample. By the elastic force of the spring, the top of the supporting seat is pulled downward, so that the lower end of the supporting seat extends out from the inner round hole of the supporting plate, and then the empty test tube cylinders without inserted samples can be supported and fixed.

[0004] The above solution only has a centrifugation function and cannot oscillate the cell suspension in the test tube, so that the cell suspension cannot be evenly distributed, which easily causes sample deviation after centrifugation. Moreover, it uses a single-stage centrifugal force to centrifuge the gastric cancer radiotherapy-resistant cell line, and it cannot perform multi-stage accelerated centrifugation on the gastric cancer radiotherapy-resistant cell line, reducing the centrifugation separation efficiency of the gastric cancer radiotherapy-resistant cell line. At the same time, when installing the test tube, it cannot automatically position and automatically clamp and fix the test tube, and the test tube is likely to move during centrifugation, affecting the centrifugation effect. Therefore, we propose a multi-stage centrifugation device for preparing tumor radiotherapy-resistant cell lines. Summary of the Invention

[0005] The object of the present invention is to provide a multi-stage centrifugation device for preparing tumor radiotherapy-resistant cell lines, so as to solve the problems in the prior art solutions mentioned in the above background technology that the cell suspension in the test tube cannot be shaken, the cell suspension cannot be evenly distributed, which is likely to cause sample deviation after centrifugation, and a single-stage centrifugal force is used to centrifuge the gastric cancer radiotherapy-resistant cell line, which cannot perform multi-stage accelerated centrifugation on the gastric cancer radiotherapy-resistant cell line, reducing the centrifugation separation efficiency of the gastric cancer radiotherapy-resistant cell line. At the same time, when installing the test tube, it cannot automatically position and automatically clamp and fix the test tube, and the test tube is likely to move during centrifugation, affecting the centrifugation effect.

[0006] To achieve the above object, the present invention provides the following technical solution: A multi-stage centrifugation device for preparing tumor radiotherapy-resistant cell lines, including a machine base, a bearing plate arranged on the machine base, a frame arranged on one side of the machine base, a liftable mounting frame arranged on one side of the frame, and an upper pressing plate arranged on the mounting frame. A multi-stage oscillation centrifugation mechanism corresponding to the upper pressing plate is also arranged on the bearing plate;

[0007] The multi-stage oscillation centrifugation mechanism includes a centrifugal disk. The centrifugal disk is arranged on the bearing plate. A driving shaft is arranged at the bottom of the centrifugal disk. The driving shaft is rotatably arranged in the middle of the bearing plate and is connected to the output shaft of a driving motor located in the machine base. A ring groove is arranged on the upper side surface of the bearing plate. A toothed ring is arranged on one surface of the inner side of the ring groove. A plurality of groups of acceleration centrifugation components are evenly arranged on the centrifugal disk. The acceleration centrifugation components are meshed and connected with the toothed ring. An oscillation component for the test tube to move longitudinally as the acceleration centrifugation component rotates is also arranged at the bottom of the ring groove;

[0008] Among them, a plurality of groups of pressing mechanisms corresponding to the acceleration centrifugation components are also arranged on the upper pressing plate.

[0009] Preferably, the acceleration centrifugation component includes a bearing sleeve. The bearing sleeve is rotatably arranged in an upper sleeve. The upper sleeve is rotatably arranged on the centrifugal disk. A lower sleeve is arranged at the bottom of the bearing sleeve. The lower sleeve is rotatably arranged in a mounting seat. The mounting seat is arranged in the ring groove. Upper longitudinal sliders are also arranged on both sides of the bearing sleeve. The upper longitudinal sliders are slidably arranged in upper longitudinal chutes. The upper longitudinal chutes are symmetrically arranged on both sides inside the upper sleeve. A first spring is also arranged on one side inside the upper longitudinal chute. One end of the first spring is connected to the upper longitudinal slider, which improves the stability of the bearing sleeve during rotation and can guide the longitudinal movement of the bearing sleeve during the oscillation process.

[0010] Preferably, a gear groove is arranged on one side inside the mounting seat. A transmission gear is rotatably arranged in the gear groove. The transmission gear is meshed with the toothed ring. Through the transmission of the transmission gear and the toothed ring, the transmission gear rotates counterclockwise during the rotation of the centrifugal disk.

[0011] Preferably, the transmission gear meshes with the acceleration gear. The acceleration gear is arranged on the lower side of the lower sleeve. The outer side of the acceleration gear is arranged in the receiving groove, and the receiving groove communicates with the gear groove. Both sides inside the lower sleeve are provided with lower longitudinal chutes, and lower longitudinal sliders are slidably arranged in the lower longitudinal chutes. One end of the lower longitudinal slider is connected to the bearing sleeve. When the transmission gear rotates, it can drive the acceleration gear to rotate clockwise, thereby enabling the bearing sleeve to rotate clockwise at an accelerated speed, further increasing the centrifugal force. At the same time, during the oscillation of the bearing sleeve, it can guide the movement of the lower part of the bearing sleeve.

[0012] Preferably, a longitudinal guide block is arranged on one side of the bottom of the mounting seat. One end of the longitudinal guide block is slidably arranged in the lower annular chute, and the lower annular chute is arranged at the bottom of the annular groove. When the mounting seat rotates, the longitudinal guide block moves along the lower annular chute, which can guide the movement of the mounting seat.

[0013] Preferably, the oscillation assembly includes a wavy support ring. The wavy support ring is arranged in the bottom groove body, and the bottom groove body is arranged at the bottom of the annular groove. A hydraulic cylinder is also arranged in the bottom groove body. One end of the piston rod of the hydraulic cylinder is connected to the wavy support ring. The wavy support ring is in contact with the bottom of the bearing sleeve. When multiple bearing sleeves rotate, it can automatically perform longitudinal oscillation.

[0014] Preferably, a transverse guide block is arranged on one side surface of the mounting seat. One end of the transverse guide block is slidably arranged in the upper annular chute, and the upper annular chute is arranged on one side surface of the annular groove. When the mounting seat rotates, the transverse guide block moves in the upper annular chute, further improving the guiding property when the mounting seat rotates.

[0015] Preferably, the pressing mechanism includes an upper pressing sleeve. The upper pressing sleeve is rotatably arranged at the bottom of the upper pressing plate. The upper pressing sleeve corresponds to the bearing sleeve. The upper pressing plate is rotatably arranged on the mounting frame through a bearing shaft, which can enable the upper pressing plate to rotate, and at the same time, the upper pressing sleeve can rotate within the upper pressing plate.

[0016] Preferably, an upper support sleeve is movably arranged inside the upper pressing sleeve. An upper pressing plate is also arranged inside the upper support sleeve. A conical sealing pad is further arranged at the bottom of the upper pressing plate. Positioning blocks are also arranged on both sides of the upper support sleeve. One end of the positioning block is slidably arranged in the bearing groove. A second spring is also arranged on one side surface inside the bearing groove. One end of the second spring is connected to the positioning block. When the upper pressing sleeve presses the test tube, it can perform sealed pressing on test tubes with different diameters and can guide the movement of the end of the test tube.

[0017] Preferably, a bottom support is further provided at the inner bottom of the bearing sleeve. Limit blocks are provided on both sides of the bottom support. One end of each limit block is slidably arranged in a limit groove. The limit grooves are arranged on both sides inside the bearing sleeve. Lower springs are further arranged in the limit grooves. One end of each lower spring is connected to the corresponding limit block. Groove bodies are also provided on both sides inside the bearing sleeve. Clamping and positioning components that move along with the limit blocks are arranged in the groove bodies. When a test tube is placed, the elastic force of the lower springs can buffer the pressure on the test tube and protect the bottom of the test tube. At the same time, after centrifugation is completed, the elastic force of the lower springs can automatically lift the test tube, facilitating the taking and placing of the test tube.

[0018] Preferably, the clamping and positioning components include lower ear seats. The lower ear seats are arranged on one side of the limit blocks. Pulling rods are rotatably arranged in the lower ear seats. One end of each pulling rod is rotatably connected to an upper ear seat. The upper ear seats are arranged on one side of the clamping arms. The clamping arms are rotatably arranged in the groove bodies. One end of each clamping arm is connected to a fixing pad. Grooves are further arranged on one side surface of the fixing pad. Upper springs are arranged in the grooves. One end of each upper spring is connected to a positioning pin. The positioning pins are movably arranged in the grooves. When a test tube is placed, it can be automatically positioned, facilitating the precise pressing of the test tube by the upper pressing sleeve. At the same time, when the upper pressing sleeve presses down, it can automatically clamp and fix the test tube, improving the stability during the centrifugation of the test tube.

[0019] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0020] (1) During the rotation of the centrifugal disc in this application, the bearing sleeve can be driven to revolve and centrifuge along the center of the bearing disc. At the same time, under the driving action of the gear ring, the bearing sleeve can also rotate rapidly along its axis, thereby providing multi-stage centrifugal force. At the same time, during the revolution and rotation of the bearing sleeve, it can vibrate longitudinally rapidly, facilitating the rapid and uniform distribution of the cell suspension. At the same time, it greatly improves the centrifugal separation efficiency of tumor radiotherapy-resistant cell lines, combines the vibration and centrifugation processes into one, and improves the preparation efficiency of tumor radiotherapy-resistant cell lines.

[0021] (2) During the use of this application, primary positioning of the test tube can be carried out. At the same time, during the pressing process, the test tube can be automatically sealed and clamped and fixed on the outer surface of the test tube, improving the stability and sealing performance during the centrifugation of the test tube. And after the preparation is completed, it can be automatically loosened, facilitating the taking and placing of the test tube. Description of the Drawings

[0022] Figure 1 is the overall structural schematic diagram of the present invention;

[0023] Figure 2 is the half-sectional structural schematic diagram of the multi-stage oscillating centrifugation mechanism in the present invention;

[0024] Figure 3 isFigure 2 Schematic diagram of the enlarged structure at A in the present invention;

[0025] Figure 4 Top view sectional structure schematic diagram of the multi-stage oscillating centrifugal mechanism in the present invention;

[0026] Figure 5 Schematic diagram of the structure of the wavy support ring in the present invention;

[0027] Figure 6 Overall structure schematic diagram of the upper pressure plate in the present invention;

[0028] Figure 7 Half-sectional structure schematic diagram of the upper pressure plate in the present invention;

[0029] Figure 8 Half-sectional structure schematic diagram of the upper pressure sleeve in the present invention;

[0030] Figure 9 Half-sectional structure schematic diagram of the bearing sleeve in the present invention;

[0031] Figure 10 is Figure 7 Schematic diagram of the enlarged structure at B in the present invention;

[0032] Figure 11 Schematic diagram of the structure of the clamping and positioning assembly in the present invention;

[0033] Figure 12 Half-sectional structure schematic diagram of the fixing pad in the present invention;

[0034] Figure 13 Half-sectional structure schematic diagram of the bearing sleeve during the test tube pressing process in the present invention;

[0035] In the figure: 1. Multi-stage oscillating centrifugal mechanism; 2. Upper pressing plate; 3. Mounting frame; 4. Frame; 5. Pressing mechanism; 6. Machine base; 7. Control panel; 8. Carrier plate; 9. Electric push rod; 10. Test tube; 21. Carrier shaft; 101. Driving motor; 102. Driving shaft; 103. Annular groove; 104. Centrifugal disc; 105. Carrier sleeve; 106. Mounting seat; 107. Gear ring; 108. Transmission gear; 109. Gear groove; 110. Accelerating gear; 111. Storage groove; 112. Lateral guide block; 113. Lower annular chute; 114. Longitudinal guide block; 115. Upper annular chute; 116. Hydraulic cylinder; 117. Wavy support ring; 118. Lower longitudinal slider; 119. Lower sleeve; 120. Upper sleeve; 121. First spring; 122. Upper longitudinal slider; 501. Upper pressing sleeve; 502. Conical sealing gasket; 503. Upper pressing plate; 504. Bottom support; 505. Arc-shaped positioning groove; 506. Pull rod; 507. Adjusting port; 508. Rotating shaft; 509. Clamping arm; 510. Fixed pad; 511. Tank body; 512. Lower ear seat; 513. Limit block; 514. Lower spring; 515. Limit groove; 516. Upper ear seat; 517. Arc-shaped surface; 518. Positioning pin; 519. Groove; 520. Upper spring; 521. Upper support sleeve; 522. Second spring; 523. Positioning block. Detailed implementation manner

[0036] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0037] Please refer to Figures 1 - 5 , the present invention provides a technical solution: a multi-stage centrifugal device for preparing tumor radiotherapy-resistant cell lines. A carrier plate 8 is fixed on the upper surface of the machine base 6, and a frame 4 is fixedly arranged on the rear side surface of the machine base 6. A liftable mounting frame 3 is arranged on one side of the frame 4. Specifically, an electric push rod 9 is also arranged in the frame 4, and one end of the piston rod of the electric push rod 9 is connected to the mounting frame 3, which can adjust the height of the mounting frame 3. An upper pressing plate 2 is arranged on the mounting frame 3, and a multi-stage oscillating centrifugal mechanism 1 corresponding to the upper pressing plate 2 is also arranged on the carrier plate 8;

[0038] The multi-stage oscillating centrifugal mechanism 1 includes a centrifugal disk 104. The centrifugal disk 104 is arranged above the bearing disk 8. A drive shaft 102 is provided at the bottom of the centrifugal disk 104. The drive shaft 102 is rotatably arranged in the middle of the bearing disk 8 and is connected to the output shaft of a drive motor 101 located in the machine base 6. The drive motor 101 can drive the drive shaft 102 to rotate. The rotation of the drive shaft 102 can drive multiple sets of bearing sleeves 105 to revolve around the center of the bearing disk 8 and revolve the test tubes 10. An annular groove 103 is provided on the upper surface of the bearing disk 8. A gear ring 107 is provided on one side surface of the annular groove 103. Multiple sets of acceleration centrifugal components are evenly arranged on the centrifugal disk 104. The acceleration centrifugal components are meshed and connected with the gear ring 107; it can drive the bearing sleeve 105 to rotate while revolving, thereby increasing the centrifugal force.

[0039] The acceleration centrifugal component includes a bearing sleeve 105. The bearing sleeve 105 is rotatably arranged in an upper sleeve 120. The upper sleeve 120 is rotatably arranged on the centrifugal disk 104. A lower sleeve 119 is provided at the bottom of the bearing sleeve 105. The lower sleeve 119 is rotatably arranged in a mounting seat 106. The mounting seat 106 is arranged in the annular groove 103. Upper longitudinal sliders 122 are also provided on both sides of the bearing sleeve 105. The upper longitudinal sliders 122 are slidably arranged in upper longitudinal chutes. The upper longitudinal chutes are symmetrically arranged on both sides inside the upper sleeve 120. A first spring 121 is also provided on one side inside the upper longitudinal chute. One end of the first spring 121 is connected to the upper longitudinal slider 122, which can enable the bearing sleeve 105 to rotate synchronously in the centrifugal disk 104 and the mounting seat 106, improving the stability of the bearing sleeve 105 during rotation; a gear groove 109 is provided on one side inside the mounting seat 106. A transmission gear 108 is rotatably arranged in the gear groove 109. The transmission gear 108 is meshed with the gear ring 107. Through the transmission of the transmission gear 108 and the gear ring 107, during the rotation of the centrifugal disk 104, the transmission gear 108 is driven to rotate counterclockwise.

[0040] The transmission gear 108 is meshed with an acceleration gear 110. Specifically, the diameter size of the transmission gear is larger than the diameter size of the acceleration gear 110, which can improve the rotation speed of the bearing sleeve 105. The acceleration gear 110 is arranged below the lower sleeve 119. The outer side of the acceleration gear 110 is arranged in a receiving groove 111. The receiving groove 111 communicates with the gear groove 109. Lower longitudinal chutes are provided on both sides inside the lower sleeve 119. Lower longitudinal sliders 118 are slidably arranged in the lower longitudinal chutes. One end of the lower longitudinal slider 118 is connected to the bearing sleeve 105. When the transmission gear 108 rotates, it can drive the acceleration gear 110 to rotate clockwise, thereby enabling the bearing sleeve 105 to rotate clockwise at an accelerated speed, thereby increasing the centrifugal force.

[0041] An oscillation component for the longitudinal movement of the test tube as it rotates with the acceleration and centrifugation component is also provided at the inner bottom of the annular groove 103; the oscillation component includes a wavy support ring 117, the wavy support ring 117 is arranged in the bottom groove body, the bottom groove body is arranged at the inner bottom of the annular groove 103, a hydraulic cylinder 116 is also arranged in the bottom groove body, one end of the piston rod of the hydraulic cylinder 116 is connected to the wavy support ring 117, the wavy support ring 117 fits against the bottom of the bearing sleeve 105, and when multiple groups of bearing sleeves rotate, longitudinal oscillation can be automatically performed.

[0042] First, place the test tube 10 containing the tumor radiotherapy-resistant cell line in the bearing sleeve 105, then drive the mounting frame 3 to descend through the electric push rod 9, the mounting frame 3 drives the upper pressing plate 2 to descend, the upper pressing plate 2 drives the pressing mechanism 5 to descend, and the test tube 10 is pressed and positioned through the pressing mechanism 5. Then, drive the drive shaft 102 to rotate clockwise with low power by the drive motor 101, the drive shaft 102 drives the centrifugal disc 104 to rotate clockwise, the rotation of the centrifugal disc 104 drives the mounting seat 106 to rotate clockwise in the annular groove 103, when the centrifugal disc 104 rotates, it drives the bearing sleeve 105 to revolve around the center of the bearing disc 8. At the same time, when the mounting seat 106 rotates, it drives the transmission gear 108 to rotate along the toothed ring 107. Through the meshing of the toothed ring 107 and the transmission gear 108, the transmission gear 108 rotates counterclockwise, the transmission gear 108 drives the acceleration gear 110 to rotate clockwise and accelerate, the clockwise acceleration rotation drives the lower sleeve 119 to rotate, the rotation of the lower sleeve 119 drives the bearing sleeve 105 to rotate clockwise and accelerate, and at the same time the bearing sleeve 105 drives the upper sleeve 120 to rotate. The bearing sleeve 105 rotates clockwise and accelerates and rotates within the centrifugal disc 104. When the bearing sleeve 105 revolves and rotates, the bearing sleeve 105 moves along the wavy support ring 117, and thus the bearing sleeve 105 moves longitudinally. The bearing sleeve 105 simultaneously drives the lower longitudinal slider 118 and the upper longitudinal slider 122 to move longitudinally. Through the elastic force of the first spring 121, the bearing sleeve 105 can be reset and move, thereby oscillating the test tube and making the cell suspension evenly distributed in the test tube. After the oscillation is completed, the wavy support ring 117 is moved into the bottom groove body through the hydraulic cylinder 116, and the wavy support ring 117 is separated from the bearing sleeve 105. Then, increase the power of the drive motor 101. Since the rotation directions of the bearing sleeve 105 and the centrifugal disc 104 are the same, their centrifugal force directions are the same. Under the action of the double centrifugal force, the tumor radiotherapy-resistant cell line in the test tube 10 can be quickly separated.

[0043] Further, a longitudinal guide block 114 is provided on one side of the bottom of the mounting seat 106. One end of the longitudinal guide block 114 is slidably disposed in the lower annular chute 113. The lower annular chute 113 is provided at the inner bottom of the annular groove 103. A transverse guide block 112 is provided on one side surface of the mounting seat 106. One end of the transverse guide block 112 is slidably disposed in the upper annular chute 115. The upper annular chute 115 is provided on one side surface of the annular groove 103. When the mounting seat 106 rotates, the longitudinal guide block 114 moves along the lower annular chute 113, and the mounting seat 106 drives the transverse guide block 112 to move in the upper annular chute 115, so that the mounting seat 106 can be limited in movement in both the horizontal and vertical directions, further improving the guiding property when the mounting seat 106 rotates.

[0044] A control panel 7 is further provided on the machine base 6. The control panel 7 can control the telescoping of the electric push rod 9 and the operation of the drive motor 101.

[0045] Please refer to Figures 9 - 13 , a bottom support 504 is further provided at the inner bottom of the bearing sleeve 105. An arc-shaped positioning groove 505 is further provided in the middle of the bottom support 504. When the test tube 10 is placed in the bearing sleeve 105, the bottom of the test tube 10 can be fitted and the test tube 10 can be positioned. Limiting blocks 513 are provided on both sides of the bottom support 504. One end of each limiting block 513 is slidably disposed in a limiting groove 515. The limiting grooves 515 are provided on both sides inside the bearing sleeve 105. Lower springs 514 are further provided in the limiting grooves 515. One end of each lower spring 514 is connected to the corresponding limiting block 513. Grooves 511 are further provided on both sides inside the bearing sleeve 105. Clamping and positioning components that move along with the limiting blocks 513 are provided in the grooves 511. When the test tube 10 is placed, the elasticity of the lower springs 514 can buffer the pressure on the test tube 10 and protect the bottom of the test tube 10. At the same time, the elasticity of the lower springs 514 can automatically lift the test tube 10 after centrifugation is completed, facilitating the taking and placing of the test tube 10.

[0046] The clamping and positioning assembly includes a lower ear seat 512, which is arranged on one side of the limit block 513. A pull rod 506 is rotatably arranged in the lower ear seat 512. One end of the pull rod 506 passes through the adjustment port 507 and is rotatably connected to the upper ear seat 516. The adjustment port 507 connects the groove body 511 and the limit groove 515. The upper ear seat 516 is arranged on one side of the clamping arm 509. The clamping arm 509 is rotatably arranged in the groove body 511 through a rotating shaft 508. One end of the clamping arm 509 is connected to a fixed pad 510. A groove 519 is also arranged on one side surface of the fixed pad 510. An upper spring 520 is arranged in the groove 519. One end of the upper spring 520 is connected to a positioning pin 518. The positioning pin 518 is movably arranged in the groove 519. When the test tube 10 is placed, it can automatically position the test tube 10, facilitating the precise pressing of the test tube 10 by the upper pressing sleeve 501. At the same time, when the upper pressing sleeve 501 presses down, it can automatically clamp and fix the test tube 10, improving the stability of the test tube 10 during the centrifugation process.

[0047] Please refer to Figure 6 and Figure 7 Moreover, the upper pressing plate 2 is also provided with multiple groups of pressing mechanisms 5 corresponding to the acceleration centrifugation assembly. The pressing mechanism 5 includes an upper pressing sleeve 501, which is rotatably arranged at the bottom of the upper pressing plate 2. The upper pressing sleeve 501 corresponds to the bearing sleeve 105. The upper pressing plate 2 is rotatably arranged on the mounting frame 3 through a bearing shaft 21, enabling the upper pressing plate 2 to rotate. At the same time, the upper pressing sleeve 501 can rotate within the upper pressing plate 2.

[0048] When the test tube 10 is placed in the bearing sleeve 105, the test tube 10 is in contact with the bottom support 504. Under the action of gravity, the bottom support 504 drives the limit block 513 to move downward in the limit groove 515. The limit block 513 compresses the lower spring 514, and the lower spring 514 absorbs the pressure. As the limit block 513 moves downward, it drives the lower ear seat 512 to move downward. The downward movement of the lower ear seat 512 drives the pull rod 506 to move downward. The downward movement of the pull rod 506 drives the upper ear seat 516 to move downward. The lower ear seat 512 drives the clamping arm 509 to rotate through the rotating shaft 508. The rotation of the clamping arm 509 drives the fixed pad 510 to rotate, and makes the positioning pin 518 on the fixed pad 510 fit with the outer surface of the test tube 10, thereby automatically positioning the test tube 10 and facilitating the precision of the pressing by the upper pressing sleeve 501.

[0049] When the upper pressing plate 2 drives the upper pressing sleeve 501 to move downward, the upper pressing sleeve 501 drives the upper supporting sleeve 521 to move downward, so that the upper supporting sleeve 521 fits against the end of the test tube 10. As the upper pressing sleeve 501 moves downward, the test tube 10 drives the bottom support 504 to move downward. As the bottom support 504 moves, the limit block 513 drives the lower ear seat 512 to continuously move downward, and the lower ear seat 512 drives the pull rod 506 to move downward. By rotating the clamping arm 509, the fixing pad 510 moves. The movement of the fixing pad 510 causes the positioning pin 518 to move within the groove 519 and compresses the upper spring 520. When the fixing pad 510 fits against the outer surface of the test tube 10, the fixing pad 510 can clamp and fix the test tube 10. When the drive shaft 102 drives the centrifugal disc 104 to rotate, the upper pressing plate 2 rotates synchronously on the mounting bracket 3 through the bearing shaft 21. At the same time, the upper pressing sleeve 501 rotates with the bearing sleeve 105 and rotates within the upper pressing plate 2.

[0050] Furthermore, an arc surface 517 is provided on one side of the fixing pad 510. During the clamping process, the arc surface 517 can fit against the outer surface of the test tube 10, increasing the contact area between the fixing pad 510 and the outer surface of the test tube 10 and further improving the clamping stability.

[0051] Please refer to Figure 8 Furthermore, the upper supporting sleeve 521 is movably arranged within the upper pressing sleeve 501. An upper pressing plate 503 is also arranged within the upper supporting sleeve 521. A conical sealing pad 502 is also provided at the bottom of the upper pressing plate 503. Positioning blocks 523 are also provided on both sides of the upper supporting sleeve 521. One end of the positioning block 523 is slidably arranged within the bearing groove. A second spring 522 is also provided on one side surface within the bearing groove. One end of the second spring 522 is connected to the positioning block 523. When the upper pressing sleeve 501 presses the test tube 10, the conical sealing pad 502 is inserted into the test tube 10 and fits against the inner wall of the test tube 10, enabling sealing and pressing of test tubes 10 with different diameters. At the same time, when the test tube moves longitudinally back and forth, the test tube can drive the upper supporting sleeve 521 to move longitudinally within the upper pressing sleeve 501, and at the same time drive the positioning block 523 to move. The positioning block 523 compresses the second spring 522. Through the elastic force of the second spring 522, the upper supporting sleeve 521 can be reset and move, and at the same time the upper supporting sleeve 521 can be made to fit against the test tube, ensuring the stability during the centrifugation of the test tube.

[0052] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A multi-stage centrifugation device for preparing tumor radiotherapy-resistant cell lines, comprising a machine base (6), a carrier plate (8) arranged on the machine base (6), a frame (4) arranged on one side of the machine base (6), a liftable mounting frame (3) arranged on one side of the frame (4), and an upper pressing plate (2) arranged on the mounting frame (3), characterized in that: A multi-stage oscillating and centrifugal mechanism (1) corresponding to the upper pressing plate (2) is further provided on the carrier plate (8); The multi-stage oscillating and centrifugal mechanism (1) includes a centrifugal disc (104). The centrifugal disc (104) is arranged on the carrier plate (8). A driving shaft (102) is arranged at the bottom of the centrifugal disc (104). The driving shaft (102) is rotatably arranged in the middle of the carrier plate (8) and is connected to the output shaft of a driving motor (101) located in the machine base (6). An annular groove (103) is arranged on the upper side surface of the carrier plate (8). A toothed ring (107) is arranged on one surface of the inner side of the annular groove (103). Multiple groups of acceleration and centrifugal components are evenly arranged on the centrifugal disc (104). The acceleration and centrifugal components are meshed and connected with the toothed ring (107). An oscillating component for the test tube to move longitudinally as the acceleration and centrifugal components rotate is further arranged at the bottom of the annular groove (103); Among them, multiple groups of pressing mechanisms (5) corresponding to the acceleration and centrifugal components are further arranged on the upper pressing plate (2).

2. The multi-stage centrifugation device prepared from the tumor radiotherapy-resistant cell line according to claim 1, characterized in that: The acceleration and centrifugal component includes a bearing sleeve (105). The bearing sleeve (105) is rotatably arranged in an upper sleeve (120). The upper sleeve (120) is rotatably arranged on the centrifugal disc (104). A lower sleeve (119) is arranged at the bottom of the bearing sleeve (105). The lower sleeve (119) is rotatably arranged in a mounting seat (106). The mounting seat (106) is arranged in the annular groove (103). Upper longitudinal sliding blocks (122) are further arranged on both sides of the bearing sleeve (105). The upper longitudinal sliding blocks (122) are slidably arranged in upper longitudinal sliding grooves. The upper longitudinal sliding grooves are symmetrically arranged on both sides inside the upper sleeve (120). A first spring (121) is further arranged on one side inside the upper longitudinal sliding groove. One end of the first spring (121) is connected to the upper longitudinal sliding block (122).

3. The multi-stage centrifugation device prepared from the tumor radiotherapy-resistant cell line according to claim 2, characterized in that: A gear groove (109) is arranged on one side inside the mounting seat (106). A transmission gear (108) is rotatably arranged in the gear groove (109). The transmission gear (108) is meshed with the toothed ring (107).

4. The multi-stage centrifugation device for preparing tumor radiotherapy-resistant cell lines according to claim 3, characterized in that: The transmission gear (108) is meshed with an acceleration gear (110). The acceleration gear (110) is arranged on the lower side of the lower sleeve (119). The outer side of the acceleration gear (110) is arranged in a receiving groove (111). The receiving groove (111) is communicated with the gear groove (109). Lower longitudinal sliding grooves are arranged on both sides inside the lower sleeve (119). Lower longitudinal sliding blocks (118) are slidably arranged in the lower longitudinal sliding grooves. One end of the lower longitudinal sliding block (118) is connected to the bearing sleeve (105).

5. The multi-stage centrifugation device prepared from the tumor radiotherapy-resistant cell line according to claim 2 or 3, characterized in that: A longitudinal guiding block (114) is arranged on one side of the bottom of the mounting seat (106). One end of the longitudinal guiding block (114) is slidably arranged in a lower annular sliding groove (113). The lower annular sliding groove (113) is arranged at the bottom of the annular groove (103).

6. The multi-stage centrifugation device prepared from the tumor radiotherapy-resistant cell line according to claim 1, characterized in that: The oscillation assembly includes a wavy support ring (117) which is arranged in the bottom groove body. The bottom groove body is arranged at the inner bottom of the annular groove (103). A hydraulic cylinder (116) is further arranged in the bottom groove body. One end of the piston rod of the hydraulic cylinder (116) is connected to the wavy support ring (117). The wavy support ring (117) is in contact with the bottom of the bearing sleeve (105).

7. The multi-stage centrifugation device prepared from the tumor radiotherapy-resistant cell line according to claim 1, characterized in that: The pressing mechanism (5) includes an upper pressing sleeve (501) which is rotatably arranged at the bottom of the upper pressing plate (2). The upper pressing sleeve (501) corresponds to the bearing sleeve (105). The upper pressing plate (2) is rotatably arranged on the mounting frame (3) through a bearing shaft (21).

8. The multi-stage centrifugation device prepared from the tumor radiotherapy-resistant cell line according to claim 7, characterized in that: An upper support sleeve (521) is movably arranged in the upper pressing sleeve (501). An upper pressing plate (503) is further arranged in the upper support sleeve (521). A conical sealing pad (502) is arranged at the bottom of the upper pressing plate (503). Positioning blocks (523) are arranged on both sides of the upper support sleeve (521). One end of each positioning block (523) is slidably arranged in a bearing groove. A second spring (522) is arranged on one side surface of the bearing groove. One end of the second spring (522) is connected to the positioning block (523).

9. The multi-stage centrifugation device prepared from the tumor radiotherapy-resistant cell line according to claim 7, characterized in that: A bottom support (504) is arranged at the inner bottom of the bearing sleeve (105). Limit blocks (513) are arranged on both sides of the bottom support (504). One end of each limit block (513) is slidably arranged in a limit groove (515). The limit grooves (515) are arranged on both sides inside the bearing sleeve (105). A lower spring (514) is further arranged in each limit groove (515). One end of the lower spring (514) is connected to the limit block (513). Grooves (511) are arranged on both sides inside the bearing sleeve (105). A clamping and positioning assembly that moves along with the limit block (513) is arranged in the grooves (511).

10. The multi-stage centrifugation device prepared from the tumor radiotherapy-resistant cell line according to claim 9, characterized in that: The clamping and positioning assembly includes a lower ear seat (512) which is arranged on one side of the limit block (513). A pull rod (506) is rotatably arranged in the lower ear seat (512). One end of the pull rod (506) is rotatably connected to an upper ear seat (516). The upper ear seat (516) is arranged on one side of a clamping arm (509). The clamping arm (509) is rotatably arranged in the groove (511). One end of the clamping arm (509) is connected to a fixed pad (510). A groove (519) is arranged on one side surface of the fixed pad (510). An upper spring (520) is arranged in the groove (519). One end of the upper spring (520) is connected to a positioning pin (518). The positioning pin (518) is movably arranged in the groove (519).

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